@article {pmid31412470,
year = {2019},
author = {Legrand, B and Miras, Y and Beauger, A and Dussauze, M and Latour, D},
title = {Akinetes and ancient DNA reveal toxic cyanobacterial recurrences and their potential for resurrection in a 6700-year-old core from a eutrophic lake.},
journal = {The Science of the total environment},
volume = {687},
number = {},
pages = {1369-1380},
doi = {10.1016/j.scitotenv.2019.07.100},
pmid = {31412470},
issn = {1879-1026},
abstract = {In order to evaluate the recurrence of toxic cyanobacterial blooms and to determine the survival capabilities of the resistance cells through time, a sedimentary core spanning 6700 years was drilled in the eutrophic Lake Aydat. A multiproxy approach (density, magnetic susceptibility, XRF, pollen and non-pollen palynomorph analyses), was used initially to determine the sedimentation model and the land uses around the lake. Comparison with the akinete count revealed that Nostocales cyanobacteria have been present in Lake Aydat over a six thousand year period. This long-term cyanobacterial recurrence also highlights the past presence of both the anaC and mcyB genes, involved in anatoxin-a and microcystin biosynthesis, respectively, throughout the core. The first appearance of cyanobacteria seems to be linked to the natural damming of the river, while the large increase in akinete density around 1800 cal.yr BP can be correlated with the intensification of human activities (woodland clearance, crop planting, grazing, etc.) in the catchment area of the lake, and marks the beginning of a long period of eutrophication. This first investigation into the viability and germination potential of cyanobacteria over thousands of years reveals the ability of intact akinetes to undergo cell divisions even after 1800 years of sedimentation, which is 10 times longer than previously observed. This exceptional cellular resistance, coupled with the long-term eutrophic conditions of this lake, could partly explain the past and current recurrences of cyanobacterial proliferations.},
}

@article {pmid30366251,
year = {2018},
author = {Molinaro, L and Pagani, L},
title = {Human evolutionary history of Eastern Africa.},
journal = {Current opinion in genetics & development},
volume = {53},
number = {},
pages = {134-139},
doi = {10.1016/j.gde.2018.10.002},
pmid = {30366251},
issn = {1879-0380},
mesh = {Africa, Eastern/epidemiology ; Animals ; Anthropology/trends ; *Biological Evolution ; *DNA, Ancient ; Fossils ; Hominidae/*genetics ; Humans ; Paleontology/trends ; },
abstract = {Since the discovery of the first hominin fossils, East Africa has been in the spotlight of palaeo-anthropological investigation for its role as a potential cradle of humanity and as a gateway out of Africa. With the advent of the genomic era an ever increasing amount of information has started to complement this notion, and to place the area within a broader, Pan-African scenario. Here we examine the most recent genetic and fossil results that recapitulate the last hundreds of thousands of years of human evolution in the area, and point to a number of uncharted avenues that may complement the emerging scenario in the coming years.},
}

Africa, Eastern/epidemiology
Animals
Anthropology/trends
*Biological Evolution
*DNA, Ancient
Fossils
Hominidae/*genetics
Humans
Paleontology/trends

@article {pmid30081254,
year = {2018},
author = {Veeramah, KR},
title = {The importance of fine-scale studies for integrating paleogenomics and archaeology.},
journal = {Current opinion in genetics & development},
volume = {53},
number = {},
pages = {83-89},
doi = {10.1016/j.gde.2018.07.007},
pmid = {30081254},
issn = {1879-0380},
mesh = {Archaeology/*trends ; Cultural Evolution ; DNA, Ancient ; Genetics, Population/*trends ; *Genomics ; *Human Migration ; Humans ; Paleontology/trends ; },
abstract = {There has been an undercurrent of intellectual tension between geneticists studying human population history and archaeologists for almost 40 years. The rapid development of paleogenomics, with geneticists working on the very material discovered by archaeologists, appears to have recently heightened this tension. The relationship between these two fields thus far has largely been of a multidisciplinary nature, with archaeologists providing the raw materials for sequencing, as well as a scaffold of hypotheses based on interpretation of archaeological cultures from which the geneticists can ground their inferences from the genomic data. Much of this work has taken place in the context of western Eurasia, which is acting as testing ground for the interaction between the disciplines. Perhaps the major finding has not been any particular historical episode, but rather the apparent pervasiveness of migration events, some apparently of substantial scale, over the past ∼5000 years, challenging the prevailing view of archaeology that largely dismissed migration as a driving force of cultural change in the 1960s. However, while the genetic evidence for `migration' is generally statistically sound, the description of these events as structured behaviours is lacking, which, coupled with often over simplistic archaeological definitions, prevents the use of this information by archaeologists for studying the social processes they are interested in. In order to integrate paleogenomics and archaeology in a truly interdisciplinary manner, it will be necessary to focus less on grand narratives over space and time, and instead integrate genomic data with other form of archaeological information at the level of individual communities to understand the internal social dynamics, which can then be connected amongst communities to model migration at a regional level. A smattering of recent studies have begun to follow this approach, resulting in inferences that are not only helping ask questions that are currently relevant to archaeologists, but also potentially opening up new avenues of research.},
}

Archaeology/*trends
Cultural Evolution
DNA, Ancient
Genetics, Population/*trends
*Genomics
*Human Migration
Humans
Paleontology/trends

@article {pmid31397436,
year = {2019},
author = {Kornienko, IV and Faleeva, TG and Oreshkova, NV and Grigoriev, SE and Grigorieva, LV and Putintseva, YA and Krutovsky, KV},
title = {[Structural and Functional Organization of the Mitochondrial DNA Control Region in the Woolly Mammoth (Mammuthus primigenius)].},
journal = {Molekuliarnaia biologiia},
volume = {53},
number = {4},
pages = {627-637},
doi = {10.1134/S0026898419040062},
pmid = {31397436},
issn = {0026-8984},
abstract = {The woolly mammoth mitochondrial genome (including the Malolyakhovsky mammoth) has been previously sequenced, followed by the annotation of all its genes (MF770243). In this study, based on the Malolyakhovsky mammoth, we describe for the first time the sites of functional significance in the control region of the woolly mammoth mitogenome.},
}

@article {pmid28166504,
year = {2019},
author = {Luhmann, N and Lafond, M and Thevenin, A and Ouangraoua, A and Wittler, R and Chauve, C},
title = {The SCJ Small Parsimony Problem for Weighted Gene Adjacencies.},
journal = {IEEE/ACM transactions on computational biology and bioinformatics},
volume = {16},
number = {4},
pages = {1364-1373},
doi = {10.1109/TCBB.2017.2661761},
pmid = {28166504},
issn = {1557-9964},
abstract = {Reconstructing ancestral gene orders in a given phylogeny is a classical problem in comparative genomics. Most existing methods compare conserved features in extant genomes in the phylogeny to define potential ancestral gene adjacencies, and either try to reconstruct all ancestral genomes under a global evolutionary parsimony criterion, or, focusing on a single ancestral genome, use a scaffolding approach to select a subset of ancestral gene adjacencies, generally aiming at reducing the fragmentation of the reconstructed ancestral genome. In this paper, we describe an exact algorithm for the Small Parsimony Problem that combines both approaches. We consider that gene adjacencies at internal nodes of the species phylogeny are weighted, and we introduce an objective function defined as a convex combination of these weights and the evolutionary cost under the Single-Cut-or-Join (SCJ) model. The weights of ancestral gene adjacencies can, e.g., be obtained through the recent availability of ancient DNA sequencing data, which provide a direct hint at the genome structure of the considered ancestor, or through probabilistic analysis of gene adjacencies evolution. We show the NP-hardness of our problem variant and propose a Fixed-Parameter Tractable algorithm based on the Sankoff-Rousseau dynamic programming algorithm that also allows to sample co-optimal solutions. We apply our approach to mammalian and bacterial data providing different degrees of complexity. We show that including adjacency weights in the objective has a significant impact in reducing the fragmentation of the reconstructed ancestral gene orders. An implementation is available at http://github.com/nluhmann/PhySca.},
}

@article {pmid31389360,
year = {2019},
author = {DAS, R},
title = {OnPeopling of India: Ancient DNA perspectives By K Thangaraj and Niraj Rai.},
journal = {Journal of biosciences},
volume = {44},
number = {3},
pages = {},
pmid = {31389360},
issn = {0973-7138},
}

@article {pmid31389359,
year = {2019},
author = {Thangaraj, K and Rai, N},
title = {Peopling of India: Ancient DNA perspectives.},
journal = {Journal of biosciences},
volume = {44},
number = {3},
pages = {},
pmid = {31389359},
issn = {0973-7138},
abstract = {To reconstruct and explain patterns of genetic diversity of modern humans, understanding their past and present genetic profile is crucial. While genomes of contemporary people can provide information about present day population structure, analysis of ancient genomes may provide unprecedented insights about the past demographic events that have shaped the contemporary gene pool. Population genetics has recently witnessed an explosion in studies on ancient human population histories, primarily from Europe and America. South Asia has no representation in the ancient genomics literature, despite the wealth of archaeological richness in the form of human skeletal remains that exist in collections all over the country. Representing one-fifth of present day humanity calls for understanding the demographic history of south Asia not merely as a prerequisite but as an urgent need to understand its genetic variations on a global scale. Although the overall picture is taking form, new archaeological and genetic information from the region has started to reveal a more complex scenario of ancient human migrations and admixtures than was ever known before. In this article, we discuss a meaningful insight on the current status of ancient DNA (aDNA) research in India. We have also summarized a few but important aDNA studies, which have been successfully carried out in India. Furthermore, we have highlighted the potential opportunity of aDNA research in the Indian subcontinent.},
}

@article {pmid31386837,
year = {2019},
author = {Valente, L and Etienne, RS and Garcia-R, JC},
title = {Deep Macroevolutionary Impact of Humans on New Zealand's Unique Avifauna.},
journal = {Current biology : CB},
volume = {29},
number = {15},
pages = {2563-2569.e4},
doi = {10.1016/j.cub.2019.06.058},
pmid = {31386837},
issn = {1879-0445},
abstract = {Islands are at the frontline of the anthropogenic extinction crisis [1]. A vast number of island birds have gone extinct since human colonization [2], and an important proportion is currently threatened with extinction [3]. While the number of lost or threatened avian species has often been quantified [4], the macroevolutionary consequences of human impact on island biodiversity have rarely been measured [5]. Here, we estimate the amount of evolutionary time that has been lost or is under threat due to anthropogenic activity in a classic example, New Zealand. Half of its bird taxa have gone extinct since humans arrived [6, 7] and many are threatened [8], including lineages forming highly distinct branches in the avian tree of life [9-11]. Using paleontological and ancient DNA information, we compiled a dated phylogenetic dataset for New Zealand's terrestrial avifauna. We extend the method DAISIE developed for island biogeography [12] to allow for the fact that many of New Zealand's birds are evolutionarily isolated and use it to estimate natural rates of speciation, extinction, and colonization. Simulating under a range of human-induced extinction scenarios, we find that it would take approximately 50 million years (Ma) to recover the number of species lost since human colonization of New Zealand and up to 10 Ma to return to today's species numbers if currently threatened species go extinct. This study puts into macroevolutionary perspective the impact of humans in an isolated fauna and reveals how conservation decisions we take today will have repercussions for millions of years.},
}

@article {pmid31381968,
year = {2019},
author = {Oswald, JA and Allen, JM and Witt, KE and Folk, RA and Albury, NA and Steadman, DW and Guralnick, RP},
title = {Ancient DNA from a 2,500-year-old Caribbean fossil places an extinct bird (Caracara creightoni) in a phylogenetic context.},
journal = {Molecular phylogenetics and evolution},
volume = {},
number = {},
pages = {106576},
doi = {10.1016/j.ympev.2019.106576},
pmid = {31381968},
issn = {1095-9513},
abstract = {Since the late Pleistocene humans have caused the extinction of species across our planet. Placing these extinct species in the tree of life with genetic data is essential to understand the ecological and evolutionary implications of these losses. While ancient DNA (aDNA) techniques have advanced rapidly in recent decades, aDNA from tropical species, especially birds, has been historically difficult to obtain, leaving a gap in our understanding of the extinction processes that have influenced current distributions and biodiversity. Here we report the recovery of a nearly complete mitochondrial genome from a 2,500 year old (late Holocene) bone of an extinct species of bird, Caracara creightoni, recovered from the anoxic saltwater environment of a blue hole in the Bahamas. Our results suggest that this extinct species is sister (1.6% sequence divergence) to a clade containing the extant C. cheriway and C. plancus. Caracara creightoni shared a common ancestor with these extant species during the Pleistocene (1.2-0.4 MYA) and presumably survived on Cuba when the Bahamas was mostly underwater during Quaternary interglacial intervals (periods of high sea levels). Tropical blue holes have been collecting animals for thousands of years and will continue to improve our understanding of faunal extinctions and distributions. In particular, new aDNA techniques combined with radiocarbon dating from Holocene Bahamian fossils will allow us to place other extinct (species-level loss) and extirpated (population-level loss) vertebrate taxa in improved phylogenetic, evolutionary, biogeographic, and temporal contexts.},
}

@article {pmid31361744,
year = {2019},
author = {Smith, O and Dunshea, G and Sinding, MS and Fedorov, S and Germonpre, M and Bocherens, H and Gilbert, MTP},
title = {Ancient RNA from Late Pleistocene permafrost and historical canids shows tissue-specific transcriptome survival.},
journal = {PLoS biology},
volume = {17},
number = {7},
pages = {e3000166},
doi = {10.1371/journal.pbio.3000166},
pmid = {31361744},
issn = {1545-7885},
abstract = {While sequencing ancient DNA (aDNA) from archaeological material is now commonplace, very few attempts to sequence ancient transcriptomes have been made, even from typically stable deposition environments such as permafrost. This is presumably due to assumptions that RNA completely degrades relatively quickly, particularly when dealing with autolytic, nuclease-rich mammalian tissues. However, given the recent successes in sequencing ancient RNA (aRNA) from various sources including plants and animals, we suspect that these assumptions may be incorrect or exaggerated. To challenge the underlying dogma, we generated shotgun RNA data from sources that might normally be dismissed for such study. Here, we present aRNA data generated from two historical wolf skins, and permafrost-preserved liver tissue of a 14,300-year-old Pleistocene canid. Not only is the latter the oldest RNA ever to be sequenced, but it also shows evidence of biologically relevant tissue specificity and close similarity to equivalent data derived from modern-day control tissue. Other hallmarks of RNA sequencing (RNA-seq) data such as exon-exon junction presence and high endogenous ribosomal RNA (rRNA) content confirms our data's authenticity. By performing independent technical library replicates using two high-throughput sequencing platforms, we show not only that aRNA can survive for extended periods in mammalian tissues but also that it has potential for tissue identification. aRNA also has possible further potential, such as identifying in vivo genome activity and adaptation, when sequenced using this technology.},
}

@article {pmid31353181,
year = {2019},
author = {Ning, C and Wang, CC and Gao, S and Yang, Y and Zhang, X and Wu, X and Zhang, F and Nie, Z and Tang, Y and Robbeets, M and Ma, J and Krause, J and Cui, Y},
title = {Ancient Genomes Reveal Yamnaya-Related Ancestry and a Potential Source of Indo-European Speakers in Iron Age Tianshan.},
journal = {Current biology : CB},
volume = {},
number = {},
pages = {},
doi = {10.1016/j.cub.2019.06.044},
pmid = {31353181},
issn = {1879-0445},
abstract = {Recent studies of early Bronze Age human genomes revealed a massive population expansion by individuals-related to the Yamnaya culture, from the Pontic Caspian steppe into Western and Eastern Eurasia, likely accompanied by the spread of Indo-European languages [1-5]. The south eastern extent of this migration is currently not known. Modern-day human populations from the Xinjiang region in northwestern China show a complex population history, with genetic links to both Eastern and Western Eurasia [6-10]. However, due to the lack of ancient genomic data, it remains unclear which source populations contributed to the Xinjiang population and what was the timing and the number of admixture events. Here, we report the first genome-wide data of 10 ancient individuals from northeastern Xinjiang. They are dated to around 2,200 years ago and were found at the Iron Age Shirenzigou site. We find them to be already genetically admixed between Eastern and Western Eurasians. We also find that the majority of the East Eurasian ancestry in the Shirenzigou individuals is-related to northeastern Asian populations, while the West Eurasian ancestry is best presented by ∼20% to 80% Yamnaya-like ancestry. Our data thus suggest a Western Eurasian steppe origin for at least part of the ancient Xinjiang population. Our findings furthermore support a Yamnaya-related origin for the now extinct Tocharian languages in the Tarim Basin, in southern Xinjiang.},
}

@article {pmid31351222,
year = {2019},
author = {Molto, JE and Kirkpatrick, CL and Keron, J},
title = {The paleoepidemiology of Sacral Spina Bifida Occulta in population samples from the Dakhleh Oasis, Egypt.},
journal = {International journal of paleopathology},
volume = {26},
number = {},
pages = {93-103},
doi = {10.1016/j.ijpp.2019.06.006},
pmid = {31351222},
issn = {1879-9825},
abstract = {OBJECTIVE: To document sacral spina bifida occulta (SSB0) prevalence in a population sample from the Dakhleh Oasis, Egypt, and address methodological issues in recording and quantifying SSBO variations.

MATERIALS: 442 adult sacra from two temporally disjunct samples from the same deme traversing the 3rd intermediate (TIP) and the Roman Periods.

METHODS: Sacra were scored for SSBO, excluding the sacral hiatus. Risk of SSBO was calculated with the common odds ratio and statistical significance by X2. Data were compared to other archaeological SSBO data.

RESULTS: SSBO was present in 15.6% of the sample with a slight, but not significant, temporal increase (TIP to Roman Period) in males, and a significant age-correlated increase in both sexes. Most open sacra occurred in young adults.

CONCLUSIONS: Data support that SSBO can be considered as a morphogenetic variant. Dakhleh data fall within the prevalence range for most populations, however inter-population comparisons are complicated by methodological inconsistencies.

SIGNIFICANCE: SSBO can be used in paleogenetic research.

LIMITATIONS: Methodological differences in scoring SSBO prevent effective comparative study.

SUGGESTED FUTURE RESEARCH: Future studies require more rigorous and standardized scoring methods. aDNA may be used to corroborate the morphogenetic value of SSBO and determine its clinical significance.},
}

@article {pmid31348818,
year = {2019},
author = {Günther, T and Nettelblad, C},
title = {The presence and impact of reference bias on population genomic studies of prehistoric human populations.},
journal = {PLoS genetics},
volume = {15},
number = {7},
pages = {e1008302},
doi = {10.1371/journal.pgen.1008302},
pmid = {31348818},
issn = {1553-7404},
abstract = {High quality reference genomes are an important resource in genomic research projects. A consequence is that DNA fragments carrying the reference allele will be more likely to map successfully, or receive higher quality scores. This reference bias can have effects on downstream population genomic analysis when heterozygous sites are falsely considered homozygous for the reference allele. In palaeogenomic studies of human populations, mapping against the human reference genome is used to identify endogenous human sequences. Ancient DNA studies usually operate with low sequencing coverages and fragmentation of DNA molecules causes a large proportion of the sequenced fragments to be shorter than 50 bp-reducing the amount of accepted mismatches, and increasing the probability of multiple matching sites in the genome. These ancient DNA specific properties are potentially exacerbating the impact of reference bias on downstream analyses, especially since most studies of ancient human populations use pseudo-haploid data, i.e. they randomly sample only one sequencing read per site. We show that reference bias is pervasive in published ancient DNA sequence data of prehistoric humans with some differences between individual genomic regions. We illustrate that the strength of reference bias is negatively correlated with fragment length. Most genomic regions we investigated show little to no mapping bias but even a small proportion of sites with bias can impact analyses of those particular loci or slightly skew genome-wide estimates. Therefore, reference bias has the potential to cause minor but significant differences in the results of downstream analyses such as population allele sharing, heterozygosity estimates and estimates of archaic ancestry. These spurious results highlight how important it is to be aware of these technical artifacts and that we need strategies to mitigate the effect. Therefore, we suggest some post-mapping filtering strategies to resolve reference bias which help to reduce its impact substantially.},
}

@article {pmid31328768,
year = {2019},
author = {Mas-Sandoval, A and Arauna, LR and Gouveia, MH and Barreto, ML and Horta, BL and Lima-Costa, MF and Pereira, AC and Salzano, FM and Hünemeier, T and Tarazona-Santos, E and Bortolini, MC and Comas, D},
title = {Reconstructed lost Native American populations from Eastern Brazil are shaped by differential Jê/Tupi ancestry.},
journal = {Genome biology and evolution},
volume = {},
number = {},
pages = {},
doi = {10.1093/gbe/evz161},
pmid = {31328768},
issn = {1759-6653},
abstract = {After the colonization of the Americas by Europeans and the consequent Trans-Atlantic Slave Trade, most Native American populations in eastern Brazil disappeared or went through an admixture process that configured a population composed of three main genetic components: the European, the sub-Saharan African and the Native American. The study of the Native American genetic history is challenged by the availability of genome-wide samples from Native American populations, the technical difficulties to develop ancient DNA studies and the low proportions of the Native American component in the admixed Brazilian populations (in average 7%). We analysed genome-wide data of 5,825 individuals from three locations of eastern Brazil: Salvador (North East), Bambui (South East), and Pelotas (South) and we reconstructed populations that emulate the Native American groups that were living in the 16th century around the sampling locations. This genetic reconstruction was performed after local ancestry analysis of the admixed Brazilian populations, through the rearrangement of the Native American haplotypes into reconstructed individuals with full Native American ancestry (51 reconstructed individuals in Salvador, 45 in Bambui and 197 in Pelotas). We compared the reconstructed populations with non-admixed Native American populations from other regions of Brazil through haplotype-based methods. Our results reveal a population structure shaped by the dichotomy of Tupi-/Jê- speaking ancestry related groups. We also show evidence of a decrease of the diversity of non-admixed Native American groups after the European contact, in contrast with the reconstructed populations, suggesting a reservoir of the Native American genetic diversity within the admixed Brazilian population.},
}

@article {pmid31318917,
year = {2019},
author = {Prufer, KM and Alsgaard, AV and Robinson, M and Meredith, CR and Culleton, BJ and Dennehy, T and Magee, S and Huckell, BB and Stemp, WJ and Awe, JJ and Capriles, JM and Kennett, DJ},
title = {Linking late Paleoindian stone tool technologies and populations in North, Central and South America.},
journal = {PloS one},
volume = {14},
number = {7},
pages = {e0219812},
doi = {10.1371/journal.pone.0219812},
pmid = {31318917},
issn = {1932-6203},
abstract = {From the perspective of Central and South America, the peopling of the New World was a complex process lasting thousands of years and involving multiple waves of Pleistocene and early Holocene period immigrants entering into the neotropics. These Paleoindian colonists initially brought with them technologies developed for adaptation to environments and resources found in North America. As the ice age ended across the New World people adapted more generalized stone tools to exploit changing environments and resources. In the neotropics these changes would have been pronounced as patchy forests and grasslands gave way to broadleaf tropical forests. We document a late Pleistocene/early Holocene stone tool tradition from Belize, located in southern Mesoamerica. This represents the first endogenous Paleoindian stone tool technocomplex recovered from well dated stratigraphic contexts for Mesoamerica. Previously designated Lowe, these artifacts share multiple features with contemporary North and South American Paleoindian tool types. Once hafted, these bifaces appear to have served multiple functions for cutting, hooking, thrusting, or throwing. The tools were developed at a time of technological regionalization reflecting the diverse demands of a period of pronounced environmental change and population movement. Combined stratigraphic, technological, and population paleogenetic data suggests that there were strong ties between lowland neotropic regions at the onset of the Holocene.},
}

@article {pmid31310584,
year = {2019},
author = {Hong, JH and Seo, M and Oh, CS and Shin, DH},
title = {Genetic Analysis of Small-Subunit Ribosomal RNA, Internal Transcribed Spacer 2, and ATP Synthase Subunit 8 of Trichuris trichiura Ancient DNA Retrieved from the 15th to 18th Century Joseon Dynasty Mummies' Coprolites from Korea.},
journal = {The Journal of parasitology},
volume = {105},
number = {4},
pages = {539-545},
pmid = {31310584},
issn = {1937-2345},
abstract = {Although parasitic infection by Trichuris trichiura is a very common intestinal helminthic disease worldwide, there is still insufficient information on the genetic characteristics of ancient T. trichiura in different spatiotemporal perspectives. Utilizing coprolite specimens obtained from 15th-18th century mummies dating to the Joseon Dynasty, we analyzed small-subunit ribosomal RNA, internal transcribed spacer 2, and ATP synthase subunit 8 of T. trichiura ancient DNA (aDNA). In BLAST and phylogenetic analyses, the T. trichiura aDNA sequences of this study belong to a separate cluster that is evidently distinct from the other genus Trichuris spp. reported in GenBank. This report characterizes T. trichiura aDNA of pre-20th century East Asia, and in so doing, it also proves the potential of aDNA analysis for differential diagnosis of T. trichiura in cases where ancient parasite eggs are morphologically indeterminate for species identification.},
}

@article {pmid31308224,
year = {2019},
author = {Bokelmann, L and Hajdinjak, M and Peyrégne, S and Brace, S and Essel, E and de Filippo, C and Glocke, I and Grote, S and Mafessoni, F and Nagel, S and Kelso, J and Prüfer, K and Vernot, B and Barnes, I and Pääbo, S and Meyer, M and Stringer, C},
title = {A genetic analysis of the Gibraltar Neanderthals.},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {116},
number = {31},
pages = {15610-15615},
doi = {10.1073/pnas.1903984116},
pmid = {31308224},
issn = {1091-6490},
abstract = {The Forbes' Quarry and Devil's Tower partial crania from Gibraltar are among the first Neanderthal remains ever found. Here, we show that small amounts of ancient DNA are preserved in the petrous bones of the 2 individuals despite unfavorable climatic conditions. However, the endogenous Neanderthal DNA is present among an overwhelming excess of recent human DNA. Using improved DNA library construction methods that enrich for DNA fragments carrying deaminated cytosine residues, we were able to sequence 70 and 0.4 megabase pairs (Mbp) nuclear DNA of the Forbes' Quarry and Devil's Tower specimens, respectively, as well as large parts of the mitochondrial genome of the Forbes' Quarry individual. We confirm that the Forbes' Quarry individual was a female and the Devil's Tower individual a male. We also show that the Forbes' Quarry individual is genetically more similar to the ∼120,000-y-old Neanderthals from Scladina Cave in Belgium (Scladina I-4A) and Hohlenstein-Stadel Cave in Germany, as well as to a ∼60,000- to 70,000-y-old Neanderthal from Russia (Mezmaiskaya 1), than to a ∼49,000-y-old Neanderthal from El Sidrón (El Sidrón 1253) in northern Spain and other younger Neanderthals from Europe and western Asia. This suggests that the Forbes' Quarry fossil predates the latter Neanderthals. The preservation of archaic human DNA in the warm coastal climate of Gibraltar, close to the shores of Africa, raises hopes for the future recovery of archaic human DNA from regions in which climatic conditions are less than optimal for DNA preservation.},
}

@article {pmid31303491,
year = {2019},
author = {Järve, M and Saag, L and Scheib, CL and Pathak, AK and Montinaro, F and Pagani, L and Flores, R and Guellil, M and Saag, L and Tambets, K and Kushniarevich, A and Solnik, A and Varul, L and Zadnikov, S and Petrauskas, O and Avramenko, M and Magomedov, B and Didenko, S and Toshev, G and Bruyako, I and Grechko, D and Okatenko, V and Gorbenko, K and Smyrnov, O and Heiko, A and Reida, R and Sapiehin, S and Sirotin, S and Tairov, A and Beisenov, A and Starodubtsev, M and Vasilev, V and Nechvaloda, A and Atabiev, B and Litvinov, S and Ekomasova, N and Dzhaubermezov, M and Voroniatov, S and Utevska, O and Shramko, I and Khusnutdinova, E and Metspalu, M and Savelev, N and Kriiska, A and Kivisild, T and Villems, R},
title = {Shifts in the Genetic Landscape of the Western Eurasian Steppe Associated with the Beginning and End of the Scythian Dominance.},
journal = {Current biology : CB},
volume = {29},
number = {14},
pages = {2430-2441.e10},
doi = {10.1016/j.cub.2019.06.019},
pmid = {31303491},
issn = {1879-0445},
abstract = {The Early Iron Age nomadic Scythians have been described as a confederation of tribes of different origins, based on ancient DNA evidence [1-3]. It is still unclear how much of the Scythian dominance in the Eurasian Steppe was due to movements of people and how much reflected cultural diffusion and elite dominance. We present new whole-genome sequences of 31 ancient Western and Eastern Steppe individuals, including Scythians as well as samples pre- and postdating them, allowing us to set the Scythians in a temporal context (in the Western, i.e., Ponto-Caspian Steppe). We detect an increase of eastern (Altaian) affinity along with a decrease in eastern hunter-gatherer (EHG) ancestry in the Early Iron Age Ponto-Caspian gene pool at the start of the Scythian dominance. On the other hand, samples of the Chernyakhiv culture postdating the Scythians in Ukraine have a significantly higher proportion of Near Eastern ancestry than other samples of this study. Our results agree with the Gothic source of the Chernyakhiv culture and support the hypothesis that the Scythian dominance did involve a demic component.},
}

@article {pmid31303107,
year = {2019},
author = {Houldcroft, CJ and Rifkin, RF and Underdown, SJ},
title = {Human biology and ancient DNA: exploring disease, domestication and movement.},
journal = {Annals of human biology},
volume = {46},
number = {2},
pages = {95-98},
doi = {10.1080/03014460.2019.1629536},
pmid = {31303107},
issn = {1464-5033},
}

@article {pmid31300536,
year = {2019},
author = {Teixeira, JC and Cooper, A},
title = {Using hominin introgression to trace modern human dispersals.},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {116},
number = {31},
pages = {15327-15332},
doi = {10.1073/pnas.1904824116},
pmid = {31300536},
issn = {1091-6490},
abstract = {The dispersal of anatomically modern human populations out of Africa and across much of the rest of the world around 55 to 50 thousand years before present (ka) is recorded genetically by the multiple hominin groups they met and interbred with along the way, including the Neandertals and Denisovans. The signatures of these introgression events remain preserved in the genomes of modern-day populations, and provide a powerful record of the sequence and timing of these early migrations, with Asia proving a particularly complex area. At least 3 different hominin groups appear to have been involved in Asia, of which only the Denisovans are currently known. Several interbreeding events are inferred to have taken place east of Wallace's Line, consistent with archaeological evidence of widespread and early hominin presence in the area. However, archaeological and fossil evidence indicates archaic hominins had not spread as far as the Sahul continent (New Guinea, Australia, and Tasmania), where recent genetic evidence remains enigmatic.},
}

@article {pmid31297530,
year = {2019},
author = {Koch, E and Schweizer, RM and Schweizer, TM and Stahler, DR and Smith, DW and Wayne, RK and Novembre, J},
title = {De novo mutation rate estimation in wolves of known pedigree.},
journal = {Molecular biology and evolution},
volume = {},
number = {},
pages = {},
doi = {10.1093/molbev/msz159},
pmid = {31297530},
issn = {1537-1719},
abstract = {Knowledge of mutation rates is crucial for calibrating population genetics models of demographic history in units of years. However, mutation rates remain challenging to estimate because of the need to identify extremely rare events. We estimated the nuclear mutation rate in wolves by identifying de novo mutations in a pedigree of seven wolves. Putative de novo mutations were discovered by whole-genome sequencing and were verified by Sanger sequencing of parents and offspring. Using stringent filters and an estimate of the false negative rate in the remaining observable genome, we obtain an estimate of ∼4.5 x 10-9 per base pair per generation and provide conservative bounds from 2.6 x 10-9 and 7.1 x 10-9. Although our estimate is consistent with recent mutation rate estimates from ancient DNA (4.0 x 10-9 and 3.0-4.5 x 10-9), it implies a wider possible range. We also examined the consequences of our rate and the accompanying interval for dating several critical events in canid demographic history. For example, applying our full range of rates to coalescent models of dog and wolf demographic history implies a wide set of possible divergence times between the ancestral populations of dogs and extant Eurasian wolves (16,000 - 64,000 years ago) although our point estimate indicates a date between 25,000 and 33,000 years ago. Aside from one study in mice, ours provides the only direct mammalian mutation rate outside of primates, and is likely to be vital to future investigations of mutation rate evolution.},
}

@article {pmid31296442,
year = {2019},
author = {Cornille, A and Antolín, F and Garcia, E and Vernesi, C and Fietta, A and Brinkkemper, O and Kirleis, W and Schlumbaum, A and Roldán-Ruiz, I},
title = {A Multifaceted Overview of Apple Tree Domestication.},
journal = {Trends in plant science},
volume = {24},
number = {8},
pages = {770-782},
doi = {10.1016/j.tplants.2019.05.007},
pmid = {31296442},
issn = {1878-4372},
abstract = {The apple is an iconic tree and a major fruit crop worldwide. It is also a model species for the study of the evolutionary processes and genomic basis underlying the domestication of clonally propagated perennial crops. Multidisciplinary approaches from across Eurasia have documented the pace and process of cultivation of this remarkable crop. While population genetics and genomics have revealed the overall domestication history of apple across Eurasia, untangling the evolutionary processes involved, archeobotany has helped to document the transition from gathering and using apples to the practice of cultivation. Further studies integrating archeogenetic and archeogenomic approaches will bring new insights about key traits involved in apple domestication. Such knowledge has potential to boost innovation in present-day apple breeding.},
}

@article {pmid31291259,
year = {2019},
author = {Zimmermann, HH and Harms, L and Epp, LS and Mewes, N and Bernhardt, N and Kruse, S and Stoof-Leichsenring, KR and Pestryakova, LA and Wieczorek, M and Trense, D and Herzschuh, U},
title = {Chloroplast and mitochondrial genetic variation of larches at the Siberian tundra-taiga ecotone revealed by de novo assembly.},
journal = {PloS one},
volume = {14},
number = {7},
pages = {e0216966},
doi = {10.1371/journal.pone.0216966},
pmid = {31291259},
issn = {1932-6203},
abstract = {Larix populations at the tundra-taiga ecotone in northern Siberia are highly under-represented in population genetic studies, possibly due to the remoteness of these regions that can only be accessed at extraordinary expense. The genetic signatures of populations in these boundary regions are therefore largely unknown. We aim to generate organelle reference genomes for the detection of single nucleotide polymorphisms (SNPs) that can be used for paleogenetic studies. We present 19 complete chloroplast genomes and mitochondrial genomic sequences of larches from the southern lowlands of the Taymyr Peninsula (northernmost range of Larix gmelinii (Rupr.) Kuzen.), the lower Omoloy River, and the lower Kolyma River (both in the range of Larix cajanderi Mayr). The genomic data reveal 84 chloroplast SNPs and 213 putatively mitochondrial SNPs. Parsimony-based chloroplast haplotype networks show no spatial structure of individuals from different geographic origins, while the mitochondrial haplotype network shows at least a slight spatial structure with haplotypes from the Omoloy and Kolyma populations being more closely related to each other than to most of the haplotypes from the Taymyr populations. Whole genome alignments with publicly available complete chloroplast genomes of different Larix species show that among official plant barcodes only the rcbL gene contains sufficient polymorphisms, but has to be sequenced completely to distinguish the different provenances. We provide 8 novel mitochondrial SNPs that are putatively diagnostic for the separation of L. gmelinii and L. cajanderi, while 4 chloroplast SNPs have the potential to distinguish the L. gmelinii/L. cajanderi group from other Larix species. Our organelle references can be used for a targeted primer and probe design allowing the generation of short amplicons. This is particularly important with regard to future investigations of, for example, the biogeographic history of Larix by screening ancient sedimentary DNA of Larix.},
}

@article {pmid31284503,
year = {2019},
author = {Lan, TM and Lin, Y and Njaramba-Ngatia, J and Guo, XS and Li, RG and Li, HM and Kumar-Sahu, S and Wang, X and Yang, XJ and Guo, HB and Xu, WH and Kristiansen, K and Liu, H and Xu, YC},
title = {Improving Species Identification of Ancient Mammals Based on Next-Generation Sequencing Data.},
journal = {Genes},
volume = {10},
number = {7},
pages = {},
doi = {10.3390/genes10070509},
pmid = {31284503},
issn = {2073-4425},
abstract = {The taxonomical identification merely based on morphology is often difficult for ancient remains. Therefore, universal or specific PCR amplification followed by sequencing and BLAST (basic local alignment search tool) search has become the most frequently used genetic-based method for the species identification of biological samples, including ancient remains. However, it is challenging for these methods to process extremely ancient samples with severe DNA fragmentation and contamination. Here, we applied whole-genome sequencing data from 12 ancient samples with ages ranging from 2.7 to 700 kya to compare different mapping algorithms, and tested different reference databases, mapping similarities and query coverage to explore the best method and mapping parameters that can improve the accuracy of ancient mammal species identification. The selected method and parameters were tested using 152 ancient samples, and 150 of the samples were successfully identified. We further screened the BLAST-based mapping results according to the deamination characteristics of ancient DNA to improve the ability of ancient species identification. Our findings demonstrate a marked improvement to the normal procedures used for ancient species identification, which was achieved through defining the mapping and filtering guidelines to identify true ancient DNA sequences. The guidelines summarized in this study could be valuable in archaeology, paleontology, evolution, and forensic science. For the convenience of the scientific community, we wrote a software script with Perl, called AncSid, which is made available on GitHub.},
}

@article {pmid31281897,
year = {2019},
author = {Feldman, M and Master, DM and Bianco, RA and Burri, M and Stockhammer, PW and Mittnik, A and Aja, AJ and Jeong, C and Krause, J},
title = {Ancient DNA sheds light on the genetic origins of early Iron Age Philistines.},
journal = {Science advances},
volume = {5},
number = {7},
pages = {eaax0061},
doi = {10.1126/sciadv.aax0061},
pmid = {31281897},
issn = {2375-2548},
abstract = {The ancient Mediterranean port city of Ashkelon, identified as "Philistine" during the Iron Age, underwent a marked cultural change between the Late Bronze and the early Iron Age. It has been long debated whether this change was driven by a substantial movement of people, possibly linked to a larger migration of the so-called "Sea Peoples." Here, we report genome-wide data of 10 Bronze and Iron Age individuals from Ashkelon. We find that the early Iron Age population was genetically distinct due to a European-related admixture. This genetic signal is no longer detectible in the later Iron Age population. Our results support that a migration event occurred during the Bronze to Iron Age transition in Ashkelon but did not leave a long-lasting genetic signature.},
}

@article {pmid31270812,
year = {2019},
author = {Fleskes, RE and Bruwelheide, KS and West, FL and Owsley, DW and Griffith, DR and Barca, KG and Cabana, GS and Schurr, TG},
title = {Ancient DNA and bioarchaeological perspectives on European and African diversity and relationships on the colonial Delaware frontier.},
journal = {American journal of physical anthropology},
volume = {},
number = {},
pages = {},
doi = {10.1002/ajpa.23887},
pmid = {31270812},
issn = {1096-8644},
support = {//The University of Pennsylvania/ ; },
abstract = {OBJECTIVES: Ancient DNA (aDNA) and standard osteological analyses applied to 11 skeletons at a late 17th to early 18th century farmstead site in Delaware to investigate the biological and social factors of settlement and slavery in colonial America.

MATERIALS AND METHODS: Osteological analysis and mitochondrial DNA (mtDNA) sequencing were conducted for all individuals and the resulting data contextualized with archaeological and documentary evidence.

RESULTS: Individuals of European and African descent were spatially separated in this colonial cemetery. The skeletal remains exhibited differences in osteological features and maternal genetic ancestry. A specific mtDNA haplotype appeared in a subset of the European-descended individuals suggesting they were maternally related. Individuals of African descent were not maternally related, and instead showed a diversity of haplotypes affiliated with present-day Western, Central, and Eastern regions of Africa.

DISCUSSION: Along with the bioarchaeological and documentary evidence, the aDNA findings contribute to our understanding of life on the colonial Delaware frontier. Evidence of maternal relatedness among European-descended individuals at the site demonstrates kin-based settlements in 17th century Delaware and provides preliminary identifications of individuals. The maternal genetic diversity of the individuals with African descent aligns with the routes of the trans-Atlantic slave trade but broadens our understanding of the ancestries of persons involved in it. Burial positioning, osteological pathology, and lack of maternal kinship among individuals of African descent provide tangible evidence for the emergence of racialized labor and society in Delaware during the late 17th century.},
}

@article {pmid31256878,
year = {2019},
author = {Joseph, TA and Pe'er, I},
title = {Inference of Population Structure from Time-Series Genotype Data.},
journal = {American journal of human genetics},
volume = {},
number = {},
pages = {},
doi = {10.1016/j.ajhg.2019.06.002},
pmid = {31256878},
issn = {1537-6605},
abstract = {Sequencing ancient DNA can offer direct probing of population history. Yet, such data are commonly analyzed with standard tools that assume DNA samples are all contemporary. We present DyStruct, a model and inference algorithm for inferring shared ancestry from temporally sampled genotype data. DyStruct explicitly incorporates temporal dynamics by modeling individuals as mixtures of unobserved populations whose allele frequencies drift over time. We develop an efficient inference algorithm for our model using stochastic variational inference. On simulated data, we show that DyStruct outperforms the current state of the art when individuals are sampled over time. Using a dataset of 296 modern and 80 ancient samples, we demonstrate DyStruct is able to capture a well-supported admixture event of steppe ancestry into modern Europe. We further apply DyStruct to a genome-wide dataset of 2,067 modern and 262 ancient samples used to study the origin of farming in the Near East. We show that DyStruct provides new insight into population history when compared with alternate approaches, within feasible run time.},
}

@article {pmid31251487,
year = {2019},
author = {Angelici, FM and Ciucani, MM and Angelini, S and Annesi, F and Caniglia, R and Castiglia, R and Fabbri, E and Galaverni, M and Palumbo, D and Ravegnini, G and Rossi, L and Siracusa, AM and Cilli, E},
title = {The Sicilian Wolf: Genetic Identity of a Recently Extinct Insular Population.},
journal = {Zoological science},
volume = {36},
number = {3},
pages = {189-197},
doi = {10.2108/zs180180},
pmid = {31251487},
issn = {0289-0003},
abstract = {Historically, many local grey wolf (Canis lupus) populations have undergone substantial reductions in size or become extinct. Among these, the wolf population once living in Sicily, the largest island in the Mediterranean Sea, was completely eradicated by human activity in the early decades of the 20th century. To gain a better understanding of the genetic identity of the Sicilian wolf, we used techniques for the study of ancient DNA to analyze the mitochondrial (mt) variability of six specimens stored in Italian museums. We were able to amplify a diagnostic mtDNA fragment of the control region (CR) in four of the samples. Two of the samples shared the same haplotype, differing by two substitutions from the currently most diffused Italian wolf haplotype (W14) and one substitution from the only other Italian haplotype (W16). The third sample showed a previously unreported wolf-like haplotype, and the fourth a haplotype commonly found in dogs. All of the wolf haplotypes analyzed in this study belonged to the mitochondrial haplogroup that includes haplotypes detected in all the known European Pleistocene wolves and in several modern southern European populations. Unfortunately, this endemic island population, which exhibited unique mtDNA variability, was definitively lost before it was possible to understand its taxonomic uniqueness and conservational value.},
}

@article {pmid31249793,
year = {2019},
author = {Silva, PC and Malabarba, MC and Vari In Memoriam, R and Malabarba, LR},
title = {Comparison and optimization for DNA extraction of archived fish specimens.},
journal = {MethodsX},
volume = {6},
number = {},
pages = {1433-1442},
doi = {10.1016/j.mex.2019.06.001},
pmid = {31249793},
issn = {2215-0161},
abstract = {The DNA extracted from museum alcohol-fixed specimens can be a valuable source of information for solving taxonomic, phylogenetic, ecological and conservational questions. However, this type of DNA, also called ancient DNA, is routinely obtained in small portions and highly fragmented. We have tested two different extraction kits in museum type-specimens of the fish family Characidae. Aiming to increase the DNA yield, we made modifications on a Qiagen manufacturer protocol, in the elution step. Also, to overcome the issue of DNA fragmentation, we applied our efforts in Sanger sequencing, to find a highly variable and, in result, informative COI fragment. Based on our results, there is no correlation between amount of the DNA extracted and the age of the sample. The Sanger sequencing generated sequences which are useful in solving taxonomic puzzles. Here are presented the customization and guidelines that allowed us to recover DNA from the archived fish specimens. •DNA extraction from archived fish specimens is more effective when using silica columns.•Change of the elution times from minutes in room temperature to 24 h in freezer greatly improved the DNA yielded.•Short but highly variable sequences replace the need to sequence the entire gene to identify a species.},
}

@article {pmid31246567,
year = {2019},
author = {Botbayev, D and Ravegnini, G and Sammarini, G and Kazymbet, P and Cilli, E and Serventi, P and Khanseitova, A and Alzhanuly, B and Belkozhaev, A and Aitkhozhina, N and Bakhtin, M and Lodi, V and Hrelia, P and Angelini, S},
title = {Absence of mutations in the human interferon alpha-2b gene in workers chronically exposed to ionising radiation.},
journal = {Arhiv za higijenu rada i toksikologiju},
volume = {70},
number = {2},
pages = {104-108},
doi = {10.2478/aiht-2019-70-3202},
pmid = {31246567},
issn = {1848-6312},
abstract = {Individuals chronically exposed to low-level ionising radiation (IR) run the risk of harmful and long-term adverse health effects, including gene mutations and cancer development. The search for reliable biomarkers of IR exposure in human population is still of great interest, as they may have a great implementation potential for the surveillance of occupationally exposed individuals. In this context, and considering previous literature, this study aimed to identify mutations in the human interferon alpha-2b (hIFNα-2b) as a potential biomarker of occupational chronic low-dose IR exposure linking low-IR exposure to the effects on haematopoiesis and reduced immunity. The analysis was performed in the genomic DNA of 51 uranium miners and 38 controls from Kazakhstan, and in 21 medical radiology workers and 21 controls from Italy. hIFNα-2b gene mutations were analysed with the real-time polymerase chain reaction (PCR) or Sanger sequencing. However, none of the investigated workers had the hIFNα-2b mutation. This finding highlights the need for further research to identify biomarkers for early detection of health effects associated with chronic low-dose IR exposure.},
}

@article {pmid31235841,
year = {2019},
author = {De Schepper, S and Ray, JL and Skaar, KS and Sadatzki, H and Ijaz, UZ and Stein, R and Larsen, A},
title = {The potential of sedimentary ancient DNA for reconstructing past sea ice evolution.},
journal = {The ISME journal},
volume = {},
number = {},
pages = {},
doi = {10.1038/s41396-019-0457-1},
pmid = {31235841},
issn = {1751-7370},
support = {268062//Norges Forskningsråd (Research Council of Norway)/ ; 273455/E10//Norges Forskningsråd (Research Council of Norway)/ ; 610055//EC | EC Seventh Framework Programm | FP7 Ideas: European Research Council (FP7-IDEAS-ERC - Specific Programme: "Ideas" Implementing the Seventh Framework Programme of the European Community for Research, Technological Development and Demonstration Activities (2007 to 2013))/ ; 610055//EC | EC Seventh Framework Programm | FP7 Ideas: European Research Council (FP7-IDEAS-ERC - Specific Programme: "Ideas" Implementing the Seventh Framework Programme of the European Community for Research, Technological Development and Demonstration Activities (2007 to 2013))/ ; NE/L011958/1//RCUK | Natural Environment Research Council (NERC)/ ; Lord Kelvin Adam Smith Leadership Fellowship//University of Glasgow/ ; },
abstract = {Sea ice is a crucial component of the Arctic climate system, yet the tools to document the evolution of sea ice conditions on historical and geological time scales are few and have limitations. Such records are essential for documenting and understanding the natural variations in Arctic sea ice extent. Here we explore sedimentary ancient DNA (aDNA), as a novel tool that unlocks and exploits the genetic (eukaryote) biodiversity preserved in marine sediments specifically for past sea ice reconstructions. Although use of sedimentary aDNA in paleoceanographic and paleoclimatic studies is still in its infancy, we use here metabarcoding and single-species quantitative DNA detection methods to document the sea ice conditions in a Greenland Sea marine sediment core. Metabarcoding has allowed identifying biodiversity changes in the geological record back to almost ~100,000 years ago that were related to changing sea ice conditions. Detailed bioinformatic analyses on the metabarcoding data revealed several sea-ice-associated taxa, most of which previously unknown from the fossil record. Finally, we quantitatively traced one known sea ice dinoflagellate in the sediment core. We show that aDNA can be recovered from deep-ocean sediments with generally oxic bottom waters and that past sea ice conditions can be documented beyond instrumental time scales. Our results corroborate sea ice reconstructions made by traditional tools, and thus demonstrate the potential of sedimentary aDNA, focusing primarily on microbial eukaryotes, as a new tool to better understand sea ice evolution in the climate system.},
}

@article {pmid31223116,
year = {2019},
author = {Eisenhofer, R and Cooper, A},
title = {A new home for microbes.},
journal = {eLife},
volume = {8},
number = {},
pages = {},
doi = {10.7554/eLife.48493},
pmid = {31223116},
issn = {2050-084X},
abstract = {Modern microorganisms growing in fossils provide major challenges for researchers trying to detect ancient molecules in the same fossils.},
}

@article {pmid31220249,
year = {2019},
author = {Borówka, P and Pułaski, Ł and Marciniak, B and Borowska-Strugińska, B and Dziadek, J and Żądzińska, E and Lorkiewicz, W and Strapagiel, D},
title = {Screening methods for detection of ancient Mycobacterium tuberculosis complex fingerprints in next-generation sequencing data derived from skeletal samples.},
journal = {GigaScience},
volume = {8},
number = {6},
pages = {},
doi = {10.1093/gigascience/giz065},
pmid = {31220249},
issn = {2047-217X},
abstract = {BACKGROUND: Recent advances in ancient DNA studies, especially in increasing isolated DNA yields and quality, have opened the possibility of analysis of ancient host microbiome. However, such pitfalls as spurious identification of pathogens based on fragmentary data or environmental contamination could lead to incorrect epidaemiological conclusions. Within the Mycobacterium genus, Mycobacterium tuberculosis complex members responsible for tuberculosis share up to ∼99% genomic sequence identity, while other more distantly related Mycobacteria other than M. tuberculosis can be causative agents for pulmonary diseases or soil dwellers. Therefore, reliable determination of species complex is crucial for interpretation of sequencing results.

RESULTS: Here we present a novel bioinformatical approach, used for screening of ancient tuberculosis in sequencing data, derived from 28 individuals (dated 4400-4000 and 3100-2900 BC) from central Poland. We demonstrate that cost-effective next-generation screening sequencing data (∼20M reads per sample) could yield enough information to provide statistically supported identification of probable ancient disease cases.

CONCLUSIONS: Application of appropriate bioinformatic tools, including an unbiased selection of genomic alignment targets for species specificity, makes it possible to extract valid data from full-sample sequencing results (without subjective targeted enrichment procedures). This approach broadens the potential scope of palaeoepidaemiology both to older, suboptimally preserved samples and to pathogens with difficult intrageneric taxonomy.},
}

@article {pmid31216503,
year = {2019},
author = {Xavier, C and Eduardoff, M and Strobl, C and Parson, W},
title = {SD quants-Sensitive detection tetraplex-system for nuclear and mitochondrial DNA quantification and degradation inference.},
journal = {Forensic science international. Genetics},
volume = {42},
number = {},
pages = {39-44},
doi = {10.1016/j.fsigen.2019.06.004},
pmid = {31216503},
issn = {1878-0326},
abstract = {Measuring the quantity of DNA present in a forensic sample is relevant in a number of ways. First, it informs the analyst about the general DNA content to adjust the volume of DNA extract used for the genotyping assay to the optimal conditions (when possible). Second, quantification values can serve as plausibility checks for the performance of the DNA extraction method used as extraction positive and negative controls demand expected values. Third and relevant to highly compromised specimens, DNA quantification can inform about the degradation state of the DNA extracted from the unknown biological sample and aid the choice of downstream genotyping assays. While there are different, commercial products for the quantification of nuclear DNA available, commercial mitochondrial DNA (mtDNA) quantification systems are rare. Even more so, the simultaneous quantification of nuclear and mtDNA that is of relevance in highly degraded forensic specimens has rarely been described. We present here a novel real-time qPCR based tetraplex system termed SD quants that targets two different-sized mtDNA and a nuclear DNA region and includes an internal positive control to monitor potential inhibition. SD quants was compared to other existing quantification systems and subjected to analysis of severely degraded DNA present in ancient DNA and aged forensic specimens. This study complies with the MIQE (Bustin et al., 2009) guidelines (when applicable).},
}

@article {pmid31216315,
year = {2019},
author = {Wren, CD and Burke, A},
title = {Habitat suitability and the genetic structure of human populations during the Last Glacial Maximum (LGM) in Western Europe.},
journal = {PloS one},
volume = {14},
number = {6},
pages = {e0217996},
doi = {10.1371/journal.pone.0217996},
pmid = {31216315},
issn = {1932-6203},
abstract = {Human populations in Western Europe during the Last Glacial Maximum were geographically constrained to glacial refugia by the severity of the climate and ecological risk factors. In this research we use an agent-based model of human mobility and interaction, based on ethnographic and archaeological data, to explore the impact of ecological risk on human population structure via a reconstructed landscape of habitat suitability. The agent-based model allows us to evaluate the size and location of glacial refugia, the size of the populations occupying them and the degree of genetic relatedness between people occupying these areas. To do this, we model the probability of an agent foraging groups' survival as a function of habitat suitability. The model's simulated "genomes" (composed of regionally specific genetic markers) allow us to track long-term trends of inter-regional interaction and mobility. The results agree with previous archaeological studies situating a large glacial refugium spanning southern France and northeastern Spain, but we expand on those studies by demonstrating that higher rates of population growth in this central refugium led to continuous out-migration and therefore genetic homogeneity across Western Europe, with the possible exception of the Italian peninsula. These results concur with material culture data from known archaeological sites dating to the Last Glacial Maximum and make predictions for future ancient DNA studies.},
}

@article {pmid31216119,
year = {2019},
author = {Epp, LS},
title = {A global perspective for biodiversity history with ancient environmental DNA.},
journal = {Molecular ecology},
volume = {28},
number = {10},
pages = {2456-2458},
doi = {10.1111/mec.15118},
pmid = {31216119},
issn = {1365-294X},
support = {98/3-1//DFG/ ; //Belmont Forum/ ; //BiodivScen ERA-Net COFUND programme/ ; },
abstract = {The past centuries have seen tremendous turnovers in species distributions and biodiversity due to anthropogenic impacts on a global scale. The processes are ongoing and mostly not well documented. Long-term records of biotic change can be recovered from sedimentary deposits, but traditional analyses were restricted to organisms that leave behind visible traces and molecular genetic tools were mostly employed on samples that promised good DNA preservation. In this issue of Molecular Ecology, Shaw, Weyrich, Hallegraeff and Cooper (2019) and Gomez Cabrera et al. (2019) present two studies on marine sedimentary records from warm environments, in which they successfully analyze ancient environmental DNA (aeDNA) on a decadal and centennial scale. Notably, the studies were conducted on novel samples with nonoptimal preservation conditions for ancient DNA - historical collections of ship ballast tank sediments from Australia and two coral reef cores spanning up to 750 years (Figure 1) - but yielded a high diversity of taxa. This highlights that aeDNA is a promising tool to globally study biodiversity history on scales of decades to centuries - the timeframe most relevant to human society in the context of both current climate change and direct anthropogenic modifications of the environment.},
}

@article {pmid31209287,
year = {2019},
author = {Bradshaw, CJA and Ulm, S and Williams, AN and Bird, MI and Roberts, RG and Jacobs, Z and Laviano, F and Weyrich, LS and Friedrich, T and Norman, K and Saltré, F},
title = {Minimum founding populations for the first peopling of Sahul.},
journal = {Nature ecology & evolution},
volume = {3},
number = {7},
pages = {1057-1063},
doi = {10.1038/s41559-019-0902-6},
pmid = {31209287},
issn = {2397-334X},
abstract = {The timing, context and nature of the first people to enter Sahul is still poorly understood owing to a fragmented archaeological record. However, quantifying the plausible demographic context of this founding population is essential to determine how and why the initial peopling of Sahul occurred. We developed a stochastic, age-structured model using demographic rates from hunter-gatherer societies, and relative carrying capacity hindcasted with LOVECLIM's net primary productivity for northern Sahul. We projected these populations to determine the resilience and minimum sizes required to avoid extinction. A census founding population of between 1,300 and 1,550 individuals was necessary to maintain a quasi-extinction threshold of ≲0.1. This minimum founding population could have arrived at a single point in time, or through multiple voyages of ≥130 people over ~700-900 years. This result shows that substantial population amalgamation in Sunda and Wallacea in Marine Isotope Stages 3-4 provided the conditions for the successful, large-scale and probably planned peopling of Sahul.},
}

@article {pmid31184205,
year = {2019},
author = {Scheib, CL and Hui, R and D'Atanasio, E and Wohns, AW and Inskip, SA and Rose, A and Cessford, C and O'Connell, TC and Robb, JE and Evans, C and Patten, R and Kivisild, T},
title = {East Anglian early Neolithic monument burial linked to contemporary Megaliths.},
journal = {Annals of human biology},
volume = {46},
number = {2},
pages = {145-149},
doi = {10.1080/03014460.2019.1623912},
pmid = {31184205},
issn = {1464-5033},
abstract = {In the fourth millennium BCE a cultural phenomenon of monumental burial structures spread along the Atlantic façade. Megalithic burials have been targeted for aDNA analyses, but a gap remains in East Anglia, where Neolithic structures were generally earthen or timber. An early Neolithic (3762-3648 cal. BCE) burial monument at the site of Trumpington Meadows, Cambridgeshire, UK, contained the partially articulated remains of at least three individuals. To determine whether this monument fits a pattern present in megalithic burials regarding sex bias, kinship, diet and relationship to modern populations, teeth and ribs were analysed for DNA and carbon and nitrogen isotopic values, respectively. Whole ancient genomes were sequenced from two individuals to a mean genomic coverage of 1.6 and 1.2X and genotypes imputed. Results show that they were brothers from a small population genetically and isotopically similar to previously published British Neolithic individuals, with a level of genome-wide homozygosity consistent with a small island population sourced from continental Europe, but bearing no signs of recent inbreeding. The first Neolithic whole genomes from a monumental burial in East Anglia confirm that this region was connected with the larger pattern of Neolithic megaliths in the British Isles and the Atlantic façade.},
}

@article {pmid31178321,
year = {2019},
author = {Delsuc, F and Kuch, M and Gibb, GC and Karpinski, E and Hackenberger, D and Szpak, P and Martínez, JG and Mead, JI and McDonald, HG and MacPhee, RDE and Billet, G and Hautier, L and Poinar, HN},
title = {Ancient Mitogenomes Reveal the Evolutionary History and Biogeography of Sloths.},
journal = {Current biology : CB},
volume = {29},
number = {12},
pages = {2031-2042.e6},
doi = {10.1016/j.cub.2019.05.043},
pmid = {31178321},
issn = {1879-0445},
abstract = {Living sloths represent two distinct lineages of small-sized mammals that independently evolved arboreality from terrestrial ancestors. The six extant species are the survivors of an evolutionary radiation marked by the extinction of large terrestrial forms at the end of the Quaternary. Until now, sloth evolutionary history has mainly been reconstructed from phylogenetic analyses of morphological characters. Here, we used ancient DNA methods to successfully sequence 10 extinct sloth mitogenomes encompassing all major lineages. This includes the iconic continental ground sloths Megatherium, Megalonyx, Mylodon, and Nothrotheriops and the smaller endemic Caribbean sloths Parocnus and Acratocnus. Phylogenetic analyses identify eight distinct lineages grouped in three well-supported clades, whose interrelationships are markedly incongruent with the currently accepted morphological topology. We show that recently extinct Caribbean sloths have a single origin but comprise two highly divergent lineages that are not directly related to living two-fingered sloths, which instead group with Mylodon. Moreover, living three-fingered sloths do not represent the sister group to all other sloths but are nested within a clade of extinct ground sloths including Megatherium, Megalonyx, and Nothrotheriops. Molecular dating also reveals that the eight newly recognized sloth families all originated between 36 and 28 million years ago (mya). The early divergence of recently extinct Caribbean sloths around 35 mya is consistent with the debated GAARlandia hypothesis postulating the existence at that time of a biogeographic connection between northern South America and the Greater Antilles. This new molecular phylogeny has major implications for reinterpreting sloth morphological evolution, biogeography, and diversification history.},
}

@article {pmid31171860,
year = {2019},
author = {Presslee, S and Slater, GJ and Pujos, F and Forasiepi, AM and Fischer, R and Molloy, K and Mackie, M and Olsen, JV and Kramarz, A and Taglioretti, M and Scaglia, F and Lezcano, M and Lanata, JL and Southon, J and Feranec, R and Bloch, J and Hajduk, A and Martin, FM and Salas Gismondi, R and Reguero, M and de Muizon, C and Greenwood, A and Chait, BT and Penkman, K and Collins, M and MacPhee, RDE},
title = {Palaeoproteomics resolves sloth relationships.},
journal = {Nature ecology & evolution},
volume = {3},
number = {7},
pages = {1121-1130},
doi = {10.1038/s41559-019-0909-z},
pmid = {31171860},
issn = {2397-334X},
abstract = {The living tree sloths Choloepus and Bradypus are the only remaining members of Folivora, a major xenarthran radiation that occupied a wide range of habitats in many parts of the western hemisphere during the Cenozoic, including both continents and the West Indies. Ancient DNA evidence has played only a minor role in folivoran systematics, as most sloths lived in places not conducive to genomic preservation. Here we utilize collagen sequence information, both separately and in combination with published mitochondrial DNA evidence, to assess the relationships of tree sloths and their extinct relatives. Results from phylogenetic analysis of these datasets differ substantially from morphology-based concepts: Choloepus groups with Mylodontidae, not Megalonychidae; Bradypus and Megalonyx pair together as megatherioids, while monophyletic Antillean sloths may be sister to all other folivorans. Divergence estimates are consistent with fossil evidence for mid-Cenozoic presence of sloths in the West Indies and an early Miocene radiation in South America.},
}

@article {pmid31164419,
year = {2019},
author = {Keller, M and Spyrou, MA and Scheib, CL and Neumann, GU and Kröpelin, A and Haas-Gebhard, B and Päffgen, B and Haberstroh, J and Ribera I Lacomba, A and Raynaud, C and Cessford, C and Durand, R and Stadler, P and Nägele, K and Bates, JS and Trautmann, B and Inskip, SA and Peters, J and Robb, JE and Kivisild, T and Castex, D and McCormick, M and Bos, KI and Harbeck, M and Herbig, A and Krause, J},
title = {Ancient Yersinia pestis genomes from across Western Europe reveal early diversification during the First Pandemic (541-750).},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {116},
number = {25},
pages = {12363-12372},
doi = {10.1073/pnas.1820447116},
pmid = {31164419},
issn = {1091-6490},
support = {//Wellcome Trust/United Kingdom ; },
abstract = {The first historically documented pandemic caused by Yersinia pestis began as the Justinianic Plague in 541 within the Roman Empire and continued as the so-called First Pandemic until 750. Although paleogenomic studies have previously identified the causative agent as Y. pestis, little is known about the bacterium's spread, diversity, and genetic history over the course of the pandemic. To elucidate the microevolution of the bacterium during this time period, we screened human remains from 21 sites in Austria, Britain, Germany, France, and Spain for Y. pestis DNA and reconstructed eight genomes. We present a methodological approach assessing single-nucleotide polymorphisms (SNPs) in ancient bacterial genomes, facilitating qualitative analyses of low coverage genomes from a metagenomic background. Phylogenetic analysis on the eight reconstructed genomes reveals the existence of previously undocumented Y. pestis diversity during the sixth to eighth centuries, and provides evidence for the presence of multiple distinct Y. pestis strains in Europe. We offer genetic evidence for the presence of the Justinianic Plague in the British Isles, previously only hypothesized from ambiguous documentary accounts, as well as the parallel occurrence of multiple derived strains in central and southern France, Spain, and southern Germany. Four of the reported strains form a polytomy similar to others seen across the Y. pestis phylogeny, associated with the Second and Third Pandemics. We identified a deletion of a 45-kb genomic region in the most recent First Pandemic strains affecting two virulence factors, intriguingly overlapping with a deletion found in 17th- to 18th-century genomes of the Second Pandemic.},
}

@article {pmid31163991,
year = {2019},
author = {Santander, C and Montinaro, F and Capelli, C},
title = {Searching for archaic contribution in Africa.},
journal = {Annals of human biology},
volume = {46},
number = {2},
pages = {129-139},
doi = {10.1080/03014460.2019.1624823},
pmid = {31163991},
issn = {1464-5033},
abstract = {Context: Africa's role in the narrative of human evolution is indisputably emphasised in the emergence of Homo sapiens. However, once humans dispersed beyond Africa, the history of those who stayed remains vastly under-studied, lacking the proper attention the birthplace of both modern and archaic humans deserves. The sequencing of Neanderthal and Denisovan genomes has elucidated evidence of admixture between archaic and modern humans outside of Africa, but has not aided efforts in answering whether archaic admixture happened within Africa. Objectives: This article reviews the state of research for archaic introgression in African populations and discusses recent insights into this topic. Methods: Gathering published sources and recently released preprints, this review reports on the different methods developed for detecting archaic introgression. Particularly it discusses how relevant these are when implemented on African populations and what findings these studies have shown so far. Results: Methods for detecting archaic introgression have been predominantly developed and implemented on non-African populations. Recent preprints present new methods considering African populations. While a number of studies using these methods suggest archaic introgression in Africa, without an African archaic genome to validate these results, such findings remain as putative archaic introgression. Conclusion: In light of the caveats with implementing current archaic introgression detection methods in Africa, we recommend future studies to concentrate on unravelling the complicated demographic history of Africa through means of ancient DNA where possible and through more focused efforts to sequence modern DNA from more representative populations across the African continent.},
}

@article {pmid31161006,
year = {2019},
author = {Ersmark, E and Baryshnikov, G and Higham, T and Argant, A and Castaños, P and Döppes, D and Gasparik, M and Germonpré, M and Lidén, K and Lipecki, G and Marciszak, A and Miller, R and Moreno-García, M and Pacher, M and Robu, M and Rodriguez-Varela, R and Rojo Guerra, M and Sabol, M and Spassov, N and Storå, J and Valdiosera, C and Villaluenga, A and Stewart, JR and Dalén, L},
title = {Genetic turnovers and northern survival during the last glacial maximum in European brown bears.},
journal = {Ecology and evolution},
volume = {9},
number = {10},
pages = {5891-5905},
doi = {10.1002/ece3.5172},
pmid = {31161006},
issn = {2045-7758},
abstract = {The current phylogeographic pattern of European brown bears (Ursus arctos) has commonly been explained by postglacial recolonization out of geographically distinct refugia in southern Europe, a pattern well in accordance with the expansion/contraction model. Studies of ancient DNA from brown bear remains have questioned this pattern, but have failed to explain the glacial distribution of mitochondrial brown bear clades and their subsequent expansion across the European continent. We here present 136 new mitochondrial sequences generated from 346 remains from Europe, ranging in age between the Late Pleistocene and historical times. The genetic data show a high Late Pleistocene diversity across the continent and challenge the strict confinement of bears to traditional southern refugia during the last glacial maximum (LGM). The mitochondrial data further suggest a genetic turnover just before this time, as well as a steep demographic decline starting in the mid-Holocene. Levels of stable nitrogen isotopes from the remains confirm a previously proposed shift toward increasing herbivory around the LGM in Europe. Overall, these results suggest that in addition to climate, anthropogenic impact and inter-specific competition may have had more important effects on the brown bear's ecology, demography, and genetic structure than previously thought.},
}

@article {pmid31147405,
year = {2019},
author = {Prendergast, ME and Lipson, M and Sawchuk, EA and Olalde, I and Ogola, CA and Rohland, N and Sirak, KA and Adamski, N and Bernardos, R and Broomandkhoshbacht, N and Callan, K and Culleton, BJ and Eccles, L and Harper, TK and Lawson, AM and Mah, M and Oppenheimer, J and Stewardson, K and Zalzala, F and Ambrose, SH and Ayodo, G and Gates, HL and Gidna, AO and Katongo, M and Kwekason, A and Mabulla, AZP and Mudenda, GS and Ndiema, EK and Nelson, C and Robertshaw, P and Kennett, DJ and Manthi, FK and Reich, D},
title = {Ancient DNA reveals a multistep spread of the first herders into sub-Saharan Africa.},
journal = {Science (New York, N.Y.)},
volume = {365},
number = {6448},
pages = {},
doi = {10.1126/science.aaw6275},
pmid = {31147405},
issn = {1095-9203},
support = {R01 GM100233/GM/NIGMS NIH HHS/United States ; /HHMI/Howard Hughes Medical Institute/United States ; },
abstract = {How food production first entered eastern Africa ~5000 years ago and the extent to which people moved with livestock is unclear. We present genome-wide data from 41 individuals associated with Later Stone Age, Pastoral Neolithic (PN), and Iron Age contexts in what are now Kenya and Tanzania to examine the genetic impacts of the spreads of herding and farming. Our results support a multiphase model in which admixture between northeastern African-related peoples and eastern African foragers formed multiple pastoralist groups, including a genetically homogeneous PN cluster. Additional admixture with northeastern and western African-related groups occurred by the Iron Age. These findings support several movements of food producers while rejecting models of minimal admixture with foragers and of genetic differentiation between makers of distinct PN artifacts.},
}

@article {pmid31137975,
year = {2019},
author = {Donoghue, HD},
title = {Tuberculosis and leprosy associated with historical human population movements in Europe and beyond - an overview based on mycobacterial ancient DNA.},
journal = {Annals of human biology},
volume = {46},
number = {2},
pages = {120-128},
doi = {10.1080/03014460.2019.1624822},
pmid = {31137975},
issn = {1464-5033},
abstract = {Context: Tuberculosis and leprosy are readily recognised in human remains due to their typical palaeopathology. Both Mycobacterium tuberculosis (MTB) and Mycobacterium leprae (ML) are obligate pathogens and have been detected in ancient human populations. Objective: To demonstrate historical tuberculosis and leprosy cases in Europe and beyond using molecular methods, as human populations are associated with different mycobacterial genotypes. Methods: MTB and ML ancient DNA (aDNA) has been detected by DNA amplification using PCR, or by whole genome sequencing. Mycobacterial cell wall lipids also provide specific markers for identification. Results: In 18th century Hungary, the European indigenous MTB genotype 4 strains have been found. However, many individuals were co-infected with up to three MTB sub-genotypes. In 8th-14th century Europe significant differences in ML genotypes were found between northwest Europe compared with central, southern, or eastern Europe. In addition, several co-infections of MTB and ML were detected in historical samples. Conclusion: Both MTB and ML strain types differ between geographically separate populations. This is associated with ancient human migration after an evolutionary bottleneck and clonal expansion. The absence of indigenous leprosy in Europe today may be due to the greater mortality of tuberculosis in individuals who are co-infected with both organisms.},
}

@article {pmid31134682,
year = {2019},
author = {Wu, X and Ding, B and Zhang, B and Feng, J and Wang, Y and Ning, C and Wu, H and Zhang, F and Zhang, Q and Li, N and Zhang, Z and Sun, X and Zhang, Q and Li, W and Liu, B and Cui, Y and Gong, L},
title = {Phylogenetic and population structural inference from genomic ancestry maintained in present-day common wheat Chinese landraces.},
journal = {The Plant journal : for cell and molecular biology},
volume = {99},
number = {2},
pages = {201-215},
doi = {10.1111/tpj.14421},
pmid = {31134682},
issn = {1365-313X},
support = {2016YFD0101004//National key research and development programme of China/ ; 41472024//National Natural Science Foundation of China/ ; 2412017BJ005//Fundamental Research Fund for the Central Universities/ ; //Recruitment Programme of Global Youth Experts/ ; //Programme of Changbai Mountain Scholars/ ; },
abstract = {Hexaploid common wheat is one of the most important food crops worldwide. Common wheat domestication began in the Fertile Crescent of the Near East approximately 10 000 years ago and then spread west into Europe and eastward into East Asia and China. However, the possible spreading route into and within China is still unclear. In this study, we successfully extracted DNA from single ancient wheat seeds and sequenced the whole genome of seven ancient samples from Xiaohe and Gumugou cemeteries in Xinjiang, China. Genomic inference and morphological observation confirmed their identity as hexaploid common wheat grown in prehistoric China at least 3200 years before present (BP). Phylogenetic and admixture analyses with RNA-seq data of modern hexaploid wheat cultivars from both China and Western countries demonstrated a close kinship of the ancient wheat to extant common wheat landraces in southwestern China. The highly similar allelic frequencies in modern landraces of the Qinghai-Tibetan plateau with the ancient wheat support the previously suggested southwestern spreading route into highland China. A subsequent dispersal route from the Qinghai-Tibetan plateau margins to the Yangtze valley was proposed in this study. Furthermore, the common wheat populations grown in the Middle and Lower Yangtze valley wheat zones were also proposed to be established by population admixture with the wheat grown in the Upper Yangtze valley. Our study reports ancient common wheat sequences at a genome-wide scale, providing important information on the origin, dispersal, and genetic improvement under cultivation of present-day wheat landraces grown in China.},
}

@article {pmid31124326,
year = {2019},
author = {Hong, JH and Oh, CS and Chai, JY and Seo, M and Shin, DH},
title = {Cytochrome C Oxidase Subunit 1, Internal Transcribed Spacer 1, Nicotinamide Adenine Dinucleotide Hydrogen Dehydrogenase Subunits 2 and 5 of Clonorchis sinensis Ancient DNA Retrieved from Joseon Dynasty Mummy Specimens.},
journal = {Journal of Korean medical science},
volume = {34},
number = {20},
pages = {e149},
doi = {10.3346/jkms.2019.34.e149},
pmid = {31124326},
issn = {1598-6357},
support = {NRF-2016R1A2B4015669/NRF/National Research Foundation of Korea/Korea ; },
abstract = {We analyzed Clonorchis sinensis ancient DNA (aDNA) acquired from the specimens of the Joseon mummies. The target regions were cytochrome C oxidase subunit 1 (CO1), internal transcribed spacer 1 (ITS1), nicotinamide adenine dinucleotide hydrogen (NADH) dehydrogenase subunits 2 (NAD2) and 5 (NAD5). The sequences of C. sinensis aDNA was completely or almost identical to modern C. sinensis sequences in GenBank. We also found that ITS1, NAD2 and NAD5 could be good markers for molecular diagnosis between C. sinensis and the other trematode parasite species. The current result could improve our knowledge about genetic history of C. sinensis.},
}

@article {pmid31123709,
year = {2019},
author = {Kashuba, N and Kırdök, E and Damlien, H and Manninen, MA and Nordqvist, B and Persson, P and Götherström, A},
title = {Ancient DNA from mastics solidifies connection between material culture and genetics of mesolithic hunter-gatherers in Scandinavia.},
journal = {Communications biology},
volume = {2},
number = {},
pages = {185},
doi = {10.1038/s42003-019-0399-1},
pmid = {31123709},
issn = {2399-3642},
abstract = {Human demography research in grounded on the information derived from ancient DNA and archaeology. For example, the study on the early postglacial dual-route colonisation of the Scandinavian Peninsula is largely based on associating genomic data with the early dispersal of lithic technology from the East European Plain. However, a clear connection between material culture and genetics has been lacking. Here, we demonstrate that direct connection by analysing human DNA from chewed birch bark pitch mastics. These samples were discovered at Huseby Klev in western Sweden, a Mesolithic site with eastern lithic technology. We generated genome-wide data for three individuals, and show their affinity to the Scandinavian hunter-gatherers. Our samples date to 9880-9540 calBP, expanding the temporal range and distribution of the early Scandinavian genetic group. We propose that DNA from ancient mastics can be used to study environment and ecology of prehistoric populations.},
}

@article {pmid31116227,
year = {2019},
author = {Hong, JH and Oh, CS and Seo, M and Shin, DH},
title = {Analysis of COI and ITS2 regions of DNA obtained from Paragonimus westermani eggs in ancient coprolites on Joseon dynasty mummies.},
journal = {Memorias do Instituto Oswaldo Cruz},
volume = {114},
number = {},
pages = {e180595},
doi = {10.1590/0074-02760180595},
pmid = {31116227},
issn = {1678-8060},
mesh = {Animals ; Asia ; DNA, Helminth/*genetics ; DNA, Ribosomal Spacer/*genetics ; Electron Transport Complex IV/*genetics ; Feces/*parasitology ; Humans ; Mummies/*parasitology ; Paleodontology ; Paragonimus westermani/genetics/*isolation & purification ; Parasite Egg Count ; Phylogeny ; },
abstract = {The genetic information of ancient Paragonimus westermani, the oriental lung fluke infecting over 20 million people worldwide, has not been thoroughly investigated thus far. We analysed genetic markers (COI and ITS2) of P. westermani from coprolite specimens (n = 6) obtained from 15th to 18th century Korean mummies. Our results indicated that all P. westermani sequences were generally distinct from the other species of the genus Paragonimus. The sequences were clustered into three groups: Group I for East Asia; Group II for South and Southeast Asia; and Group III for India and Sri Lanka. In this study, we found that ancient P. westermani sequences in Korea belong to Group I, adding invaluable information to the existing knowledge of Paragonimus paleogenetics.},
}

Animals
Asia
DNA, Helminth/*genetics
DNA, Ribosomal Spacer/*genetics
Electron Transport Complex IV/*genetics
Feces/*parasitology
Humans
Mummies/*parasitology
Paleodontology
Paragonimus westermani/genetics/*isolation & purification
Parasite Egg Count
Phylogeny

@article {pmid31113254,
year = {2019},
author = {Ham, E and Underdown, SJ and Houldcroft, CJ},
title = {The relative roles of maternal survival and inter-personal violence as selection pressures on the persistence of Neanderthal hypercoagulability alleles in modern Europeans.},
journal = {Annals of human biology},
volume = {46},
number = {2},
pages = {99-108},
doi = {10.1080/03014460.2019.1622038},
pmid = {31113254},
issn = {1464-5033},
abstract = {Background: Simonti et al. reported variation in the frequency of Neanderthal alleles found in modern humans and argued that they may have provided an evolutionary advantage. One such allele is SNP rs3917862, associated with hypercoagulability. rs3917862 can be deleterious, but can also help prevent blood loss. Aim: To investigate two possible selective pressure hypotheses for rs3917862 surviving to higher frequencies: deaths from interpersonal violent trauma and childbirth. Subjects and methods: Mortality data from modern hunter-gatherers models the living conditions and causes of death of humans and Neanderthals at the point of admixture. Results: National census data indicates a positive correlation between the presence of rs3917862 and decreased maternal mortality ratios. When the maternal mortality ratio is modelled using GDP, births attended by skilled assistants and the presence of rs3917862, women are 0.1% more likely to die in childbirth in populations lacking rs3917862. Deaths due to violence show no correlation with rs3917862. Conclusion: These findings challenge the idea that Neanderthal admixture has negatively impacted the overall health of modern humans. Maternal survival may have acted as a selective pressure for the persistence of hypercoagulability alleles in modern Europeans. Understanding the role of hypercoagulability in childbirth, and the role of rs3917862, could help to reduce maternal mortality ratios.},
}

@article {pmid31109769,
year = {2019},
author = {Perry, GH and Makarewicz, CA},
title = {Horse Paleogenomes and Human-Animal Interactions in Prehistory.},
journal = {Trends in genetics : TIG},
volume = {35},
number = {7},
pages = {473-475},
doi = {10.1016/j.tig.2019.04.006},
pmid = {31109769},
issn = {0168-9525},
abstract = {A new analysis of paleogenomic data from 278 ancient horses (Fages et al. Cellhttp://doi.org/10.1016/j.cell.2019.03.049) finds that this animal - crucially important to many ancient and contemporary human societies for subsistence, transportation, conflict, and more - was domesticated in at least two different regions, but with the geographic and cultural origins of the modern domestic horse lineage remaining unknown. By tracing ancient horse population movements and inferring the spatiotemporal trajectories of phenotypic adaptations, this study provides fresh perspectives on past human group interactions and activities.},
}

@article {pmid31092597,
year = {2019},
author = {Bennett, RJ and Baker, KS},
title = {Looking Backward To Move Forward: the Utility of Sequencing Historical Bacterial Genomes.},
journal = {Journal of clinical microbiology},
volume = {57},
number = {8},
pages = {},
doi = {10.1128/JCM.00100-19},
pmid = {31092597},
issn = {1098-660X},
abstract = {Many pathogens that caused devastating disease throughout human history, such as Yersinia pestis, Mycobacterium tuberculosis, and Mycobacterium leprae, remain problematic today. Historical bacterial genomes represent a unique source of genetic information and advancements in sequencing technologies have allowed unprecedented insights from this previously understudied resource. This minireview brings together example studies which have utilized ancient DNA, individual historical isolates (both extant and dead) and collections of historical isolates. The studies span human history and highlight the contribution that sequencing and analysis of historical bacterial genomes have made to a wide variety of fields. From providing retrospective diagnosis, to uncovering epidemiological pathways and characterizing genetic diversity, there is clear evidence for the utility of historical isolate studies in understanding disease today. Studies utilizing historical isolate collections, such as those from the National Collection of Type Cultures, the American Type Culture Collection, and the Institut Pasteur, offer enhanced insight since they typically span a wide time period encompassing important historical events and are useful for the investigating the phylodynamics of pathogens. Furthermore, historical sequencing studies are particularly useful for looking into the evolution of antimicrobial resistance, a major public health concern. In summary, although there are limitations to working with historical bacterial isolates, especially when utilizing ancient DNA, continued improvement in molecular and sequencing technologies and the resourcefulness of investigators mean this area of study will continue to expand and contribute to the understanding of pathogens.},
}

@article {pmid31088861,
year = {2019},
author = {Renaud, G and Hanghøj, K and Korneliussen, TS and Willerslev, E and Orlando, L},
title = {Joint Estimates of Heterozygosity and Runs of Homozygosity for Modern and Ancient Samples.},
journal = {Genetics},
volume = {212},
number = {3},
pages = {587-614},
doi = {10.1534/genetics.119.302057},
pmid = {31088861},
issn = {1943-2631},
abstract = {Both the total amount and the distribution of heterozygous sites within individual genomes are informative about the genetic diversity of the population they belong to. Detecting true heterozygous sites in ancient genomes is complicated by the generally limited coverage achieved and the presence of post-mortem damage inflating sequencing errors. Additionally, large runs of homozygosity found in the genomes of particularly inbred individuals and of domestic animals can skew estimates of genome-wide heterozygosity rates. Current computational tools aimed at estimating runs of homozygosity and genome-wide heterozygosity levels are generally sensitive to such limitations. Here, we introduce ROHan, a probabilistic method which substantially improves the estimate of heterozygosity rates both genome-wide and for genomic local windows. It combines a local Bayesian model and a Hidden Markov Model at the genome-wide level and can work both on modern and ancient samples. We show that our algorithm outperforms currently available methods for predicting heterozygosity rates for ancient samples. Specifically, ROHan can delineate large runs of homozygosity (at megabase scales) and produce a reliable confidence interval for the genome-wide rate of heterozygosity outside of such regions from modern genomes with a depth of coverage as low as 5-6× and down to 7-8× for ancient samples showing moderate DNA damage. We apply ROHan to a series of modern and ancient genomes previously published and revise available estimates of heterozygosity for humans, chimpanzees and horses.},
}

@article {pmid31080507,
year = {2019},
author = {Upadhyay, M and Bortoluzzi, C and Barbato, M and Ajmone-Marsan, P and Colli, L and Ginja, C and Sonstegard, TS and Bosse, M and Lenstra, JA and Groenen, MAM and Crooijmans, RPMA},
title = {Deciphering the patterns of genetic admixture and diversity in southern European cattle using genome-wide SNPs.},
journal = {Evolutionary applications},
volume = {12},
number = {5},
pages = {951-963},
doi = {10.1111/eva.12770},
pmid = {31080507},
issn = {1752-4571},
abstract = {The divergence between indicine cattle (Bos indicus) and taurine cattle (Bos taurus) is estimated to have occurred approximately 250,000 years ago, but a small number of European cattle breeds still display shared ancestry with indicine cattle. Additionally, following the divergence of African and European taurine, the gene flow between African taurine and southern European cattle has also been proposed. However, the extent to which non-European cattle ancestry is diffused across southern European cattle has not been investigated thoroughly. Also, in recent times, many local breeds have suffered severe reductions in effective population size. Therefore, in the present study, we investigated the pattern of genetic diversity in various European cattle based on single nucleotide polymorphisms (SNP) identified from whole-genome sequencing data. Additionally, we also employed unlinked and phased SNP-based approaches on high-density SNP array data to characterize non-European cattle ancestry in several southern European cattle breeds. Using heterozygosity-based parameters, we concluded that, on average, nucleotide diversity is greater in southern European cattle than western European (British and commercial) cattle. However, an abundance of long runs of homozygosity (ROH) and the pattern of Linkage disequilibrium decay suggested recent bottlenecks in Maltese and Romagnola. High nucleotide diversity outside ROH indicated a highly diverse founder population for southern European and African taurine. We also show that Iberian cattle display shared ancestry with African cattle. Furthermore, we show that Podolica is an ancient cross-bred between Indicine zebu and European taurine. Additionally, we also inferred similar ancestry profile of non-European cattle ancestry in different Balkan and Italian cattle breeds which might be an indication of the common origin of indicine ancestry in these breeds. Finally, we discuss several plausible demographic scenarios which might account for the presence of non-European cattle ancestry in these cattle breeds.},
}

@article {pmid31080083,
year = {2019},
author = {Saag, L and Laneman, M and Varul, L and Malve, M and Valk, H and Razzak, MA and Shirobokov, IG and Khartanovich, VI and Mikhaylova, ER and Kushniarevich, A and Scheib, CL and Solnik, A and Reisberg, T and Parik, J and Saag, L and Metspalu, E and Rootsi, S and Montinaro, F and Remm, M and Mägi, R and D'Atanasio, E and Crema, ER and Díez-Del-Molino, D and Thomas, MG and Kriiska, A and Kivisild, T and Villems, R and Lang, V and Metspalu, M and Tambets, K},
title = {The Arrival of Siberian Ancestry Connecting the Eastern Baltic to Uralic Speakers further East.},
journal = {Current biology : CB},
volume = {29},
number = {10},
pages = {1701-1711.e16},
doi = {10.1016/j.cub.2019.04.026},
pmid = {31080083},
issn = {1879-0445},
support = {261213//European Research Council/International ; },
abstract = {In this study, we compare the genetic ancestry of individuals from two as yet genetically unstudied cultural traditions in Estonia in the context of available modern and ancient datasets: 15 from the Late Bronze Age stone-cist graves (1200-400 BC) (EstBA) and 6 from the Pre-Roman Iron Age tarand cemeteries (800/500 BC-50 AD) (EstIA). We also included 5 Pre-Roman to Roman Iron Age Ingrian (500 BC-450 AD) (IngIA) and 7 Middle Age Estonian (1200-1600 AD) (EstMA) individuals to build a dataset for studying the demographic history of the northern parts of the Eastern Baltic from the earliest layer of Mesolithic to modern times. Our findings are consistent with EstBA receiving gene flow from regions with strong Western hunter-gatherer (WHG) affinities and EstIA from populations related to modern Siberians. The latter inference is in accordance with Y chromosome (chrY) distributions in present day populations of the Eastern Baltic, as well as patterns of autosomal variation in the majority of the westernmost Uralic speakers [1-5]. This ancestry reached the coasts of the Baltic Sea no later than the mid-first millennium BC; i.e., in the same time window as the diversification of west Uralic (Finnic) languages [6]. Furthermore, phenotypic traits often associated with modern Northern Europeans, like light eyes, hair, and skin, as well as lactose tolerance, can be traced back to the Bronze Age in the Eastern Baltic. VIDEO ABSTRACT.},
}

@article {pmid31075917,
year = {2019},
author = {Merheb, M and Matar, R and Hodeify, R and Siddiqui, SS and Vazhappilly, CG and Marton, J and Azharuddin, S and Al Zouabi, H},
title = {Mitochondrial DNA, a Powerful Tool to Decipher Ancient Human Civilization from Domestication to Music, and to Uncover Historical Murder Cases.},
journal = {Cells},
volume = {8},
number = {5},
pages = {},
doi = {10.3390/cells8050433},
pmid = {31075917},
issn = {2073-4409},
abstract = {Mitochondria are unique organelles carrying their own genetic material, independent from that in the nucleus. This review will discuss the nature of mitochondrial DNA (mtDNA) and its levels in the cell, which are the key elements to consider when trying to achieve molecular identification in ancient and degraded samples. mtDNA sequence analysis has been appropriately validated and is a consistent molecular target for the examination of biological evidence encountered in forensic cases-and profiling, in certain conditions-especially for burnt bodies and degraded samples of all types. Exceptional cases and samples will be discussed in this review, such as mtDNA from leather in Beethoven's grand piano, mtDNA in mummies, and solving famous historical criminal cases. In addition, this review will be discussing the use of ancient mtDNA to understand past human diet, to trace historical civilizations and ancient trade routes, and to uncover geographical domestication origins and lineage relationships. In each topic, we will present the power of mtDNA and how, in many cases, no nuclear DNA was left, leaving mitochondrial DNA analysis as a powerful alternative. Exploring this powerful tool further will be extremely useful to modern science and researchers, due to its capabilities in providing us with previously unattainable knowledge.},
}

@article {pmid31074010,
year = {2019},
author = {Maurer-Alcalá, XX and Nowacki, M},
title = {Evolutionary origins and impacts of genome architecture in ciliates.},
journal = {Annals of the New York Academy of Sciences},
volume = {1447},
number = {1},
pages = {110-118},
doi = {10.1111/nyas.14108},
pmid = {31074010},
issn = {1749-6632},
support = {//NCCR RNA & Disease/ ; G-EDIT//H2020 European Research Council/ ; },
abstract = {Genome architecture is well diversified among eukaryotes in terms of size and content, with many being radically shaped by ancient and ongoing genome conflicts with transposable elements (e.g., the large transposon-rich genomes common among plants). In ciliates, a group of microbial eukaryotes with distinct somatic and germ-line genomes present in a single cell, the consequences of these genome conflicts are most apparent in their developmentally programmed genome rearrangements. This complicated developmental phenomenon has largely overshadowed and outpaced our understanding of how germ-line and somatic genome architectures have influenced the evolutionary dynamism and potential in these taxa. In our review, we highlight three central concepts: how the evolution of atypical ciliate germ-line genome architectures is linked to ancient genome conflicts; how the complex, epigenetically guided transformation of germline to soma during development can generate widespread genetic variation; and how these features, coupled with their unusual life cycle, have increased the rate of molecular evolution linked to genome architecture in these taxa.},
}

@article {pmid31061125,
year = {2019},
author = {Schroeder, H and Margaryan, A and Szmyt, M and Theulot, B and Włodarczak, P and Rasmussen, S and Gopalakrishnan, S and Szczepanek, A and Konopka, T and Jensen, TZT and Witkowska, B and Wilk, S and Przybyła, MM and Pospieszny, Ł and Sjögren, KG and Belka, Z and Olsen, J and Kristiansen, K and Willerslev, E and Frei, KM and Sikora, M and Johannsen, NN and Allentoft, ME},
title = {Unraveling ancestry, kinship, and violence in a Late Neolithic mass grave.},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {116},
number = {22},
pages = {10705-10710},
doi = {10.1073/pnas.1820210116},
pmid = {31061125},
issn = {1091-6490},
abstract = {The third millennium BCE was a period of major cultural and demographic changes in Europe that signaled the beginning of the Bronze Age. People from the Pontic steppe expanded westward, leading to the formation of the Corded Ware complex and transforming the genetic landscape of Europe. At the time, the Globular Amphora culture (3300-2700 BCE) existed over large parts of Central and Eastern Europe, but little is known about their interaction with neighboring Corded Ware groups and steppe societies. Here we present a detailed study of a Late Neolithic mass grave from southern Poland belonging to the Globular Amphora culture and containing the remains of 15 men, women, and children, all killed by blows to the head. We sequenced their genomes to between 1.1- and 3.9-fold coverage and performed kinship analyses that demonstrate that the individuals belonged to a large extended family. The bodies had been carefully laid out according to kin relationships by someone who evidently knew the deceased. From a population genetic viewpoint, the people from Koszyce are clearly distinct from neighboring Corded Ware groups because of their lack of steppe-related ancestry. Although the reason for the massacre is unknown, it is possible that it was connected with the expansion of Corded Ware groups, which may have resulted in competition for resources and violent conflict. Together with the archaeological evidence, these analyses provide an unprecedented level of insight into the kinship structure and social behavior of a Late Neolithic community.},
}

@article {pmid31056281,
year = {2019},
author = {Fages, A and Hanghøj, K and Khan, N and Gaunitz, C and Seguin-Orlando, A and Leonardi, M and McCrory Constantz, C and Gamba, C and Al-Rasheid, KAS and Albizuri, S and Alfarhan, AH and Allentoft, M and Alquraishi, S and Anthony, D and Baimukhanov, N and Barrett, JH and Bayarsaikhan, J and Benecke, N and Bernáldez-Sánchez, E and Berrocal-Rangel, L and Biglari, F and Boessenkool, S and Boldgiv, B and Brem, G and Brown, D and Burger, J and Crubézy, E and Daugnora, L and Davoudi, H and de Barros Damgaard, P and de Los Ángeles de Chorro Y de Villa-Ceballos, M and Deschler-Erb, S and Detry, C and Dill, N and do Mar Oom, M and Dohr, A and Ellingvåg, S and Erdenebaatar, D and Fathi, H and Felkel, S and Fernández-Rodríguez, C and García-Viñas, E and Germonpré, M and Granado, JD and Hallsson, JH and Hemmer, H and Hofreiter, M and Kasparov, A and Khasanov, M and Khazaeli, R and Kosintsev, P and Kristiansen, K and Kubatbek, T and Kuderna, L and Kuznetsov, P and Laleh, H and Leonard, JA and Lhuillier, J and Liesau von Lettow-Vorbeck, C and Logvin, A and Lõugas, L and Ludwig, A and Luis, C and Arruda, AM and Marques-Bonet, T and Matoso Silva, R and Merz, V and Mijiddorj, E and Miller, BK and Monchalov, O and Mohaseb, FA and Morales, A and Nieto-Espinet, A and Nistelberger, H and Onar, V and Pálsdóttir, AH and Pitulko, V and Pitskhelauri, K and Pruvost, M and Rajic Sikanjic, P and Rapan Papeša, A and Roslyakova, N and Sardari, A and Sauer, E and Schafberg, R and Scheu, A and Schibler, J and Schlumbaum, A and Serrand, N and Serres-Armero, A and Shapiro, B and Sheikhi Seno, S and Shevnina, I and Shidrang, S and Southon, J and Star, B and Sykes, N and Taheri, K and Taylor, W and Teegen, WR and Trbojević Vukičević, T and Trixl, S and Tumen, D and Undrakhbold, S and Usmanova, E and Vahdati, A and Valenzuela-Lamas, S and Viegas, C and Wallner, B and Weinstock, J and Zaibert, V and Clavel, B and Lepetz, S and Mashkour, M and Helgason, A and Stefánsson, K and Barrey, E and Willerslev, E and Outram, AK and Librado, P and Orlando, L},
title = {Tracking Five Millennia of Horse Management with Extensive Ancient Genome Time Series.},
journal = {Cell},
volume = {177},
number = {6},
pages = {1419-1435.e31},
doi = {10.1016/j.cell.2019.03.049},
pmid = {31056281},
issn = {1097-4172},
support = {U01 MH106874/MH/NIMH NIH HHS/United States ; },
abstract = {Horse domestication revolutionized warfare and accelerated travel, trade, and the geographic expansion of languages. Here, we present the largest DNA time series for a non-human organism to date, including genome-scale data from 149 ancient animals and 129 ancient genomes (≥1-fold coverage), 87 of which are new. This extensive dataset allows us to assess the modern legacy of past equestrian civilizations. We find that two extinct horse lineages existed during early domestication, one at the far western (Iberia) and the other at the far eastern range (Siberia) of Eurasia. None of these contributed significantly to modern diversity. We show that the influence of Persian-related horse lineages increased following the Islamic conquests in Europe and Asia. Multiple alleles associated with elite-racing, including at the MSTN "speed gene," only rose in popularity within the last millennium. Finally, the development of modern breeding impacted genetic diversity more dramatically than the previous millennia of human management.},
}

@article {pmid31040238,
year = {2019},
author = {Aouizerat, T and Gutman, I and Paz, Y and Maeir, AM and Gadot, Y and Gelman, D and Szitenberg, A and Drori, E and Pinkus, A and Schoemann, M and Kaplan, R and Ben-Gedalya, T and Coppenhagen-Glazer, S and Reich, E and Saragovi, A and Lipschits, O and Klutstein, M and Hazan, R},
title = {Isolation and Characterization of Live Yeast Cells from Ancient Vessels as a Tool in Bio-Archaeology.},
journal = {mBio},
volume = {10},
number = {2},
pages = {},
doi = {10.1128/mBio.00388-19},
pmid = {31040238},
issn = {2150-7511},
mesh = {Archaeology/*methods ; Fossils/*microbiology ; Genotype ; Geologic Sediments/*microbiology ; Microbiological Techniques/*methods ; Yeasts/*isolation & purification ; },
abstract = {Ancient fermented food has been studied based on recipes, residue analysis, and ancient-DNA techniques and reconstructed using modern domesticated yeast. Here, we present a novel approach based on our hypothesis that enriched yeast populations in fermented beverages could have become the dominant species in storage vessels and their descendants could be isolated and studied today. We developed a pipeline of yeast isolation from clay vessels and screened for yeast cells in beverage-related and non-beverage-related ancient vessels and sediments from several archaeological sites. We found that yeast cells could be successfully isolated specifically from clay containers of fermented beverages. The findings that genotypically the isolated yeasts are similar to those found in traditional African beverages and phenotypically they grow similar to modern beer-producing yeast strongly suggest that they are descendants of the original fermenting yeast. These results demonstrate that modern microorganisms can serve as a new tool in bio-archaeology research.IMPORTANCE So far, most of the study of ancient organisms has been based mainly on the analysis of ancient DNA. Here we show that it is possible to isolate and study microorganisms-yeast in this case-from ancient pottery vessels used for fermentation. We demonstrate that it is highly likely that these cells are descendants of the original yeast strains that participated in the fermentation process and were absorbed into the clay matrix of the pottery vessels. Moreover, we characterized the isolated yeast strains, their genomes, and the beer they produced. These results open new and exciting avenues in the study of domesticated microorganisms and contribute significantly to the fields of bio- and experimental archaeology that aim to reconstruct ancient artifacts and products.},
}

Archaeology/*methods
Fossils/*microbiology
Genotype
Geologic Sediments/*microbiology
Microbiological Techniques/*methods
Yeasts/*isolation & purification

@article {pmid31036834,
year = {2019},
author = {Stahlschmidt, MC and Collin, TC and Fernandes, DM and Bar-Oz, G and Belfer-Cohen, A and Gao, Z and Jakeli, N and Matskevich, Z and Meshveliani, T and Pritchard, JK and McDermott, F and Pinhasi, R},
title = {Ancient Mammalian and Plant DNA from Late Quaternary Stalagmite Layers at Solkota Cave, Georgia.},
journal = {Scientific reports},
volume = {9},
number = {1},
pages = {6628},
doi = {10.1038/s41598-019-43147-0},
pmid = {31036834},
issn = {2045-2322},
support = {GOIPD/775//Irish Research Council (An Chomhairle um Thaighde in Éirinn)/ ; },
abstract = {Metagenomic analysis is a highly promising technique in paleogenetic research that allows analysis of the complete genomic make-up of a sample. This technique has successfully been employed to archaeological sediments, but possible leaching of DNA through the sequence limits interpretation. We applied this technique to the analysis of ancient DNA (aDNA) from Late Quaternary stalagmites from two caves in Western Georgia, Melouri Cave and Solkota. Stalagmites form closed systems, limiting the effect of leaching, and can be securely dated with U-series. The analyses of the sequence data from the Melouri Cave stalagmite revealed potential contamination and low preservation of DNA. However, the two Solkota stalagmites preserved ancient DNA molecules of mammals (bear, roe deer, bats) and plants (chestnut, hazelnut, flax). The aDNA bearing layers from one of the two Solkota stalagmites were dated to between ~84 ka and ~56 ka BP by U-series. The second Solkota stalagmite contained excessive detrital clay obstructing U-series dating, but it also contained bear bones with a minimum age of ~50 BP uncalibrated years and ancient DNA molecules. The preservation of authentic ancient DNA molecules in Late Quaternary speleothems opens up a new paleogenetic archive for archaeological, paleontological and paleoenvironmental research.},
}

@article {pmid31036632,
year = {2019},
author = {Shaw, B and Burrell, CL and Green, D and Navarro-Martinez, A and Scott, D and Daroszewska, A and van 't Hof, R and Smith, L and Hargrave, F and Mistry, S and Bottrill, A and Kessler, BM and Fischer, R and Singh, A and Dalmay, T and Fraser, WD and Henneberger, K and King, T and Gonzalez, S and Layfield, R},
title = {Molecular insights into an ancient form of Paget's disease of bone.},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {116},
number = {21},
pages = {10463-10472},
doi = {10.1073/pnas.1820556116},
pmid = {31036632},
issn = {1091-6490},
support = {//Wellcome Trust/United Kingdom ; },
abstract = {Paget's disease of bone (PDB) is a chronic skeletal disorder that can affect one or several bones in individuals older than 55 y of age. PDB-like changes have been reported in archaeological remains as old as Roman, although accurate diagnosis and natural history of the disease is lacking. Six skeletons from a collection of 130 excavated at Norton Priory in the North West of England, which dates to medieval times, show atypical and extensive pathological changes resembling contemporary PDB affecting as many as 75% of individual skeletons. Disease prevalence in the remaining collection is high, at least 16% of adults, with age at death estimations as low as 35 y. Despite these atypical features, paleoproteomic analysis identified sequestosome 1 (SQSTM1) or p62, a protein central to the pathological milieu of PDB, as one of the few noncollagenous human sequences preserved in skeletal samples. Targeted proteomic analysis detected >60% of the ancient p62 primary sequence, with Western blotting indicating p62 abnormalities, including in dentition. Direct sequencing of ancient DNA excluded contemporary PDB-associated SQSTM1 mutations. Our observations indicate that the ancient p62 protein is likely modified within its C-terminal ubiquitin-associated domain. Ancient miRNAs were remarkably preserved in an osteosarcoma from a skeleton with extensive disease, with miR-16 expression consistent with that reported in contemporary PDB-associated bone tumors. Our work displays the use of proteomics to inform diagnosis of ancient diseases such as atypical PDB, which has unusual features presumably potentiated by yet-unidentified environmental or genetic factors.},
}

@article {pmid31013797,
year = {2019},
author = {Santiago-Rodriguez, TM and Fornaciari, A and Fornaciari, G and Luciani, S and Marota, I and Vercellotti, G and Toranzos, GA and Giuffra, V and Cano, RJ},
title = {Commensal and Pathogenic Members of the Dental Calculus Microbiome of Badia Pozzeveri Individuals from the 11th to 19th Centuries.},
journal = {Genes},
volume = {10},
number = {4},
pages = {},
doi = {10.3390/genes10040299},
pmid = {31013797},
issn = {2073-4425},
abstract = {The concept of the human oral microbiome was applied to understand health and disease, lifestyles, and dietary habits throughout part of human history. In the present study, we augment the understanding of ancient oral microbiomes by characterizing human dental calculus samples recovered from the ancient Abbey of Badia Pozzeveri (central Italy), with differences in socioeconomic status, time period, burial type, and sex. Samples dating from the Middle Ages (11th century) to the Industrial Revolution era (19th century) were characterized using high-throughput sequencing of the 16S ribosomal RNA (rRNA) gene V4 region. Consistent with previous studies, individuals from Badia Pozzeveri possessed commensal oral bacteria that resembled modern oral microbiomes. These results suggest that members of the oral microbiome are ubiquitous despite differences in geographical regions, time period, sex, and socioeconomic status. The presence of fecal bacteria could be in agreement with poor hygiene practices, consistent with the time period. Respiratory tract, nosocomial, and other rare pathogens detected in the dental calculus samples are intriguing and could suggest subject-specific comorbidities that could be reflected in the oral microbiome.},
}

@article {pmid31006515,
year = {2019},
author = {Haber, M and Doumet-Serhal, C and Scheib, CL and Xue, Y and Mikulski, R and Martiniano, R and Fischer-Genz, B and Schutkowski, H and Kivisild, T and Tyler-Smith, C},
title = {A Transient Pulse of Genetic Admixture from the Crusaders in the Near East Identified from Ancient Genome Sequences.},
journal = {American journal of human genetics},
volume = {104},
number = {5},
pages = {977-984},
doi = {10.1016/j.ajhg.2019.03.015},
pmid = {31006515},
issn = {1537-6605},
abstract = {During the medieval period, hundreds of thousands of Europeans migrated to the Near East to take part in the Crusades, and many of them settled in the newly established Christian states along the Eastern Mediterranean coast. Here, we present a genetic snapshot of these events and their aftermath by sequencing the whole genomes of 13 individuals who lived in what is today known as Lebanon between the 3rd and 13th centuries CE. These include nine individuals from the "Crusaders' pit" in Sidon, a mass burial in South Lebanon identified from the archaeology as the grave of Crusaders killed during a battle in the 13th century CE. We show that all of the Crusaders' pit individuals were males; some were Western Europeans from diverse origins, some were locals (genetically indistinguishable from present-day Lebanese), and two individuals were a mixture of European and Near Eastern ancestries, providing direct evidence that the Crusaders admixed with the local population. However, these mixtures appear to have had limited genetic consequences since signals of admixture with Europeans are not significant in any Lebanese group today-in particular, Lebanese Christians are today genetically similar to local people who lived during the Roman period which preceded the Crusades by more than four centuries.},
}

@article {pmid31004175,
year = {2019},
author = {Membrebe, JV and Suchard, MA and Rambaut, A and Baele, G and Lemey, P},
title = {Bayesian Inference of Evolutionary Histories under Time-Dependent Substitution Rates.},
journal = {Molecular biology and evolution},
volume = {36},
number = {8},
pages = {1793-1803},
doi = {10.1093/molbev/msz094},
pmid = {31004175},
issn = {1537-1719},
abstract = {Many factors complicate the estimation of time scales for phylogenetic histories, requiring increasingly complex evolutionary models and inference procedures. The widespread application of molecular clock dating has led to the insight that evolutionary rate estimates may vary with the time frame of measurement. This is particularly well established for rapidly evolving viruses that can accumulate sequence divergence over years or even months. However, this rapid evolution stands at odds with a relatively high degree of conservation of viruses or endogenous virus elements over much longer time scales. Building on recent insights into time-dependent evolutionary rates, we develop a formal and flexible Bayesian statistical inference approach that accommodates rate variation through time. We evaluate the novel molecular clock model on a foamy virus cospeciation history and a lentivirus evolutionary history and compare the performance to other molecular clock models. For both virus examples, we estimate a similarly strong time-dependent effect that implies rates varying over four orders of magnitude. The application of an analogous codon substitution model does not implicate long-term purifying selection as the cause of this effect. However, selection does appear to affect divergence time estimates for the less deep evolutionary history of the Ebolavirus genus. Finally, we explore the application of our approach on woolly mammoth ancient DNA data, which shows a much weaker, but still important, time-dependent rate effect that has a noticeable impact on node age estimates. Future developments aimed at incorporating more complex evolutionary processes will further add to the broad applicability of our approach.},
}

@article {pmid31004132,
year = {2019},
author = {Hanghøj, K and Renaud, G and Albrechtsen, A and Orlando, L},
title = {DamMet: ancient methylome mapping accounting for errors, true variants, and post-mortem DNA damage.},
journal = {GigaScience},
volume = {8},
number = {4},
pages = {},
doi = {10.1093/gigascience/giz025},
pmid = {31004132},
issn = {2047-217X},
abstract = {BACKGROUND: Recent computational advances in ancient DNA research have opened access to the detection of ancient DNA methylation footprints at the genome-wide scale. The most commonly used approach infers the methylation state of a given genomic region on the basis of the amount of nucleotide mis-incorporations observed at CpG dinucleotide sites. However, this approach overlooks a number of confounding factors, including the presence of sequencing errors and true variants. The scale and distribution of the inferred methylation measurements are also variable across samples, precluding direct comparisons.

FINDINGS: Here, we present DamMet, an open-source software program retrieving maximum likelihood estimates of regional CpG methylation levels from ancient DNA sequencing data. It builds on a novel statistical model of post-mortem DNA damage for dinucleotides, accounting for sequencing errors, genotypes, and differential post-mortem cytosine deamination rates at both methylated and unmethylated sites. To validate DamMet, we extended gargammel, a sequence simulator for ancient DNA data, by introducing methylation-dependent features of post-mortem DNA decay. This new simulator provides direct validation of DamMet predictions. Additionally, the methylation levels inferred by DamMet were found to be correlated to those inferred by epiPALEOMIX and both on par and directly comparable to those measured from whole-genome bisulphite sequencing experiments of fresh tissues.

CONCLUSIONS: DamMet provides genuine estimates for local DNA methylation levels in ancient individual genomes. The returned estimates are directly cross-sample comparable, and the software is available as an open-source C++ program hosted at https://gitlab.com/KHanghoj/DamMet along with a manual and tutorial.},
}

@article {pmid30994442,
year = {2019},
author = {Meffray, A and Perrin, M and Richier, A and Schmitt, A and Ardagna, Y and Biagini, P},
title = {Molecular detection of Treponema pallidum subspecies pallidum in 150-year-old foetal remains, southeastern France.},
journal = {Journal of medical microbiology},
volume = {68},
number = {5},
pages = {761-769},
doi = {10.1099/jmm.0.000978},
pmid = {30994442},
issn = {1473-5644},
mesh = {Archaeology ; Cemeteries ; DNA, Ancient/*isolation & purification ; Fetus/microbiology ; France ; History, 19th Century ; Humans ; Infant ; Mycobacterium tuberculosis/genetics ; Plasmodium falciparum/genetics ; Polymerase Chain Reaction ; Sequence Analysis, DNA ; Syphilis, Congenital/history/*microbiology ; Treponema pallidum/classification/*isolation & purification ; },
abstract = {PURPOSE: Syphilis, caused by Treponema pallidum subspecies pallidum , is considered as an old disease affecting humans; traces of such infections, including congenital syphilis, are potentially identifiable in archaeological samples. The aim of this research was to perform macroscopic and molecular investigations of T. pallidum on six infant remains, buried between 1837 and 1867, from the cemetery of 'Les Crottes' in Marseille city (southeastern France).

METHODOLOGY: Pathological analysis of bones from individuals, aged from the twenty-ninth week of amenorrhea to 4-9 months, was performed. Samples served also as a source of ancient DNA (aDNA) for PCR-based molecular investigations targeting T. pallidum DNA; all samples were also tested for Mycobacterium tuberculosis and Plasmodium falciparum DNA. Sequences characterized were cloned and sequenced, and compared to those available in databases.Results/Key findings. All samples tested displayed widespread osteoporotic lesions across the skeleton possibly related to some metabolic or infectious disorders. Subsequent molecular analysis revealed that one individual, SP332 (unborn, 29 amenorrhea weeks, inhumation date 1864-1866), exhibited positive signals for the five T. pallidum amplification systems tested; sequence analysis provided strong evidence for the effective detection of T. pallidum subspecies pallidum DNA.

CONCLUSIONS: Individual SP332 is the first PCR-confirmed palaeopathological case of syphilis identified in France, and the youngest specimen ever to be diagnosed with certainty for congenital syphilis. Future research aimed at better characterizing this 150-year-old treponeme genome and exploring new archaelogical cases of syphilis in the very young should contribute to a better comprehension of the disease's history.},
}

Archaeology
Cemeteries
DNA, Ancient/*isolation & purification
Fetus/microbiology
France
History, 19th Century
Humans
Infant
Mycobacterium tuberculosis/genetics
Plasmodium falciparum/genetics
Polymerase Chain Reaction
Sequence Analysis, DNA
Syphilis, Congenital/history/*microbiology
Treponema pallidum/classification/*isolation & purification

@article {pmid30992585,
year = {2019},
author = {Phillips, N},
title = {Indigenous groups look to ancient DNA to bring their ancestors home.},
journal = {Nature},
volume = {568},
number = {7752},
pages = {294-297},
doi = {10.1038/d41586-019-01167-w},
pmid = {30992585},
issn = {1476-4687},
}

@article {pmid30988490,
year = {2019},
author = {Brace, S and Diekmann, Y and Booth, TJ and van Dorp, L and Faltyskova, Z and Rohland, N and Mallick, S and Olalde, I and Ferry, M and Michel, M and Oppenheimer, J and Broomandkhoshbacht, N and Stewardson, K and Martiniano, R and Walsh, S and Kayser, M and Charlton, S and Hellenthal, G and Armit, I and Schulting, R and Craig, OE and Sheridan, A and Parker Pearson, M and Stringer, C and Reich, D and Thomas, MG and Barnes, I},
title = {Ancient genomes indicate population replacement in Early Neolithic Britain.},
journal = {Nature ecology & evolution},
volume = {3},
number = {5},
pages = {765-771},
doi = {10.1038/s41559-019-0871-9},
pmid = {30988490},
issn = {2397-334X},
support = {//Wellcome Trust/United Kingdom ; 100713//Wellcome Trust/United Kingdom ; R01 GM100233/GM/NIGMS NIH HHS/United States ; },
abstract = {The roles of migration, admixture and acculturation in the European transition to farming have been debated for over 100 years. Genome-wide ancient DNA studies indicate predominantly Aegean ancestry for continental Neolithic farmers, but also variable admixture with local Mesolithic hunter-gatherers. Neolithic cultures first appear in Britain circa 4000 BC, a millennium after they appeared in adjacent areas of continental Europe. The pattern and process of this delayed British Neolithic transition remain unclear. We assembled genome-wide data from 6 Mesolithic and 67 Neolithic individuals found in Britain, dating 8500-2500 BC. Our analyses reveal persistent genetic affinities between Mesolithic British and Western European hunter-gatherers. We find overwhelming support for agriculture being introduced to Britain by incoming continental farmers, with small, geographically structured levels of hunter-gatherer ancestry. Unlike other European Neolithic populations, we detect no resurgence of hunter-gatherer ancestry at any time during the Neolithic in Britain. Genetic affinities with Iberian Neolithic individuals indicate that British Neolithic people were mostly descended from Aegean farmers who followed the Mediterranean route of dispersal. We also infer considerable variation in pigmentation levels in Europe by circa 6000 BC.},
}

@article {pmid30980085,
year = {2019},
author = {Kutanan, W and Kampuansai, J and Srikummool, M and Brunelli, A and Ghirotto, S and Arias, L and Macholdt, E and Hübner, A and Schröder, R and Stoneking, M},
title = {Contrasting Paternal and Maternal Genetic Histories of Thai and Lao Populations.},
journal = {Molecular biology and evolution},
volume = {36},
number = {7},
pages = {1490-1506},
doi = {10.1093/molbev/msz083},
pmid = {30980085},
issn = {1537-1719},
abstract = {The human demographic history of Mainland Southeast Asia (MSEA) has not been well studied; in particular, there have been very few sequence-based studies of variation in the male-specific portions of the Y chromosome (MSY). Here, we report new MSY sequences of ∼2.3 mB from 914 males and combine these with previous data for a total of 928 MSY sequences belonging to 59 populations from Thailand and Laos who speak languages belonging to three major Mainland Southeast Asia families: Austroasiatic, Tai-Kadai, and Sino-Tibetan. Among the 92 MSY haplogroups, two main MSY lineages (O1b1a1a* [O-M95*] and O2a* [O-M324*]) contribute substantially to the paternal genetic makeup of Thailand and Laos. We also analyze complete mitochondrial DNA genome sequences published previously from the same groups and find contrasting pattern of male and female genetic variation and demographic expansions, especially for the hill tribes, Mon, and some major Thai groups. In particular, we detect an effect of postmarital residence pattern on genetic diversity in patrilocal versus matrilocal groups. Additionally, both male and female demographic expansions were observed during the early Mesolithic (∼10 ka), with two later major male-specific expansions during the Neolithic period (∼4-5 ka) and the Bronze/Iron Age (∼2.0-2.5 ka). These two later expansions are characteristic of the modern Austroasiatic and Tai-Kadai groups, respectively, consistent with recent ancient DNA studies. We simulate MSY data based on three demographic models (continuous migration, demic diffusion, and cultural diffusion) of major Thai groups and find different results from mitochondrial DNA simulations, supporting contrasting male and female genetic histories.},
}

@article {pmid30977781,
year = {2019},
author = {Oliva, A and Pulicani, S and Lefort, V and Bréhélin, L and Gascuel, O and Guindon, S},
title = {Accounting for ambiguity in ancestral sequence reconstruction.},
journal = {Bioinformatics (Oxford, England)},
volume = {},
number = {},
pages = {},
doi = {10.1093/bioinformatics/btz249},
pmid = {30977781},
issn = {1367-4811},
abstract = {MOTIVATION: The reconstruction of ancestral genetic sequences from the analysis of contemporaneous data is a powerful tool to improve our understanding of molecular evolution. Various statistical criteria defined in a phylogenetic framework can be used to infer nucleotide, amino-acid or codon states at internal nodes of the tree, for every position along the sequence. These criteria generally select the state that maximizes (or minimizes) a given criterion. Although it is perfectly sensible from a statistical perspective, that strategy fails to convey useful information about the level of uncertainty associated to the inference.

RESULTS: The present study introduces a new criterion for ancestral sequence reconstruction, the minimum posterior expected error (MPEE), that selects a single state whenever the signal conveyed by the data is strong, and a combination of multiple states otherwise. We also assess the performance of a criterion based on the Brier scoring scheme which, like MPEE, does not rely on any tuning parameters. The precision and accuracy of several other criteria that involve arbitrarily set tuning parameters are also evaluated. Large scale simulations demonstrate the benefits of using the MPEE and Brier-based criteria with a substantial increase in the accuracy of the inference of past sequences compared to the standard approach and realistic compromises on the precision of the solutions returned.

AVAILABILITY: The software package PhyML (https://github.com/stephaneguindon/phyml) provides an implementation of the Maximum A Posteriori (MAP) and MPEE criteria for reconstructing ancestral nucleotide and amino-acid sequences.},
}

@article {pmid30972043,
year = {2019},
author = {Mills, JG and Brookes, JD and Gellie, NJC and Liddicoat, C and Lowe, AJ and Sydnor, HR and Thomas, T and Weinstein, P and Weyrich, LS and Breed, MF},
title = {Relating Urban Biodiversity to Human Health With the 'Holobiont' Concept.},
journal = {Frontiers in microbiology},
volume = {10},
number = {},
pages = {550},
doi = {10.3389/fmicb.2019.00550},
pmid = {30972043},
issn = {1664-302X},
abstract = {A relatively unaccounted ecosystem service from biodiversity is the benefit to human health via symbiotic microbiota from our environment. This benefit occurs because humans evolved alongside microbes and have been constantly exposed to diverse microbiota. Plants and animals, including humans, are organised as a host with symbiotic microbiota, whose collective genome and life history form a single holobiont. As such, there are interdependencies between biodiversity, holobionts, and public health which lead us to argue that human health outcomes could be improved by increasing contact with biodiversity in an urban context. We propose that humans, like all holobionts, likely require a diverse microbial habitat to appropriate resources for living healthy, long lives. We discuss how industrial urbanisation likely disrupts the symbiosis between microbiota and their hosts, leading to negative health outcomes. The industrialised urban habitat is low in macro and microbial biodiversity and discourages contact with beneficial environmental microbiota. These habitat factors, alongside diet, antibiotics, and others, are associated with the epidemic of non-communicable diseases in these societies. We suggest that restoration of urban microbial biodiversity and micro-ecological processes through microbiome rewilding can benefit holobiont health and aid in treating the urban non-communicable disease epidemic. Further, we identify research gaps and some solutions to economic and strategic hurdles in applying microbiome rewilding into daily urban life.},
}

@article {pmid30964548,
year = {2019},
author = {Klunk, J and Duggan, AT and Redfern, R and Gamble, J and Boldsen, JL and Golding, GB and Walter, BS and Eaton, K and Stangroom, J and Rouillard, JM and Devault, A and DeWitte, SN and Poinar, HN},
title = {Genetic resiliency and the Black Death: No apparent loss of mitogenomic diversity due to the Black Death in medieval London and Denmark.},
journal = {American journal of physical anthropology},
volume = {169},
number = {2},
pages = {240-252},
doi = {10.1002/ajpa.23820},
pmid = {30964548},
issn = {1096-8644},
support = {APP1065106//National Health and Medical Research Council (NHMRC)/ ; RGPIN-2015-04477//Natural Sciences and Engineering Research Council of Canada (NSERC)/ ; 430-2017-01193//Social Sciences and Humanities Research Council of Canada (SSHRC)/ ; 435-2018-0465//Social Sciences and Humanities Research Council of Canada (SSHRC)/ ; //Arbor Biosciences/ ; //McMaster University/ ; //Canada Research Chair Program/ ; //University of Toronto/ ; },
abstract = {OBJECTIVES: In the 14th century AD, medieval Europe was severely affected by the Great European Famine as well as repeated bouts of disease, including the Black Death, causing major demographic shifts. This high volatility led to increased mobility and migration due to new labor and economic opportunities, as evidenced by documentary and stable isotope data. This study uses ancient DNA (aDNA) isolated from skeletal remains to examine whether evidence for large-scale population movement can be gleaned from the complete mitochondrial genomes of 264 medieval individuals from England (London) and Denmark.

MATERIALS AND METHODS: Using a novel library-conserving approach to targeted capture, we recovered 264 full mitochondrial genomes from the petrous portion of the temporal bones and teeth and compared genetic diversity across the medieval period within and between English (London) and Danish populations and with contemporary populations through population pairwise ΦST analysis.

RESULTS: We find no evidence of significant differences in genetic diversity spatially or temporally in our dataset, yet there is a high degree of haplotype diversity in our medieval samples with little exact sequence sharing.

DISCUSSION: The mitochondrial genomes of both medieval Londoners and medieval Danes suggest high mitochondrial diversity before, during and after the Black Death. While our mitochondrial genomic data lack geographically correlated signals, these data could be the result of high, continual female migration before and after the Black Death or may simply indicate a large female effective population size unaffected by the upheaval of the medieval period. Either scenario suggests a genetic resiliency in areas of northwestern medieval Europe.},
}

@article {pmid30963935,
year = {2019},
author = {Nakagome, S and Hudson, RR and Di Rienzo, A},
title = {Inferring the model and onset of natural selection under varying population size from the site frequency spectrum and haplotype structure.},
journal = {Proceedings. Biological sciences},
volume = {286},
number = {1896},
pages = {20182541},
doi = {10.1098/rspb.2018.2541},
pmid = {30963935},
issn = {1471-2954},
abstract = {A fundamental question about adaptation in a population is the time of onset of the selective pressure acting on beneficial alleles. Inferring this time, in turn, depends on the selection model. We develop a framework of approximate Bayesian computation (ABC) that enables the use of the full site frequency spectrum and haplotype structure to test the goodness-of-fit of selection models and estimate the timing of selection under varying population size scenarios. We show that our method has sufficient power to distinguish natural selection from neutrality even if relatively old selection increased the frequency of a pre-existing allele from 20% to 50% or from 40% to 80%. Our ABC can accurately estimate the time of onset of selection on a new mutation. However, estimates are prone to bias under the standing variation model, possibly due to the uncertainty in the allele frequency at the onset of selection. We further extend our approach to take advantage of ancient DNA data that provides information on the allele frequency path of the beneficial allele. Applying our ABC, including both modern and ancient human DNA data, to four pigmentation alleles in Europeans, we detected selection on standing variants that occurred after the dispersal from Africa even though models of selection on a new mutation were initially supported for two of these alleles without the ancient data.},
}

@article {pmid30957607,
year = {2019},
author = {Siripan, S and Wonnapinij, P and Auetrakulvit, P and Wangthongchaicharoen, N and Surat, W},
title = {Origin of prehistoric cattle excavated from four archaeological sites in central and northeastern Thailand.},
journal = {Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis},
volume = {30},
number = {4},
pages = {609-617},
doi = {10.1080/24701394.2019.1597072},
pmid = {30957607},
issn = {2470-1408},
abstract = {Cattle have been domesticated in Southeast Asia, including Thailand, for thousands of years, but the history of cattle domestication in the region remains unclear. To date, only genetic studies of modern Thai cattle DNA have been reported. To gain some insight into cattle domestication in the country, a total of 56 cattle remains excavated from four archaeological sites (dated to between 3550 and 1700 years before present (YBP)) in northeastern and central Thailand were analysed in this study. Of 56, the 157-bp D-loop fragment was successfully generated from 26 samples, all of which belonged to Bos taurus in haplogroup T/T3. One haplotype contained 19 members from all four archaeological sites and clustered with the ancient B. taurus from Iran, Turkey and China. Other haplotypes have not shared haplotype with B. taurus from other countries but they showed close relationship to those from China. This represents the first genetic evidence that B.taurus was domesticated in Thailand between 3550 and 1700 YBP. In addition, the close relationship among ancient Thai, Iranian and Chinese taurines suggests that cattle from the Near East were introduced into North China, and were subsequently brought into Thailand thousands of years ago.},
}

@article {pmid30954285,
year = {2019},
author = {Brunson, K and Reich, D},
title = {The Promise of Paleogenomics Beyond Our Own Species.},
journal = {Trends in genetics : TIG},
volume = {35},
number = {5},
pages = {319-329},
doi = {10.1016/j.tig.2019.02.006},
pmid = {30954285},
issn = {0168-9525},
abstract = {Paleogenomics, also known as genome-wide ancient DNA analysis, is transforming our understanding of the human past, but has been much less intensively used to understand the history of other species. However, paleogenomic studies of non-human animals and plants have the potential to address an equally rich range of evolutionary, paleoecological, paleoenvironmental, and archaeological research questions. Three recent case studies of cave bears, horses, and maize provide examples of the ways that paleogenomics can be used to examine potential causes of extinctions and dynamic processes of domestication. Much more research in these areas is needed, and we conclude by highlighting key future directions.},
}

@article {pmid30953039,
year = {2019},
author = {Spyrou, MA and Bos, KI and Herbig, A and Krause, J},
title = {Ancient pathogen genomics as an emerging tool for infectious disease research.},
journal = {Nature reviews. Genetics},
volume = {20},
number = {6},
pages = {323-340},
doi = {10.1038/s41576-019-0119-1},
pmid = {30953039},
issn = {1471-0064},
mesh = {Archaea/genetics/isolation & purification ; Bacteria/genetics/isolation & purification ; Biological Evolution ; Communicable Diseases/*history/microbiology/parasitology/virology ; DNA, Ancient/*analysis/isolation & purification ; Fossils ; *Genome ; Genomics/*methods ; Global Health/history ; High-Throughput Nucleotide Sequencing/*methods ; History, 19th Century ; History, 21st Century ; History, Ancient ; History, Medieval ; Humans ; Public Health Surveillance/methods ; Viruses/genetics/isolation & purification ; },
abstract = {Over the past decade, a genomics revolution, made possible through the development of high-throughput sequencing, has triggered considerable progress in the study of ancient DNA, enabling complete genomes of past organisms to be reconstructed. A newly established branch of this field, ancient pathogen genomics, affords an in-depth view of microbial evolution by providing a molecular fossil record for a number of human-associated pathogens. Recent accomplishments include the confident identification of causative agents from past pandemics, the discovery of microbial lineages that are now extinct, the extrapolation of past emergence events on a chronological scale and the characterization of long-term evolutionary history of microorganisms that remain relevant to public health today. In this Review, we discuss methodological advancements, persistent challenges and novel revelations gained through the study of ancient pathogen genomes.},
}

Archaea/genetics/isolation & purification
Bacteria/genetics/isolation & purification
Biological Evolution
Communicable Diseases/*history/microbiology/parasitology/virology
DNA, Ancient/*analysis/isolation & purification
Fossils
*Genome
Genomics/*methods
Global Health/history
High-Throughput Nucleotide Sequencing/*methods
History, 19th Century
History, 21st Century
History, Ancient
History, Medieval
Humans
Public Health Surveillance/methods
Viruses/genetics/isolation & purification

@article {pmid30952160,
year = {2019},
author = {Yelmen, B and Mondal, M and Marnetto, D and Pathak, AK and Montinaro, F and Gallego Romero, I and Kivisild, T and Metspalu, M and Pagani, L},
title = {Ancestry-Specific Analyses Reveal Differential Demographic Histories and Opposite Selective Pressures in Modern South Asian Populations.},
journal = {Molecular biology and evolution},
volume = {36},
number = {8},
pages = {1628-1642},
doi = {10.1093/molbev/msz037},
pmid = {30952160},
issn = {1537-1719},
abstract = {Genetic variation in contemporary South Asian populations follows a northwest to southeast decreasing cline of shared West Eurasian ancestry. A growing body of ancient DNA evidence is being used to build increasingly more realistic models of demographic changes in the last few thousand years. Through high-quality modern genomes, these models can be tested for gene and genome level deviations. Using local ancestry deconvolution and masking, we reconstructed population-specific surrogates of the two main ancestral components for more than 500 samples from 25 South Asian populations and showed our approach to be robust via coalescent simulations. Our f3 and f4 statistics-based estimates reveal that the reconstructed haplotypes are good proxies for the source populations that admixed in the area and point to complex interpopulation relationships within the West Eurasian component, compatible with multiple waves of arrival, as opposed to a simpler one wave scenario. Our approach also provides reliable local haplotypes for future downstream analyses. As one such example, the local ancestry deconvolution in South Asians reveals opposite selective pressures on two pigmentation genes (SLC45A2 and SLC24A5) that are common or fixed in West Eurasians, suggesting post-admixture purifying and positive selection signals, respectively.},
}

@article {pmid30944772,
year = {2019},
author = {Ciucani, MM and Palumbo, D and Galaverni, M and Serventi, P and Fabbri, E and Ravegnini, G and Angelini, S and Maini, E and Persico, D and Caniglia, R and Cilli, E},
title = {Old wild wolves: ancient DNA survey unveils population dynamics in Late Pleistocene and Holocene Italian remains.},
journal = {PeerJ},
volume = {7},
number = {},
pages = {e6424},
doi = {10.7717/peerj.6424},
pmid = {30944772},
issn = {2167-8359},
abstract = {Background: The contemporary Italian wolf (Canis lupus italicus) represents a case of morphological and genetic uniqueness. Today, Italian wolves are also the only documented population to fall exclusively within the mitochondrial haplogroup 2, which was the most diffused across Eurasian and North American wolves during the Late Pleistocene. However, the dynamics leading to such distinctiveness are still debated.

Methods: In order to shed light on the ancient genetic variability of this wolf population and on the origin of its current diversity, we collected 19 Late Pleistocene-Holocene samples from northern Italy, which we analyzed at a short portion of the hypervariable region 1 of the mitochondrial DNA, highly informative for wolf and dog phylogenetic analyses.

Results: Four out of the six detected haplotypes matched the ones found in ancient wolves from northern Europe and Beringia, or in modern European and Chinese wolves, and appeared closely related to the two haplotypes currently found in Italian wolves. The haplotype of two Late Pleistocene samples matched with primitive and contemporary dog sequences from the canine mitochondrial clade A. All these haplotypes belonged to haplogroup 2. The only exception was a Holocene sample dated 3,250 years ago, affiliated to haplogroup 1.

Discussion: In this study we describe the genetic variability of the most ancient wolf specimens from Italy analyzed so far, providing a preliminary overview of the genetic make-up of the population that inhabited this area from the last glacial maximum to the Middle Age period. Our results endorsed that the genetic diversity carried by the Pleistocene wolves here analyzed showed a strong continuity with other northern Eurasian wolf specimens from the same chronological period. Contrarily, the Holocene samples showed a greater similarity only with modern sequences from Europe and Asia, and the occurrence of an haplogroup 1 haplotype allowed to date back previous finding about its presence in this area. Moreover, the unexpected discovery of a 24,700-year-old sample carrying a haplotype that, from the fragment here obtained, falls within the canine clade A, could represent the oldest evidence in Europe of such dog-rich clade. All these findings suggest complex population dynamics that deserve to be further investigated based on mitochondrial or whole genome sequencing.},
}

@article {pmid30942856,
year = {2019},
author = {Harris, DN and Ruczinski, I and Yanek, LR and Becker, LC and Becker, DM and Guio, H and Cui, T and Chilton, FH and Mathias, RA and O'Connor, TD},
title = {Evolution of Hominin Polyunsaturated Fatty Acid Metabolism: From Africa to the New World.},
journal = {Genome biology and evolution},
volume = {11},
number = {5},
pages = {1417-1430},
doi = {10.1093/gbe/evz071},
pmid = {30942856},
issn = {1759-6653},
support = {R01 AT008621/AT/NCCIH NIH HHS/United States ; },
abstract = {The metabolic conversion of dietary omega-3 and omega-6 18 carbon (18C) to long chain (>20 carbon) polyunsaturated fatty acids (LC-PUFAs) is vital for human life. The rate-limiting steps of this process are catalyzed by fatty acid desaturase (FADS) 1 and 2. Therefore, understanding the evolutionary history of the FADS genes is essential to our understanding of hominin evolution. The FADS genes have two haplogroups, ancestral and derived, with the derived haplogroup being associated with more efficient LC-PUFA biosynthesis than the ancestral haplogroup. In addition, there is a complex global distribution of these haplogroups that is suggestive of Neanderthal introgression. We confirm that Native American ancestry is nearly fixed for the ancestral haplogroup, and replicate a positive selection signal in Native Americans. This positive selection potentially continued after the founding of the Americas, although simulations suggest that the timing is dependent on the allele frequency of the ancestral Beringian population. We also find that the Neanderthal FADS haplotype is more closely related to the derived haplogroup and the Denisovan clusters closer to the ancestral haplogroup. Furthermore, the derived haplogroup has a time to the most recent common ancestor of 688,474 years before present. These results support an ancient polymorphism, as opposed to Neanderthal introgression, forming in the FADS region during the Pleistocene with possibly differential selection pressures on both haplogroups. The near fixation of the ancestral haplogroup in Native American ancestry calls for future studies to explore the potential health risk of associated low LC-PUFA levels in these populations.},
}

@article {pmid30913508,
year = {2019},
author = {Traversari, M and Serrangeli, MC and Catalano, G and Petrella, E and Piciucchi, S and Feletti, F and Oxilia, G and Cristiani, E and Vazzana, A and Sorrentino, R and De Fanti, S and Luiselli, D and Calcagnile, L and Saragoni, L and Feeney, RNM and Gruppioni, G and Cilli, E and Benazzi, S},
title = {Multi-analytic study of a probable case of fibrous dysplasia (FD) from certosa monumental cemetery (Bologna, Italy).},
journal = {International journal of paleopathology},
volume = {25},
number = {},
pages = {1-8},
doi = {10.1016/j.ijpp.2019.03.003},
pmid = {30913508},
issn = {1879-9825},
abstract = {OBJECTIVE: To evaluate, via a multidisciplinary approach, a distinctive paleopathological condition believed to be fibrous dysplasia, found on a 19th/20th century skeleton from Certosa Monumental Cemetery, Bologna, Italy.

MATERIALS: A skeletonized cranium and mandible recovered from an ossuary in 2014.

METHODS: Pathological alterations were analysed by radiological examination, dental macrowear, histopathological and genetic analyses.

RESULT: The skeleton is believed to be an adult male. Differential diagnoses include Paget's disease, McCune-Albright syndrome, osteochondroma and osteosarcoma. The radiographic findings, along with the solitary nature of the lesions, are strong evidence for the diagnosis of fibrous dysplasia (FD). Genetic analysis further revealed a frequency of ˜1% of mutant alleles with the R201C substitution, one of the post-zygotic activating mutation frequently associated with FD.

CONCLUSIONS: The multi-analytical method employed suggests a diagnosis of monostotic form of FD. The diagnostic design incorporates multiple lines of evidence, including macroscopic, histopathological, and genetic analyses.

SIGNIFICANCE: Through the use of a multi-analytic approach, robust diagnoses can be offered. This case serves as one of the oldest examples of FD from an historical context. The genetic mutation detected, associated with FD, has not been previously reported in historical/ancient samples.},
}

@article {pmid30893326,
year = {2019},
author = {Di Maida, G and Mannino, MA and Krause-Kyora, B and Jensen, TZT and Talamo, S},
title = {Radiocarbon dating and isotope analysis on the purported Aurignacian skeletal remains from Fontana Nuova (Ragusa, Italy).},
journal = {PloS one},
volume = {14},
number = {3},
pages = {e0213173},
doi = {10.1371/journal.pone.0213173},
pmid = {30893326},
issn = {1932-6203},
abstract = {Proving voyaging at sea by Palaeolithic humans is a difficult archaeological task, even for short distances. In the Mediterranean, a commonly accepted sea crossing is that from the Italian Peninsula to Sicily by anatomically modern humans, purportedly of the Aurignacian culture. This claim, however, was only supported by the typological attribution to the Aurignacian of the lithic industries from the insular site of Fontana Nuova. AMS radiocarbon dating undertaken as part of our research shows that the faunal remains, previously considered Aurignacian, actually date to the Holocene. Absolute dating on dentinal collagen also attributes the human teeth from the site to the early Holocene, although we were unable to obtain ancient DNA to evaluate their ancestry. Ten radiocarbon dates on human and other taxa are comprised between 9910-9700 cal. BP and 8600-8480 cal. BP, indicating that Fontana Nuova was occupied by Mesolithic and not Aurignacian hunter-gatherers. Only a new study of the lithic assemblage could establish if the material from Fontana Nuova is a mixed collection that includes both late Upper Palaeolithic (Epigravettian) and Mesolithic artefacts, as can be suggested by taking into account both the results of our study and of the most recent reinterpretation of the lithics. Nevertheless, this research suggests that the notion that Aurignacian groups were present in Sicily should now be revised. Another outcome of our study is that we found that three specimens, attributed on grounds both of morphological and ZooMS identifications to Cervus elaphus, had δ13C values significantly higher than any available for such species in Europe.},
}

@article {pmid30890703,
year = {2019},
author = {Feldman, M and Fernández-Domínguez, E and Reynolds, L and Baird, D and Pearson, J and Hershkovitz, I and May, H and Goring-Morris, N and Benz, M and Gresky, J and Bianco, RA and Fairbairn, A and Mustafaoğlu, G and Stockhammer, PW and Posth, C and Haak, W and Jeong, C and Krause, J},
title = {Late Pleistocene human genome suggests a local origin for the first farmers of central Anatolia.},
journal = {Nature communications},
volume = {10},
number = {1},
pages = {1218},
doi = {10.1038/s41467-019-09209-7},
pmid = {30890703},
issn = {2041-1723},
mesh = {Adult ; Agriculture/*history ; Archaeology ; Bone and Bones ; DNA, Ancient/*analysis/isolation & purification ; Europe ; Farmers/*history ; Female ; Genome, Human/*genetics ; High-Throughput Nucleotide Sequencing ; History, Ancient ; Human Migration/*history ; Humans ; Iran ; Male ; Radiometric Dating ; },
abstract = {Anatolia was home to some of the earliest farming communities. It has been long debated whether a migration of farming groups introduced agriculture to central Anatolia. Here, we report the first genome-wide data from a 15,000-year-old Anatolian hunter-gatherer and from seven Anatolian and Levantine early farmers. We find high genetic continuity (~80-90%) between the hunter-gatherers and early farmers of Anatolia and detect two distinct incoming ancestries: an early Iranian/Caucasus related one and a later one linked to the ancient Levant. Finally, we observe a genetic link between southern Europe and the Near East predating 15,000 years ago. Our results suggest a limited role of human migration in the emergence of agriculture in central Anatolia.},
}

Adult
Agriculture/*history
Archaeology
Bone and Bones
DNA, Ancient/*analysis/isolation & purification
Europe
Farmers/*history
Female
Genome, Human/*genetics
High-Throughput Nucleotide Sequencing
History, Ancient
Human Migration/*history
Humans
Iran
Male
Radiometric Dating

@article {pmid30887686,
year = {2019},
author = {Weyrich, LS and Farrer, AG and Eisenhofer, R and Arriola, LA and Young, J and Selway, CA and Handsley-Davis, M and Adler, CJ and Breen, J and Cooper, A},
title = {Laboratory contamination over time during low-biomass sample analysis.},
journal = {Molecular ecology resources},
volume = {19},
number = {4},
pages = {982-996},
doi = {10.1111/1755-0998.13011},
pmid = {30887686},
issn = {1755-0998},
support = {DECRA 150101574//Australian Research Council/ ; },
abstract = {Bacteria are not only ubiquitous on earth but can also be incredibly diverse within clean laboratories and reagents. The presence of both living and dead bacteria in laboratory environments and reagents is especially problematic when examining samples with low endogenous content (e.g., skin swabs, tissue biopsies, ice, water, degraded forensic samples or ancient material), where contaminants can outnumber endogenous microorganisms within samples. The contribution of contaminants within high-throughput studies remains poorly understood because of the relatively low number of contaminant surveys. Here, we examined 144 negative control samples (extraction blank and no-template amplification controls) collected in both typical molecular laboratories and an ultraclean ancient DNA laboratory over 5 years to characterize long-term contaminant diversity. We additionally compared the contaminant content within a home-made silica-based extraction method, commonly used to analyse low endogenous content samples, with a widely used commercial DNA extraction kit. The contaminant taxonomic profile of the ultraclean ancient DNA laboratory was unique compared to modern molecular biology laboratories, and changed over time according to researcher, month and season. The commercial kit also contained higher microbial diversity and several human-associated taxa in comparison to the home-made silica extraction protocol. We recommend a minimum of two strategies to reduce the impacts of laboratory contaminants within low-biomass metagenomic studies: (a) extraction blank controls should be included and sequenced with every batch of extractions and (b) the contributions of laboratory contamination should be assessed and reported in each high-throughput metagenomic study.},
}

@article {pmid30886779,
year = {2019},
author = {Eisenhofer, R and Weyrich, LS},
title = {Assessing alignment-based taxonomic classification of ancient microbial DNA.},
journal = {PeerJ},
volume = {7},
number = {},
pages = {e6594},
doi = {10.7717/peerj.6594},
pmid = {30886779},
issn = {2167-8359},
abstract = {The field of palaeomicrobiology-the study of ancient microorganisms-is rapidly growing due to recent methodological and technological advancements. It is now possible to obtain vast quantities of DNA data from ancient specimens in a high-throughput manner and use this information to investigate the dynamics and evolution of past microbial communities. However, we still know very little about how the characteristics of ancient DNA influence our ability to accurately assign microbial taxonomies (i.e. identify species) within ancient metagenomic samples. Here, we use both simulated and published metagenomic data sets to investigate how ancient DNA characteristics affect alignment-based taxonomic classification. We find that nucleotide-to-nucleotide, rather than nucleotide-to-protein, alignments are preferable when assigning taxonomies to short DNA fragment lengths routinely identified within ancient specimens (<60 bp). We determine that deamination (a form of ancient DNA damage) and random sequence substitutions corresponding to ∼100,000 years of genomic divergence minimally impact alignment-based classification. We also test four different reference databases and find that database choice can significantly bias the results of alignment-based taxonomic classification in ancient metagenomic studies. Finally, we perform a reanalysis of previously published ancient dental calculus data, increasing the number of microbial DNA sequences assigned taxonomically by an average of 64.2-fold and identifying microbial species previously unidentified in the original study. Overall, this study enhances our understanding of how ancient DNA characteristics influence alignment-based taxonomic classification of ancient microorganisms and provides recommendations for future palaeomicrobiological studies.},
}

@article {pmid30884759,
year = {2019},
author = {Abondio, P and Sazzini, M and Garagnani, P and Boattini, A and Monti, D and Franceschi, C and Luiselli, D and Giuliani, C},
title = {The Genetic Variability of APOE in Different Human Populations and Its Implications for Longevity.},
journal = {Genes},
volume = {10},
number = {3},
pages = {},
doi = {10.3390/genes10030222},
pmid = {30884759},
issn = {2073-4425},
abstract = {Human longevity is a complex phenotype resulting from the combinations of context-dependent gene-environment interactions that require analysis as a dynamic process in a cohesive ecological and evolutionary framework. Genome-wide association (GWAS) and whole-genome sequencing (WGS) studies on centenarians pointed toward the inclusion of the apolipoprotein E (APOE) polymorphisms ε2 and ε4, as implicated in the attainment of extreme longevity, which refers to their effect in age-related Alzheimer's disease (AD) and cardiovascular disease (CVD). In this case, the available literature on APOE and its involvement in longevity is described according to an anthropological and population genetics perspective. This aims to highlight the evolutionary history of this gene, how its participation in several biological pathways relates to human longevity, and which evolutionary dynamics may have shaped the distribution of APOE haplotypes across the globe. Its potential adaptive role will be described along with implications for the study of longevity in different human groups. This review also presents an updated overview of the worldwide distribution of APOE alleles based on modern day data from public databases and ancient DNA samples retrieved from literature in the attempt to understand the spatial and temporal frame in which present-day patterns of APOE variation evolved.},
}

@article {pmid30880015,
year = {2019},
author = {Villalba-Mouco, V and van de Loosdrecht, MS and Posth, C and Mora, R and Martínez-Moreno, J and Rojo-Guerra, M and Salazar-García, DC and Royo-Guillén, JI and Kunst, M and Rougier, H and Crevecoeur, I and Arcusa-Magallón, H and Tejedor-Rodríguez, C and García-Martínez de Lagrán, I and Garrido-Pena, R and Alt, KW and Jeong, C and Schiffels, S and Utrilla, P and Krause, J and Haak, W},
title = {Survival of Late Pleistocene Hunter-Gatherer Ancestry in the Iberian Peninsula.},
journal = {Current biology : CB},
volume = {29},
number = {7},
pages = {1169-1177.e7},
doi = {10.1016/j.cub.2019.02.006},
pmid = {30880015},
issn = {1879-0445},
abstract = {The Iberian Peninsula in southwestern Europe represents an important test case for the study of human population movements during prehistoric periods. During the Last Glacial Maximum (LGM), the peninsula formed a periglacial refugium [1] for hunter-gatherers (HGs) and thus served as a potential source for the re-peopling of northern latitudes [2]. The post-LGM genetic signature was previously described as a cline from Western HG (WHG) to Eastern HG (EHG), further shaped by later Holocene expansions from the Near East and the North Pontic steppes [3-9]. Western and central Europe were dominated by ancestry associated with the ∼14,000-year-old individual from Villabruna, Italy, which had largely replaced earlier genetic ancestry, represented by 19,000-15,000-year-old individuals associated with the Magdalenian culture [2]. However, little is known about the genetic diversity in southern European refugia, the presence of distinct genetic clusters, and correspondence with geography. Here, we report new genome-wide data from 11 HGs and Neolithic individuals that highlight the late survival of Paleolithic ancestry in Iberia, reported previously in Magdalenian-associated individuals. We show that all Iberian HGs, including the oldest, a ∼19,000-year-old individual from El Mirón in Spain, carry dual ancestry from both Villabruna and the Magdalenian-related individuals. Thus, our results suggest an early connection between two potential refugia, resulting in a genetic ancestry that survived in later Iberian HGs. Our new genomic data from Iberian Early and Middle Neolithic individuals show that the dual Iberian HG genomic legacy pertains in the peninsula, suggesting that expanding farmers mixed with local HGs. VIDEO ABSTRACT.},
}

@article {pmid30875055,
year = {2019},
author = {Hahn, C},
title = {Assembly of Ancient Mitochondrial Genomes Without a Closely Related Reference Sequence.},
journal = {Methods in molecular biology (Clifton, N.J.)},
volume = {1963},
number = {},
pages = {195-213},
doi = {10.1007/978-1-4939-9176-1_18},
pmid = {30875055},
issn = {1940-6029},
abstract = {Recent methodological advances have transformed the field of ancient DNA (aDNA). Basic bioinformatics skills are becoming essential requirements to process and analyze the sheer amounts of data generated by current aDNA studies and in biomedical research in general. This chapter is intended as a practical guide to the assembly of ancient mitochondrial genomes, directly from genomic DNA-derived next-generation sequencing (NGS) data, specifically in the absence of closely related reference genomes. In a hands-on tutorial suitable for readers with little to no prior bioinformatics experience, we reconstruct the mitochondrial genome of a woolly mammoth deposited ~45,000 years ago. We introduce key software tools and outline general strategies for mitogenome assembly, including the critical quality assessment of assembly results without a reference genome.},
}

@article {pmid30875054,
year = {2019},
author = {Renaud, G and Schubert, M and Sawyer, S and Orlando, L},
title = {Authentication and Assessment of Contamination in Ancient DNA.},
journal = {Methods in molecular biology (Clifton, N.J.)},
volume = {1963},
number = {},
pages = {163-194},
doi = {10.1007/978-1-4939-9176-1_17},
pmid = {30875054},
issn = {1940-6029},
abstract = {Contamination from both present-day humans and postmortem microbial sources is a common challenge in ancient DNA studies. Here we present a suite of tools to assist in the assessment of contamination in ancient DNA data sets. These tools perform standard tests of authenticity of ancient DNA data including detecting the presence of postmortem damage signatures in sequence alignments and quantifying the amount of present-day human contamination.},
}

@article {pmid30875053,
year = {2019},
author = {Nichols, RV and Curd, E and Heintzman, PD and Shapiro, B},
title = {Targeted Amplification and Sequencing of Ancient Environmental and Sedimentary DNA.},
journal = {Methods in molecular biology (Clifton, N.J.)},
volume = {1963},
number = {},
pages = {149-161},
doi = {10.1007/978-1-4939-9176-1_16},
pmid = {30875053},
issn = {1940-6029},
abstract = {All organisms release their DNA into the environment through processes such as excretion and the senescence of tissues and limbs. This DNA, often referred to as environmental DNA (eDNA) or sedimentary ancient DNA (sedaDNA), can be recovered from both present-day and ancient soils, fecal samples, bodies of water and lake cores, and even air. While eDNA is a potentially useful record of past and present biodiversity, several challenges complicate data generation and interpretation of results. Most importantly, eDNA samples tend to be highly taxonomically mixed, and the target organism or group of organisms may be present at very low abundance within this mixture. To overcome this challenge, enrichment approaches are often used to target specific taxa of interest. Here, we describe a protocol to amplify metabarcodes or short, variable loci that identify lineages within broad taxonomic groups (e.g., plants, mammals), using the polymerase chain reaction (PCR) with established generic "barcode" primers. We also provide a catalog of animal and plant barcode primers that, because they target relatively short fragments of DNA, are potentially suitable for use with degraded DNA.},
}

@article {pmid30875052,
year = {2019},
author = {Wutke, S and Ludwig, A},
title = {Targeted PCR Amplification and Multiplex Sequencing of Ancient DNA for SNP Analysis.},
journal = {Methods in molecular biology (Clifton, N.J.)},
volume = {1963},
number = {},
pages = {141-147},
doi = {10.1007/978-1-4939-9176-1_15},
pmid = {30875052},
issn = {1940-6029},
abstract = {The analysis of single-nucleotide polymorphisms (SNPs) has proven to be advantageous for addressing variation within samples of highly degraded or low-quality DNA samples. This is because only short fragments need to be amplified to analyze SNPs, and this can be achieved by multiplex PCR. Here, we present a sensitive method for the targeted sequencing of SNP loci that requires only small amounts of template DNA. The approach combines multiplex amplification of very short fragments covering SNP positions followed by sample barcoding and next-generation sequencing. This method allows generation of data from large sample sets of poorly preserved specimens, such as fossil remains, forensic samples, and museum specimens. The approach is cost-effective, rapid, and applicable to forensics, population genetics, and phylogenetic research questions.},
}

@article {pmid30875051,
year = {2019},
author = {Paijmans, JLA and González Fortes, G and Förster, DW},
title = {Application of Solid-State Capture for the Retrieval of Small-to-Medium Sized Target Loci from Ancient DNA.},
journal = {Methods in molecular biology (Clifton, N.J.)},
volume = {1963},
number = {},
pages = {129-139},
doi = {10.1007/978-1-4939-9176-1_14},
pmid = {30875051},
issn = {1940-6029},
abstract = {Genetic studies that include ancient samples are often hampered by the low amount of endogenous DNA that ancient samples often contain, relative to co-extracted "contaminant" DNA from other organisms. One approach to mitigate this challenge is to perform hybridization-based capture of target genomic regions using DNA or RNA baits. Such baits are designed to have high sequence similarity to the target genomic regions and can reduce the off-target fraction in DNA sequencing libraries. Here, we present a protocol to use Agilent SureSelect microarrays to enrich ancient DNA libraries for small-to-medium-sized target loci, such as mitochondrial genomes, from ancient DNA extracts. The protocol that we present builds on previously published work by introducing improvements that improve recovery of short DNA fragments while minimizing the cost and duration of the experiment.},
}

@article {pmid30875050,
year = {2019},
author = {Soares, AER},
title = {Hybridization Capture of Ancient DNA Using RNA Baits.},
journal = {Methods in molecular biology (Clifton, N.J.)},
volume = {1963},
number = {},
pages = {121-128},
doi = {10.1007/978-1-4939-9176-1_13},
pmid = {30875050},
issn = {1940-6029},
abstract = {The majority of DNA recovered from ancient remains is derived from organisms that colonize the remains post-mortem, such as soil microbes, or from contaminants, such as DNA from living humans. Additionally, some ancient DNA research projects aim to target specific genomic regions, such as mitochondrial genomes or variable single nucleotide polymorphisms (SNPs). To overcome the challenge of targeting specific fragments of DNA from within a complex DNA extract, methods have been developed to enrich ancient DNA extracts for target DNA relative to nontarget DNA. This chapter describes a method for target DNA enrichment that uses hybridization to biotinylated RNA baits to capture and amplify specific ancient DNA fragments from within the pool of extracted fragments.},
}

@article {pmid30875049,
year = {2019},
author = {Snyder-Mackler, N and Voyles, T and Tung, J},
title = {Generating RNA Baits for Capture-Based Enrichment.},
journal = {Methods in molecular biology (Clifton, N.J.)},
volume = {1963},
number = {},
pages = {107-120},
doi = {10.1007/978-1-4939-9176-1_12},
pmid = {30875049},
issn = {1940-6029},
abstract = {Capture-based enrichment techniques have revolutionized genomic analysis of species and populations for which only low-quality or contaminated DNA samples (e.g., ancient DNA, noninvasively collected DNA, environmental DNA) are available. This chapter outlines an optimized laboratory protocol for generating RNA "baits" for genome-wide capture of target DNA from a larger pool of DNA. This method relies on the in vitro transcription of biotinylated RNA baits, which has the dual benefit of eliminating the high cost of synthesizing custom baits and producing a bait set that targets the majority of regions genome-wide. We provide a detailed protocol for the three main steps involved in bait library construction: (1) making a DNA library from a high-quality DNA sample for the organism of interest or a closely related species; (2) using duplex-specific nuclease digestion to reduce the representation of repetitive regions in the DNA library; and (3) performing in vitro transcription of the repetitive region-depleted DNA library to generate biotinylated RNA baits. Where applicable, we include notes and recommendations based on our own experiences.},
}

@article {pmid30875048,
year = {2019},
author = {González Fortes, G and Paijmans, JLA},
title = {Whole-Genome Capture of Ancient DNA Using Homemade Baits.},
journal = {Methods in molecular biology (Clifton, N.J.)},
volume = {1963},
number = {},
pages = {93-105},
doi = {10.1007/978-1-4939-9176-1_11},
pmid = {30875048},
issn = {1940-6029},
abstract = {For many archaeological and paleontological samples, the relative content of endogenous compared to contaminant DNA is low. In such cases, enriching sequencing libraries for endogenous DNA, prior to sequencing can make the final research project more cost-effective. Here, we present an in-solution enrichment protocol based on homemade baits that can be applied to recover complete nuclear genomes from ancient remains. The approach is based on the preparation of DNA baits by biotinylated adapter ligation. The procedure has been developed for use with human remains but can be adapted to other species or target regions by choosing the appropriate template DNA from which to build the capture baits. By using homemade rather than commercially acquired baits, this protocol may offer increased flexibility and cost efficiency.},
}

@article {pmid30875046,
year = {2019},
author = {Gansauge, MT and Meyer, M},
title = {A Method for Single-Stranded Ancient DNA Library Preparation.},
journal = {Methods in molecular biology (Clifton, N.J.)},
volume = {1963},
number = {},
pages = {75-83},
doi = {10.1007/978-1-4939-9176-1_9},
pmid = {30875046},
issn = {1940-6029},
abstract = {Genomic library preparation from highly degraded DNA is more efficient when library molecules are prepared separately from the complementary strands of DNA fragments. We describe a protocol in which libraries are constructed from single DNA strands in a three-step procedure: single-stranded ligation of the first adapter with T4 DNA ligase in the presence of a splinter oligonucleotide, copying of the DNA strand with a proofreading polymerase, and blunt-end ligation of the second double-stranded adapter with T4 DNA ligase.},
}

@article {pmid30875045,
year = {2019},
author = {Henneberger, K and Barlow, A and Paijmans, JLA},
title = {Double-Stranded Library Preparation for Ancient and Other Degraded Samples.},
journal = {Methods in molecular biology (Clifton, N.J.)},
volume = {1963},
number = {},
pages = {65-73},
doi = {10.1007/978-1-4939-9176-1_8},
pmid = {30875045},
issn = {1940-6029},
abstract = {High-throughput sequencing (HTS) allows fast and cost-efficient sequencing of ancient DNA (aDNA) without prior information about what sequences should be targeted. One necessary step for HTS is the preparation of a sequencing library. Commercial kits are available for this purpose, but many of these are not suitable for aDNA or other types of damaged DNA. Here, we outline a protocol for HTS library preparation that is optimized for ancient DNA. We report the library conversion rate for a range of input template and adapter concentrations. Our results show that the protocol performs at a high efficiency.},
}

@article {pmid30875044,
year = {2019},
author = {Campos, PF and Gilbert, MTP},
title = {DNA Extraction from Keratin and Chitin.},
journal = {Methods in molecular biology (Clifton, N.J.)},
volume = {1963},
number = {},
pages = {57-63},
doi = {10.1007/978-1-4939-9176-1_7},
pmid = {30875044},
issn = {1940-6029},
abstract = {DNA extracted from keratinous and chitinous materials can be a useful source of genetic information. To effectively liberate the DNA from these materials, buffers containing relatively high levels of DTT, proteinase K, and detergent are recommended, followed by purification using either silica column or organic methods.},
}

@article {pmid30875043,
year = {2019},
author = {Wales, N and Kistler, L},
title = {Extraction of Ancient DNA from Plant Remains.},
journal = {Methods in molecular biology (Clifton, N.J.)},
volume = {1963},
number = {},
pages = {45-55},
doi = {10.1007/978-1-4939-9176-1_6},
pmid = {30875043},
issn = {1940-6029},
abstract = {Ancient plant remains from archaeological sites, paleoenvironmental contexts, and herbaria provide excellent opportunities for interrogating plant genetics over Quaternary timescales using ancient DNA (aDNA)-based analyses. A variety of plant tissues, preserved primarily by desiccation and anaerobic waterlogging, have proven to be viable sources of aDNA. Plant tissues are anatomically and chemically diverse and therefore require optimized DNA extraction approaches. Here, we describe a plant DNA isolation protocol that performs well in most contexts. We include recommendations for optimization to retain the very short DNA fragments that are expected to be preserved in degraded tissues.},
}

@article {pmid30875042,
year = {2019},
author = {Epp, LS and Zimmermann, HH and Stoof-Leichsenring, KR},
title = {Sampling and Extraction of Ancient DNA from Sediments.},
journal = {Methods in molecular biology (Clifton, N.J.)},
volume = {1963},
number = {},
pages = {31-44},
doi = {10.1007/978-1-4939-9176-1_5},
pmid = {30875042},
issn = {1940-6029},
abstract = {Environmental DNA preserved in sediments is rapidly gaining importance as a tool in paleoecology. Sampling procedures for sedimentary ancient DNA (sedaDNA) have to be well planned to ensure clean subsampling of the inside of sediment cores and avoid introducing contamination. Additionally, ancient DNA extraction protocols may need to be optimized for the recovery of DNA from sediments, which may contain inhibitors. Here we describe procedures for subsampling both nonfrozen and frozen sediment cores, and we describe an efficient method for ancient DNA extraction from such samples.},
}