@article {pmid34686848,
year = {2021},
author = {Thompson, T},
title = {Ancient DNA points to origins of modern domestic horses.},
journal = {Nature},
volume = {},
number = {},
pages = {},
pmid = {34686848},
issn = {1476-4687},
}

@article {pmid34679854,
year = {2021},
author = {Passamonti, MM and Somenzi, E and Barbato, M and Chillemi, G and Colli, L and Joost, S and Milanesi, M and Negrini, R and Santini, M and Vajana, E and Williams, JL and Ajmone-Marsan, P},
title = {The Quest for Genes Involved in Adaptation to Climate Change in Ruminant Livestock.},
journal = {Animals : an open access journal from MDPI},
volume = {11},
number = {10},
pages = {},
doi = {10.3390/ani11102833},
pmid = {34679854},
issn = {2076-2615},
support = {INEA/CEF/ICT/A2018/1815462//Connecting European Facility (CEF) Telecommunications/ ; INEA/CEF/ICT/A2020/2373580//Connecting European Facility (CEF) Telecommunications/ ; 677353//European Union's Horizon 2020 Research and Innovation Programme/ ; 2012//European Union's Horizon 2020 Partnership for Research and Innovation in the Mediterranean Area Programme (PRIMA/ ; Project "Landscape 4.0 - food, wellbeing and environment"//Italian Ministry of Education, University and Research/ ; Livestock Environment Opendata, 16.2 - PSRN 2014-2020//Fondo Europeo Agricolo per lo Sviluppo Rurale (FEASR)./ ; },
abstract = {Livestock radiated out from domestication centres to most regions of the world, gradually adapting to diverse environments, from very hot to sub-zero temperatures and from wet and humid conditions to deserts. The climate is changing; generally global temperature is increasing, although there are also more extreme cold periods, storms, and higher solar radiation. These changes impact livestock welfare and productivity. This review describes advances in the methodology for studying livestock genomes and the impact of the environment on animal production, giving examples of discoveries made. Sequencing livestock genomes has facilitated genome-wide association studies to localize genes controlling many traits, and population genetics has identified genomic regions under selection or introgressed from one breed into another to improve production or facilitate adaptation. Landscape genomics, which combines global positioning and genomics, has identified genomic features that enable animals to adapt to local environments. Combining the advances in genomics and methods for predicting changes in climate is generating an explosion of data which calls for innovations in the way big data sets are treated. Artificial intelligence and machine learning are now being used to study the interactions between the genome and the environment to identify historic effects on the genome and to model future scenarios.},
}

@article {pmid34663498,
year = {2021},
author = {Calvignac-Spencer, S and Düx, A and Gogarten, JF and Patrono, LV},
title = {Molecular archeology of human viruses.},
journal = {Advances in virus research},
volume = {111},
number = {},
pages = {31-61},
doi = {10.1016/bs.aivir.2021.07.002},
pmid = {34663498},
issn = {1557-8399},
abstract = {The evolution of human-virus associations is usually reconstructed from contemporary patterns of genomic diversity. An intriguing, though still rarely implemented, alternative is to search for the genetic material of viruses in archeological and medical archive specimens to document evolution as it happened. In this chapter, we present lessons from ancient DNA research and incorporate insights from virology to explore the potential range of applications and likely limitations of archeovirological approaches. We also highlight the numerous questions archeovirology will hopefully allow us to tackle in the near future, and the main expected roadblocks to these avenues of research.},
}

@article {pmid34659342,
year = {2021},
author = {Bini, C and Cilli, E and Sarno, S and Traversari, M and Fontani, F and Boattini, A and Pelotti, S and Luiselli, D},
title = {Twenty-Seven Y-Chromosome Short Tandem Repeats Analysis of Italian Mummies of the 16th and 18th Centuries: An Interdisciplinary Research.},
journal = {Frontiers in genetics},
volume = {12},
number = {},
pages = {720640},
doi = {10.3389/fgene.2021.720640},
pmid = {34659342},
issn = {1664-8021},
abstract = {Roccapelago (MO) is a small village located in the Northern Central Apennines, with a population of 31 inhabitants (2014). In 2010, more than 400 individuals dated between the end of the 16th and the 18th century, many of which partially mummified, were discovered in the crypt of the church. This small village, because of its geographical location and surrounding environment, seems to possess the characteristics of a genetic isolate, useful for population genetics and genealogical analyses. Thus, a diachronic study of DNA aimed at investigating the structure and dynamics of the population of Roccapelago over the about 4 centuries, was conducted by analyzing ancient and modern inhabitants of the village. The 14 modern samples were selected by considering both the founder surnames of the village, identified thanks to the study of parish registers, and the grandparent's criterion. From 25 ancient mummies, morphologically assigned to male individuals, the petrous bone, that harbors high DNA amounts, was selected for the DNA extraction. The quantification and qualitative assessment of total human male DNA were evaluated by a real-time PCR assay using the Quantifiler Trio DNA Quantification Kit and multiplex PCR of 27 Y-chromosome short tandem repeat (Y-STR) markers included in the Yfiler Plus PCR Amplification Kit, with seven rapidly mutating Y-STR loci for improving discrimination of male lineages, was performed to genotype the samples. Y-STRs were analyzed according to the criteria of ancient DNA (aDNA) analysis to ensure that authentic DNA typing results were obtained from these ancient samples. The molecular analysis showed the usefulness of the Y chromosome to identify historically relevant remains and discover patterns of relatedness in communities moving from anthropology to genetic genealogy and forensics.},
}

@article {pmid34656830,
year = {2021},
author = {Wurst, C and Maixner, F and Castella, V and Cipollini, G and Hotz, G and Zink, A},
title = {The Lady from Basel's Barfüsserkirche - Molecular confirmation of the Mummy's identity through mitochondrial DNA of living relatives spanning 22 generations.},
journal = {Forensic science international. Genetics},
volume = {56},
number = {},
pages = {102604},
doi = {10.1016/j.fsigen.2021.102604},
pmid = {34656830},
issn = {1878-0326},
abstract = {The identity of the mummified Lady from the Barfüsser Church in Basel, Switzerland has been unsolved for decades, despite the prominent location of the burial place in front of the choir screen. A recent multidisciplinary research approach came up with a possible candidate, Anna Catharina Bischoff who died in Basel in 1787 with an age of 69 years (1719-1787). To verify the identity of the mummy, genealogists of the Citizen Science Basel discovered three living individuals of the maternal lineage of two different family branches, separated from Anna Catharina Bischoff by up to 22 generations. In this study we compare the ancient mitochondrial DNA of the mummy recovered from a premolar to the mitochondrial DNA of these three candidates. Initially the mitochondrial hypervariable regions I and II of the living individuals were screened using the Sanger sequencing method. This was followed by a mitochondrial capture approach and next generation sequencing to enrich for the whole mitochondrial genome of the mummy and one living person. A full mitochondrial genome has been recovered of both individuals sharing an identical haplotype. The sequence was assigned to the mitochondrial haplogroup U5a1+!16192 including two private mutations 10006G and 16293C. Only by using an interdisciplinary approach combining ancient DNA analysis and genealogy a maternal lineage of a non-noble family spanning 22 generations could be confirmed.},
}

@article {pmid34655517,
year = {2021},
author = {Ciucani, MM and Jensen, JK and Sinding, MS and Smith, O and Lucenti, SB and Rosengren, E and Rook, L and Tuveri, C and Arca, M and Cappellini, E and Galaverni, M and Randi, E and Guo, C and Zhang, G and Sicheritz-Pontén, T and Dalén, L and Gilbert, MTP and Gopalakrishnan, S},
title = {Evolutionary history of the extinct Sardinian dhole.},
journal = {Current biology : CB},
volume = {},
number = {},
pages = {},
doi = {10.1016/j.cub.2021.09.059},
pmid = {34655517},
issn = {1879-0445},
abstract = {The Sardinian dhole (Cynotherium sardous)1 was an iconic and unique canid species that was endemic to Sardinia and Corsica until it became extinct at the end of the Late Pleistocene.2-5 Given its peculiar dental morphology, small body size, and high level of endemism, several extant canids have been proposed as possible relatives of the Sardinian dhole, including the Asian dhole and African hunting dog ancestor.3,6-9 Morphometric analyses3,6,8-12 have failed to clarify the evolutionary relationship with other canids.We sequenced the genome of a ca-21,100-year-old Sardinian dhole in order to understand its genomic history and clarify its phylogenetic position. We found that it represents a separate taxon from all other living canids from Eurasia, Africa, and North America, and that the Sardinian dhole lineage diverged from the Asian dhole ca 885 ka. We additionally detected historical gene flow between the Sardinian and Asian dhole lineages, which ended approximately 500-300 ka, when the land bridge between Sardinia and mainland Italy was already broken, severing their population connectivity. Our sample showed low genome-wide diversity compared to other extant canids-probably a result of the long-term isolation-that could have contributed to the subsequent extinction of the Sardinian dhole.},
}

@article {pmid34643070,
year = {2021},
author = {Boubli, JP and Janiak, MC and Porter, LM and Torre, S and Cortés-Ortiz, L and Da Silva, MNF and Rylands, AB and Nash, S and Bertuol, F and Byrne, H and Silva, FE and Rohe, F and Vries, D and Beck, RMD and Ruiz-Gartzia, I and Kuderna, LFK and Marques-Bonet, T and Hrbek, T and Farias, IP and Van Heteren, AH and Roos, C},
title = {Ancient DNA of the pygmy marmoset type specimen Cebuella pygmaea (Spix, 1823) resolves a taxonomic conundrum.},
journal = {Zoological research},
volume = {42},
number = {6},
pages = {761-771},
doi = {10.24272/j.issn.2095-8137.2021.143},
pmid = {34643070},
issn = {2095-8137},
abstract = {The pygmy marmoset, the smallest of the anthropoid primates, has a broad distribution in Western Amazonia. Recent studies using molecular and morphological data have identified two distinct species separated by the Napo and Solimões-Amazonas rivers. However, reconciling this new biological evidence with current taxonomy, i.e., two subspecies, Cebuella pygmaea pygmaea (Spix, 1823) and Cebuella pygmaea niveiventris (Lönnberg, 1940), was problematic given the uncertainty as to whether Spix's pygmy marmoset (Cebuella pygmaea pygmaea) was collected north or south of the Napo and Solimões-Amazonas rivers, making it unclear to which of the two newly revealed species the name pygmaea would apply. Here, we present the first molecular data from Spix's type specimen of Cebuella pygmaea, as well as novel mitochondrial genomes from modern pygmy marmosets sampled near the type locality (Tabatinga) on both sides of the river. With these data, we can confirm the correct names of the two species identified, i.e., C. pygmaea for animals north of the Napo and Solimões-Amazonas rivers and C. niveiventris for animals south of these two rivers. Phylogenetic analyses of the novel genetic data placed into the context of cytochrome b gene sequences from across the range of pygmy marmosets further led us to re-evaluate the geographical distribution for the two Cebuella species. We dated the split of these two species to 2.54 million years ago. We discuss additional, more recent, subdivisions within each lineage, as well as potential contact zones between the two species in the headwaters of these rivers.},
}

@article {pmid34630530,
year = {2021},
author = {Ning, C and Zheng, HX and Zhang, F and Wu, S and Li, C and Zhao, Y and Xu, Y and Wei, D and Wu, Y and Gao, S and Jin, L and Cui, Y},
title = {Ancient Mitochondrial Genomes Reveal Extensive Genetic Influence of the Steppe Pastoralists in Western Xinjiang.},
journal = {Frontiers in genetics},
volume = {12},
number = {},
pages = {740167},
doi = {10.3389/fgene.2021.740167},
pmid = {34630530},
issn = {1664-8021},
abstract = {The population prehistory of Xinjiang has been a hot topic among geneticists, linguists, and archaeologists. Current ancient DNA studies in Xinjiang exclusively suggest an admixture model for the populations in Xinjiang since the early Bronze Age. However, almost all of these studies focused on the northern and eastern parts of Xinjiang; the prehistoric demographic processes that occurred in western Xinjiang have been seldomly reported. By analyzing complete mitochondrial sequences from the Xiabandi (XBD) cemetery (3,500-3,300 BP), the up-to-date earliest cemetery excavated in western Xinjiang, we show that all the XBD mitochondrial sequences fall within two different West Eurasian mitochondrial DNA (mtDNA) pools, indicating that the migrants into western Xinjiang from west Eurasians were a consequence of the early expansion of the middle and late Bronze Age steppe pastoralists (Steppe_MLBA), admixed with the indigenous populations from Central Asia. Our study provides genetic links for an early existence of the Indo-Iranian language in southwestern Xinjiang and suggests that the existence of Andronovo culture in western Xinjiang involved not only the dispersal of ideas but also population movement.},
}

@article {pmid34618559,
year = {2021},
author = {Kocher, A and Papac, L and Barquera, R and Key, FM and Spyrou, MA and Hübler, R and Rohrlach, AB and Aron, F and Stahl, R and Wissgott, A and van Bömmel, F and Pfefferkorn, M and Mittnik, A and Villalba-Mouco, V and Neumann, GU and Rivollat, M and van de Loosdrecht, MS and Majander, K and Tukhbatova, RI and Musralina, L and Ghalichi, A and Penske, S and Sabin, S and Michel, M and Gretzinger, J and Nelson, EA and Ferraz, T and Nägele, K and Parker, C and Keller, M and Guevara, EK and Feldman, M and Eisenmann, S and Skourtanioti, E and Giffin, K and Gnecchi-Ruscone, GA and Friederich, S and Schimmenti, V and Khartanovich, V and Karapetian, MK and Chaplygin, MS and Kufterin, VV and Khokhlov, AA and Chizhevsky, AA and Stashenkov, DA and Kochkina, AF and Tejedor-Rodríguez, C and de Lagrán, ÍG and Arcusa-Magallón, H and Garrido-Pena, R and Royo-Guillén, JI and Nováček, J and Rottier, S and Kacki, S and Saintot, S and Kaverzneva, E and Belinskiy, AB and Velemínský, P and Limburský, P and Kostka, M and Loe, L and Popescu, E and Clarke, R and Lyons, A and Mortimer, R and Sajantila, A and de Armas, YC and Hernandez Godoy, ST and Hernández-Zaragoza, DI and Pearson, J and Binder, D and Lefranc, P and Kantorovich, AR and Maslov, VE and Lai, L and Zoledziewska, M and Beckett, JF and Langová, M and Danielisová, A and Ingman, T and Atiénzar, GG and de Miguel Ibáñez, MP and Romero, A and Sperduti, A and Beckett, S and Salter, SJ and Zilivinskaya, ED and Vasil'ev, DV and von Heyking, K and Burger, RL and Salazar, LC and Amkreutz, L and Navruzbekov, M and Rosenstock, E and Alonso-Fernández, C and Slavchev, V and Kalmykov, AA and Atabiev, BC and Batieva, E and Calmet, MA and Llamas, B and Schultz, M and Krauß, R and Jiménez-Echevarría, J and Francken, M and Shnaider, S and de Knijff, P and Altena, E and Van de Vijver, K and Fehren-Schmitz, L and Tung, TA and Lösch, S and Dobrovolskaya, M and Makarov, N and Read, C and Van Twest, M and Sagona, C and Ramsl, PC and Akar, M and Yener, KA and Ballestero, EC and Cucca, F and Mazzarello, V and Utrilla, P and Rademaker, K and Fernández-Domínguez, E and Baird, D and Semal, P and Márquez-Morfín, L and Roksandic, M and Steiner, H and Salazar-García, DC and Shishlina, N and Erdal, YS and Hallgren, F and Boyadzhiev, Y and Boyadzhiev, K and Küßner, M and Sayer, D and Onkamo, P and Skeates, R and Rojo-Guerra, M and Buzhilova, A and Khussainova, E and Djansugurova, LB and Beisenov, AZ and Samashev, Z and Massy, K and Mannino, M and Moiseyev, V and Mannermaa, K and Balanovsky, O and Deguilloux, MF and Reinhold, S and Hansen, S and Kitov, EP and Dobeš, M and Ernée, M and Meller, H and Alt, KW and Prüfer, K and Warinner, C and Schiffels, S and Stockhammer, PW and Bos, K and Posth, C and Herbig, A and Haak, W and Krause, J and Kühnert, D},
title = {Ten millennia of hepatitis B virus evolution.},
journal = {Science (New York, N.Y.)},
volume = {374},
number = {6564},
pages = {182-188},
doi = {10.1126/science.abi5658},
pmid = {34618559},
issn = {1095-9203},
abstract = {[Figure: see text].},
}

@article {pmid34616690,
year = {2021},
author = {Shiba, T and Komatsu, K and Sudo, T and Sawafuji, R and Saso, A and Ueda, S and Watanabe, T and Nemoto, T and Kano, C and Nagai, T and Ohsugi, Y and Katagiri, S and Takeuchi, Y and Kobayashi, H and Iwata, T},
title = {Comparison of Periodontal Bacteria of Edo and Modern Periods Using Novel Diagnostic Approach for Periodontitis With Micro-CT.},
journal = {Frontiers in cellular and infection microbiology},
volume = {11},
number = {},
pages = {723821},
doi = {10.3389/fcimb.2021.723821},
pmid = {34616690},
issn = {2235-2988},
abstract = {Ancient dental calculus, formed from dental plaque, is a rich source of ancient DNA and can provide information regarding the food and oral microbiology at that time. Genomic analysis of dental calculus from Neanderthals has revealed the difference in bacterial composition of oral microbiome between Neanderthals and modern humans. There are few reports investigating whether the pathogenic bacteria of periodontitis, a polymicrobial disease induced in response to the accumulation of dental plaque, were different between ancient and modern humans. This study aimed to compare the bacterial composition of the oral microbiome in ancient and modern human samples and to investigate whether lifestyle differences depending on the era have altered the bacterial composition of the oral microbiome and the causative bacteria of periodontitis. Additionally, we introduce a novel diagnostic approach for periodontitis in ancient skeletons using micro-computed tomography. Ancient 16S rDNA sequences were obtained from 12 samples at the Unko-in site (18th-19th century) of the Edo era (1603-1867), a characteristic period in Japan when immigrants were not accepted. Furthermore, modern 16S rDNA data from 53 samples were obtained from a database to compare the modern and ancient microbiome. The microbial co-occurrence network was analyzed based on 16S rDNA read abundance. Eubacterium species, Mollicutes species, and Treponema socranskii were the core species in the Edo co-occurrence network. The co-occurrence relationship between Actinomyces oricola and Eggerthella lenta appeared to have played a key role in causing periodontitis in the Edo era. However, Porphyromonas gingivalis, Fusobacterium nucleatum subsp. vincentii, and Prevotella pleuritidis were the core and highly abundant species in the co-occurrence network of modern samples. These results suggest the possibility of differences in the pathogens causing periodontitis during different eras in history.},
}

@article {pmid33945536,
year = {2021},
author = {Nerlich, AG and Kirchhoff, SM and Panzer, S and Lehn, C and Bachmeier, BE and Bayer, B and Anslinger, K and Röcker, P and Peschel, OK},
title = {Chronic active non-lethal human-type tuberculosis in a high royal Bavarian officer of Napoleonic times-a mummy study.},
journal = {PloS one},
volume = {16},
number = {5},
pages = {e0249955},
pmid = {33945536},
issn = {1932-6203},
mesh = {DNA, Ancient/chemistry ; Humans ; Male ; Mummies/diagnostic imaging/microbiology/*pathology ; Spine/diagnostic imaging/pathology ; Tomography, X-Ray Computed ; Tuberculosis/diagnostic imaging/microbiology/*pathology ; },
abstract = {In paleopathology, morphological and molecular evidence for infection by mycobacteria of the M. tuberculosis complex (MTC) is frequently associated with early death. In the present report, we describe a multidisciplinary study of a well-preserved mummy from Napoleonic times with a long-standing tuberculous infection by M. tuberculosis senso stricto who died at the age of 88 years of focal and non-MTB related bronchopneumonia. The well-preserved natural mummy of the Royal Bavarian General, Count Heinrich LII Reuss-Köstritz (1763-1851 CE), was extensively investigated by macro- and histomorphology, whole body CT scans and organ radiography, various molecular tissue analyses, including stable isotope analysis and molecular genetic tests. We identified signs for a long-standing, but terminally inactive pulmonary tuberculosis, tuberculous destruction of the second lumbar vertebral body, and a large tuberculous abscess in the right (retroperitoneal) psoas region (a cold abscess). This cold abscess harboured an active tuberculous infection as evidenced by histological and molecular tests. Radiological and histological analysis further revealed extensive arteriosclerosis with (non-obliterating) coronary and significant carotid arteriosclerosis, healthy bone tissue without evidence of age-related osteopenia, evidence for diffuse idiopathic skeletal hyperostosis and mild osteoarthrosis of few joints. This suggests excellent living conditions correlating well with his diet indicated by stable isotope results and literary evidence. Despite the clear evidence of a tuberculous cold abscess with bacterioscopic and molecular proof for a persisting MTC infection of a human-type M. tuberculosis strain, we can exclude the chronic MTC infection as cause of death. The detection of MTC in historic individuals should therefore be interpreted with great caution and include further data, such as their nutritional status.},
}

DNA, Ancient/chemistry
Humans
Male
Mummies/diagnostic imaging/microbiology/*pathology
Spine/diagnostic imaging/pathology
Tomography, X-Ray Computed
Tuberculosis/diagnostic imaging/microbiology/*pathology

@article {pmid34610848,
year = {2021},
author = {Pfrengle, S and Neukamm, J and Guellil, M and Keller, M and Molak, M and Avanzi, C and Kushniarevich, A and Montes, N and Neumann, GU and Reiter, E and Tukhbatova, RI and Berezina, NY and Buzhilova, AP and Korobov, DS and Suppersberger Hamre, S and Matos, VMJ and Ferreira, MT and González-Garrido, L and Wasterlain, SN and Lopes, C and Santos, AL and Antunes-Ferreira, N and Duarte, V and Silva, AM and Melo, L and Sarkic, N and Saag, L and Tambets, K and Busso, P and Cole, ST and Avlasovich, A and Roberts, CA and Sheridan, A and Cessford, C and Robb, J and Krause, J and Scheib, CL and Inskip, SA and Schuenemann, VJ},
title = {Mycobacterium leprae diversity and population dynamics in medieval Europe from novel ancient genomes.},
journal = {BMC biology},
volume = {19},
number = {1},
pages = {220},
pmid = {34610848},
issn = {1741-7007},
support = {No. 2014-2020.4.01.16-0030//European Regional Development Fund/ ; PUT1339//Estonian Research Council personal research grants/ ; PRG243//Estonian Research Council personal research grants/ ; PRG1027//Research Council personal research grants/ ; 2000368/Z/15/Z/WT_/Wellcome Trust/United Kingdom ; 2018/31/B/HS3/01464//National Science Centre in Poland/ ; 845479//H2020 Marie Skłodowska-Curie Actions/ ; UIDB/00283/2020//Fundação para a Ciência e a Tecnologia/ ; IF001862014//Fundação para a Ciência e a Tecnologia/ ; SFRH/BD/130165/2017//Fundação para a Ciência e a Tecnologia/ ; UIDB/04004/2020//Fundação para a Ciência e a Tecnologia/ ; SFRH/BPD/117128/2016//Fundação para a Ciência e a Tecnologia/ ; },
abstract = {BACKGROUND: Hansen's disease (leprosy), widespread in medieval Europe, is today mainly prevalent in tropical and subtropical regions with around 200,000 new cases reported annually. Despite its long history and appearance in historical records, its origins and past dissemination patterns are still widely unknown. Applying ancient DNA approaches to its major causative agent, Mycobacterium leprae, can significantly improve our understanding of the disease's complex history. Previous studies have identified a high genetic continuity of the pathogen over the last 1500 years and the existence of at least four M. leprae lineages in some parts of Europe since the Early Medieval period.

RESULTS: Here, we reconstructed 19 ancient M. leprae genomes to further investigate M. leprae's genetic variation in Europe, with a dedicated focus on bacterial genomes from previously unstudied regions (Belarus, Iberia, Russia, Scotland), from multiple sites in a single region (Cambridgeshire, England), and from two Iberian leprosaria. Overall, our data confirm the existence of similar phylogeographic patterns across Europe, including high diversity in leprosaria. Further, we identified a new genotype in Belarus. By doubling the number of complete ancient M. leprae genomes, our results improve our knowledge of the past phylogeography of M. leprae and reveal a particularly high M. leprae diversity in European medieval leprosaria.

CONCLUSIONS: Our findings allow us to detect similar patterns of strain diversity across Europe with branch 3 as the most common branch and the leprosaria as centers for high diversity. The higher resolution of our phylogeny tree also refined our understanding of the interspecies transfer between red squirrels and humans pointing to a late antique/early medieval transmission. Furthermore, with our new estimates on the past population diversity of M. leprae, we gained first insights into the disease's global history in relation to major historic events such as the Roman expansion or the beginning of the regular transatlantic long distance trade. In summary, our findings highlight how studying ancient M. leprae genomes worldwide improves our understanding of leprosy's global history and can contribute to current models of M. leprae's worldwide dissemination, including interspecies transmissions.},
}

@article {pmid34521829,
year = {2021},
author = {Klein, CG and Pisani, D and Field, DJ and Lakin, R and Wills, MA and Longrich, NR},
title = {Evolution and dispersal of snakes across the Cretaceous-Paleogene mass extinction.},
journal = {Nature communications},
volume = {12},
number = {1},
pages = {5335},
pmid = {34521829},
issn = {2041-1723},
mesh = {Amphibians ; Animal Distribution/*physiology ; Animals ; Biodiversity ; Birds ; DNA, Ancient/analysis ; *Extinction, Biological ; Fishes ; Fossils/history ; *Genetic Speciation ; History, Ancient ; Mammals ; *Phylogeny ; Phylogeography ; Snakes/anatomy & histology/*classification/genetics ; },
abstract = {Mass extinctions have repeatedly shaped global biodiversity. The Cretaceous-Paleogene (K-Pg) mass extinction caused the demise of numerous vertebrate groups, and its aftermath saw the rapid diversification of surviving mammals, birds, frogs, and teleost fishes. However, the effects of the K-Pg extinction on the evolution of snakes-a major clade of predators comprising over 3,700 living species-remains poorly understood. Here, we combine an extensive molecular dataset with phylogenetically and stratigraphically constrained fossil calibrations to infer an evolutionary timescale for Serpentes. We reveal a potential diversification among crown snakes associated with the K-Pg mass extinction, led by the successful colonisation of Asia by the major extant clade Afrophidia. Vertebral morphometrics suggest increasing morphological specialisation among marine snakes through the Paleogene. The dispersal patterns of snakes following the K-Pg underscore the importance of this mass extinction event in shaping Earth's extant vertebrate faunas.},
}

Amphibians
Animal Distribution/*physiology
Animals
Biodiversity
Birds
DNA, Ancient/analysis
*Extinction, Biological
Fishes
Fossils/history
*Genetic Speciation
History, Ancient
Mammals
*Phylogeny
Phylogeography
Snakes/anatomy & histology/*classification/genetics

@article {pmid34493715,
year = {2021},
author = {Coll Macià, M and Skov, L and Peter, BM and Schierup, MH},
title = {Different historical generation intervals in human populations inferred from Neanderthal fragment lengths and mutation signatures.},
journal = {Nature communications},
volume = {12},
number = {1},
pages = {5317},
pmid = {34493715},
issn = {2041-1723},
mesh = {Animals ; Asia ; Crosses, Genetic ; DNA, Ancient/*analysis ; Europe ; Female ; *Gene Flow ; *Genome, Human ; History, 21st Century ; History, Ancient ; Humans ; Male ; *Mutation ; Neanderthals/*genetics ; Polymorphism, Restriction Fragment Length ; },
abstract = {After the main Out-of-Africa event, humans interbred with Neanderthals leaving 1-2% of Neanderthal DNA scattered in small fragments in all non-African genomes today. Here we investigate what can be learned about human demographic processes from the size distribution of these fragments. We observe differences in fragment length across Eurasia with 12% longer fragments in East Asians than West Eurasians. Comparisons between extant populations with ancient samples show that these differences are caused by different rates of decay in length by recombination since the Neanderthal admixture. In concordance, we observe a strong correlation between the average fragment length and the mutation accumulation, similar to what is expected by changing the ages at reproduction as estimated from trio studies. Altogether, our results suggest differences in the generation interval across Eurasia, by up 10-20%, over the past 40,000 years. We use sex-specific mutation signatures to infer whether these changes were driven by shifts in either male or female age at reproduction, or both. We also find that previously reported variation in the mutational spectrum may be largely explained by changes to the generation interval. We conclude that Neanderthal fragment lengths provide unique insight into differences among human populations over recent history.},
}

Animals
Asia
Crosses, Genetic
DNA, Ancient/*analysis
Europe
Female
*Gene Flow
*Genome, Human
History, 21st Century
History, Ancient
Humans
Male
*Mutation
Neanderthals/*genetics
Polymorphism, Restriction Fragment Length

@article {pmid34601464,
year = {2021},
author = {Scheib, CL},
title = {Ancient Health Landscape: foundations and perspectives.},
journal = {Journal of anthropological sciences = Rivista di antropologia : JASS},
volume = {99},
number = {},
pages = {},
doi = {10.4436/JASS.99011},
pmid = {34601464},
issn = {2037-0644},
}

@article {pmid34601461,
year = {2021},
author = {Hellenthal, G},
title = {Methodological challenges and opportunities for inferring human demography.},
journal = {Journal of anthropological sciences = Rivista di antropologia : JASS},
volume = {99},
number = {},
pages = {},
doi = {10.4436/JASS.99010},
pmid = {34601461},
issn = {2037-0644},
}

@article {pmid34601460,
year = {2021},
author = {Bergström, A},
title = {From domestication genomics towards molecular ecology of human environments.},
journal = {Journal of anthropological sciences = Rivista di antropologia : JASS},
volume = {99},
number = {},
pages = {},
doi = {10.4436/JASS.99007},
pmid = {34601460},
issn = {2037-0644},
}

@article {pmid34593021,
year = {2021},
author = {Granehäll, L and Huang, KD and Tett, A and Manghi, P and Paladin, A and O'Sullivan, N and Rota-Stabelli, O and Segata, N and Zink, A and Maixner, F},
title = {Metagenomic analysis of ancient dental calculus reveals unexplored diversity of oral archaeal Methanobrevibacter.},
journal = {Microbiome},
volume = {9},
number = {1},
pages = {197},
pmid = {34593021},
issn = {2049-2618},
abstract = {BACKGROUND: Dental calculus (mineralised dental plaque) preserves many types of microfossils and biomolecules, including microbial and host DNA, and ancient calculus are thus an important source of information regarding our ancestral human oral microbiome. In this study, we taxonomically characterised the dental calculus microbiome from 20 ancient human skeletal remains originating from Trentino-South Tyrol, Italy, dating from the Neolithic (6000-3500 BCE) to the Early Middle Ages (400-1000 CE).

RESULTS: We found a high abundance of the archaeal genus Methanobrevibacter in the calculus. However, only a fraction of the sequences showed high similarity to Methanobrevibacter oralis, the only described Methanobrevibacter species in the human oral microbiome so far. To further investigate the diversity of this genus, we used de novo metagenome assembly to reconstruct 11 Methanobrevibacter genomes from the ancient calculus samples. Besides the presence of M. oralis in one of the samples, our phylogenetic analysis revealed two hitherto uncharacterised and unnamed oral Methanobrevibacter species that are prevalent in ancient calculus samples sampled from a broad range of geographical locations and time periods.

CONCLUSIONS: We have shown the potential of using de novo metagenomic assembly on ancient samples to explore microbial diversity and evolution. Our study suggests that there has been a possible shift in the human oral microbiome member Methanobrevibacter over the last millennia. Video abstract.},
}

@article {pmid34554811,
year = {2021},
author = {Liu, Y and Mao, X and Krause, J and Fu, Q},
title = {Insights into human history from the first decade of ancient human genomics.},
journal = {Science (New York, N.Y.)},
volume = {373},
number = {6562},
pages = {1479-1484},
doi = {10.1126/science.abi8202},
pmid = {34554811},
issn = {1095-9203},
mesh = {Africa ; Americas ; Animals ; Asia ; Biological Evolution ; *DNA, Ancient ; Europe ; *Genome, Human ; *Genomics ; History, Ancient ; Human Migration ; Humans ; Neanderthals/genetics ; Oceania ; *Population Dynamics ; Siberia ; },
abstract = {[Figure: see text].},
}

Africa
Americas
Animals
Asia
Biological Evolution
*DNA, Ancient
Europe
*Genome, Human
*Genomics
History, Ancient
Human Migration
Humans
Neanderthals/genetics
Oceania
*Population Dynamics
Siberia

@article {pmid34410492,
year = {2021},
author = {Aneli, S and Caldon, M and Saupe, T and Montinaro, F and Pagani, L},
title = {Through 40,000 years of human presence in Southern Europe: the Italian case study.},
journal = {Human genetics},
volume = {140},
number = {10},
pages = {1417-1431},
pmid = {34410492},
issn = {1432-1203},
mesh = {DNA, Ancient/*analysis ; European Continental Ancestry Group/*genetics/*history ; *Evolution, Molecular ; *Genetic Variation ; *Genome, Human ; Genomics/*history ; History, Ancient ; History, Medieval ; Humans ; Italy ; },
abstract = {The Italian Peninsula, a natural pier across the Mediterranean Sea, witnessed intricate population events since the very beginning of the human occupation in Europe. In the last few years, an increasing number of modern and ancient genomes from the area have been published by the international research community. This genomic perspective started unveiling the relevance of Italy to understand the post-Last Glacial Maximum (LGM) re-peopling of Europe, the earlier phase of the Neolithic westward migrations, and its linking role between Eastern and Western Mediterranean areas after the Iron Age. However, many open questions are still waiting for more data to be addressed in full. With this review, we summarize the current knowledge emerging from the available ancient Italian individuals and, by re-analysing them all at once, we try to shed light on the avenues future research in the area should cover. In particular, open questions concern (1) the fate of pre-Villabruna Europeans and to what extent their genomic components were absorbed by the post-LGM hunter-gatherers; (2) the role of Sicily and Sardinia before LGM; (3) to what degree the documented genetic structure within the Early Neolithic settlers can be described as two separate migrations; (4) what are the population events behind the marked presence of an Iranian Neolithic-like component in Bronze Age and Iron Age Italian and Southern European samples.},
}

DNA, Ancient/*analysis
European Continental Ancestry Group/*genetics/*history
*Evolution, Molecular
*Genetic Variation
*Genome, Human
Genomics/*history
History, Ancient
History, Medieval
Humans
Italy

@article {pmid34582623,
year = {2021},
author = {Suchan, T and Kusliy, MA and Khan, N and Chauvey, L and Tonasso-Calvière, L and Schiavinato, S and Southon, J and Keller, M and Kitagawa, K and Krause, J and Bessudnov, AN and Bessudnov, AA and Graphodatsky, AS and Lamas, SV and Wilczyński, J and Pospuła, S and Tunia, K and Nowak, M and Moskal-delHoyo, M and Tishkin, AA and Pryor, AJE and Outram, AK and Orlando, L},
title = {Performance and automation of ancient DNA capture with RNA hyRAD probes.},
journal = {Molecular ecology resources},
volume = {},
number = {},
pages = {},
doi = {10.1111/1755-0998.13518},
pmid = {34582623},
issn = {1755-0998},
abstract = {DNA hybridization-capture techniques allow researchers to focus their sequencing efforts on pre-selected genomic regions. This feature is especially useful when analyzing ancient DNA (aDNA) extracts, which are often dominated by exogenous environmental sources. Here, we assessed, for the first time, the performance of hyRAD as an inexpensive and design-free alternative to commercial capture protocols to obtain authentic aDNA data from osseous remains. HyRAD relies on double enzymatic restriction of fresh DNA extracts to produce RNA probes that cover only a fraction of the genome and can serve as baits for capturing homologous fragments from aDNA libraries. We found that this approach could retrieve sequence data from horse remains coming from a range of preservation environments, including beyond radiocarbon range, yielding up to 146.5-fold on-target enrichment for aDNA extracts showing extremely low endogenous content (<1%). Performance was, however, more limited for those samples already characterized by good DNA preservation (>20-30%), while the fraction of endogenous reads mapping on- and off-target was relatively insensitive to the original endogenous DNA content. Procedures based on two, instead of a single round of capture, increased on-target coverage up to 3.6-fold. Additionally, we used methylation sensitive restriction enzymes to produce probes targeting hypomethylated regions, which improved data quality by reducing post-mortem DNA damage and mapping within multicopy regions. Finally, we developed a fully automated hyRAD protocol leveraging inexpensive robotic platforms to facilitate capture processing. Overall, our work establishes hyRAD as a cost-effective strategy to recover a set of shared orthologous variants across multiple ancient samples.},
}

@article {pmid34573393,
year = {2021},
author = {Vai, S and Lari, M and Caramelli, D},
title = {Ancient and Archaic Genomes.},
journal = {Genes},
volume = {12},
number = {9},
pages = {},
doi = {10.3390/genes12091411},
pmid = {34573393},
issn = {2073-4425},
abstract = {The first data obtained from ancient DNA samples were published more than thirty years ago [...].},
}

@article {pmid34569945,
year = {2021},
author = {Rotival, M and Quintana-Murci, L},
title = {Towards a functional understanding of adaptive phenotypes in humans.},
journal = {Journal of anthropological sciences = Rivista di antropologia : JASS},
volume = {99},
number = {},
pages = {},
doi = {10.4436/JASS.99006},
pmid = {34569945},
issn = {2037-0644},
}

@article {pmid34191029,
year = {2021},
author = {Pérez-Escobar, OA and Bellot, S and Przelomska, NAS and Flowers, JM and Nesbitt, M and Ryan, P and Gutaker, RM and Gros-Balthazard, M and Wells, T and Kuhnhäuser, BG and Schley, R and Bogarín, D and Dodsworth, S and Diaz, R and Lehmann, M and Petoe, P and Eiserhardt, WL and Preick, M and Hofreiter, M and Hajdas, I and Purugganan, M and Antonelli, A and Gravendeel, B and Leitch, IJ and Jimenez, MFT and Papadopulos, AST and Chomicki, G and Renner, SS and Baker, WJ},
title = {Molecular Clocks and Archeogenomics of a Late Period Egyptian Date Palm Leaf Reveal Introgression from Wild Relatives and Add Timestamps on the Domestication.},
journal = {Molecular biology and evolution},
volume = {38},
number = {10},
pages = {4475-4492},
doi = {10.1093/molbev/msab188},
pmid = {34191029},
issn = {1537-1719},
support = {//Swedish Research Council/ ; //Swedish Foundation/ ; //Knut and Alice Wallenberg Foundation/ ; //Royal Botanic Gardens/ ; //Natural Environment Research Council Independent Research Fellowship/ ; },
abstract = {The date palm, Phoenix dactylifera, has been a cornerstone of Middle Eastern and North African agriculture for millennia. It was first domesticated in the Persian Gulf, and its evolution appears to have been influenced by gene flow from two wild relatives, P. theophrasti, currently restricted to Crete and Turkey, and P. sylvestris, widespread from Bangladesh to the West Himalayas. Genomes of ancient date palm seeds show that gene flow from P. theophrasti to P. dactylifera may have occurred by ∼2,200 years ago, but traces of P. sylvestris could not be detected. We here integrate archeogenomics of a ∼2,100-year-old P. dactylifera leaf from Saqqara (Egypt), molecular-clock dating, and coalescence approaches with population genomic tests, to probe the hybridization between the date palm and its two closest relatives and provide minimum and maximum timestamps for its reticulated evolution. The Saqqara date palm shares a close genetic affinity with North African date palm populations, and we find clear genomic admixture from both P. theophrasti, and P. sylvestris, indicating that both had contributed to the date palm genome by 2,100 years ago. Molecular-clocks placed the divergence of P. theophrasti from P. dactylifera/P. sylvestris and that of P. dactylifera from P. sylvestris in the Upper Miocene, but strongly supported, conflicting topologies point to older gene flow between P. theophrasti and P. dactylifera, and P. sylvestris and P. dactylifera. Our work highlights the ancient hybrid origin of the date palms, and prompts the investigation of the functional significance of genetic material introgressed from both close relatives, which in turn could prove useful for modern date palm breeding.},
}

@article {pmid34002224,
year = {2021},
author = {Immel, A and Key, FM and Szolek, A and Barquera, R and Robinson, MK and Harrison, GF and Palmer, WH and Spyrou, MA and Susat, J and Krause-Kyora, B and Bos, KI and Forrest, S and Hernández-Zaragoza, DI and Sauter, J and Solloch, U and Schmidt, AH and Schuenemann, VJ and Reiter, E and Kairies, MS and Weiß, R and Arnold, S and Wahl, J and Hollenbach, JA and Kohlbacher, O and Herbig, A and Norman, PJ and Krause, J},
title = {Analysis of Genomic DNA from Medieval Plague Victims Suggests Long-Term Effect of Yersinia pestis on Human Immunity Genes.},
journal = {Molecular biology and evolution},
volume = {38},
number = {10},
pages = {4059-4076},
doi = {10.1093/molbev/msab147},
pmid = {34002224},
issn = {1537-1719},
support = {R56 AI151549/AI/NIAID NIH HHS/United States ; /ERC_/European Research Council/International ; R56 AI151549/AI/NIAID NIH HHS/United States ; },
abstract = {Pathogens and associated outbreaks of infectious disease exert selective pressure on human populations, and any changes in allele frequencies that result may be especially evident for genes involved in immunity. In this regard, the 1346-1353 Yersinia pestis-caused Black Death pandemic, with continued plague outbreaks spanning several hundred years, is one of the most devastating recorded in human history. To investigate the potential impact of Y. pestis on human immunity genes, we extracted DNA from 36 plague victims buried in a mass grave in Ellwangen, Germany in the 16th century. We targeted 488 immune-related genes, including HLA, using a novel in-solution hybridization capture approach. In comparison with 50 modern native inhabitants of Ellwangen, we find differences in allele frequencies for variants of the innate immunity proteins Ficolin-2 and NLRP14 at sites involved in determining specificity. We also observed that HLA-DRB1*13 is more than twice as frequent in the modern population, whereas HLA-B alleles encoding an isoleucine at position 80 (I-80+), HLA C*06:02 and HLA-DPB1 alleles encoding histidine at position 9 are half as frequent in the modern population. Simulations show that natural selection has likely driven these allele frequency changes. Thus, our data suggest that allele frequencies of HLA genes involved in innate and adaptive immunity responsible for extracellular and intracellular responses to pathogenic bacteria, such as Y. pestis, could have been affected by the historical epidemics that occurred in Europe.},
}

@article {pmid34547027,
year = {2021},
author = {Wu, X and Ning, C and Key, FM and Andrades Valtueña, A and Lankapalli, AK and Gao, S and Yang, X and Zhang, F and Liu, L and Nie, Z and Ma, J and Krause, J and Herbig, A and Cui, Y},
title = {A 3,000-year-old, basal S. enterica lineage from Bronze Age Xinjiang suggests spread along the Proto-Silk Road.},
journal = {PLoS pathogens},
volume = {17},
number = {9},
pages = {e1009886},
doi = {10.1371/journal.ppat.1009886},
pmid = {34547027},
issn = {1553-7374},
abstract = {Salmonella enterica (S. enterica) has infected humans for a long time, but its evolutionary history and geographic spread across Eurasia is still poorly understood. Here, we screened for pathogen DNA in 14 ancient individuals from the Bronze Age Quanergou cemetery (XBQ), Xinjiang, China. In 6 individuals we detected S. enterica. We reconstructed S. enterica genomes from those individuals, which form a previously undetected phylogenetic branch basal to Paratyphi C, Typhisuis and Choleraesuis-the so-called Para C lineage. Based on pseudogene frequency, our analysis suggests that the ancient S. enterica strains were not host adapted. One genome, however, harbors the Salmonella pathogenicity island 7 (SPI-7), which is thought to be involved in (para)typhoid disease in humans. This offers first evidence that SPI-7 was acquired prior to the emergence of human-adapted Paratyphi C around 1,000 years ago. Altogether, our results show that Salmonella enterica infected humans in Eastern Eurasia at least 3,000 years ago, and provide the first ancient DNA evidence for the spread of a pathogen along the Proto-Silk Road.},
}

@article {pmid34533260,
year = {2021},
author = {Tamburrini, C and de Saint Pierre, M and Bravi, CM and Bailliet, G and Jurado Medina, L and Velázquez, IF and Real, LE and Holley, A and Tedeschi, CM and Basso, NG and Parolin, ML},
title = {Uniparental origins of the admixed Argentine Patagonia.},
journal = {American journal of human biology : the official journal of the Human Biology Council},
volume = {},
number = {},
pages = {e23682},
doi = {10.1002/ajhb.23682},
pmid = {34533260},
issn = {1520-6300},
support = {PICT 2013-2414//Agencia Nacional de Promoción Científica y Tecnológica, Argentina/ ; JLL PICT 2014-1558//Agencia Nacional de Promoción Científica y Tecnológica, Argentina/ ; },
abstract = {OBJECTIVES: We aimed to contribute to the understanding of the ancient geographic origins of the uniparentally inherited markers in modern admixed Argentinian populations from central Patagonia with new information provided for the city of Trelew. We attempted to highlight the importance of combining different genetic markers when studying population history.

METHODS: The mtDNA control region sequence was typified in 89 individuals and 12 Y-STR and 15 Y-SNP loci were analyzed in 66 males. With these data, analysis of molecular variance and Network analyses were carried out. We exhaustively compared the modern data with ancient mtDNA information. Finally, we tested the differences in continental origins estimated by uniparental and previously published biparental markers.

RESULTS: Native American mtDNAs (53.9%) increased when maternal ancestors were born in the northern (81.8%) and southern (58.5%) regions of Argentina or in Chile (77.8%). Population substructure was only observed for Y-chromosome haplotypes. Some mtDNA haplogroups have been present in the area for at least ca. 2762-2430 and ca. 500 (D1g and D1g4 haplogroups) and ca. 6736 and ca. 6620 (C1b and C1c haplogroups) years, respectively. In contrast, haplogroups B2i2 and C1b13, frequent in modern Patagonia populations, had not been found in previous ancient DNA studies of the region.

CONCLUSIONS: The results suggest that Native American ancestry is well preserved in the region. Trelew samples had characteristic native mtDNA haplogroups previously described in Chilean and Argentine Patagonian populations, but not observed in ancient samples until now. These findings support the idea that these lineages have a recent regional origin. Finally, the estimated proportions of continental ancestry depend on the genetic marker analyzed.},
}

@article {pmid34527890,
year = {2021},
author = {de-Dios, T and Carrión, P and Olalde, I and Llovera Nadal, L and Lizano, E and Pàmies, D and Marques-Bonet, T and Balloux, F and van Dorp, L and Lalueza-Fox, C},
title = {Salmonella enterica from a soldier from the 1652 siege of Barcelona (Spain) supports historical transatlantic epidemic contacts.},
journal = {iScience},
volume = {24},
number = {9},
pages = {103021},
pmid = {34527890},
issn = {2589-0042},
abstract = {Ancient pathogen genomics is an emerging field allowing reconstruction of past epidemics. The demise of post-contact American populations may, at least in part, have been caused by paratyphoid fever brought by Europeans. We retrieved genome-wide data from two Spanish soldiers who were besieging the city of Barcelona in 1652, during the Reapers' War. Their ancestry derived from the Basque region and Sardinia, respectively, (at that time, this island belonged to the Spanish kingdom). Despite the proposed plague epidemic, we could not find solid evidence for the presence of the causative plague agent in these individuals. However, we retrieved from one individual a substantial fraction of the Salmonella enterica serovar Paratyphi C lineage linked to paratyphoid fever in colonial period Mexico. Our results support a growing body of evidence that Paratyphi C enteric fever was more prevalent in Europe and the Americas in the past than it is today.},
}

@article {pmid34521843,
year = {2021},
author = {Ringbauer, H and Novembre, J and Steinrücken, M},
title = {Parental relatedness through time revealed by runs of homozygosity in ancient DNA.},
journal = {Nature communications},
volume = {12},
number = {1},
pages = {5425},
pmid = {34521843},
issn = {2041-1723},
abstract = {Parental relatedness of present-day humans varies substantially across the globe, but little is known about the past. Here we analyze ancient DNA, leveraging that parental relatedness leaves genomic traces in the form of runs of homozygosity. We present an approach to identify such runs in low-coverage ancient DNA data aided by haplotype information from a modern phased reference panel. Simulation and experiments show that this method robustly detects runs of homozygosity longer than 4 centimorgan for ancient individuals with at least 0.3 × coverage. Analyzing genomic data from 1,785 ancient humans who lived in the last 45,000 years, we detect low rates of first cousin or closer unions across most ancient populations. Moreover, we find a marked decay in background parental relatedness co-occurring with or shortly after the advent of sedentary agriculture. We observe this signal, likely linked to increasing local population sizes, across several geographic transects worldwide.},
}

@article {pmid34518562,
year = {2021},
author = {Silva, M and Oteo-García, G and Martiniano, R and Guimarães, J and von Tersch, M and Madour, A and Shoeib, T and Fichera, A and Justeau, P and Foody, MGB and McGrath, K and Barrachina, A and Palomar, V and Dulias, K and Yau, B and Gandini, F and Clarke, DJ and Rosa, A and Brehm, A and Flaquer, A and Rito, T and Olivieri, A and Achilli, A and Torroni, A and Gómez-Carballa, A and Salas, A and Bryk, J and Ditchfield, PW and Alexander, M and Pala, M and Soares, PA and Edwards, CJ and Richards, MB},
title = {Biomolecular insights into North African-related ancestry, mobility and diet in eleventh-century Al-Andalus.},
journal = {Scientific reports},
volume = {11},
number = {1},
pages = {18121},
pmid = {34518562},
issn = {2045-2322},
support = {PTDC/SOC-ANT/30316/2017//Fundação para a Ciência e a Tecnologia/ ; PTDC/SOC-ANT/30316/2017//Fundação para a Ciência e a Tecnologia/ ; PTDC/SOC-ANT/30316/2017//Fundação para a Ciência e a Tecnologia/ ; PTDC/EPH-ARQ/4164/2014//Fundação para a Ciência e a Tecnologia/ ; PTDC/SOC-ANT/30316/2017//Fundação para a Ciência e a Tecnologia/ ; PTDC/EPH-ARQ/4164/2014//Fundação para a Ciência e a Tecnologia/ ; LMUinnovativ//Munich Center of Health Sciences (MC Health), Ludwig-Maximilians-Universität/ ; "Dipartimenti di Eccellenza" Program (2018-2022) - Department of Biology and Biotechnology "L. Spallanzani," University of Pavia and PRIN2017 20174BTC4R//Italian Ministry of Education, University and Research/ ; "Dipartimenti di Eccellenza" Program (2018-2022) - Department of Biology and Biotechnology "L. Spallanzani," University of Pavia and PRIN2017 20174BTC4R//Italian Ministry of Education, University and Research/ ; "Dipartimenti di Eccellenza" Program (2018-2022) - Department of Biology and Biotechnology "L. Spallanzani," University of Pavia and PRIN2017 20174BTC4R//Italian Ministry of Education, University and Research/ ; },
abstract = {Historical records document medieval immigration from North Africa to Iberia to create Islamic al-Andalus. Here, we present a low-coverage genome of an eleventh century CE man buried in an Islamic necropolis in Segorbe, near Valencia, Spain. Uniparental lineages indicate North African ancestry, but at the autosomal level he displays a mosaic of North African and European-like ancestries, distinct from any present-day population. Altogether, the genome-wide evidence, stable isotope results and the age of the burial indicate that his ancestry was ultimately a result of admixture between recently arrived Amazigh people (Berbers) and the population inhabiting the Peninsula prior to the Islamic conquest. We detect differences between our sample and a previously published group of contemporary individuals from Valencia, exemplifying how detailed, small-scale aDNA studies can illuminate fine-grained regional and temporal differences. His genome demonstrates how ancient DNA studies can capture portraits of past genetic variation that have been erased by later demographic shifts-in this case, most likely the seventeenth century CE expulsion of formerly Islamic communities as tolerance dissipated following the Reconquista by the Catholic kingdoms of the north.},
}

@article {pmid34516814,
year = {2021},
author = {Gibbons, A},
title = {How farming shaped Europeans' immunity.},
journal = {Science (New York, N.Y.)},
volume = {373},
number = {6560},
pages = {1186},
doi = {10.1126/science.acx9047},
pmid = {34516814},
issn = {1095-9203},
mesh = {Agriculture/*history ; Animals ; Communicable Diseases/*history/immunology/mortality ; DNA, Ancient ; Europe ; Genetic Variation ; History, Ancient ; Humans ; *Immune System ; Immunity/genetics ; Zoonoses/history/immunology ; },
abstract = {[Figure: see text].},
}

Agriculture/*history
Animals
Communicable Diseases/*history/immunology/mortality
DNA, Ancient
Europe
Genetic Variation
History, Ancient
Humans
*Immune System
Immunity/genetics
Zoonoses/history/immunology

@article {pmid34483440,
year = {2021},
author = {Sedig, JW and Olade, I and Patterson, N and Harney, É and Reich, D},
title = {COMBINING ANCIENT DNA AND RADIOCARBON DATING DATA TO INCREASE CHRONOLOGICAL ACCURACY.},
journal = {Journal of archaeological science},
volume = {133},
number = {},
pages = {},
pmid = {34483440},
issn = {0305-4403},
support = {R01 GM100233/GM/NIGMS NIH HHS/United States ; },
abstract = {This paper examines how ancient DNA data can enhance radiocarbon dating. Because there is a limit to the number of years that can separate the dates of death of related individuals, the ability to identify relatives through ancient DNA analysis can serve as a constraint on radiocarbon date range estimates. To determine the number of years that can separate related individuals, we modeled maximums derived from biological extremes of human reproduction and death ages and compiled data from historic and genealogical death records. We used these data to jointly study the date ranges of a global dataset of individuals that have been radiocarbon dated and for which ancient DNA analysis identified at least one relative. We found that many of these individuals could have their date uncertainties reduced by building in date of death separation constraints. We examined possible reasons for date discrepancies of related individuals, such as dating of different skeletal elements or wiggles in the radiocarbon curve. We also developed a program, refinedate, which researchers can download and use to help refine the radiocarbon date distributions of related individuals. Our research demonstrates that when combined, radiocarbon dating and ancient DNA analysis can provide a refined and richer view of the past.},
}

@article {pmid34475454,
year = {2021},
author = {Kehlmaier, C and López-Jurado, LF and Hernández-Acosta, N and Mateo-Miras, A and Fritz, U},
title = {"Ancient DNA" reveals that the scientific name for an extinct tortoise from Cape Verde refers to an extant South American species.},
journal = {Scientific reports},
volume = {11},
number = {1},
pages = {17537},
pmid = {34475454},
issn = {2045-2322},
abstract = {We examined the type material of the extinct tortoise species Geochelone atlantica López-Jurado, Mateo and García-Márquez, 1998 from Sal Island, Cape Verde, using aDNA approaches and AMS radiocarbon dating. High-quality mitochondrial genomes obtained from the three type specimens support that all type material belongs to the same individual. In phylogenetic analyses using mitochondrial genomes of all species groups and genera of extant and some recently extinct tortoises, the type material clusters within the extant South American red-footed tortoise Chelonoidis carbonarius (Spix, 1824). Our radiocarbon date indicates that the tortoise from which the type series of G. atlantica originates was still alive during 1962 and 1974. These results provide firm evidence that the type material of G. atlantica does not belong to the Quaternary tortoise bones excavated on Sal Island in the 1930s, as originally thought. Thus, the extinct tortoise species remains unstudied and lacks a scientific name, and the name G. atlantica has to be relegated into the synonymy of C. carbonarius. The circumstances that led to this confusion currently cannot be disentangled.},
}

@article {pmid34465619,
year = {2021},
author = {Bramanti, B and Wu, Y and Yang, R and Cui, Y and Stenseth, NC},
title = {Assessing the origins of the European Plagues following the Black Death: A synthesis of genomic, historical, and ecological information.},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {118},
number = {36},
pages = {},
doi = {10.1073/pnas.2101940118},
pmid = {34465619},
issn = {1091-6490},
abstract = {The second plague pandemic started in Europe with the Black Death in 1346 and lasted until the 19th century. Based on ancient DNA studies, there is a scientific disagreement over whether the bacterium, Yersinia pestis, came into Europe once (Hypothesis 1) or repeatedly over the following four centuries (Hypothesis 2). Here, we synthesize the most updated phylogeny together with historical, archeological, evolutionary, and ecological information. On the basis of this holistic view, we conclude that Hypothesis 2 is the most plausible. We also suggest that Y. pestis lineages might have developed attenuated virulence during transmission, which can explain the convergent evolutionary signals, including pla decay, that appeared at the end of the pandemics.},
}

@article {pmid34459346,
year = {2021},
author = {Caramelli, D and Posth, C and Rickards, O},
title = {Reconstruction of the human peopling of Europe: a genetic insight.},
journal = {Annals of human biology},
volume = {48},
number = {3},
pages = {175-178},
doi = {10.1080/03014460.2021.1955472},
pmid = {34459346},
issn = {1464-5033},
}

@article {pmid34459345,
year = {2021},
author = {Feldman, M and Gnecchi-Ruscone, GA and Lamnidis, TC and Posth, C},
title = {Where Asia meets Europe - recent insights from ancient human genomics.},
journal = {Annals of human biology},
volume = {48},
number = {3},
pages = {191-202},
doi = {10.1080/03014460.2021.1949039},
pmid = {34459345},
issn = {1464-5033},
abstract = {CONTEXT: The peopling of Europe by modern humans is a widely debated topic in the field of modern and ancient genomics. While several recent syntheses have focussed on this topic, little has been discussed about the genetic history of populations in the continent's surrounding regions.

OBJECTIVE: We explore genetic transformations in three key areas that played an essential role in the formation of the European genetic landscape through time, focussing on the periods spanning from the Epipalaeolithic/Mesolithic and up until the Iron Age.

METHODS: We review published ancient genomic studies and integrate the associated data to provide a quantification and visualisation of major trends in the population histories of the Near East, the western Eurasian Steppe and North East Europe.

RESULTS: We describe cross-regional as well as localised prehistoric demographic shifts and discuss potential research directions while highlighting geo-temporal gaps in the data.

CONCLUSION: In recent years, archaeogenetic studies have contributed to the understanding of human genetic diversity through time in regions located at the doorstep of Europe. Further studies focussing on these areas will allow for a better characterisation of genetic shifts and regionally-specific patterns of admixture across western Eurasia.},
}

@article {pmid34459344,
year = {2021},
author = {Modi, A and Vai, S and Posth, C and Vergata, C and Zaro, V and Diroma, MA and Boschin, F and Capecchi, G and Ricci, S and Ronchitelli, A and Catalano, G and Lauria, G and D'Amore, G and Sineo, L and Caramelli, D and Lari, M},
title = {More data on ancient human mitogenome variability in Italy: new mitochondrial genome sequences from three Upper Palaeolithic burials.},
journal = {Annals of human biology},
volume = {48},
number = {3},
pages = {213-222},
doi = {10.1080/03014460.2021.1942549},
pmid = {34459344},
issn = {1464-5033},
abstract = {BACKGROUND: Recently, the study of mitochondrial variability in ancient humans has allowed the definition of population dynamics that characterised Europe in the Late Pleistocene and Early Holocene. Despite the abundance of sites and skeletal remains few data are available for Italy.

AIM: We reconstructed the mitochondrial genomes of three Upper Palaeolithic individuals for some of the most important Italian archaeological contexts: Paglicci (South-Eastern Italy), San Teodoro (South-Western Italy) and Arene Candide (North-Western Italy) caves.

SUBJECTS AND METHODS: We explored the phylogenetic relationships of the three mitogenomes in the context of Western Eurasian ancient and modern variability.

RESULTS: Paglicci 12 belongs to sub-haplogroup U8c, described in only two other Gravettian individuals; San Teodoro 2 harbours a U2'3'4'7'8'9 sequence, the only lineage found in Sicily during the Late Pleistocene and Early Holocene; Arene Candide 16 displays an ancestral U5b1 haplotype already detected in other Late Pleistocene hunter-gatherers from Central Europe.

CONCLUSION: Regional genetic continuity is highlighted in the Gravettian groups that succeeded in Paglicci. Data from one of the oldest human remains from Sicily reinforce the hypothesis that Epigravettian groups carrying U2'3'4'7'8'9 could be the first inhabitants of the island. The first pre-Neolithic mitogenome from North-Western Italy, sequenced here, shows more affinity with continental Europe than with the Italian peninsula.},
}

@article {pmid34459342,
year = {2021},
author = {Serrano, JG and Ordóñez, AC and Fregel, R},
title = {Paleogenomics of the prehistory of Europe: human migrations, domestication and disease.},
journal = {Annals of human biology},
volume = {48},
number = {3},
pages = {179-190},
doi = {10.1080/03014460.2021.1942205},
pmid = {34459342},
issn = {1464-5033},
abstract = {A substantial portion of ancient DNA research has been centred on understanding European populations' origin and evolution. A rchaeological evidence has already shown that the peopling of Europe involved an intricate pattern of demic and/or cultural diffusion since the Upper Palaeolithic, which became more evident during the Neolithic and Bronze Age periods. However, ancient DNA data has been crucial in determining if cultural changes occurred due to the movement of ideas or people. With the advent of next-generation sequencing and population-based paleogenomic research, ancient DNA studies have been directed not only at the study of continental human migrations, but also to the detailed analysis of particular archaeological sites, the processes of domestication, or the spread of disease during prehistoric times. With this vast paleogenomic effort added to a proper archaeological contextualisation of results, a deeper understanding of Europe's peopling is starting to emanate.},
}

@article {pmid34459340,
year = {2021},
author = {Sarno, S and Cilli, E and Serventi, P and De Fanti, S and Corona, A and Fontani, F and Traversari, M and Ferri, G and Fariselli, AC and Luiselli, D},
title = {Insights into Punic genetic signatures in the southern necropolis of Tharros (Sardinia).},
journal = {Annals of human biology},
volume = {48},
number = {3},
pages = {247-259},
doi = {10.1080/03014460.2021.1937699},
pmid = {34459340},
issn = {1464-5033},
abstract = {BACKGROUND: Phoenician and Punic expansions have been protagonists of intense trade networks and settlements in the Mediterranean Sea.

AIMS: The maternal genetic variability of ancient Punic samples from the Sardinian necropolis of Tharros was analysed, with the aim to explore genetic interactions and signatures of past population events.

SUBJECTS AND METHODS: The mtDNA HVS-I and coding region SNPs were analysed in 14 Punic samples and 74 modern individuals from Cabras and Belvì (for which the HVS-II region was also analysed). The results were compared with 5,590 modern Euro-Mediterranean sequences and 127 ancient samples.

RESULTS: While contemporary groups fall within the genetic variability of other modern Sardinians, our Punic samples reveal proximity to present-day North-African and Iberian populations. Furthermore, Cabras and Belvì cluster mainly with pre-Phoenician groups, while samples from Tharros project with other Punic Sardinian individuals.

CONCLUSION: This study provides the first preliminary insights into the population dynamics of the Punic site of Tharros. While the number of currently available samples does not allow definitive investigation of the connection with indigenous Sardinian groups, our results seem to confirm internal migratory phenomena in the central-western Mediterranean and female participation in the Punic mobility.},
}

@article {pmid34459338,
year = {2021},
author = {De Angelis, F and Veltre, V and Romboni, M and Di Corcia, T and Scano, G and Martínez-Labarga, C and Catalano, P and Rickards, O},
title = {Ancient genomes from a rural site in Imperial Rome (1st-3rd cent. CE): a genetic junction in the Roman Empire.},
journal = {Annals of human biology},
volume = {48},
number = {3},
pages = {234-246},
doi = {10.1080/03014460.2021.1944313},
pmid = {34459338},
issn = {1464-5033},
abstract = {BACKGROUND: Rome became the prosperous Capital of the Roman Empire through the political and military conquests of neighbouring areas. People were able to move Romeward modifying the Rome area's demographic structure. However, the genomic evidence for the population of one of the broadest Empires in antiquity has been sparse until recently.

AIM: The genomic analysis of people buried in Quarto Cappello del Prete (QCP) necropolis was carried out to help elucidate the genomic structure of Imperial Rome inhabitants.

SUBJECTS AND METHODS: We recruited twenty-five individuals from QCP for ancient DNA analysis through whole-genome sequencing. Multiple investigations were carried out to unveil the genetic components featuring in the studied samples and the community's putative demographic structure.

RESULTS: We generated reliable whole-genome data for 7 samples surviving quality controls. The distribution of Imperial Romans from QCP partly overlaps with present-day Southern Mediterranean and Southern-Near Eastern populations.

CONCLUSION: The genomic legacy with the south-eastern shores of the Mediterranean Sea and the Central and Western Northern-African coast funerary influence pave the way for considering people buried in QCP as resembling a Punic-derived human group.},
}

@article {pmid34456378,
year = {2020},
author = {Forin, N and Vizzini, A and Nigris, S and Ercole, E and Voyron, S and Girlanda, M and Baldan, B},
title = {Illuminating type collections of nectriaceous fungi in Saccardo's fungarium.},
journal = {Persoonia},
volume = {45},
number = {},
pages = {221-249},
pmid = {34456378},
issn = {0031-5850},
abstract = {Specimens of Nectria spp. and Nectriella rufofusca were obtained from the fungarium of Pier Andrea Saccardo, and investigated via a morphological and molecular approach based on MiSeq technology. ITS1 and ITS2 sequences were successfully obtained from 24 specimens identified as 'Nectria' sensu Saccardo (including 20 types) and from the type specimen of Nectriella rufofusca. For Nectria ambigua, N. radians and N. tjibodensis only the ITS1 sequence was recovered. On the basis of morphological and molecular analyses new nomenclatural combinations for Nectria albofimbriata, N. ambigua, N. ambigua var. pallens, N. granuligera, N. peziza subsp. reyesiana, N. radians, N. squamuligera, N. tjibodensis and new synonymies for N. congesta, N. flageoletiana, N. phyllostachydis, N. sordescens and N. tjibodensis var. crebrior are proposed. Furthermore, the current classification is confirmed for Nectria coronata, N. cyanostoma, N. dolichospora, N. illudens, N. leucotricha, N. mantuana, N. raripila and Nectriella rufofusca. This is the first time that these more than 100-yr-old specimens are subjected to molecular analysis, thereby providing important new DNA sequence data authentic for these names.},
}

@article {pmid34456066,
year = {2021},
author = {Raxworthy, CJ and Smith, BT},
title = {Mining museums for historical DNA: advances and challenges in museomics.},
journal = {Trends in ecology & evolution},
volume = {},
number = {},
pages = {},
doi = {10.1016/j.tree.2021.07.009},
pmid = {34456066},
issn = {1872-8383},
abstract = {Historical DNA (hDNA), obtained from museum and herbarium specimens, has yielded spectacular new insights into the history of organisms. This includes documenting historical genetic erosion and extinction, discovering species new to science, resolving evolutionary relationships, investigating epigenetic effects, and determining origins of infectious diseases. However, the development of best-practices in isolating, processing, and analyzing hDNA remain under-explored, due to the substantial diversity of specimen preparation types, tissue sources, archival ages, and collecting histories. Thus, for hDNA to reach its full potential, and justify the destructive sampling of the rarest specimens, more experimental work using time-series collections, and the development of improved methods to correct for data asymmetries and biases due to DNA degradation are required.},
}

@article {pmid34455262,
year = {2021},
author = {Buck, LT and Katz, DC and Ackermann, RR and Hlusko, LJ and Kanthaswamy, S and Weaver, TD},
title = {Effects of hybridization on pelvic morphology: A macaque model.},
journal = {Journal of human evolution},
volume = {159},
number = {},
pages = {103049},
doi = {10.1016/j.jhevol.2021.103049},
pmid = {34455262},
issn = {1095-8606},
abstract = {Ancient DNA analyses have shown that interbreeding between hominin taxa occurred multiple times. Although admixture is often reflected in skeletal phenotype, the relationship between the two remains poorly understood, hampering interpretation of the hominin fossil record. Direct study of this relationship is often impossible due to the paucity of hominin fossils and difficulties retrieving ancient genetic material. Here, we use a sample of known ancestry hybrids between two closely related nonhuman primate taxa (Indian and Chinese Macaca mulatta) to investigate the effect of admixture on skeletal morphology. We focus on pelvic shape, which has potential fitness implications in hybrids, as mismatches between maternal pelvic and fetal cranial morphology are often fatal to mother and offspring. As the pelvis is also one of the skeletal regions that differs most between Homo sapiens and Neanderthals, investigating the pelvic consequences of interbreeding could be informative regarding the viability of their hybrids. We find that the effect of admixture in M. mulatta is small and proportional to the relatively small morphological difference between the parent taxa. Sexual dimorphism appears to be the main determinant of pelvic shape in M. mulatta. The lack of difference in pelvic shape between Chinese and Indian M. mulatta is in contrast to that between Neanderthals and H. sapiens, despite a similar split time (in generations) between the hybridizing pairs. Greater phenotypic divergence between hominins may relate to adaptations to disparate environments but may also highlight how the unique degree of cultural buffering in hominins allowed for greater neutral divergence. In contrast to some previous work identifying extreme morphologies in first- and second-generation hybrids, here the relationship between pelvic shape and admixture is linear. This linearity may be because most sampled animals have a multigenerational admixture history or because of relatively high constraints on the pelvis compared with other skeletal regions.},
}

@article {pmid34454138,
year = {2021},
author = {Thomas, ZA and Mooney, S and Cadd, H and Baker, A and Turney, C and Schneider, L and Hogg, A and Haberle, S and Green, K and Weyrich, LS and Pérez, V and Moore, NE and Zawadzki, A and Kelloway, SJ and Khan, SJ},
title = {Late Holocene climate anomaly concurrent with fire activity and ecosystem shifts in the eastern Australian Highlands.},
journal = {The Science of the total environment},
volume = {802},
number = {},
pages = {149542},
doi = {10.1016/j.scitotenv.2021.149542},
pmid = {34454138},
issn = {1879-1026},
abstract = {The alpine area of the Australian mainland is highly sensitive to climate and environmental change, and potentially vulnerable to ecosystem tipping points. Over the next two decades the Australian alpine region is predicted to experience temperature increases of at least 1 °C, coupled with a substantial decrease in snow cover. Extending the short instrumental record in these regions is imperative to put future change into context, and potentially provide analogues of warming. We reconstructed past temperatures, using a lipid biomarker palaeothermometer technique and mercury flux changes for the past 3500 years from the sediments of Club Lake, a high-altitude alpine tarn in the Snowy Mountains, southeastern Australia. Using a multi-proxy framework, including pollen and charcoal analyses, high-resolution geochemistry, and ancient microbial community composition, supported by high-resolution 210Pb and AMS 14C dating, we investigated local and regional ecological and environmental changes occurring in response to changes in temperature. We find the region experienced a general warming trend over the last 3500 years, with a pronounced climate anomaly occurring between 1000 and 1600 cal yrs. BP. Shifts in vegetation took place during this warm period, characterised by a decline in alpine species and an increase in open woodland taxa which co-occurred with an increase in regional fire activity. Given the narrow altitudinal band of Australian alpine vegetation, any future warming has the potential to result in the extinction of alpine species, including several endemic to the area, as treelines are driven to higher elevations. These findings suggest ongoing conservation efforts will be needed to protect the vulnerable alpine environments from the combined threats of climate changes, fire and invasive species.},
}

@article {pmid34442857,
year = {2021},
author = {Nwosu, EC and Roeser, P and Yang, S and Ganzert, L and Dellwig, O and Pinkerneil, S and Brauer, A and Dittmann, E and Wagner, D and Liebner, S},
title = {From Water into Sediment-Tracing Freshwater Cyanobacteria via DNA Analyses.},
journal = {Microorganisms},
volume = {9},
number = {8},
pages = {},
pmid = {34442857},
issn = {2076-2607},
support = {BR2208/11-1//Deutsche Forschungsgemeinschaft/ ; VH-NG-919//Helmholtz-Gemeinschaft/ ; SAW-2017-IOW-2 649//Leibniz-Gemeinschaft/ ; Terrestrial Environmental Observatory Infrastructure Initiative (TERENO Observatory NE Germany//Helmholtz Association/ ; },
abstract = {Sedimentary ancient DNA-based studies have been used to probe centuries of climate and environmental changes and how they affected cyanobacterial assemblages in temperate lakes. Due to cyanobacteria containing potential bloom-forming and toxin-producing taxa, their approximate reconstruction from sediments is crucial, especially in lakes lacking long-term monitoring data. To extend the resolution of sediment record interpretation, we used high-throughput sequencing, amplicon sequence variant (ASV) analysis, and quantitative PCR to compare pelagic cyanobacterial composition to that in sediment traps (collected monthly) and surface sediments in Lake Tiefer See. Cyanobacterial composition, species richness, and evenness was not significantly different among the pelagic depths, sediment traps and surface sediments (p > 0.05), indicating that the cyanobacteria in the sediments reflected the cyanobacterial assemblage in the water column. However, total cyanobacterial abundances (qPCR) decreased from the metalimnion down the water column. The aggregate-forming (Aphanizomenon) and colony-forming taxa (Snowella) showed pronounced sedimentation. In contrast, Planktothrix was only very poorly represented in sediment traps (meta- and hypolimnion) and surface sediments, despite its highest relative abundance at the thermocline (10 m water depth) during periods of lake stratification (May-October). We conclude that this skewed representation in taxonomic abundances reflects taphonomic processes, which should be considered in future DNA-based paleolimnological investigations.},
}

@article {pmid34437844,
year = {2021},
author = {Brealey, JC and Leitão, HG and Hofstede, T and Kalthoff, DC and Guschanski, K},
title = {The oral microbiota of wild bears in Sweden reflects the history of antibiotic use by humans.},
journal = {Current biology : CB},
volume = {},
number = {},
pages = {},
doi = {10.1016/j.cub.2021.08.010},
pmid = {34437844},
issn = {1879-0445},
abstract = {Following the advent of industrial-scale antibiotic production in the 1940s,1 antimicrobial resistance (AMR) has been on the rise and now poses a major global health threat in terms of mortality, morbidity, and economic burden.2,3 Because AMR can be exchanged between humans, livestock, and wildlife, wild animals can be used as indicators of human-associated AMR contamination of the environment.4 However, AMR is a normal function of natural environments and is present in host-associated microbiomes, which makes it challenging to distinguish between anthropogenic and natural sources.4,5 One way to overcome this difficulty is to use historical samples that span the period from before the mass production of antibiotics to today. We used shotgun metagenomic sequencing of dental calculus, the calcified form of the oral microbial biofilm, to determine the abundance and repertoire of AMR genes in the oral microbiome of Swedish brown bears collected over the last 180 years. Our temporal metagenomics approach allowed us to establish a baseline of natural AMR in the pre-antibiotics era and to quantify a significant increase in total AMR load and diversity of AMR genes that is consistent with patterns of national human antibiotic use. We also demonstrated a significant decrease in total AMR load in bears in the last two decades, which coincides with Swedish strategies to mitigate AMR. Our study suggests that public health policies can be effective in limiting human-associated AMR contamination of the environment and wildlife.},
}

@article {pmid34433011,
year = {2021},
author = {Liu, S and Westbury, MV and Dussex, N and Mitchell, KJ and Sinding, MS and Heintzman, PD and Duchêne, DA and Kapp, JD and von Seth, J and Heiniger, H and Sánchez-Barreiro, F and Margaryan, A and André-Olsen, R and De Cahsan, B and Meng, G and Yang, C and Chen, L and van der Valk, T and Moodley, Y and Rookmaaker, K and Bruford, MW and Ryder, O and Steiner, C and Bruins-van Sonsbeek, LGR and Vartanyan, S and Guo, C and Cooper, A and Kosintsev, P and Kirillova, I and Lister, AM and Marques-Bonet, T and Gopalakrishnan, S and Dunn, RR and Lorenzen, ED and Shapiro, B and Zhang, G and Antoine, PO and Dalén, L and Gilbert, MTP},
title = {Ancient and modern genomes unravel the evolutionary history of the rhinoceros family.},
journal = {Cell},
volume = {184},
number = {19},
pages = {4874-4885.e16},
doi = {10.1016/j.cell.2021.07.032},
pmid = {34433011},
issn = {1097-4172},
abstract = {Only five species of the once-diverse Rhinocerotidae remain, making the reconstruction of their evolutionary history a challenge to biologists since Darwin. We sequenced genomes from five rhinoceros species (three extinct and two living), which we compared to existing data from the remaining three living species and a range of outgroups. We identify an early divergence between extant African and Eurasian lineages, resolving a key debate regarding the phylogeny of extant rhinoceroses. This early Miocene (∼16 million years ago [mya]) split post-dates the land bridge formation between the Afro-Arabian and Eurasian landmasses. Our analyses also show that while rhinoceros genomes in general exhibit low levels of genome-wide diversity, heterozygosity is lowest and inbreeding is highest in the modern species. These results suggest that while low genetic diversity is a long-term feature of the family, it has been particularly exacerbated recently, likely reflecting recent anthropogenic-driven population declines.},
}

@article {pmid34429408,
year = {2021},
author = {Beyer, RM and Krapp, M and Eriksson, A and Manica, A},
title = {Climatic windows for human migration out of Africa in the past 300,000 years.},
journal = {Nature communications},
volume = {12},
number = {1},
pages = {4889},
pmid = {34429408},
issn = {2041-1723},
mesh = {Africa ; Animals ; Biological Evolution ; *DNA, Ancient ; Emigration and Immigration ; Fossils ; Genetic Variation ; Genome, Human ; History, Ancient ; Hominidae/*genetics ; Human Migration/*history ; Humans ; Models, Genetic ; },
abstract = {Whilst an African origin of modern humans is well established, the timings and routes of their expansions into Eurasia are the subject of heated debate, due to the scarcity of fossils and the lack of suitably old ancient DNA. Here, we use high-resolution palaeoclimate reconstructions to estimate how difficult it would have been for humans in terms of rainfall availability to leave the African continent in the past 300k years. We then combine these results with an anthropologically and ecologically motivated estimate of the minimum level of rainfall required by hunter-gatherers to survive, allowing us to reconstruct when, and along which geographic paths, expansions out of Africa would have been climatically feasible. The estimated timings and routes of potential contact with Eurasia are compatible with archaeological and genetic evidence of human expansions out of Africa, highlighting the key role of palaeoclimate variability for modern human dispersals.},
}

Africa
Animals
Biological Evolution
*DNA, Ancient
Emigration and Immigration
Fossils
Genetic Variation
Genome, Human
History, Ancient
Hominidae/*genetics
Human Migration/*history
Humans
Models, Genetic

@article {pmid34428961,
year = {2021},
author = {Dussex, N and Bergfeldt, N and de Anca Prado, V and Dehasque, M and Díez-Del-Molino, D and Ersmark, E and Kanellidou, F and Larsson, P and Lemež, Š and Lord, E and Mármol-Sánchez, E and Meleg, IN and Måsviken, J and Naidoo, T and Studerus, J and Vicente, M and von Seth, J and Götherström, A and Dalén, L and Heintzman, PD},
title = {Integrating multi-taxon palaeogenomes and sedimentary ancient DNA to study past ecosystem dynamics.},
journal = {Proceedings. Biological sciences},
volume = {288},
number = {1957},
pages = {20211252},
pmid = {34428961},
issn = {1471-2954},
abstract = {Ancient DNA (aDNA) has played a major role in our understanding of the past. Important advances in the sequencing and analysis of aDNA from a range of organisms have enabled a detailed understanding of processes such as past demography, introgression, domestication, adaptation and speciation. However, to date and with the notable exception of microbiomes and sediments, most aDNA studies have focused on single taxa or taxonomic groups, making the study of changes at the community level challenging. This is rather surprising because current sequencing and analytical approaches allow us to obtain and analyse aDNA from multiple source materials. When combined, these data can enable the simultaneous study of multiple taxa through space and time, and could thus provide a more comprehensive understanding of ecosystem-wide changes. It is therefore timely to develop an integrative approach to aDNA studies by combining data from multiple taxa and substrates. In this review, we discuss the various applications, associated challenges and future prospects of such an approach.},
}

@article {pmid34428419,
year = {2021},
author = {Linderholm, A},
title = {Palaeogenetics: Dirt, what is it good for? Everything.},
journal = {Current biology : CB},
volume = {31},
number = {16},
pages = {R993-R995},
doi = {10.1016/j.cub.2021.06.074},
pmid = {34428419},
issn = {1879-0445},
abstract = {Researchers in the ancient DNA community have suspected for well over a decade that ancient whole genomes can be found in sediments. Three new studies now provide such evidence and, with it, endless possibilities for future studies of sedimentary ancient DNA.},
}

@article {pmid34424926,
year = {2021},
author = {Zacho, CM and Bager, MA and Margaryan, A and Gravlund, P and Galatius, A and Rasmussen, AR and Allentoft, ME},
title = {Uncovering the genomic and metagenomic research potential in old ethanol-preserved snakes.},
journal = {PloS one},
volume = {16},
number = {8},
pages = {e0256353},
pmid = {34424926},
issn = {1932-6203},
abstract = {Natural history museum collections worldwide represent a tremendous resource of information on past and present biodiversity. Fish, reptiles, amphibians and many invertebrate collections have often been preserved in ethanol for decades or centuries and our knowledge on the genomic and metagenomic research potential of such material is limited. Here, we use ancient DNA protocols, combined with shotgun sequencing to test the molecular preservation in liver, skin and bone tissue from five old (1842 to 1964) museum specimens of the common garter snake (Thamnophis sirtalis). When mapping reads to a T. sirtalis reference genome, we find that the DNA molecules are highly damaged with short average sequence lengths (38-64 bp) and high C-T deamination, ranging from 9% to 21% at the first position. Despite this, the samples displayed relatively high endogenous DNA content, ranging from 26% to 56%, revealing that genome-scale analyses are indeed possible from all specimens and tissues included here. Of the three tested types of tissue, bone shows marginally but significantly higher DNA quality in these metrics. Though at least one of the snakes had been exposed to formalin, neither the concentration nor the quality of the obtained DNA was affected. Lastly, we demonstrate that these specimens display a diverse and tissue-specific microbial genetic profile, thus offering authentic metagenomic data despite being submerged in ethanol for many years. Our results emphasize that historical museum collections continue to offer an invaluable source of information in the era of genomics.},
}

@article {pmid34424406,
year = {2021},
author = {Kumar, L and Farias, K and Prakash, S and Mishra, A and Mustak, MS and Rai, N and Thangaraj, K},
title = {Dissecting the genetic history of the Roman Catholic populations of West Coast India.},
journal = {Human genetics},
volume = {140},
number = {10},
pages = {1487-1498},
pmid = {34424406},
issn = {1432-1203},
abstract = {Migration and admixture history of populations have always been curious and an interesting theme. The West Coast of India harbours a rich diversity, bestowing various ethno-linguistic groups, with many of them having well-documented history of migrations. The Roman Catholic is one such distinct group, whose origin was much debated. While some historians and anthropologists relating them to ancient group of Gaud Saraswat Brahmins, others relating them for being members of the Jews Lost Tribes in the first Century migration to India. Historical records suggests that this community was later forcibly converted to Christianity by the Portuguese in Goa during the Sixteenth Century. Till date, no genetic study was done on this group to infer their origin and genetic affinity. Hence, we analysed 110 Roman Catholics from three different locations of West Coast of India including Goa, Kumta and Mangalore using both uniparental and autosomal markers to understand their genetic history. We found that the Roman Catholics have close affinity with the Indo-European linguistic groups, particularly Brahmins. Additionally, we detected genetic signal of Jews in the linkage disequilibrium-based admixture analysis, which was absent in other Indo-European populations, who are inhabited in the same geographical regions. Haplotype-based analysis suggests that the Roman Catholics consist of South Asian-specific ancestry and showed high drift. Ancestry-specific historical population size estimation points to a possible bottleneck around the time of Goan inquisition (fifteenth century). Analysis of the Roman Catholics data along with ancient DNA data of Neolithic and bronze age revealed that the Roman Catholics fits well in a basic model of ancient ancestral composition, typical of most of the Indo-European caste groups of India. Mitochondrial DNA (mtDNA) analysis suggests that most of the Roman Catholics have aboriginal Indian maternal genetic ancestry; while the Y chromosomal DNA analysis indicates high frequency of R1a lineage, which is predominant in groups with higher ancestral North Indian (ANI) component. Therefore, we conclude that the Roman Catholics of Goa, Kumta and Mangalore regions are the remnants of very early lineages of Brahmin community of India, having Indo-Europeans genetic affinity along with cryptic Jewish admixture, which needs to be explored further.},
}

@article {pmid34422004,
year = {2021},
author = {Irving-Pease, EK and Muktupavela, R and Dannemann, M and Racimo, F},
title = {Quantitative Human Paleogenetics: What can Ancient DNA Tell us About Complex Trait Evolution?.},
journal = {Frontiers in genetics},
volume = {12},
number = {},
pages = {703541},
pmid = {34422004},
issn = {1664-8021},
abstract = {Genetic association data from national biobanks and large-scale association studies have provided new prospects for understanding the genetic evolution of complex traits and diseases in humans. In turn, genomes from ancient human archaeological remains are now easier than ever to obtain, and provide a direct window into changes in frequencies of trait-associated alleles in the past. This has generated a new wave of studies aiming to analyse the genetic component of traits in historic and prehistoric times using ancient DNA, and to determine whether any such traits were subject to natural selection. In humans, however, issues about the portability and robustness of complex trait inference across different populations are particularly concerning when predictions are extended to individuals that died thousands of years ago, and for which little, if any, phenotypic validation is possible. In this review, we discuss the advantages of incorporating ancient genomes into studies of trait-associated variants, the need for models that can better accommodate ancient genomes into quantitative genetic frameworks, and the existing limits to inferences about complex trait evolution, particularly with respect to past populations.},
}

@article {pmid34421956,
year = {2021},
author = {Li, W and Ma, Q and Yin, P and Wen, J and Pei, Y and Niu, L and Lin, H},
title = {The GA 20-Oxidase Encoding Gene MSD1 Controls the Main Stem Elongation in Medicago truncatula.},
journal = {Frontiers in plant science},
volume = {12},
number = {},
pages = {709625},
pmid = {34421956},
issn = {1664-462X},
abstract = {Plant height is an important agronomic trait that is closely related to biomass yield and crop production. Despite legumes comprise one of the largest monophyletic families that are second only to grasses in terms of economic and nutritional values, due to an ancient genome duplication event, most legume plants have complex genomes, thus the molecular mechanisms that determine plant height are less known in legumes. Here, we report the identification and characterization of MAIN STEM DWARF1 (MSD1), which is required for the plant height in the model legume Medicago truncatula. Loss of function of MSD1 leads to severely reduced main stem height but normal lateral branch elongation in M. truncatula. Histological analysis revealed that the msd1-1 main stem has shorter internodes with reduced cell size and number compared with the wild type, indicating that MSD1 affects cell elongation and cell proliferation. MSD1 encodes a putative GA 20-oxidase that is expressed at significantly higher levels in the main shoot apex than in the lateral shoot apices, suggesting that MSD1 expression is associated with its effect on the main stem elongation. UPLC-MS/MS analysis showed that GA9 and GA4, two identified products of the GA 20-oxidase, were severely reduced in msd1-1, and the dwarf phenotype of msd1-1 could be rescued by supplementation with gibberellic acid GA3, confirming that MSD1 functions as a biologically active GA 20-oxidase. Moreover, we found that disruption of either MtGA20ox7 or MtGA20ox8, homologs of MSD1, has little effects on the elongation of the main stem, while the msd1-1 mtga20ox7-1 mtga20ox8 triple mutants exhibits a severe short main shoot and lateral branches, as well as reduced leaf size, suggesting that MSD1 and its homologs MtGA20ox7 and MtGA20ox8, redundantly regulate M. truncatula shoot elongation and leaf development. Taken together, our findings demonstrate the molecular mechanism of MSD1-mediated regulation of main stem elongation in M. truncatula and provide insights into understanding the functional diversity of GA 20-oxidases in optimizing plant architecture in legumes.},
}

@article {pmid34419425,
year = {2021},
author = {Liu, Y and Wang, T and Wu, X and Fan, X and Wang, W and Xie, G and Li, Z and Yang, Q and Cao, P and Yang, R and Liu, F and Dai, Q and Feng, X and Ping, W and Miao, B and Wu, Y and Liu, Y and Fu, Q},
title = {Maternal genetic history of southern East Asians over the past 12,000 years.},
journal = {Journal of genetics and genomics = Yi chuan xue bao},
volume = {},
number = {},
pages = {},
doi = {10.1016/j.jgg.2021.06.002},
pmid = {34419425},
issn = {1673-8527},
abstract = {Southern East Asia, including Guangxi and Fujian provinces in China, is home to diverse ethnic groups, languages, and cultures. Previous studies suggest a high complexity regarding population dynamics and the history of southern East Asians. However, large-scale genetic studies on ancient populations in this region are hindered by limited sample preservation. Here, using highly efficient DNA capture techniques, we obtain 48 complete mitochondrial genomes of individuals from Guangxi and Fujian in China and reconstruct their maternal genetic history over the past 12,000 years. We find a strong connection between southern East Asians dating to ~12,000-6000 years ago and present-day Southeast Asians. In addition, stronger genetic affinities to northern East Asians are observed in historical southern East Asians than Neolithic southern East Asians, suggesting increased interactions between northern and southern East Asians over time. Overall, we reveal dynamic connections between ancient southern East Asians and populations located in surrounding regions, as well as a shift in maternal genetic structure within the populations over time.},
}

@article {pmid34411538,
year = {2021},
author = {Kivisild, T and Saag, L and Hui, R and Biagini, SA and Pankratov, V and D'Atanasio, E and Pagani, L and Saag, L and Rootsi, S and Mägi, R and Metspalu, E and Valk, H and Malve, M and Irdt, K and Reisberg, T and Solnik, A and Scheib, CL and Seidman, DN and Williams, AL and , and Tambets, K and Metspalu, M},
title = {Patterns of genetic connectedness between modern and medieval Estonian genomes reveal the origins of a major ancestry component of the Finnish population.},
journal = {American journal of human genetics},
volume = {108},
number = {9},
pages = {1792-1806},
doi = {10.1016/j.ajhg.2021.07.012},
pmid = {34411538},
issn = {1537-6605},
support = {2000368/Z/15/Z/WT_/Wellcome Trust/United Kingdom ; R35 GM133805/GM/NIGMS NIH HHS/United States ; },
mesh = {*Alleles ; DNA, Ancient/*analysis ; Estonia ; Female ; Finland ; Gene Frequency ; Genealogy and Heraldry ; *Genome, Human ; High-Throughput Nucleotide Sequencing ; History, 21st Century ; History, Ancient ; History, Medieval ; Human Migration/*history ; Humans ; Language/history ; Male ; *Pedigree ; },
abstract = {The Finnish population is a unique example of a genetic isolate affected by a recent founder event. Previous studies have suggested that the ancestors of Finnic-speaking Finns and Estonians reached the circum-Baltic region by the 1st millennium BC. However, high linguistic similarity points to a more recent split of their languages. To study genetic connectedness between Finns and Estonians directly, we first assessed the efficacy of imputation of low-coverage ancient genomes by sequencing a medieval Estonian genome to high depth (23×) and evaluated the performance of its down-sampled replicas. We find that ancient genomes imputed from >0.1× coverage can be reliably used in principal-component analyses without projection. By searching for long shared allele intervals (LSAIs; similar to identity-by-descent segments) in unphased data for >143,000 present-day Estonians, 99 Finns, and 14 imputed ancient genomes from Estonia, we find unexpectedly high levels of individual connectedness between Estonians and Finns for the last eight centuries in contrast to their clear differentiation by allele frequencies. High levels of sharing of these segments between Estonians and Finns predate the demographic expansion and late settlement process of Finland. One plausible source of this extensive sharing is the 8th-10th centuries AD migration event from North Estonia to Finland that has been proposed to explain uniquely shared linguistic features between the Finnish language and the northern dialect of Estonian and shared Christianity-related loanwords from Slavic. These results suggest that LSAI detection provides a computationally tractable way to detect fine-scale structure in large cohorts.},
}

*Alleles
DNA, Ancient/*analysis
Estonia
Female
Finland
Gene Frequency
Genealogy and Heraldry
*Genome, Human
High-Throughput Nucleotide Sequencing
History, 21st Century
History, Ancient
History, Medieval
Human Migration/*history
Humans
Language/history
Male
*Pedigree

@article {pmid34410642,
year = {2021},
author = {Moore, NE and Weyrich, LS},
title = {A Standardized Approach for Shotgun Metagenomic Analysis of Ancient Dental Calculus.},
journal = {Methods in molecular biology (Clifton, N.J.)},
volume = {2327},
number = {},
pages = {93-118},
pmid = {34410642},
issn = {1940-6029},
abstract = {Ancient dental calculus provides a challenging, yet unparalleled, opportunity to reconstruct ancient oral microbial communities and trace the origins of modern microbiota-associated diseases. Metagenomic analysis of ancient dental calculus using high-throughput DNA sequencing has proven itself as an effective method to accurately reconstruct microorganisms that once lived in the mouths of ancient humans. Here, we provide the strategy, methodologies, and approaches used to establish an ancient dental calculus project, from project conception, community engagement, sampling, extracting DNA, and preparing shotgun metagenomic DNA libraries for sequencing on an Illumina platform. We also discuss techniques to minimize background or contaminant DNA by monitoring and reducing contamination in calculus data sets, utilizing appropriate protective gear, and employing the use of sample decontamination strategies. In this methodology chapter, we hope to promote transparency in the ancient dental calculus research field and encourage collaboration across the ancient DNA research community.},
}

@article {pmid34408163,
year = {2021},
author = {Freilich, S and Ringbauer, H and Los, D and Novak, M and Pavičić, DT and Schiffels, S and Pinhasi, R},
title = {Reconstructing genetic histories and social organisation in Neolithic and Bronze Age Croatia.},
journal = {Scientific reports},
volume = {11},
number = {1},
pages = {16729},
pmid = {34408163},
issn = {2045-2322},
support = {HRZZ IP-2016-06-1450//Croatian Science Fund/ ; },
abstract = {Ancient DNA studies have revealed how human migrations from the Neolithic to the Bronze Age transformed the social and genetic structure of European societies. Present-day Croatia lies at the heart of ancient migration routes through Europe, yet our knowledge about social and genetic processes here remains sparse. To shed light on these questions, we report new whole-genome data for 28 individuals dated to between ~ 4700 BCE-400 CE from two sites in present-day eastern Croatia. In the Middle Neolithic we evidence first cousin mating practices and strong genetic continuity from the Early Neolithic. In the Middle Bronze Age community that we studied, we find multiple closely related males suggesting a patrilocal social organisation. We also find in that community an unexpected genetic ancestry profile distinct from individuals found at contemporaneous sites in the region, due to the addition of hunter-gatherer-related ancestry. These findings support archaeological evidence for contacts with communities further north in the Carpathian Basin. Finally, an individual dated to Roman times exhibits an ancestry profile that is broadly present in the region today, adding an important data point to the substantial shift in ancestry that occurred in the region between the Bronze Age and today.},
}

@article {pmid34395085,
year = {2021},
author = {Borry, M and Hübner, A and Rohrlach, AB and Warinner, C},
title = {PyDamage: automated ancient damage identification and estimation for contigs in ancient DNA de novo assembly.},
journal = {PeerJ},
volume = {9},
number = {},
pages = {e11845},
pmid = {34395085},
issn = {2167-8359},
abstract = {DNA de novo assembly can be used to reconstruct longer stretches of DNA (contigs), including genes and even genomes, from short DNA sequencing reads. Applying this technique to metagenomic data derived from archaeological remains, such as paleofeces and dental calculus, we can investigate past microbiome functional diversity that may be absent or underrepresented in the modern microbiome gene catalogue. However, compared to modern samples, ancient samples are often burdened with environmental contamination, resulting in metagenomic datasets that represent mixtures of ancient and modern DNA. The ability to rapidly and reliably establish the authenticity and integrity of ancient samples is essential for ancient DNA studies, and the ability to distinguish between ancient and modern sequences is particularly important for ancient microbiome studies. Characteristic patterns of ancient DNA damage, namely DNA fragmentation and cytosine deamination (observed as C-to-T transitions) are typically used to authenticate ancient samples and sequences, but existing tools for inspecting and filtering aDNA damage either compute it at the read level, which leads to high data loss and lower quality when used in combination with de novo assembly, or require manual inspection, which is impractical for ancient assemblies that typically contain tens to hundreds of thousands of contigs. To address these challenges, we designed PyDamage, a robust, automated approach for aDNA damage estimation and authentication of de novo assembled aDNA. PyDamage uses a likelihood ratio based approach to discriminate between truly ancient contigs and contigs originating from modern contamination. We test PyDamage on both on simulated aDNA data and archaeological paleofeces, and we demonstrate its ability to reliably and automatically identify contigs bearing DNA damage characteristic of aDNA. Coupled with aDNA de novo assembly, Pydamage opens up new doors to explore functional diversity in ancient metagenomic datasets.},
}

@article {pmid34386259,
year = {2021},
author = {Segawa, T and Yonezawa, T and Mori, H and Akiyoshi, A and Allentoft, ME and Kohno, A and Tokanai, F and Willerslev, E and Kohno, N and Nishihara, H},
title = {Ancient DNA reveals multiple origins and migration waves of extinct Japanese brown bear lineages.},
journal = {Royal Society open science},
volume = {8},
number = {8},
pages = {210518},
pmid = {34386259},
issn = {2054-5703},
abstract = {Little is known about how mammalian biogeography on islands was affected by sea-level fluctuations. In the Japanese Archipelago, brown bears (Ursus arctos) currently inhabit only Hokkaido, the northern island, but Pleistocene fossils indicate a past distribution throughout Honshu, Japan's largest island. However, the difficulty of recovering ancient DNA from fossils in temperate East Asia has limited our understanding of their evolutionary history. Here, we analysed mitochondrial DNA from a 32 500-year-old brown bear fossil from Honshu. Our results show that this individual belonged to a previously unknown lineage that split approximately 160 Ka from its sister lineage, the southern Hokkaido clade. This divergence time and fossil record suggest that brown bears migrated from the Eurasian continent to Honshu at least twice; the first population was an early-diverging lineage (greater than 340 Ka), and the second migrated via Hokkaido after approximately 160 Ka, during the ice age. Thus, glacial-age sea-level falls might have facilitated migrations of large mammals more frequently than previously thought, which may have had a substantial impact on ecosystem dynamics in these isolated islands.},
}

@article {pmid34374411,
year = {2021},
author = {Syndercombe Court, D},
title = {Mitochondrial DNA in forensic use.},
journal = {Emerging topics in life sciences},
volume = {5},
number = {3},
pages = {415-426},
doi = {10.1042/ETLS20210204},
pmid = {34374411},
issn = {2397-8554},
abstract = {Genetic analysis of mitochondrial DNA (mtDNA) has always been a useful tool for forensic geneticists, mainly because of its ubiquitous presence in biological material, even in the absence of nuclear DNA. Sequencing, however, is not a skill that is part of the routine forensic analysis because of the relative rarity of requests, and the need for retention of necessary skill sets and associated accreditation issues. While standard Sanger sequencing may be relatively simple, many requests are made in the face of compromised biological samples. Newer technologies, provided through massively parallel sequencing (MPS), will increase the opportunity for scientists to include this tool in their routine, particularly for missing person investigations. MPS has also enabled a different approach to sequencing that can increase sensitivity in a more targeted approach. In these circumstances it is likely that only a laboratory that specialises in undertaking forensic mtDNA analysis will be able to take these difficult cases forward, more so because reviews of the literature have revealed significantly high levels of typing errors in publications reporting mtDNA sequences. The forensic community has set out important guidelines, not only in the practical aspects of analysis, but also in the interpretation of that sequence to ensure that accurate comparisons can be made. Analysis of low-level, compromised and ancient DNA is not easy, however, as contamination is extremely difficult to eliminate and circumstances leading to sequencing errors are all too easily introduced. These problems, and solutions, are discussed in the article in relation to several historic cases.},
}

@article {pmid34357024,
year = {2021},
author = {Onley, IR and Moseby, KE and Austin, JJ},
title = {Genomic Approaches for Conservation Management in Australia under Climate Change.},
journal = {Life (Basel, Switzerland)},
volume = {11},
number = {7},
pages = {},
pmid = {34357024},
issn = {2075-1729},
support = {NA//Australian Government Research Training Program Scholarship/ ; 2019-07//Nature Foundation South Australia Grand Start Grant/ ; NA//Biological Society South Australia/Nature Conservation Society of South Australia Conservation Biology Grant/ ; NA//Field Naturalists Society of South Australia Lirabenda Endowment Fund Research Grant/ ; },
abstract = {Conservation genetics has informed threatened species management for several decades. With the advent of advanced DNA sequencing technologies in recent years, it is now possible to monitor and manage threatened populations with even greater precision. Climate change presents a number of threats and challenges, but new genomics data and analytical approaches provide opportunities to identify critical evolutionary processes of relevance to genetic management under climate change. Here, we discuss the applications of such approaches for threatened species management in Australia in the context of climate change, identifying methods of facilitating viability and resilience in the face of extreme environmental stress. Using genomic approaches, conservation management practices such as translocation, targeted gene flow, and gene-editing can now be performed with the express intention of facilitating adaptation to current and projected climate change scenarios in vulnerable species, thus reducing extinction risk and ensuring the protection of our unique biodiversity for future generations. We discuss the current barriers to implementing conservation genomic projects and the efforts being made to overcome them, including communication between researchers and managers to improve the relevance and applicability of genomic studies. We present novel approaches for facilitating adaptive capacity and accelerating natural selection in species to encourage resilience in the face of climate change.},
}

@article {pmid34350829,
year = {2021},
author = {Guzmán-Solís, AA and Villa-Islas, V and Bravo-López, MJ and Sandoval-Velasco, M and Wesp, JK and Gómez-Valdés, JA and Moreno-Cabrera, ML and Meraz, A and Solís-Pichardo, G and Schaaf, P and TenOever, BR and Blanco-Melo, D and Ávila Arcos, MC},
title = {Ancient viral genomes reveal introduction of human pathogenic viruses into Mexico during the transatlantic slave trade.},
journal = {eLife},
volume = {10},
number = {},
pages = {},
pmid = {34350829},
issn = {2050-084X},
support = {/WT_/Wellcome Trust/United Kingdom ; 208934/Z/17/Z/WT_/Wellcome Trust/United Kingdom ; },
abstract = {After the European colonization of the Americas, there was a dramatic population collapse of the Indigenous inhabitants caused in part by the introduction of new pathogens. Although there is much speculation on the etiology of the Colonial epidemics, direct evidence for the presence of specific viruses during the Colonial era is lacking. To uncover the diversity of viral pathogens during this period, we designed an enrichment assay targeting ancient DNA (aDNA) from viruses of clinical importance and applied it to DNA extracts from individuals found in a Colonial hospital and a Colonial chapel (16th-18th century) where records suggest that victims of epidemics were buried during important outbreaks in Mexico City. This allowed us to reconstruct three ancient human parvovirus B19 genomes and one ancient human hepatitis B virus genome from distinct individuals. The viral genomes are similar to African strains, consistent with the inferred morphological and genetic African ancestry of the hosts as well as with the isotopic analysis of the human remains, suggesting an origin on the African continent. This study provides direct molecular evidence of ancient viruses being transported to the Americas during the transatlantic slave trade and their subsequent introduction to New Spain. Altogether, our observations enrich the discussion about the etiology of infectious diseases during the Colonial period in Mexico.},
}

@article {pmid34343942,
year = {2021},
author = {Rahmat, RA and Humphries, MA and Linacre, AMT and Malik, A and Saedon, NA and Austin, JJ},
title = {Freeze-drying improves DNA yield from teeth.},
journal = {Forensic science international},
volume = {326},
number = {},
pages = {110938},
doi = {10.1016/j.forsciint.2021.110938},
pmid = {34343942},
issn = {1872-6283},
abstract = {The common method of preparing teeth prior to DNA extraction involves cleaning, decontamination, drying and pulverisation. Moisture in post-mortem teeth can promote bacterial growth and hydrolytic damage that could contribute to DNA degradation, whilst also possibly reducing the efficiency of sample pulverisation and DNA release. Here we compared DNA extraction from pig teeth, with- and without freeze-drying, to examine the impact of removing moisture on DNA yield. Quantitative real-time polymerase chain reaction (qPCR) was used to quantify an 83 bp mitochondrial DNA fragment and two nuclear DNA fragments of 82 bp and 150 bp. The comparative results showed that sample preparation with freeze-drying resulted in a higher DNA yield without compromising the DNA quality. This study highlights the advantage of incorporating a freeze-drying to improve the DNA yield and minimising the loss of DNA during sample preparation of teeth.},
}

@article {pmid34335597,
year = {2021},
author = {Haller, M and Bonczarowska, JH and Rieger, D and Lenz, TL and Nebel, A and Krause-Kyora, B},
title = {Ancient DNA Study in Medieval Europeans Shows an Association Between HLA-DRB1*03 and Paratyphoid Fever.},
journal = {Frontiers in immunology},
volume = {12},
number = {},
pages = {691475},
pmid = {34335597},
issn = {1664-3224},
abstract = {Outbreaks of infectious diseases repeatedly affected medieval Europe, leaving behind a large number of dead often inhumed in mass graves. Human remains interred in two burial pits from 14th century CE Germany exhibited molecular evidence of Salmonella enterica Paratyphi C (S. Paratyphi C) infection. The pathogen is responsible for paratyphoid fever, which was likely the cause of death for the buried individuals. This finding presented the unique opportunity to conduct a paratyphoid fever association study in a European population. We focused on HLA-DRB1*03:01 that is a known risk allele for enteric fever in present-day South Asians. We generated HLA profiles for 29 medieval S. Paratyphi C cases and 24 contemporaneous controls and compared these to a modern German population. The frequency of the risk allele was higher in the medieval cases (29.6%) compared to the contemporaneous controls (13%; p = 0.189), albeit not significantly so, possibly because of small sample sizes. Indeed, in comparison with the modern controls (n = 39,689; 10.2%; p = 0.005) the frequency difference became statistically significant. This comparison also suggested a slight decrease in the allele's prevalence between the medieval and modern controls. Up to now, this is the first study on the genetic predisposition to Salmonella infection in Europeans and the only association analysis on paratyphoid fever C. Functional investigation using computational binding prediction between HLA variants and S. Paratyphi and S. Typhi peptides supported a reduced recognition capacity of bacterial proteins by DRB1*03:01 relative to other common DRB1 variants. This pattern could potentially explain the disease association. Our results suggest a slightly reduced predisposition to paratyphoid fever in modern Europeans. The causative allele, however, is still common today, which can be explained by a trade-off, as DRB1*03:01 is protective against infectious respiratory diseases such as severe respiratory syndrome (SARS). It is thus possible that the allele also provided resistance to corona-like viruses in the past.},
}

@article {pmid34332322,
year = {2021},
author = {Wang, M and He, G and Zou, X and Liu, J and Ye, Z and Ming, T and Du, W and Wang, Z and Hou, Y},
title = {Genetic insights into the paternal admixture history of Chinese Mongolians via high-resolution customized Y-SNP SNaPshot panels.},
journal = {Forensic science international. Genetics},
volume = {54},
number = {},
pages = {102565},
doi = {10.1016/j.fsigen.2021.102565},
pmid = {34332322},
issn = {1878-0326},
abstract = {The Mongolian people, one of the Mongolic-speaking populations, are native to the Mongolian Plateau in North China and southern Siberia. Many ancient DNA studies recently reported extensive population transformations during the Paleolithic to historic periods in this region, while little is known about the paternal genetic legacy of modern geographically different Mongolians. Here, we genotyped 215 Y-chromosomal single nucleotide polymorphisms (Y-SNPs) and 37 Y-chromosomal short tandem repeats (Y-STRs) among 679 Mongolian individuals from Hohhot, Hulunbuir, and Ordos in North China using the AGCU Y37 kit and our developed eight Y-SNP SNaPshot panels (including two panels first reported herein). The C-M130 Y-SNP SNaPshot panel defines 28 subhaplogroups, and the N/O/Q complementary Y-SNP SNaPshot panel defines 30 subhaplogroups of N1b-F2930, N1a1a1a1a3-B197, Q-M242, and O2a2b1a1a1a4a-CTS4658, which improved the resolution our developed Y-SNP SNaPshot panel set and could be applied for dissecting the finer-scale paternal lineages of Mongolic speakers. We found a strong association between Mongolian-prevailing haplogroups and some observed microvariants among the newly generated Y-STR haplotype data, suggesting the possibility of haplogroup prediction based on the distribution of Y-STR haplotypes. We identified three main ancestral sources of the observed Mongolian-dominant haplogroups, including the local lineage of C2*-M217 and incoming lineages from other regions of southern East Asia (O2*-M122, O1b*-P31, and N1*-CTS3750) and western Eurasia (R1*-M173). We also observed DE-M145, D1*-M174, C1*-F3393, G*-M201, I-M170, J*-M304, L-M20, O1a*-M119, and Q*-M242 at relatively low frequencies (< 5.00%), suggesting a complex admixture history between Mongolians and other incoming Eurasians from surrounding regions. Genetic clustering analyses indicated that the studied Mongolians showed close genetic affinities with other Altaic-speaking populations and Sinitic-speaking Hui people. The Y-SNP haplotype/haplogroup-based genetic legacy not only revealed that the stratification among geographically/linguistically/ethnically different Chinese populations was highly consistent with the geographical division and language classification, but also demonstrated that patrilineal genetic materials could provide fine-scale genetic structures among geographically different Mongolian people, suggesting that our developed high-resolution Y-SNP SNaPshot panels have the potential for forensic pedigree searches and biogeographical ancestry inference.},
}

@article {pmid34330702,
year = {2021},
author = {Rijal, DP and Heintzman, PD and Lammers, Y and Yoccoz, NG and Lorberau, KE and Pitelkova, I and Goslar, T and Murguzur, FJA and Salonen, JS and Helmens, KF and Bakke, J and Edwards, ME and Alm, T and Bråthen, KA and Brown, AG and Alsos, IG},
title = {Sedimentary ancient DNA shows terrestrial plant richness continuously increased over the Holocene in northern Fennoscandia.},
journal = {Science advances},
volume = {7},
number = {31},
pages = {},
pmid = {34330702},
issn = {2375-2548},
abstract = {The effects of climate change on species richness are debated but can be informed by the past. Here, we generated a sedimentary ancient DNA dataset covering 10 lakes and applied novel methods for data harmonization. We assessed the impact of Holocene climate changes and nutrients on terrestrial plant richness in northern Fennoscandia. We find that richness increased steeply during the rapidly warming Early Holocene. In contrast to findings from most pollen studies, we show that richness continued to increase thereafter, although the climate was stable, with richness and the regional species pool only stabilizing during the past three millennia. Furthermore, overall increases in richness were greater in catchments with higher soil nutrient availability. We suggest that richness will increase with ongoing warming, especially at localities with high nutrient availability and assuming that human activity remains low in the region, although lags of millennia may be expected.},
}

@article {pmid34312252,
year = {2021},
author = {Ottoni, C and Borić, D and Cheronet, O and Sparacello, V and Dori, I and Coppa, A and Antonović, D and Vujević, D and Price, TD and Pinhasi, R and Cristiani, E},
title = {Tracking the transition to agriculture in Southern Europe through ancient DNA analysis of dental calculus.},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {118},
number = {32},
pages = {},
pmid = {34312252},
issn = {1091-6490},
abstract = {Archaeological dental calculus, or mineralized plaque, is a key tool to track the evolution of oral microbiota across time in response to processes that impacted our culture and biology, such as the rise of farming during the Neolithic. However, the extent to which the human oral flora changed from prehistory until present has remained elusive due to the scarcity of data on the microbiomes of prehistoric humans. Here, we present our reconstruction of oral microbiomes via shotgun metagenomics of dental calculus in 44 ancient foragers and farmers from two regions playing a pivotal role in the spread of farming across Europe-the Balkans and the Italian Peninsula. We show that the introduction of farming in Southern Europe did not alter significantly the oral microbiomes of local forager groups, and it was in particular associated with a higher abundance of the species Olsenella sp. oral taxon 807. The human oral environment in prehistory was dominated by a microbial species, Anaerolineaceae bacterium oral taxon 439, that diversified geographically. A Near Eastern lineage of this bacterial commensal dispersed with Neolithic farmers and replaced the variant present in the local foragers. Our findings also illustrate that major taxonomic shifts in human oral microbiome composition occurred after the Neolithic and that the functional profile of modern humans evolved in recent times to develop peculiar mechanisms of antibiotic resistance that were previously absent.},
}

@article {pmid34312232,
year = {2021},
author = {Lazagabaster, IA and Rovelli, V and Fabre, PH and Porat, R and Ullman, M and Davidovich, U and Lavi, T and Ganor, A and Klein, E and Weiss, K and Nuriel, P and Meiri, M and Marom, N},
title = {Rare crested rat subfossils unveil Afro-Eurasian ecological corridors synchronous with early human dispersals.},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {118},
number = {31},
pages = {},
pmid = {34312232},
issn = {1091-6490},
abstract = {Biotic interactions between Africa and Eurasia across the Levant have invoked particular attention among scientists aiming to unravel early human dispersals. However, it remains unclear whether behavioral capacities enabled early modern humans to surpass the Saharo-Arabian deserts or if climatic changes triggered punctuated dispersals out of Africa. Here, we report an unusual subfossil assemblage discovered in a Judean Desert's cliff cave near the Dead Sea and dated to between ∼42,000 and at least 103,000 y ago. Paleogenomic and morphological comparisons indicate that the specimens belong to an extinct subspecies of the eastern African crested rat, Lophiomys imhausi maremortum subspecies nova, which diverged from the modern eastern African populations in the late Middle Pleistocene ∼226,000 to 165,000 y ago. The reported paleomitogenome is the oldest so far in the Levant, opening the door for future paleoDNA analyses in the region. Species distribution modeling points to the presence of continuous habitat corridors connecting eastern Africa with the Levant during the Last Interglacial ∼129,000 to 116,000 y ago, providing further evidence of the northern ingression of African biomes into Eurasia and reinforcing previous suggestions of the critical role of climate change in Late Pleistocene intercontinental biogeography. Furthermore, our study complements other paleoenvironmental proxies with local-instead of interregional-paleoenvironmental data, opening an unprecedented window into the Dead Sea rift paleolandscape.},
}

@article {pmid34308549,
year = {2021},
author = {Juras, A and Ehler, E and Chyleński, M and Pospieszny, Ł and Spinek, AE and Malmström, H and Krzewińska, M and Szostek, K and Pasterkiewicz, W and Florek, M and Wilk, S and Mnich, B and Kruk, J and Szmyt, M and Kozieł, S and Götherström, A and Jakobsson, M and Dabert, M},
title = {Maternal genetic origin of the late and final Neolithic human populations from present-day Poland.},
journal = {American journal of physical anthropology},
volume = {176},
number = {2},
pages = {223-236},
doi = {10.1002/ajpa.24372},
pmid = {34308549},
issn = {1096-8644},
abstract = {OBJECTIVE: We aim to identify maternal genetic affinities between the Middle to Final Neolithic (3850-2300 BC) populations from present-day Poland and possible genetic influences from the Pontic steppe.

MATERIALS AND METHODS: We conducted ancient DNA studies from populations associated with Złota, Globular Amphora, Funnel Beaker, and Corded Ware cultures (CWC). We sequenced genomic libraries on Illumina platform to generate 86 complete ancient mitochondrial genomes. Some of the samples were enriched for mitochondrial DNA using hybridization capture.

RESULTS: The maternal genetic composition found in Złota-associated individuals resembled that found in people associated with the Globular Amphora culture which indicates that both groups likely originated from the same maternal genetic background. Further, these two groups were closely related to the Funnel Beaker culture-associated population. None of these groups shared a close affinity to CWC-associated people. Haplogroup U4 was present only in the CWC group and absent in Złota group, Globular Amphora, and Funnel Beaker cultures.

DISCUSSION: The prevalence of mitochondrial haplogroups of Neolithic farmer origin identified in Early, Middle and Late Neolithic populations suggests a genetic continuity of these maternal lineages in the studied area. Although overlapping in time - and to some extent - in cultural expressions, none of the studied groups (Złota, Globular Amphora, Funnel Beaker), shared a close genetic affinity to CWC-associated people, indicating a larger extent of cultural influence from the Pontic steppe than genetic exchange. The higher frequency of haplogroup U5b found in populations associated with Funnel Beaker, Globular Amphora, and Złota cultures suggest a gradual maternal genetic influx from Mesolithic hunter-gatherers. Moreover, presence of haplogroup U4 in Corded Ware groups is most likely associated with the migrations from the Pontic steppe at the end of the Neolithic and supports the observed genetic distances.},
}

@article {pmid34303876,
year = {2021},
author = {Gerussi, A and Carbone, M and Corpechot, C and Schramm, C and Asselta, R and Invernizzi, P},
title = {The genetic architecture of primary biliary cholangitis.},
journal = {European journal of medical genetics},
volume = {64},
number = {9},
pages = {104292},
doi = {10.1016/j.ejmg.2021.104292},
pmid = {34303876},
issn = {1878-0849},
mesh = {Animals ; *Genetic Predisposition to Disease ; Humans ; Liver Cirrhosis, Biliary/*genetics/pathology ; Multifactorial Inheritance ; },
abstract = {Primary biliary cholangitis (PBC) is a rare autoimmune disease of the liver affecting the small bile ducts. From a genetic point of view, PBC is a complex trait and several genetic and environmental factors have been called in action to explain its etiopathogenesis. Similarly to other complex traits, PBC has benefited from the introduction of genome-wide association studies (GWAS), which identified many variants predisposing or protecting toward the development of the disease. While a progressive endeavour toward the characterization of candidate loci and downstream pathways is currently ongoing, there is still a relatively large portion of heritability of PBC to be revealed. In addition, genetic variation behind progression of the disease and therapeutic response are mostly to be investigated yet. This review outlines the state-of-the-art regarding the genetic architecture of PBC and provides some hints for future investigations, focusing on the study of gene-gene interactions, the application of whole-genome sequencing techniques, and the investigation of X chromosome that can be helpful to cover the missing heritability gap in PBC.},
}

Animals
*Genetic Predisposition to Disease
Humans
Liver Cirrhosis, Biliary/*genetics/pathology
Multifactorial Inheritance

@article {pmid34302465,
year = {2021},
author = {Wang, W and Han, GZ},
title = {Ancient Adaptative Evolution of ACE2 in East Asians.},
journal = {Genome biology and evolution},
volume = {13},
number = {8},
pages = {},
doi = {10.1093/gbe/evab173},
pmid = {34302465},
issn = {1759-6653},
mesh = {Adaptation, Physiological ; Angiotensin-Converting Enzyme 2/*genetics ; Asian Continental Ancestry Group/*genetics ; DNA, Ancient ; *Evolution, Molecular ; Haplotypes ; Humans ; Selection, Genetic ; },
abstract = {Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been posing an unprecedented challenge to global public health. SARS-CoV-2 and several other coronaviruses utilize angiotensin-converting enzyme 2 (ACE2) as their entry receptors. The ACE2 gene has been found to experience episodic positive selection across mammals. However, much remains unknown about how the ACE2 gene evolved in human populations. Here, we use population genetics approaches to investigate the evolution of the ACE2 gene in 26 human populations sampled globally. We find the ACE2 gene exhibits an extremely low nucleotide diversity in the East Asian populations. Strong signals of selective sweep are detected in the East Asian populations, but not in the other human populations. The selective sweep in ACE2 is estimated to begin in East Asian populations ∼23,600 years ago. Our study provides novel insights into the evolution of the ACE2 gene within human populations.},
}

Adaptation, Physiological
Angiotensin-Converting Enzyme 2/*genetics
Asian Continental Ancestry Group/*genetics
DNA, Ancient
*Evolution, Molecular
Haplotypes
Humans
Selection, Genetic

@article {pmid34301878,
year = {2021},
author = {Mitchell, KJ},
title = {Ancient DNA from the koala lemur puts Madagascar on the paleogenomic map.},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {118},
number = {30},
pages = {},
pmid = {34301878},
issn = {1091-6490},
}

@article {pmid34294811,
year = {2021},
author = {Rohrlach, AB and Papac, L and Childebayeva, A and Rivollat, M and Villalba-Mouco, V and Neumann, GU and Penske, S and Skourtanioti, E and van de Loosdrecht, M and Akar, M and Boyadzhiev, K and Boyadzhiev, Y and Deguilloux, MF and Dobeš, M and Erdal, YS and Ernée, M and Frangipane, M and Furmanek, M and Friederich, S and Ghesquière, E and Hałuszko, A and Hansen, S and Küßner, M and Mannino, M and Özbal, R and Reinhold, S and Rottier, S and Salazar-García, DC and Diaz, JS and Stockhammer, PW and de Togores Muñoz, CR and Yener, KA and Posth, C and Krause, J and Herbig, A and Haak, W},
title = {Using Y-chromosome capture enrichment to resolve haplogroup H2 shows new evidence for a two-path Neolithic expansion to Western Europe.},
journal = {Scientific reports},
volume = {11},
number = {1},
pages = {15005},
pmid = {34294811},
issn = {2045-2322},
support = {771234 - PALEoRIDER//Deutsche Forschungsgemeinschaft/ ; ANR-17-FRAL-0010//Agence Nationale de la Recherche/ ; RVO 67985912//Akademie Věd České Republiky/ ; },
abstract = {Uniparentally-inherited markers on mitochondrial DNA (mtDNA) and the non-recombining regions of the Y chromosome (NRY), have been used for the past 30 years to investigate the history of humans from a maternal and paternal perspective. Researchers have preferred mtDNA due to its abundance in the cells, and comparatively high substitution rate. Conversely, the NRY is less susceptible to back mutations and saturation, and is potentially more informative than mtDNA owing to its longer sequence length. However, due to comparatively poor NRY coverage via shotgun sequencing, and the relatively low and biased representation of Y-chromosome variants on capture assays such as the 1240 k, ancient DNA studies often fail to utilize the unique perspective that the NRY can yield. Here we introduce a new DNA enrichment assay, coined YMCA (Y-mappable capture assay), that targets the "mappable" regions of the NRY. We show that compared to low-coverage shotgun sequencing and 1240 k capture, YMCA significantly improves the mean coverage and number of sites covered on the NRY, increasing the number of Y-haplogroup informative SNPs, and allowing for the identification of previously undiscovered variants. To illustrate the power of YMCA, we show that the analysis of ancient Y-chromosome lineages can help to resolve Y-chromosomal haplogroups. As a case study, we focus on H2, a haplogroup associated with a critical event in European human history: the Neolithic transition. By disentangling the evolutionary history of this haplogroup, we further elucidate the two separate paths by which early farmers expanded from Anatolia and the Near East to western Europe.},
}

@article {pmid34283930,
year = {2021},
author = {Grewe, F and Kronforst, MR and Pierce, NE and Moreau, CS},
title = {Museum genomics reveals the Xerces blue butterfly (Glaucopsyche xerces) was a distinct species driven to extinction.},
journal = {Biology letters},
volume = {17},
number = {7},
pages = {20210123},
pmid = {34283930},
issn = {1744-957X},
support = {R35 GM131828/GM/NIGMS NIH HHS/United States ; },
mesh = {Animals ; *Butterflies/genetics ; *Extinction, Biological ; *Genome, Mitochondrial ; Genomics ; Museums ; Phylogeny ; Sequence Analysis, DNA ; },
abstract = {The last Xerces blue butterfly was seen in the early 1940s, and its extinction is credited to human urban development. This butterfly has become a North American icon for insect conservation, but some have questioned whether it was truly a distinct species, or simply an isolated population of another living species. To address this question, we leveraged next-generation sequencing using a 93-year-old museum specimen. We applied a genome skimming strategy that aimed for the organellar genome and high-copy fractions of the nuclear genome by a shallow sequencing approach. From these data, we were able to recover over 200 million nucleotides, which assembled into several phylogenetically informative markers and the near-complete mitochondrial genome. From our phylogenetic analyses and haplotype network analysis we conclude that the Xerces blue butterfly was a distinct species driven to extinction.},
}

Animals
*Butterflies/genetics
*Extinction, Biological
*Genome, Mitochondrial
Genomics
Museums
Phylogeny
Sequence Analysis, DNA

@article {pmid34265517,
year = {2021},
author = {Hofreiter, M and Sneberger, J and Pospisek, M and Vanek, D},
title = {Progress in forensic bone DNA analysis: Lessons learned from ancient DNA.},
journal = {Forensic science international. Genetics},
volume = {54},
number = {},
pages = {102538},
doi = {10.1016/j.fsigen.2021.102538},
pmid = {34265517},
issn = {1878-0326},
abstract = {Research on ancient and forensic DNA is related in many ways, and the two fields must deal with similar obstacles. Therefore, communication between these two communities has the potential to improve results in both research fields. Here, we present the insights gained in the ancient DNA community with regard to analyzing DNA from aged skeletal material and the potential use of the developed protocols in forensic work. We discuss the various steps, from choosing samples for DNA extraction to deciding between classical PCR amplification and massively parallel sequencing approaches. Based on the progress made in ancient DNA analyses combined with the requirements of forensic work, we suggest that there is substantial potential for incorporating ancient DNA approaches into forensic protocols, a process that has already begun to a considerable extent. However, taking full advantage of the experiences gained from ancient DNA work will require comparative studies by the forensic DNA community to tailor the methods developed for ancient samples to the specific needs of forensic studies and case work. If successful, in our view, the benefits for both communities would be considerable.},
}

@article {pmid34256582,
year = {2021},
author = {Rossi, C and Ruß-Popa, G and Mattiangeli, V and McDaid, F and Hare, AJ and Davoudi, H and Laleh, H and Lorzadeh, Z and Khazaeli, R and Fathi, H and Teasdale, MD and A'ali, A and Stöllner, T and Mashkour, M and Daly, KG},
title = {Exceptional ancient DNA preservation and fibre remains of a Sasanian saltmine sheep mummy in Chehrābād, Iran.},
journal = {Biology letters},
volume = {17},
number = {7},
pages = {20210222},
pmid = {34256582},
issn = {1744-957X},
mesh = {Animals ; DNA, Ancient ; Genome ; Iran ; *Mummies ; Phenotype ; Sheep/genetics ; },
abstract = {Mummified remains have long attracted interest as a potential source of ancient DNA. However, mummification is a rare process that requires an anhydrous environment to rapidly dehydrate and preserve tissue before complete decomposition occurs. We present the whole-genome sequences (3.94 X) of an approximately 1600-year-old naturally mummified sheep recovered from Chehrābād, a salt mine in northwestern Iran. Comparative analyses of published ancient sequences revealed the remarkable DNA integrity of this mummy. Hallmarks of postmortem damage, fragmentation and hydrolytic deamination are substantially reduced, likely owing to the high salinity of this taphonomic environment. Metagenomic analyses reflect the profound influence of high-salt content on decomposition; its microbial profile is predominated by halophilic archaea and bacteria, possibly contributing to the remarkable preservation of the sample. Applying population genomic analyses, we find clustering of this sheep with Southwest Asian modern breeds, suggesting ancestry continuity. Genotyping of a locus influencing the woolly phenotype showed the presence of an ancestral 'hairy' allele, consistent with hair fibre imaging. This, along with derived alleles associated with the fat-tail phenotype, provides genetic evidence that Sasanian-period Iranians maintained specialized sheep flocks for different uses, with the 'hairy', 'fat-tailed'-genotyped sheep likely kept by the rural community of Chehrābād's miners.},
}

Animals
DNA, Ancient
Genome
Iran
*Mummies
Phenotype
Sheep/genetics

@article {pmid34256019,
year = {2021},
author = {Gelabert, P and Sawyer, S and Bergström, A and Margaryan, A and Collin, TC and Meshveliani, T and Belfer-Cohen, A and Lordkipanidze, D and Jakeli, N and Matskevich, Z and Bar-Oz, G and Fernandes, DM and Cheronet, O and Özdoğan, KT and Oberreiter, V and Feeney, RNM and Stahlschmidt, MC and Skoglund, P and Pinhasi, R},
title = {Genome-scale sequencing and analysis of human, wolf, and bison DNA from 25,000-year-old sediment.},
journal = {Current biology : CB},
volume = {31},
number = {16},
pages = {3564-3574.e9},
pmid = {34256019},
issn = {1879-0445},
support = {FC001595/MRC_/Medical Research Council/United Kingdom ; 852558/ERC_/European Research Council/International ; FC001595/WT_/Wellcome Trust/United Kingdom ; FC001595/ARC_/Arthritis Research UK/United Kingdom ; FC001595/CRUK_/Cancer Research UK/United Kingdom ; /WT_/Wellcome Trust/United Kingdom ; },
abstract = {Cave sediments have been shown to preserve ancient DNA but so far have not yielded the genome-scale information of skeletal remains. We retrieved and analyzed human and mammalian nuclear and mitochondrial environmental "shotgun" genomes from a single 25,000-year-old Upper Paleolithic sediment sample from Satsurblia cave, western Georgia:first, a human environmental genome with substantial basal Eurasian ancestry, which was an ancestral component of the majority of post-Ice Age people in the Near East, North Africa, and parts of Europe; second, a wolf environmental genome that is basal to extant Eurasian wolves and dogs and represents a previously unknown, likely extinct, Caucasian lineage; and third, a European bison environmental genome that is basal to present-day populations, suggesting that population structure has been substantially reshaped since the Last Glacial Maximum. Our results provide new insights into the Late Pleistocene genetic histories of these three species and demonstrate that direct shotgun sequencing of sediment DNA, without target enrichment methods, can yield genome-wide data informative of ancestry and phylogenetic relationships.},
}

@article {pmid34242562,
year = {2021},
author = {Pinhasi, R and Douka, K},
title = {Before and after farming: The genetic structure of South China and Southeast Asia.},
journal = {Cell},
volume = {184},
number = {14},
pages = {3597-3598},
doi = {10.1016/j.cell.2021.06.016},
pmid = {34242562},
issn = {1097-4172},
abstract = {In this issue of Cell, Wang et al. harness ancient DNA methods to produce and analyze new genomic data from 31 individuals from South China, dated between 500 and 10,000-12,000 years ago. The study reveals a complex interplay between groups of three different genetic ancestries and provides a new perspective on interactions and agricultural dispersals in South China and Southeast Asia.},
}

@article {pmid34213855,
year = {2021},
author = {Rotival, M and Cossart, P and Quintana-Murci, L},
title = {Reconstructing 50,000 years of human history from our DNA: lessons from modern genomics.},
journal = {Comptes rendus biologies},
volume = {344},
number = {2},
pages = {177-187},
doi = {10.5802/crbiol.55},
pmid = {34213855},
issn = {1768-3238},
mesh = {Africa ; Animals ; Archaeology ; *DNA ; Dogs ; Genome ; Genome, Human ; *Genomics ; History, Ancient ; Horses ; Humans ; },
abstract = {The advent of high throughput sequencing approaches and ancient DNA techniques have enabled reconstructing the history of human populations at an unprecedented level of resolution. The symposium from the French Academy of Sciences "50,000 ans d'épopée humaine dans notre ADN" has reviewed some of the latest contributions from the fields of genomics, archaeology, and linguistics to our understanding of >300,000 years of human history. DNA has revealed the richness of the human journey, from the deep divergences between human populations in Africa, to the first encounters of Homo Sapiens with other hominins on their way to Eurasia and the peopling of Remote Oceania. The symposium has also emphasized how migrations, cultural practices, and environmental pathogens have contributed to shape the genetic diversity of modern humans, through admixture, genetic drift or genetic adaptation. Finally, special attention was also given to how human behaviours have shaped the genome of other species, through the spreading of microbes and pathogens, as in the case of Yersinia Pestis, or through domestication, as elegantly demonstrated for dogs, horses, and apples. Altogether, this conference illustrated how the complex history of human populations is tightly linked with their contemporary genetic diversity that, in turn, has direct effects on their identity and health.},
}

Africa
Animals
Archaeology
*DNA
Dogs
Genome
Genome, Human
*Genomics
History, Ancient
Horses
Humans

@article {pmid34210535,
year = {2021},
author = {de Vareilles, A and Pelling, R and Woodbridge, J and Fyfe, R},
title = {Archaeology and agriculture: plants, people, and past land-use.},
journal = {Trends in ecology & evolution},
volume = {36},
number = {10},
pages = {943-954},
doi = {10.1016/j.tree.2021.06.003},
pmid = {34210535},
issn = {1872-8383},
abstract = {As a specialised branch of archaeology requiring specific field and laboratory methodologies, the contributions of archaeobotany have often been overlooked by the ecological research community. Developments in the fields of botany, chemistry, and ancient DNA analyses have greatly increased the potential for archaeobotany to contribute to topical questions relating to the Anthropocene and landscape transformations. We review the role of archaeobotany in identifying and describing past arable land use. Analytical techniques are illustrated with examples at both local and regional scales, demonstrating how archaeobotany can provide unique details of the wide array of past subsistence and land-use strategies. These data and their potential should be better recognised as important information that could underpin models seeking to evaluate or predict the effects of socioenvironmental interactions.},
}

@article {pmid34208224,
year = {2021},
author = {Vai, S and Diroma, MA and Cannariato, C and Budnik, A and Lari, M and Caramelli, D and Pilli, E},
title = {How a Paleogenomic Approach Can Provide Details on Bioarchaeological Reconstruction: A Case Study from the Globular Amphorae Culture.},
journal = {Genes},
volume = {12},
number = {6},
pages = {},
pmid = {34208224},
issn = {2073-4425},
mesh = {Archaeology/*methods ; Biological Evolution ; *DNA, Ancient ; Genome, Human ; Genomics/*methods ; Humans ; Pedigree ; Social Evolution ; },
abstract = {Ancient human remains have the potential to explain a great deal about the prehistory of humankind. Due to recent technological and bioinformatics advances, their study, at the palaeogenomic level, can provide important information about population dynamics, culture changes, and the lifestyles of our ancestors. In this study, mitochondrial and nuclear genome data obtained from human bone remains associated with the Neolithic Globular Amphorae culture, which were recovered in the Megalithic barrow of Kierzkowo (Poland), were reanalysed to gain insight into the social organisation and use of the archaeological site and to provide information at the individual level. We were able to successfully estimate the minimum number of individuals, sex, kin relationships, and phenotypic traits of the buried individuals, despite the low level of preservation of the bone samples and the intricate taphonomic conditions. In addition, the evaluation of damage patterns allowed us to highlight the presence of "intruders"-that is, of more recent skeletal remains that did not belong to the original burial. Due to its characteristics, the study of the Kierzkowo barrow represented a challenge for the reconstruction of the biological profile of the human community who exploited it and an excellent example of the contribution that ancient genomic analysis can provide to archaeological reconstruction.},
}

Archaeology/*methods
Biological Evolution
*DNA, Ancient
Genome, Human
Genomics/*methods
Humans
Pedigree
Social Evolution

@article {pmid34206575,
year = {2021},
author = {Klecel, W and Martyniuk, E},
title = {From the Eurasian Steppes to the Roman Circuses: A Review of Early Development of Horse Breeding and Management.},
journal = {Animals : an open access journal from MDPI},
volume = {11},
number = {7},
pages = {},
pmid = {34206575},
issn = {2076-2615},
abstract = {The domestication of the horse began about 5500 years ago in the Eurasian steppes. In the following millennia horses spread across the ancient world, and their role in transportation and warfare affected every ancient culture. Ownership of horses became an indicator of wealth and social status. The importance of horses led to a growing interest in their breeding and management. Many phenotypic traits, such as height, behavior, and speed potential, have been proven to be a subject of selection; however, the details of ancient breeding practices remain mostly unknown. From the fourth millennium BP, through the Iron Age, many literature sources thoroughly describe horse training systems, as well as various aspects of husbandry, many of which are still in use today. The striking resemblance of ancient and modern equine practices leaves us wondering how much was accomplished through four thousand years of horse breeding.},
}

@article {pmid34202821,
year = {2021},
author = {Purnomo, GA and Mitchell, KJ and O'Connor, S and Kealy, S and Taufik, L and Schiller, S and Rohrlach, A and Cooper, A and Llamas, B and Sudoyo, H and Teixeira, JC and Tobler, R},
title = {Mitogenomes Reveal Two Major Influxes of Papuan Ancestry across Wallacea Following the Last Glacial Maximum and Austronesian Contact.},
journal = {Genes},
volume = {12},
number = {7},
pages = {},
pmid = {34202821},
issn = {2073-4425},
support = {IN18010007//Australian Research Council/ ; DE190101069//Australian Research Council/ ; //Indonesian Ministry of Research and Technology/National Research and Innovation Agency/ ; },
abstract = {The tropical archipelago of Wallacea contains thousands of individual islands interspersed between mainland Asia and Near Oceania, and marks the location of a series of ancient oceanic voyages leading to the peopling of Sahul-i.e., the former continent that joined Australia and New Guinea at a time of lowered sea level-by 50,000 years ago. Despite the apparent deep antiquity of human presence in Wallacea, prior population history research in this region has been hampered by patchy archaeological and genetic records and is largely concentrated upon more recent history that follows the arrival of Austronesian seafarers ~3000-4000 years ago (3-4 ka). To shed light on the deeper history of Wallacea and its connections with New Guinea and Australia, we performed phylogeographic analyses on 656 whole mitogenomes from these three regions, including 186 new samples from eight Wallacean islands and three West Papuan populations. Our results point to a surprisingly dynamic population history in Wallacea, marked by two periods of extensive demographic change concentrated around the Last Glacial Maximum ~15 ka and post-Austronesian contact ~3 ka. These changes appear to have greatly diminished genetic signals informative about the original peopling of Sahul, and have important implications for our current understanding of the population history of the region.},
}

@article {pmid34202264,
year = {2021},
author = {Baldoni, M and Nardi, A and De Angelis, F and Rickards, O and Martínez-Labarga, C},
title = {How Does Diet Influence Our Lives? Evaluating the Relationship between Isotopic Signatures and Mortality Patterns in Italian Roman Imperial and Medieval Periods.},
journal = {Molecules (Basel, Switzerland)},
volume = {26},
number = {13},
pages = {},
pmid = {34202264},
issn = {1420-3049},
mesh = {Carbon Isotopes/analysis ; Diet/*history ; History, Ancient ; History, Medieval ; Humans ; Italy ; Mortality/*history ; Nitrogen Isotopes/analysis ; },
abstract = {The present research investigates the relationship between dietary habits and mortality patterns in the Roman Imperial and Medieval periods. The reconstructions of population dynamics and subsistence strategies provide a fascinating source of information for understanding our history. This is particularly true given that the changes in social, economic, political, and religious aspects related to the transition from the Roman period to the Middle Ages have been widely discussed. We analyzed the isotopic and mortality patterns of 616 individuals from 18 archeological sites (the Medieval Latium sites of Colonna, Santa Severa, Allumiere, Cencelle, and 14 Medieval and Imperial funerary contexts from Rome) to compile a survivorship analysis. A semi-parametric approach was applied, suggesting variations in mortality patterns between sexes in the Roman period. Nitrogen isotopic signatures influenced mortality in both periods, showing a quadratic and a linear effect for Roman Imperial and Medieval populations, respectively. No influence of carbon isotopic signatures has been detected for Roman Imperial populations. Conversely, increased mortality risk for rising carbon isotopic values was observed in Medieval samples.},
}

Carbon Isotopes/analysis
Diet/*history
History, Ancient
History, Medieval
Humans
Italy
Mortality/*history
Nitrogen Isotopes/analysis

@article {pmid34198074,
year = {2021},
author = {Sahoo, S and Samal, R and Biswas, S},
title = {Efficacy of a novel bone preprocessing method for better DNA yield.},
journal = {Forensic science international},
volume = {325},
number = {},
pages = {110887},
doi = {10.1016/j.forsciint.2021.110887},
pmid = {34198074},
issn = {1872-6283},
mesh = {DNA/*analysis ; *DNA Fingerprinting ; Femur/*chemistry ; Forensic Genetics/methods ; Humans ; *Microsatellite Repeats ; Multiplex Polymerase Chain Reaction ; Specimen Handling/*methods ; },
abstract = {In cases involving identification of missing persons, mass disasters and ancient DNA investigations, bone and teeth samples are often the only, and almost always the best, biological material available for DNA profiling. Standard methods for extraction of DNA from such samples involve grinding of the bone and teeth samples. Here, we present an extremely efficient protocol for recovery of DNA from bone samples by a method of scrapping. The study was carried out on 25 samples and it was found that the quantity of DNA isolated by the scrapping method was up to 1.131 ng/µl with a success rate of 93% as compared to a much lower yield of 0.359 ng/µl DNA isolated with a success rate of 28% through the grinding method. The scrapping method of DNA extraction has been proven to be extremely useful in forensic examination of challenging samples that had multiple failures using the traditional grinding method.},
}

DNA/*analysis
*DNA Fingerprinting
Femur/*chemistry
Forensic Genetics/methods
Humans
*Microsatellite Repeats
Multiplex Polymerase Chain Reaction
Specimen Handling/*methods

@article {pmid34191795,
year = {2021},
author = {Ingman, T and Eisenmann, S and Skourtanioti, E and Akar, M and Ilgner, J and Gnecchi Ruscone, GA and le Roux, P and Shafiq, R and Neumann, GU and Keller, M and Freund, C and Marzo, S and Lucas, M and Krause, J and Roberts, P and Yener, KA and Stockhammer, PW},
title = {Human mobility at Tell Atchana (Alalakh), Hatay, Turkey during the 2nd millennium BC: Integration of isotopic and genomic evidence.},
journal = {PloS one},
volume = {16},
number = {6},
pages = {e0241883},
pmid = {34191795},
issn = {1932-6203},
abstract = {The Middle and Late Bronze Age, a period roughly spanning the 2nd millennium BC (ca. 2000-1200 BC) in the Near East, is frequently referred to as the first 'international age', characterized by intense and far-reaching contacts between different entities from the eastern Mediterranean to the Near East and beyond. In a large-scale tandem study of stable isotopes and ancient DNA of individuals excavated at Tell Atchana (Alalakh, located in Hatay, Turkey), we explored the role of mobility at the capital of a regional kingdom, named Mukish during the Late Bronze Age, which spanned the Amuq Valley and some areas beyond. We generated strontium and oxygen isotope data from dental enamel for 53 individuals and 77 individuals, respectively, and added ancient DNA data of 10 newly sequenced individuals to a dataset of 27 individuals published in 2020. Additionally, we improved the DNA coverage of one individual from this 2020 dataset. The DNA data revealed a very homogeneous gene pool. This picture of an overwhelmingly local ancestry was consistent with the evidence of local upbringing in most of the individuals indicated by the isotopic data, where only five were found to be non-local. High levels of contact, trade, and exchange of ideas and goods in the Middle and Late Bronze Ages, therefore, seem not to have translated into high levels of individual mobility detectable at Tell Atchana.},
}

@article {pmid34187204,
year = {2021},
author = {Euskirchen, A and Hartmann, L and Mazanec, J and Wittmeier, P and Hummel, S},
title = {The influence of sample quantity and lysis parameters on the success of ancient DNA extraction from skeletal remains.},
journal = {BioTechniques},
volume = {71},
number = {1},
pages = {376-381},
doi = {10.2144/btn-2020-0169},
pmid = {34187204},
issn = {1940-9818},
abstract = {DNA extraction is of utmost importance in archaeobiology, as it determines the success of further DNA analyses. This study concentrates on the success of ancient DNA extraction using silica spin columns and PCR-based analysis from archaeological skeletal material and investigates the influence of sample quantity, lysis time and lysis temperature during sample preparation. The results show that lysis times ranging from 2 to 48 h are suitable, and that lysis should be carried out at a constant temperature of 56°C. Concerning sample quantity, 10 mg for mitochondrial DNA and 50 mg for chromosomal DNA are sufficient for high quality analyses. Thus invaluable sample material can be saved, and time of sample preparation can be reduced considerably.},
}

@article {pmid34184252,
year = {2021},
author = {Bisso-Machado, R and Fagundes, NJR},
title = {Uniparental genetic markers in Native Americans: A summary of all available data from ancient and contemporary populations.},
journal = {American journal of physical anthropology},
volume = {},
number = {},
pages = {},
doi = {10.1002/ajpa.24357},
pmid = {34184252},
issn = {1096-8644},
support = {Finance Code 001//Coordenação de Aperfeiçoamento de Pessoal de Nível Superior/ ; },
abstract = {OBJECTIVES: The aim of this study was to create a comprehensive summary of available mtDNA and Y-chromosome data for Native Americans from North, Central, and South America, including both modern and ancient DNA. To illustrate the usefulness of this dataset we present a broad picture of the genetic variation for both markers across the Americas.

METHODS: We searched PubMed, ResearchGate, Google Scholar for studies about mtDNA or Y-chromosome variation in Native American populations, including geographic, linguistic, ecological (ecoregion), archeological and chronological information. We used AMOVA to estimate the genetic structure associated with language and ecoregion grouping and Mantel tests to evaluate the correlation between genetic and geographic distances.

RESULTS: Genetic data were obtained from 321 primary sources, including 22,569 individuals from 298 contemporary populations, and 3628 individuals from 202 archeological populations. MtDNA lineages of probable non-Amerindian origin were rare, in contrast with Y-chromosome lineages. Mantel tests showed a statistically significant correlation for the whole continent considering mtDNA but not the Y-chromosome. Genetic structure between groups was always stronger for mtDNA than for the Y-chromosome.

CONCLUSIONS: This study summarizes decades of research conducted in Native American populations for both mtDNA and the Y-chromosome. Continental or sub-continental patterns of variation reveal that most of the genetic variation occurs within populations rather than among linguistic or ecoregional groups, and that isolation by distance is barely detectable in most population sets. The genetic structure among groups was always larger for mtDNA than for the Y-chromosome, suggesting between-sex differences in gene flow.},
}

@article {pmid34181791,
year = {2021},
author = {Sacks, BN and Mitchell, KJ and Quinn, CB and Hennelly, LM and Sinding, MS and Statham, MJ and Preckler-Quisquater, S and Fain, SR and Kistler, L and Vanderzwan, SL and Meachen, JA and Ostrander, EA and Frantz, LAF},
title = {Pleistocene origins, western ghost lineages, and the emerging phylogeographic history of the red wolf and coyote.},
journal = {Molecular ecology},
volume = {30},
number = {17},
pages = {4292-4304},
doi = {10.1111/mec.16048},
pmid = {34181791},
issn = {1365-294X},
support = {210119/Z/18/Z/WT_/Wellcome Trust/United Kingdom ; },
mesh = {Animals ; *Coyotes/genetics ; Ecosystem ; Hybridization, Genetic ; Phylogeography ; *Wolves/genetics ; },
abstract = {The red wolf (Canis rufus) of the eastern US was driven to near-extinction by colonial-era persecution and habitat conversion, which facilitated coyote (C. latrans) range expansion and widespread hybridization with red wolves. The observation of some grey wolf (C. lupus) ancestry within red wolves sparked controversy over whether it was historically a subspecies of grey wolf with its predominant "coyote-like" ancestry obtained from post-colonial coyote hybridization (2-species hypothesis) versus a distinct species closely related to the coyote that hybridized with grey wolf (3-species hypothesis). We analysed mitogenomes sourced from before the 20th century bottleneck and coyote invasion, along with hundreds of modern amplicons, which led us to reject the 2-species model and to investigate a broader phylogeographic 3-species model suggested by the fossil record. Our findings broadly support this model, in which red wolves ranged the width of the American continent prior to arrival of the grey wolf to the mid-continent 60-80 ka; red wolves subsequently disappeared from the mid-continent, relegated to California and the eastern forests, which ushered in emergence of the coyote in their place (50-30 ka); by the early Holocene (12-10 ka), coyotes had expanded into California, where they admixed with and phenotypically replaced western red wolves in a process analogous to the 20th century coyote invasion of the eastern forests. Findings indicate that the red wolf pre-dated not only European colonization but human, and possibly coyote, presence in North America. These findings highlight the urgency of expanding conservation efforts for the red wolf.},
}

Animals
*Coyotes/genetics
Ecosystem
Hybridization, Genetic
Phylogeography
*Wolves/genetics

@article {pmid34171307,
year = {2021},
author = {Wang, T and Wang, W and Xie, G and Li, Z and Fan, X and Yang, Q and Wu, X and Cao, P and Liu, Y and Yang, R and Liu, F and Dai, Q and Feng, X and Wu, X and Qin, L and Li, F and Ping, W and Zhang, L and Zhang, M and Liu, Y and Chen, X and Zhang, D and Zhou, Z and Wu, Y and Shafiey, H and Gao, X and Curnoe, D and Mao, X and Bennett, EA and Ji, X and Yang, MA and Fu, Q},
title = {Human population history at the crossroads of East and Southeast Asia since 11,000 years ago.},
journal = {Cell},
volume = {184},
number = {14},
pages = {3829-3841.e21},
doi = {10.1016/j.cell.2021.05.018},
pmid = {34171307},
issn = {1097-4172},
abstract = {Past human genetic diversity and migration between southern China and Southeast Asia have not been well characterized, in part due to poor preservation of ancient DNA in hot and humid regions. We sequenced 31 ancient genomes from southern China (Guangxi and Fujian), including two ∼12,000- to 10,000-year-old individuals representing the oldest humans sequenced from southern China. We discovered a deeply diverged East Asian ancestry in the Guangxi region that persisted until at least 6,000 years ago. We found that ∼9,000- to 6,000-year-old Guangxi populations were a mixture of local ancestry, southern ancestry previously sampled in Fujian, and deep Asian ancestry related to Southeast Asian Hòabìnhian hunter-gatherers, showing broad admixture in the region predating the appearance of farming. Historical Guangxi populations dating to ∼1,500 to 500 years ago are closely related to Tai-Kadai and Hmong-Mien speakers. Our results show heavy interactions among three distinct ancestries at the crossroads of East and Southeast Asia.},
}

@article {pmid34171302,
year = {2021},
author = {Souilmi, Y and Lauterbur, ME and Tobler, R and Huber, CD and Johar, AS and Moradi, SV and Johnston, WA and Krogan, NJ and Alexandrov, K and Enard, D},
title = {An ancient viral epidemic involving host coronavirus interacting genes more than 20,000 years ago in East Asia.},
journal = {Current biology : CB},
volume = {31},
number = {16},
pages = {3504-3514.e9},
doi = {10.1016/j.cub.2021.05.067},
pmid = {34171302},
issn = {1879-0445},
support = {R01 AI120694/AI/NIAID NIH HHS/United States ; U19 AI135990/AI/NIAID NIH HHS/United States ; R01 AI122747/AI/NIAID NIH HHS/United States ; U19 AI135972/AI/NIAID NIH HHS/United States ; P01 AI063302/AI/NIAID NIH HHS/United States ; P50 AI150476/AI/NIAID NIH HHS/United States ; R01 AI143292/AI/NIAID NIH HHS/United States ; },
mesh = {Coronavirus/*genetics ; Coronavirus Infections/*history/virology ; Datasets as Topic ; Evolution, Molecular ; Far East/epidemiology ; Gene Frequency ; Genetic Predisposition to Disease ; Genome, Human/*genetics ; Genome, Viral/genetics ; Genome-Wide Association Study ; History, Ancient ; Host Microbial Interactions/*genetics ; Human Genome Project ; Humans ; Mutation ; Pandemics/*history ; Phylogeny ; Selection, Genetic ; },
abstract = {The current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has emphasized the vulnerability of human populations to novel viral pressures, despite the vast array of epidemiological and biomedical tools now available. Notably, modern human genomes contain evolutionary information tracing back tens of thousands of years, which may help identify the viruses that have impacted our ancestors-pointing to which viruses have future pandemic potential. Here, we apply evolutionary analyses to human genomic datasets to recover selection events involving tens of human genes that interact with coronaviruses, including SARS-CoV-2, that likely started more than 20,000 years ago. These adaptive events were limited to the population ancestral to East Asian populations. Multiple lines of functional evidence support an ancient viral selective pressure, and East Asia is the geographical origin of several modern coronavirus epidemics. An arms race with an ancient coronavirus, or with a different virus that happened to use similar interactions as coronaviruses with human hosts, may thus have taken place in ancestral East Asian populations. By learning more about our ancient viral foes, our study highlights the promise of evolutionary information to better predict the pandemics of the future. Importantly, adaptation to ancient viral epidemics in specific human populations does not necessarily imply any difference in genetic susceptibility between different human populations, and the current evidence points toward an overwhelming impact of socioeconomic factors in the case of coronavirus disease 2019 (COVID-19).},
}

Coronavirus/*genetics
Coronavirus Infections/*history/virology
Datasets as Topic
Evolution, Molecular
Far East/epidemiology
Gene Frequency
Genetic Predisposition to Disease
Genome, Human/*genetics
Genome, Viral/genetics
Genome-Wide Association Study
History, Ancient
Host Microbial Interactions/*genetics
Human Genome Project
Humans
Mutation
Pandemics/*history
Phylogeny
Selection, Genetic

@article {pmid34164993,
year = {2021},
author = {Essel, E and Korlević, P and Meyer, M},
title = {A method for the temperature-controlled extraction of DNA from ancient bones.},
journal = {BioTechniques},
volume = {71},
number = {1},
pages = {382-386},
doi = {10.2144/btn-2021-0025},
pmid = {34164993},
issn = {1940-9818},
support = {//Max-Planck-Gesellschaft/ ; },
abstract = {Contamination with microbial and other exogenous DNA poses a significant challenge in the generation of genome-wide sequence data from ancient skeletal remains. Here we describe a method for separating ancient DNA into multiple fractions during DNA extraction by sequential temperature-controlled release of DNA into sodium phosphate buffer. An evaluation of the effectiveness of the method using a set of three ancient bones resulted in between 1.6- and 32-fold enrichment of endogenous DNA compared with regular DNA extraction. For two bones, the method outperformed previous methods of decontaminating ancient bones, including hypochlorite treatment, which resulted in near-complete destruction of DNA in the worst-preserved sample. This extraction method expands the spectrum of methods available for depleting contaminant DNA from ancient skeletal remains.},
}

@article {pmid34162703,
year = {2021},
author = {Marciniak, S and Mughal, MR and Godfrey, LR and Bankoff, RJ and Randrianatoandro, H and Crowley, BE and Bergey, CM and Muldoon, KM and Randrianasy, J and Raharivololona, BM and Schuster, SC and Malhi, RS and Yoder, AD and Louis, EE and Kistler, L and Perry, GH},
title = {Evolutionary and phylogenetic insights from a nuclear genome sequence of the extinct, giant, "subfossil" koala lemur Megaladapis edwardsi.},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {118},
number = {26},
pages = {},
pmid = {34162703},
issn = {1091-6490},
abstract = {No endemic Madagascar animal with body mass >10 kg survived a relatively recent wave of extinction on the island. From morphological and isotopic analyses of skeletal "subfossil" remains we can reconstruct some of the biology and behavioral ecology of giant lemurs (primates; up to ∼160 kg) and other extraordinary Malagasy megafauna that survived into the past millennium. Yet, much about the evolutionary biology of these now-extinct species remains unknown, along with persistent phylogenetic uncertainty in some cases. Thankfully, despite the challenges of DNA preservation in tropical and subtropical environments, technical advances have enabled the recovery of ancient DNA from some Malagasy subfossil specimens. Here, we present a nuclear genome sequence (∼2× coverage) for one of the largest extinct lemurs, the koala lemur Megaladapis edwardsi (∼85 kg). To support the testing of key phylogenetic and evolutionary hypotheses, we also generated high-coverage nuclear genomes for two extant lemurs, Eulemur rufifrons and Lepilemur mustelinus, and we aligned these sequences with previously published genomes for three other extant lemurs and 47 nonlemur vertebrates. Our phylogenetic results confirm that Megaladapis is most closely related to the extant Lemuridae (typified in our analysis by E. rufifrons) to the exclusion of L. mustelinus, which contradicts morphology-based phylogenies. Our evolutionary analyses identified significant convergent evolution between M. edwardsi and an extant folivore (a colobine monkey) and an herbivore (horse) in genes encoding proteins that function in plant toxin biodegradation and nutrient absorption. These results suggest that koala lemurs were highly adapted to a leaf-based diet, which may also explain their convergent craniodental morphology with the small-bodied folivore Lepilemur.},
}

@article {pmid34146486,
year = {2021},
author = {Baleka, S and Herridge, VL and Catalano, G and Lister, AM and Dickinson, MR and Di Patti, C and Barlow, A and Penkman, KEH and Hofreiter, M and Paijmans, JLA},
title = {Estimating the dwarfing rate of an extinct Sicilian elephant.},
journal = {Current biology : CB},
volume = {31},
number = {16},
pages = {3606-3612.e7},
doi = {10.1016/j.cub.2021.05.037},
pmid = {34146486},
issn = {1879-0445},
support = {310763/ERC_/European Research Council/International ; },
abstract = {Evolution on islands, together with the often extreme phenotypic changes associated with it, has attracted much interest from evolutionary biologists. However, measuring the rate of change of phenotypic traits of extinct animals can be challenging, in part due to the incompleteness of the fossil record. Here, we use combined molecular and fossil evidence to define the minimum and maximum rate of dwarfing in an extinct Mediterranean dwarf elephant from Puntali Cave (Sicily).1 Despite the challenges associated with recovering ancient DNA from warm climates,2 we successfully retrieved a mitogenome from a sample with an estimated age between 175,500 and 50,000 years. Our results suggest that this specific Sicilian elephant lineage evolved from one of the largest terrestrial mammals that ever lived3 to an island species weighing less than 20% of its original mass with an estimated mass reduction between 0.74 and 200.95 kg and height reduction between 0.15 and 41.49 mm per generation. We show that combining ancient DNA with paleontological and geochronological evidence can constrain the timing of phenotypic changes with greater accuracy than could be achieved using any source of evidence in isolation.},
}

@article {pmid34142055,
year = {2021},
author = {Roca-Rada, X and Politis, G and Messineo, PG and Scheifler, N and Scabuzzo, C and González, M and Harkins, KM and Reich, D and Souilmi, Y and Teixeira, JC and Llamas, B and Fehren-Schmitz, L},
title = {Ancient mitochondrial genomes from the Argentinian Pampas inform the early peopling of the Southern Cone of South America.},
journal = {iScience},
volume = {24},
number = {6},
pages = {102553},
pmid = {34142055},
issn = {2589-0042},
abstract = {The Southern Cone of South America (SCSA) is a key region for investigations about the peopling of the Americas. However, little is known about the eastern sector, the Argentinian Pampas. We analyzed 18 mitochondrial genomes-7 of which are novel-from human skeletal remains from 3 Early to Late Holocene archaeological sites. The Pampas present a distinctive genetic makeup compared to other Middle to Late Holocene pre-Columbian SCSA populations. We also report the earliest individuals carrying SCSA-specific mitochondrial haplogroups D1j and D1g from Early and Middle Holocene, respectively. Using these deep calibration time points in Bayesian phylogenetic reconstructions, we suggest that the first settlers of the Pampas were part of a single and rapid dispersal ∼15,600 years ago. Finally, we propose that present-day genetic differences between the Pampas and the rest of the SCSA are due to founder effects, genetic drift, and a partial population replacement ∼9,000 years ago.},
}

@article {pmid34140364,
year = {2021},
author = {Gibbons, A},
title = {Genomes offer rare glimpse of Neanderthal family groups.},
journal = {Science (New York, N.Y.)},
volume = {372},
number = {6548},
pages = {1251-1252},
doi = {10.1126/science.372.6548.1251},
pmid = {34140364},
issn = {1095-9203},
mesh = {Animals ; DNA, Ancient/*analysis ; DNA, Mitochondrial/*analysis ; Family ; Female ; *Genome ; Male ; Neanderthals/*genetics ; Sociological Factors ; Y Chromosome ; },
}

Animals
DNA, Ancient/*analysis
DNA, Mitochondrial/*analysis
Family
Female
*Genome
Male
Neanderthals/*genetics
Sociological Factors
Y Chromosome

@article {pmid34135378,
year = {2021},
author = {Smith, AD and Kamiński, MJ and Kanda, K and Sweet, AD and Betancourt, JL and Holmgren, CA and Hempel, E and Alberti, F and Hofreiter, M},
title = {Recovery and analysis of ancient beetle DNA from subfossil packrat middens using high-throughput sequencing.},
journal = {Scientific reports},
volume = {11},
number = {1},
pages = {12635},
pmid = {34135378},
issn = {2045-2322},
abstract = {The study of ancient DNA is revolutionizing our understanding of paleo-ecology and the evolutionary history of species. Insects are essential components in many ecosystems and constitute the most diverse group of animals. Yet they are largely neglected in ancient DNA studies. We report the results of the first targeted investigation of insect ancient DNA to positively identify subfossil insects to species, which includes the recovery of endogenous content from samples as old as ~ 34,355 ybp. Potential inhibitors currently limiting widespread research on insect ancient DNA are discussed, including the lack of closely related genomic reference sequences (decreased mapping efficiency) and the need for more extensive collaborations with insect taxonomists. The advantages of insect-based studies are also highlighted, especially in the context of understanding past climate change. In this regard, insect remains from ancient packrat middens are a rich and largely uninvestigated resource for exploring paleo-ecology and species dynamics over time.},
}

@article {pmid34129037,
year = {2021},
author = {Speidel, L and Cassidy, L and Davies, RW and Hellenthal, G and Skoglund, P and Myers, SR},
title = {Inferring Population Histories for Ancient Genomes Using Genome-Wide Genealogies.},
journal = {Molecular biology and evolution},
volume = {38},
number = {9},
pages = {3497-3511},
doi = {10.1093/molbev/msab174},
pmid = {34129037},
issn = {1537-1719},
support = {/WT_/Wellcome Trust/United Kingdom ; FC001595/ARC_/Arthritis Research UK/United Kingdom ; 220457/Z/20/Z//Sir Henry Wellcome fellowship/ ; 200186/Z/15/Z/WT_/Wellcome Trust/United Kingdom ; 212284/Z/18/Z//Wellcome Trust Investigator Award/ ; FC001595/WT_/Wellcome Trust/United Kingdom ; /CRUK_/Cancer Research UK/United Kingdom ; //UK Medical Research Council/ ; /WT_/Wellcome Trust/United Kingdom ; 852558/ERC_/European Research Council/International ; 217223/Z/19/Z//Wellcome Trust Investigator award/ ; //Vallee Foundation/ ; },
abstract = {Ancient genomes anchor genealogies in directly observed historical genetic variation and contextualize ancestral lineages with archaeological insights into their geography and cultural associations. However, the majority of ancient genomes are of lower coverage and cannot be directly built into genealogies. Here, we present a fast and scalable method, Colate, the first approach for inferring ancestral relationships through time between low-coverage genomes without requiring phasing or imputation. Our approach leverages sharing patterns of mutations dated using a genealogy to infer coalescence rates. For deeply sequenced ancient genomes, we additionally introduce an extension of the Relate algorithm for joint inference of genealogies incorporating such genomes. Application to 278 present-day and 430 ancient DNA samples of >0.5x mean coverage allows us to identify dynamic population structure and directional gene flow between early farmer and European hunter-gatherer groups. We further show that the previously reported, but still unexplained, increase in the TCC/TTC mutation rate, which is strongest in West Eurasia today, was already present at similar strength and widespread in the Late Glacial Period ~10k-15k years ago, but is not observed in samples >30k years old. It is strongest in Neolithic farmers, and highly correlated with recent coalescence rates between other genomes and a 10,000-year-old Anatolian hunter-gatherer. This suggests gene-flow among ancient peoples postdating the last glacial maximum as widespread and localizes the driver of this mutational signal in both time and geography in that region. Our approach should be widely applicable in future for addressing other evolutionary questions, and in other species.},
}

@article {pmid34128784,
year = {2021},
author = {Dorman, MJ and Thomson, NR and Campos, J},
title = {Genomic contextualisation of ancient DNA molecular data from an Argentinian fifth pandemic Vibrio cholerae infection.},
journal = {Microbial genomics},
volume = {7},
number = {6},
pages = {},
pmid = {34128784},
issn = {2057-5858},
support = {/WT_/Wellcome Trust/United Kingdom ; 206194/WT_/Wellcome Trust/United Kingdom ; },
}