@article {pmid41202131,
year = {2025},
author = {Liu, Y and Ban, Y and Gao, D},
title = {Oligo-CALL: A next-generation barcoding platform for studying resistance to targeted therapy.},
journal = {Science advances},
volume = {11},
number = {45},
pages = {eadw9990},
doi = {10.1126/sciadv.adw9990},
pmid = {41202131},
issn = {2375-2548},
mesh = {Humans ; *Drug Resistance, Neoplasm/genetics ; Cell Line, Tumor ; CRISPR-Cas Systems ; *Lung Neoplasms/genetics/drug therapy/pathology ; Single-Cell Analysis ; Molecular Targeted Therapy ; Proto-Oncogene Proteins p21(ras)/genetics/antagonists & inhibitors ; },
abstract = {Understanding therapy resistance requires deconvolving heterogeneous cell populations and tracking clonal trajectories. While CRISPR-based cellular barcoding is powerful for lineage tracing, many platforms suffer from low efficiency and limited compatibility with single-cell transcriptomics. We developed Oligo-CALL (Oligonucleotide-inducible CRISPR transcriptional activator-Assisted Lineage Labeling), an advanced barcoding system enabling precise lineage tracing, live clone isolation, and seamless integration with single-cell RNA sequencing. Applied to lung cancer cells treated with a KRAS[G12C] inhibitor, Oligo-CALL identified clones consistently enriched posttreatment, supporting a model of predestined resistance. Oligo-CALL achieved >95% efficiency in linking lineage identity to transcriptomes, uncovering diverse clone-specific pathways with underlying resistance. Paired analysis of barcode-matched clones from naïve and resistant populations revealed transient and fixed resistance phenotypes. Notably, DNA repair pathways are recurrently altered in resistant clones, and inhibition of poly(adenosine 5'-diphosphate-ribose) polymerase synergizes with KRAS G12C inhibition to overcome resistance. Together, Oligo-CALL provides a versatile platform for dissecting lineage evolution and molecular dynamics of targeted therapy resistance.},
}

Humans
*Drug Resistance, Neoplasm/genetics
Cell Line, Tumor
CRISPR-Cas Systems
*Lung Neoplasms/genetics/drug therapy/pathology
Single-Cell Analysis
Molecular Targeted Therapy
Proto-Oncogene Proteins p21(ras)/genetics/antagonists & inhibitors

@article {pmid41198699,
year = {2025},
author = {Lin, R and Xu, Y and Yang, S and Chen, S and Wang, Z and Wang, K and Gao, Z and Zhao, YS},
title = {Regioselective on-surface crystallization of one-dimensional organic barcode-like heterostructures of MOFs with fluorescent stripe patterns.},
journal = {Nature communications},
volume = {16},
number = {1},
pages = {9810},
pmid = {41198699},
issn = {2041-1723},
support = {2022YFA1204403//Ministry of Science and Technology of the People's Republic of China (Chinese Ministry of Science and Technology)/ ; },
abstract = {Heterostructures, integrating different semiconducting materials in a single structure, are key components for various modern electrical and optoelectronic devices. However, it is difficult to maintain the sequential epitaxial nucleation due to the passivation of crystal seeds, thus resulting in limited spatial segments in heterostructures. Here, we report an interface-recognition assembly strategy assisted by Plateau-Rayleigh instability (PRI) to spontaneously form one-dimensional (1D) heterostructures featuring abundant fluorescent stripe patterns. Guided by 1D metal-organic frameworks (MOFs) templates with exposed electron-donating sites, the de-wetting process promotes the effective regioselective crystallization of solute molecules with electron-withdrawing feature on template surface, thus forming 1D barcode-like heterostructures. The spatial locations and number of the stripes are effectively controlled by PRI and template parameters, while their emission colors are primarily governed by the charge-transfer (CT) interactions between solutes. On this basis, we have achieved full-color and heterogeneous stripe patterns by tuning the CT strengths and controlling the stripe nucleation sequences, thus creating abundant 1D barcode-like heterostructures. Through integrating the photoisomers into stripes, these heterostructures exhibit time-dependent graphical patterns and enable construction of 2D photonic barcodes. Our work offers a supramolecular approach for patterning of well-aligned organic heterostructures and rational design of optoelectronic devices that have potential in anti-counterfeiting applications.},
}

@article {pmid41193635,
year = {2025},
author = {Weinheimer, AR and Brown, JM and Thompson, B and Leonaviciene, G and Kiseliovas, V and Jocys, S and Munson-McGee, J and Gavelis, G and Mascena, C and Mazutis, L and Poulton, NJ and Zilionis, R and Stepanauskas, R},
title = {Single-particle genomics uncovers abundant non-canonical marine viruses from nanolitre volumes.},
journal = {Nature microbiology},
volume = {},
number = {},
pages = {},
pmid = {41193635},
issn = {2058-5276},
support = {991222//Simons Foundation/ ; },
abstract = {Viruses and other extracellular genetic elements play essential roles in marine communities. However, methods to capture their full diversity remain limited by the constraints of bulk sequencing assemblers or pre-sorting throughput. Here we introduce environmental micro-compartment genomics (EMCG), which vastly improves the throughput and efficiency of single-particle genomic sequencing obtained from nanolitre volumes by compartmentalizing particles of a sample into picolitre-sized, semi-permeable capsules for in-capsule DNA amplification and barcoding. From 300 nanolitres of seawater, EMCG obtained genomic sequences of 2,037 particles. The microbiome composition agreed with other methods, and the virus-like assembly lengths indicated that most were near complete. Many viral assemblies belonged to the Naomiviridae, lacked metagenomic representation and aligned to outlier contigs of abundant, putative host lineages, suggesting their use of non-canonical DNA and overlooked ecological importance. This approach provides opportunities for high-throughput, quantitative and cost-effective genome analyses of individual cells and extracellular particles across complex microbiomes.},
}

@article {pmid41193241,
year = {2025},
author = {Kawabata, H and Miura, O},
title = {Excess of non-synonymous mutations and structural variations in mitogenomes of freshwater snails in the genus Semisulcospira.},
journal = {Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis},
volume = {},
number = {},
pages = {1-8},
doi = {10.1080/24701394.2025.2583076},
pmid = {41193241},
issn = {2470-1408},
abstract = {Mitochondrial DNA has unique biological characteristics, such as a high mutation rate and clonal inheritance, which make it an appropriate marker in molecular biology. However, exceptionally high mitochondrial divergences have been reported in terrestrial and marine invertebrates, plants, and certain vertebrate lineages, which complicates the interpretation of the results in DNA barcoding and evolutionary studies. The freshwater snails in the genus Semisulcospira have two mitochondrial DNA lineages: a normal mitochondrial lineage (mt-A type) and a highly divergent 'enigmatic' lineage (mt-B type). We assembled thirty-two mitogenomes of Semisulcospira to understand the molecular evolution of the mt-B type. We found that the mt-B type evolves faster than the mt-A type, has more non-synonymous mutations, and exhibits structural variations with truncated cob and cox3 genes. Our results showed that the observed molecular evolution of the mt-B type could result from a decreased efficiency of natural selection due to small population size and/or the accumulation of slightly deleterious mutations caused by mitochondrial sex ratio distortion.},
}

@article {pmid41193180,
year = {2025},
author = {Hormiga, G and Lavery, AH and Arnedo, MA},
title = {The spider genus Stictonanus Millidge, 1991 in the Juan Fernandez Archipelago (Araneae, Linyphiidae): systematics and biogeography.},
journal = {Invertebrate systematics},
volume = {39},
number = {11},
pages = {},
doi = {10.1071/IS25046},
pmid = {41193180},
issn = {1447-2600},
mesh = {Animals ; *Spiders/classification/anatomy & histology/genetics ; *Phylogeny ; Phylogeography ; Male ; },
abstract = {We studied the systematics of the Juan Fernandez linyphiid species originally classified in the genus Juanfernandezia Koçak & Kemal, 2008. In an earlier study, we had hypothesized that the two Juanfernandezia species, each endemic to a single island, form a clade that was sister to the southern South American genus Notholepthyphantes Millidge, 1985. We have carried out a multi-locus phylogenetic analysis to infer the relationships of Juanfernandezia based on an extended taxon sampling. Our study shows that Juanfernandezia is sister to the South American genus Stictonanus Millidge, 1991, which until recently remained poorly known. The striking similarity of the somatic and genitalic morphology of these two genera suggest that the clade is better classified as a single genus, with Stictonanus being the oldest available generic name for this lineage. Stictonanus, circumscribed now to include five species, is sister to a lineage composed of two small genera, Notholepthyphantes and Falklandoglenes Usher, 1986. Stictonanus fernandezi (Berland, 1924) comb. nov. and Stictonanus paolae Hormiga & Arnedo, sp. nov., both from the Juan Fernandez Islands, are described and illustrated. The type species of the genus, Stictonanus paucus Millidge, 1991, and the second continental species, S. exiguus Millidge, 1991, are here redescribed and illustrated (the male of S. exiguus is described here for the first time). We further infer a time calibrated tree to discuss the biogeographic history of Stictonanus. ZooBank: urn:lsid:zoobank.org:pub:C6D833D0-0422-401C-B010-B12618B1633C.},
}

Animals
*Spiders/classification/anatomy & histology/genetics
*Phylogeny
Phylogeography
Male

@article {pmid41192368,
year = {2025},
author = {Lin, YT and Lin, YH and Shiu, YW and Han, YS},
title = {Morphological and molecular evidence reveal the Sarotherodon melanotheron invasion off Taiwan, East Asia.},
journal = {Marine pollution bulletin},
volume = {222},
number = {Pt 3},
pages = {118898},
doi = {10.1016/j.marpolbul.2025.118898},
pmid = {41192368},
issn = {1879-3363},
abstract = {The blackchin tilapia, Sarotherodon melanotheron Rüppell, 1852, is a highly euryhaline cichlid native to West and Central Africa. Due to its broad salinity tolerance and rapid reproduction, it has become a high-risk invasive species in brackish and coastal ecosystems worldwide. This study reports the first and northernmost confirmed record of wild blackchin tilapia population in East Asia, based on 13 specimens collected from coastal waters of Kaohsiung City, Southern Taiwan. Morphological and meristic data, together with cytochrome c oxidase subunit I (COI) gene sequencing, confirmed species identity and consistency with native and invasive populations elsewhere. The occurrence of mature individuals and a substantial wild population confirms the establishment of invasion population in Taiwanese coastal environment. Given its wide salinity tolerance and omnivorous diet, this species poses potential ecological and economic threats to native ecosystems and aquaculture. This finding also represents the northernmost record of blackchin tilapia in the western Pacific.},
}

@article {pmid41187829,
year = {2025},
author = {Simonson, AW and Chao, MC and Hood, LE and Donlan, RA and Hopkins, F and Chase, MR and Vickers, AJ and Callendrello, A and Klein, E and Borish, H and Malin, M and Maiello, P and Scanga, CA and Lin, PL and Fortune, SM and Flynn, JL},
title = {Protection against reinfection with Mycobacterium tuberculosis extends across heterologous Mtb lineages.},
journal = {Mucosal immunology},
volume = {},
number = {},
pages = {},
doi = {10.1016/j.mucimm.2025.10.010},
pmid = {41187829},
issn = {1935-3456},
abstract = {Immunological memory elicited either through previous or ongoing M. tuberculosis (Mtb) infection provides a critical mechanism by which hosts protect against re-infection and disease progression upon Mtb re-exposure. Conversely, the uneven competition between distinct Mtb strains suggest certain bacterial clades have enhanced ability to spread across communities and circulate globally, potentially by evading memory responses gained by prior infection with genomically different strains. To address whether memory responses induced by one strain can protect against a genetically distinct strain, we conducted a heterologous reinfection study in cynomolgus macaques involving primary infection by a Lineage 4 Erdman Mtb strain and subsequent re-challenge by a Lineage 2 strain, HT-L2. Recent epidemiologic studies have shown that the clade to which HT-L2 belongs has been spreading successfully over the last decade in Lima, Peru. Here, through microbiologic, PET-CT imaging and sequencing of Mtb genomic barcodes, we show that reinfected animals developed fewer lung lesions and controlled both pulmonary and disseminated forms of infection better than naïve animals without prior exposure to Mtb. Our data support that protection against reinfection is not limited by Mtb lineage, providing optimism that vaccines can be effective across populations and geographic locations.},
}

@article {pmid41187186,
year = {2025},
author = {Altaha, S and Al-Qaoud, K and Al-Omari, M and Al-Shawaheen, A},
title = {Development of multiplex immuno-PCR diagnostic platform using chicken IgY antibodies for COVID-19 diagnosis.},
journal = {Journal of infection in developing countries},
volume = {19},
number = {10},
pages = {1455-1463},
doi = {10.3855/jidc.21414},
pmid = {41187186},
issn = {1972-2680},
mesh = {Animals ; Chickens/immunology ; *Immunoglobulins/immunology ; *COVID-19/diagnosis ; *SARS-CoV-2/immunology/genetics ; *Multiplex Polymerase Chain Reaction/methods ; *Antibodies, Viral/immunology ; Sensitivity and Specificity ; Humans ; Enzyme-Linked Immunosorbent Assay ; *COVID-19 Serological Testing/methods ; Spike Glycoprotein, Coronavirus/immunology ; },
abstract = {INTRODUCTION: The coronavirus disease 2019 (COVID-19) pandemic has significantly accelerated the development of diagnostic techniques. Real‑time quantitative polymerase chain reaction (RT‑qPCR) was the method of choice for diagnosis and was considered as the gold standard. However, limited specificity of RT-PCR was noticed during the pandemic. This research aimed to develop a combined highly specific immune-based and highly sensitive molecular-based diagnostic technique.

METHODOLOGY: Groups of chicken were immunized with commercial severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) N, S, and E antigens. The IgY antibodies were purified from eggs using a High-Trap IgY affinity column. Three unique DNA barcodes were designed, synthesized, and amplified using 5'-amine-labeled forward primers. DNA barcodes purified form PCR products were coupled to IgY antibodies using the (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide) - N-hydroxysuccinimide (EDC-NHS) coupling chemistry. ELISA; SDS-PAGEs; immunoblot (IB); and uniplex-, duplex- and multiplex immuno-PCR (IPCR) were used to confirm system validity.

RESULTS: Amplification of single barcodes using RT-PCR showed a Ct value of 15, with no significant variation when amplified in duplex or multiplex formats. Chicken IgY-DNA barcode conjugation and reactivity were verified using IB and ELISA. IPCR resulted in efficient amplification of all three DNA barcodes in uniplex, duplex, and multiplex formats after binding to commercial N, S, and E antigens.

CONCLUSIONS: The successful combination of the specific antibody-based techniques, low-cost chicken IgY antibodies, and RT-PCR sensitivity achieved in this study present a promising approach to meet the demand for sensitive and accurate diagnostics. This generic platform can be adopted in any analyte detection system.},
}

Animals
Chickens/immunology
*Immunoglobulins/immunology
*COVID-19/diagnosis
*SARS-CoV-2/immunology/genetics
*Multiplex Polymerase Chain Reaction/methods
*Antibodies, Viral/immunology
Sensitivity and Specificity
Humans
Enzyme-Linked Immunosorbent Assay
*COVID-19 Serological Testing/methods
Spike Glycoprotein, Coronavirus/immunology

@article {pmid41184589,
year = {2025},
author = {Bernadskaya, YY and Kuan, A and Tjärnberg, A and Brandenburg, J and Zhang, P and Wiechecki, K and Kaplan, N and Failla, M and Bikou, M and Madilian, O and Bruderer, N and Wang, W and Christiaen, L},
title = {Cell cycle-driven transcriptome maturation confers multilineage competence to cardiopharyngeal progenitors.},
journal = {The EMBO journal},
volume = {},
number = {},
pages = {},
pmid = {41184589},
issn = {1460-2075},
support = {HL108643//HHS | NIH | NHLBI | Division of Intramural Research (DIR)/ ; HD096770//HHS | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)/ ; 32270870//National Natural Science Foundation of China/ ; },
abstract = {During development, stem and progenitor cells divide and transition through multipotent states to generate the diverse cell types by undergoing defined changes in biomolecular composition, which underlie the progressive loss of potency and acquisition of lineage-specific characteristics. For example, the cardiac and pharyngeal muscle programs are jointly primed in multipotent cardiopharyngeal progenitors, and segregate in distinct daughter cells only after cell division. Here, using the tunicate Ciona, we showed that multipotent cardiopharyngeal progenitors acquire the competence to produce distinct Tbx1/10 (+) and (-) daughter cells shortly before mitosis, which is necessary for Tbx1/10 activation. By combining transgene-based sample barcoding with single-cell RNA-sequencing (scRNA-seq), we uncovered transcriptome-wide dynamics in migrating cardiopharyngeal progenitors as cells progress through G1, S, and G2 phases. We refer to this process as "transcriptome maturation", and identified candidate mature genes, including the Rho GAP-coding gene Depdc1b, which peaks in late G2. Functional assays indicated that transcriptome maturation fosters cardiopharyngeal competence, in part through multilineage priming and by enabling asymmetric cell division that influences subsequent fate decisions, illustrating the concept of "behavioral competence". We show that both classic regulatory circuits and coupling with the G1-S transition drive transcriptome maturation, ensuring the timely deployment of lineage-specific programs.},
}

@article {pmid41183194,
year = {2025},
author = {Gentry, K and Lian, L and Kim, H and Celik, O and Jones, C and Podilapu, AR and Shakked, A and Loughrey, D and Zenhausern, R and Jang, B and Doan, J and Rudden, S and Dahlman, JE},
title = {Glycolipid nanoparticles target the spleen and detarget the liver without charge.},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {122},
number = {45},
pages = {e2409569122},
doi = {10.1073/pnas.2409569122},
pmid = {41183194},
issn = {1091-6490},
support = {Dahlman Lab//Amgen Inc. | Amgen Foundation (The Amgen Foundation)/ ; },
mesh = {*Liver/metabolism/drug effects ; *Spleen/metabolism/drug effects ; Animals ; *Glycolipids/chemistry ; *Nanoparticles/chemistry/administration & dosage ; Mice ; Humans ; Mice, Inbred C57BL ; },
abstract = {Lipid nanoparticles (LNPs) formulated with a neutral helper lipid can deliver RNA to the liver in humans. However, clinically relevant delivery to other tissues has remained challenging. To avoid the liver, scientists often add antibodies or helper lipids with a permanent charge. Here, we report an alternative approach: antibody- and charge-independent liver detargeting. Using DNA barcoding to test 109 chemically distinct LNPs in vivo, we found that replacing a neutral helper lipid with a neutral glycolipid reduced liver delivery and increased splenic delivery. Consistent with this differential tropism, these glycolipid nanoparticles caused differences in downstream cellular signaling in vivo compared to traditional LNPs. These data suggest that extrahepatic LNPs can be designed without the imposition of a net negative or positive charge.},
}

*Liver/metabolism/drug effects
*Spleen/metabolism/drug effects
Animals
*Glycolipids/chemistry
*Nanoparticles/chemistry/administration & dosage
Mice
Humans
Mice, Inbred C57BL

@article {pmid41181664,
year = {2025},
author = {Monckton, SK and Levesque-Beaudin, V and Thompson, KA and Barnes, A and French, K and Hampton, H and Hebert, PDN},
title = {The brown copper moth, Tridentaforma browncopper: DNA barcoding reveals a second species in the family Tridentaformidae (Lepidoptera, Adeloidea).},
journal = {ZooKeys},
volume = {1257},
number = {},
pages = {25-38},
pmid = {41181664},
issn = {1313-2989},
abstract = {A biodiversity monitoring program in south-central British Columbia, Canada, at sites on traditional nłeʔképmx territory near the Highland Valley Copper mine, collected many specimens of an unknown moth. DNA barcode analysis revealed its affinity but deep divergence (14.3%) from Tridentaforma fuscoleuca (Braun, 1923), the only species in the family Tridentaformidae. The new species was assigned to the BIN (Barcode Index Number) BOLD:AFK8960. Morphological study confirmed its placement in this family but revealed marked genitalic differences from T. fuscoleuca. Given its genetic and morphological divergence, we describe Tridentaforma browncopper Monckton & Levesque-Beaudin, sp. nov. The scientific name is a translation of the name, skʷúnkʷl̓itkax̣n̓I, chosen by nłeʔképmx Elders and Knowledge Keepers, which means "brown copper moth". Its discovery and naming reflect an effective collaboration among biodiversity scientists, industry representatives, and Indigenous communities. It also demonstrates how DNA barcoding can facilitate species descriptions without requiring taxonomists with specialist expertise in the group under investigation.},
}

@article {pmid41179342,
year = {2025},
author = {Bang, WJ and You, JY and Bae, Y and Chuang, MF and Shin, S},
title = {Preliminary Study on Host Use and Phylogenetic Analysis of Corethrella nippon in Taiwan.},
journal = {Ecology and evolution},
volume = {15},
number = {11},
pages = {e72405},
pmid = {41179342},
issn = {2045-7758},
abstract = {This study investigated frog-biting dipteran species using newly designed frog-calling traps in Taiwan. The trap effectively collected specimens from both families, Culicidae and Corethrellidae, demonstrating its utility. Host preference analysis revealed that Odorrana swinhoana (Boulenger, 1903) and Kurixalus eiffingeri (Boettger, 1895) were most frequently associated with collected specimens of Corethrellidae. Additionally, the corethrellids were predominantly attracted to a sound frequency around 2200 to 2700 Hz. Then, DNA barcoding was also conducted on the four collected species of Culicidae: Armigeres subalbatus (Coquillett, 1898), Uranotaenia nivipleura Leicester, 1908, Ur. macferlanei Edwards, 1914, and Mimomyia luzonensis (Ludlow, 1905), and the mitochondrial genome of Corethrella nippon Miyagi 1980 was first sequenced and annotated. Mitogenome-based phylogenetic analysis confirmed that C. nippon formed a clade with Corethrella condita Borkent, 2008. In our analysis, family Corethrellidae clustered with Culicidae; however, the inter-family phylogenetic relationships within Culicoidea appeared paraphyletic, particularly concerning family Chaoboridae. Future studies should explore a greater variety of frog species across more diverse regions and use other genomic datasets beyond the mitogenome to infer a more robust deep topology at the superfamily level and further broaden our understanding of host preference.},
}

@article {pmid41179240,
year = {2025},
author = {Harefa, T and Haryono, H and Gustiano, R and Sukmono, T and Wahyudewantoro, G},
title = {Desmopuntius mahakamensis, a new cyprinid species (Teleostei, Cyprinidae) from East Kalimantan, Indonesia.},
journal = {ZooKeys},
volume = {1256},
number = {},
pages = {371-391},
pmid = {41179240},
issn = {1313-2989},
abstract = {Desmopuntius mahakamensis sp. nov., is described based on specimens from East Kalimantan, Indonesia. The new species, D. mahakamensis is distinguished from all congeners by the following combination of characters: anal-fin rays iii,5½; lateral line scales 26-27 (mode 26); predorsal scales 9-11 (mode 10); gill rakers 9-11 (mode 10); total vertebrae 29; axial streak present; presence of 5-6 black lateral stripes in adults (>30 mm SL), with stripe +1 extending from behind the gill opening along scale row 1 and reaching only to midbody, between the origin and end of the dorsal-fin base. Molecular evidence further supports the assignment of the new species in the genus Desmopuntius and its clear separation from congeners.},
}

@article {pmid41176652,
year = {2025},
author = {Yang, X and Gao, Z},
title = {Regulation of Supramolecular Interactions: RNA:DNA Folding and Nanopore Sensing Applications.},
journal = {Chembiochem : a European journal of chemical biology},
volume = {},
number = {},
pages = {e202500709},
doi = {10.1002/cbic.202500709},
pmid = {41176652},
issn = {1439-7633},
support = {22176080//National Natural Science Foundation of China/ ; ZR2023YQ015//Natural Science Foundation of Shandong Province/ ; },
abstract = {The intersection of DNA/RNA nanotechnology and single-molecule solid-state nanopore sensing enables precise detection of low-abundance biomolecules. However, a significant challenge persists that nucleic acid molecules readily fold during nanopore translocation, which gives rise to ambiguous current signatures, reduces the number of usable data points, and compromises detection accuracy. Herein, a recent advancement by the Platnich group is highlighted, wherein urea is employed as a noncovalent modulator to participate in the isothermal assembly of MS2 bacteriophage RNA with DNA barcodes. This modulator stably binds to the RNA:DNA hybrids through supramolecular interactions after removal of free urea by buffer exchange. Nanopore assays showed a ≈50% reduction in fold-shift events in the urea-treated group. Atomic force microscopy characterization shows that the persistence length of the hybrids is enhanced by ≈40%. This strategy provides a new noncovalent regulatory tool for nucleic acid nanotechnology and pushes the nanopore sensing technology toward the precision detection of low-abundance RNA.},
}

@article {pmid41174477,
year = {2025},
author = {Ren, T and Xun, L and Jia, Y and Zhou, H and Li, B},
title = {Insights into the plastome evolution and phylogenetic relationships of Lepidium (Brassicaceae).},
journal = {BMC plant biology},
volume = {25},
number = {1},
pages = {1489},
pmid = {41174477},
issn = {1471-2229},
support = {2023K-25//Science and Technology Program of Shaanxi Academy of Science/ ; 2023K-14//Science and Technology Program of Shaanxi Academy of Science/ ; 2022K-04//Key Scientific Research Project of Shaanxi Academy of Sciences/ ; 32300314//National Natural Science Foundation of China/ ; [KRDL] K6-2207039//First Investigation of Wild Plants Resources in Xi'an/ ; },
mesh = {*Phylogeny ; *Lepidium/genetics/classification ; *Evolution, Molecular ; *Genome, Plastid/genetics ; *Plastids/genetics ; },
abstract = {BACKGROUND: Lepidium represents one of the most species-rich genera in the Brassicaceae, encompassing numerous taxa with excellent medicinal value. Although the plastome architecture of Brassicaceae has been extensively characterized, its evolutionary dynamics within Lepidium remain poorly understood. Besides, the intrageneric taxonomy has long been problematic, primarily due to subtle morphological differences or overlapping morphological characteristics. Here, 15 Lepidium plastomes (11 newly sequenced and 4 published) were subject to comparative genomic and phylogenetic analyses to investigate plastome evolution and phylogenetic relationships.

RESULTS: The total length of the 11 newly sequenced Lepidium plastomes varied from 153,803 bp to 154,997 bp, with 111-112 unique genes. The Lepidium plastomes exhibited strong conservatism in genome size, gene order, plastome structure, IR/SC borders, codon usage, mVISTA analysis, Pi value, and genetic distance. However, minor variations were observed in IR border shifts. Analysis of repeat sequences revealed interspecific differences in both long repeat sequences (24-40) and simple sequence repeats (78-98) among 15 Lepidium plastomes. Six divergence hotspots (ycf1, ccsA, matK, rps15-ycf1, trnH-GUG-psbA, and rpl32-trnL-UAG) were identified as potential DNA barcodes for both species identification and phylogenetic analyses of Lepidium. The ycf1 gene was under relaxed selection. Furthermore, our phylogenetic studies clarified that all 15 Lepidium species formed a strongly supported monophyletic group within the tribe Lepidieae, while the cytonuclear discordance between ITS and plastome trees was found within Lepidium.

CONCLUSION: Our findings suggested that cytonuclear discordance within Lepidium likely results from hybridization/introgression and incomplete lineage sorting. Meanwhile, we proposed the reclassification of L. perfoliatum within Section Lepia. This study clearly documented global patterns of plastome structural evolution, and constructed a well-supported phylogeny of Lepidium. The reliable phylogenetic tree not only provides a new viewpoint into the intrageneric relationships and sectional classification of Lepidium, but also advances our understanding of the evolutionary history of this genus.},
}

*Phylogeny
*Lepidium/genetics/classification
*Evolution, Molecular
*Genome, Plastid/genetics
*Plastids/genetics

@article {pmid41173855,
year = {2025},
author = {Dahlberg, SK and Bonet, DF and Franzén, L and Ståhl, PL and Hoffecker, IT},
title = {Hidden network preserved in Slide-tags data allows reference-free spatial reconstruction.},
journal = {Nature communications},
volume = {16},
number = {1},
pages = {9652},
pmid = {41173855},
issn = {2041-1723},
support = {949624//EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)/ ; 2020-05368//Vetenskapsrådet (Swedish Research Council)/ ; },
mesh = {*Gene Expression Profiling/methods ; *Oligonucleotide Array Sequence Analysis/methods ; Humans ; Animals ; Mice ; Transcriptome ; },
abstract = {Spatial transcriptomics technologies aim to spatially map gene expression in tissues and typically use oligonucleotide array surfaces that have undergone spatial indexing. These arrays are used to capture nucleic acids diffusing from adjacently placed tissues, allowing subsequent sequencing to reveal both gene and position. Slide-tags is a recently developed method by Russell et al. that inverts this principle. Instead of capturing molecules released from the tissue, probes are detached from a pre-decoded bead array and diffused into tissues, tagging nuclei with spatial barcodes. In this work we reanalyze this data and discover a latent, spatially informative cell-bead network formed incidentally from barcode diffusion and the biophysical properties of the tissue. This allows us to treat Slide-tags as a network-based imaging-by-sequencing approach. By optimizing spatial constraints encoded in the cell-bead network structure, we can achieve unassisted tissue reconstruction, a fundamental shift from classical spatial technologies based on pre-indexed arrays.},
}

*Gene Expression Profiling/methods
*Oligonucleotide Array Sequence Analysis/methods
Humans
Animals
Mice
Transcriptome

@article {pmid41172905,
year = {2025},
author = {Huang, X and Lu, L and Yan, Y and Wu, L and Qi, H and Li, M and Lin, Y and Zhou, Q and Lu, Y},
title = {DNA self-assembly-based direct fluorescence encoding of targets enables simultaneous 10-plex microRNA detection.},
journal = {Biosensors & bioelectronics},
volume = {293},
number = {},
pages = {118130},
doi = {10.1016/j.bios.2025.118130},
pmid = {41172905},
issn = {1873-4235},
abstract = {As crucial post-transcriptional regulators, microRNAs (miRNAs) serve as valuable biomarkers for disease diagnosis and precision medicine. Current detection technologies (e.g., Luminex xMAP), however, suffer from technical limitations including elevated coefficient of variation and suboptimal sensitivity. To address these challenges, we developed an innovative imaging-based detection system leveraging DNA self-assembly and fluorescence barcoding technologies. Our system demonstrates two breakthrough features: (1) Programmable DNA assemblies facilitate highly specific target recognition with built-in signal amplification, achieving enhanced sensitivity through magnetic bead-mediated target enrichment; and (2) A rationally designed four-color fluorescent encoding system can generate 33 unique barcode combinations, from which we select 10 barcode combinations that allow multiplexed detection of 10 miRNAs in a single-tube reaction vessel. Validation studies using breast cancer cell lines (MCF-7) confirmed the system's robust performance in complex biological matrices. Our platform offers distinct advantages of streamlined workflow, cost-effectiveness and superior reproducibility, and is expected to provide a new technical means for early tumor screening and precision medicine.},
}

@article {pmid41171823,
year = {2025},
author = {Khudamrongsawat, J and Krajangdara, T and Panithanarak, T and Karuwancharoen, R and Klangnurak, W and Promnun, P and Senanan, W},
title = {DNA barcoding for elasmobranch diversity assessment in Thailand: Its advantages and limitations.},
journal = {PloS one},
volume = {20},
number = {10},
pages = {e0334640},
pmid = {41171823},
issn = {1932-6203},
mesh = {Animals ; *DNA Barcoding, Taxonomic/methods ; Thailand ; *Biodiversity ; *Elasmobranchii/genetics/classification ; Phylogeny ; Genetic Variation ; DNA, Mitochondrial/genetics ; Species Specificity ; },
abstract = {The assessment of elasmobranch biodiversity in Thailand benefits greatly from the application of DNA barcoding, which helps mitigate the challenge posed by a shortage of expert taxonomists. Fragments of COI and ND2 mitochondrial DNA were examined, and the strengths and weaknesses of these two markers were compared. In this study, DNA products from 153 elasmobranch samples were amplifiable and revealed a total of 28 shark species and 32 batoid species. Many species could be confidently identified as their morphological characteristics aligned with DNA barcodes. However, several exceptions were recognized. The absence of reference sequences for rare species presented a challenge for species verification, and the misidentification of reference sequences, as well as changes in species names due to taxonomic revisions, added complexity when comparing DNA barcoding sequences. Conflicts between morphology and genetics were also observed. While intraspecific genetic variation based on both DNA barcodes generally indicated 0-2% variation, this metric could not always be used for species delimitation. This was particularly true for species displaying low genetic variation among closely related species and species where cryptic diversity remained hidden and yet to be uncovered. In such cases, the morphological characteristics of the samples served as the primary means of species identification. Despite these challenges, DNA barcoding remains an invaluable tool for biodiversity assessment, especially in light of the shortage of skilled experts, and for identification of products made from vulnerable species. However, it is essential to exercise caution and be aware of these complexities in its application.},
}

Animals
*DNA Barcoding, Taxonomic/methods
Thailand
*Biodiversity
*Elasmobranchii/genetics/classification
Phylogeny
Genetic Variation
DNA, Mitochondrial/genetics
Species Specificity

@article {pmid41169085,
year = {2025},
author = {Ferreira da Silva, MJ and Colmonero-Costeira, I and Djaló, SL and Camará, T and Sá, RM and Minhós, T and Kiebler, A and Grethlein, M and Pikkarainen, N and Prost, S},
title = {Using miniaturized laboratory equipment and DNA barcoding to improve conservation genetics training and identify illegally traded species.},
journal = {Conservation biology : the journal of the Society for Conservation Biology},
volume = {},
number = {},
pages = {e70165},
doi = {10.1111/cobi.70165},
pmid = {41169085},
issn = {1523-1739},
support = {232533027//Mohamed bin Zayed Species Conservation Fund/ ; NORTE-01-0145-FEDER-000046//European Regional Development Fund/ ; //Genetic Society/ ; PCI#1694//Primate Conservation Incorporated/ ; Biodiverse Anthropocenes research//Academy of Finland PROFI6 336449/ ; PRIMACTION//Born Free Foundation/ ; UID/00713/2020//Fundação para a Ciência e a Tecnologia/ ; },
abstract = {Illegal wildlife trade (IWT) is one of the largest global illegal activities, and it negatively affects biodiversity and sustainable development worldwide. DNA barcoding coupled with high-throughput sequencing (i.e., metabarcoding) is useful in identifying taxa affected by IWT and has been used routinely for decades. However, for countries lacking laboratory infrastructure, sequencing units, and trained staff, the application of DNA barcoding tools in conservation is limited and depends on slow sample transport processes and molecular analyses carried out abroad. Guinea-Bissau, on the West African coast, has one of the lowest human development indices in the world and is a biodiversity hotspot threatened by IWT. We explored the potential use of inexpensive and portable miniaturized laboratory equipment (MLE) and DNA barcoding tools to improve training in conservation genetics and identification of traded species. We tested these technologies in tissue samples collected at different times and contexts in Guinea-Bissau and used 3 primer pairs amplifying mitochondrial DNA fragments. We successfully identified 33 tissue samples to the species level; thus, MLE may accelerate the use of DNA and metabarcoding methods in countries that have low research funding and limited infrastructure. The use of these technologies has the potential to advance the discipline of conservation genetics in Guinea-Bissau and other countries and to train students and employees of government agencies dedicated to investigating environmental crimes.},
}

@article {pmid41168956,
year = {2025},
author = {Ruiz, E and Lamy, T and Mouillot, D and Durand, JD},
title = {Benchmarking the Taxonomic Resolution of Fish eDNA Metabarcodes Against COI Barcodes.},
journal = {Molecular ecology resources},
volume = {},
number = {},
pages = {e70069},
doi = {10.1111/1755-0998.70069},
pmid = {41168956},
issn = {1755-0998},
abstract = {Even though environmental DNA metabarcoding is revolutionizing biomonitoring, many critical steps remain unstandardized, leading to arbitrary choices, particularly regarding the selection of metabarcode, clustering method and similarity threshold, among others. Additionally, these studies were hindered by biases resulting from the presence of mislabeled sequences in international databases such as GenBank and the lack of explicit definitions for taxonomic resolution. To address these issues, we developed a robust framework to compare the performance of 22 metabarcodes derived from the same mitogenomes (all available for Actinopterygians in NCBI) against a standardized taxonomic baseline based on COI Barcode Index Numbers (BINs). This framework allows for the separate quantification of over-splitting (splitting the same taxon/BIN) and over-merging (merging different taxon/BIN). Comparison of OTUs obtained with multiple de novo clustering methods to BINs confirmed the metabarcode ranking based on error sums. Although each metabarcode exhibited varying sensitivities to over-merging or over-splitting errors, the clustering threshold emerged as the most important factor influencing biodiversity estimates whatever the clustering method. This led us to propose optimal thresholds for each metabarcode to delineate taxonomic levels (metabarcode gaps). Additionally, we found that taxonomic resolution varied significantly among genes, orders and community diversity, but independently of metabarcode length. Overall, the choice of metabarcode and clustering threshold should aim to minimize over-merging or over-splitting while ensuring accurate lower taxonomic delineations. A set of documented R functions makes this evaluation of taxonomic resolution easily applicable to any other taxonomic group for which a representative set of full genes or mitogenomes is available.},
}

@article {pmid41168495,
year = {2025},
author = {Chang, T and Zhao, S and Deng, K and Liao, Z and Tang, M and Zhu, Y and Han, W and Yu, C and Fan, W and Jiang, M and Wang, G and Liu, D and Peng, J and Pang, Y and Fei, P and Wang, J and Zheng, C and Huang, Y},
title = {High-plex spatial RNA imaging in one round with conventional microscopes using color-intensity barcodes.},
journal = {Nature biotechnology},
volume = {},
number = {},
pages = {},
pmid = {41168495},
issn = {1546-1696},
support = {T2188102//National Natural Science Foundation of China (National Science Foundation of China)/ ; T2225005//National Natural Science Foundation of China (National Science Foundation of China)/ ; Z221100007022003//Beijing Municipal Science and Technology Commission/ ; },
abstract = {Spatial RNA imaging has not been widely adopted because conventional fluorescence microscopy is limited to only a few channels and the cyclic reactions needed to increase multiplexing in techniques such as sequential fluorescence in situ hybridization require sophisticated instrumentation. Here, we introduce 'profiling of RNA in situ through single-round imaging' (PRISM), a method that expands coding capacity through color intensity grading. Using a radius vector filtering strategy to ensure the distinguishability of codewords in color space, PRISM achieves up to 64-plex color-barcoded RNA imaging in a single imaging round with conventional microscopes. We validate PRISM's versatility across various tissues by generating a three-dimensional (3D) atlas of mouse embryonic development from E12.5 to E14.5, a quasi-3D tumor-normal transition landscape of human hepatocellular carcinoma and a 3D cell atlas and subcellular RNA localization landscapes of mouse brain. Additionally, we show the critical role of cancer-associated fibroblasts in mediating immune infiltration and immune response heterogeneity within and between tumor microenvironments.},
}

@article {pmid41168426,
year = {2025},
author = {Tian, X and Peng, Y and Liu, S and Shafiei, G and Wang, M and Sun, Y and Lou, J and Xian, J and Hu, K and He, Y and Wang, Q and Ding, C and Gao, T and Huang, S and Li, K and Wang, Q and Zuo, XN and Zhang, Z and Li, A and Liu, B},
title = {Spontaneous brain regional dynamics contribute to generalizable brain-behaviour associations.},
journal = {Nature human behaviour},
volume = {},
number = {},
pages = {},
pmid = {41168426},
issn = {2397-3374},
support = {82171543//National Natural Science Foundation of China (National Science Foundation of China)/ ; 82425024 & 82372049//National Natural Science Foundation of China (National Science Foundation of China)/ ; 20250484761//Beijing Nova Program/ ; 20230484425//Beijing Nova Program/ ; },
abstract = {Spontaneous brain activity is fundamental to understanding the neural basis of inter-individual differences, making its characterization central to brain-wide association studies. While inter-regional coupling patterns have been extensively studied, intra-regional dynamics remain largely unexplored. Here, analysing data from four neuroimaging cohorts (ages 8-82 years; N = 30,148), we extracted ~5,000 time-series features from resting-state haemodynamic signals across 271 brain regions, offering a comprehensive characterization of intra-regional dynamics. We identified a reliable subset that serves as an individual-specific 'barcode', capturing multifaceted dynamic dimensions that stably reflect inter-individual variation across datasets. These barcodes linked nonlinear autocorrelations in unimodal regions to substance use traits and random walk dynamics in higher-order networks to general cognitive abilities. Importantly, these brain-behaviour associations generalized across life stages and populations, with substance use showing age-specific variation and cognition exhibiting consistent patterns across age groups. This work advances large-scale, generalizable brain-wide association studies by highlighting the potential of intra-regional dynamics.},
}

@article {pmid41167558,
year = {2025},
author = {Zhang, Q and Li, M and Shen, L and Chen, L and Yuan, Z and Zhang, X and Wang, H and Yu, Y and Luo, F and Peng, G and Hu, J and Bao, Z and Song, N and Chen, K},
title = {A universal cost-efficient sample labeling approach for multiplexed single cell RNA-seq based on recombinant HUH-endonuclease-agglutinin tagging.},
journal = {Journal of genetics and genomics = Yi chuan xue bao},
volume = {},
number = {},
pages = {},
doi = {10.1016/j.jgg.2025.10.004},
pmid = {41167558},
issn = {1673-8527},
abstract = {Recent advances in single-cell transcriptomics have revolutionized our understanding of cellular diversity and tissue heterogeneity, providing unprecedented insights into biological and medical research. However, the high per-assay cost limits broader applications of this technology. Although sample-labeling strategies enabling multiplexing have emerged, current methods suffer from either impractical complexity for barcoding or high cost for preparing the index labeling reagents. To address these challenges, here we present HEATag, a universal cell membrane labeling approach that combines Duck circovirus HUH endonuclease (DCV) with wheat germ agglutinin (WGA) to efficiently tag cell membranes with indexed single-stranded DNA (indexed ssDNA). The DCV domain enables rapid, sequence-specific conjugation of indexed ssDNA, while the WGA domain ensures robust labeling of fresh or fixed cells across diverse species. This method is compatible with both commercial platforms and custom systems, readily adaptable to various single cell omics workflows. Therefore, HUH-endonuclease-agglutinin tagging (HEATag) provides a universal, cost-effective and scalable solution for high-throughput single-cell studies, enhancing library preparation efficiency and minimizing batch effects for single-cell researchers.},
}

@article {pmid41164792,
year = {2025},
author = {Suciu, SRA and Jung, JL and Azevedo, JMN},
title = {MONICEPH project: Monitoring cephalopods during whale-watching activity in the Azores (2020-2024).},
journal = {Biodiversity data journal},
volume = {13},
number = {},
pages = {e158822},
pmid = {41164792},
issn = {1314-2828},
abstract = {BACKGROUND: The study of oceanic cephalopods off the Azores Archipelago began decades ago with the analysis of stomach contents from sperm whales that were hunted for the whaling industry. The identification of numerous cephalopod species contributed significantly to cephalopod taxonomy, as well as enhancing understanding of the sperm whale diet. In the 1990s, the shift from whaling to whale-watching created new opportunities to continue studying deep-ocean ecology: participatory research involving the actors of the new industry.

NEW INFORMATION: MONICEPH (MONItoring CEPHalopods during whale-watching activity in the Azores) is a collaborative platform designed to collect, organise and disseminate cephalopod occurrence data gathered by whale-watching companies in the Azores. From 2020 to 2024, cephalopod remains found at the water's surface during sightings of cetaceans were collected in partnership with companies from four islands: São Miguel, Terceira, Pico and Faial. The deep-ocean cephalopod remains at the water's surface were likely brought up by their predators during feeding activity. We assume that sperm whales, in particular, occasionally release cephalopods at the surface due to incomplete consumption during a hunt or for feeding of their calves. Trained staff collected the samples, which were subsequently identified using DNA barcoding and/or morphological characteristics. The dataset includes 182 cephalopod records across 16 species. One species, Onykia carriboea Lesueur, 182, has been newly identified in the region, expanding the list of species previously documented in the published data for the Northeast Atlantic.},
}

@article {pmid41164282,
year = {2025},
author = {Almerekova, SS and Yermagambetova, MM and Yerbolatov, DY and Ishmuratova, MY and Turuspekov, YK},
title = {Complete plastome sequences of Lonicera L. species: implications for phylogeny and comparative analysis.},
journal = {Vavilovskii zhurnal genetiki i selektsii},
volume = {29},
number = {6},
pages = {883-895},
doi = {10.18699/vjgb-25-95},
pmid = {41164282},
issn = {2500-0462},
abstract = {Lonicera L. is one of the largest and economically significant genera in the family Caprifoliaceae Juss., with a controversial taxonomy. To contribute to its molecular taxonomy, we sequenced the plastomes of Lonicera species: Lonicera caerulea (two subspecies), L. tatarica, and L. micrantha - using next-generation sequencing technology and conducted a comparative analysis. Plastome sizes ranged from 153,985 bp in L. micrantha to 164,000 bp in L. caerulea subsp. pallasii, each containing 130 genes, including 85 protein-coding, 37 tRNA, and 8 rRNA genes. Five protein-coding (rps7, rps12, ndhB, ycf2, and ycf15), 7 tRNA (trnA-UGC, trnI-CAU, trnI-GAU, trnL-CAA, trnN-GUU, trnR-ACG, and trnV-GAC), and 4 rRNA (rrn4.5, rrn5, rrn16, and rrn23) genes were duplicated. Comparative analysis of Lonicera plastome boundaries revealed structural variations in L. caerulea subsp. altaica and L. caerulea subsp. pallasii, particularly in ndhA gene distribution. Three highly variable, two intergenic (ycf1-trnN-GUU and trnN-GUU-ndhF) and one genic (accD) region were identified. A total of 641 simple sequence repeats were detected in four plastomes. Phylogenetic analyses grouped Lonicera samples into two clades corresponding to subgenera Periclymenum and Chamaecerasus. In this study, the plastid genomes of two subspecies of L. caerulea and species L. micrantha were sequenced for the first time. The maximum likelihood tree derived from complete plastid genome sequences proved to be the most informative, showing a topology consistent with previous studies. The nucleotide sequences of variable regions (accD-ycf1-ndhF-trnN-GUU) demonstrate high potential for use in DNA barcoding and may serve as valuable molecular markers for species phylogenetic studies within the genus Lonicera.},
}

@article {pmid41162144,
year = {2025},
author = {Hutchings, P and McLaren, E and Kupriyanova, E and Gunton, LM and Lavesque, N and Przeslawski, R},
title = {The taxonomy of Australian polychaetes: current understanding, knowledge gaps and steps forward for management.},
journal = {Advances in marine biology},
volume = {101},
number = {},
pages = {27-56},
doi = {10.1016/bs.amb.2025.07.001},
pmid = {41162144},
issn = {2162-5875},
mesh = {Animals ; Australia ; *Polychaeta/classification ; *Conservation of Natural Resources ; *Biodiversity ; },
abstract = {Amid the current global biodiversity crisis, understanding and conserving marine invertebrates is more urgent than ever. Marine invertebrates are key components of biodiversity in benthic ecosystems, however, in Australia they remain underrepresented in biodiversity data and conservation planning. Australia's coastline extends from the tropics to subpolar environments and the surrounding waters comprise the Australian Territorial Seas and the Exclusive Economic Zone (EEZ). Currently there are 60 Australian Federal and 98 State Marine Parks which by law must ensure the sustainable management of their biodiversity. Polychaetes are dominant in benthic communities both in terms of species richness and diversity and play a critical role in the functioning of marine ecosystems. Polychaetes are also sensitive to environmental disturbance and change and must be considered in developing marine park zoning and monitoring plans. Here we review the history of species discovery (1791-2025) in shallow (0-300 m) and deep waters (300-5,000 m) within Australia. We highlight that many species remain undescribed, especially in the deep sea, and vast areas in Australian waters have little or no data on the polychaetes that occur there. Finally, we propose what can be done to ensure that polychaetes are included in management plans, including (1) increased availability of information for benthic ecologists from museums, (2) the development of reference DNA barcode libraries by museums, (3) increased tertiary education opportunities and (4) increased philanthropic funding sources.},
}

Animals
Australia
*Polychaeta/classification
*Conservation of Natural Resources
*Biodiversity

@article {pmid41162141,
year = {2025},
author = {Knorrn, AH and Sonnewald, M and Moctar, SMM and El-Hacen, EHM and Dia, M and Cherif, A and Sidi, MTO and Freiwald, A},
title = {The need for taxonomic expertise in protecting Mauritania's marine ecosystems and biological resources.},
journal = {Advances in marine biology},
volume = {101},
number = {},
pages = {105-151},
doi = {10.1016/bs.amb.2025.08.001},
pmid = {41162141},
issn = {2162-5875},
mesh = {Mauritania ; *Conservation of Natural Resources/methods ; Animals ; *Ecosystem ; *Biodiversity ; *Aquatic Organisms/classification ; },
abstract = {Seasonal upwelling events have a significant impact on the Mauritanian waters, which are a component of the Canary Current Large Marine Ecosystem (CCLME). These events frequently provide nutrient-rich waters to Mauritania's coastal waters. This influx of nutrients supports some of the most productive fish stocks in the Atlantic and sustains the development of a rich and diverse marine biodiversity. Despite its ecological and economic importance, a significant part of Mauritania's marine fauna remains insufficiently investigated, with significant taxonomic gaps spanning over a variety of taxa. A comprehensive understanding of regional biodiversity is essential for the implementation of sustainable fisheries management and the effective protection of marine ecosystems. Such understanding depends on accurate taxonomic knowledge, which forms the basis for assessing species distributions, ecological interactions and trophic networks. This review provides a synthesis of past research initiatives and campaigns conducted along the Mauritanian coast and identifies key coastal ecosystems of particular ecological relevance. It further highlights current gaps in taxonomic knowledge and points out the importance of an integrative approach to biodiversity research that combines classical morphological taxonomy with modern genetic species identification techniques. Additionally, the review advocates for the establishment and maintenance of a scientific reference collection of the Mauritanian marine fauna as a foundational resource for ongoing and future biodiversity assessments. Ultimately, this article proposes an integrative and interdisciplinary biodiversity research strategy for Mauritania's unique marine environment, thereby contributing to long-term conservation efforts and the sustainable use of marine resources at times of climate change and overexploitation of biological resources.},
}

Mauritania
*Conservation of Natural Resources/methods
Animals
*Ecosystem
*Biodiversity
*Aquatic Organisms/classification

@article {pmid41159105,
year = {2025},
author = {Hoxha, I and Dervović, J and Unterköfler, MS and Schlamadinger, L and Situmorang, T and Fuehrer, HP and Obwaller, AG and Sekulin, K and Camp, JV and Harl, J and Walochnik, J and Alić, A and Kniha, E},
title = {A cross section through mosquitoes of Bosnia and Herzegovina: Barcodes, blood meals and pathogens.},
journal = {One health (Amsterdam, Netherlands)},
volume = {21},
number = {},
pages = {101246},
pmid = {41159105},
issn = {2352-7714},
abstract = {Mosquitoes are important vectors of human and animal pathogens, yet data on their diversity and vector potential remain scarce for the central Balkan country Bosnia and Herzegovina. This study aimed to assess mosquito species composition, associated pathogens, and the potential public health risks in BIH. Adult mosquitoes were collected with light traps, identified morphologically and by barcoding, and screened molecularly for various pathogens, including West Nile virus (WNV), Dirofilaria spp., Trypanosoma spp., and Plasmodium spp. A total of 2691 mosquitoes of 17 species were identified, with Culex pipiens/torrentium being most abundant and new records of Aedes albopictus and Ae. japonicus japonicus. The first detection of WNV (lineage 2) RNA in mosquitoes in BIH highlights the potential risk of circulation in the region, aligning with findings from neighboring countries. In addition, DNA of filarioid nematodes (Dirofilaria immitis, D. repens, and Setaria tundra) were detected, underscoring their role as potential vectors of zoonotic dirofilariasis. Also, Trypanosoma and Plasmodium DNA was detected, warranting further investigation into the possible involvement of mosquitoes in their transmission. The detection of invasive Aedes species and mosquito-borne pathogens emphasize the need for strengthened vector surveillance in southeastern Europe, particularly in BIH. This study provides the first barcode inventory of 17 mosquito species and novel molecular evidence of mosquito-borne pathogens in BIH, offering valuable baseline data for future epidemiological assessments and sustained entomological surveillance.},
}

@article {pmid41152343,
year = {2025},
author = {Khode, PP and Sylvester, MN and Naumov, NG and Pethe, AM and Dhoble, SJ},
title = {Tuning photoluminescence in Gd2O3 via lattice engineering for advanced barcode and LEDs applications.},
journal = {Scientific reports},
volume = {15},
number = {1},
pages = {37706},
pmid = {41152343},
issn = {2045-2322},
abstract = {UNLABELLED: The utilization of luminescent phosphors in barcode technology can enhance the security feature. Using NaOH as a reducing agent, we have prepared Gd2O3:Eu[3+]/Er[3+] for luminescent phosphor. The cubic structure of Gd2O3 phosphor is confirmed by the structural analysis conducted using XRD. Dopant Eu and Er induce lattice contraction and expansion in the host lattice, respectively. Based on SEM analysis, porous and irregular particles with an average size of 401 nm were observed. In Tem analysis of prepared phosphor have some particle are in spherical in shape. The formation of Gd2O3 was confirmed by Gd-O band stretching as shown in the FTIR analysis. The emission in the red and green regions is emitted by Gd2O3:Eu[3+] and Gd2O3:Er[3+], respectively. The emission peaks of Gd2O3:Eu[3+]/Er[3+] are observed at 524 nm, 539 nm, 549 nm, 563 nm, 593 nm, 612 nm, and 629 nm when it is triggered by 363 nm, with a maximum energy transfer efficiency of 95.93%. Enhancing the security of barcodes can be achieved through the use of color-tunable Gd2O3:Eu[3+]/Er[3+] phosphor. Gd2O3:Er[3+] (1.5 mol%) is a prominent candidate for LED bulbs due to its CCT (5765 K).

SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1038/s41598-025-21434-3.},
}

@article {pmid41156631,
year = {2025},
author = {Munir, M and Naeem, M and Wu, X and Zeng, W and Sun, Z and Li, Y and Yong, T and Yang, F and Chang, X},
title = {Morpho-Molecular Identification and Pathogenic Characterization of Fusarium and Colletotrichum Species Associated with Intercropped Soybean Pod Decay.},
journal = {Pathogens (Basel, Switzerland)},
volume = {14},
number = {10},
pages = {},
doi = {10.3390/pathogens14101020},
pmid = {41156631},
issn = {2076-0817},
support = {2023YFD1401000//National Key R&D Program/ ; 23ZDYF3037//Key Research and Development Plan of Sichuan Province/ ; AB23026107//Guangxi Key Research and Development Program/ ; },
mesh = {*Glycine max/microbiology ; *Fusarium/genetics/classification/isolation & purification/pathogenicity ; *Colletotrichum/genetics/classification/pathogenicity/isolation & purification ; *Plant Diseases/microbiology ; Phylogeny ; China ; Seeds/microbiology ; DNA, Fungal/genetics ; },
abstract = {The fruiting stage of soybean (Glycine max L.) is critical for determining both its yield and quality, thereby influencing global production. While some studies have provided partial explanations for the occurrence of Fusarium species on soybean seeds and pods, the fungal diversity affecting soybean pods in Sichuan Province, a major soybean cultivation region in Southwestern China, remains inadequately understood. In this study, 182 infected pods were collected from a maize-soybean relay strip intercropping system. A total of 10 distinct pod-infecting fungal genera (132 isolates) were identified, and their pathogenic potential on soybean seeds and pods was evaluated. Using morphological characteristics and DNA barcode markers, we identified 43 Fusarium isolates belonging to 8 species, including F. verticillioides, F. incarnatum, F. equiseti, F. proliferatum, F. fujikuroi, F. oxysporum, F. chlamydosporum, and F. acutatum through the analysis of the translation elongation factor gene (EF1-α) and RNA polymerases II second largest subunit (RPB2) gene. Multi-locus phylogenetic analysis, incorporating the Internal Transcribed Spacer (rDNA ITS), β-tubulin (β-tubulin), Glyceraldehyde 3-phosphate dehydrogenase (GADPH), Chitin Synthase 1 (CHS-1), Actin (ACT), Beta-tubulin II (TUB2), and Calmodulin (CAL) genes distinguished 37 isolates as 6 Colletotrichum species, including C. truncatum, C. karstii, C. cliviicola, C. plurivorum, C. boninense, and C. fructicola. Among these, F. proliferatum and C. fructicola were the most dominant species, representing 20.93% and 21.62% of the isolation frequency, respectively. Pathogenicity assays revealed significant damage from both Fusarium and Colletotrichum isolates on soybean pods and seeds, with varying isolation frequencies. Of these, F. proliferatum, F. acutatum, and F. verticillioides caused the most severe symptoms. Similarly, within Colletotrichum genus, C. fructicola was the most pathogenic, followed by C. truncatum, C. karstii, C. cliviicola, C. plurivorum, and C. boninense. Notably, F. acutatum, C. cliviicola, C. boninense, and C. fructicola were identified for the first time as pathogens of soybean pods under the maize-soybean strip intercropping system in Southwestern China. These findings highlight emerging virulent pathogens responsible for soybean pod decay and provide a valuable foundation for understanding the pathogen population during the later growth stages of soybean.},
}

*Glycine max/microbiology
*Fusarium/genetics/classification/isolation & purification/pathogenicity
*Colletotrichum/genetics/classification/pathogenicity/isolation & purification
*Plant Diseases/microbiology
Phylogeny
China
Seeds/microbiology
DNA, Fungal/genetics

@article {pmid41154061,
year = {2025},
author = {Hu, J and Wei, H and Jiang, Y and Xue, Q and Wang, F},
title = {DNA Barcoding in Meat Authentication: Principles, Applications, and Future Perspectives.},
journal = {Foods (Basel, Switzerland)},
volume = {14},
number = {20},
pages = {},
doi = {10.3390/foods14203522},
pmid = {41154061},
issn = {2304-8158},
support = {ZCLZ24C1301//Basic Public Welfare Research Plan of Zhejiang Province, China, grant number/ ; 2023YW15//Fundamental Research Funds of China Jiliang University, grant number/ ; CY2023327//Zhejiang Provincial Administration for Market Regulation Eagle Plan Cultivation, grant number/ ; },
abstract = {DNA barcoding technology, as a species identification method based on specific DNA sequence variations, has been widely applied in meat product authentication in recent years. This paper reviews the technical principles, current applications, and comparative advantages of DNA barcoding in meat identification, particularly in contrast to traditional authentication methods. It further highlights the critical role of DNA barcoding in ensuring meat authenticity, enhancing food safety, and contributing to biodiversity conservation efforts. Furthermore, the paper explores the strategic implications and future trends of DNA barcoding in food regulation and ecological protection, demonstrating its practical feasibility and broad prospects in meat products. By highlighting its applications in detecting food adulteration and verifying species origin, this review aims to promote the safety and sustainable development of the meat industry while providing valuable insights for related fields. Ultimately, the implementation of DNA barcoding technology serves as a crucial safeguard for public food safety and health, aligning with the growing demand for improved food control systems.},
}

@article {pmid41153436,
year = {2025},
author = {de Virgilio, M and De Paola, D and Selvaggi, M and Carbonara, C and Ragni, M and Caputi Jambrenghi, A and Giannico, F and Colonna, MA and Tarricone, S},
title = {DNA Barcoding and Analysis of Nutritional Properties as a Tool for Enhancing Traceability of Anchovies (Engraulis encrasicolus L.) Fished in the Italian Southern Adriatic Sea.},
journal = {Genes},
volume = {16},
number = {10},
pages = {},
doi = {10.3390/genes16101219},
pmid = {41153436},
issn = {2073-4425},
support = {B91B17001140009//Regione Puglia/ ; },
mesh = {Animals ; *DNA Barcoding, Taxonomic/methods ; *Fishes/genetics/classification ; *Seafood/analysis ; Italy ; *Nutritive Value ; Mediterranean Sea ; Fatty Acids/analysis ; Cytochromes b/genetics ; DNA, Mitochondrial/genetics ; Seasons ; Pilot Projects ; },
abstract = {Background: Anchovies (Engraulis encrasicolus L.) are a component of the Mediterranean diet and among the most fished species. Despite Italian consumers showing a strong preference and willingness to pay more for locally caught anchovies, cases of mislabeling with non-local or different species have been documented. Molecular techniques like DNA barcoding offer reliable species identification, even in processed products, where morphological traits are no longer detectable. This pilot study applied a DNA barcoding technique targeting the mitochondrial cytochrome b gene to authenticate anchovies caught in the Italian Southern Adriatic Sea. Objectives: The study evaluated seasonal variations in the chemical and nutritional composition of anchovies, particularly the fatty acid profiles, highlighting their health benefits. Methods: During 2021, two fish samplings of anchovies were conducted per season from two fishing areas in Southern Adriatic Sea, one sample was used for mitochondrial DNA analyses, the other was used for morphometric measurements, physical, bromatological and chemical analyses. Results: Fish collected in summer showed higher total weight and edible yield relative to those fished in winter (p < 0.05). Anchovies fished in summer contained the highest concentration of proteins (p < 0.05) as compared to those caught during winter and autumn, while, in turn, they showed the highest amount of fat (p < 0.01). Fillets from anchovies fished during spring and summer contained a greater (p < 0.05) concentration of polyunsaturated fatty acids, and n-3 fatty acids than samples collected in autumn and winter. Conclusions: This study paves the way for further investigation to refine and validate the genetic identification and nutritional features of anchovies caught in the Italian Southern Adriatic Sea and marketed to consumers.},
}

Animals
*DNA Barcoding, Taxonomic/methods
*Fishes/genetics/classification
*Seafood/analysis
Italy
*Nutritive Value
Mediterranean Sea
Fatty Acids/analysis
Cytochromes b/genetics
DNA, Mitochondrial/genetics
Seasons
Pilot Projects

@article {pmid41153363,
year = {2025},
author = {Guo, X and Bai, Y and Ruan, J and Jin, X and Wang, S and Xue, D and Wu, Y},
title = {Comparative Analysis of Artemisia Plastomes, with Implications for Revealing Phylogenetic Incongruence and Evidence of Hybridization.},
journal = {Genes},
volume = {16},
number = {10},
pages = {},
doi = {10.3390/genes16101145},
pmid = {41153363},
issn = {2073-4425},
support = {32270215//National Natural Science Foundation of China/ ; LQ24C020002//Zhejiang Provincial Natural Science Foundation of China/ ; YB202406//University Laboratory Research Project of Zhejiang Province/ ; },
mesh = {*Artemisia/genetics/classification ; *Phylogeny ; *Hybridization, Genetic ; *Plastids/genetics ; Evolution, Molecular ; DNA Barcoding, Taxonomic ; Genome, Plant ; High-Throughput Nucleotide Sequencing ; },
abstract = {Background: With the advancement of the next-generation sequencing technology, it is becoming more cost-effective to obtain plastomes from genome skimming data at shallow sequencing depth. Artemisia is a species-rich genus, comprising species of great medicinal or economic value. However, plastomes of Artemisia have not been thoroughly and comparatively analyzed, and the phylogenetic relationships within the genus are still not well resolved. Methods: In this study, 19 Artemisia plastomes were obtained from genome skimming data. Together with the plastomes retrieved from the public database, comparative analyses of their structure were also conducted. We further used sequences of plastomes and nuclear internal transcribed spacer sequences to conduct phylogenetic reconstruction. Results: The Artemisia plastomes are conserved in terms of structure, GC content, gene number, and order. Some regions, i.e., accD, ccsA, ndhE, ycf1, ccsA-ndhD, trnG[GCC]-trnfM[CAU], were found to be variable and could be chosen as candidates for the DNA barcode. Phylogenetic analyses also confirmed that the four subgenera of Artemisia are not monophyletic. The incongruence between plastid and nuclear phylogenies indicated that hybridization events have occurred during the evolution of the genus. Conclusions: Reconstructed phylogenies using plastome sequences and nuclear internal transcribed spacers improved our understanding of the phylogenetic backbone of Artemisia. In the future, more taxa of Artemisia should be sequenced and analyzed to clarify the evolutionary history.},
}

*Artemisia/genetics/classification
*Phylogeny
*Hybridization, Genetic
*Plastids/genetics
Evolution, Molecular
DNA Barcoding, Taxonomic
Genome, Plant
High-Throughput Nucleotide Sequencing

@article {pmid41151664,
year = {2025},
author = {Laojun, S and Changbunjong, T and Kamoltham, T and Chaiphongpachara, T},
title = {An integrative approach to DNA barcoding, geometric morphometrics, and machine learning for field identification of Culex mosquitoes (Diptera: Culicidae), with implications for vector-borne disease surveillance.},
journal = {Acta tropica},
volume = {},
number = {},
pages = {107885},
doi = {10.1016/j.actatropica.2025.107885},
pmid = {41151664},
issn = {1873-6254},
abstract = {Culex mosquitoes are of considerable medical and veterinary importance, acting as vectors of arboviruses such as Japanese encephalitis, Rift Valley fever, and West Nile virus, as well as the filarial parasite Wuchereria bancrofti. Accurate identification of Culex species, however, remains challenging due to their close morphological similarity, frequent damage to field-collected specimens, and the limited availability of trained taxonomists. To address these challenges, this study employed an integrative framework combining DNA barcoding, wing geometric morphometrics (GM), and Random Forest (RF) to improve the identification of 12 Culex species (Cx. bicornutus, Cx. bitaeniorhynchus, Cx. brevipalpis, Cx. fuscocephala, Cx. gelidus, Cx. hutchinsoni, Cx. nigropunctatus, Cx. pseudovishnui, Cx. quinquefasciatus, Cx. sinensis, Cx. sitiens, and Cx. tritaeniorhynchus) in Thailand. DNA barcoding successfully validated the morphological identifications, with nucleotide sequences from representative specimens showing strong concordance with the GenBank and Barcode of Life Data Systems (BOLD) databases (≥97-100%), confirming the reliability of morphological diagnoses. Complementarily, wing GM demonstrated stronger discriminatory power: Mahalanobis distance analysis revealed all species to be significantly different (p < 0.05), and a cross-validated reclassification test achieved 82.18% performance with an adjusted total accuracy of 80%. For field identification of unknown specimens, both Mahalanobis distance and RF produced comparable results, yielding very high accuracy (80%-100%) for eight species. Overall, the integration of DNA barcoding, wing GM, and machine learning offers a robust and practical framework for strengthening mosquito-borne disease surveillance. Nonetheless, as each method has distinct strengths and limitations, their application should be carefully adapted to specific epidemiological and operational contexts.},
}

@article {pmid41151578,
year = {2025},
author = {Zhang, X and Wu, Z and He, H and Guan, Q and Ouyang, Q and Wang, R and Xie, L and Zhou, Y and Feng, B and Luo, Z and Xu, P and Yan, W and Hu, G and Li, J and Zhang, M and Zou, Y and Xu, X and Zhou, C and Cheng, Q and Liu, J and Gao, Q and Yang, S and Xiong, M and Chen, Y},
title = {3D-generation of high-purity midbrain dopaminergic progenitors and lineage-guided refinement of grafts supports Parkinson's disease cell therapy.},
journal = {Cell stem cell},
volume = {},
number = {},
pages = {},
doi = {10.1016/j.stem.2025.10.001},
pmid = {41151578},
issn = {1875-9777},
abstract = {The low in vivo yield of midbrain dopaminergic (mDA) neurons and uncertain lineage fates of donor cells following transplantation impede clinical application of human pluripotent stem cell (hPSC)-based cell therapy for Parkinson's disease (PD). We developed a three-dimensional (3D) differentiation method, SphereDiff, to generate high-purity mDA progenitors (mDAPs), leading to a significant enrichment of mDA neurons post transplantation. Grafted mDA neurons fully restored dopamine levels and corrected motor deficits in PD model mice. Single-cell spatial transcriptomics revealed a patterned distribution of mDA neuron subtypes and glial cells. Using cross-transplantation single-cell split barcoding (TX-SISBAR), we elucidated the clonal lineage fates of donor cells post transplantation, revealing the mDA neuron and astrocyte fates of mDAPs and glutamatergic neuron fates of diencephalic progenitors. Leveraging these lineage insights, we further refined SphereDiff and eliminated off-target lineage cells. Producing high in vivo efficacy, lineage-defined donor cells supports safer and more effective PD cell therapy in regenerative medicine.},
}

@article {pmid41148924,
year = {2025},
author = {Zangl, L and Fischer, I and Sittenthaler, M and Chovanec, A and Gros, P and Holzinger, W and Kunz, G and Lienhard, A and Macek, O and Mayerhofer, C and Mladinić, M and Topić, M and Schäffer, S and Sefc, KM and Sturmbauer, C and Haring, E and Koblmüller, S},
title = {A DNA Barcode Inventory of Austrian Dragonfly and Damselfly (Insecta: Odonata) Species.},
journal = {Insects},
volume = {16},
number = {10},
pages = {},
pmid = {41148924},
issn = {2075-4450},
support = {na//Austrian Federal Ministry of Science, Research and Economy/ ; W-UNS-01/17//Municipal Department 22 - Environmental Protection (MA22)/ ; W-UNS-01/17//European Agricultural Fund for Rural Development 2014-2020/ ; },
abstract = {Dragonflies and damselflies are important indicator species for quality and health of (semi-)aquatic habitats. Hitherto, 78 species of Odonata have been reported for Austria. Ecological data, Red List assessments, and a dragonfly association index exist, but population- and species-level genetic data are largely lacking. In this study, we establish a comprehensive reference DNA barcode library for Austrian dragonflies and damselflies based on the standard barcoding marker COI. Because of the increasing significance of environmental DNA (eDNA) analyses, we also sequenced a segment of the mitochondrial 16S rRNA gene, a marker often used in eDNA metabarcoding approaches. In total, we provide 786 new COI barcode sequences and 867 new 16S sequences for future applications. Sequencing success was >90 percent for both markers. Identification success was similar for both markers and exceeded 90 percent. Difficulties were only encountered in the genera Anax Leach, 1815, Chalcolestes Kennedy, 1920, Coenagrion Kirby, 1890 and Somatochlora Selys, 1871, with low interspecific genetic distances and, consequently, BIN (barcode index number) sharing. In Anax, however, individual sequences clustered together in species-specific groups in the COI tree. Irrespective of these challenges, the results suggest that both markers perform well within most odonate families in terms of sequencing success and species identification and can be used for reliably delimiting Austrian species, monitoring, and eDNA approaches.},
}

@article {pmid41148905,
year = {2025},
author = {Melone, G and Andretta, L and Guastaferro, VM and Romito, E and Formisano, G and Giorgini, M and Laudonia, S},
title = {The Parasitoid Complex of Aleurothrixus floccosus (Hemiptera: Aleyrodidae) in the Citrus Groves of Central-Southern Italy.},
journal = {Insects},
volume = {16},
number = {10},
pages = {},
doi = {10.3390/insects16101037},
pmid = {41148905},
issn = {2075-4450},
support = {CUP B29I22001290009//U.R.Co.Fi (Regional Phytosanitary Coordination Unit) funded by the government of the Campania Region of Italy/ ; },
abstract = {The woolly whitefly, Aleurothrixus floccosus, is likely a Neotropical origin species that has spread globally. Introduced to France in 1969, it became a pest in southern European citrus groves, first reported in Italy in 1974. Integrated management using biological control agents is crucial due to the low efficacy of chemical controls. Nymphs produce waxy filaments and honeydew, limiting insecticide contact. Natural enemies, especially from Neotropics, have been documented. The parasitoids Amitus spiniferus and Cales noacki were released in France in 1970 and later observed in Liguria, Italy. In the Campania region, C. noacki was first found on Aleurotuba jelineki in 1984 and this finding preceded the first report of A. floccosus in the same area. Subsequently, C. noacki was also introduced in other regions where it showed better adaptation throughout the Italian territory, reaching high parasitization levels on the woolly whitefly nymphs. After many years since the last field investigations, surveys in 2024-2025 in organic citrus groves in central and southern Italy identified additional parasitoids. Besides C. noacki and A. spiniferus, Eretmocerus paulistus and Signiphora xanthographa were found for the first time in Italy. Both species were originally described from the Neotropical ecozone. The aphelinid finding represents its first documented establishment in Italy, while the signiphorid one represents a new record for the European fauna. E. paulistus is a primary parasitoid, while S. xanthographa is a hyperparasitoid that can limit the effectiveness of other parasitoids. The interaction of these parasitoids resulted in high parasitism rates for A. floccosus nymphs. Preserving the current complexity of parasitoids in integrated pest management (IPM) programs could effectively control the woolly whitefly in central and southern Italy.},
}

@article {pmid41148902,
year = {2025},
author = {Thongsahuan, S and Aupalee, K and Yakoh, A and Kaewnoi, D and Maleewong, W and Srisuka, W and Wannasan, A and Saeung, A and Takaoka, H},
title = {Integrative Description and Redescription of Black Fly (Diptera: Simuliidae) Species in the Simulium (Gomphostilbia) ceylonicum Species-Group from Thailand.},
journal = {Insects},
volume = {16},
number = {10},
pages = {},
doi = {10.3390/insects16101034},
pmid = {41148902},
issn = {2075-4450},
support = {N42A670561//National Research Council of Thailand/ ; },
abstract = {Utilizing the COI barcoding approach, cryptic diversity has previously been detected within the morphologically recognized Simulium (Gomphostilbia) trangense Jitklang, Kuvangkadilok, Baimai, Takaoka & Adler, 2008 and S. (G.) sheilae Takaoka & Davies, 1995, of the S. (G.) ceylonicum species-group. Here, an unknown black fly species belonging to the S. ceylonicum species-group from southern Thailand was discovered and described as a new species, S. (G.) sipoense sp. nov. In addition, S. (G.) trangense is herein fully redescribed based on specimens collected from its type locality. Based on an integrative taxonomic approach combining morphological and molecular data, the validity of the newly described S. sipoense sp. nov. and the redescribed S. trangense is confirmed. Comparative morphological characteristics and phylogenetic analysis, inferred from COI sequences, suggest that the new species is conspecific with the species redescribed as S. trangense, using specimens collected from Malaysia, and is morphologically and phylogenetically closely related to S. sheilae, particularly to the specimens from Indonesia. The redescribed S. trangense is genetically highly similar or even identical to the species that was apparently misidentified as S. sheilae from southern and western Thailand, and is morphologically very similar to the new species, from which it is clearly distinguished by the relative length of the female claw tooth, shape of the male ventral plate, and color of the larval body. A detailed information on the morphological characteristics separates the new species, and the redescribed S. trangense from all other known species of the same species-group in Thailand and neighboring countries is provided. Further studies are warranted to clarify the taxonomic status of several cryptic species recognized within the morphologically defined S. trangense and S. sheilae.},
}

@article {pmid41148898,
year = {2025},
author = {Kasparek, M and Schmid-Egger, C and Roberts, H},
title = {Cryptic and Non-Cryptic Diversity in Cleptoparasitic Bees of the Genus Stelis Panzer, 1806, Subgenus Stelidomorpha Morawitz, 1875, with a Description of New Species from the Arabian Peninsula (Hymenoptera, Megachilidae).},
journal = {Insects},
volume = {16},
number = {10},
pages = {},
doi = {10.3390/insects16101030},
pmid = {41148898},
issn = {2075-4450},
abstract = {Cleptoparasitic bees of the subgenus Stelis (Stelidomorpha) occur mainly in the Mediterranean and Middle East. In this study, we elevate Stelis aegyptiaca ssp. canaria Warncke, 1992 to species rank (S. canaria Warncke, 1992) and describe two new species, Stelis alainensis Kasparek sp. nov. and Stelis surica Kasparek sp. nov., both discovered in Oman and the United Arab Emirates. Morphological differences between these species and their closest relatives (S. aegyptiaca Radoszkowski, 1876, S. pentelica Mavromoustakis, 1963, and S. nasuta (Latreille, 1809)) are corroborated by genetic divergence in the mitochondrial COI barcode region, with Kimura 2-parameter (K2P) distances of 7.6-15.2%. A notable case is Stelis nasuta, which shows deep genetic subdivision into three clusters: (1) Iberian Peninsula and North Africa, (2) southeastern France, Italy, and the Balkans, (3) eastern Balkans, Turkey, and the Levant. Moderate genetic K2P distances of 2.9-3.3% complicated species delimitation. Analyses with ABGD, ASAP, bPTP, and RESL algorithms consistently supported recognition of these lineages as putative species. As multivariate analyses of 11 morphometric traits revealed no consistent diagnostic differences, we treat these lineages as phylospecies rather than formal taxa. Our findings demonstrate that bee diversity in the Palaearctic remains underestimated, and that expanded sampling and integrative approaches continue to reveal hidden lineages.},
}

@article {pmid41148888,
year = {2025},
author = {Yetchom Fondjo, JA and Wandji, AC and Zahiri, R and Hawlitschek, O and Hemp, C},
title = {Integrative Taxonomy Revealed Cryptic Diversity in the West African Grasshopper Genus Serpusia Karsch, 1891 (Orthoptera: Catantopinae).},
journal = {Insects},
volume = {16},
number = {10},
pages = {},
doi = {10.3390/insects16101020},
pmid = {41148888},
issn = {2075-4450},
abstract = {BACKGROUND/OBJECTIVES: Despite their ecological significance, DNA barcoding data for African rainforest Orthoptera remain underrepresented globally, limiting progress in species discovery, biodiversity assessment, and conservation. This study aimed to generate molecular data for morphologically identified Serpusia Karsch, 1891 species to evaluate their taxonomic status.

METHODS: Specimens were collected from multiple sites in Cameroon and analyzed using DNA barcoding with COI-5P and 16S rDNA markers. Species delimitation was performed with Automatic Barcode Gap Discovery, and phylogenetic relationships were inferred using Maximum Likelihood and Bayesian Inference. Additionally, external morphology and the male phallic complex were examined.

RESULTS: Molecular analyses delineated 19 MOTUs, five corresponding to Serpusia opacula, seven to Serpusia succursor and the remainder to outgroups. Similarity-based assignments matched these MOTUs to 19 BINs. Phylogenetic reconstruction revealed S. opacula and S. succursor as two genetically distinct clades, with the S. opacula group more closely related to Aresceutica Karsch, 1896 than to the S. succursor group. Accordingly, we established a new genus, Paraserpusia gen. nov., to accommodate S. succursor. Within the S. opacula group, five species are recognized: one previously described (S. opacula) and four new species (S. kennei sp. nov., S. missoupi sp. nov., S. seinoi sp. nov., and S. verhaaghi sp. nov.). The former S. succursor, now Paraserpusia succursor, is divided into six well-supported lineages, five of which are formally described here (P. hoeferi sp. nov., P. husemanni sp. nov., P. kekeunoui sp. nov., P. tamessei sp. nov., and P. tindoi sp. nov.). A haplotype network based on COI-5P sequences corroborates three major clades corresponding to the S. opacula group, the S. succursor group, and Aresceutica. Diagnostic morphological differences between Serpusia and Paraserpusia are consistently supported across characters.

CONCLUSIONS: This integrative approach reveals substantial hidden diversity within Serpusia and highlights the importance of combining molecular and morphological data to uncover and formally describe previously overlooked taxa.},
}

@article {pmid41147142,
year = {2025},
author = {Yadav, V and Tiwari, AK and Siddhanta, S},
title = {Generative Adversarial Network-driven high-resolution Raman spectral generation for accurate molecular feature recognition.},
journal = {The Analyst},
volume = {},
number = {},
pages = {},
doi = {10.1039/d5an00354g},
pmid = {41147142},
issn = {1364-5528},
abstract = {Through the probing of light-matter interactions, Raman spectroscopy provides invaluable insights into the composition, structure, and dynamics of materials. Obtaining such data from portable and cheap instruments is of immense practical relevance in several domains. Here, we propose the integration of a Generative Adversarial Network (GAN) to generate high-resolution Raman spectra with a portable hand-held spectrometer to facilitate concurrent spectral analysis and compound classification. Portable spectrometers generally have a lower resolution, and the Raman signal is usually buried under the background noise. The GAN-based model could not only generate high-resolution data but also reduce the spectral noise significantly. The generated data was further tested on a trained Artificial Neural Network (ANN) model for the classification of organic and pharmaceutical drug molecules, which was further used for spectral barcoding for the identification of unknown pharmaceutical drugs. This integrated system holds the potential for achieving accurate and real-time monitoring of noisy inputs to obtain high throughput output, thereby opening new avenues for applications in different domains. This synergy between spectroscopy and machine learning (ML) facilitates improved data processing, noise reduction, and feature extraction and paves the way for predictive modeling and automated decision-making using cost-effective portable devices.},
}

@article {pmid41145626,
year = {2025},
author = {Wang, Y and Salama, YE and Amer, KE and Elsayed, WF and Orabi, SA and El-Sappah, AH and Mahdy, EMB and Zayed, EM and Elashtokhy, MMA},
title = {Molecular and agronomic assessment of faba bean genotypes identifies resistance to Orobanche crenata infestation.},
journal = {Scientific reports},
volume = {15},
number = {1},
pages = {37535},
pmid = {41145626},
issn = {2045-2322},
mesh = {*Vicia faba/genetics/parasitology/growth & development ; *Orobanche/physiology ; Genotype ; *Plant Diseases/genetics/parasitology ; *Disease Resistance/genetics ; },
abstract = {Faba bean (Vicia faba L.)(V. faba), an essential legume in Egypt, is severely impacted by broomrape (Orobanche crenata) (O. crenata), a parasitic weed that feeds on roots, making chemical control difficult without harming the crop. Three faba bean genotypes (Giza-843, Misr-3, and Sakha-3) along with nine pure lines were evaluated for resistance to broomrape in field trials (2021-2023) using gene barcoding (rbcL, rpoC1), ten Start Codon Targeted (SCoT) markers, and phonological and agronomic traits to identify resistance sources. Five faba bean genotypes-Giza 843, Lines 2, 7, 8, and 9-significantly reduced all broomrape growth and productivity traits. Line 5 had the lowest spike length, fresh and dry spike weight, number of spikes/m[2], and number of capsules/spike across both seasons. Genotypes Line 5, Line 6, and Misr 3 exhibited the highest seed yield per plot and hectare under normal and broomrape-infested conditions. Line 4 showed the lowest yield reductions, followed by Lines 9 and 8. The study generated 103 amplicons with a polymorphism rate of 51.46%, with SCoT-10 being the most informative marker, revealing 69% polymorphism and affecting 13 amplicons. Three faba bean genotypes showed significant genetic diversity, high seed output, and effectively reduced broomrape growth, highlighting their potential for genetic improvement and sustainable use in future research. Four faba bean genotypes, namely Sakha 3, Line 2, Line 4, and Line 9, are promising genotypes for combating broomrape infestation.},
}

*Vicia faba/genetics/parasitology/growth & development
*Orobanche/physiology
Genotype
*Plant Diseases/genetics/parasitology
*Disease Resistance/genetics

@article {pmid41145427,
year = {2025},
author = {van der Nol, E and Haupt, NA and Gao, QQ and Smit, BAM and Hoffmann, MA and Engler-Lukajewski, M and Ludwig, M and McKenna, S and Mata, JM and Béquignon, OJM and van Westen, G and Wendel, TJ and Noordermeer, SM and Böcker, S and Pomplun, S},
title = {Barcode-free hit discovery from massive libraries enabled by automated small molecule structure annotation.},
journal = {Nature communications},
volume = {16},
number = {1},
pages = {9479},
pmid = {41145427},
issn = {2041-1723},
support = {VFE 0003//EC | European Regional Development Fund (Europski Fond za Regionalni Razvoj)/ ; VFE 0029//EC | European Regional Development Fund (Europski Fond za Regionalni Razvoj)/ ; 101039354//EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020)/ ; },
mesh = {*Small Molecule Libraries/chemistry/pharmacology ; *Drug Discovery/methods ; Humans ; Carbonic Anhydrase IX/antagonists & inhibitors/metabolism ; Tandem Mass Spectrometry ; Software ; },
abstract = {Affinity-selection platforms are powerful tools in early drug discovery, but current technologies - most notably DNA-encoded libraries (DELs) - are limited by synthesis complexity and incompatibility with nucleic acid-binding targets. We present a barcode-free self-encoded library (SEL) platform that enables direct screening of over half a million small molecules in a single experiment. SELs combine tandem mass spectrometry with custom software for automated structure annotation, eliminating the need for external tags for the identification of screening hits. We develop efficient, high-diversity synthesis protocols for a broad range of chemical scaffolds and benchmark the platform in affinity selections against carbonic anhydrase IX, identifying multiple nanomolar binders. We further apply SELs to flap endonuclease 1 (FEN1) - a disease related DNA-processing enzyme inaccessible to DELs - and discover potent inhibitors. Taken together, screening barcode-free libraries of this scale all at once represents an important development, enables access to novel target classes, and promises substantial impact on both academic and industrial early drug discovery.},
}

*Small Molecule Libraries/chemistry/pharmacology
*Drug Discovery/methods
Humans
Carbonic Anhydrase IX/antagonists & inhibitors/metabolism
Tandem Mass Spectrometry
Software

@article {pmid41143289,
year = {2025},
author = {Sosa, SOSA and Andrade, LF and Boyko, CB and Brandt, A and Buge, B and Dávila Jiménez, Y and Henseler, M and Hernández Alcántara, P and Jóźwiak, P and Knauber, H and Marcondes Machado, F and Martínez-Muñoz, CA and Momtazi, F and Nakadera, Y and Qiu, JW and Riehl, T and Rouse, GW and Sigwart, JD and Sirenko, B and Souza-Filho, JF and Steger, J and Stępień, A and Tilic, E and Trautwein, B and Vončina, K and Williams, JD and Zhang, J},
title = {Ocean Species Discoveries 13-27 - Taxonomic contributions to the diversity of Polychaeta, Mollusca and Crustacea.},
journal = {Biodiversity data journal},
volume = {13},
number = {},
pages = {e160349},
pmid = {41143289},
issn = {1314-2828},
abstract = {BACKGROUND: Despite centuries of exploration, marine invertebrate biodiversity remains notably under-described. The majority of species in major marine groups are still unnamed, limiting our ability to understand and conserve ecosystems facing rapid environmental change. The rate of species discovery continues to outpace the formal process of species description. This gap creates an urgent need for streamlined, scalable approaches to taxonomy. The SENCKENBERG OCEAN SPECIES ALLIANCE was founded to help meet this challenge by facilitating global collaboration, offering technical support for species documentation and promoting efficient taxonomic publishing. Within this framework, Ocean Species Discoveries provides a forum for concise, but data-rich descriptions of marine invertebrate taxa. This second collection presents a diverse set of taxonomic contributions, based on recent and historical collections, including newly-described species and a re-description of a previously poorly-known taxon. The integrative documentation of the taxa treated herein was facilitated by the newly-established Discovery Laboratory at the Senckenberg Research Institute, the first service unit dedicated to supporting alpha taxonomists.

NEW INFORMATION: This article presents 14 new species and one re-description, two new genera, with taxa spanning three phyla. Newly-described taxa comprise two polychaete annelids: Nicon salinus Hernández-Alcántara & Dávila-Jiménez, sp. nov. and Spinther bohnorum Tilic & Rouse, sp. nov. Molluscs span four classes, with three polyplacophorans: Craspedochiton zefranki Vončina, sp. nov., Ferreiraella charazata Sigwart, sp. nov. and a new genus with type species Pycnodontochiton sinensis Sirenko, Zhang & Sigwart, gen. et sp. nov. and Pycnodontochiton tenuidontus (Saito and Okutani, 1990), comb. nov. The new monoplacophoran Veleropilina gretchenae Sigwart & Steger, sp. nov. is one of the first species of this class with a high-quality genome, published from the specimen that is now the holotype. The scaphopod Laevidentalium wiesei Sahlmann, 2012 represents a re-description and range extension and the bivalve Myonera aleutiana Machado & Sigwart, sp. nov. is the second bivalve including an anatomical description with non-invasive methods using micro-CT. Amongst crustaceans, there are two new amphipod species: Apotectonia senckenbergae Momtazi & Riehl, sp. nov. and Metharpinia hirsuta Souza-Filho & Andrade, sp. nov. Three isopod species were described, including the parasitic species Zeaione everta Boyko & Williams, sp. nov. that is the only species in the new genus Zeaione Boyko & Williams, gen. nov. and two free-living isopods: Haploniscus bulbosus Henseler, Knauber & Riehl, sp. nov. and Macrostylis peteri Riehl, sp. nov. Finally, there are two new tanaidaceans: Hoplopolemius olo Jóźwiak & Stępień, sp. nov. and Nesotanais thalassinus Stępień, sp. nov.The data used for the description of ten of the species and one of the new genera treated herein were wholly or partially obtained at the SOSA Discovery Laboratory using integrative methods including light and electron microscopy, confocal imaging, molecular barcoding and micro-CT scanning. Additional novel findings include the first record of the family Macrostylidae and the genus Macrostylis G. O. Sars, 1864 from Australian waters (Macrostylis peteri, sp. nov.) and novel host associations: Ferreiraella charazata, sp. nov. is documented with epibiotic tubeworms on its tail valves that are typical of this genus and the decapod Eucalliaxiopsis aequimana (Baker, 1907) is newly recorded as a host for bopyrid isopods, representing the first such record for the family Eucalliacidae.},
}

@article {pmid41143268,
year = {2025},
author = {Cu, HL and Nguyen, HD and Pham, LT and Chu, MH},
title = {Dataset on some chloroplast DNA regions of Strobilanthes bantonensis Lindau in Vietnam.},
journal = {Data in brief},
volume = {62},
number = {},
pages = {111932},
pmid = {41143268},
issn = {2352-3409},
abstract = {Strobilanthes bantonensis is used in traditional medicine to treat gastritis, lung inflammation, high fever, heat reduction, and tumors. S. bantonensis is a plant species of scientific interest for the discovery of novel physiologically active compounds. The Strobilanthes species exhibit considerable morphological similarity and significant homoplasy, which together with deformed or powdered specimens, complicate further classification and distinction. This dataset contains the nucleotide sequences of PCR-specific primer pairs, the sequences of the trnE-trnT, psbK-psbI, ycf1, and matK regions isolated from the S. bantonensis, and the phylogenetic trees constructed from the sequences of these chloroplast DNA regions. In the phylogenetic trees constructed based on the two intergenic regions, trnE-trnT and psbK-psbI, and the matK gene region, S. bantonensis (CB_VN) is in the same subgroup as S. bantonensis (GenBank: MT576695.1), with bootstrap coefficients of 100 %, 100 %, and 98 %, respectively. Notably, in all three phylogenetic trees, S. bantonensis (CB_VN) is distributed in the monophyletic group (Strobilanthes). The trnE-trnT, psbK-psbI, and matK markers are viable candidates for DNA barcoding to assist in the identification of Strobilanthes species. The primer pairs trnE-trnT_F/trnE-trnT_R, psbK-psbI_F/psbK-psbI_R, ycf1_F/ycf1_R, and matK_F/matK_R can be used to amplify the trnE-trnT, psbK-psbI, ycf1, and matK regions from species of the Strobilanthes genus, Acanthaceae family and other higher plants. This dataset is the first report on the trnE-trnT, psbK-psbI, ycf1, and matK markers and the phylogeny based on these sequences of S. bantonensis.},
}

@article {pmid41142504,
year = {2025},
author = {Chen, W and Song, S and Samanta, A and Sethi, S and Drees, C and Kappl, M and Butt, HJ and Walther, A},
title = {Growing functional artificial cytoskeletons in the viscoelastic confinement of DNA synthetic cells.},
journal = {Nature chemical engineering},
volume = {2},
number = {10},
pages = {627-639},
pmid = {41142504},
issn = {2948-1198},
abstract = {Intracellular structures, such as cytoskeletons, form within a crowded cytoplasm with viscoelastic properties. While self-assembly in crowding is well studied, the effects of coupled viscoelastic environments remain elusive. Here we engineer all-DNA synthetic cells (SCs) with tunable viscoelastic interiors to investigate this phenomenon. We introduce facile DNA barcode engineering to selectively enrich DNA tiles with adjustable concentrations into SCs to form artificial cytoskeletons coupled to their interior. Distinct mechanistic differences in assembly occur compared with solution or simple crowding. Furthermore, we develop light, molecular and metabolic switches to direct structure formation and create self-sorted SC populations with distinct artificial cytoskeletons. These cytoskeletons strengthen SCs and support stable contacts with mammalian cells. By bridging molecular-scale DNA nanotube assembly with mesoscale condensate structures, our SCs provide a versatile platform to investigate self-assembly under viscoelastic confinement and to harness subcellular architectures for emerging applications.},
}

@article {pmid41140631,
year = {2025},
author = {Wen, B and Cheng, R},
title = {A taxonomic study of Gandaritis flavomacularia and related species (Lepidoptera, Geometridae), with description of a new species from western China.},
journal = {ZooKeys},
volume = {1255},
number = {},
pages = {333-341},
pmid = {41140631},
issn = {1313-2989},
abstract = {The taxonomic status of Gandaritis flavomacularia has recently undergone revision. This study evaluates the validity of G. flavomacularia based on both morphological and molecular evidence. Furthermore, a new, closely related species, Gandaritis stueningi Wen & Cheng, sp. nov. from Sichuan, China, is described, supported by both molecular and morphological data. Key morphological characters, including the male and female genitalia, are illustrated and compared with those of three related species.},
}

@article {pmid41139833,
year = {2025},
author = {Neo, DM and Ben-Zion, I and Bagnall, J and Solomon, MY and Bond, AN and Gath, E and Zhang, S and Shoresh, N and Gomez, J and Hung, DT},
title = {A Multiplexed, Target-Based Phenotypic Screening Platform Using CRISPR Interference in Mycobacterium abscessus.},
journal = {ACS infectious diseases},
volume = {},
number = {},
pages = {},
doi = {10.1021/acsinfecdis.5c00623},
pmid = {41139833},
issn = {2373-8227},
abstract = {The rise of difficult-to-treat Mycobacterium abscessus infections presents a growing clinical challenge due to the immense arsenal of intrinsic, inducible and acquired antibiotic resistance mechanisms that render many existing antibiotics ineffective against this pathogen. Moreover, the limited success in discovery of novel compounds that inhibit novel pathways underscores the need for innovative drug discovery strategies. Here, we report a strategic advancement in PROSPECT (PRimary screening Of Strains to Prioritize Expanded Chemistry and Targets), which is an antimicrobial discovery strategy that measures chemical-genetic interactions between small molecules and a pool of bacterial mutants, each depleted of a different essential protein target, to identify whole-cell active compounds with high sensitivity. Applying this modified strategy to M. abscessus, in contrast to previously described versions of PROSPECT which utilized protein degradation or promoter replacement strategies for generating engineered hypomorphic strains, here we leveraged CRISPR interference (CRISPRi) to more efficiently generate mutants each depleted of a different essential gene involved in cell wall synthesis or located at the bacterial surface. We applied this platform to perform a pooled PROSPECT pilot screen of a library of 782 compounds using CRISPRi guides as mutant barcodes. We identified a range of active hits, including compounds targeting InhA, a well-known mycobacterial target but under-explored in the M. abscessus space. The unexpected susceptibility to isoniazid, traditionally considered to be ineffective in M. abscessus, suggested a complex interplay of several intrinsic resistance mechanisms. While further complementary efforts will be needed to change the landscape of therapeutic options for M. abscessus, we propose that PROSPECT with CRISPRi engineering provides an increasingly accessible, high-throughput target-based phenotypic screening platform and thus represents an important step toward accelerating early stage drug discovery.},
}

@article {pmid41138890,
year = {2025},
author = {Rodrigues Alves, MT and Aluotto Scalzo Júnior, SR and Nunes da Silva, W and de Almeida Costa, RA and Dias Moura Prazeres, PH and de Oliveira Costa, RB and Cordeiro Guimarães, L and de Oliveira Guarnieri, L and Marta Figueiredo, M and de Araujo Moreira, F and de Toledo Ribas, V and Ricardo Massensini, A and Goulart Guimarães, PP},
title = {High-Throughput in vivo and in vitro screening of lipid nanoparticles for nucleic acid delivery to the brain.},
journal = {International journal of pharmaceutics},
volume = {},
number = {},
pages = {126278},
doi = {10.1016/j.ijpharm.2025.126278},
pmid = {41138890},
issn = {1873-3476},
abstract = {Gene therapy is a promising approach for correcting acquired or inherited brain diseases, nevertheless, faces a challenge in effectively delivering nucleic acids to the brain. Ionizable lipid nanoparticles (LNPs) are commonly used as delivery systems, however they are often screened in in vitro settings which poorly replicates in vivo biological barriers. Here, we used a high-throughput in vivo and in vitro screening methods to assess a library of LNPs for nucleic acid delivery to neurons and brain tissue. LNPs were formulated via microfluidic mixing with different helper lipid and molar ratios, each containing a unique barcode DNA (b-DNA). LNPs were characterized and pooled for intravenous injection in C57BL/6 mice, and their biodistribution was assessed via next-generation sequencing. The top-performing LNPs, which exhibited higher b-DNA delivery to the brain, were further assessed for transfection efficiency in primary neurons using pDNA and mRNA. We established concentration-response curves, monitored protein expression overtime, and performed cell viability assays. Our results showed that DOPE-based LNPs outperformed other formulations in brain delivery and neuronal transfection. Additionally, altering the ionizable lipid in our top formulation to FDA-approved options did not improve neuronal transfection efficiency. Finally, our identified top performing LNP4 was able to induce luciferase expression in brain after intravenous administration. No signs of neurological damage, inflammation or behavioral impairments were observed. Our results demonstrate that our LNPs efficiently deliver nucleic acids to neurons and the brain, while our screening strategy accelerates the design of LNPs for brain gene therapy.},
}

@article {pmid41134309,
year = {2025},
author = {Cardeñosa, D and Grogan, PA and Chapman, DD},
title = {Bite, swab, identify: Validating molecular tools for detecting depredating shark species.},
journal = {Journal of fish biology},
volume = {},
number = {},
pages = {},
doi = {10.1111/jfb.70264},
pmid = {41134309},
issn = {1095-8649},
support = {//The Roe Foundation/ ; },
abstract = {Depredation, the partial or complete removal of hooked fish by predators, poses significant challenges for fisheries worldwide. Shark depredation in recreational fisheries has become a growing concern, influencing both fisheries management and public perceptions of shark conservation. Although DNA swabbing of depredated fish has been used to identify responsible shark species, the reliability of this technique remains uncertain due to potential environmental DNA (eDNA) contamination. In this study, we evaluated the accuracy of swab-based molecular identification by comparing genetic results to video-confirmed depredation events. Four depredation events were recorded in Jupiter, Florida, involving a bull shark (Carcharhinus leucas), lemon shark (Negaprion brevirostris), great hammerhead shark (Sphyrna mokarran) and great barracuda (Sphyraena barracuda). DNA analysis of swab samples from depredated fish correctly identified the responsible shark species in all three shark cases. Barracuda depredation yielded no amplification. Our findings validate the use of swab-based molecular techniques for accurately identifying depredating shark species and confirm the absence of misleading eDNA signals. This approach provides a valuable, non-invasive tool for studying predator-prey interactions and informing fisheries management strategies.},
}

@article {pmid41133790,
year = {2025},
author = {Bonneau, P and Renkema, JM and Gariepy, T and Firlej, A and Fournier, V},
title = {Effects of multiple biological control agents on Drosophila suzukii (Diptera: Drosophilidae) in the laboratory, greenhouse, and field.},
journal = {Journal of economic entomology},
volume = {},
number = {},
pages = {},
doi = {10.1093/jee/toaf245},
pmid = {41133790},
issn = {1938-291X},
support = {IA116618//Programme Innov'Action Agroalimentaire/ ; //Centre SÈVE/ ; },
abstract = {Drosophila suzukii Matsumura is a nearly worldwide invasive pest that causes damage to ripening fruit, particularly raspberries. Biological control in an integrated pest management strategy may be an alternative to repeated insecticide applications. We evaluated the effects of using multiple commercially available agents against D. suzukii in raspberries. In the laboratory, the combination of the pupal parasitoid Muscidifurax raptorellus Kogan and Legner (Hymenoptera: Pteromalidae) and predators Orius insidiosus Say (Heteroptera: Anthocoridae) and Chrysoperla carnea Stephens (Neuroptera: Chrysopidae) reduced D. suzukii to the lowest level compared to any 1 species or 2 species combinations in a substitutive design. However, in caged plants with ripening fruit in a greenhouse, M. raptorellus and O. insidiosus together were more effective against D. suzukii than the 3 species combination. In high tunnels, M. raptorellus and O. insidiosus were ineffective against D. suzukii, and spinosad applications with or without M. raptorellus and O. insidiosus resulted in equally low numbers of D. suzukii. In the field, M. raptorellus and O. insidiosus and Pachycrepoideus vindemmiae Rondani (Hymenoptera: Pteromalidae) releases in raspberry plots with sweet alyssum were less effective against D. suzukii than spinosad applications to plots with or without sweet alyssum as a banker plant. Hymenoptera, particularly Figitidae: Eucoilinae as determined by barcoding, were more abundant in raspberries adjacent to sweet alyssum than in raspberry plots without sweet alyssum. With the discovery and redistribution of figitid parasitoids of larval D. suzukii, future research on their effects in combination with banker plants like sweet alyssum and releases of predators is needed.},
}

@article {pmid41131974,
year = {2025},
author = {Ben Ahmed, R and Gajda, Ł and Raś, D and Świątek, P},
title = {Taxonomic update and DNA barcoding of Tunisian nasal leeches (Annelida: Hirudiniformes; Praobdellidae): confirming Limnatis nilotica and revealing a second Limnatis species in North Africa.},
journal = {Invertebrate systematics},
volume = {39},
number = {4},
pages = {},
doi = {10.1071/IS24074},
pmid = {41131974},
issn = {1447-2600},
mesh = {Animals ; *DNA Barcoding, Taxonomic ; *Leeches/classification/genetics/anatomy & histology/ultrastructure ; Tunisia ; Species Specificity ; Phylogeny ; Africa, Northern ; Electron Transport Complex IV/genetics ; RNA, Ribosomal, 28S/genetics ; },
abstract = {Leeches of the genus Limnatis are known ectoparasites of vertebrates, including humans and domestic animals, with some species causing significant health complications. In this study, we describe Limnatis anouarensis sp. nov., a new nasal leech species from Tunisia and compare it with Limnatis nilotica sensu stricto from Tunisia and Morocco. Morphological analyses were conducted using light and scanning electron microscopy, focusing on external and internal structures. Additionally, mitochondrial (cytochrome c oxidase subunit I, COI; 12S rRNA, 12S) and nuclear (28S rRNA, 28S) gene fragments were sequenced for molecular characterisation. The new species is distinguished by its larger size, distinct reproductive system morphology and unique dorsal colouration, characterised by a median orange band and marginal orange stripes, in contrast to the black-striped pattern of L. nilotica (Moore, 1938). Molecular analyses confirmed that mitochondrial markers provide reliable species identification, whereas the analysed fragment of 28S gene was fully conserved and unsuitable for differentiation. These findings confirm the presence of at least two distinct Limnatis species in North Africa, emphasising the need for further taxonomic and ecological studies to clarify their distribution, host specificity and potential medical significance. ZooBank: urn:lsid:zoobank.org:pub:7CA59D0E-1D42-4A02-B886-D198DD88E698.},
}

Animals
*DNA Barcoding, Taxonomic
*Leeches/classification/genetics/anatomy & histology/ultrastructure
Tunisia
Species Specificity
Phylogeny
Africa, Northern
Electron Transport Complex IV/genetics
RNA, Ribosomal, 28S/genetics

@article {pmid41130498,
year = {2025},
author = {Said, MB},
title = {Advances in molecular taxonomy of Hyalomma ticks: from classical markers to next-generation omics.},
journal = {Acta tropica},
volume = {},
number = {},
pages = {107881},
doi = {10.1016/j.actatropica.2025.107881},
pmid = {41130498},
issn = {1873-6254},
abstract = {Ticks of the genus Hyalomma are important vectors of pathogens affecting humans and animals, including viruses, bacteria, and protozoans, and their expanding geographic range, driven by climate change and migratory birds, raises concerns about emerging disease outbreaks, specifically tick-borne diseases (TBDs) in previously unaffected regions. Despite this epidemiological significance, Hyalomma taxonomy remains challenging due to morphological variability and cryptic species complexes, particularly in immature stages. This review traces how molecular taxonomy has evolved from classical to next-generation approaches, emphasizing how successive methodological innovations have transformed species identification, phylogenetic reconstruction, and vector surveillance. Molecular systematics has greatly advanced species identification and phylogeographic understanding. For instance, mitochondrial markers enable reliable barcoding and reveal broad geographic patterns, while nuclear markers complement these insights and support functional genomics studies. Yet, earlier molecular tools have often fallen short in resolving closely related species or detecting fine-scale genetic differentiation essential for understanding vector competence and adaptation. More recently, high-throughput approaches such as population genomics, sialotranscriptomics, and MALDI-TOF MS have enhanced species discrimination and allowed rapid field-based identification. These next-generation omics platforms represent the new frontier of Hyalomma systematics, enabling comprehensive genetic, proteomic, and transcriptomic characterization that bridges taxonomy with function. However, despite these advances, significant challenges persist, including the scarcity of reference genomes for several Hyalomma species, limited integration of multi-omics datasets, and the lack of standardized bioinformatics pipelines that hinder data comparability across studies. Moreover, uneven geographic sampling and inconsistent marker selection continue to restrict our capacity to delineate cryptic lineages and link genetic diversity to epidemiological outcomes. By bridging taxonomy with function, these omics innovations establish an integrated framework linking genetic diversity to epidemiological risk and provide a roadmap for predictive, standardized, and applied Hyalomma taxonomy to improve vector surveillance and disease preparedness. This review provides the first comprehensive synthesis integrating classical, mitochondrial, nuclear, and multi-omics approaches for Hyalomma systematics.},
}

@article {pmid41127325,
year = {2025},
author = {Tong, Y and Liu, Z and Liu, H and Hou, Z},
title = {A new terrestrial talitrid genus, Kachinorchestia, with a new species from Myanmar (Crustacea, Amphipoda, Arcitalitridae).},
journal = {Biodiversity data journal},
volume = {13},
number = {},
pages = {e162403},
pmid = {41127325},
issn = {1314-2828},
abstract = {BACKGROUND: The Arcitalitridae (Amphipoda, Senticaudata, Talitrida, Talitroidea) is a diverse family containing 15 genera, of which Myanmarorchestia Hou, 2017 and Solitroides Suzuki, Nakano, Nguyen, Nguyen, Morino & Tomikawa, 2017 were reported in Southeast Asia. During research of terrestrial amphipods in Myanmar, we found character traits of some specimens did not match any existing genus of the family Arcitalitridae. These specimens should belong to a new genus.

NEW INFORMATION: This new genus Kachinorchestia Hou, gen. nov. with one new species Kachinorchestia putao Hou, sp. nov. is described from terrestrial habitats in Myanmar. This new genus is characterised by mandible left lacinia mobilis with four teeth, gnathopod II propodus of male enlarged, oval and subchelate, with hook posteriorly, simplidactylate pereopods, complex and lobed gills and telson uncleft. Photos, molecular and morphological descriptions of new genus are provided.},
}

@article {pmid41125033,
year = {2025},
author = {Rabbani, G and Chan, KH and Wainwright, BJ},
title = {Shark and ray meat sold for human consumption contains toxic metal concentrations above safe limits with concentrations varying by species and habitat.},
journal = {Marine pollution bulletin},
volume = {222},
number = {Pt 3},
pages = {118822},
doi = {10.1016/j.marpolbul.2025.118822},
pmid = {41125033},
issn = {1879-3363},
abstract = {Due to the high trophic positions sharks and rays occupy as apex predators, they are known to biomagnify toxic metals. This is problematic because shark meat consumption is increasing throughout the world where it is frequently seen as a cheap and accessible source of dietary protein. This increased consumption has the potential to expose humans to concentrations of toxic metals above those deemed safe by regulatory authorities. Shark and ray meat is frequently dried or sold as fillets making it difficult to identify the species. To overcome these identification challenges we used DNA barcoding to determine the species of shark used for meat. Two hundred and ninety-five samples were collected from retail establishments in Singapore and barcoded, this resulted in the identification of 17 species, 12 are predominantly inhabitants of coastal waters, while five are identified as oceanic species. Using inductively coupled plasma mass spectrometry the concentrations of mercury, arsenic, cadmium, and lead in each was determined. In all samples, across all species we found multiple instances where mandated safe concentrations for each metal were exceeded, with these concentrations differing significantly by species analysed. Further analysis, grouping samples by habitat type revealed significant differences in the concentrations of toxic metals between species from coastal and oceanic habitats. The results show that consumption of shark and ray meat can expose consumers to high concentrations of toxic metals, with these concentrations varying significantly by species and habitat. Given these differences, accurate and detailed product labelling that indicates the species of origin and habitat would allow consumers to avoid species that pose the highest risk to health.},
}

@article {pmid41124029,
year = {2025},
author = {Cunniffe, NJ and Gold, KM and Hamelin, FM and Navas-Cortés, JA and Potnis, N and Weisberg, AJ and Garrett, KA},
title = {From Chaos to Clarity: Deriving Meaningful Biology from Big Data in Plant Pathology.},
journal = {Phytopathology},
volume = {115},
number = {10},
pages = {1240-1244},
doi = {10.1094/PHYTO-10-25-0328-FI},
pmid = {41124029},
issn = {0031-949X},
mesh = {*Big Data ; *Plant Diseases ; *Plant Pathology ; *Plants ; },
abstract = {This Focus Issue was inspired by the vast volumes of data now being generated from both laboratory experiments and field studies. These complex, high dimensional data sets demand new analytic approaches. We invited contributions that apply novel statistical, machine learning, or artificial intelligence methods, or that integrate multiple 'omics approaches to reveal new aspects of pathogen biology, host interactions, or emergent system-level properties. The Focus Issue contains 13 articles falling into five broad themes and drawing upon a diverse range of data types, from disease imaging to meta-barcoding data to optical sensing to genetic data to expert knowledge. Together, these papers show how integrating technology, data, and biology is already transforming plant pathology. We hope this Focus Issue inspires new collaborations, new methods, and a continued commitment to turning data into understanding and chaos into clarity. [Formula: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 "No Rights Reserved" license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2025.},
}

*Big Data
*Plant Diseases
*Plant Pathology
*Plants

@article {pmid41122926,
year = {2025},
author = {Zhang, B and Jiang, W and Fang, Z and Chen, H and Jiang, N and Zhou, J and Wan, R and Li, S and Li, T and Cai, L and Song, H and Li, L and Gao, L and Chen, L and Peng, H},
title = {Development of a High-Resolution MNP Marker System for Aquatic Biodiversity Monitoring: A Case Study With Schizothorax prenanti in the Yangtze River.},
journal = {Molecular ecology resources},
volume = {},
number = {},
pages = {e70063},
doi = {10.1111/1755-0998.70063},
pmid = {41122926},
issn = {1755-0998},
support = {2021041-ZHX//Open Research Fund of Key Laboratory of Three Gorges Project for Conservation of Fishes/ ; 2023XKZ024//Excellent Discipline Cultivation Project by Jianghan University/ ; NBZZ20220230//The Research Projects of China Three Gorges Corporation/ ; 2024JCYJ06//The Research Fund of Jianghan University/ ; 2023010201020447//Wuhan Municipal Knowledge Innovation Special Project/ ; 2025AFB113//Natural Science Foundation of Hubei Province/ ; },
abstract = {Effective monitoring of aquatic biodiversity is critical for conservation, yet current approaches such as mitochondrial COI barcoding and microsatellite markers exhibit limitations in resolution, sensitivity, and scalability, particularly for detecting low-abundance or degraded DNA in mixed aquatic samples. To address these challenges, we developed a novel Multiple Nucleotide Polymorphism (MNP) marker system tailored to S. prenanti, an endangered endemic fish species emblematic of biodiversity crises in the Yangtze River. Through restriction-site associated DNA sequencing, we identified 115 genome-wide MNP markers. These markers demonstrated ultrasensitive detection (~1 DNA copy/reaction) and high specificity (mean discriminative power = 0.77, calculated as the probability that two random samples differ at a locus). When applied to environmental DNA from the Yangtze River, the MNP system revealed substantial genetic diversity among 86 samples (84% average differentiation rate) and quantified the contribution of artificially stocked fish to natural populations, identified 567 shared alleles between stocked and wild populations. By outperforming traditional methods in analysing fragmented DNA and enabling high-throughput applications, this MNP framework provides a transformative approach for conservation genetics. Our scalable solution bridges the gap between genetic research and conservation action, offering global applicability for aquatic biodiversity monitoring.},
}

@article {pmid41120480,
year = {2025},
author = {Johansen, NS and Eiken, HG and Rossmann, SL and Brurberg, MB and Skogen, M and Fajardo, MB and Glorvigen, B and Eklo, TS and Haugen, FA and Hagen, S and Lysøe, E},
title = {Aphid populations and virus vector potential in potato fields across seasons and regions in Norway.},
journal = {Scientific reports},
volume = {15},
number = {1},
pages = {36675},
pmid = {41120480},
issn = {2045-2322},
mesh = {*Aphids/virology/classification/genetics ; *Solanum tuberosum/virology/parasitology ; Animals ; Norway ; Seasons ; *Insect Vectors/virology ; *Plant Diseases/virology ; Potyvirus ; },
abstract = {Several aphid species pose serious treats to potato crops by causing direct damage to the plants and/or indirectly by transmitting viruses. Different morphological forms and phenotypic plasticity among aphids complicates taxonomy and identification and thus makes targeted pest management in potatoes challenging. To obtain an overview of aphids frequenting potato fields in Norway, we investigated seasonal and annual changes in aphid populations in five potato fields (58-64 °N) over a three-year period (2016-2018), using yellow pan traps. In total 2218 of the 6136 collected aphids were identified by traditional barcoding, meaning sequencing a ~ 650 fragment of the mitochondrial COI gene. This revealed 137 different species, of which 111 were identified at the species level. The remaining were identified only to the genus level, indicating potential novel species. The southernmost sampling location yielded the highest number of species and individual counts, although no clear correlations to climate factors (temperature/precipitation) was observed. Of the 111 species identified, at least 39 are potential vectors of potato virus Y (PVY) and nine species may also transmit potato virus A (PVA). Knowledge on virus vector and non-vector aphid abundance and phenology have the potential to improve pest management of potato cultivation.},
}

*Aphids/virology/classification/genetics
*Solanum tuberosum/virology/parasitology
Animals
Norway
Seasons
*Insect Vectors/virology
*Plant Diseases/virology
Potyvirus

@article {pmid41119951,
year = {2025},
author = {Geci, D and Ibrahimi, H and Strohmeier, T and Koblmller, S and Bilalli, A and Zamani, A},
title = {First DNA barcoding of Dysderidae (Araneae) from Kosovo, with new records and the description of a new species of Harpactea Bristowe, 1939.},
journal = {Zootaxa},
volume = {5653},
number = {4},
pages = {486-500},
doi = {10.11646/zootaxa.5653.4.2},
pmid = {41119951},
issn = {1175-5334},
mesh = {Animals ; *Spiders/classification/genetics/anatomy & histology/growth & development ; Male ; DNA Barcoding, Taxonomic ; Female ; Kosovo ; Animal Distribution ; Phylogeny ; Body Size ; Animal Structures/anatomy & histology/growth & development ; Organ Size ; },
abstract = {New taxonomic and faunistic data on the dysderid spiders of Kosovo are presented, along with a survey of previous records. Harpactea dardanica Geci & Zamani sp. nov., belonging to the lepida species-group, is described as new to science based on male specimens collected in the Bjeshkt-e-Nemuna National Park, western Kosovo. Dysdera adriatica Kulczyski, 1897, Dy. lata Reuss, 1834, Dasumia kusceri (Kratochvl, 1935), H. rubicunda (C.L. Koch, 1838), H. srednagora Dimitrov & Lazarov, 1999, and H. tenuiemboli Deltshev, 2011, as well as the genus Dasumia Thorell, 1875, are reported from Kosovo for the first time. Additional records are provided for Dy. longirostris Doblika, 1853 and H. lepida (C.L. Koch, 1838). DNA barcodes (partial sequences of the mitochondrial cytochrome c oxidase subunit 1 gene) were generated for all species. Photographic illustrations are provided for the newly described species, as well as for H. srednagora and H. tenuiemboli.},
}

Animals
*Spiders/classification/genetics/anatomy & histology/growth & development
Male
DNA Barcoding, Taxonomic
Female
Kosovo
Animal Distribution
Phylogeny
Body Size
Animal Structures/anatomy & histology/growth & development
Organ Size

@article {pmid41121673,
year = {2024},
author = {Dai, YT and Chen, ZG and Hu, CL and Ning, PF and Ouyang, S and Huang, XC and Wu, XP},
title = {Taxonomic reassessment of Scabies (Bivalvia: Unionidae) species in China based on multilocus and mitogenomic phylogenetic analyses.},
journal = {Invertebrate systematics},
volume = {38},
number = {6},
pages = {},
doi = {10.1071/IS24020},
pmid = {41121673},
issn = {1447-2600},
mesh = {Animals ; *Phylogeny ; China ; Species Specificity ; *Genome, Mitochondrial ; *Bivalvia/classification/genetics/anatomy & histology ; DNA Barcoding, Taxonomic ; },
abstract = {Effective species conservation necessitates the ability to accurately differentiate among species, a challenge compounded by taxonomic uncertainties in freshwater mussels due to substantial intraspecific variation and pronounced phenotypic plasticity in shell morphology. The taxonomic status and species validity of Scabies longata and S. chinensis, two species endemic in China, have been under continuous debate since establishment. The lack of essential molecular data required for a comprehensive systematic study has resulted in the unresolved taxonomic status of these two species. This study presents molecular data, including COI barcoding, COI + 28S rRNA, and mitogenomic data combined with morphological characteristics to assess the validity of S. longata and S. chinensis. Both morphological and COI barcoding data support the conclusion that S. longata and S. chinensis are junior synonyms of Nodularia douglasiae and N. nuxpersicae respectively. Our findings suggest the absence of Scabies species in China. Mitochondrial phylogenetic analyses were used to further elucidate intrageneric relationships within the genus Nodularia, revealing the following relationships: (N. breviconcha (Nodularia sp. 1 (N. douglasiae (N. nuxpersicae, N. nipponensis)))). We underscore the significance of employing an integrated taxonomic approach for species identification, especially given the considerable morphological disparities between larvae and adult freshwater mussels. Proper morphological identification of adult specimens is essential for extracting meaningful taxonomic characters. Furthermore, our findings suggest a notable resemblance between the freshwater bivalve fauna in southern China and those east of the Mekong River. ZooBank: urn:lsid:zoobank.org:pub:DA87D330-5E23-4F4B-8CC2-CBA3CD191BE8.},
}

Animals
*Phylogeny
China
Species Specificity
*Genome, Mitochondrial
*Bivalvia/classification/genetics/anatomy & histology
DNA Barcoding, Taxonomic

@article {pmid41119938,
year = {2025},
author = {Zheng, Y and Badano, D and Aspck, U and Aspck, H and Hayashi, F and Liu, X},
title = {Revision of the antlion genus Epacanthaclisis Okamoto, 1910 (Neuroptera: Myrmeleontidae: Dendroleontinae).},
journal = {Zootaxa},
volume = {5657},
number = {1},
pages = {1-100},
doi = {10.11646/zootaxa.5657.1.1},
pmid = {41119938},
issn = {1175-5334},
mesh = {Animals ; Male ; Female ; Animal Distribution ; *Neoptera/classification/anatomy & histology/growth & development ; Animal Structures/anatomy & histology/growth & development ; Body Size ; Organ Size ; Asia ; },
abstract = {The antlion genus Epacanthaclisis of the subfamily Dendroleontinae is herein revised. A total of 22 species (including 11 new species) are recorded from East to Central Asia and classified into four species groups: 1) the E. continentalis group: E. alaica Krivokhatsky, 1998, E. continentalis Esben-Petersen, 1935, E. darman Zheng, Badano, U. Aspck, H. Aspck & Liu sp. nov., E. hamata Krivokhatsky, 1998, E. kuldurguch Krivokhatsky, 1998, E. liuyingqiae Zheng, Badano, U. Aspck, H. Aspck & Liu sp. nov., E. victor Badano, Zheng & Liu sp. nov., E. zhiweii Zheng, Badano, U. Aspck, H. Aspck & Liu sp. nov., and E. zuqii Zheng, Badano, U. Aspck, H. Aspck & Liu sp. nov.; 2) the E. tuyuezhengi group: E. tuyuezhengi Zheng & Liu sp. nov., E. zhihaoi Zheng & Liu sp. nov.; 3) the E. maculosa group: E. jiazhii Zheng, U. Aspck, H. Aspck, Hayashi & Liu sp. nov., E. maculata (Yang, 1986), E. maculosa (Yang, 1986), E. minana (Yang, 1999), E. wuchaoi Zheng, Badano & Liu sp. nov., and E. xiaohongae Zheng & Liu sp. nov.; 4) the E. moiwana group: E. banksi Krivokhatsky, 1998, E. longwai Zheng, Hayashi & Liu sp. nov., E. moiwana (Okamoto, 1905), and E. ningshana Wan & Wang, 2010. Besides, E. batangana Yang, 1992, is not assigned to any species group. E. amydrovittata Wan & Wang, 2010 syn. nov. and E. samarkandica Krivokhatsky, 1998 syn. nov. are respectively synonymized as E. banksi and E. continentalis. Immature stages of five species (E. zuqii sp. nov., E. jiazhii sp. nov., E. minana, E. banksi, and E. ningshana) from China are reported after identification through rearing and COI barcoding. A key to the species of Epacanthaclisis is provided. The specialized morphological characters, systematic position, distribution pattern, and biology of Epacanthaclisis are discussed.},
}

Animals
Male
Female
Animal Distribution
*Neoptera/classification/anatomy & histology/growth & development
Animal Structures/anatomy & histology/growth & development
Body Size
Organ Size
Asia

@article {pmid41119906,
year = {2025},
author = {Pyrcz, TW and Boyer, P and Garlacz, R and Fhraeus, C and Andrade-C, MG and Blint, Z and Mahecha-J, O},
title = {Out of the blue: a new blue Lymanopoda butterfly from the pramo of northern Colombia raises questions about disjunct distributions (Lepidoptera: Nymphalidae, Satyrinae).},
journal = {Zootaxa},
volume = {5659},
number = {4},
pages = {547-564},
doi = {10.11646/zootaxa.5659.4.5},
pmid = {41119906},
issn = {1175-5334},
mesh = {Animals ; *Butterflies/classification/anatomy & histology/genetics/growth & development ; Male ; Colombia ; Female ; Animal Distribution ; Animal Structures/growth & development/anatomy & histology ; Body Size ; Ecosystem ; Organ Size ; Phylogeny ; },
abstract = {A new, striking species of satyrine butterfly, Lymanopoda chysquyco sp. nov. (Nymphalidae, Satyrinae, Satyrini, Pronophilina) is described from the Pramo de Guerrero, the northern extremity of high-altitude grasslands in the Colombian Eastern Cordillera. Among approximately 70 known Lymanopoda species, only five exhibit blue colour on the upperside of their wings, with no evidence of close relationships among them. The new species superficially resembles L. samius Westwood which occurs parapatrically at slightly lower elevations and belongs to a distantly related lineage within the genus. Remarkably, external morphology and male genitalia suggest a strong similarity of the new species to L. hazelana found in southern Ecuador and northernmost Peruover 1300 km away. Molecular analysis using COI barcoding confirms that L. hazelana and a few other closely related species also found in southern Ecuador are the closest known relatives of L. chysquyco sp. nov. This substantial geographic disjunction is unlikely to be a sampling artefact, as intermediate pramo regions of the Eastern and Central Colombian Cordillera and central Ecuador have been extensively surveyed. The origin of L. chysquyco sp. nov. in northern Colombia remains unclear, and two possible scenarioslong-distance dispersal or vicariance accompanied by large-scale extinction in intervening areasare considered.},
}

Animals
*Butterflies/classification/anatomy & histology/genetics/growth & development
Male
Colombia
Female
Animal Distribution
Animal Structures/growth & development/anatomy & histology
Body Size
Ecosystem
Organ Size
Phylogeny

@article {pmid41119904,
year = {2025},
author = {Li, SL and Zhou, YC and Liu, P and Liu, F and Peng, XJ},
title = {Three new species of the genus Draconarius Ovtchinnikov, 1999 (Araneae: Agelenidae) from Hunan Province, China.},
journal = {Zootaxa},
volume = {5659},
number = {4},
pages = {581-588},
doi = {10.11646/zootaxa.5659.4.7},
pmid = {41119904},
issn = {1175-5334},
mesh = {Male ; Animals ; China ; Female ; *Spiders/classification/anatomy & histology/genetics/growth & development ; Animal Distribution ; Animal Structures/anatomy & histology/growth & development ; Body Size ; Organ Size ; Phylogeny ; },
abstract = {Three new species of the genus Draconarius Ovtchinnikov, 1999 are described as such: D. circinatus sp. nov. (male, female), D. jiemuxiensis sp. nov. (female) and D. latus sp. nov. (male, female), from Hunan province, China. Detailed descriptions, photos of somatic features and copulatory organs, as well as the distribution map are provided. Nucleotide data for the barcoding gene, cytochrome c oxidase subunit I (COI) of D. circinatus sp. nov. (male, female) are provided.},
}

Male
Animals
China
Female
*Spiders/classification/anatomy & histology/genetics/growth & development
Animal Distribution
Animal Structures/anatomy & histology/growth & development
Body Size
Organ Size
Phylogeny

@article {pmid41119897,
year = {2025},
author = {Seong, JW and Park, JG and Nam, DH},
title = {Revisiting subspecies identification of Common Kestrels (Falco tinnunculus): A critical look at Zhang et al. (2008).},
journal = {Zootaxa},
volume = {5659},
number = {4},
pages = {536-546},
doi = {10.11646/zootaxa.5659.4.4},
pmid = {41119897},
issn = {1175-5334},
mesh = {Animals ; *Falconiformes/genetics/classification ; DNA, Mitochondrial/genetics ; Phylogeny ; China ; Male ; },
abstract = {Zhang et al. (2008) investigated subspecific variation of Common Kestrels (Falco tinnunculus) wintering in Beijing, China, using a partial mtDNA control region (394395bp), and concluded that two subspecies were present. However, analysis of their raw data revealed mostly double peaks at each nucleotide position, indicating ambiguity in the DNA sequence. These likely arose from a combination of factors, including irregular PCR efficiencies and slight primer mismatches. Zhang et al. (2008) likely misinterpreted these double peaks as evidence of distinct subspecies. This resulted in an inflated number (56) of variation sites. Their interpretation was further confounded by potential issues with their primers, designed from a sequence of F. peregrinus with a one-nucleotide difference. To address these limitations, we sequenced the full mtDNA control region (1,266bp) of 38 Common Kestrel samples using newly designed primers. This analysis yielded clean, single-peak profiles for each sequence, confirming that all samples belonged to F. t. interstinctus. We therefore propose reclassifying these populations as a single subspecies, F. t. interstinctus. Erroneous barcode sequences can distort subspecific identification and population structure, leading to inaccurate data and misleading phylogenetic inferences. Our study underscores the critical need for robust methods to minimize barcode sequencing errors. This would not only ensure accurate phylogenetic inferences for Common Kestrels but also support reliable population genetic studies across diverse taxa.},
}

Animals
*Falconiformes/genetics/classification
DNA, Mitochondrial/genetics
Phylogeny
China
Male

@article {pmid41119879,
year = {2025},
author = {Kamyab, F and Makhov, IA and Mokhtari, A},
title = {A new species of the genus Dzhugesia Wehrli (Lepidoptera: Geometridae, Ennominae) from Iran.},
journal = {Zootaxa},
volume = {5660},
number = {2},
pages = {255-266},
doi = {10.11646/zootaxa.5660.2.6},
pmid = {41119879},
issn = {1175-5334},
mesh = {Animals ; Iran ; *Moths/classification/anatomy & histology/growth & development/genetics ; Male ; Female ; Animal Distribution ; Organ Size ; Animal Structures/anatomy & histology/growth & development ; Body Size ; DNA Barcoding, Taxonomic ; },
abstract = {A series of geometrid moths belonging to the little-known genus Dzhugesia Wehrli, 1936, were collected in southern Iran (Kerman Province). Both morphological characters and DNA barcoding confirm that the specimens represent a new species, herein described as D. alierfani Makhov & Kamyab sp. nov. Additional remarks on the diagnostic characters of the genus Dzhugesia are provided. The relationships between Dzhugesia and the closely related genera Crocallis and Scodiomima are also discussed. Furthermore, the misidentification of a specimen previously submitted to the BOLD database is corrected. This study constitutes the first record of the genus Dzhugesia from Iran. D. miatleuskii is recorded as new species for the fauna of Iran.},
}

Animals
Iran
*Moths/classification/anatomy & histology/growth & development/genetics
Male
Female
Animal Distribution
Organ Size
Animal Structures/anatomy & histology/growth & development
Body Size
DNA Barcoding, Taxonomic

@article {pmid41119869,
year = {2025},
author = {Ruengsawang, N and Sangpradub, N and Cubeddu, T and Erpenbeck, D and Wrheide, G and Pronzato, R and Manconi, R},
title = {Bangkok urban census of aquatic invertebrate fauna by an integrative taxonomic approach: the case of Suan Luang Rama IX Park and its freshwater sponges (Spongillida: Spongillidae).},
journal = {Zootaxa},
volume = {5660},
number = {3},
pages = {371-388},
doi = {10.11646/zootaxa.5660.3.4},
pmid = {41119869},
issn = {1175-5334},
mesh = {Animals ; *Porifera/classification/anatomy & histology/genetics/growth & development ; Thailand ; Fresh Water ; Ecosystem ; Animal Distribution ; Phylogeny ; Animal Structures/growth & development/anatomy & histology ; Body Size ; },
abstract = {Faunistic investigations on freshwater bodies of the Bangkok Metropolitan Area resulted in the discovery of a freshwater sponge population fragmented in small lentic microhabitats at the Suan Luang Rama IX Park. Sponges living in the lotus and waterlily garden and tropical greenhouse were photographed in situ, and some representative, small samples were collected and stored at the Division of Biology (Porifera Collection), Rajamangala University of Technology Krungthep, Bangkok. The taxonomic integrative analyses focused on diagnostic morphotraits, barcoding, and biogeography. The sponge population was ascribed to Radiospongilla cf. cerebellata (Porifera: Demospongiae: Spongillida: Spongillidae) and is a new record for the Bangkok area and the entire Thai inland water. Comparative integrative analyses vs spongillid taxa worldwide resulted in the discovery of new gemmular morphotraits for the genus. Although the Thai Radiospongilla cf. cerebellata diverges from the Indian type of Radiospongilla cerebellata at the level of gemmular theca architecture, molecular analyses highlighted the matching of the former with the Radiospongilla cluster. A possible allochthonous origin of the Bangkok Radiospongillas population is hypothesised. This investigation on botanical gardens aquatic fauna and a review of appropriate literature highlights that sponges are able to inhabit very confined and fragmented, shallow water bodies, i.e., scattered terracotta pots, plastic containers, and small ponds with aquatic plants also in megalopolis gardens.},
}

Animals
*Porifera/classification/anatomy & histology/genetics/growth & development
Thailand
Fresh Water
Ecosystem
Animal Distribution
Phylogeny
Animal Structures/growth & development/anatomy & histology
Body Size

@article {pmid41119858,
year = {2025},
author = {Kawashima, I and Matsui, Y and Yagi, S},
title = {Taxonomic study of Japanese species of the genus Gibberifera Obraztsov (Lepidoptera: Tortricidae: Olethreutinae), with a description of a new species.},
journal = {Zootaxa},
volume = {5660},
number = {4},
pages = {547-559},
doi = {10.11646/zootaxa.5660.4.5},
pmid = {41119858},
issn = {1175-5334},
mesh = {Animals ; Female ; Male ; *Moths/anatomy & histology/classification/genetics/growth & development ; Japan ; Animal Distribution ; Body Size ; Organ Size ; Animal Structures/anatomy & histology/growth & development ; Phylogeny ; DNA Barcoding, Taxonomic ; },
abstract = {Gibberifera xylosmae sp. nov., whose larvae feed on Xylosma congesta (Salicaceae), is described in Japan. Photographs of adults, male and female genitalia of the three Japanese Gibberifera species are provided. The diagnosis, a key, and DNA barcodes of Japanese Gibberifera species are also presented. Intraspecific and interspecific genetic distances between Japanese Gibberifera species and other congeners are also provided.},
}

Animals
Female
Male
*Moths/anatomy & histology/classification/genetics/growth & development
Japan
Animal Distribution
Body Size
Organ Size
Animal Structures/anatomy & histology/growth & development
Phylogeny
DNA Barcoding, Taxonomic

@article {pmid41119853,
year = {2025},
author = {Mengual, X and Hauser, M},
title = {Atriadops Wandolleck, 1897 (Diptera: Nemestrinidae) recorded for the first time from Ecuador.},
journal = {Zootaxa},
volume = {5660},
number = {4},
pages = {596-600},
doi = {10.11646/zootaxa.5660.4.10},
pmid = {41119853},
issn = {1175-5334},
mesh = {Animals ; Ecuador ; Female ; *Diptera/classification/anatomy & histology/growth & development/genetics ; Animal Distribution ; Male ; Organ Size ; Body Size ; Animal Structures/anatomy & histology/growth & development ; Ecosystem ; DNA Barcoding, Taxonomic ; },
abstract = {The genus Atriadops Wandolleck, 1897 (Diptera: Nemestrinidae) occurs in the Neotropical, Afrotropical, Indomalayan and Australasian Regions. It is easily recognized by not having ocelli and the vestigial mouthparts. Out of the seven recognized species worldwide, only one is present in the Neotropics, A. macula (Wiedemann, 1824). We report the first record of Atriadops from Ecuador, a female of A. macula, from the Napo Province, provide the first DNA barcode for the species, and discuss the possible reasons for the low number of records of this genus.},
}

Animals
Ecuador
Female
*Diptera/classification/anatomy & histology/growth & development/genetics
Animal Distribution
Male
Organ Size
Body Size
Animal Structures/anatomy & histology/growth & development
Ecosystem
DNA Barcoding, Taxonomic

@article {pmid41119850,
year = {2025},
author = {Zheng, T and Wu, K and Zhang, F},
title = {First record of the subfamily Megaschizominae Rowland, 1973 from China, with description of a new species of Megaschizomus Lawrence, 1969 (Schizomida: Hubbardiidae).},
journal = {Zootaxa},
volume = {5661},
number = {1},
pages = {80-94},
doi = {10.11646/zootaxa.5661.1.3},
pmid = {41119850},
issn = {1175-5334},
mesh = {Animals ; Male ; China ; Female ; Animal Distribution ; Animal Structures/anatomy & histology/growth & development ; Organ Size ; Body Size ; },
abstract = {The subfamily Megaschizominae Rowland, 1973 is recorded from China for the first time through the description of a new species in the genus Megaschizomus Lawrence, 1969: M. zhongshanensis sp. nov. (male, female). This study presents the first illustrated description of male genital structures for a species within this subfamily. In addition to the detailed description, illustrations, diagnosis, and a distribution map are provided. Additionally, the DNA barcode of the new species is also provided.},
}

Animals
Male
China
Female
Animal Distribution
Animal Structures/anatomy & histology/growth & development
Organ Size
Body Size

@article {pmid41119838,
year = {2025},
author = {Huemer, P and Mayr, T and Nel, J and Stark, W},
title = {Epermenia reinhardgaedikei sp. nov., an overlooked species from central-southern Europe (Lepidoptera, Epermeniidae).},
journal = {Zootaxa},
volume = {5661},
number = {2},
pages = {237-248},
doi = {10.11646/zootaxa.5661.2.5},
pmid = {41119838},
issn = {1175-5334},
mesh = {Animals ; Male ; Female ; Animal Distribution ; *Moths/anatomy & histology/classification/growth & development/genetics ; Body Size ; Organ Size ; Europe ; Animal Structures/anatomy & histology/growth & development ; },
abstract = {Epermenia reinhardgaedikei sp. nov. is described from material collected in central Italy, south-eastern France, and eastern Austria. The species was hitherto mixed with the congeners E. theimeri Gaedike, 2001 and E. profugella (Stainton, 1856) due to superficial similarity in male genitalia. However, the new species is closest to E. iniquellus (Wocke, 1867), differing from that species and congeners in structures of male genitalia and by DNA barcodes. Adults, male and female genitalia are figured.},
}

Animals
Male
Female
Animal Distribution
*Moths/anatomy & histology/classification/growth & development/genetics
Body Size
Organ Size
Europe
Animal Structures/anatomy & histology/growth & development

@article {pmid41119791,
year = {2025},
author = {Lim, W and Bang, WJ and Shin, S},
title = {New records of Macrocera Meigen 1803 (Diptera, Keroplatidae) from the Korean Peninsula with description of a new species.},
journal = {Zootaxa},
volume = {5665},
number = {2},
pages = {239-255},
doi = {10.11646/zootaxa.5665.2.5},
pmid = {41119791},
issn = {1175-5334},
mesh = {Animals ; Republic of Korea ; Male ; Female ; *Diptera/classification/anatomy & histology/genetics/growth & development ; Animal Distribution ; Body Size ; Animal Structures/anatomy & histology/growth & development ; DNA Barcoding, Taxonomic ; Organ Size ; Phylogeny ; },
abstract = {A new speciesMacrocera interrupta sp. nov.is described from Wando, South Korea. We also report the first records of M. abdominalis Okada 1937 and M. maculosa Matsumura 1915 from South Korea. DNA barcoding data for all three species are presented. Their relationships with other Macrocera species from the Palearctic Region are briefly discussed.},
}

Animals
Republic of Korea
Male
Female
*Diptera/classification/anatomy & histology/genetics/growth & development
Animal Distribution
Body Size
Animal Structures/anatomy & histology/growth & development
DNA Barcoding, Taxonomic
Organ Size
Phylogeny

@article {pmid41119769,
year = {2025},
author = {Sohn, JC},
title = {A new species of Oruza Walker, 1861 (Lepidoptera, Erebidae, Boletobiinae) from Korea with barcode-based pairing.},
journal = {Zootaxa},
volume = {5665},
number = {4},
pages = {592-596},
doi = {10.11646/zootaxa.5665.4.8},
pmid = {41119769},
issn = {1175-5334},
mesh = {Animals ; Male ; Female ; Republic of Korea ; *Moths/classification/anatomy & histology/genetics/growth & development ; DNA Barcoding, Taxonomic ; Animal Distribution ; Animal Structures/anatomy & histology/growth & development ; Body Size ; Organ Size ; },
abstract = {A new species of the genus Oruza, O. koreana sp. nov. is described, based on 14 specimens collected in Korea. The species is closely related to Oruza obliquaria Marumo, 1932 but can be distinguished from the latter by the shapes of the costal process in the male genitalia and the lamella postvaginalis in the female genitalia. The occurrence of Oruza obliquaria in Korea is discussed. Two COI barcodes of O. koreana sp. nov. are provided to facilitate pairing and reliable identification.},
}

Animals
Male
Female
Republic of Korea
*Moths/classification/anatomy & histology/genetics/growth & development
DNA Barcoding, Taxonomic
Animal Distribution
Animal Structures/anatomy & histology/growth & development
Body Size
Organ Size

@article {pmid41119705,
year = {2025},
author = {Golovatch, SI and Enghoff, H and Efeykin, BD},
title = {Chondrodesmus riparius Carl, 1914, a millipede species new to the fauna of Costa Rica, originally described from Colombia, and introduced to and presently widespread across Europe (Diplopoda, Polydesmida, Chelodesmidae).},
journal = {Zootaxa},
volume = {5692},
number = {1},
pages = {161-174},
doi = {10.11646/zootaxa.5692.1.8},
pmid = {41119705},
issn = {1175-5334},
mesh = {Animals ; Costa Rica ; Male ; Female ; Colombia ; *Arthropods/classification/anatomy & histology/growth & development/genetics ; Europe ; Animal Distribution ; Animal Structures/anatomy & histology/growth & development ; Body Size ; Organ Size ; Introduced Species ; Ecosystem ; },
abstract = {The fairly large-bodied Neotropical millipede genus Chondrodesmus Silvestri, 1897, includes 23 species described from Central America, to Costa Rica in the north, and 25 more from South America, to Colombia, Venezuela, Peru and central-western Amazonia of Brazil in the south. Among them, seven species have hitherto been described or recorded from Costa Rica, including Chondrodesmus hoffmanni (Peters, 1865), from an unknown place, and here revised based on the holotype. Unexpectedly, it appears to differ markedly from all Costa Rican congeners, and instead it shows profound similarities to Chondrodesmus riparius Carl, 1914, from Colombia. The status of the European introduction heretofore provisionally referred to either as C. cf. riparius or C. riparius is confirmed here, since morphologically the European and Tropical American C. riparius populations represent the same species. To support this, comparative molecular studies using COI barcoding data, freshly obtained from a population from Costa Rica, with a European population of C. riparius show a congruence of 99.2%. This not only indicates the conspecificity of C. riparius from South America and Europe, but it also suggests the source area whence its introduction to Europe could have occurred.},
}

Animals
Costa Rica
Male
Female
Colombia
*Arthropods/classification/anatomy & histology/growth & development/genetics
Europe
Animal Distribution
Animal Structures/anatomy & histology/growth & development
Body Size
Organ Size
Introduced Species
Ecosystem

@article {pmid41119695,
year = {2025},
author = {Semenchenko, AA and Palatov, DM and Makarchenko, EA},
title = {A review of the taxonomy, distribution and population genetic analysis of the Diamesa cinerella group (Diptera: Chironomidae: Diamesinae), with a description of D. soktoshensis sp. nov. and DNA barcoding of known species.},
journal = {Zootaxa},
volume = {5692},
number = {1},
pages = {121-147},
doi = {10.11646/zootaxa.5692.1.6},
pmid = {41119695},
issn = {1175-5334},
mesh = {Animals ; Male ; DNA Barcoding, Taxonomic ; Animal Distribution ; *Chironomidae/classification/genetics/anatomy & histology/growth & development ; Phylogeny ; Female ; Body Size ; Organ Size ; Animal Structures/growth & development/anatomy & histology ; },
abstract = {We provide original identification keys for adult males, brief redescriptions of the seven known species, a description of a new species, D. soktoshensis sp. nov., and a molecular genetic analysis of species of the Diamesa cinerella group. Population genetic analysis of Diamesa cinerella group revealed 109 haplotypes for 183 samples and a high level of haplotype diversity0.984 0.004. We identified four haplogroups, including Diamesa tsutsuii (1), Diamesa lavillei (2), Diamesa. soktoshensis sp. nov. (3) and the remaining species (4). The four haplogroups were significantly different from each other by FST values (P < 0.01). We explored the use of the internal transcribed spacers (ITS) region as an alternative nuclear DNA barcode forspecies delimitation of the Diamesa cinerella group. Three haplogroups were identified with no obvious connection to the species or sampling location.},
}

Animals
Male
DNA Barcoding, Taxonomic
Animal Distribution
*Chironomidae/classification/genetics/anatomy & histology/growth & development
Phylogeny
Female
Body Size
Organ Size
Animal Structures/growth & development/anatomy & histology

@article {pmid41119691,
year = {2025},
author = {Zhang, H and Li, Q and Yang, Z and Zhang, F},
title = {Description of five new species of Zodariidae Thorell, 1881 (Araneae) from Yunnan, China.},
journal = {Zootaxa},
volume = {5692},
number = {2},
pages = {256-276},
doi = {10.11646/zootaxa.5692.2.3},
pmid = {41119691},
issn = {1175-5334},
mesh = {Animals ; *Spiders/classification/anatomy & histology/genetics/growth & development ; China ; Male ; Female ; Animal Distribution ; Animal Structures/anatomy & histology/growth & development ; Body Size ; Organ Size ; },
abstract = {Five new ant-eating spiders from Yunnan, China, are described: Asceua banlaoensis sp. nov. (), A. nangunhe sp. nov. (), Euryeidon cervicornis sp. nov. (), Mallinella acutidentata sp. nov. () and M. tongbiguan sp. nov. (); the habitus and copulatory organs are documented, and a distribution map is provided for all new species. The association between males and females of Euryeidon cervicornis sp. nov. was confirmed by genetic similarity (COI barcoding).},
}

Animals
*Spiders/classification/anatomy & histology/genetics/growth & development
China
Male
Female
Animal Distribution
Animal Structures/anatomy & histology/growth & development
Body Size
Organ Size

@article {pmid41119662,
year = {2025},
author = {Schmidt, BC and Gartshore, M},
title = {Ecology, morphology and distribution of Anisota finlaysoni Riotte (Lepidoptera, Saturniidae).},
journal = {Zootaxa},
volume = {5646},
number = {1},
pages = {63-77},
doi = {10.11646/zootaxa.5646.1.3},
pmid = {41119662},
issn = {1175-5334},
mesh = {Animals ; Animal Distribution ; Male ; *Moths/anatomy & histology/classification/growth & development/genetics ; Female ; Ecosystem ; Larva/anatomy & histology/classification/growth & development/genetics ; Ontario ; Body Size ; Animal Structures/growth & development/anatomy & histology ; Organ Size ; DNA Barcoding, Taxonomic ; },
abstract = {Identifying the conservation needs of the short-horned oakworm, Anisota finlaysoni Riotte, has been hampered by incomplete resolution of species boundaries and geographic ranges of the Anisota senatoria species-group. Here, the life history, morphology, occurrence, and DNA barcode variation of A. finlaysoni and A. senatoria are compared across a transect of three key geographic regions in Ontario, Canada. Contrary to recent range depictions, our results show that A. finlaysoni is currently confirmed to occur only in the region of eastern Lake Ontarios north shore. Previous information on morphological characters used to distinguish A. finlaysoni is incomplete or erroneous. Lake Erie populations most recently assigned to A. finlaysoni exhibit some finlaysoni-like larval traits, but adult phenotype of all examined material is indistinguishable from A. senatoria of the Lake Huron region, and unlike topotypical A. finlaysoni. Although the cryptic occurrence of A. finlaysoni in the Lake Erie region cannot be ruled out, current data indicate that this species has a much smaller global range than previously believed, and is separated by a geographic gap of about 200 km from the nearest A. senatoria populations. Re-assignment of the Lake Erie populations from A. finlaysoni to A. senatoria, and the recognition of some peripheral occurrence records as historical errors, results in a significantly smaller global range for A. finlaysoni. DNA barcode data for the genus Anisota are at odds with current taxonomy, limiting the utility of barcodes in discriminating species of the senatoria-group. Because A. finlaysoni is distinct morphologically as adults and larvae from geographically nearby populations of A. senatoria, it should continue to be treated as a distinct species pending convincing evidence to the contrary.},
}

Animals
Animal Distribution
Male
*Moths/anatomy & histology/classification/growth & development/genetics
Female
Ecosystem
Larva/anatomy & histology/classification/growth & development/genetics
Ontario
Body Size
Animal Structures/growth & development/anatomy & histology
Organ Size
DNA Barcoding, Taxonomic

@article {pmid41119576,
year = {2025},
author = {Debnath, R and Reddy, BT and Rajmohana, K and Dinesh, KP},
title = {Morphological and molecular characterization of a new species of Hadronotus Frster (Hymenoptera: Scelionidae) reared from reduviid eggs in India.},
journal = {Zootaxa},
volume = {5711},
number = {3},
pages = {424-434},
doi = {10.11646/zootaxa.5711.3.7},
pmid = {41119576},
issn = {1175-5334},
mesh = {Animals ; India ; Female ; Phylogeny ; Male ; Animal Distribution ; *Reduviidae/parasitology ; *Wasps/anatomy & histology/classification/genetics/growth & development ; Organ Size ; Body Size ; Animal Structures/anatomy & histology/growth & development ; Electron Transport Complex IV/genetics ; DNA Barcoding, Taxonomic ; Ovum/parasitology ; },
abstract = {Hadronotus omnistriatus Debnath, Rajmohana and Thirupam Reddy sp. nov. (Hymenoptera: Scelionidae) is described herein as new to science from India under the muscaeformis species group. The specimens were reared from eggs of a reduviid host (Hemiptera: Reduviidae). A detailed morphological description is provided, along with its DNA barcode data. A single gene-based molecular phylogenetic analysis on the mitochondrial cytochrome c oxidase I is presented to support the delineation of the new species. Additionally, a checklist of Hadronotus species reported from India is provided.},
}

Animals
India
Female
Phylogeny
Male
Animal Distribution
*Reduviidae/parasitology
*Wasps/anatomy & histology/classification/genetics/growth & development
Organ Size
Body Size
Animal Structures/anatomy & histology/growth & development
Electron Transport Complex IV/genetics
DNA Barcoding, Taxonomic
Ovum/parasitology

@article {pmid41119552,
year = {2025},
author = {Timossi, G},
title = {Micropterix vulcanica sp. nov. (Lepidoptera, Micropterigidae) discovered on the island of Pantelleria (Italy, Sicily).},
journal = {Zootaxa},
volume = {5683},
number = {2},
pages = {282-288},
doi = {10.11646/zootaxa.5683.2.7},
pmid = {41119552},
issn = {1175-5334},
mesh = {Animals ; Sicily ; Male ; Female ; Animal Distribution ; Body Size ; Organ Size ; Animal Structures/anatomy & histology/growth & development ; DNA Barcoding, Taxonomic ; *Moths/anatomy & histology/classification/genetics/growth & development ; Islands ; Ecosystem ; *Butterflies/anatomy & histology/classification/genetics/growth & development ; Italy ; },
abstract = {Micropterix vulcanica sp. nov. is described, found during research on the biodiversity of Lepidoptera on Pantelleria Island National Park (Italy, Sicily). The new species has a unique combination of color pattern of the wings, genital morphology and DNA barcode.},
}

Animals
Sicily
Male
Female
Animal Distribution
Body Size
Organ Size
Animal Structures/anatomy & histology/growth & development
DNA Barcoding, Taxonomic
*Moths/anatomy & histology/classification/genetics/growth & development
Islands
Ecosystem
*Butterflies/anatomy & histology/classification/genetics/growth & development
Italy

@article {pmid41119529,
year = {2025},
author = {Lvarez, Y and Nez, R and Magaldi, LM and Matthews, D and Freitas, AVL and Espeland, M},
title = {Phenotypes, natural history and barcodes unveil cryptic species within the Caribbean Metalmark Dianesia carteri (Holland) (Lepidoptera: Riodinidae).},
journal = {Zootaxa},
volume = {5686},
number = {1},
pages = {5-48},
doi = {10.11646/zootaxa.5686.1.2},
pmid = {41119529},
issn = {1175-5334},
mesh = {Animals ; *Butterflies/classification/anatomy & histology/genetics/growth & development ; DNA Barcoding, Taxonomic ; Female ; Male ; Phylogeny ; Animal Distribution ; Body Size ; Organ Size ; Caribbean Region ; Animal Structures/growth & development/anatomy & histology ; Phenotype ; Ecosystem ; },
abstract = {The butterfly genus Dianesia Harvey & Clench, 1980 is the only known representative of the family Riodinidae in the West Indies with a single described species containing two subspecies, represented by rare, little-known populations occurring only in the Bahamas and Cuba. Until now, the genus has been regarded as monotypic, but differences in morphology, DNA barcodes and life history suggest that it contains multiple cryptic species. Our assessment led to recognize the existence of at least nine species within Dianesia: Dianesia carteri (Holland, 1902), Dianesia ramsdeni (Skinner, 1912) (previously regarded as a subspecies of the first), Dianesia galindoensis Barro, Hernndez & Torres, 2025, and the newly herein described Dianesia aberrans sp. nov., Dianesia sheylae sp. nov., Dianesia alayoi sp. nov., Dianesia flammata sp. nov., Dianesia abscondita sp. nov. and Dianesia serpentinicola sp. nov. These species remained unnoticed due to their superficial resemblance and lack of information about their biology, but can be differentiated by a combination of their DNA barcodes, wing length and shape, and elements of the color pattern, particularly the forewing postdiscal white band. Genitalia, habitat, host plant, and larval morphology also serve to differentiate the species. Bayesian and maximum likelihood COI gene trees recovered a similar topology in which all species are reciprocally monophyletic except for D. alayoi nested inside D. flammata. Species delimitation analyses supported the described species, including those that are not monophyletic, and suggested the presence of three additional species, but we regard them as artifacts produced by specimens with slightly different barcodes. Future research employing more genetic and ecological data is necessary to clarify the relationships among these species, as well as to understand their biogeographical history, ecology, and behavior, and to provide baseline knowledge for their conservation.},
}

Animals
*Butterflies/classification/anatomy & histology/genetics/growth & development
DNA Barcoding, Taxonomic
Female
Male
Phylogeny
Animal Distribution
Body Size
Organ Size
Caribbean Region
Animal Structures/growth & development/anatomy & histology
Phenotype
Ecosystem

@article {pmid41119491,
year = {2025},
author = {Zhou, YC and Qin, XY and Zhou, GC and Peng, XJ and Liu, P},
title = {Two new species of the genus Tmarus Simon, 1875 (Araneae: Thomisidae) from China.},
journal = {Zootaxa},
volume = {5666},
number = {1},
pages = {105-114},
doi = {10.11646/zootaxa.5666.1.5},
pmid = {41119491},
issn = {1175-5334},
mesh = {Animals ; China ; Male ; Female ; *Spiders/classification/anatomy & histology/genetics/growth & development ; Animal Distribution ; Animal Structures/anatomy & histology/growth & development ; Organ Size ; Body Size ; Phylogeny ; },
abstract = {Two new species of the genus Tmarus Simon, 1875 are described and named as T. yueluensis sp. nov. () and T. rostratus sp. nov. () from Hunan Province. Detailed description, photographs of somatic features and copulatory organs, as well as a distribution map are provided. Nucleotide data for the barcoding gene, cytochrome c oxidase subunit I (COI) of T. yueluensis. sp. nov. () are provided.},
}

Animals
China
Male
Female
*Spiders/classification/anatomy & histology/genetics/growth & development
Animal Distribution
Animal Structures/anatomy & histology/growth & development
Organ Size
Body Size
Phylogeny

@article {pmid41119488,
year = {2025},
author = {Garzn-Ordua, IJ and Brower, AVZ},
title = {A new cryptic Phyllodonta Warren (Lepidoptera: Geometridae) from Mexico City with documentation of its life history.},
journal = {Zootaxa},
volume = {5666},
number = {1},
pages = {136-144},
doi = {10.11646/zootaxa.5666.1.8},
pmid = {41119488},
issn = {1175-5334},
mesh = {Animals ; Mexico ; Female ; *Moths/anatomy & histology/classification/growth & development/genetics ; Male ; Animal Distribution ; Larva/anatomy & histology/classification/growth & development ; Body Size ; Organ Size ; Animal Structures/anatomy & histology/growth & development ; Ecosystem ; },
abstract = {A new species of Phyllodonta is described and illustrated from Mexico: P. coztomatlivora sp. nov. The new species is superficially indistinguishable from others in the so-called latrata species group, from which three other cryptic species from Costa Rica were recently described. However, it differs from them in features of the female genitalia, caterpillar morphology, larval food plant choice and DNA barcode. The new entity may be restricted to high elevations of the Trans Mexican Volcanic Belt; in Mexico City, the species is found inside a protected natural area which should warrant some level of conservation.},
}

Animals
Mexico
Female
*Moths/anatomy & histology/classification/growth & development/genetics
Male
Animal Distribution
Larva/anatomy & histology/classification/growth & development
Body Size
Organ Size
Animal Structures/anatomy & histology/growth & development
Ecosystem

@article {pmid41119451,
year = {2025},
author = {Jaume-Schinkel, S},
title = {Morpho-Molecular species delimitation within Tonnoira Enderlein (Diptera, Psychodidae): Updates on COI barcode data and the description of five new species.},
journal = {Zootaxa},
volume = {5673},
number = {1},
pages = {1-26},
doi = {10.11646/zootaxa.5673.1.1},
pmid = {41119451},
issn = {1175-5334},
mesh = {Animals ; DNA Barcoding, Taxonomic ; Male ; Female ; Animal Distribution ; Panama ; *Psychodidae/classification/anatomy & histology/genetics/growth & development ; Electron Transport Complex IV/genetics ; Animal Structures/anatomy & histology/growth & development ; Body Size ; Organ Size ; },
abstract = {This work describes five new species of Tonnoira Enderlein, namely, T. acantha sp. nov., T. asymmetrica sp. nov., T. sinuosa sp. nov., T. stria sp. nov., and T. wachi sp. nov., increasing the total number of species in this genus to 33. Additionally, T. conistylus Jaume-Schinkel, 2022 is documented for the first time in Panama. COI barcodes are provided for four of these newly described species and for T. fusiformis Quate & Brown, 2004. Molecular species delimitation analyses demonstrate the effectiveness of COI barcodes in identifying and discovering Psychodidae species in the Neotropics. This work highlights the current state of COI barcoding for Tonnoira species, emphasizing the importance of integrating molecular tools with traditional taxonomy to enhance species identification and conservation strategies. These findings contribute to a more comprehensive understanding of species diversity within this genus and support ongoing biodiversity conservation efforts.},
}

Animals
DNA Barcoding, Taxonomic
Male
Female
Animal Distribution
Panama
*Psychodidae/classification/anatomy & histology/genetics/growth & development
Electron Transport Complex IV/genetics
Animal Structures/anatomy & histology/growth & development
Body Size
Organ Size

@article {pmid41119442,
year = {2025},
author = {Hoang, QD and Truong, BP and Tran, TTT},
title = {ReclassificationofCoelotes songae(Araneae:Agelenidae:Coelotinae)withdescriptionofitsunknownfemaleconfirmedbymorphologyandDNAbarcoding.},
journal = {Zootaxa},
volume = {5673},
number = {1},
pages = {143-150},
doi = {10.11646/zootaxa.5673.1.10},
pmid = {41119442},
issn = {1175-5334},
mesh = {Animals ; Female ; *Spiders/classification/anatomy & histology/genetics/growth & development ; Male ; Animal Distribution ; Vietnam ; Animal Structures/anatomy & histology/growth & development ; Organ Size ; Body Size ; DNA Barcoding, Taxonomic ; },
abstract = {The genus Sinocoelotes Zhao & Li, 2016 is recorded from Vietnam for the first time based on the reclassificationof Coelotes songae Liu, Li & Pham, 2010 as Sinocoelotes songae (Liu, Li & Pham, 2010) comb. nov. The previously unknown female is described for the first time from type locality, as confirmed through DNA barcoding and morphological analysis.},
}

Animals
Female
*Spiders/classification/anatomy & histology/genetics/growth & development
Male
Animal Distribution
Vietnam
Animal Structures/anatomy & histology/growth & development
Organ Size
Body Size
DNA Barcoding, Taxonomic

@article {pmid41119438,
year = {2025},
author = {Beljaev, VA and Gorbunov, PY and Makhov, IA},
title = {An enigmatic new species of the genus Catarhoe (Lepidoptera: Geometridae: Larentiinae) from Kyrgyzstan and taxonomic notes to the genus.},
journal = {Zootaxa},
volume = {5618},
number = {3},
pages = {372-392},
doi = {10.11646/zootaxa.5618.3.4},
pmid = {41119438},
issn = {1175-5334},
mesh = {Animals ; Male ; *Moths/classification/anatomy & histology/genetics/growth & development ; Kyrgyzstan ; Female ; Animal Distribution ; Body Size ; Organ Size ; Animal Structures/growth & development/anatomy & histology ; Phylogeny ; DNA Barcoding, Taxonomic ; },
abstract = {The paper presents a new species of geometrid moth of the genus Catarhoe Herbulot, 1951 from Kyrgyzstan, and offers a concise taxonomic review of the genus based on existing literature and on an analysis of COI barcode mitochondrial DNA fragment in Epirrhoini. A new species and new monotypic subgenus are described: Catarhoe (Hyporhoe subgen. nov.) narynensis sp. nov. The species is distinguished by a unique set of characters in the male genitalia, which markedly differs from those of other congeneric species, and has significant genetic distances from the rest of the Catarhoe spp. but not exceeding genetic distances into the genus at whole. The current species composition of the genus Catarhoe has been clarified and includes 13 species. Taxonomic status of Catarhoe nyctichroa (Hampson, 1912), Catarhoe arachne hissarica Viidalepp, 1988, and Catarhoe semnana sensu Kemal et al. (2020) requires clarification. A high level of morphological and genetic diversity of the genus Catarhoe was revealed. The genetic distances between the morphological groups of Catarhoe spp. are comparable to those between the genera of Epirrhoini. The generic name Microcalcarifera Inoue, 1982, is revived from its synonymy with Catarhoe. The genus includes type species Microcalcarifera obscura (Butler, 1878: 450) (Cidaria), comb. rev., and two subspecies: Microcalcarifera obscura fecunda (Swinhoe, 1891), comb. rev. and Microcalcarifera obscura multilinea (Hampson, 1891), comb. rev.},
}

Animals
Male
*Moths/classification/anatomy & histology/genetics/growth & development
Kyrgyzstan
Female
Animal Distribution
Body Size
Organ Size
Animal Structures/growth & development/anatomy & histology
Phylogeny
DNA Barcoding, Taxonomic

@article {pmid41119392,
year = {2025},
author = {Kosterin, OE and Vierstraete, A and Schneider, T and Kompier, T and Hu, FS and Jr, LE and Makbun, N and Onishko, VV and Papazian, M and Dumont, HJ},
title = {Molecular phylogenetic analysis of the family Macromiidae (Odonata) worldwide based on a mitochondrial and two nuclear markers, with a short overview of its taxonomic history.},
journal = {Zootaxa},
volume = {5620},
number = {4},
pages = {501-545},
doi = {10.11646/zootaxa.5620.4.1},
pmid = {41119392},
issn = {1175-5334},
mesh = {Animals ; *Phylogeny ; Female ; Male ; Animal Distribution ; },
abstract = {Three molecular markers (two traditional and a new one), the barcoding fragment of the mitochondrial COI gene, the nuclear ITS region of the nucleolus organiser, and the nuclear histone H3H4 region including partial sequences of the highly conserved genes of core histones H3 and H4 and the non-coding spacer between them, were sequenced in 38 (31% of all) species of Macromiidae, representing all its four genera. Besides, 15 species of the related incertae sedis genera Macromidia Martin, 1907, Idionyx Hagen, 1867 and Oxygastra Selys, 1870 were sequenced. Available sequences of the concerned groups were also adopted from GenBank. All markers resolved Macromiidae as a monophyletic clade with the highest support. In contrast, our markers did not resolve the expected monophyletic branch containing the incertae sedis genera. Didymops transversa (Say, 1840) clustered with Macromia Rambur, 1842 in most trees as an inner lineage in the Macromia cluster. For this reason, we restored the synonymy of Didymops Rambur, 1842 with Macromia. Epophthalmia Burmeister, 1839 and Phyllomacromia Selys, 1878 were resolved as sister branches, as proposed before on morphological basis. On the species level, Macromia fraenata Martin, 1907, Macromia clio Ris, 1916 and Macromia kubokaiya Asahina, 1964 were restored as valid species names. Macromia flavocolorata Fraser, 1922 was downgraded to the subspecies Macromia calliope flavocolorata, stat rev., but the name Macromia miniata Fraser, 1924 was restored as valid species name to denote the species of the Western Ghats of India once considered as M. flavocolorata as well. The synonymy of M. cupricincta Fraser, 1924 and M. berlandi Lieftinck, 1941 at the species level were confirmed but the latter was treated as M. cupricincta berlandi stat. rev. Macromia hamata Zhou, 2003 was synonymised to Macromia manchurica Asahina, 1964. The presence of Epophthalmia vittata Burmeister, 1839 in Indochina as a common species was confirmed. The situation with Macromia callisto Laidlaw, 1922 remains obscure.},
}

Animals
*Phylogeny
Female
Male
Animal Distribution

@article {pmid41119380,
year = {2025},
author = {Can, L and Aydemr, HB},
title = {New records with DNA barcodes of the family Proctotrupidae (Hymenoptera: Proctotrupoidea) from Trkiye.},
journal = {Zootaxa},
volume = {5621},
number = {1},
pages = {131-143},
doi = {10.11646/zootaxa.5621.1.6},
pmid = {41119380},
issn = {1175-5334},
mesh = {Animals ; DNA Barcoding, Taxonomic ; Female ; Male ; Animal Distribution ; Phylogeny ; Body Size ; Turkey ; Organ Size ; Animal Structures/growth & development/anatomy & histology ; Electron Transport Complex IV/genetics ; *Wasps/classification/genetics/anatomy & histology/growth & development ; },
abstract = {The current study presents faunistic data concerning the Proctotrupidae family from the Central Black Sea region, specifically the Yeilrmak Delta in Samsun Province, Trkiye. Specimens were collected using Malaise traps over a period from March to September 2023. A total of five species across five genera of Proctotrupidae were identified. Notably, the genera Codrus Panzer, 1805, Cryptoserphus Kieer, 1907, and Mischoserphus Townes, 1981, along with the species Codrus picicornis Foerster, 1856, Cryptoserphus aculeator (Haliday, 1839) and Mischoserphus obesus Townes, 1981 are reported for the first time in Trkiye. An identification key for the existing genera of Turkish Proctotrupidae is included. The study also investigated the use of mitochondrial cytochrome oxidase subunit 1 (COI) gene-based DNA barcoding for species identification. Partial sequence data for Proctotrupidae species Cryptoserphus aculeator, Exallonyx crenicornis, Mischoserphus obesus, Phaneroserphus calcar, and Phaenoserphus viator were obtained and submitted to the NCBI database under accession numbers PQ423217PQ423221.The COI barcode sequences for M. obesus, which were previously undocumented in databases, are also presented. A phylogenetic tree constructed from COI sequences facilitates the identification and comparison of taxa. Despite observable morphological differences, certain species were found to be closely related, indicating the necessity for further research encompassing a wider array of species and specimens. The findings suggest that COI barcoding serves as an effective tool for the accurate and expedient identification of Proctotrupidae species.},
}

Animals
DNA Barcoding, Taxonomic
Female
Male
Animal Distribution
Phylogeny
Body Size
Turkey
Organ Size
Animal Structures/growth & development/anatomy & histology
Electron Transport Complex IV/genetics
*Wasps/classification/genetics/anatomy & histology/growth & development

@article {pmid41119377,
year = {2025},
author = {Oliveira-Filho, RR and Antunes, M and Tamburus, AF and Oliveira-Rogeri, L and Bochini, GL and Hostim-Silva, M and Mantelatto, FL},
title = {New records of decapod crustaceans (Crustacea, Decapoda) in the central coast of Brazil supported by integrative analysis.},
journal = {Zootaxa},
volume = {5621},
number = {2},
pages = {196-210},
doi = {10.11646/zootaxa.5621.2.2},
pmid = {41119377},
issn = {1175-5334},
mesh = {Animals ; Brazil ; *Decapoda/classification/anatomy & histology/growth & development/genetics ; Male ; Animal Distribution ; Female ; Body Size ; Animal Structures/anatomy & histology/growth & development ; Ecosystem ; Organ Size ; },
abstract = {The decapod fauna of the coast of Esprito Santo, southeastern Brazil, is poorly studied. Herein we report for the first time, for the region, the occurrence of eight species of crustaceans, representing an important contribution to the knowledge about the Brazilian biodiversity. The specimens were collected near the mouths of four estuaries (So Mateus, Ipiranga, Doce and Piraqu-A). For identification of the newly recorded species, Apiomithrax violaceus (A. Milne-Edwards, 1867), Notolopas brasiliensis Miers, 1886, Speloephorus elevatus Rathbun, 1898, Acantholobulus caribbaeus (Stimpson, 1871), Hexapanopeus angustifrons (Benedict & Rathbun, 1891), Hexapanopeus paulensis Rathbun, 1930, Mesorhoea sexspinosa Stimpson, 1871 and Leander paulensis Ortmann, 1897, morphology was assessed using specific literature. DNA barcoding was used as a complementary tool for the species identification. The present study fills many gaps in the geographic distribution of crustaceans in the Western Atlantic and will serve as an updated record and baseline for future studies along the Brazilian coast.},
}

Animals
Brazil
*Decapoda/classification/anatomy & histology/growth & development/genetics
Male
Animal Distribution
Female
Body Size
Animal Structures/anatomy & histology/growth & development
Ecosystem
Organ Size

@article {pmid41119374,
year = {2025},
author = {Timossi, G and Huemer, P},
title = {Adela paludicolella Zeller, 1850, and Adela orientella Staudinger, 1870 sp. rev. (Lepidoptera, Adelidae): two distinct species revealed by morphological analysis and DNA barcoding.},
journal = {Zootaxa},
volume = {5621},
number = {2},
pages = {249-261},
doi = {10.11646/zootaxa.5621.2.5},
pmid = {41119374},
issn = {1175-5334},
mesh = {Animals ; DNA Barcoding, Taxonomic ; Male ; Female ; Animal Distribution ; Organ Size ; *Moths/anatomy & histology/classification/genetics/growth & development ; Animal Structures/anatomy & histology/growth & development ; Body Size ; Phylogeny ; Greece ; Italy ; },
abstract = {The morphological study of A. paludicolella specimens collected in Italyspecifically from the island of Elba (Tuscany) and Sardiniaas well as from Greece, has revealed differences in habitus and reproductive organs between the Italian and Greek specimens. Subsequent genetic analysis confirmed the genetic distinction between A. paludicolella Zeller, 1850, and A. orientella Staudinger, 1870 (sp. rev.), which are distributed in the western and eastern Mediterranean, respectively.},
}

Animals
DNA Barcoding, Taxonomic
Male
Female
Animal Distribution
Organ Size
*Moths/anatomy & histology/classification/genetics/growth & development
Animal Structures/anatomy & histology/growth & development
Body Size
Phylogeny
Greece
Italy

@article {pmid41119367,
year = {2025},
author = {Garzn-Ordua, IJ and Matson, TA and Vzquez, AM},
title = {Four new species of Acronyctodes Edwards (Geometridae: Ennominae) from Mesoamerica.},
journal = {Zootaxa},
volume = {5621},
number = {3},
pages = {335-352},
doi = {10.11646/zootaxa.5621.3.3},
pmid = {41119367},
issn = {1175-5334},
mesh = {Animals ; Male ; Female ; Animal Distribution ; Body Size ; *Moths/anatomy & histology/classification/growth & development/genetics ; Organ Size ; Animal Structures/anatomy & histology/growth & development ; Larva/anatomy & histology/classification/growth & development ; },
abstract = {Four new species of Acronyctodes are described and illustrated: A. gabrieli Matson sp. nov., A. asignum Matson sp. nov., A. bisbili Murillo-Vzquez sp. nov., and A. corrugata Matson & Garzn-Ordua sp. nov. Each species is distinguished by unique genitalic features, different DNA barcode data, and in some cases, interspecific larval color patterns. With the addition of these new species and the exclusion of Acronyctodes thinballa from the genus, the total number of species in Acronyctodes has doubled to eight.},
}

Animals
Male
Female
Animal Distribution
Body Size
*Moths/anatomy & histology/classification/growth & development/genetics
Organ Size
Animal Structures/anatomy & histology/growth & development
Larva/anatomy & histology/classification/growth & development

@article {pmid41119359,
year = {2025},
author = {Lu, M and Yao, Z and Liu, T},
title = {Leaf-mining moths of the genus Phyllonorycter Hbner (Lepidoptera: Gracillariidae: Lithocolletinae) associated with Juglandaceae in China, with descriptions of two new species and one newly recorded species.},
journal = {Zootaxa},
volume = {5621},
number = {4},
pages = {453-464},
doi = {10.11646/zootaxa.5621.4.4},
pmid = {41119359},
issn = {1175-5334},
mesh = {Animals ; *Moths/anatomy & histology/classification/growth & development/genetics ; China ; Female ; Male ; Body Size ; Organ Size ; Animal Distribution ; Animal Structures/anatomy & histology/growth & development ; Plant Leaves/parasitology ; Pupa/anatomy & histology/classification/growth & development ; },
abstract = {Until recently, no species of Phyllonorycter Hbner, 1822 had been recorded to feed on Juglandaceae in China. In this study, we describe two new species, P. stenopterae Lu & Liu, sp. nov. and P. tumoris Lu & Liu, sp. nov., both discovered in Shandong Province, China, feeding on Pterocarya stenoptera C. DC. (Juglandaceae). Additionally, this paper provides the first record of P. pterocaryae (Kumata, 1963) in China, along with its newly identified host plant, Pterocarya stenoptera. Detailed images of the adults, genitalia, pupae, host plants, leaf mines, and DNA barcode data of the new species are presented.},
}

Animals
*Moths/anatomy & histology/classification/growth & development/genetics
China
Female
Male
Body Size
Organ Size
Animal Distribution
Animal Structures/anatomy & histology/growth & development
Plant Leaves/parasitology
Pupa/anatomy & histology/classification/growth & development

@article {pmid41119347,
year = {2025},
author = {Zhang, ZC and Chen, JW and Zhang, Y and Lin, XL},
title = {First record of the genus Krenopelopia (Diptera: Chironomidae) from China, with a description of Krenopelopia wangi sp. nov.},
journal = {Zootaxa},
volume = {5673},
number = {2},
pages = {280-290},
doi = {10.11646/zootaxa.5673.2.7},
pmid = {41119347},
issn = {1175-5334},
mesh = {Animals ; Male ; China ; *Chironomidae/anatomy & histology/classification/genetics/growth & development ; Animal Distribution ; Female ; Body Size ; Organ Size ; Animal Structures/anatomy & histology/growth & development ; DNA Barcoding, Taxonomic ; },
abstract = {The genus Krenopelopia Fittkau, 1962 is recorded from China for the first time. Krenopelopia wangi Zhang & Lin, sp. nov. is described and illustrated as adults. Species status is supported by COI DNA barcodes. A key to adult males of Krenopelopia worldwide is presented.},
}

Animals
Male
China
*Chironomidae/anatomy & histology/classification/genetics/growth & development
Animal Distribution
Female
Body Size
Organ Size
Animal Structures/anatomy & histology/growth & development
DNA Barcoding, Taxonomic

@article {pmid41119323,
year = {2025},
author = {Pei, V and Bankowska, A and Goldschmidt, T and Hrsaker, K and Jovanovi, M and Kaitetzidou, E and Krakowiak, M and Kozowska, A and Michaloudi, E and Michoski, G and Milia, M and Pozojevi, I and Rewicz, T and Rusiniak, O and Sobolak, K and Szuko, I and Stryjecki, R and Stur, E and Szlauer-Ukaszewska, A and Szenejko, M and Zawal, A},
title = {Checklist of water mites from the Balkan Peninsula: second supplement, new DNA barcodes and description of a new species.},
journal = {Zootaxa},
volume = {5676},
number = {1},
pages = {1-74},
doi = {10.11646/zootaxa.5676.1.1},
pmid = {41119323},
issn = {1175-5334},
mesh = {Animals ; DNA Barcoding, Taxonomic ; *Mites/classification/anatomy & histology/genetics/growth & development ; Balkan Peninsula ; Male ; Female ; Animal Distribution ; Checklist ; Organ Size ; Body Size ; Animal Structures/anatomy & histology/growth & development ; },
abstract = {Knowledge of the water mite fauna in the Balkans is still very heterogeneous and far from complete. A compilation of published data is considered the first step in creating an up-to-date comprehensive checklist of water mites of the Balkans. In this supplement, we list all species reported from the Balkans from November 2017 to date and provide an updated checklist of water mites that inhabit the Balkan countries. With the additions in this study, the number of water mite species inhabiting the Balkan Peninsula has increased to 426 species and subspecies. One species, Sperchon balcanicus Pei, sp. nov. (Sperchontidae), is described as new to science. This study adds 299 new barcodes to the existing DNA barcode reference library that now includes 1032 COI DNA barcodes from the Balkans publicly available through the Barcode of Life Data Systems (BOLD). Still, only one-third of the currently known species of water mites in the Balkans now have reference barcodes in BOLD, indicating the need for future integrative research on the morphological and genetic diversity of Balkan water mites.},
}

Animals
DNA Barcoding, Taxonomic
*Mites/classification/anatomy & histology/genetics/growth & development
Balkan Peninsula
Male
Female
Animal Distribution
Checklist
Organ Size
Body Size
Animal Structures/anatomy & histology/growth & development

@article {pmid41119322,
year = {2025},
author = {Stadie, D and Lszl, GM and Fiebig, R},
title = {Integrative taxonomic revision of the Xenimpia-Psilocladia-Coenina generic complex with a review of the genus Procypha Warren, 1897 stat. rev. (Lepidoptera, Geometridae, Ennominae, Gonodontini).},
journal = {Zootaxa},
volume = {5677},
number = {1},
pages = {1-69},
doi = {10.11646/zootaxa.5677.1.1},
pmid = {41119322},
issn = {1175-5334},
mesh = {Animals ; Male ; *Moths/classification/anatomy & histology/growth & development/genetics ; Female ; Animal Distribution ; Organ Size ; Animal Structures/anatomy & histology/growth & development ; Body Size ; Phylogeny ; DNA Barcoding, Taxonomic ; Ecosystem ; },
abstract = {Using an integrative approach, the classification of the Afrotropical ennomine genera Xenimpia Warren, 1895, Coenina Walker, 1860, and Psilocladia Warren, 1898, is revised. As a result of the DNA barcoding combined with morphological data, eight distinct genera are delimited and characterised to include the current species content of Xenimpia, Coenina and Psilocladia: Bambenga Lszl, Stadie & Fiebig gen. nov. (type species Xenimpia repudiosa Prout, 1915), Arebatia Lszl, Stadie & Fiebig gen. nov. (type species Xenimpia tetracantha Herbulot, 1973), Claudimpia Lszl, Stadie & Fiebig gen. nov. (type species Xenimpia soricina Herbulot, 1973) and Paracoenina Lszl, Stadie & Fiebig gen. nov. (type species Coenina dentataria Swinhoe, 1904) are established. Furthermore, Xenimpia Warren, 1895 (type species: X. erosa Warren, 1895), Coenina Walker, 1860 (type species: Geometra poecilaria Herrich-Schffer, 1854) and Psilocladia Warren, 1898 (type species: P. obliquata Warren, 1898) are retained as valid genera and the latter is excluded from the tribe Gonodontini; in addition, Procypha Warren, 1897 stat. rev. (type species P. maculosata Warren, 1897) is reinstated from synonymy with Xenimpia. As a consequence of the revised generic classification, the following new combinations are established: Bambenga repudiosa (Prout, 1915) comb. nov., Arebatia albicaput (Fletcher, 1956) comb. nov., A. angusta (Prout, 1915) comb. nov., A. burgessi (Carcasson, 1964) comb. nov., A. ceres (Karisch, 2020) comb. nov., A. chalepa (Prout, 1915) comb. nov., A. clenchi (Viette, 1980) comb. nov., A. conformis (Warren, 1898) comb. nov., A. crassimedia (Herbulot, 1996) comb. nov., A. crassipecten (Herbulot, 1961) comb. nov., A. fletcheri (Herbulot, 1954) comb. nov., A. flexuosa (Herbulot, 1996) comb. nov., A. informis (Swinhoe, 1904) comb. nov., A. kala (Herbulot, 1973) comb. nov., A. karischi (Herbulot, 1996) comb. nov., A. loile (Carcasson, 1964) comb. nov., A. loxostigma (Prout, 1915) comb. nov., A. luxuriosa (Herbulot, 1961) comb. nov., A. spinosivalvis (Herbulot, 1996) comb. nov., A. tetracantha (Herbulot, 1973) comb. nov., A. vastata (Herbulot, 1996) comb. nov., Claudimpia diaereta (Prout, 1923) comb. nov., C. misogyna (Carcasson, 1962) comb. nov., C. soricina (Herbulot, 1973) comb. nov., Paracoenina dentataria (Swinhoe, 1904) comb. nov., P. aegyptiaca (Rebel, 1906) comb. nov., P. islamitica (Amsel, 1935) comb. nov., Procypha transmarina (Herbulot, 1961) comb. nov. and P. dohertyi (Herbulot, 1961) comb. nov. Moreover, the genus Procypha is taxonomically revised, including descriptions of five new taxa (P. paradohertyi sp. nov., P. strutzbergi sp. nov., P. strutzbergi caputgalli ssp. nov., P. insolita sp. nov. and P. meyi sp. nov.) and synonymisation of Xenimpia lactesignata Warren, 1914 syn. nov. regarded as a junior synonym of X. maculosata Warren, 1897. Based on diagnostic morphology, the genus Procypha is subdivided into four species groups. The early stages of Procypha maculosata, P. dohertyi and P. strutzbergi Stadie, Lszl & Fiebig sp. nov. are described and depicted, as well as wing patterns and genitalia of all species in this genus are figured. The paper is illustrated with two dendrograms inferred from ML genetic analyses, four distribution maps and 83 colour figures demonstrating the type species of each genus discussed and the adults, genitalia, early stages and habitats of Procypha taxa.},
}

Animals
Male
*Moths/classification/anatomy & histology/growth & development/genetics
Female
Animal Distribution
Organ Size
Animal Structures/anatomy & histology/growth & development
Body Size
Phylogeny
DNA Barcoding, Taxonomic
Ecosystem

@article {pmid41119317,
year = {2025},
author = {Bartolo, AG and Cassar, LF and Massa, B and Schembri, S and Ghana, S},
title = {Genetic analysis of a Mediterranean Red-Listed species Brachytrupes megacephalus (Lefbvre, 1827) (Orthoptera, Gryllidae).},
journal = {Zootaxa},
volume = {5679},
number = {1},
pages = {83-100},
doi = {10.11646/zootaxa.5679.1.4},
pmid = {41119317},
issn = {1175-5334},
mesh = {Animals ; *Gryllidae/genetics/classification/growth & development/anatomy & histology ; Phylogeny ; Male ; Animal Distribution ; Female ; Mediterranean Region ; Animal Structures/growth & development/anatomy & histology ; Body Size ; Organ Size ; Ecosystem ; },
abstract = {Brachytrupes megacephalus, the Giant Sand-dune Cricket, which has a restricted distribution across some locations in the Mediterranean and the more arid Sahara, typically colonises psammophilous biocoenoses within coastal and desert regions, including cultivated land where the substrate is characteristically sandy. Recent assessments reported that the species European population trend is in decline. Despite being a Red-listed species of conservation interest, it has not yet been studied genetically. The present study examines B. megacephalus specimens from Mediterranean locations in Sicily, Linosa, Sardinia, Malta, Gozo, and Libya, in addition to specimens of allied taxa from West Africa and the Far East. Barcode sequencing was carried out for fragments of the small (12SrRNA, ~400 bp) and large (16srRNA, ~500 bp) mitochondrial ribosomal subunits, the small nuclear ribosomal subunit (18SrRNA, ~650 bp), the large nuclear ribosomal unit (28SA, ~400 bp rRNA), the gene coding for H3 protein (H3, ~330 bp) and cytochrome c oxidase subunit 1 (CO1 ~600 bp). Subsequent phylogenetic analyses supported this clade as separate (at species level) from Brachytrupes membranaceus, which is the only other congener for which sequence data exists in online DNA libraries.},
}

Animals
*Gryllidae/genetics/classification/growth & development/anatomy & histology
Phylogeny
Male
Animal Distribution
Female
Mediterranean Region
Animal Structures/growth & development/anatomy & histology
Body Size
Organ Size
Ecosystem

@article {pmid41119313,
year = {2025},
author = {Huemer, P and Umpich, J},
title = {New species and interesting records of Spiniphallellus Bidzilya & Karsholt, 2008, from Kyrgyzstan (Lepidoptera, Gelechiidae).},
journal = {Zootaxa},
volume = {5679},
number = {1},
pages = {133-142},
doi = {10.11646/zootaxa.5679.1.8},
pmid = {41119313},
issn = {1175-5334},
mesh = {Animals ; Male ; Female ; Kyrgyzstan ; *Moths/anatomy & histology/classification/growth & development/genetics ; Animal Distribution ; Body Size ; Organ Size ; Animal Structures/anatomy & histology/growth & development ; DNA Barcoding, Taxonomic ; },
abstract = {Spiniphallellus minimus sp. nov., a new species of the recently described gelechiid genus Spiniphallellus Bidzilya & Karsholt, 2008, is described from Kyrgyzstan. Key diagnostic characters are found in the external morphology, particularly the short forewing length, and in the male genitalia, supported by divergence in DNA barcodes. In addition, S. stonisi Bidzilya & Karsholt, 2008, previously known only from the holotype, is reported from Kyrgyzstan for the first time and redescribed, including its previously unknown female. Adults and male genitalia of both species, as well as the female genitalia of S. stonisi, are illustrated.},
}

Animals
Male
Female
Kyrgyzstan
*Moths/anatomy & histology/classification/growth & development/genetics
Animal Distribution
Body Size
Organ Size
Animal Structures/anatomy & histology/growth & development
DNA Barcoding, Taxonomic

@article {pmid41119291,
year = {2025},
author = {Matsushima, Y and Blcu, MJ and Kakui, K},
title = {Atlantapseudes tridens sp. nov. (Tanaidacea: Apseudidae) from the Okinawa Trough, Japan, with a note on the taxonomic status of Atlantapseudes curvatus.},
journal = {Zootaxa},
volume = {5679},
number = {4},
pages = {484-500},
doi = {10.11646/zootaxa.5679.4.2},
pmid = {41119291},
issn = {1175-5334},
mesh = {Animals ; Japan ; Female ; Male ; Animal Distribution ; Body Size ; Animal Structures/anatomy & histology/growth & development ; Organ Size ; *Crustacea/classification/anatomy & histology/growth & development/genetics ; Ecosystem ; Phylogeny ; },
abstract = {The deep-sea apseudid genus Atlantapseudes Bcescu, 1978 currently contains six described species. The distinction between the type species (A. nigrichela Bcescu, 1978) and A. curvatus Esquete & Cunha, 2017 is problematic, because it is unclear whether the holotype for A. nigrichela has a tridentate or monodentate rostrum. We observed and redescribed the type material of A. nigrichela and concluded that A. nigrichela and A. curvatus are conspecific, i.e., the latter is a junior synonym of the former. Additionally, we amended the diagnosis of A. nigrichela and treated the form of A. nigrichela with a monodentate rostrum in Bcescu (1978) as an unidentified species. We also found that Atlantapseudes sp. sensu Kakui et al. (2011), the first record of this genus from the North Pacific, was undescribed; here we describe it as Atlantapseudes tridens sp. nov. The new species resembles A. nigrichela in having a tridentate rostrum but differs in having (1) pereonites 13 without posterolateral spines; (2) a tiny anterolateral spine on pereonite-6; (3) two distal bifurcate spiniform setae on the labial palp; and (4) one outer distal seta on maxillipedal palp article-2. We present a partial nucleotide sequence for the cytochrome c oxidase subunit I (COI) gene from A. tridens sp. nov. for future DNA barcoding.},
}

Animals
Japan
Female
Male
Animal Distribution
Body Size
Animal Structures/anatomy & histology/growth & development
Organ Size
*Crustacea/classification/anatomy & histology/growth & development/genetics
Ecosystem
Phylogeny

@article {pmid41119272,
year = {2025},
author = {Pei, V and Zawal, A and Bankowska, A and Arajo, R and Sugocki, U and Rewicz, T and Krakowiak, M and Michoski, G and Giro, D and Silva, LPD and Rfo, I and Raposeiro, PM and Ballini, L and Stryjecki, R and Ekrem, T and Ferreira, S},
title = {Exploring the water mite fauna (Acari, Hydrachnidia) of the Madeira archipelago: DNA Barcoding reveals a remarkable species endemicity.},
journal = {Zootaxa},
volume = {5621},
number = {5},
pages = {501-513},
doi = {10.11646/zootaxa.5621.5.1},
pmid = {41119272},
issn = {1175-5334},
mesh = {Animals ; DNA Barcoding, Taxonomic ; *Mites/classification/genetics/anatomy & histology/growth & development ; Animal Distribution ; Female ; Male ; Phylogeny ; Ecosystem ; },
abstract = {Water mites represent the group with the highest degree of endemism among all macroinvertebrates living in freshwater habitats of Madeira. The water mite fauna of this archipelago has been well known for a long time, but until now they have not been molecularly investigated. So far, 25 species of water mites have been recorded, most of them endemic to Madeira. The library presented here covers 584 COI DNA barcodes grouped into 23 Barcode Index Numbers (BINs), which represent the genetic barcodes of 23 species (more than 80% of the known Madeira water mite fauna). Our study shows that COI barcode clusters generated by the Barcode of Life Data Systems (BOLD) matches to morphological identifications of specimens, with one exception in the family Lebertiidae. A large-scale comparison of the new sequences with those available in public databases such as BOLD confirmed the uniqueness of the genetic diversity of water mites inhabiting Madeira. Neumania atlantida Lundblad, 1941, a species previously synonymized with N. uncinata Walter, 1927, is resurrected as a valid species. Additionally, genetic data revealed that Sperchon brevirostris Koenike, 1895, a species common in freshwaters of Europe and Macaronesia, consists of multiple genetic lineages, one of which is restricted to Madeira. Finally, our research revealed three species new to the water mite fauna of Madeira, i.e., Hydrachna skorikowi Piersig, 1900, Arrenurus bicuspidator Berlese, 1885 and Lebertia algeriensis Lundblad, 1942. The latter species, found to be common in the running waters of the island, may be the first species of water mite documented as potentially, if not invasive, then non-indigenous in freshwater ecosystems of Madeira.},
}

Animals
DNA Barcoding, Taxonomic
*Mites/classification/genetics/anatomy & histology/growth & development
Animal Distribution
Female
Male
Phylogeny
Ecosystem

@article {pmid41119270,
year = {2025},
author = {Ancillotto, L and Serafini, E and Viviano, A and Labadessa, R and Martino, J and Annessi, M and Bruni, G and Repetto, E and Nabholz, B and Mori, E and Baratti, M},
title = {DNA-barcoding and ecological niche analysis of Wart Biters (Decticus spp.) from an endemism hotspot (Tettigoniidae: Tettigoniinae).},
journal = {Zootaxa},
volume = {5621},
number = {5},
pages = {547-559},
doi = {10.11646/zootaxa.5621.5.3},
pmid = {41119270},
issn = {1175-5334},
mesh = {Animals ; Ecosystem ; Phylogeny ; Male ; Animal Distribution ; Female ; DNA Barcoding, Taxonomic ; *Orthoptera/genetics/classification/growth & development/physiology ; Italy ; DNA, Mitochondrial/genetics ; },
abstract = {Understanding biogeographical patterns is a challenging task, particularly in the case of poorly studied organisms, whose phylogenetic affinities and ecological needs are not yet understood. Such a case is common among invertebrates and particularly among insects, whose extensive diversity may hamper full and deep comprehension of their ecological and evolutionary patterns. Within insects, orthopterans also represent a relatively poorly studied group. Building knowledge upon biogeography and evolution of orthopterans may provide key insights to their conservation, especially in the case of range-restricted taxa that are inherently more vulnerable, and prone to extinction. In this study we thus applied an integrated approach, combining DNA-barcoding and ecological niche modelling, for investigating the diversity of wart biters (Orthoptera Tettigoniidae, genus Decticus). We particularly aimed at providing a first screening of the molecular identity of species within the genus, and an assessment of their macro-ecological needs. We analysed mitochondrial DNA (cytochrome oxidase I COI) and bioclimatic niche data for the four Decticus species that occur in the Italian Peninsula (D. verrucivorus, D. albifrons, D. loudoni, and D. aprutianus), reconstructing their phylogenetic relationships based on molecular approaches, and comparing their ecological preferences. We provide COI sequences for all the species found in Italy, including the first one available for two endemic ones. We found a clear molecular differentiation among the four species, with D. albifrons being the most distinct and basal taxon, as based on the COI sequence adopted. Our results also bring evidence of significant ecological niche differentiation, with each species occupying a distinct portion of the climatic space available within Italy. Notably, the two range-restricted and short-winged species, D. loudoni and D. aprutianus, result as sister taxa according to COI, and exhibit greater ecological specialisation compared to the more widespread species. Besides, we also highlight significant intra-taxon diversity for both D. verrucivorus sensu stricto and D. albifrons, suggesting that further research on potential intraspecific variability may be needed to clarify the taxonomic position of Italian populations. The observed genetic divergence and ecological niche differentiation found among wart biters suggest that adaptation to different ecological conditions may have played a role in the diversification of these range-restricted species. Our results confirmed the importance of the Italian Peninsula as a biodiversity hotspot for Orthoptera and provide a solid baseline for more in-depth molecular studies, besides providing novel sequences that may be used for e.g., DNA metabarcoding or eDNA campaigns. Further research is in fact needed to explore the specific ecological factors driving niche differentiation in this and other taxa, and to investigate the evolutionary pathways that led to the genus current diversity and distribution.},
}

Animals
Ecosystem
Phylogeny
Male
Animal Distribution
Female
DNA Barcoding, Taxonomic
*Orthoptera/genetics/classification/growth & development/physiology
Italy
DNA, Mitochondrial/genetics

@article {pmid41119269,
year = {2025},
author = {Xu, ZB and Grehan, JR and Yang, ZY and Gan, HL and Hu, SJ},
title = {Two new species of Palpifer Hampson (Lepidoptera: Hepialidae) from Yunnan, China.},
journal = {Zootaxa},
volume = {5621},
number = {5},
pages = {560-570},
doi = {10.11646/zootaxa.5621.5.4},
pmid = {41119269},
issn = {1175-5334},
mesh = {Animals ; Male ; China ; Female ; *Moths/anatomy & histology/classification/growth & development/genetics ; Animal Distribution ; Animal Structures/anatomy & histology/growth & development ; Organ Size ; Body Size ; },
abstract = {Two new species of the genus Palpifer Hampson, 1893 are described. The first species, named P. qinglai sp. nov. from Yunnan University in Kunming, Yunnan, China, is similar to P. sexnotatus (Moore, 1879) but distinguished by differences in forewing pattern, male genitalia structure and DNA barcode sequence. The second species, named P. bazi sp. nov. from Mengzi, Honghe, Yunnan, China, resembles P. boonei Grehan & Mielke, 2019 but also differs in the same characteristics.},
}

Animals
Male
China
Female
*Moths/anatomy & histology/classification/growth & development/genetics
Animal Distribution
Animal Structures/anatomy & histology/growth & development
Organ Size
Body Size

@article {pmid41119263,
year = {2025},
author = {Shafaie, S and Pekr, S and Ortiz, D},
title = {Integrative taxonomy of the Iberian Zodarion species of the rubidum and styliferum groups (Araneae: Zodariidae).},
journal = {Zootaxa},
volume = {5624},
number = {1},
pages = {1-69},
doi = {10.11646/zootaxa.5624.1.1},
pmid = {41119263},
issn = {1175-5334},
mesh = {Animals ; *Spiders/classification/anatomy & histology/genetics/growth & development ; Male ; Female ; Phylogeny ; Animal Distribution ; Animal Structures/growth & development/anatomy & histology ; Body Size ; Organ Size ; Spain ; },
abstract = {Zodarion, one of the most diverse zodariid spider genera, has recently garnered significant interest due to its peculiar adaptations for stringent ant-eating specialization. However, species identification within this genus remains challenging, as many Zodarion species are still insufficiently documented. This study revisits the taxonomy of Iberian species within the rubidum and styliferum groups, which together comprise 25 of the 171 species in the genus. Detailed morphological documentation is provided, including photographs and SEM images of key taxonomic characters, presented here for the first time. Molecular characterizations were also conducted to facilitate future species identification and to investigate patterns of mitochondrial variation within and between species. Notably, Z. extraneum has been reinstated as a distinct species. The male of Z. extraneum and the female of Z. alentejanum are described for the first time, while the female of Z. viduum is redescribed. Additionally, the species Z. alentejanum, Z. bosmansi, Z. guadianense and Z. lusitanicum have been assigned to the styliferum group. Three new synonyms are proposed: Z. brevicephalus with Z. styliferum, Z. duriense with Z. bacelarae, and Z. parastyliferum with Z. extraneum. Pairwise interspecific divergences between individual sequences generally exceeded intraspecific variation, and the mitochondrial gene tree recovered all but one species as monophyletic groups, underscoring the utility of molecular barcoding for identifying Zodarion species. However, interspecific distances between consensus sequences often blurred species boundaries. Our integrative taxonomic study provides tools for improved identification of Zodarion species, facilitating further research into their biology.},
}

Animals
*Spiders/classification/anatomy & histology/genetics/growth & development
Male
Female
Phylogeny
Animal Distribution
Animal Structures/growth & development/anatomy & histology
Body Size
Organ Size
Spain