@article {pmid35596005, year = {2022}, author = {Shchenkov, SV and Sokolov, SG and Tsushko, KM and Denisova, SA}, title = {Is Gymnophallus Odhner, 1900 (Trematoda: Gymnophallidae) polyphyletic? A new hypothesis based on phylogenetic position of Gymnophallus deliciosus (Olsson, 1893).}, journal = {Parasitology research}, volume = {}, number = {}, pages = {}, pmid = {35596005}, issn = {1432-1955}, abstract = {Gymnophallus deliciosus is a type species of the genus Gymnophallus. We collected this trematode species from gallbladder of Larus argentatus caught in the White Sea (Kandalaksha Bay, Chupa Inlet) and obtained the sequences of its nuclear 18S and 28S rDNA genes. Our recently obtained phylogenetic data support a sister group relationship of this species with G. choledochus. However, other species of the genus Gymnophallus-G. australis (KM246854) and G. minutus (KM268111)-do not branch with the group G. choledochus + G. deliciosus or with each other. Our study revealed that the genus Gynmnophallus is probably a polyphyletic taxon, and the genus affiliation of its representatives should be re-examined in future.}, }
@article {pmid35581187, year = {2022}, author = {Demeter, F and Zanolli, C and Westaway, KE and Joannes-Boyau, R and Duringer, P and Morley, MW and Welker, F and Rüther, PL and Skinner, MM and McColl, H and Gaunitz, C and Vinner, L and Dunn, TE and Olsen, JV and Sikora, M and Ponche, JL and Suzzoni, E and Frangeul, S and Boesch, Q and Antoine, PO and Pan, L and Xing, S and Zhao, JX and Bailey, RM and Boualaphane, S and Sichanthongtip, P and Sihanam, D and Patole-Edoumba, E and Aubaile, F and Crozier, F and Bourgon, N and Zachwieja, A and Luangkhoth, T and Souksavatdy, V and Sayavongkhamdy, T and Cappellini, E and Bacon, AM and Hublin, JJ and Willerslev, E and Shackelford, L}, title = {A Middle Pleistocene Denisovan molar from the Annamite Chain of northern Laos.}, journal = {Nature communications}, volume = {13}, number = {1}, pages = {2557}, pmid = {35581187}, issn = {2041-1723}, abstract = {The Pleistocene presence of the genus Homo in continental Southeast Asia is primarily evidenced by a sparse stone tool record and rare human remains. Here we report a Middle Pleistocene hominin specimen from Laos, with the discovery of a molar from the Tam Ngu Hao 2 (Cobra Cave) limestone cave in the Annamite Mountains. The age of the fossil-bearing breccia ranges between 164-131 kyr, based on the Bayesian modelling of luminescence dating of the sedimentary matrix from which it was recovered, U-series dating of an overlying flowstone, and U-series-ESR dating of associated faunal teeth. Analyses of the internal structure of the molar in tandem with palaeoproteomic analyses of the enamel indicate that the tooth derives from a young, likely female, Homo individual. The close morphological affinities with the Xiahe specimen from China indicate that they belong to the same taxon and that Tam Ngu Hao 2 most likely represents a Denisovan.}, }
@article {pmid35406627, year = {2022}, author = {Skryabin, GO and Komelkov, AV and Zhordania, KI and Bagrov, DV and Vinokurova, SV and Galetsky, SA and Elkina, NV and Denisova, DA and Enikeev, AD and Tchevkina, EM}, title = {Extracellular Vesicles from Uterine Aspirates Represent a Promising Source for Screening Markers of Gynecologic Cancers.}, journal = {Cells}, volume = {11}, number = {7}, pages = {}, doi = {10.3390/cells11071064}, pmid = {35406627}, issn = {2073-4409}, abstract = {Extracellular vesicles (EVs), including exosomes, are key factors of intercellular communication, performing both local and distant transfers of bioactive molecules. The increasingly obvious role of EVs in carcinogenesis, similarity of molecular signatures with parental cells, precise selection and high stability of cargo molecules make exosomes a promising source of liquid biopsy markers for cancer diagnosis. The uterine cavity fluid, unlike blood, urine and other body fluids commonly used to study EVs, is of local origin and therefore enriched in EVs secreted by cells of the female reproductive tract. Here, we show that EVs, including those corresponding to exosomes, could be isolated from individual samples of uterine aspirates (UA) obtained from epithelial ovarian cancer (EOC) patients and healthy donors using the ultracentrifugation technique. First, the conducted profiling of small RNAs (small RNA-seq) from UA-derived EVs demonstrated the presence of non-coding RNA molecules belonging to various classes. The analysis of the miRNA content in EVs from UA performed on a pilot sample revealed significant differences in the expression levels of a number of miRNAs in EVs obtained from EOC patients compared to healthy individuals. The results open up prospects for using UA-derived EVs as a source of markers for the diagnostics of gynecological cancers, including EOC.}, }
@article {pmid35359699, year = {2022}, author = {Molodtseva, AS and Makunin, AI and Salomashkina, VV and Kichigin, IG and Vorobieva, NV and Vasiliev, SK and Shunkov, MV and Tishkin, AA and Grushin, SP and Anijalg, P and Tammeleht, E and Keis, M and Boeskorov, GG and Mamaev, N and Okhlopkov, IM and Kryukov, AP and Lyapunova, EA and Kholodova, MV and Seryodkin, IV and Saarma, U and Trifonov, VA and Graphodatsky, AS}, title = {Phylogeography of ancient and modern brown bears from eastern Eurasia.}, journal = {Biological journal of the Linnean Society. Linnean Society of London}, volume = {135}, number = {4}, pages = {722-733}, doi = {10.1093/biolinnean/blac009}, pmid = {35359699}, issn = {0024-4066}, abstract = {The brown bear (Ursus arctos) is an iconic carnivoran species of the Northern Hemisphere. Its population history has been studied extensively using mitochondrial markers, which demonstrated signatures of multiple waves of migration, arguably connected with glaciation periods. Among Eurasian brown bears, Siberian populations remain understudied. We have sequenced complete mitochondrial genomes of four ancient (~4.5-40 kya) bears from South Siberia and 19 modern bears from South Siberia and the Russian Far East. Reconstruction of phylogenetic relationships between haplotypes and evaluation of modern population structure have demonstrated that all the studied samples belong to the most widespread Eurasian clade 3. One of the ancient haplotypes takes a basal position relative to the whole of clade 3; the second is basal to the haplogroup 3a (the most common subclade), and two others belong to clades 3a1 and 3b. Modern Siberian bears retain at least some of this diversity; apart from the most common haplogroup 3a, we demonstrate the presence of clade 3b, which was previously found mainly in mainland Eurasia and Northern Japan. Our findings highlight the importance of South Siberia as a refugium for northern Eurasian brown bears and further corroborate the hypothesis of several waves of migration in the Pleistocene.}, }
@article {pmid35327421, year = {2022}, author = {Sobolev, VV and Soboleva, AG and Denisova, EV and Pechatnikova, EA and Dvoryankova, E and Korsunskaya, IM and Mezentsev, A}, title = {Proteomic Studies of Psoriasis.}, journal = {Biomedicines}, volume = {10}, number = {3}, pages = {}, doi = {10.3390/biomedicines10030619}, pmid = {35327421}, issn = {2227-9059}, abstract = {In this review paper, we discuss the contribution of proteomic studies to the discovery of disease-specific biomarkers to monitor the disease and evaluate available treatment options for psoriasis. Psoriasis is one of the most prevalent skin disorders driven by a Th17-specific immune response. Although potential patients have a genetic predisposition to psoriasis, the etiology of the disease remains unknown. During the last two decades, proteomics became deeply integrated with psoriatic research. The data obtained in proteomic studies facilitated the discovery of novel mechanisms and the verification of many experimental hypotheses of the disease pathogenesis. The detailed data analysis revealed multiple differentially expressed proteins and significant changes in proteome associated with the disease and drug efficacy. In this respect, there is a need for proteomic studies to characterize the role of the disease-specific biomarkers in the pathogenesis of psoriasis, develop clinical applications to choose the most efficient treatment options and monitor the therapeutic response.}, }
@article {pmid35319532, year = {2022}, author = {Dengler, NF and Ferraresi, S and Rochkind, S and Denisova, N and Garozzo, D and Heinen, C and Alimehmeti, R and Capone, C and Barone, DG and Zdunczyk, A and Pedro, MT and Antoniadis, G and Kaiser, R and Dubuisson, A and Kretschmer, T and Rasulic, L}, title = {Thoracic Outlet Syndrome Part I: Systematic Review of the Literature and Consensus on Anatomy, Diagnosis, and Classification of Thoracic Outlet Syndrome by the European Association of Neurosurgical Societies' Section of Peripheral Nerve Surgery.}, journal = {Neurosurgery}, volume = {}, number = {}, pages = {}, pmid = {35319532}, issn = {1524-4040}, abstract = {BACKGROUND: Although numerous articles have been published not only on the classification of thoracic outlet syndrome (TOS) but also on diagnostic standards, timing, and type of surgical intervention, there still remains some controversy because of the lack of level 1 evidence. So far, attempts to generate uniform reporting standards have not yielded conclusive results.<br><br>OBJECTIVE: To systematically review the body of evidence and reach a consensus among neurosurgeons experienced in TOS regarding anatomy, diagnosis, and classification.<br><br>METHODS: A systematic literature search on PubMed/MEDLINE was performed on February 13, 2021, yielding 2853 results. Abstracts were screened and classified. Recommendations were developed in a meeting held online on February 10, 2021, and refined according to the Delphi consensus method.<br><br>RESULTS: Six randomized controlled trials (on surgical, conservative, and injection therapies), 4 "guideline" articles (on imaging and reporting standards), 5 observational studies (on diagnostics, hierarchic designs of physiotherapy vs surgery, and quality of life outcomes), and 6 meta-analyses were identified. The European Association of Neurosurgical Societies' section of peripheral nerve surgery established 18 statements regarding anatomy, diagnosis, and classification of TOS with agreement levels of 98.4 % (±3.0).<br><br>CONCLUSION: Because of the lack of level 1 evidence, consensus statements on anatomy, diagnosis, and classification of TOS from experts of the section of peripheral nerve surgery of the European Association of Neurosurgical Societies were developed with the Delphi method. Further work on reporting standards, prospective data collections, therapy, and long-term outcome is necessary.}, }
@article {pmid35236068, year = {2022}, author = {Zavgorudko, VN and Sidorenko, SV and Kortelev, VV and Zavgorudko, TI and Zavgorudko, GV and Senkevich, OA and Denisova, EV}, title = {[Tumnin mineral spring. History of development].}, journal = {Voprosy kurortologii, fizioterapii, i lechebnoi fizicheskoi kultury}, volume = {99}, number = {1}, pages = {64-68}, doi = {10.17116/kurort20229901164}, pmid = {35236068}, issn = {0042-8787}, mesh = {*Balneology ; Female ; Health Resorts ; Humans ; *Mineral Waters/therapeutic use ; Minerals ; *Skin Diseases/drug therapy ; }, abstract = {The article dwells upon the history of the discovery of the Tumnin mineral spring, the establishment and development of the Far Eastern health resort «Goryachy Klyuch,» located in the basin of Chope creek, a tributary of the largest river in the eastern macroslope of Sikhote Alin, Tumnin river, located 25 km from the Strait of Tartary. A historical sketch since the first mentioning of the Tumnin mineral spring from 1903 to the present day, as well as the results of hydrogeological expeditions to determine the chemical composition and α-activity of Tumnin mineral water at different periods, are presented. A contribution of a geological expedition that established a large deep-lying tectonic structure permeable to upwelling thermal water flows is described. The role of the staff of the physiotherapy and balneology department of the Khabarovsk Medical Institute in the study of the mechanism of action and clinical effectiveness of the Tumnin mineral water is addressed. A balneological characteristic of nitric and siliceous thermal water, the basic therapeutic factor of «Goryachy Klyuch» health resort, which has always been popular among the Far East residents, but gained special importance and appreciation of patients during the pandemic of new coronavirus infection, is given. Currently, in the health resort «Goryachy Klyuch», patients with skin diseases, musculoskeletal, gynecologic, neurologic diseases, digestive tract disorders, metabolic conditions, upper airways, cardiovascular disorders, occupational diseases are treated using balneotherapy and other methods of non-drug therapy. At present, the health resort «Goryachy Klyuch» is going through a difficult but interesting period of improvement of recreation opportunities for the Far East residents.}, }
@article {pmid34718708, year = {2022}, author = {Saitou, M and Masuda, N and Gokcumen, O}, title = {Similarity-Based Analysis of Allele Frequency Distribution among Multiple Populations Identifies Adaptive Genomic Structural Variants.}, journal = {Molecular biology and evolution}, volume = {39}, number = {3}, pages = {}, doi = {10.1093/molbev/msab313}, pmid = {34718708}, issn = {1537-1719}, abstract = {Structural variants have a considerable impact on human genomic diversity. However, their evolutionary history remains mostly unexplored. Here, we developed a new method to identify potentially adaptive structural variants based on a similarity-based analysis that incorporates genotype frequency data from 26 populations simultaneously. Using this method, we analyzed 57,629 structural variants and identified 576 structural variants that show unusual population differentiation. Of these putatively adaptive structural variants, we further showed that 24 variants are multiallelic and overlap with coding sequences, and 20 variants are significantly associated with GWAS traits. Closer inspection of the haplotypic variation associated with these putatively adaptive and functional structural variants reveals deviations from neutral expectations due to: 1) population differentiation of rapidly evolving multiallelic variants, 2) incomplete sweeps, and 3) recent population-specific negative selection. Overall, our study provides new methodological insights, documents hundreds of putatively adaptive variants, and introduces evolutionary models that may better explain the complex evolution of structural variants.}, }
@article {pmid35197262, year = {2022}, author = {Yogeswaran, K and Furtado, JM and Bodaghi, B and Matthews, JM and ,  and Smith, JR}, title = {Current practice in the management of ocular toxoplasmosis.}, journal = {The British journal of ophthalmology}, volume = {}, number = {}, pages = {}, doi = {10.1136/bjophthalmol-2022-321091}, pmid = {35197262}, issn = {1468-2079}, abstract = {BACKGROUND: Ocular toxoplasmosis is common across all regions of the world. Understanding of the epidemiology and approach to diagnosis and treatment have evolved recently. In November 2020, an international group of uveitis-specialised ophthalmologists formed the International Ocular Toxoplasmosis Study Group to define current practice.<br><br>METHODS: 192 Study Group members from 48 countries completed a 36-item survey on clinical features, use of investigations, indications for treatment, systemic and intravitreal treatment with antiparasitic drugs and corticosteroids, and approach to follow-up and preventive therapy.<br><br>RESULTS: For 77.1% of members, unilateral retinochoroiditis adjacent to a pigmented scar accounted for over 60% of presentations, but diverse atypical presentations were also reported. Common complications included persistent vitreous opacities, epiretinal membrane, cataract, and ocular hypertension or glaucoma. Most members used clinical examination with (56.8%) or without (35.9%) serology to diagnose typical disease but relied on intraocular fluid testing-usually PCR-in atypical cases (68.8%). 66.1% of members treated all non-pregnant patients, while 33.9% treated selected patients. Oral trimethoprim-sulfamethoxazole was first-line therapy for 66.7% of members, and 60.9% had experience using intravitreal clindamycin. Corticosteroid drugs were administered systemically by 97.4%; 24.7% also injected corticosteroid intravitreally, almost always in combination with an antimicrobial drug (72.3%). The majority of members followed up all (60.4%) or selected (35.9%) patients after resolution of acute disease, and prophylaxis against recurrence with trimethoprim-sulfamethoxazole was prescribed to selected patients by 69.8%.<br><br>CONCLUSION: Our report presents a current management approach for ocular toxoplasmosis, as practised by a large international group of uveitis-specialised ophthalmologists.}, }
@article {pmid35175470, year = {2022}, author = {Voron'ko, OE and Baskaev, KK and Sobolev, VV and Denisova, EV and Korsunskaya, IM}, title = {Genetic Markers of Therapeutic Efficacy of Methotrexate in Patients with Psoriasis.}, journal = {Bulletin of experimental biology and medicine}, volume = {}, number = {}, pages = {}, pmid = {35175470}, issn = {1573-8221}, abstract = {We studied the effect of C677T and A1298C polymorphisms of the MTHFR gene and 2R/3R polymorphisms of the TYMS gene on the sensitivity to methotrexate in patients with psoriasis (n=139). It was shown that genotype 3R/3R TYMS (OR 8.86, 95%CI 2.00-39.22) and complex genotypes MTHFR1298:A;TYMS:3R (OR 8.20, 95%CI 2.36-28.48) and MTHFR677:C;TYMS:3R (OR 5.40, 95%CI 1.95-14.94) were associated with sensitivity to methotrexate, while genotype 2R/2R TYMS (OR 8.20, 95%CI 2.36-28.48) and complex genotypes MTHFR1298:C;MTHFR677:T;TYMS:2R (OR 0.18, 95%CI 0.06-0.56) and MTHFR1298:C;MTHFR677:T (OR 0.23, 95%CI 0.09-0.59) were associated with resistance to methotrexate. The results can be used for preventive assessment of the effectiveness of methotrexate treatment in patients with psoriasis.}, }
@article {pmid35163644, year = {2022}, author = {Denisova, KR and Orlov, NA and Yakimov, SA and Kryukova, EA and Dolgikh, DA and Kirpichnikov, MP and Feofanov, AV and Nekrasova, OV}, title = {GFP-Margatoxin, a Genetically Encoded Fluorescent Ligand to Probe Affinity of Kv1.3 Channel Blockers.}, journal = {International journal of molecular sciences}, volume = {23}, number = {3}, pages = {}, doi = {10.3390/ijms23031724}, pmid = {35163644}, issn = {1422-0067}, support = {Research Project N 075-15-2020-795, local identifier 13.1902.21.0027//Ministry of Science and Higher Education of Russia/ ; }, abstract = {Peptide pore blockers and their fluorescent derivatives are useful molecular probes to study the structure and functions of the voltage-gated potassium Kv1.3 channel, which is considered as a pharmacological target in the treatment of autoimmune and neurological disorders. We present Kv1.3 fluorescent ligand, GFP-MgTx, constructed on the basis of green fluorescent protein (GFP) and margatoxin (MgTx), the peptide, which is widely used in physiological studies of Kv1.3. Expression of the fluorescent ligand in E. coli cells resulted in correctly folded and functionally active GFP-MgTx with a yield of 30 mg per 1 L of culture. Complex of GFP-MgTx with the Kv1.3 binding site is reported to have the dissociation constant of 11 ± 2 nM. GFP-MgTx as a component of an analytical system based on the hybrid KcsA-Kv1.3 channel is shown to be applicable to recognize Kv1.3 pore blockers of peptide origin and to evaluate their affinities to Kv1.3. GFP-MgTx can be used in screening and pre-selection of Kv1.3 channel blockers as potential drug candidates.}, }
@article {pmid35160434, year = {2022}, author = {Roenko, AV and Nikiforov, RY and Gringolts, ML and Belov, NA and Denisova, YI and Shandryuk, GA and Bondarenko, GN and Kudryavtsev, YV and Finkelshtein, ES}, title = {Olefin-Metathesis-Derived Norbornene-Ethylene-Vinyl Acetate/Vinyl Alcohol Multiblock Copolymers: Impact of the Copolymer Structure on the Gas Permeation Properties.}, journal = {Polymers}, volume = {14}, number = {3}, pages = {}, doi = {10.3390/polym14030444}, pmid = {35160434}, issn = {2073-4360}, support = {20-03-00703//Russian Foundation for Basic Research/ ; }, abstract = {Commercial metathesis polynorbornene is used for the fabrication of high-damping coatings and bulk materials that dissipate vibration and impact energies. Functionalization of this non-polar polymer can improve its adhesive, gas barrier, and other properties, thereby potentially expanding its application area. With this aim, the post-modification of polynorbornene was carried out by inserting ethylene-vinyl acetate-vinyl alcohol blocks into its backbone via the cross-metathesis of polynorbornene with poly(5-acetoxy-1-octenylene) and subsequent deacetylation and hydrogenation of the obtained multiblock copolymers. For the first time, epoxy groups were introduced into the main chains of these copolymers, followed by the oxirane ring opening reaction. The influence of post-modification on the thermal, gas separation, and mechanical properties of the new copolymers was studied. It was shown that the gas permeability of the copolymer significantly depends on its composition, as well as on the amounts of hydroxyl and epoxy groups. The developed methods efficiently improve the barrier properties, reducing the oxygen permeability by 15-33 times in comparison with polynorbornene. The obtained results are promising for various applications and can be extended to a broader family of polydienes and other polymers containing backbone double bonds.}, }
@article {pmid35143674, year = {2022}, author = {Göllner, T and Larena, M and Kutanan, W and Lukas, H and Fieder, M and Schaschl, H}, title = {Unveiling the Genetic History of the Maniq, a primary hunter-gatherer society.}, journal = {Genome biology and evolution}, volume = {}, number = {}, pages = {}, doi = {10.1093/gbe/evac021}, pmid = {35143674}, issn = {1759-6653}, abstract = {The Maniq of southern Thailand is one of the last remaining practicing hunter-gatherer communities in the world. However, our knowledge on their genetic origins and demographic history is still largely limited. We present here the genotype data covering ∼2.3 million SNPs of eleven unrelated Maniq individuals. Our analyses reveal the Maniq to be closely related to the Semang populations of Malaysia (Malay Negritos), who altogether carry an Andamanese-related ancestry linked to the ancient Hòabìnhian hunter-gatherers of Mainland Southeast Asia (MSEA). Moreover, the Maniq possess ∼35% East Asian-related ancestry, likely brought about by recent admixture with surrounding agriculturist communities in the region. In addition, the Maniq exhibit one of the highest levels of genetic differentiation found among living human populations, indicative of their small population size and historical practice of endogamy. Similar to other hunter-gatherer populations of MSEA, we also find the Maniq to possess low levels of Neanderthal ancestry and undetectable levels of Denisovan ancestry. Altogether, we reveal the Maniq to be a Semang group that experienced intense genetic drift and exhibits signs of ancient Hòabìnhian ancestry.}, }
@article {pmid35075148, year = {2022}, author = {Morley, MW and Goldberg, P and Uliyanov, VA and Kozlikin, MB and Shunkov, MV and Derevianko, AP and Jacobs, Z and Roberts, RG}, title = {Author Correction: Hominin and animal activities in the microstratigraphic record from Denisova Cave (Altai Mountains, Russia).}, journal = {Scientific reports}, volume = {12}, number = {1}, pages = {1545}, doi = {10.1038/s41598-021-03251-6}, pmid = {35075148}, issn = {2045-2322}, }
@article {pmid34971813, year = {2021}, author = {Shchenkov, SV and Sokolov, SG and Denisova, SA}, title = {Phylogenetic position of Atriophallophorus minutus (Trematoda: Microphallidae), the type-species of the genus Atriophallophorus Deblock &amp; Rosé, 1964, based on partial 28S rDNA gene sequence.}, journal = {Parasitology international}, volume = {87}, number = {}, pages = {102534}, doi = {10.1016/j.parint.2021.102534}, pmid = {34971813}, issn = {1873-0329}, abstract = {We found metacercariae of a microphallid trematode Atriophallophorus minutus in freshwater snails Bithynia tentaculata. In this study, we provide a morphological description of whole-mount specimens and semi-thin sections of experimentally grown adults of this species. Our morphological examination supports the idea that the adults of Atriophallophorus spp. have a ventral sucker with a sinistral interruption of the outer edge. In the 28S rDNA gene-based phylogenetic analyses, our specimens of A. minutus were grouped into Atriophallophorus spp. clade, as a sister taxon to Atriophallophorus winterbourni + Atriophallophorus sp. Analysis of the pairwise genetic distances between coI mtDNA gene sequences revealed a low divergence between the two specimens of A. minutus (1.1%) and a greater divergence (up to 16.6%) between them and A. winterbourni. Since other Atriophallophorus spp. are known to have a strict specificity to the polyvalent intermediate host, we suggest that A. minutus reported from different snail species may represent a complex of cryptic species.}, }
@article {pmid34969841, year = {2022}, author = {Massilani, D and Morley, MW and Mentzer, SM and Aldeias, V and Vernot, B and Miller, C and Stahlschmidt, M and Kozlikin, MB and Shunkov, MV and Derevianko, AP and Conard, NJ and Wurz, S and Henshilwood, CS and Vasquez, J and Essel, E and Nagel, S and Richter, J and Nickel, B and Roberts, RG and Pääbo, S and Slon, V and Goldberg, P and Meyer, M}, title = {Microstratigraphic preservation of ancient faunal and hominin DNA in Pleistocene cave sediments.}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {119}, number = {1}, pages = {}, doi = {10.1073/pnas.2113666118}, pmid = {34969841}, issn = {1091-6490}, abstract = {Ancient DNA recovered from Pleistocene sediments represents a rich resource for the study of past hominin and environmental diversity. However, little is known about how DNA is preserved in sediments and the extent to which it may be translocated between archaeological strata. Here, we investigate DNA preservation in 47 blocks of resin-impregnated archaeological sediment collected over the last four decades for micromorphological analyses at 13 prehistoric sites in Europe, Asia, Africa, and North America and show that such blocks can preserve DNA of hominins and other mammals. Extensive microsampling of sediment blocks from Denisova Cave in the Altai Mountains reveals that the taxonomic composition of mammalian DNA differs drastically at the millimeter-scale and that DNA is concentrated in small particles, especially in fragments of bone and feces (coprolites), suggesting that these are substantial sources of DNA in sediments. Three microsamples taken in close proximity in one of the blocks yielded Neanderthal DNA from at least two male individuals closely related to Denisova 5, a Neanderthal toe bone previously recovered from the same layer. Our work indicates that DNA can remain stably localized in sediments over time and provides a means of linking genetic information to the archaeological and ecological records on a microstratigraphic scale.}, }
@article {pmid34964332, year = {2021}, author = {Soldatskiy, YL and Bulynko, SA and Denisova, OA and Edgem, SR and Kovalets, ES}, title = {[Features of the clinic, diagnosis and treatment of parapharyngeal abscesses in children: analysis of 121 observations].}, journal = {Vestnik otorinolaringologii}, volume = {86}, number = {6}, pages = {62-68}, doi = {10.17116/otorino20218606162}, pmid = {34964332}, issn = {0042-4668}, abstract = {Parapharyngeal and retropharyngeal abscesses (PPA) in children are a rare pathology, for the diagnosis of which it is necessary to use additional instrumental examination methods. The tactics of treating patients remains a subject of discussion.<br><br>OBJECTIVE: To analyze the features of the clinic, diagnosis and treatment of PPA in children.<br><br>MATERIAL AND METHODS: According to the hospital database, a retrospective analysis of the medical histories of children discharged from the clinic with a diagnosis of "J39.0 Retropharyngeal and parapharyngeal abscess" was carried out in the period from 01.01.14 to 31.12.19. In all cases, the diagnosis was confirmed by computed tomography (CT) data with contrast enhancement. Complaints at the time of treatment, anamnesis and instrumental diagnosis data, clinical features of the course of the disease and the effectiveness of treatment were analyzed.<br><br>RESULTS: 121 children were treated for PPA (average age 73±41 months; Me=52.5 months), which is 0.4% of all hospitalized in the otorhinolaryngological department, 0.7% of the number of emergency hospitalizations, 0.8% of the number of hospitalized children with pharyngeal diseases, and 8.3% of the number of patients with pharyngeal abscess. Abscesses were more often localized in the upper pharynx, at the level of the I-II cervical vertebrae (49.6% of all observations); abscesses were found least often in the pharyngeal space (5.8%), there was no statistically significant difference between the right-sided and left-sided location: 47.9% and 46.2%, respectively. Surgical treatment was performed in 98 (81%) patients in the presence of an abscess capsule or an abscess diameter of more than 2 cm according to CT; the remaining 23 (19%) children were treated conservatively. The opening of the abscess was performed endopharyngeal, in the case of a pronounced deep lateral location of the abscess and its proximity to large blood vessels - with access through the tonsillar niche after preliminary tonsillectomy (19.4% of those operated).<br><br>CONCLUSION: The final diagnosis of parapharyngeal and retropharyngeal abscess can be established by contrast-enhanced computed tomography. Conservative treatment is indicated for a limited group of patients at the initial stages of the disease, most patients need surgical treatment.}, }
@article {pmid34946819, year = {2021}, author = {Filippenkov, IB and Stavchansky, VV and Denisova, AE and Valieva, LV and Remizova, JA and Mozgovoy, IV and Zaytceva, EI and Gubsky, LV and Limborska, SA and Dergunova, LV}, title = {Genome-Wide RNA-Sequencing Reveals Massive Circular RNA Expression Changes of the Neurotransmission Genes in the Rat Brain after Ischemia-Reperfusion.}, journal = {Genes}, volume = {12}, number = {12}, pages = {}, doi = {10.3390/genes12121870}, pmid = {34946819}, issn = {2073-4425}, support = {21-34-70048//Russian Foundation for Basic Research and Moscow city Government/ ; }, abstract = {Ischemic brain stroke is one of the most serious and socially significant diseases. In addition to messenger RNAs (mRNAs), encoding protein, the study of regulatory RNAs in ischemic has exceptional importance for the development of new strategies for neuroprotection. Circular RNAs (circRNAs) have a closed structure, predominantly brain-specific expression, and remain highly promising targets of research. They can interact with microRNAs (miRNAs), diminish their activity and thereby inhibit miRNA-mediated repression of mRNA. Genome-wide RNA-Seq analysis of the subcortical structures of the rat brain containing an ischemic damage focus and penumbra area revealed 395 circRNAs changed their expression significantly at 24 h after transient middle cerebral artery occlusion model (tMCAO) conditions. Furthermore, functional annotation revealed their association with neuroactive signaling pathways. It was found that about a third of the differentially expressed circRNAs (DECs) originate from genes whose mRNA levels also changed at 24 h after tMCAO. The other DECs originate from genes encoding non-regulated mRNAs under tMCAO conditions. In addition, bioinformatic analysis predicted a circRNA-miRNA-mRNA network which was associated with the neurotransmission signaling regulation. Our results show that such circRNAs can persist as potential miRNA sponges for the protection of mRNAs of neurotransmitter genes. The results expanded our views about the neurotransmission regulation in the rat brain after ischemia-reperfusion with circRNA action.}, }
@article {pmid34943946, year = {2021}, author = {Makarova, E and Kazantseva, A and Dubinina, A and Denisova, E and Jakovleva, T and Balybina, N and Bgatova, N and Baranov, K and Bazhan, N}, title = {Fibroblast Growth Factor 21 (FGF21) Administration Sex-Specifically Affects Blood Insulin Levels and Liver Steatosis in Obese Ay Mice.}, journal = {Cells}, volume = {10}, number = {12}, pages = {}, doi = {10.3390/cells10123440}, pmid = {34943946}, issn = {2073-4409}, support = {17-15-01036//Russian Science Foundation/ ; }, abstract = {FGF21 is a promising candidate for treating obesity, diabetes, and NAFLD; however, some of its pharmacological effects are sex-specific in mice with the Ay mutation that evokes melanocortin receptor 4 blockade, obesity, and hepatosteatosis. This suggests that the ability of FGF21 to correct melanocortin obesity may depend on sex. This study compares FGF21 action on food intake, locomotor activity, gene expression, metabolic characteristics, and liver state in obese Ay males and females. Ay mice were administered FGF21 for seven days, and metabolic parameters and gene expression in different tissues were assessed. Placebo-treated females were more obese than males and had lower levels of blood insulin and liver triglycerides, and higher expression of genes for insulin signaling in the liver, white adipose tissue (WAT) and muscles, and pro-inflammatory cytokines in the liver. FGF21 administration did not affect body weight, and increased food intake, locomotor activity, expression of Fgf21 and Ucp1 in brown fat and genes related to lipolysis and insulin action in WAT regardless of sex; however, it decreased hyperinsulinemia and hepatic lipid accumulation and increased muscle expression of Cpt1 and Irs1 only in males. Thus, FGF21's beneficial effects on metabolic disorders associated with melanocortin obesity are more pronounced in males.}, }
@article {pmid34942109, year = {2021}, author = {Bouaziz, M and Cheng, T and Minuti, A and Denisova, K and Barmettler, A}, title = {Shared Decision Making in Ophthalmology: A Scoping Review.}, journal = {American journal of ophthalmology}, volume = {}, number = {}, pages = {}, doi = {10.1016/j.ajo.2021.12.005}, pmid = {34942109}, issn = {1879-1891}, abstract = {PURPOSE: Shared decision making (SDM) has been associated with improved patient satisfaction and outcomes in both medical and surgical specialties, but its role in ophthalmology has not been systematically examined. Using a scoping review of the literature, the purpose of this study was to explore the characteristics, implementation, and outcomes of SDM in ophthalmology.<br><br>DESIGN: Scoping review of the literature.<br><br>METHODS: Searches were conducted in PubMed, Embase, Web of Science and the Cochrane Central Register of Controlled Trials (CENTRAL) through August of 2021 for SDM in ophthalmology. The resulting 1602 studies were screened by two independent reviewers with 57 full-text articles examined for inclusion of an ophthalmologic diagnosis, as well as discussion of SDM or patient decision aids (PDAs). Nineteen studies were eligible and qualitatively coded for 11 predetermined codes, which included patient outcomes, patient and physician requests for SDM, and methods of implementation.<br><br>RESULTS: Of the 19 included studies, all emphasized the value of SDM for ophthalmology and two studies reported improved patient outcomes. The most commonly examined topics were chronic ophthalmic diseases, such as cataracts, and glaucoma. Limitations to SDM implementation were also universally discussed, including patients' lack of disease knowledge, communication barriers, and time restrictions. Although PDAs are an effective tool to mitigate these, these have only been established for the subjects of cataracts and glaucoma.<br><br>CONCLUSION: SDM is a methodology for patient-centered care that is regarded as a potentially useful tool in the field of ophthalmology. However, significant barriers exist to its effective implementation. Evidence-based research on if and how these barriers should be attenuated, as well as the development of additional PDA's for different ophthalmic diseases, are needed.}, }
@article {pmid34937535, year = {2021}, author = {Zhur, KV and Trifonov, VA and Prokhortchouk, EB}, title = {Progress and Prospects in Epigenetic Studies of Ancient DNA.}, journal = {Biochemistry. Biokhimiia}, volume = {86}, number = {12}, pages = {1563-1571}, doi = {10.1134/S0006297921120051}, pmid = {34937535}, issn = {1608-3040}, abstract = {Development of technologies for high-throughput whole-genome sequencing and improvement of sample preparation techniques made it possible to study ancient DNA (aDNA) from archaeological samples over a million year old. The studies of aDNA have shed light on the history of human migration, replacement of populations, interbreeding of Cro-Magnons with Neanderthals and Denisovans, evolution of human pathogens, etc. Equally important is the possibility to investigate epigenetic modifications of ancient genomes, which has allowed to obtain previously inaccessible information on gene expression, nucleosome positioning, and DNA methylation. Analysis of methylation status of certain genomic sites can predict an individual's age at death and reconstruct some phenotypic features, as it was done for the Denisovan genome, and even to elucidate unfavorable environmental factors that had affected this archaic individual. In this review, we discuss current progress in epigenetic studies of aDNA, including methodological approaches and promising research directions in this field.}, }
@article {pmid34937505, year = {2021}, author = {Yarmolinskaya, M and Denisova, A and Tkachenko, N and Ivashenko, T and Bespalova, O and Tolibova, G and Tral, T}, title = {Vitamin D significance in pathogenesis of endometriosis.}, journal = {Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology}, volume = {37}, number = {sup1}, pages = {40-43}, doi = {10.1080/09513590.2021.2006516}, pmid = {34937505}, issn = {1473-0766}, abstract = {&#x200A;: Genital endometriosis (GE) is a widespread multifactorial disease thus making it necessary to carry on studying its pathogeny in order to work out target therapy techniques. Studying vitamin D role in GE pathogeny is a new promising research trend.<br><br>PATIENTS AND TECHNIQUE: 25(ОН)D level was determined in peripheral blood (PB) of 440 patients with GE and in peritoneal fluid (PF) - in 49 GE patients; the same test in PB was performed in 30 patients of the control group with the ovulatory menstrual cycle and no gynecologic pathology. 129 patients with GE, as well as 82 women of the control, underwent examination of vitamin D receptor (VDR) polymorphism gene in BsmI, FokI, and TaqI polymorphic locus. We examined vitamin D receptor expression in the eutopic and ectopic endometrium in 32 women with GE and in the endometrium of 20 women from the control group. We also compared the efficacy of combined therapy involving colecalciferol to the standard hormone modulating therapy.<br><br>RESULTS: It was established that the prevalent GE forms are characterized by lower 25(ОН)D levels both in PB and in PF. It was also found that G/G genotype of VDR BsmI gene polymorphic variant 1.9 times increases GE occurrence risk. VDR expression was authentically lower in the ectopic endometrium compared to the eutopic endometrium. Patients with GE show no VDR expression cyclic variations in the endometrium which is contrary to the control. Therapy in combination with colecalciferol promotes a more expressed decrease of endometriosis-associated pain syndrome and psycho-emotional stabilization of GE patients compared to the standard hormone modulating therapy.<br><br>CONCLUSION: Vitamin D deficiency plays a significant role in the pathogenesis of GE and Vit D may be applied as its targeted therapy.}, }
@article {pmid34919805, year = {2021}, author = {Natri, HM and Hudjashov, G and Jacobs, G and Kusuma, P and Saag, L and Darusallam, CC and Metspalu, M and Sudoyo, H and Cox, MP and Gallego Romero, I and Banovich, NE}, title = {Genetic architecture of gene regulation in Indonesian populations identifies QTLs associated with global and local ancestries.}, journal = {American journal of human genetics}, volume = {}, number = {}, pages = {}, doi = {10.1016/j.ajhg.2021.11.017}, pmid = {34919805}, issn = {1537-6605}, abstract = {Lack of diversity in human genomics limits our understanding of the genetic underpinnings of complex traits, hinders precision medicine, and contributes to health disparities. To map genetic effects on gene regulation in the underrepresented Indonesian population, we have integrated genotype, gene expression, and CpG methylation data from 115 participants across three island populations that capture the major sources of genomic diversity in the region. In a comparison with European datasets, we identify eQTLs shared between Indonesia and Europe as well as population-specific eQTLs that exhibit differences in allele frequencies and/or overall expression levels between populations. By combining local ancestry and archaic introgression inference with eQTLs and methylQTLs, we identify regulatory loci driven by modern Papuan ancestry as well as introgressed Denisovan and Neanderthal variation. GWAS colocalization connects QTLs detected here to hematological traits, and further comparison with European datasets reflects the poor overall transferability of GWAS statistics across diverse populations. Our findings illustrate how population-specific genetic architecture, local ancestry, and archaic introgression drive variation in gene regulation across genetically distinct and in admixed populations and highlight the need for performing association studies on non-European populations.}, }
@article {pmid34897770, year = {2021}, author = {Shunatova, N and Serova, K and Denisova, S and Shchenkov, S and Ostrovsky, A}, title = {Small, but smart: fine structure of an avicularium in Dendrobeania fruticosa (Bryozoa: Cheilostomata).}, journal = {Journal of morphology}, volume = {}, number = {}, pages = {}, doi = {10.1002/jmor.21436}, pmid = {34897770}, issn = {1097-4687}, abstract = {Bryozoans are small benthic suspension-feeding colonial animals. Among this phylum, there are representatives showing a lesser or greater degree of polymorphism, and the most common type of polymorphic zooids is the avicularium. Here we present a detailed description of the bird's-head shaped avicularium in Dendrobeania fruticosa. The body cavity of the avicularium demonstrates an acoelomate condition: along the cystid walls, there is neither the layer of extracellular matrix towards the epidermis, nor coelomic lining. However, a layer of an extracellular matrix and epithelialized cells lie under the epidermis of the tentacle sheath. Probably, such construction helps the tentacle sheath to acquire some rigidity - it is the only region of the body wall without an ectocyst. We did not find typical funicular strands in the avicularium, but there is a delicate mesh composed of stellate cells with thin and long projections, which sometimes isolate the spaces filled with a heterogeneous matrix. The proximal ends of the adductors, abductors, and polypide retractors are attached to the body wall via typical epidermal tendon cells, which possess numerous bundles of tonofilaments. The distal ends of the abductors and adductors attach to the frontal membrane or upper vestibular membrane, respectively. The inner organic layer of the ectocyst in these regions forms large protrusions, from which numerous thin outgrowths branch off. We suggest them to be a functional analogue of apodemes and apodemal filaments in arthropods. "Apodemal" tendon cells have long and thin projections that line the outgrowths of the ectocyst and surround the distal ends of the muscle cells. At these sites, "apodemal" tendon cells possess numerous tonofilaments. The vestigial polypide includes the tentacle sheath, rudimentary lophophore, cerebral ganglion, and polypide retractors. The sensory part of 5HT-positive cells of the frontal membrane is dendrite-shaped and embedded in the inner organic layer of the ectocyst. This article is protected by copyright. All rights reserved.}, }
@article {pmid34872977, year = {2021}, author = {Hubert, L and Paganini, J and Picard, C and Chiaroni, J and Abi-Rached, L and Pontarotti, P and Di Cristofaro, J}, title = {HLA-H*02:07 Is a Membrane-Bound Ligand of Denisovan Origin That Protects against Lysis by Activated Immune Effectors.}, journal = {Journal of immunology (Baltimore, Md. : 1950)}, volume = {}, number = {}, pages = {}, doi = {10.4049/jimmunol.2100358}, pmid = {34872977}, issn = {1550-6606}, abstract = {The biological relevance of genes initially categorized as "pseudogenes" is slowly emerging, notably in innate immunity. In the HLA region on chromosome 6, HLA-H is one such pseudogene; yet, it is transcribed, and its variation is associated with immune properties. Furthermore, two HLA-H alleles, H*02:07 and H*02:14, putatively encode a complete, membrane-bound HLA protein. Here we thus hypothesized that HLA-H contributes to immune homeostasis similarly to tolerogenic molecules HLA-G, -E, and -F. We tested if HLA-H*02:07 encodes a membrane-bound protein that can inhibit the cytotoxicity of effector cells. We used an HLA-null human erythroblast cell line transduced with HLA-H*02:07 cDNA to demonstrate that HLA-H*02:07 encodes a membrane-bound protein. Additionally, using a cytotoxicity assay, our results support that K562 HLA-H*02:07 inhibits human effector IL-2-activated PBMCs and human IL-2-independent NK92-MI cell line activity. Finally, through in silico genotyping of the Denisovan genome and haplotypic association with Denisovan-derived HLA-A*11, we also show that H*02:07 is of archaic origin. Hence, admixture with archaic humans brought a functional HLA-H allele into modern European and Asian populations.}, }
@article {pmid34863581, year = {2021}, author = {Zhang, P and Zhang, X and Zhang, X and Gao, X and Huerta-Sanchez, E and Zwyns, N}, title = {Denisovans and Homo sapiens on the Tibetan Plateau: dispersals and adaptations.}, journal = {Trends in ecology &amp; evolution}, volume = {}, number = {}, pages = {}, doi = {10.1016/j.tree.2021.11.004}, pmid = {34863581}, issn = {1872-8383}, abstract = {Recent archaeological discoveries suggest that both archaic Denisovans and Homo sapiens occupied the Tibetan Plateau earlier than expected. Genetic studies show that a pulse of Denisovan introgression was involved in the adaptation of Tibetan populations to high-altitude hypoxia. These findings challenge the traditional view that the plateau was one of the last places on earth colonized by H. sapiens and warrant a reappraisal of the population history of this highland. Here, we integrate archaeological and genomic evidence relevant to human dispersal, settlement, and adaptation in the region. We propose two testable models to address the peopling of the plateau in the broader context of H. sapiens dispersal and their encounters with Denisovans in Asia.}, }
@article {pmid34851566, year = {2021}, author = {Koloda, YA and Denisova, YV and Podzolkova, NM}, title = {Genetic polymorphisms of reproductive hormones and their receptors in assisted reproduction technology for patients with polycystic ovary syndrome.}, journal = {Drug metabolism and personalized therapy}, volume = {}, number = {}, pages = {}, doi = {10.1515/dmpt-2021-0123}, pmid = {34851566}, issn = {2363-8915}, abstract = {Polycystic ovary syndrome (PCOS) is one of the most common endocrinopathies in women of childbearing, which is defined by the accumulation of multiple, small fluid-filled ovarian cysts without the selection of a single dominant follicle. Most PCOS phenotypes are characterized by the absence of spontaneous ovulation, resistance toward ovulation inductors, the production of a large immature oocytes number, and the high prevalence of ovarian hyperstimulation syndrome, resulting in reduced assisted reproductive technologies (ART) programs effectiveness. The review analyses current data about the relationship between polymorphism genotypes of KISS genes, follicle stimulating hormone (FSH), luteinizing hormone (LH), anti-Müllerian hormone (AMH) and their receptors genes, gonadotropin-releasing hormone (GnRH), estrogen, and progesterone receptors genes, the PCOS risk and the features of ovarian response to stimulation during ART cycles. The use of single nucleotide polymorphisms (SNPs) as prognostic markers of ART programs outcomes would provide a personalized approach to the drugs and doses choice for ovarian stimulation and significantly increase the chance of pregnancy.}, }
@article {pmid34824388, year = {2021}, author = {Brown, S and Massilani, D and Kozlikin, MB and Shunkov, MV and Derevianko, AP and Stoessel, A and Jope-Street, B and Meyer, M and Kelso, J and Pääbo, S and Higham, T and Douka, K}, title = {The earliest Denisovans and their cultural adaptation.}, journal = {Nature ecology &amp; evolution}, volume = {}, number = {}, pages = {}, pmid = {34824388}, issn = {2397-334X}, support = {715069//EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)/ ; 715069//EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)/ ; 694707//EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)/ ; 715069//EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)/ ; 694707//EC | EC Seventh Framework Programm | FP7 Ideas: European Research Council (FP7-IDEAS-ERC - Specific Programme: "Ideas" Implementing the Seventh Framework Programme of the European Community for Research, Technological Development and Demonstration Activities (2007 to 2013))/ ; 324139//EC | EC Seventh Framework Programm | FP7 Ideas: European Research Council (FP7-IDEAS-ERC - Specific Programme: "Ideas" Implementing the Seventh Framework Programme of the European Community for Research, Technological Development and Demonstration Activities (2007 to 2013))/ ; 18-09-40100//Russian Foundation for Basic Research (RFBR)/ ; 18-09-40100//Russian Foundation for Basic Research (RFBR)/ ; 18-09-40100//Russian Foundation for Basic Research (RFBR)/ ; }, abstract = {Since the initial identification of the Denisovans a decade ago, only a handful of their physical remains have been discovered. Here we analysed ~3,800 non-diagnostic bone fragments using collagen peptide mass fingerprinting to locate new hominin remains from Denisova Cave (Siberia, Russia). We identified five new hominin bones, four of which contained sufficient DNA for mitochondrial analysis. Three carry mitochondrial DNA of the Denisovan type and one was found to carry mtDNA of the Neanderthal type. The former come from the same archaeological layer near the base of the cave's sequence and are the oldest securely dated evidence of Denisovans at 200 ka (thousand years ago) (205-192 ka at 68.2% or 217-187 ka at 95% probability). The stratigraphic context in which they were located contains a wealth of archaeological material in the form of lithics and faunal remains, allowing us to determine the material culture associated with these early hominins and explore their behavioural and environmental adaptations. The combination of bone collagen fingerprinting and genetic analyses has so far more-than-doubled the number of hominin bones at Denisova Cave and has expanded our understanding of Denisovan and Neanderthal interactions, as well as their archaeological signatures.}, }
@article {pmid34795445, year = {2021}, author = {Liston, A and Humblet-Baron, S and Duffy, D and Goris, A}, title = {Human immune diversity: from evolution to modernity.}, journal = {Nature immunology}, volume = {}, number = {}, pages = {}, pmid = {34795445}, issn = {1529-2916}, support = {874707//EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020)/ ; 874707//EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020)/ ; ANR-10-LABX-69-01//Agence Nationale de la Recherche (French National Research Agency)/ ; G.07334.15//Fonds Wetenschappelijk Onderzoek (Research Foundation Flanders)/ ; }, abstract = {The extreme diversity of the human immune system, forged and maintained throughout evolutionary history, provides a potent defense against opportunistic pathogens. At the same time, this immune variation is the substrate upon which a plethora of immune-associated diseases develop. Genetic analysis suggests that thousands of individually weak loci together drive up to half of the observed immune variation. Intense selection maintains this genetic diversity, even selecting for the introgressed Neanderthal or Denisovan alleles that have reintroduced variation lost during the out-of-Africa migration. Variations in age, sex, diet, environmental exposure, and microbiome each potentially explain the residual variation, with proof-of-concept studies demonstrating both plausible mechanisms and correlative associations. The confounding interaction of many of these variables currently makes it difficult to assign definitive contributions. Here, we review the current state of play in the field, identify the key unknowns in the causality of immune variation, and identify the multidisciplinary pathways toward an improved understanding.}, }
@article {pmid34782887, year = {2021}, author = {Denisova, EI and Savinkova, MM and Makarova, EN}, title = {Influence of leptin administration to pregnant female mice on obesity development, taste preferences, and gene expression in the liver and muscles of their male and female offspring.}, journal = {Vavilovskii zhurnal genetiki i selektsii}, volume = {25}, number = {6}, pages = {669-676}, doi = {10.18699/VJ21.076}, pmid = {34782887}, issn = {2500-0462}, abstract = {. The consumption of food rich in sugar and fat provokes obesity. Prenatal conditions have an impact on taste preferences and metabolism in the adult offspring, and this impact may manifest differently in different sexes. An increase in blood leptin level in pregnant females reduces the risk of obesity and insulin resistance in the offspring, although the mechanisms mediating this effect are unknown. Neither is it known whether maternal leptin affects taste preferences. In this study, we investigated the effect of leptin administration to pregnant mice on the development of diet-induced obesity, food choice, and gene expression in the liver and muscles of the offspring with regard to sex. Leptin was administered to female mice on days 11, 12, and 13 of pregnancy. In male and female offspring, growth rate and intake of standard chow after weaning, obesity development, gene expression in the liver and muscles, and food choice when kept on a high-calorie diet (standard chow, lard, sweet cookies) were recorded. Leptin administration to pregnant females reduced body weight in the female offspring fed on the standard diet. When the offspring were given a high-calorie diet, leptin administration inhibited obesity development and reduced the consumption of cookies only in males. It also increased the consumption of standard chow and the mRNA levels of genes for the insulin receptor and glucose transporter type 4 in the muscles of both male and female offspring. The results demonstrate that an increase in blood leptin levels in pregnant females has a sex-specif ic effect on the metabolism of the offspring increasing resistance to obesity only in male offspring. The mechanism underlying this effect includes a shift in food preference in favor of a balanced diet and maintenance of insulin sensitivity in muscle tissues.}, }
@article {pmid34771075, year = {2021}, author = {Grin, IR and Mechetin, GV and Kasymov, RD and Diatlova, EA and Yudkina, AV and Shchelkunov, SN and Gileva, IP and Denisova, AA and Stepanov, GA and Chilov, GG and Zharkov, DO}, title = {A New Class of Uracil-DNA Glycosylase Inhibitors Active against Human and Vaccinia Virus Enzyme.}, journal = {Molecules (Basel, Switzerland)}, volume = {26}, number = {21}, pages = {}, doi = {10.3390/molecules26216668}, pmid = {34771075}, issn = {1420-3049}, support = {17-14-01190//Russian Science Foundation/ ; 0245-2021-0002//Ministry of Science and Higher Education, Russia/ ; FSUS-2020-0035//Ministry of Science and Higher Education, Russia/ ; 21-04-011//Academic Fund Program at the HSE University in 2021/ ; }, abstract = {Uracil-DNA glycosylases are enzymes that excise uracil bases appearing in DNA as a result of cytosine deamination or accidental dUMP incorporation from the dUTP pool. The activity of Family 1 uracil-DNA glycosylase (UNG) activity limits the efficiency of antimetabolite drugs and is essential for virulence in some bacterial and viral infections. Thus, UNG is regarded as a promising target for antitumor, antiviral, antibacterial, and antiprotozoal drugs. Most UNG inhibitors presently developed are based on the uracil base linked to various substituents, yet new pharmacophores are wanted to target a wide range of UNGs. We have conducted virtual screening of a 1,027,767-ligand library and biochemically screened the best hits for the inhibitory activity against human and vaccinia virus UNG enzymes. Although even the best inhibitors had IC50 ≥ 100 μM, they were highly enriched in a common fragment, tetrahydro-2,4,6-trioxopyrimidinylidene (PyO3). In silico, PyO3 preferably docked into the enzyme's active site, and in kinetic experiments, the inhibition was better consistent with the competitive mechanism. The toxicity of two best inhibitors for human cells was independent of the presence of methotrexate, which is consistent with the hypothesis that dUMP in genomic DNA is less toxic for the cell than strand breaks arising from the massive removal of uracil. We conclude that PyO3 may be a novel pharmacophore with the potential for development into UNG-targeting agents.}, }
@article {pmid34757478, year = {2021}, author = {Derenko, M and Denisova, G and Dambueva, I and Malyarchuk, B and Bazarov, B}, title = {Mitogenomics of modern Mongolic-speaking populations.}, journal = {Molecular genetics and genomics : MGG}, volume = {}, number = {}, pages = {}, pmid = {34757478}, issn = {1617-4623}, support = {14.W03.31.0016 "Dynamics of Peoples//Government of the Russian Federation/ ; Empires in the History of Inner Asia"//Government of the Russian Federation/ ; }, abstract = {Here, we present a comprehensive data set of 489 complete mitogenomes (211 of which are new) from four Mongolic-speaking populations (Mongols, Barghuts, Khamnigans, and Buryats) to investigate their matrilineal genetic structure, ancestry and relationship with other ethnic groups. We show that along with very high levels of genetic diversity and lack of genetic differentiation, Mongolic-speaking populations exhibit strong genetic resemblance to East Asian populations of Chinese, Japanese, and Uyghurs. Phylogeographic analysis of complete mitogenomes reveals the presence of different components in the gene pools of modern Mongolic-speaking populations-the main East Eurasian component is represented by mtDNA lineages of East Asian, Siberian and autochthonous (the Baikal region/Mongolian) ancestry, whereas the less pronounced West Eurasian component can be ascribed to Europe and West Asia/Caucasus. We also observed that up to one third of the mtDNA subhaplogroups identified in Mongolic-speaking populations can be considered as Mongolic-specific with the coalescence age of most of them not exceeding 1.7 kya. This coincides well with the population size growth which started around 1.1 kya and is detectable only in the Bayesian Skyline Plot constructed based on Mongolic-specific mitogenomes. Our data suggest that the genetic structure established during the Mongol empire is still retained in present-day Mongolic-speaking populations.}, }
@article {pmid34716352, year = {2021}, author = {Di Pietro, L and Barba, M and Palacios, D and Tiberio, F and Prampolini, C and Baranzini, M and Parolini, O and Arcovito, A and Lattanzi, W}, title = {Shaping modern human skull through epigenetic, transcriptional and post-transcriptional regulation of the RUNX2 master bone gene.}, journal = {Scientific reports}, volume = {11}, number = {1}, pages = {21316}, pmid = {34716352}, issn = {2045-2322}, support = {Linea D1 - 2018//Università Cattolica del Sacro Cuore/ ; Linea D1 - 2017//Università Cattolica del Sacro Cuore/ ; }, abstract = {RUNX2 encodes the master bone transcription factor driving skeletal development in vertebrates, and playing a specific role in craniofacial and skull morphogenesis. The anatomically modern human (AMH) features sequence changes in the RUNX2 locus compared with archaic hominins' species. We aimed to understand how these changes may have contributed to human skull globularization occurred in recent evolution. We compared in silico AMH and archaic hominins' genomes, and used mesenchymal stromal cells isolated from skull sutures of craniosynostosis patients for in vitro functional assays. We detected 459 and 470 nucleotide changes in noncoding regions of the AMH RUNX2 locus, compared with the Neandertal and Denisovan genomes, respectively. Three nucleotide changes in the proximal promoter were predicted to alter the binding of the zinc finger protein Znf263 and long-distance interactions with other cis-regulatory regions. By surface plasmon resonance, we selected nucleotide substitutions in the 3'UTRs able to affect miRNA binding affinity. Specifically, miR-3150a-3p and miR-6785-5p expression inversely correlated with RUNX2 expression during in vitro osteogenic differentiation. The expression of two long non-coding RNAs, AL096865.1 and RUNX2-AS1, within the same locus, was modulated during in vitro osteogenic differentiation and correlated with the expression of specific RUNX2 isoforms. Our data suggest that RUNX2 may have undergone adaptive phenotypic evolution caused by epigenetic and post-transcriptional regulatory mechanisms, which may explain the delayed suture fusion leading to the present-day globular skull shape.}, }
@article {pmid34716342, year = {2021}, author = {Yuan, K and Ni, X and Liu, C and Pan, Y and Deng, L and Zhang, R and Gao, Y and Ge, X and Liu, J and Ma, X and Lou, H and Wu, T and Xu, S}, title = {Refining models of archaic admixture in Eurasia with ArchaicSeeker 2.0.}, journal = {Nature communications}, volume = {12}, number = {1}, pages = {6232}, pmid = {34716342}, issn = {2041-1723}, support = {32030020//National Natural Science Foundation of China (National Science Foundation of China)/ ; }, abstract = {We developed a method, ArchaicSeeker 2.0, to identify introgressed hominin sequences and model multiple-wave admixture. The new method enabled us to discern two waves of introgression from both Denisovan-like and Neanderthal-like hominins in present-day Eurasian populations and an ancient Siberian individual. We estimated that an early Denisovan-like introgression occurred in Eurasia around 118.8-94.0 thousand years ago (kya). In contrast, we detected only one single episode of Denisovan-like admixture in indigenous peoples eastern to the Wallace-Line. Modeling ancient admixtures suggested an early dispersal of modern humans throughout Asia before the Toba volcanic super-eruption 74 kya, predating the initial peopling of Asia as proposed by the traditional Out-of-Africa model. Survived archaic sequences are involved in various phenotypes including immune and body mass (e.g., ZNF169), cardiovascular and lung function (e.g., HHAT), UV response and carbohydrate metabolism (e.g., HYAL1/HYAL2/HYAL3), while "archaic deserts" are enriched with genes associated with skin development and keratinization.}, }
@article {pmid34714000, year = {2021}, author = {Dzhafarov, VM and Guzeeva, AS and Amelina, EV and Khalepa, AA and Dmitriev, AB and Denisova, NP and Rzaev, DA}, title = {[Invasive EEG for temporal lobe epilepsy: selection of technique].}, journal = {Zhurnal voprosy neirokhirurgii imeni N. N. Burdenko}, volume = {85}, number = {5}, pages = {23-29}, doi = {10.17116/neiro20218505123}, pmid = {34714000}, issn = {0042-8817}, abstract = {BACKGROUND: Non-invasive EEG reveals epileptogenic zone in 70% of patients. In other cases, invasive EEG monitoring is indicated. Various implantation strategies and techniques of intracranial EEG (icEEG) potentially provide different outcomes. Choosing the optimal icEEG technique may be challenging.<br><br>OBJECTIVE: To analyze the results of icEEG in adults with temporal lobe epilepsy and to determine the algorithm for selection of optimal invasive EEG technique.<br><br>MATERIAL AND METHODS: The study included 82 patients with temporal lobe epilepsy who underwent invasive EEG. Effectiveness of invasive EEG was determined by detection of epileptogenic zone and post-resection outcomes. Postoperative results were analyzed throughout more than 6-month follow-up period using the Engel grading system. Statistical analysis was conducted using the Fisher's exact test.<br><br>RESULTS: Epileptogenic zone was revealed in 72 (88%) cases. Invasive EEG was supplemented by another modality in 3 (4%) patients. Mean follow-up period after resection was 17 months in 45 patients. Favorable outcomes were achieved in 31 (69%) cases. Statistical analysis showed that identification of epileptogenic zone depends existing of lesion and symptoms of seizures. Selection algorithm for optimal technique of invasive EEG was determined considering own results and literature data.<br><br>CONCLUSION: Invasive EEG results and post-resection outcomes demonstrated favorable efficacy of original algorithm. The last one may be used in decision-making on optimal technique of invasive EEG in adults with temporal lobe epilepsy.}, }
@article {pmid34692055, year = {2020}, author = {,  and He, Y and Lou, H and Cui, C and Deng, L and Gao, Y and Zheng, W and Guo, Y and Wang, X and Ning, Z and Li, J and Li, B and Bai, C and ,  and ,  and ,  and ,  and ,  and Liu, S and Wu, T and Xu, S and Qi, X and Su, B}, title = {De novo assembly of a Tibetan genome and identification of novel structural variants associated with high-altitude adaptation.}, journal = {National science review}, volume = {7}, number = {2}, pages = {391-402}, doi = {10.1093/nsr/nwz160}, pmid = {34692055}, issn = {2053-714X}, abstract = {Structural variants (SVs) may play important roles in human adaptation to extreme environments such as high altitude but have been under-investigated. Here, combining long-read sequencing with multiple scaffolding techniques, we assembled a high-quality Tibetan genome (ZF1), with a contig N50 length of 24.57 mega-base pairs (Mb) and a scaffold N50 length of 58.80 Mb. The ZF1 assembly filled 80 remaining N-gaps (0.25 Mb in total length) in the reference human genome (GRCh38). Markedly, we detected 17 900 SVs, among which the ZF1-specific SVs are enriched in GTPase activity that is required for activation of the hypoxic pathway. Further population analysis uncovered a 163-bp intronic deletion in the MKL1 gene showing large divergence between highland Tibetans and lowland Han Chinese. This deletion is significantly associated with lower systolic pulmonary arterial pressure, one of the key adaptive physiological traits in Tibetans. Moreover, with the use of the high-quality de novo assembly, we observed a much higher rate of genome-wide archaic hominid (Altai Neanderthal and Denisovan) shared non-reference sequences in ZF1 (1.32%-1.53%) compared to other East Asian genomes (0.70%-0.98%), reflecting a unique genomic composition of Tibetans. One such archaic hominid shared sequence-a 662-bp intronic insertion in the SCUBE2 gene-is enriched and associated with better lung function (the FEV1/FVC ratio) in Tibetans. Collectively, we generated the first high-resolution Tibetan reference genome, and the identified SVs may serve as valuable resources for future evolutionary and medical studies.}, }
@article {pmid34615474, year = {2021}, author = {Kraef, C and Bentzon, A and Panteleev, A and Skrahina, A and Bolokadze, N and Tetradov, S and Podlasin, R and Karpov, I and Borodulina, E and Denisova, E and Azina, I and Lundgren, J and Johansen, IS and Mocroft, A and Podlekareva, D and Kirk, O and , }, title = {Delayed diagnosis of tuberculosis in persons living with HIV in Eastern Europe: associated factors and effect on mortality-a multicentre prospective cohort study.}, journal = {BMC infectious diseases}, volume = {21}, number = {1}, pages = {1038}, pmid = {34615474}, issn = {1471-2334}, abstract = {BACKGROUND: Early diagnosis of tuberculosis (TB) is important to reduce transmission, morbidity and mortality in people living with HIV (PLWH).<br><br>METHODS: PLWH with a diagnosis of TB were enrolled from HIV and TB clinics in Eastern Europe and followed until 24 months. Delayed diagnosis was defined as duration of TB symptoms (cough, weight-loss or fever) for ≥ 1 month before TB diagnosis. Risk factors for delayed TB diagnosis were assessed using multivariable logistic regression. The effect of delayed diagnosis on mortality was assessed using Kaplan-Meier estimates and Cox models.<br><br>FINDINGS: 480/740 patients (64.9%; 95% CI 61.3-68.3%) experienced a delayed diagnosis. Age ≥ 50 years (vs. < 50 years, aOR = 2.51; 1.18-5.32; p = 0.016), injecting drug use (IDU) (vs. non-IDU aOR = 1.66; 1.21-2.29; p = 0.002), being ART naïve (aOR = 1.77; 1.24-2.54; p = 0.002), disseminated TB (vs. pulmonary TB, aOR = 1.56, 1.10-2.19, p = 0.012), and presenting with weight loss (vs. no weight loss, aOR = 1.63; 1.18-2.24; p = 0.003) were associated with delayed diagnosis. PLWH with a delayed diagnosis were at 36% increased risk of death (hazard ratio = 1.36; 1.04-1.77; p = 0.023, adjusted hazard ratio 1.27; 0.95-1.70; p = 0.103).<br><br>CONCLUSION: Nearly two thirds of PLWH with TB in Eastern Europe had a delayed TB diagnosis, in particular those of older age, people who inject drugs, ART naïve, with disseminated disease, and presenting with weight loss. Patients with delayed TB diagnosis were subsequently at higher risk of death in unadjusted analysis. There is a need for optimisation of the current TB diagnostic cascade and HIV care in PLWH in Eastern Europe.}, }
@article {pmid34575702, year = {2021}, author = {Sobolev, V and Soboleva, A and Denisova, E and Denieva, M and Dvoryankova, E and Suleymanov, E and Zhukova, OV and Potekaev, N and Korsunskaya, I and Mezentsev, A}, title = {Differential Expression of Estrogen-Responsive Genes in Women with Psoriasis.}, journal = {Journal of personalized medicine}, volume = {11}, number = {9}, pages = {}, doi = {10.3390/jpm11090925}, pmid = {34575702}, issn = {2075-4426}, abstract = {In women, the flow of psoriasis is influenced by each phase of a woman's life cycle. According to previous findings, significant changes in the levels of sex hormones affect the severity of the disease. Aim: The aim of this study was to identify the estrogen-responsive genes that could be responsible for the exacerbation of psoriasis in menopausal women. Methods: Skin samples of lesional skin donated by psoriasis patients (n = 5) were compared with skin samples of healthy volunteers (n = 5) using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The set of differentially expressed proteins was subjected to protein ontology analysis to identify differentially expressed estrogen-responsive proteins. The expression of discovered proteins was validated by qPCR and ELISA on four groups of female participants. The first group included ten psoriasis patients without menopause; the second included eleven postmenopausal patients; the third included five healthy volunteers without menopause; and the fourth included six postmenopausal volunteers. Moreover, the participants' blood samples were used to assess the levels of estradiol, progesterone, and testosterone. Results: We found that the levels of estradiol and progesterone were significantly lower and the levels of testosterone were significantly higher in the blood of patients compared to the control. The protein ontology analysis of LC-MS/MS data identified six proteins, namely HMOX1, KRT19, LDHA, HSPD1, MAPK1, and CA2, differentially expressed in the lesional skin of female patients compared to male patients. ELISA and qPCR experiments confirmed differential expression of the named proteins and their mRNA. The genes encoding the named proteins were differentially expressed in patients compared to volunteers. However, KRT19 and LDHA were not differentially expressed when we compared patients with and without menopause. All genes, except MAPK1, were differentially expressed in patients with menopause compared to the volunteers with menopause. HMOX1, KRT19, HSPD1, and LDHA were differentially expressed in patients without menopause compared to the volunteers without menopause. However, no significant changes were found when we compared healthy volunteers with and without menopause. Conclusion: Our experiments discovered a differential expression of six estrogen-controlled genes in the skin of female patients. Identification of these genes and assessment of the changes in their expression provide insight into the biological effects of estrogen in lesional skin. The results of proteomic analysis are available via ProteomeXchange with identifier PXD021673.}, }
@article {pmid34449702, year = {2021}, author = {Deņisova, A and Pilmane, M and Eņģelis, A and Pētersons, A}, title = {Gallbladder Interleukins in Children with Calculous Cholecystitis.}, journal = {Pediatric reports}, volume = {13}, number = {3}, pages = {470-482}, doi = {10.3390/pediatric13030054}, pmid = {34449702}, issn = {2036-749X}, abstract = {Calculous cholecystitis connects to inflammation and various complications. It is a common disease in the paediatric population, yet it is still uncertain how inflammation factors are involved in its morphopathogenesis. Twenty calculous cholecystitis surgery tissue samples were obtained from 20 children. As a control, seven unaffected gallbladders were used. Tissues were immunohistochemically stained for IL-1α, IL-4, IL-6, IL-7, IL-8, IL-10, and IL-17A, and the slides were inspected by light microscopy. To evaluate statistical differences and correlations between interleukins, Mann-Whitney U and Spearman's tests were used. Statistically significant difference between patient and control gallbladder epithelium was for IL-1α and IL-17A, but connective tissue-IL-1α, IL-4, IL-6, IL-7, IL-8, and IL-17A positive structures. A strong positive correlation in patients was detected between epithelial IL-1α and IL-1α in connective tissue, epithelial IL-6 and IL-7, IL-6 and IL-17A, IL-7 and IL-10, IL-7 and IL-17A, as well as between IL-6 and IL-7, IL-7 and IL-10 in connective tissue. The increase of IL-1α, IL-4, IL-6, IL-7, IL-8 and IL-17A positive structures suggests their role in the morphopathogenesis of calculous cholecystitis. The correlations between interleukins in epithelium and in connective tissues prove that the epithelial barrier function and inflammatory response in deeper layers are sustained through intercellular signalling pathways.}, }
@article {pmid34445309, year = {2021}, author = {Sobolev, V and Nesterova, A and Soboleva, A and Mezentsev, A and Dvoriankova, E and Piruzyan, A and Denisova, E and Melnichenko, O and Korsunskaya, I}, title = {Analysis of PPARγ Signaling Activity in Psoriasis.}, journal = {International journal of molecular sciences}, volume = {22}, number = {16}, pages = {}, doi = {10.3390/ijms22168603}, pmid = {34445309}, issn = {1422-0067}, abstract = {In our previous work, we built the model of PPARγ dependent pathways involved in the development of the psoriatic lesions. Peroxisome proliferator-activated receptor gamma (PPARγ) is a nuclear receptor and transcription factor which regulates the expression of many proinflammatory genes. We tested the hypothesis that low levels of PPARγ expression promote the development of psoriatic lesions triggering the IL17-related signaling cascade. Skin samples of normally looking and lesional skin donated by psoriasis patients and psoriatic CD3+ Tcells samples (n = 23) and samples of healthy CD3+ T cells donated by volunteers (n = 10) were analyzed by real-time PCR, ELISA and immunohistochemistry analysis. We found that the expression of PPARγ is downregulated in human psoriatic skin and laser treatment restores the expression. The expression of IL17, STAT3, FOXP3, and RORC in psoriatic skin before and after laser treatment were correlated with PPARγ expression according to the reconstructed model of PPARγ pathway in psoriasis.In conclusion, we report that PPARγ weakens the expression of genes that contribute in the development of psoriatic lesion. Our data show that transcriptional regulation of PPARγ expression by FOSL1 and by STAT3/FOSL1 feedback loop may be central in the psoriatic skin and T-cells.}, }
@article {pmid34433944, year = {2021}, author = {Carlhoff, S and Duli, A and Nägele, K and Nur, M and Skov, L and Sumantri, I and Oktaviana, AA and Hakim, B and Burhan, B and Syahdar, FA and McGahan, DP and Bulbeck, D and Perston, YL and Newman, K and Saiful, AM and Ririmasse, M and Chia, S and Hasanuddin,  and Pulubuhu, DAT and Suryatman,  and Supriadi,  and Jeong, C and Peter, BM and Prüfer, K and Powell, A and Krause, J and Posth, C and Brumm, A}, title = {Genome of a middle Holocene hunter-gatherer from Wallacea.}, journal = {Nature}, volume = {596}, number = {7873}, pages = {543-547}, pmid = {34433944}, issn = {1476-4687}, abstract = {Much remains unknown about the population history of early modern humans in southeast Asia, where the archaeological record is sparse and the tropical climate is inimical to the preservation of ancient human DNA1. So far, only two low-coverage pre-Neolithic human genomes have been sequenced from this region. Both are from mainland Hòabìnhian hunter-gatherer sites: Pha Faen in Laos, dated to 7939-7751 calibrated years before present (yr cal BP; present taken as AD 1950), and Gua Cha in Malaysia (4.4-4.2 kyr cal BP)1. Here we report, to our knowledge, the first ancient human genome from Wallacea, the oceanic island zone between the Sunda Shelf (comprising mainland southeast Asia and the continental islands of western Indonesia) and Pleistocene Sahul (Australia-New Guinea). We extracted DNA from the petrous bone of a young female hunter-gatherer buried 7.3-7.2 kyr cal BP at the limestone cave of Leang Panninge2 in South Sulawesi, Indonesia. Genetic analyses show that this pre-Neolithic forager, who is associated with the 'Toalean' technocomplex3,4, shares most genetic drift and morphological similarities with present-day Papuan and Indigenous Australian groups, yet represents a previously unknown divergent human lineage that branched off around the time of the split between these populations approximately 37,000 years ago5. We also describe Denisovan and deep Asian-related ancestries in the Leang Panninge genome, and infer their large-scale displacement from the region today.}, }
@article {pmid34388371, year = {2021}, author = {Larena, M and McKenna, J and Sanchez-Quinto, F and Bernhardsson, C and Ebeo, C and Reyes, R and Casel, O and Huang, JY and Hagada, KP and Guilay, D and Reyes, J and Allian, FP and Mori, V and Azarcon, LS and Manera, A and Terando, C and Jamero, L and Sireg, G and Manginsay-Tremedal, R and Labos, MS and Vilar, RD and Latiph, A and Saway, RL and Marte, E and Magbanua, P and Morales, A and Java, I and Reveche, R and Barrios, B and Burton, E and Salon, JC and Kels, MJT and Albano, A and Cruz-Angeles, RB and Molanida, E and Granehäll, L and Vicente, M and Edlund, H and Loo, JH and Trejaut, J and Ho, SYW and Reid, L and Lambeck, K and Malmström, H and Schlebusch, C and Endicott, P and Jakobsson, M}, title = {Philippine Ayta possess the highest level of Denisovan ancestry in the world.}, journal = {Current biology : CB}, volume = {}, number = {}, pages = {}, doi = {10.1016/j.cub.2021.07.022}, pmid = {34388371}, issn = {1879-0445}, abstract = {Multiple lines of evidence show that modern humans interbred with archaic Denisovans. Here, we report an account of shared demographic history between Australasians and Denisovans distinctively in Island Southeast Asia. Our analyses are based on ∼2.3 million genotypes from 118 ethnic groups of the Philippines, including 25 diverse self-identified Negrito populations, along with high-coverage genomes of Australopapuans and Ayta Magbukon Negritos. We show that Ayta Magbukon possess the highest level of Denisovan ancestry in the world-∼30%-40% greater than that of Australians and Papuans-consistent with an independent admixture event into Negritos from Denisovans. Together with the recently described Homo luzonensis, we suggest that there were multiple archaic species that inhabited the Philippines prior to the arrival of modern humans and that these archaic groups may have been genetically related. Altogether, our findings unveil a complex intertwined history of modern and archaic humans in the Asia-Pacific region, where distinct Islander Denisovan populations differentially admixed with incoming Australasians across multiple locations and at various points in time.}, }
@article {pmid34326389, year = {2021}, author = {Brown, S and Wang, N and Oertle, A and Kozlikin, MB and Shunkov, MV and Derevianko, AP and Comeskey, D and Jope-Street, B and Harvey, VL and Chowdhury, MP and Buckley, M and Higham, T and Douka, K}, title = {Zooarchaeology through the lens of collagen fingerprinting at Denisova Cave.}, journal = {Scientific reports}, volume = {11}, number = {1}, pages = {15457}, pmid = {34326389}, issn = {2045-2322}, support = {18-09-40100//Russian Foundation for Basic Research/ ; UF120473//Royal Society/ ; 324139//Seventh Framework Programme/ ; 715069//H2020 European Research Council/ ; }, abstract = {Denisova Cave, a Pleistocene site in the Altai Mountains of Russian Siberia, has yielded significant fossil and lithic evidence for the Pleistocene in Northern Asia. Abundant animal and human bones have been discovered at the site, however, these tend to be highly fragmented, necessitating new approaches to identifying important hominin and faunal fossils. Here we report the results for 8253 bone fragments using ZooMS. Through the integration of this new ZooMS-based data with the previously published macroscopically-identified fauna we aim to create a holistic picture of the zooarchaeological record of the site. We identify trends associated with climate variability throughout the Middle and Upper Pleistocene as well as patterns explaining the process of bone fragmentation. Where morphological analysis of bones from the site have identified a high proportion of carnivore bones (30.2%), we find that these account for only 7.6% of the ZooMS assemblage, with large mammals between 3 and 5 more abundant overall. Our analysis suggests a cyclical pattern in fragmentation of bones which sees initial fragmentation by hominins using percussive tools and secondary carnivore action, such as gnawing and digestion, likely furthering the initial human-induced fragmentation.}, }
@article {pmid34320013, year = {2021}, author = {Condemi, S and Mazières, S and Faux, P and Costedoat, C and Ruiz-Linares, A and Bailly, P and Chiaroni, J}, title = {Blood groups of Neandertals and Denisova decrypted.}, journal = {PloS one}, volume = {16}, number = {7}, pages = {e0254175}, doi = {10.1371/journal.pone.0254175}, pmid = {34320013}, issn = {1932-6203}, abstract = {Blood group systems were the first phenotypic markers used in anthropology to decipher the origin of populations, their migratory movements, and their admixture. The recent emergence of new technologies based on the decoding of nucleic acids from an individual's entire genome has relegated them to their primary application, blood transfusion. Thus, despite the finer mapping of the modern human genome in relation to Neanderthal and Denisova populations, little is known about red cell blood groups in these archaic populations. Here we analyze the available high-quality sequences of three Neanderthals and one Denisovan individuals for 7 blood group systems that are used today in transfusion (ABO including H/Se, Rh (Rhesus), Kell, Duffy, Kidd, MNS, Diego). We show that Neanderthal and Denisova were polymorphic for ABO and shared blood group alleles recurrent in modern Sub-Saharan populations. Furthermore, we found ABO-related alleles currently preventing from viral gut infection and Neanderthal RHD and RHCE alleles nowadays associated with a high risk of hemolytic disease of the fetus and newborn. Such a common blood group pattern across time and space is coherent with a Neanderthal population of low genetic diversity exposed to low reproductive success and with their inevitable demise. Lastly, we connect a Neanderthal RHD allele to two present-day Aboriginal Australian and Papuan, suggesting that a segment of archaic genome was introgressed in this gene in non-Eurasian populations. While contributing to both the origin and late evolutionary history of Neanderthal and Denisova, our results further illustrate that blood group systems are a relevant piece of the puzzle helping to decipher it.}, }
@article {pmid34272242, year = {2021}, author = {Schaefer, NK and Shapiro, B and Green, RE}, title = {An ancestral recombination graph of human, Neanderthal, and Denisovan genomes.}, journal = {Science advances}, volume = {7}, number = {29}, pages = {}, doi = {10.1126/sciadv.abc0776}, pmid = {34272242}, issn = {2375-2548}, abstract = {Many humans carry genes from Neanderthals, a legacy of past admixture. Existing methods detect this archaic hominin ancestry within human genomes using patterns of linkage disequilibrium or direct comparison to Neanderthal genomes. Each of these methods is limited in sensitivity and scalability. We describe a new ancestral recombination graph inference algorithm that scales to large genome-wide datasets and demonstrate its accuracy on real and simulated data. We then generate a genome-wide ancestral recombination graph including human and archaic hominin genomes. From this, we generate a map within human genomes of archaic ancestry and of genomic regions not shared with archaic hominins either by admixture or incomplete lineage sorting. We find that only 1.5 to 7% of the modern human genome is uniquely human. We also find evidence of multiple bursts of adaptive changes specific to modern humans within the past 600,000 years involving genes related to brain development and function.}, }
@article {pmid34217618, year = {2021}, author = {Bezverbnaya, K and Moogk, D and Cummings, D and Baker, CL and Aarts, C and Denisova, G and Sun, M and McNicol, JD and Turner, RC and Rullo, AF and Foley, SR and Bramson, JL}, title = {Development of a B-cell maturation antigen-specific T-cell antigen coupler receptor for multiple myeloma.}, journal = {Cytotherapy}, volume = {}, number = {}, pages = {}, doi = {10.1016/j.jcyt.2021.05.007}, pmid = {34217618}, issn = {1477-2566}, abstract = {BACKGROUND AIMS: T cells engineered with synthetic receptors have delivered powerful therapeutic results for patients with relapsed/refractory hematologic malignancies. The authors have recently described the T-cell antigen coupler (TAC) receptor, which co-opts the endogenous T-cell receptor (TCR) and activates engineered T cells in an HLA-independent manner. Here the authors describe the evolution of a next-generation TAC receptor with a focus on developing a TAC-engineered T cell for multiple myeloma.<br><br>METHODS: To optimize the TAC scaffold, the authors employed a bona fide antigen-binding domain derived from the B-cell maturation antigen-specific monoclonal antibody C11D5.3, which has been used successfully in the clinic. The authors first tested humanized versions of the UCHT1 domain, which is used by the TAC to co-opt the TCR. The authors further discovered that the signal peptide affected surface expression of the TAC receptor. Higher density of the TAC receptor enhanced target binding in vitro, which translated into higher levels of Lck at the immunological synapse and stronger proliferation when only receptor-ligand interactions were present.<br><br>RESULTS: The authors observed that the humanized UCHT1 improved surface expression and in vivo efficacy. Using TAC T cells derived from both healthy donors and multiple myeloma patients, the authors determined that despite the influence of receptor density on early activation events and effector function, receptor density did not impact late effector functions in vitro, nor did the receptor density affect in vivo efficacy.<br><br>CONCLUSIONS: The modifications to the TAC scaffold described herein represent an important step in the evolution of this technology, which tolerates a range of expression levels without impacting therapeutic efficacy.}, }
@article {pmid34210857, year = {2021}, author = {Gibbons, A}, title = {'Dragon Man' may be an elusive Denisovan.}, journal = {Science (New York, N.Y.)}, volume = {373}, number = {6550}, pages = {11-12}, doi = {10.1126/science.373.6550.11}, pmid = {34210857}, issn = {1095-9203}, }
@article {pmid34201112, year = {2021}, author = {Sudarkina, OY and Filippenkov, IB and Stavchansky, VV and Denisova, AE and Yuzhakov, VV and Sevan'kaeva, LE and Valieva, LV and Remizova, JA and Dmitrieva, VG and Gubsky, LV and Myasoedov, NF and Limborska, SA and Dergunova, LV}, title = {Brain Protein Expression Profile Confirms the Protective Effect of the ACTH(4-7)PGP Peptide (Semax) in a Rat Model of Cerebral Ischemia-Reperfusion.}, journal = {International journal of molecular sciences}, volume = {22}, number = {12}, pages = {}, pmid = {34201112}, issn = {1422-0067}, support = {19-14-00268//Russian Science Foundation/ ; }, abstract = {The Semax (Met-Glu-His-Phe-Pro-Gly-Pro) peptide is a synthetic melanocortin derivative that is used in the treatment of ischemic stroke. Previously, studies of the molecular mechanisms underlying the actions of Semax using models of cerebral ischemia in rats showed that the peptide enhanced the transcription of neurotrophins and their receptors and modulated the expression of genes involved in the immune response. A genome-wide RNA-Seq analysis revealed that, in the rat transient middle cerebral artery occlusion (tMCAO) model, Semax suppressed the expression of inflammatory genes and activated the expression of neurotransmitter genes. Here, we aimed to evaluate the effect of Semax in this model via the brain expression profiling of key proteins involved in inflammation and cell death processes (MMP-9, c-Fos, and JNK), as well as neuroprotection and recovery (CREB) in stroke. At 24 h after tMCAO, we observed the upregulation of active CREB in subcortical structures, including the focus of the ischemic damage; downregulation of MMP-9 and c-Fos in the adjacent frontoparietal cortex; and downregulation of active JNK in both tissues under the action of Semax. Moreover, a regulatory network was constructed. In conclusion, the suppression of inflammatory and cell death processes and the activation of recovery may contribute to the neuroprotective action of Semax at both the transcriptome and protein levels.}, }
@article {pmid34183844, year = {2021}, author = {Graham, F}, title = {Daily briefing: DNA in Denisova Cave soil records several human species.}, journal = {Nature}, volume = {}, number = {}, pages = {}, doi = {10.1038/d41586-021-01771-9}, pmid = {34183844}, issn = {1476-4687}, }
@article {pmid34163072, year = {2021}, author = {Zavala, EI and Jacobs, Z and Vernot, B and Shunkov, MV and Kozlikin, MB and Derevianko, AP and Essel, E and de Fillipo, C and Nagel, S and Richter, J and Romagné, F and Schmidt, A and Li, B and O'Gorman, K and Slon, V and Kelso, J and Pääbo, S and Roberts, RG and Meyer, M}, title = {Pleistocene sediment DNA reveals hominin and faunal turnovers at Denisova Cave.}, journal = {Nature}, volume = {}, number = {}, pages = {}, pmid = {34163072}, issn = {1476-4687}, abstract = {Denisova Cave in southern Siberia is the type locality of the Denisovans, an archaic hominin group who were related to Neanderthals1-4. The dozen hominin remains recovered from the deposits also include Neanderthals5,6 and the child of a Neanderthal and a Denisovan7, which suggests that Denisova Cave was a contact zone between these archaic hominins. However, uncertainties persist about the order in which these groups appeared at the site, the timing and environmental context of hominin occupation, and the association of particular hominin groups with archaeological assemblages5,8-11. Here we report the analysis of DNA from 728 sediment samples that were collected in a grid-like manner from layers dating to the Pleistocene epoch. We retrieved ancient faunal and hominin mitochondrial (mt)DNA from 685 and 175 samples, respectively. The earliest evidence for hominin mtDNA is of Denisovans, and is associated with early Middle Palaeolithic stone tools that were deposited approximately 250,000 to 170,000 years ago; Neanderthal mtDNA first appears towards the end of this period. We detect a turnover in the mtDNA of Denisovans that coincides with changes in the composition of faunal mtDNA, and evidence that Denisovans and Neanderthals occupied the site repeatedly-possibly until, or after, the onset of the Initial Upper Palaeolithic at least 45,000 years ago, when modern human mtDNA is first recorded in the sediments.}, }
@article {pmid34150310, year = {2021}, author = {Amos, W}, title = {Correlated and geographically predictable Neanderthal and Denisovan legacies are difficult to reconcile with a simple model based on inter-breeding.}, journal = {Royal Society open science}, volume = {8}, number = {6}, pages = {201229}, pmid = {34150310}, issn = {2054-5703}, abstract = {Although the presence of archaic hominin legacies in humans is taken for granted, little attention has been given as to how the data fit with how humans colonized the world. Here, I show that Neanderthal and Denisovan legacies are strongly correlated and that inferred legacy size, like heterozygosity, exhibits a strong correlation with distance from Africa. Simulations confirm that, once created, legacy size is extremely stable: it may reduce through admixture with lower legacy populations but cannot increase significantly through neutral drift. Consequently, populations carrying the highest legacies are likely to be those whose ancestors inter-bred most with archaics. However, the populations with the highest legacies are globally scattered and are unified, not by having origins within the known Neanderthal range, but instead by living in locations that lie furthest from Africa. Furthermore, the Simons Genome Diversity Project data reveal two distinct correlations between Neanderthal and Denisovan legacies, one that starts in North Africa and increases west to east across Eurasia and into some parts of Oceania, and a second, much steeper trend that starts in Africa, peaking with the San and Ju/'hoansi and which, if extrapolated, predicts the large inferred legacies of both archaics found in Oceania/Australia. Similar 'double' trends are observed for the introgression statistic f 4 in a second large dataset published by Qin and Stoneking (Qin &amp; Stoneking 2015 Mol. Biol. Evol. 32, 2665-2674 (doi:10.1093/molbev/msv141)). These trends appear at odds with simple models of how introgression occurred though more complicated patterns of introgression could potentially generate better fits. Moreover, substituting archaic genomes with those of great apes yields similar but biologically impossible signals of introgression, suggesting that the signals these metrics capture arise within humans and are largely independent of the test group. Interestingly, the data do appear to fit a speculative model in which the loss of diversity that occurred when humans moved further from Africa created a gradient in heterozygosity that in turn progressively reduced mutation rate such that populations furthest from Africa have diverged less from our common ancestor and hence from the archaics. In this light, the two distinct trends could be interpreted in terms of two 'out of Africa' events, an early one ending in Oceania and Australia and a later one that colonized Eurasia and the Americas.}, }
@article {pmid34097675, year = {2021}, author = {Dergunova, LV and Dmitrieva, VG and Filippenkov, IB and Stavchansky, VV and Denisova, AE and Yuzhakov, VV and Sevan'kaeva, LE and Valieva, LV and Sudarkina, OY and Gubsky, LV and Myasoedov, NF and Limborska, SA}, title = {[The Peptide Drug ACTH(4-7)PGP (Semax) Suppresses mRNA Transcripts Encoding Proinflammatory Mediators Induced by Reversible Ischemia of the Rat Brain].}, journal = {Molekuliarnaia biologiia}, volume = {55}, number = {3}, pages = {402-411}, doi = {10.31857/S0026898421010043}, pmid = {34097675}, issn = {0026-8984}, mesh = {Adrenocorticotropic Hormone/analogs &amp; derivatives ; Animals ; Brain ; *Brain Ischemia/drug therapy/genetics ; Ischemia ; *Neuroprotective Agents ; Peptide Fragments ; *Pharmaceutical Preparations ; RNA, Messenger/genetics ; Rats ; Rats, Wistar ; }, abstract = {Due to its nootropic, neuroprotective, and immunomodulatory effects, the peptide Semax is utilized in the treatment of ischemic stroke. Our earlier RNA-Seq analysis of the transcriptome in an ischemic model of transient occlusion of the middle cerebral artery showed an increase in the mRNA levels of many proinflammatory genes, and the suppression of their induction by Semax. However, for many relevant genes, including Il1a, Il1b, Il6 and Tnfa, the levels of their expression were too low for detailed quantitative evaluation. Here we utilize qRT-PCR to analyze the effects of the Semax peptide on the expression of weakly expressed mRNAs encoding several proinflammatory mediators, and show that exposure to Semax leads to a statistically significant decrease in the Il1a, Il1b, Il6, Ccl3, and Cxcl2 mRNAs, which compensates for the increase in the transcription of these genes induced by ischemia-reperfusion. We conclude that the observed protective effect of Semax in the model of stroke may be due to its anti-inflammatory effects. We also discuss the limitations of the RNA-Seq when applied to quantifying less abundant transcripts as compared to the real-time RT-PCR method.}, }
@article {pmid34095864, year = {2021}, author = {Zhou, Y and Browning, SR}, title = {Protocol for detecting introgressed archaic variants with SPrime.}, journal = {STAR protocols}, volume = {2}, number = {2}, pages = {100550}, pmid = {34095864}, issn = {2666-1667}, abstract = {The SPrime program detects the variants in current-day populations that were introgressed from an archaic source in the past. It is optimized for detecting introgression from Neanderthals and Denisovans in modern humans. We provide a protocol for detecting Neanderthal and Denisovan introgression in 1000 Genomes Project data, specifically focusing on the CHB (Han Chinese in Beijing) population. For complete details on the use and execution of this protocol, please refer to Browning et al. (2018).}, }
@article {pmid34082629, year = {2021}, author = {Shtratnikova, V and Naumov, V and Bezuglov, V and Zheludkevich, A and Smigulina, L and Dikov, Y and Denisova, T and Suvorov, A and Pilsner, JR and Hauser, R and Krawetz, SA and Sergeyev, O}, title = {Optimization of small RNA extraction and comparative study of NGS library preparation from low count sperm samples.}, journal = {Systems biology in reproductive medicine}, volume = {67}, number = {3}, pages = {230-243}, doi = {10.1080/19396368.2021.1912851}, pmid = {34082629}, issn = {1939-6376}, abstract = {Recent studies demonstrate that sperm epigenome is a vehicle that conveys paternal experiences to offspring phenotype. That evidence triggers interest of both experimental and epidemiological studies of epigenetic markers in sperm. Since samples are often unique in epidemiological studies, a careful and efficient use of the material is a critical requirement. The goal of this study was to provide optimization of methods for the isolation of small RNAs from spermatozoa and library preparation for sequencing. A total 67 fractionated sperm samples from the Russian Children's Study biobank prospectively collected at 18-20 years of age were used to isolate small RNAs with median (IQR) input total sperm count 17.0 (7.4-35.9) million. Twenty-four pairs of libraries were prepared using the NEBNext and NEXTFlex kits, 19 libraries using NEBNext and 6 using NEXTFlex. All libraries were sequenced on NextSeq 500, and the results were evaluated as a function of the number of small non-coding RNA (sncRNA) detected, quality parameters of sequencing libraries, as well as technical features of sample preparation. Although the same amount of miRNA input was used for NEBNext and NEXTFlex libraries, the concentration of DNA in NEBNext libraries was significantly higher in comparison with NEXTFlex libraries. In high input (sperm count >28 million and more than 25 ng miRNA in library) NEXTFlex Small RNA-Seq kit detected more microRNAs. In low input, the NEBNext proved more effective. The tricks and traps to protocol optimization are presented, including an efficient and effector gel-based system for the removal of sequencing library adaptors.}, }
@article {pmid34072052, year = {2021}, author = {Denisova, YI and Shandryuk, GA and Arinina, MP and Levin, IS and Zhigarev, VA and Gringolts, ML and Finkelshtein, ES and Malkin, AY and Kudryavtsev, YV}, title = {Multiblock Copolymers of Norbornene and Cyclododecene: Chain Structure and Properties.}, journal = {Polymers}, volume = {13}, number = {11}, pages = {}, pmid = {34072052}, issn = {2073-4360}, support = {17-73-10450//Russian Science Foundation/ ; }, abstract = {We investigate the structure-property relations of the multiblock copolymers of norbornene with cyclododecene synthesized via the macromolecular cross-metathesis reaction between amorphous polynorbornene and semicrystalline polydodecenamer in the presence of the first-generation Grubbs catalyst. By adjusting the reaction time, catalyst amount, and composition of the initial system, we obtain a set of statistical multiblock copolymers that differ in the composition and average length of norbornene and dodecenylene unit sequences. Structural, thermal, and mechanical characterization of the copolymers with NMR, XRD, DSC (including thermal fractionation by successive self-nucleation and annealing), and rotational rheology allows us to relate the reaction conditions to the average length of crystallizable unit sequences, thicknesses of corresponding lamellas, and temperatures of their melting. We demonstrate that isolated dodecenylene units can be incorporated into crystalline lamellas so that even nearly random copolymers should retain crystallinity. Weak high-temperature endotherms observed in the multiblock copolymers of norbornene with cyclododecene and other cycloolefins could indicate that the corresponding systems are microphase-separated in the melt state.}, }
@article {pmid34050022, year = {2021}, author = {Zhang, X and Witt, KE and Bañuelos, MM and Ko, A and Yuan, K and Xu, S and Nielsen, R and Huerta-Sanchez, E}, title = {The history and evolution of the Denisovan-EPAS1 haplotype in Tibetans.}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {118}, number = {22}, pages = {}, pmid = {34050022}, issn = {1091-6490}, support = {R35 GM119856/GM/NIGMS NIH HHS/United States ; R35 GM128946/GM/NIGMS NIH HHS/United States ; T32 GM128596/GM/NIGMS NIH HHS/United States ; }, abstract = {Recent studies suggest that admixture with archaic hominins played an important role in facilitating biological adaptations to new environments. For example, interbreeding with Denisovans facilitated the adaptation to high-altitude environments on the Tibetan Plateau. Specifically, the EPAS1 gene, a transcription factor that regulates the response to hypoxia, exhibits strong signatures of both positive selection and introgression from Denisovans in Tibetan individuals. Interestingly, despite being geographically closer to the Denisova Cave, East Asian populations do not harbor as much Denisovan ancestry as populations from Melanesia. Recently, two studies have suggested two independent waves of Denisovan admixture into East Asians, one of which is shared with South Asians and Oceanians. Here, we leverage data from EPAS1 in 78 Tibetan individuals to interrogate which of these two introgression events introduced the EPAS1 beneficial sequence into the ancestral population of Tibetans, and we use the distribution of introgressed segment lengths at this locus to infer the timing of the introgression and selection event. We find that the introgression event unique to East Asians most likely introduced the beneficial haplotype into the ancestral population of Tibetans around 48,700 (16,000-59,500) y ago, and selection started around 9,000 (2,500-42,000) y ago. Our estimates suggest that one of the most convincing examples of adaptive introgression is in fact selection acting on standing archaic variation.}, }
@article {pmid34045664, year = {2021}, author = {Denisova, E and Westphal, D and Surowy, HM and Meier, F and Hutter, B and Reifenberger, J and Rütten, A and Schulz, A and Sergon, M and Ziemer, M and Brors, B and Betz, RC and Redler, S}, title = {Whole-exome sequencing in eccrine porocarcinoma indicates promising therapeutic strategies.}, journal = {Cancer gene therapy}, volume = {}, number = {}, pages = {}, pmid = {34045664}, issn = {1476-5500}, support = {2015.042.1//Wilhelm Sander-Stiftung (Wilhelm Sander Foundation)/ ; 2015.042.1//Wilhelm Sander-Stiftung (Wilhelm Sander Foundation)/ ; EXC2151//Deutsche Forschungsgemeinschaft (German Research Foundation)/ ; }, abstract = {Malignant sweat gland tumours are rare, with the most common form being Eccrine porocarcinoma (EP). To investigate the mutational landscape of EP, we performed whole-exome sequencing (WES) on 14 formalin-fixed paraffin-embedded samples of matched primary EP and healthy surrounding tissue. Mutational profiling revealed a high overall median mutation rate. This was attributed to signatures of mutational processes related to ultraviolet (UV) exposure, APOBEC enzyme dysregulation, and defective homologous double-strand break repair. All of these processes cause genomic instability and are implicated in carcinogenesis. Recurrent driving somatic alterations were detected in the EP candidate drivers TP53, FAT2, CACNA1S, and KMT2D. The analyses also identified copy number alterations and recurrent gains and losses in several chromosomal regions including that containing BRCA2, as well as deleterious alterations in multiple HRR components. In accordance with this reduced or even a complete loss of BRCA2 protein expression was detected in 50% of the investigated EP tumours. Our results implicate crucial oncogenic driver pathways and suggest that defective homologous double-strand break repair and the p53 pathway are involved in EP aetiology. Targeting of the p53 axis and PARP inhibition, and/or immunotherapy may represent promising treatment strategies.}, }
@article {pmid34028527, year = {2021}, author = {Ahlquist, K and Banuelos, M and Funk, A and Lai, J and Rong, S and Villanea, F and Witt, K}, title = {Our tangled family tree: new genomic methods offer insight into the legacy of archaic admixture.}, journal = {Genome biology and evolution}, volume = {}, number = {}, pages = {}, doi = {10.1093/gbe/evab115}, pmid = {34028527}, issn = {1759-6653}, abstract = {The archaic ancestry present in the human genome has captured the imagination of both scientists and the wider public in recent years. This excitement is the result of new studies pushing the envelope of what we can learn from the archaic genetic information that has survived for over 50,000 years in the human genome. Here, we review the most recent ten years of literature on the topic of archaic introgression, including the current state of knowledge on Neanderthal and Denisovan introgression, as well as introgression from other as-yet unidentified archaic populations. We focus this review on four topics: i) a reimagining of human demographic history, including evidence for multiple admixture events between modern humans, Neanderthals, Denisovans, and other archaic populations; ii) state-of-the-art methods for detecting archaic ancestry in population-level genomic data; iii) how these novel methods can detect archaic introgression in modern African populations; and iv) the functional consequences of archaic gene variants, including how those variants were co-opted into novel function in modern human populations. The goal of this review is to provide a simple-to-access reference for the relevant methods and novel data, which has changed our understanding of the relationship between our species and its siblings. This body of literature reveals the large degree to which the genetic legacy of these extinct hominins has been integrated into the human populations of today.}, }
@article {pmid33934123, year = {2021}, author = {Mualim, K and Theunert, C and Slatkin, M}, title = {Estimation of coalescence probabilities and population divergence times from SNP data.}, journal = {Heredity}, volume = {127}, number = {1}, pages = {1-9}, pmid = {33934123}, issn = {1365-2540}, abstract = {We present a method called the G(A|B) method for estimating coalescence probabilities within population lineages from genome sequences when one individual is sampled from each population. Population divergence times can be estimated from these coalescence probabilities if additional assumptions about the history of population sizes are made. Our method is based on a method presented by Rasmussen et al. (2014) to test whether an archaic genome is from a population directly ancestral to a present-day population. The G(A|B) method does not require distinguishing ancestral from derived alleles or assumptions about demographic history before population divergence. We discuss the relationship of our method to two similar methods, one introduced by Green et al. (2010) and called the F(A|B) method and the other introduced by Schlebusch et al. (2017) and called the TT method. When our method is applied to individuals from three or more populations, it provides a test of whether the population history is treelike because coalescence probabilities are additive on a tree. We illustrate the use of our method by applying it to three high-coverage archaic genomes, two Neanderthals (Vindija and Altai) and a Denisovan.}, }
@article {pmid33892510, year = {2021}, author = {Villanea, FA and Huerta-Sanchez, E and Fox, PK}, title = {ABO genetic variation in Neanderthals and Denisovans.}, journal = {Molecular biology and evolution}, volume = {}, number = {}, pages = {}, doi = {10.1093/molbev/msab109}, pmid = {33892510}, issn = {1537-1719}, abstract = {Variation at the ABO locus was one of the earliest sources of data in the study of human population identity and history, and to this day remains widely genotyped due to its importance in blood and tissue transfusions. Here, we look at ABO blood type variants in our archaic relatives: Neanderthals and Denisovans. Our goal is to understand the genetic landscape of the ABO gene in archaic humans, and how it relates to modern human ABO variation. We found two Neanderthal variants of the O allele in the Siberian Neanderthals (O1 and O2), one of these variants is shared with an European Neanderthal, who is a heterozygote for this O1 variant and a rare cis-AB variant. The Denisovan individual is heterozygous for two variants of the O1 allele, functionally similar to variants found widely in modern humans. Perhaps more surprisingly, the O2 allele variant found in Siberian Neanderthals can be found at low frequencies in modern Europeans and Southeast Asians, and the O1 allele variant found in Siberian and European Neanderthal is also found at very low frequency in modern East Asians. Our genetic distance analyses suggest both alleles survive in modern humans due to inbreeding with Neanderthals. We find that the sequence backgrounds of the surviving Neanderthal-like O alleles in modern humans retain a higher sequence divergence than other surviving Neanderthal genome fragments, supporting a view of balancing selection operating in the Neanderthal ABO alleles by retaining highly diverse haplotypes compared to portions of the genome evolving neutrally.}, }
@article {pmid33885362, year = {2021}, author = {Weiss, CV and Harshman, L and Inoue, F and Fraser, HB and Petrov, DA and Ahituv, N and Gokhman, D}, title = {The cis-regulatory effects of modern human-specific variants.}, journal = {eLife}, volume = {10}, number = {}, pages = {}, pmid = {33885362}, issn = {2050-084X}, support = {F31 HG011568/HG/NHGRI NIH HHS/United States ; 1R01MH109907/MH/NIMH NIH HHS/United States ; 1U01MH116438/MH/NIMH NIH HHS/United States ; 1UM1HG009408/HG/NHGRI NIH HHS/United States ; }, abstract = {The Neanderthal and Denisovan genomes enabled the discovery of sequences that differ between modern and archaic humans, the majority of which are noncoding. However, our understanding of the regulatory consequences of these differences remains limited, in part due to the decay of regulatory marks in ancient samples. Here, we used a massively parallel reporter assay in embryonic stem cells, neural progenitor cells, and bone osteoblasts to investigate the regulatory effects of the 14,042 single-nucleotide modern human-specific variants. Overall, 1791 (13%) of sequences containing these variants showed active regulatory activity, and 407 (23%) of these drove differential expression between human groups. Differentially active sequences were associated with divergent transcription factor binding motifs, and with genes enriched for vocal tract and brain anatomy and function. This work provides insight into the regulatory function of variants that emerged along the modern human lineage and the recent evolution of human gene expression.}, }
@article {pmid33883706, year = {2020}, author = {}, title = {Podcast: Denisovan DNA in modern Europeans, and the birth of an unusual celestial object.}, journal = {Nature}, volume = {}, number = {}, pages = {}, doi = {10.1038/d41586-020-01170-6}, pmid = {33883706}, issn = {1476-4687}, }
@article {pmid33877744, year = {2021}, author = {Denisova, K}, title = {Genetic vulnerability of exposures to antenatal maternal treatments in 1- to 2-month-old infants.}, journal = {Infancy : the official journal of the International Society on Infant Studies}, volume = {26}, number = {3}, pages = {515-532}, doi = {10.1111/infa.12398}, pmid = {33877744}, issn = {1532-7078}, support = {R01MH121605/MH/NIMH NIH HHS/United States ; 614242//Simons Foundation Autism Research Initiative/ ; //Sackler Award in Developmental Psychobiology/ ; }, abstract = {The growth and maturation of the nervous system are vulnerable during pregnancy. The impact of antenatal exposures to maternal treatments, in the context of genetic vulnerability of the fetus, on sensorimotor functioning in early infancy remains unexplored. Statistical features of head movements obtained from resting-state sleep fMRI scans are examined in 1- to 2-month-old infants, both those at high risk (HR) for autism spectrum disorder (ASD) due to a biological sibling with ASD and at low risk (LR) (N = 56). In utero exposures include maternal prescription medications (psychotropic Rx: N = 3HR ; N = 5LR vs. non-psychotropic Rx: N = 11HR ; N = 9LR vs. none: N = 11HR ; N = 16LR), psychiatric diagnoses (two or more Dx2 : N = 5HR ; N = 1LR ; one Dx1 : N = 4HR ; N = 5LR ; no Dx: N = 12HR ; N = 19LR), infections requiring antibiotics (infection: N = 5HR ; N = 8LR ; no infection: N = 20HR ; N = 22LR), or high fever (fever: N = 2HR ; N = 2LR ; no fever: N = 23HR ; N = 27LR). Movements with significantly higher variability are detected in infants exposed to psychotropics (e.g., opioid analgesics) and those whose mothers had fever, and this effect is significantly worse for infants at HR for ASD. Movements are significantly less variable in HR infants with non-psychotropic exposures (e.g., antibiotics). Heightened number of psychiatric or mental health conditions is associated with noisier movements in both risk groups. Genetic vulnerability due to in utero exposure to maternal treatments is an important future approach to be advanced in the field of early mind and brain development.}, }
@article {pmid33854233, year = {2021}, author = {Choin, J and Mendoza-Revilla, J and Arauna, LR and Cuadros-Espinoza, S and Cassar, O and Larena, M and Ko, AM and Harmant, C and Laurent, R and Verdu, P and Laval, G and Boland, A and Olaso, R and Deleuze, JF and Valentin, F and Ko, YC and Jakobsson, M and Gessain, A and Excoffier, L and Stoneking, M and Patin, E and Quintana-Murci, L}, title = {Genomic insights into population history and biological adaptation in Oceania.}, journal = {Nature}, volume = {592}, number = {7855}, pages = {583-589}, pmid = {33854233}, issn = {1476-4687}, abstract = {The Pacific region is of major importance for addressing questions regarding human dispersals, interactions with archaic hominins and natural selection processes1. However, the demographic and adaptive history of Oceanian populations remains largely uncharacterized. Here we report high-coverage genomes of 317 individuals from 20 populations from the Pacific region. We find that the ancestors of Papuan-related ('Near Oceanian') groups underwent a strong bottleneck before the settlement of the region, and separated around 20,000-40,000 years ago. We infer that the East Asian ancestors of Pacific populations may have diverged from Taiwanese Indigenous peoples before the Neolithic expansion, which is thought to have started from Taiwan around 5,000 years ago2-4. Additionally, this dispersal was not followed by an immediate, single admixture event with Near Oceanian populations, but involved recurrent episodes of genetic interactions. Our analyses reveal marked differences in the proportion and nature of Denisovan heritage among Pacific groups, suggesting that independent interbreeding with highly structured archaic populations occurred. Furthermore, whereas introgression of Neanderthal genetic information facilitated the adaptation of modern humans related to multiple phenotypes (for example, metabolism, pigmentation and neuronal development), Denisovan introgression was primarily beneficial for immune-related functions. Finally, we report evidence of selective sweeps and polygenic adaptation associated with pathogen exposure and lipid metabolism in the Pacific region, increasing our understanding of the mechanisms of biological adaptation to island environments.}, }
@article {pmid33825739, year = {2021}, author = {Gulaĭ, IS and Snegirev, AI and Denisova, NP and Dmitriev, AB}, title = {[Chronic spinal stimulation in treatment of lower limb critical ischaemia syndrome].}, journal = {Angiologiia i sosudistaia khirurgiia = Angiology and vascular surgery}, volume = {27}, number = {1}, pages = {128-135}, doi = {10.33529/ANGIO2020413}, pmid = {33825739}, issn = {1027-6661}, mesh = {*Arterial Occlusive Diseases ; Arteries ; Humans ; Ischemia/diagnosis/etiology/therapy ; Leg ; Lower Extremity ; *Peripheral Arterial Disease ; }, abstract = {Obliterating peripheral artery disease is a commonly occurring pathological condition, most often resulting from an atherosclerotic lesion of vessels with progressive narrowing of their lumens. The consequences of decompensation of chronic arterial insufficiency such as ischaemic pain, claudication, and trophic impairments are in some instances difficult to treat, despite using multicomponent medicamentous therapy and/or performing revascularizing interventions. This article describes a clinical case report regarding the use of spinal stimulation in a patient presenting with stage IV chronic lower limb ischaemia according to the Fontaine classification. This is accompanied and followed by depicting the dynamics of the laboratory, instrumental, and clinical parameters over a two-year follow-up period. In order to explain the choice of the intervention and the causes of the described picture, discussed are the existing theories of the mechanisms of action of spinal stimulation. To this is added a literature review of using this method in treatment of lower limb critical ischaemia when performing reconstructive angiosurgical treatment is unavailable. Mention is also made of the incidence and types of probable complications, as well as possibilities and limitations of the method.}, }
@article {pmid33759196, year = {2021}, author = {Shunatova, N and Denisova, S and Shchenkov, S}, title = {Ultrastructure of rhizoids in the marine bryozoan Dendrobeania fruticosa (Gymnolaemata: Cheilostomata).}, journal = {Journal of morphology}, volume = {282}, number = {6}, pages = {847-862}, doi = {10.1002/jmor.21351}, pmid = {33759196}, issn = {1097-4687}, support = {20-04-01031//Russian Foundation for Basic Research/ ; }, abstract = {Bryozoans form colonies of iterated modules, termed zooids, and display varying degrees of polymorphism. Polymorphic colonies comprise autozooids (or feeding zooids) and heteromorphic zooids, among which the most common types are avicularia and kenozooids. Kenozooids differ in shape, size, and presumed function. Among this diversity, there are rhizoids, which serve to attach colonies to the substrate or to lift them above it. To date, only general data on anatomy of kenozooids at light microscopy level are available. Here, we present the first description of the ultrastructure of the holdfast-like rhizoids of the cheilostome bryozoan Dendrobeania fruticosa. The rhizoid wall is composed of a single-layered epidermis, which produces the ectocyst. The voluminous cavity is acoelomate: it has no special cellular lining, nor any signs of an extracellular matrix toward the epidermis. It is traversed by delicate branching funicular strands that originate from the pore plate. The only cells in contact with the epidermis are the cells of the funicular system and the storage cells. The pore plate between the rhizoid and autozooid includes a variable number of communication pores. Each pore is plugged with a rosette complex, which includes a cincture cell and four special cells extending through the pore. The limiting cells are absent, and the special cells are in direct contact with the funicular strands. Cell contacts between special cells are absent; moreover, there are spaces between their proximal lobes filled with a heterogeneous matrix similar to that in the lumen of the funicular strands. Such matrix is also found outside of the extracellular matrix surrounding the special cells. These findings allow us to suggest that nutrient transport most likely occurs between, rather than through, the special cells. However, further studies are needed to understand how the rosette complex functions.}, }
@article {pmid33753899, year = {2021}, author = {Teixeira, JC and Jacobs, GS and Stringer, C and Tuke, J and Hudjashov, G and Purnomo, GA and Sudoyo, H and Cox, MP and Tobler, R and Turney, CSM and Cooper, A and Helgen, KM}, title = {Widespread Denisovan ancestry in Island Southeast Asia but no evidence of substantial super-archaic hominin admixture.}, journal = {Nature ecology &amp; evolution}, volume = {5}, number = {5}, pages = {616-624}, pmid = {33753899}, issn = {2397-334X}, mesh = {Animals ; Asia, Southeastern ; Fossils ; *Hominidae/genetics ; Humans ; Islands ; *Neanderthals ; }, abstract = {The hominin fossil record of Island Southeast Asia (ISEA) indicates that at least two endemic 'super-archaic' species-Homo luzonensis and H. floresiensis-were present around the time anatomically modern humans arrived in the region >50,000 years ago. Intriguingly, contemporary human populations across ISEA carry distinct genomic traces of ancient interbreeding events with Denisovans-a separate hominin lineage that currently lacks a fossil record in ISEA. To query this apparent disparity between fossil and genetic evidence, we performed a comprehensive search for super-archaic introgression in >400 modern human genomes, including >200 from ISEA. Our results corroborate widespread Denisovan ancestry in ISEA populations, but fail to detect any substantial super-archaic admixture signals compatible with the endemic fossil record of ISEA. We discuss the implications of our findings for the understanding of hominin history in ISEA, including future research directions that might help to unlock more details about the prehistory of the enigmatic Denisovans.}, }
@article {pmid33743527, year = {2021}, author = {Denisova, K and Barmettler, A}, title = {Evaluating the Thyroid Eye Disease Patient.}, journal = {International ophthalmology clinics}, volume = {61}, number = {2}, pages = {33-52}, doi = {10.1097/IIO.0000000000000351}, pmid = {33743527}, issn = {1536-9617}, mesh = {*Graves Ophthalmopathy/diagnosis/therapy ; Humans ; }, }
@article {pmid33720649, year = {2021}, author = {Soldatsky, YL and Denisova, OA and Vitkovskaya, IP and Krugovskaya, NL}, title = {[Modern causes of tracheostomy in children].}, journal = {Vestnik otorinolaringologii}, volume = {86}, number = {1}, pages = {36-40}, doi = {10.17116/otorino20218601136}, pmid = {33720649}, issn = {0042-4668}, mesh = {*Airway Obstruction ; Child ; Humans ; Infant, Newborn ; Intubation, Intratracheal ; Respiration, Artificial ; Retrospective Studies ; *Tracheostomy/adverse effects ; }, abstract = {The purpose of work is to analyze the causes of tracheostomy in children hospitalized in a large multidisciplinary pediatric hospital.<br><br>MATERIAL AND METHODS: Retrospective analysis of case of children treated in a multidisciplinary urgent hospital - GBUZ «Morozovskaya CCCH of MDH», which in the period from 01.01.16 to 31.12.18 was made operation «tracheostomy» was conducted.<br><br>RESULTS: Tracheostomy was performed in 138 (0.064%) among 216 469 hospitalized children. Age at the time of tracheostomy ranged from 2 weeks to 17.5 years (on average 67.9±59.84 months, Me=47.5 months), and 36.2% of children had tracheostomy was done on the 1st year of life. 126 (91.3%) patients required prolonged tracheal intubation prior to tracheostomy placement; the duration of intubation ranged from 1 to 95 days (on average 19.9±13.42 days, Me=14 days). The main reasons of tracheostomy were the need for long-term mechanical ventilation/respiratory support; the need for constant sanitation of the lower respiratory tract with bulbar/pseudobulbar disorders; upper respiratory paths obstruction. The diseases that led to this condition can be grouped into 4 categories: CNS pathology - 76 (55.1%) patients; brain / spinal cord tumors - 36 (26.1%); neurodystrophy and stenosis of the upper respiratory tract of various etiology - 13 (9.4% each) patients. 68.1% of patients were found incurable and required palliative care. Mortality among patients with a known catamnesis was 39.1%, mainly due to progression of the underlying disease; the lethality associated with tracheal cannulation was 1.4%.<br><br>CONCLUSION: Currently, pediatric tracheostomy is moving into the category of predominantly planned surgical interventions. More than 2/3 of children requiring tracheostomy are patients in need of palliative care with severe pathology of the central nervous system; in which the main indications for surgery are the need for respiration support and regular tracheobronchial care..}, }
@article {pmid33659800, year = {2020}, author = {Makarova, EN and Yakovleva, TV and Balyibina, NY and Baranov, KO and Denisova, EI and Dubinina, AD and Feofanova, NA and Bazhan, NM}, title = {Pharmacological effects of fibroblast growth factor 21 are sex-specific in mice with the lethal yellow (Ay) mutation.}, journal = {Vavilovskii zhurnal genetiki i selektsii}, volume = {24}, number = {2}, pages = {200-208}, doi = {10.18699/VJ20.40-o}, pmid = {33659800}, issn = {2500-0462}, abstract = {Hypothalamic melanocortin 4 receptors (MC4R) regulate energy balance. Mutations in the MC4R gene are the most common cause of monogenic obesity in humans. Fibroblast growth factor 21 (FGF21) is a promising antiobesity agent, but its effects on melanocortin obesity are unknown. Sex is an important biological variable that must be considered when conducting preclinical studies; however, in laboratory animal models, the pharmacological effects of FGF21 are well documented only for male mice. We aimed at investigating whether FGF21 affects metabolism in male and female mice with the lethal yellow (Ay) mutation, which results in MC4R blockage and obesity development. Obese C57Bl-Ay male and female mice were administered subcutaneously for 10 days with vehicle or FGF21 (1 mg per 1 kg). Food intake (FI), body weight (BW), blood parameters, and gene expression in the liver, muscles, brown adipose tissue, subcutaneous and visceral white adipose tissues, and hypothalamus were measured. FGF21 action strongly depended on the sex of the animals. In the males, FGF21 decreased BW and insulin blood levels without affecting FI. In the females, FGF21 increased FI and liver weight, but did not affect BW. In control Ay-mice, expression of genes involved in lipid and glucose metabolism (Ppargc1a, Cpt1, Pck1, G6p, Slc2a2) in the liver and genes involved in lipogenesis (Pparg, Lpl, Slc2a4) in visceral adipose tissue was higher in females than in males, and FGF21 administration inhibited the expression of these genes in females. FGF21 administration decreased hypothalamic POMC mRNA only in males. Thus, the pharmacological effect of FGF21 were significantly different in male and female Ay-mice; unlike males, females were resistant to catabolic effects of FGF21.}, }
@article {pmid33580755, year = {2021}, author = {Gusev, EI and Blokhin, VE and Vartanov, SA and Martynov, MY and Katunina, EA and Alesenko, AV and Denisova, IA and Pavlova, EN and Polterovich, VM and Kucheryanu, VG and Shupik, MA and Nodel, MR and Kalinkin, AL and Sokolov, SA and Chubarova, TV and Shakleina, MV and Pronina, TS and Ugryumov, MV}, title = {[Development of early diagnosis of Parkinson's disease and comprehensive economic analysis of the effect of its implementation].}, journal = {Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova}, volume = {121}, number = {1}, pages = {9-20}, doi = {10.17116/jnevro20211210119}, pmid = {33580755}, issn = {1997-7298}, mesh = {Animals ; Biomarkers ; Early Diagnosis ; Humans ; *Parkinson Disease/diagnosis ; Positron-Emission Tomography ; Prodromal Symptoms ; }, abstract = {The paper summarizes the literature and author's data on the development of early (preclinical) diagnosis of Parkinson's disease (PD). Implementation of this diagnosis will promote the use of preventive therapy and change investments in diagnosis and treatment of patients. The paper declares that at present the only approach to early diagnosis of PD is positron-emission tomography of the nigrostriatal dopaminergic system, but it cannot be used for preventive examination due to its high cost. The authors consider that a less specific, but more promising approach to the development of early diagnosis of PD is the search for markers in body fluids, mainly in the blood, in patients at the prodromal stage of PD. Indeed, a number of markers as changes in the level of metabolites of monoamines, sphingolipids, urates, and indicators of oxidative stress were found in patients selected for the risk group of the prodromal stage of PD, according to characteristic premotor symptoms. In addition, it is assumed that the search for blood markers at an earlier - pre-prodromal stage is possible only in animal models of PD at the early preclinical stage. This approach can also be used to verify blood markers identified in patients at the clinical stage of PD. It is also evident that the complex socio-economic factors influencing the incidence of PD is different in developed versus developing countries. The societal and medical costs of Parkinson's are huge and efforts to improve early preclinical diagnosis of PD will lead to considerable economical and societal benefits. For instance this will allow efficient selection of patients for preclinical diagnostic tests. To assess the effectiveness of this strategy considering the uncertainty of socio-economic issues, a modification of the «cost-utility» analysis is proposed. For the first time, a Markov model of PD including preclinical diagnostic tests and possible neuroprotective therapy was developed and studied. Analytical outcomes of this process suggest that the idea of developing a new multimodal strategy is promising from a socio-economic point of view.}, }
@article {pmid33574182, year = {2021}, author = {Trujillo, CA and Rice, ES and Schaefer, NK and Chaim, IA and Wheeler, EC and Madrigal, AA and Buchanan, J and Preissl, S and Wang, A and Negraes, PD and Szeto, RA and Herai, RH and Huseynov, A and Ferraz, MSA and Borges, FS and Kihara, AH and Byrne, A and Marin, M and Vollmers, C and Brooks, AN and Lautz, JD and Semendeferi, K and Shapiro, B and Yeo, GW and Smith, SEP and Green, RE and Muotri, AR}, title = {Reintroduction of the archaic variant of NOVA1 in cortical organoids alters neurodevelopment.}, journal = {Science (New York, N.Y.)}, volume = {371}, number = {6530}, pages = {}, pmid = {33574182}, issn = {1095-9203}, support = {R01 HL137223/HL/NHLBI NIH HHS/United States ; K12 GM068524/GM/NIGMS NIH HHS/United States ; R01 MH121487/MH/NIMH NIH HHS/United States ; U41 HG009889/HG/NHGRI NIH HHS/United States ; S10 OD026929/OD/NIH HHS/United States ; R01 HG004659/HG/NHGRI NIH HHS/United States ; R01 MH113545/MH/NIMH NIH HHS/United States ; K01 AA026911/AA/NIAAA NIH HHS/United States ; U19 MH107367/MH/NIMH NIH HHS/United States ; }, mesh = {Alleles ; Alternative Splicing ; Amino Acid Substitution ; Animals ; Binding Sites ; Biological Evolution ; CRISPR-Cas Systems ; Cell Proliferation ; Cerebral Cortex/cytology/*growth &amp; development/*physiology ; Gene Expression Regulation, Developmental ; Genetic Variation ; Genome ; Genome, Human ; Haplotypes ; Hominidae/genetics ; Humans ; Induced Pluripotent Stem Cells ; Neanderthals/*genetics ; Nerve Net/physiology ; Nerve Tissue Proteins/genetics/metabolism ; Neurons/*physiology ; Organoids ; RNA-Binding Proteins/*genetics/*metabolism ; Synapses/physiology ; }, abstract = {The evolutionarily conserved splicing regulator neuro-oncological ventral antigen 1 (NOVA1) plays a key role in neural development and function. NOVA1 also includes a protein-coding difference between the modern human genome and Neanderthal and Denisovan genomes. To investigate the functional importance of an amino acid change in humans, we reintroduced the archaic allele into human induced pluripotent cells using genome editing and then followed their neural development through cortical organoids. This modification promoted slower development and higher surface complexity in cortical organoids with the archaic version of NOVA1 Moreover, levels of synaptic markers and synaptic protein coassociations correlated with altered electrophysiological properties in organoids expressing the archaic variant. Our results suggest that the human-specific substitution in NOVA1, which is exclusive to modern humans since divergence from Neanderthals, may have had functional consequences for our species' evolution.}, }
@article {pmid33556445, year = {2021}, author = {Reinscheid, RK and Mafessoni, F and Lüttjohann, A and Jüngling, K and Pape, HC and Schulz, S}, title = {Neandertal introgression and accumulation of hypomorphic mutations in the neuropeptide S (NPS) system promote attenuated functionality.}, journal = {Peptides}, volume = {138}, number = {}, pages = {170506}, doi = {10.1016/j.peptides.2021.170506}, pmid = {33556445}, issn = {1873-5169}, abstract = {The neuropeptide S (NPS) system plays an important role in fear and fear memory processing but has also been associated with allergic and inflammatory diseases. Genes for NPS and its receptor NPSR1 are found in all tetrapods. Compared to non-human primates, several non-synonymous single-nucleotide polymorphisms (SNPs) occur in both human genes that collectively result in functional attenuation, suggesting adaptive mechanisms in a human context. To investigate historic and geographic origins of these hypomorphic mutations and explore genetic signs of selection, we analyzed ancient genomes and worldwide genotype frequencies of four prototypic SNPs in the NPS system. Neandertal and Denisovan genomes contain exclusively ancestral alleles for NPSR1 while all derived alleles occur in ancient genomes of anatomically modern humans, indicating that they arose in modern Homo sapiens. Worldwide genotype frequencies for three hypomorphic NPSR1 SNPs show significant regional homogeneity but follow a gradient towards increasing derived allele frequencies that supports an out-of-Africa scenario. Increased density of high-frequency polymorphisms around the three NPSR1 loci suggests weak or possibly balancing selection. A hypomorphic mutation in the NPS precursor, however, was detected at high frequency in Eurasian Neandertal genomes and shows genetic signatures indicating that it was introgressed into the human gene pool, particularly in Southern Europe, by interbreeding with Neandertals. We discuss potential evolutionary scenarios including behavior and immune-based natural selection.}, }
@article {pmid33547071, year = {2021}, author = {Bonfante, B and Faux, P and Navarro, N and Mendoza-Revilla, J and Dubied, M and Montillot, C and Wentworth, E and Poloni, L and Varón-González, C and Jones, P and Xiong, Z and Fuentes-Guajardo, M and Palmal, S and Chacón-Duque, JC and Hurtado, M and Villegas, V and Granja, V and Jaramillo, C and Arias, W and Barquera, R and Everardo-Martínez, P and Sánchez-Quinto, M and Gómez-Valdés, J and Villamil-Ramírez, H and Silva de Cerqueira, CC and Hünemeier, T and Ramallo, V and Liu, F and Weinberg, SM and Shaffer, JR and Stergiakouli, E and Howe, LJ and Hysi, PG and Spector, TD and Gonzalez-José, R and Schüler-Faccini, L and Bortolini, MC and Acuña-Alonzo, V and Canizales-Quinteros, S and Gallo, C and Poletti, G and Bedoya, G and Rothhammer, F and Thauvin-Robinet, C and Faivre, L and Costedoat, C and Balding, D and Cox, T and Kayser, M and Duplomb, L and Yalcin, B and Cotney, J and Adhikari, K and Ruiz-Linares, A}, title = {A GWAS in Latin Americans identifies novel face shape loci, implicating VPS13B and a Denisovan introgressed region in facial variation.}, journal = {Science advances}, volume = {7}, number = {6}, pages = {}, pmid = {33547071}, issn = {2375-2548}, support = {R01 DE027023/DE/NIDCR NIH HHS/United States ; U01 DE020078/DE/NIDCR NIH HHS/United States ; R00 DE024194/DE/NIDCR NIH HHS/United States ; R01 DE016148/DE/NIDCR NIH HHS/United States ; R01 DE028945/DE/NIDCR NIH HHS/United States ; R35 GM119465/GM/NIGMS NIH HHS/United States ; }, abstract = {To characterize the genetic basis of facial features in Latin Americans, we performed a genome-wide association study (GWAS) of more than 6000 individuals using 59 landmark-based measurements from two-dimensional profile photographs and ~9,000,000 genotyped or imputed single-nucleotide polymorphisms. We detected significant association of 32 traits with at least 1 (and up to 6) of 32 different genomic regions, more than doubling the number of robustly associated face morphology loci reported until now (from 11 to 23). These GWAS hits are strongly enriched in regulatory sequences active specifically during craniofacial development. The associated region in 1p12 includes a tract of archaic adaptive introgression, with a Denisovan haplotype common in Native Americans affecting particularly lip thickness. Among the nine previously unidentified face morphology loci we identified is the VPS13B gene region, and we show that variants in this region also affect midfacial morphology in mice.}, }
@article {pmid33459549, year = {2020}, author = {Kondratieva, EA and Kondratev, SA and Denisova, AA and Ivanova, NE and Kondratiev, AN}, title = {[Results of treatment with intravenous amantadine sulfate (PK-Merz) patients with chronic disorders of consciousness].}, journal = {Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova}, volume = {120}, number = {12}, pages = {102-108}, doi = {10.17116/jnevro2020120121102}, pmid = {33459549}, issn = {1997-7298}, mesh = {*Amantadine ; *Consciousness ; Consciousness Disorders ; Humans ; Persistent Vegetative State ; Wakefulness ; }, abstract = {The article presents literature review of the recent publications devoted to the drugs with dopaminergic, antiglutamatergic and GABA-ergic effects in the treatment of patients with vegetative state/areactive wakefulness syndrome (VS/AWS). The authors analyzed their own results of the effects of intravenous form of amantadine sulfate (PK Merz) in 142 VS/AWS patients caused by different etiological factors. Depending on the dominant neurological symptoms, patients were divided into three main groups: areactive type of course (group 1 - 61 patients), predominance of primitive limbic reactions (group 2 - 35 patients) and predominance of extrapyramidal symptoms (group 3 - 46 patients). Therapy results were evaluated one month later by CRS-R scale, which showed that the most distinct positive dynamics was observed in group 3.}, }
@article {pmid33417716, year = {2021}, author = {Greer, C and Bhakta, H and Ghanem, L and Refai, F and Linn, E and Avella, M}, title = {Deleterious variants in genes regulating mammalian reproduction in Neanderthals, Denisovans and extant humans.}, journal = {Human reproduction (Oxford, England)}, volume = {36}, number = {3}, pages = {734-755}, doi = {10.1093/humrep/deaa347}, pmid = {33417716}, issn = {1460-2350}, mesh = {Animals ; Europe ; Genome, Human ; *Hominidae/genetics ; Humans ; Male ; *Neanderthals/genetics ; Nuclear Proteins ; Reproduction/genetics ; Trans-Activators ; }, abstract = {STUDY QUESTION: Were Neanderthals and Denisovans (referred here also as extinct hominidae) carrying deleterious variants in genes regulating reproduction?<br><br>SUMMARY ANSWER: The majority of extinct hominidae analyzed here, presented a considerable number of deleterious variants per individual in proteins regulating different aspects of reproduction, including gonad and uterine function, and gametogenesis.<br><br>WHAT IS KNOWN ALREADY: Neanderthals, Denisovans and extant humans were interfertile and hybridized while occupying geographically overlapping areas in Europe and Asia. This is evidenced by the small archaic genome component (average ∼2%) present in non-African extant humans.<br><br>STUDY DESIGN, SIZE, DURATION: The genome of eight extinct hominidae, together with five human genome databases, plus 44 mothers and 48 fathers (fertile controls), were screened to look for deleterious variants in 1734 protein-coding genes regulating reproduction.<br><br>Ancient DNA from six Neanderthals and two Denisovans dated between ∼82 000 and 43 000 calibrated years was retrieved from the public European Nucleotide Archive. The hominins analyzed include Altai, Vindija 33.15, 33.19, 33.25 and 33.26, El Sidron 1253, Denisova 3 and 11. Their DNA was analyzed using the CLC Genomics Workbench 12, by mapping overlapping paired-end reads (Illumina, FASTQ files) to the human genome assembly GRCh37 (hg19) (Vindija 33.19, 33.25, 33.26, Denisova 3 and Denisova 11) or by analyzing BAM files (Altai, El Sidron 1253 and Vindija 33.15) (human genome reference, GRCh37 (hg19)). Non-synonymous reproductive variants were classified as deleterious or tolerated (PolyPhen-2 and SIFT analyses) and were compared to deleterious variants obtained from extant human genome databases (Genome Aggregation Database (GnomAD), 1000 Genomes, the Haplotype Map (HapMap), Single Nucleotide Polymorphism Database (dbSNPs)) across different populations. A genetic intersection between extant or extinct DNA variants and other genetic disorders was evaluated by annotating the obtained variants with the Clinical Variant (ClinVar) database.<br><br>Among the eight extinct hominidae analyzed, a total of 9650 non-synonymous variants (only coverage ≥20 reads included; frameshift mutations were excluded) in 1734 reproductive protein-coding genes were found, 24% of which were classified as deleterious. The majority (73%) of the deleterious alleles present in extant humans that are shared between extant humans and extinct hominidae were found to be rare (<1%) in extant human populations. A set of 8044 variants were found uniquely in extinct hominidae. At the single-gene level, no extinct individual was found to be homozygous for deleterious variants in genes necessary for gamete recognition and fusion, and no higher chance of embryo-lethality (calculated by Mendelian Genetics) was found upon simulated mating between extant human and extinct hominidae compared to extant human-extant human. However, three of the eight extinct hominidae were found to be homozygous for 48-69 deleterious variants in 55 genes controlling ovarian and uterine functions, or oogenesis (AKAP1, BUB1B, CCDC141, CDC73, DUSP6, ESR1, ESR2, PATL2, PSMC3IP, SEMA3A, WT1 and WNT4). Moreover, we report the distribution of nine Neanderthal variants in genes associated with a human fertility phenotype found in extant human populations, one of which has been associated with polycystic ovarian syndrome and primary congenital glaucoma.<br><br>While analyzing archaic DNA, stringent filtering criteria were adopted to screen for deleterious variants in Neanderthals and Denisovans, which could result in missing a number of variants. Such restraints preserve the potential for detection of additional deleterious variants in reproductive proteins in extinct hominidae.<br><br>This study provides a comprehensive overview of putatively deleterious variants in extant human populations and extinct individuals occurring in 1734 protein-coding genes controlling reproduction and provides the fundaments for future functional studies of extinct variants in human reproduction.<br><br>This study was supported by the Department of Biological Science and by the Office of Research and Sponsored Programs at the University of Tulsa (Faculty Research Grant and Faculty Research Summer Fellowship) to M.A. and the University of Tulsa, Tulsa Undergraduate Research Challenge (TURC) program to E.L.; no conflict of interest to declare.<br><br>TRIAL REGISTRATION NUMBER: N/A.}, }
@article {pmid33407590, year = {2021}, author = {Alexeeva, E and Horneff, G and Dvoryakovskaya, T and Denisova, R and Nikishina, I and Zholobova, E and Malievskiy, V and Santalova, G and Stadler, E and Balykova, L and Spivakovskiy, Y and Kriulin, I and Alshevskaya, A and Moskalev, A}, title = {Early combination therapy with etanercept and methotrexate in JIA patients shortens the time to reach an inactive disease state and remission: results of a double-blind placebo-controlled trial.}, journal = {Pediatric rheumatology online journal}, volume = {19}, number = {1}, pages = {5}, pmid = {33407590}, issn = {1546-0096}, abstract = {BACKGROUND: Remission is the primary objective of treating juvenile idiopathic arthritis (JIA). It is still debatable whether early intensive treatment is superior in terms of earlier achievement of remission. The aim of this study was to evaluate the effectiveness of early etanercept+methotrexate (ETA+MTX) combination therapy versus step-up MTX monotherapy with ETA added in refractory disease.<br><br>METHODS: A multi-centre, double-blind, randomized study in active polyarticular JIA patients treated with either ETA+MTX (n = 35) or placebo+MTX (n = 33) for up to 24 weeks, followed by a 24-week open-label phase. The efficacy endpoints included pedACR30 criteria improvement at week 12, inactive disease at week 24, and remission at week 48. Patients who failed to achieve the endpoints at week 12 or at week 24 escaped to open-label ETA+MTX. Safety was assessed at each visit.<br><br>RESULTS: By intention-to-treat analysis, more patients in the ETA+MTX group reached the pedACR30 response at week 12 (33 (94.3%)) than in the placebo+MTX group (20 (60.6%); p = 0.001). At week 24, comparable percentages of patients reached inactive disease (11 (31.4%) vs 11 (33.3%)). At week 48, 11 (31.4%) and eight (24.2%) patients achieved remission. The median (+/-IQR) times to achieve an inactive disease state in the ETA+MTX and placebo+MTX groups were 24 (14-32) and 32 (24-40) weeks, respectively. Forty-four (74/100 patient-years) adverse events (AEs) were reported, leading to treatment discontinuation in 6 patients.<br><br>CONCLUSIONS: Early combination therapy with ETA+MTX proved to be highly effective compared to the standard step-up regimen. Compared to those treated with the standard regimen, more patients treated with a combination of ETA+MTX reached the pedACR30 response and achieved inactive disease and remission more rapidly.}, }
@article {pmid33215809, year = {2021}, author = {Bentzon, AK and Panteleev, A and Mitsura, V and Borodulina, E and Skrahina, A and Denisova, E and Tetradov, S and Podlasin, R and Riekstina, V and Kancauskiene, Z and Paduto, D and Mocroft, A and Trofimova, T and Miller, R and Post, F and Grezesczuk, A and Lundgren, JD and Inglot, M and Podlekareva, D and Bolokadze, N and Kirk, O and , }, title = {Healthcare delivery for HIV-positive people with tuberculosis in Europe.}, journal = {HIV medicine}, volume = {22}, number = {4}, pages = {283-293}, doi = {10.1111/hiv.13016}, pmid = {33215809}, issn = {1468-1293}, support = {RP-2016-07-012/DH_/Department of Health/United Kingdom ; }, abstract = {BACKGROUND: In a 2013 survey, we reported distinct discrepancies in delivery of tuberculosis (TB) and HIV services in eastern Europe (EE) vs. western Europe (WE).<br><br>OBJECTIVES: To verify the differences in TB and HIV services in EE vs. WE.<br><br>METHODS: Twenty-three sites completed a survey in 2018 (EE, 14; WE, nine; 88% response rate). Results were compared across as well as within the two regions. When possible, results were compared with the 2013 survey.<br><br>RESULTS: Delivery of healthcare was significantly less integrated in EE: provision of TB and HIV services at one site (36% in EE vs. 89% in WE; P = 0.034), and continued TB follow-up in one location (42% vs. 100%; P = 0.007). Although access to TB diagnostics, standard TB and HIV drugs was generally good, fewer sites in EE reported unlimited access to rifabutin/multi-drug-resistant TB (MDR-TB) drugs, HIV integrase inhibitors and opioid substitution therapy (OST). Compared with 2013, routine usage of GeneXpert was more common in EE in 2018 (54% vs. 92%; P = 0.073), as was access to moxifloxacin (46% vs. 91%; P = 0.033), linezolid (31% vs. 64%; P = 0.217), and bedaquiline (0% vs. 25%; P = 0.217). Integration of TB and HIV services (46% vs. 39%; P = 1.000) and provision of OST to patients with opioid dependency (54% vs. 46%; P = 0.695) remained unchanged.<br><br>CONCLUSION: Delivery of TB and HIV healthcare, including integration of TB and HIV care and access to MDR-TB drugs, still differs between WE and EE, as well as between individual EE sites.}, }
@article {pmid33183249, year = {2020}, author = {Cook, S and Kudryavtsev, AV and Bobrova, N and Saburova, L and Denisova, D and Malyutina, S and Lewis, G and Leon, DA}, title = {Prevalence of symptoms, ever having received a diagnosis and treatment of depression and anxiety, and associations with health service use amongst the general population in two Russian cities.}, journal = {BMC psychiatry}, volume = {20}, number = {1}, pages = {537}, pmid = {33183249}, issn = {1471-244X}, support = {100217/WT_/Wellcome Trust/United Kingdom ; N/A//UiT The Arctic University of Norway/ ; N/A//Norwegian Institute of Public Health/ ; N/A//Norwegian Ministry of Health and Social Affairs/ ; }, mesh = {Adult ; Aged ; *Anxiety/diagnosis/epidemiology/therapy ; Cities ; Cross-Sectional Studies ; *Depression/diagnosis/drug therapy/epidemiology ; Female ; Health Services ; Humans ; Male ; Middle Aged ; Prevalence ; Russia/epidemiology ; }, abstract = {BACKGROUND: Little is known about the burden of common mental disorders in Russia despite high levels of suicide and alcohol-related mortality. Here we investigated levels of symptoms, self-reports of ever having received a diagnosis and treatment of anxiety and depression in two Russian cities.<br><br>METHODS: The study population was men and women aged 35-69 years old participating in cross-sectional population-based studies in the cities of Arkhangelsk and Novosibirsk (2015-18). Participants completed an interview which included the PHQ-9 and GAD-7 scales, questions on whether participants had ever received a diagnosis of depression or anxiety, and health service use in the past year. Participants also reported current medication use and medications were coded in line with the WHO anatomical therapeutic classification (ATC). Depression was defined as PHQ-9 ≥ 10 and Anxiety as GAD-7 ≥ 10.<br><br>RESULTS: Age-standardised prevalence of PHQ-9 ≥ 10 was 10.7% in women and 5.4% in men (GAD-7 ≥ 10 6.2% in women; 3.0% in men). Among those with PHQ-9 ≥ 10 17% reported ever having been diagnosed with depression (equivalent finding for anxiety 29%). Only 1.5% of those with PHQ-9 ≥ 10 reported using anti-depressants and 0.6% of those with GAD-7 ≥ 10 reported using anxiolytics. No men with PHQ-9 ≥ 10 and/or GAD-7 ≥ 10 reported use of anti-depressants or anxiolytics. Use of health services increased with increasing severity of both depression and anxiety.<br><br>CONCLUSION: There was a large gap between symptoms and reporting of past diagnosis and treatment of common mental disorders in two Russian cities. Interventions aimed at improving mental health literacy and reducing stigma could be of benefit in closing this substantial treatment gap.}, }
@article {pmid33183137, year = {2020}, author = {Aston, S and Denisova, K and Hurlbert, A and Olkkonen, M and Pearce, B and Rudd, M and Werner, A and Xiao, B}, title = {Exploring the Determinants of Color Perception Using #Thedress and Its Variants: The Role of Spatio-Chromatic Context, Chromatic Illumination, and Material-Light Interaction.}, journal = {Perception}, volume = {49}, number = {11}, pages = {1235-1251}, pmid = {33183137}, issn = {1468-4233}, support = {/WT_/Wellcome Trust/United Kingdom ; P20 GM103650/GM/NIGMS NIH HHS/United States ; R01 MH121605/MH/NIMH NIH HHS/United States ; }, abstract = {The colors that people see depend not only on the surface properties of objects but also on how these properties interact with light as well as on how light reflected from objects interacts with an individual's visual system. Because individual visual systems vary, the same visual stimulus may elicit different perceptions from different individuals. #thedress phenomenon drove home this point: different individuals viewed the same image and reported it to be widely different colors: blue and black versus white and gold. This phenomenon inspired a collection of demonstrations presented at the Vision Sciences Society 2015 Meeting which showed how spatial and temporal manipulations of light spectra affect people's perceptions of material colors and illustrated the variability in individual color perception. The demonstrations also explored the effects of temporal alterations in metameric lights, including Maxwell's Spot, an entoptic phenomenon. Crucially, the demonstrations established that #thedress phenomenon occurs not only for images of the dress but also for the real dress under real light sources of different spectral composition and spatial configurations.}, }
@article {pmid33180850, year = {2020}, author = {Vorobieva, NV and Makunin, AI and Druzhkova, AS and Kusliy, MA and Trifonov, VA and Popova, KO and Polosmak, NV and Molodin, VI and Vasiliev, SK and Shunkov, MV and Graphodatsky, AS}, title = {High genetic diversity of ancient horses from the Ukok Plateau.}, journal = {PloS one}, volume = {15}, number = {11}, pages = {e0241997}, pmid = {33180850}, issn = {1932-6203}, mesh = {Animals ; Animals, Domestic/*genetics ; Animals, Wild/*genetics ; DNA, Ancient/analysis ; Evolution, Molecular ; Extinction, Biological ; Fossils/history ; Genome, Mitochondrial ; Haplotypes ; High-Throughput Nucleotide Sequencing/veterinary ; History, Ancient ; Horses ; Mitochondria/*genetics ; Phylogeny ; Russia ; Whole Genome Sequencing/*veterinary ; }, abstract = {A growing number of researchers studying horse domestication come to a conclusion that this process happened in multiple locations and involved multiple wild maternal lines. The most promising approach to address this problem involves mitochondrial haplotype comparison of wild and domestic horses from various locations coupled with studies of possible migration routes of the ancient shepherds. Here, we sequenced complete mitochondrial genomes of six horses from burials of the Ukok plateau (Russia, Altai Mountains) dated from 2.7 to 1.4 thousand years before present and a single late Pleistocene wild horse from the neighboring region (Denisova cave). Sequencing data indicates that the wild horse belongs to an extinct pre-domestication lineage. Integration of the domestic horse data with known Eurasian haplotypes of a similar age revealed two distinct groups: the first one widely distributed in Europe and presumably imported to Altai, and the second one specific for Altai Mountains and surrounding area.}, }
@article {pmid33168070, year = {2020}, author = {Nesterenko, MA and Starunov, VV and Shchenkov, SV and Maslova, AR and Denisova, SA and Granovich, AI and Dobrovolskij, AA and Khalturin, KV}, title = {Molecular signatures of the rediae, cercariae and adult stages in the complex life cycles of parasitic flatworms (Digenea: Psilostomatidae).}, journal = {Parasites &amp; vectors}, volume = {13}, number = {1}, pages = {559}, pmid = {33168070}, issn = {1756-3305}, support = {МК-2105.2017.4//Council on grants of the President of the Russian Federation/ ; 19-34-90111//RFBR/ ; }, abstract = {BACKGROUND: Parasitic flatworms (Trematoda: Digenea) represent one of the most remarkable examples of drastic morphological diversity among the stages within a life cycle. Which genes are responsible for extreme differences in anatomy, physiology, behavior, and ecology among the stages? Here we report a comparative transcriptomic analysis of parthenogenetic and amphimictic generations in two evolutionary informative species of Digenea belonging to the family Psilostomatidae.<br><br>METHODS: In this study the transcriptomes of rediae, cercariae and adult worm stages of Psilotrema simillimum and Sphaeridiotrema pseudoglobulus, were sequenced and analyzed. High-quality transcriptomes were generated, and the reference sets of protein-coding genes were used for differential expression analysis in order to identify stage-specific genes. Comparative analysis of gene sets, their expression dynamics and Gene Ontology enrichment analysis were performed for three life stages within each species and between the two species.<br><br>RESULTS: Reference transcriptomes for P. simillimum and S. pseudoglobulus include 21,433 and 46,424 sequences, respectively. Among 14,051 orthologous groups (OGs), 1354 are common and specific for two analyzed psilostomatid species, whereas 13 and 43 OGs were unique for P. simillimum and S. pseudoglobulus, respectively. In contrast to P. simillimum, where more than 60% of analyzed genes were active in the redia, cercaria and adult worm stages, in S. pseudoglobulus less than 40% of genes had such a ubiquitous expression pattern. In general, 7805 (36.41%) and 30,622 (65.96%) of genes were preferentially expressed in one of the analyzed stages of P. simillimum and S. pseudoglobulus, respectively. In both species 12 clusters of co-expressed genes were identified, and more than a half of the genes belonging to the reference sets were included into these clusters. Functional specialization of the life cycle stages was clearly supported by Gene Ontology enrichment analysis.<br><br>CONCLUSIONS: During the life cycles of the two species studied, most of the genes change their expression levels considerably, consequently the molecular signature of a stage is not only a unique set of expressed genes, but also the specific levels of their expression. Our results indicate unexpectedly high level of plasticity in gene regulation between closely related species. Transcriptomes of P. simillimum and S. pseudoglobulus provide high quality reference resource for future evolutionary studies and comparative analyses.}, }
@article {pmid33149716, year = {2020}, author = {Mahesh, S and Denisova, T and Gerasimova, L and Pakhmutova, N and Mallappa, M and Vithoulkas, G}, title = {Multimorbidity After Surgical Menopause Treated with Individualized Classical Homeopathy: A Case Report.}, journal = {Clinical medicine insights. Case reports}, volume = {13}, number = {}, pages = {1179547620965560}, pmid = {33149716}, issn = {1179-5476}, abstract = {Classical homeopathy was shown to be beneficial in climacteric syndrome in many studies, but the clinical effect is unclear. To inspect if individualized classical homeopathy has a role in treating complaints after surgical menopause through real world case, we present a case of a 54-year-old Russian woman treated with individualized classical homeopathy for multimorbid conditions after surgical menopause examined for changes from homeopathic treatment. We assessed changes in climacteric symptoms, changes in comorbidities, and the general well-being of the patient. The woman had severe climacteric syndrome, pelvic inflammatory disease, dyslipidemia, obesity, hepatic steatosis, pancreatic lipomatosis, gall bladder disease, and mild subclinical hypothyroidism to begin with. She was treated with individualized classical homeopathy and followed up for 31 months. She was relieved of the vasomotor symptoms and psychological disturbances of climacteric syndrome, her weight reduced, the ultrasound scan showed absence of lipomatosis/gall bladder disease/hepatic steatosis. Blood tests showed reduction of thyroid stimulating hormone and a balance in the lipid status. Individualized classical homeopathy may have a role in the climacteric syndrome and comorbidities after surgical menopause. The efficacy of homeopathic therapy in climacteric problems must be scientifically investigated further.}, }
@article {pmid33133486, year = {2020}, author = {Chernyshev, VM and Denisova, EA and Eremin, DB and Ananikov, VP}, title = {The key role of R-NHC coupling (R = C, H, heteroatom) and M-NHC bond cleavage in the evolution of M/NHC complexes and formation of catalytically active species.}, journal = {Chemical science}, volume = {11}, number = {27}, pages = {6957-6977}, pmid = {33133486}, issn = {2041-6520}, abstract = {Complexes of metals with N-heterocyclic carbene ligands (M/NHC) are typically considered the systems of choice in homogeneous catalysis due to their stable metal-ligand framework. However, it becomes obvious that even metal species with a strong M-NHC bond can undergo evolution in catalytic systems, and processes of M-NHC bond cleavage are common for different metals and NHC ligands. This review is focused on the main types of the M-NHC bond cleavage reactions and their impact on activity and stability of M/NHC catalytic systems. For the first time, we consider these processes in terms of NHC-connected and NHC-disconnected active species derived from M/NHC precatalysts and classify them as fundamentally different types of catalysts. Problems of rational catalyst design and sustainability issues are discussed in the context of the two different types of M/NHC catalysis mechanisms.}, }
@article {pmid33122381, year = {2020}, author = {Zhang, D and Xia, H and Chen, F and Li, B and Slon, V and Cheng, T and Yang, R and Jacobs, Z and Dai, Q and Massilani, D and Shen, X and Wang, J and Feng, X and Cao, P and Yang, MA and Yao, J and Yang, J and Madsen, DB and Han, Y and Ping, W and Liu, F and Perreault, C and Chen, X and Meyer, M and Kelso, J and Pääbo, S and Fu, Q}, title = {Denisovan DNA in Late Pleistocene sediments from Baishiya Karst Cave on the Tibetan Plateau.}, journal = {Science (New York, N.Y.)}, volume = {370}, number = {6516}, pages = {584-587}, doi = {10.1126/science.abb6320}, pmid = {33122381}, issn = {1095-9203}, mesh = {Animals ; *Caves ; DNA, Ancient/*isolation &amp; purification ; DNA, Mitochondrial/genetics ; Geologic Sediments/*chemistry ; Hominidae/*classification/*genetics ; Humans ; Phylogeny ; Tibet ; }, abstract = {A late Middle Pleistocene mandible from Baishiya Karst Cave (BKC) on the Tibetan Plateau has been inferred to be from a Denisovan, an Asian hominin related to Neanderthals, on the basis of an amino acid substitution in its collagen. Here we describe the stratigraphy, chronology, and mitochondrial DNA extracted from the sediments in BKC. We recover Denisovan mitochondrial DNA from sediments deposited ~100 thousand and ~60 thousand years ago (ka) and possibly as recently as ~45 ka. The long-term occupation of BKC by Denisovans suggests that they may have adapted to life at high altitudes and may have contributed such adaptations to modern humans on the Tibetan Plateau.}, }
@article {pmid33122380, year = {2020}, author = {Massilani, D and Skov, L and Hajdinjak, M and Gunchinsuren, B and Tseveendorj, D and Yi, S and Lee, J and Nagel, S and Nickel, B and Devièse, T and Higham, T and Meyer, M and Kelso, J and Peter, BM and Pääbo, S}, title = {Denisovan ancestry and population history of early East Asians.}, journal = {Science (New York, N.Y.)}, volume = {370}, number = {6516}, pages = {579-583}, doi = {10.1126/science.abc1166}, pmid = {33122380}, issn = {1095-9203}, mesh = {Animals ; Asian Continental Ancestry Group/*genetics ; DNA, Ancient ; *Evolution, Molecular ; Female ; Hominidae/*genetics ; Humans ; Mongolia ; Population ; Skull ; }, abstract = {We present analyses of the genome of a ~34,000-year-old hominin skull cap discovered in the Salkhit Valley in northeastern Mongolia. We show that this individual was a female member of a modern human population that, following the split between East and West Eurasians, experienced substantial gene flow from West Eurasians. Both she and a 40,000-year-old individual from Tianyuan outside Beijing carried genomic segments of Denisovan ancestry. These segments derive from the same Denisovan admixture event(s) that contributed to present-day mainland Asians but are distinct from the Denisovan DNA segments in present-day Papuans and Aboriginal Australians.}, }
@article {pmid33122362, year = {2020}, author = {Gibbons, A}, title = {Denisovan DNA found in cave on Tibetan Plateau.}, journal = {Science (New York, N.Y.)}, volume = {370}, number = {6516}, pages = {512-513}, doi = {10.1126/science.370.6516.512}, pmid = {33122362}, issn = {1095-9203}, mesh = {Altitude ; Animals ; Caves ; Cold Temperature ; DNA, Ancient/*isolation &amp; purification ; DNA, Mitochondrial/*isolation &amp; purification ; Geologic Sediments/*chemistry ; Hominidae/*genetics ; Humans ; Tibet ; }, }
@article {pmid33039881, year = {2020}, author = {Pan, L and Dumoncel, J and Mazurier, A and Zanolli, C}, title = {Hominin diversity in East Asia during the Middle Pleistocene: A premolar endostructural perspective.}, journal = {Journal of human evolution}, volume = {148}, number = {}, pages = {102888}, doi = {10.1016/j.jhevol.2020.102888}, pmid = {33039881}, issn = {1095-8606}, abstract = {Following the recent studies of East Asian mid-Middle to early Late Pleistocene hominin material, a large spectrum of morphological diversity has been recognized and the coexistence of archaic ('Homo erectus-like') and derived ('modern-like') dental morphological patterns has been highlighted. In fact, for most of these Chinese fossils, generally categorized as 'archaic Homo sapiens' or 'post-H. erectus Homo', the taxonomic attribution is a matter of contention. With the help of μCT techniques and a deformation-based 3D geometric morphometric approach, we focused on the morphological variation in the enamel-dentine junction (EDJ) of 18 upper and lower premolars from Chinese Middle Pleistocene hominins. We then compared our results with a number of fossil and modern human groups, including Early Pleistocene H. erectus from Sangiran; late Early Pleistocene hominins from Tighenif, Algeria; classic Neanderthals; and modern humans. Our results highlight an evolutionary/chronological trend of crown base reduction, elevation of EDJ topography, and EDJ surface simplification in the hominin groups studied here. Moreover, this study brings insights to the taxonomy/phylogeny of 6 late Middle Pleistocene specimens whose evolutionary placement has been debated for decades. Among these specimens, Changyang premolars show features that can be aligned with the Asian H. erectus hypodigm, whereas Panxian Dadong and Tongzi premolars are more similar to Late Pleistocene Homo. Compared with early to mid-Middle Pleistocene hominins in East Asia, late Middle Pleistocene hominins evince an enlarged morphological variation. A persistence of archaic morphotypes and possible admixture among populations during the late Middle Pleistocene are discussed.}, }
@article {pmid32973032, year = {2020}, author = {Petr, M and Hajdinjak, M and Fu, Q and Essel, E and Rougier, H and Crevecoeur, I and Semal, P and Golovanova, LV and Doronichev, VB and Lalueza-Fox, C and de la Rasilla, M and Rosas, A and Shunkov, MV and Kozlikin, MB and Derevianko, AP and Vernot, B and Meyer, M and Kelso, J}, title = {The evolutionary history of Neanderthal and Denisovan Y chromosomes.}, journal = {Science (New York, N.Y.)}, volume = {369}, number = {6511}, pages = {1653-1656}, doi = {10.1126/science.abb6460}, pmid = {32973032}, issn = {1095-9203}, support = {/ERC_/European Research Council/International ; }, mesh = {Animals ; Chromosomes, Human, Y/genetics ; DNA, Ancient ; DNA, Mitochondrial/genetics ; *Evolution, Molecular ; Humans ; *Life History Traits ; Male ; Neanderthals/classification/*genetics ; Phylogeny ; Y Chromosome/*genetics ; }, abstract = {Ancient DNA has provided new insights into many aspects of human history. However, we lack comprehensive studies of the Y chromosomes of Denisovans and Neanderthals because the majority of specimens that have been sequenced to sufficient coverage are female. Sequencing Y chromosomes from two Denisovans and three Neanderthals shows that the Y chromosomes of Denisovans split around 700 thousand years ago from a lineage shared by Neanderthals and modern human Y chromosomes, which diverged from each other around 370 thousand years ago. The phylogenetic relationships of archaic and modern human Y chromosomes differ from the population relationships inferred from the autosomal genomes and mirror mitochondrial DNA phylogenies, indicating replacement of both the mitochondrial and Y chromosomal gene pools in late Neanderthals. This replacement is plausible if the low effective population size of Neanderthals resulted in an increased genetic load in Neanderthals relative to modern humans.}, }
@article {pmid32954276, year = {2020}, author = {Merisaari, J and Denisova, OV and Doroszko, M and Le Joncour, V and Johansson, P and Leenders, WPJ and Kastrinsky, DB and Zaware, N and Narla, G and Laakkonen, P and Nelander, S and Ohlmeyer, M and Westermarck, J}, title = {Monotherapy efficacy of blood-brain barrier permeable small molecule reactivators of protein phosphatase 2A in glioblastoma.}, journal = {Brain communications}, volume = {2}, number = {1}, pages = {fcaa002}, pmid = {32954276}, issn = {2632-1297}, abstract = {Glioblastoma is a fatal disease in which most targeted therapies have clinically failed. However, pharmacological reactivation of tumour suppressors has not been thoroughly studied as yet as a glioblastoma therapeutic strategy. Tumour suppressor protein phosphatase 2A is inhibited by non-genetic mechanisms in glioblastoma, and thus, it would be potentially amendable for therapeutic reactivation. Here, we demonstrate that small molecule activators of protein phosphatase 2A, NZ-8-061 and DBK-1154, effectively cross the in vitro model of blood-brain barrier, and in vivo partition to mouse brain tissue after oral dosing. In vitro, small molecule activators of protein phosphatase 2A exhibit robust cell-killing activity against five established glioblastoma cell lines, and nine patient-derived primary glioma cell lines. Collectively, these cell lines have heterogeneous genetic background, kinase inhibitor resistance profile and stemness properties; and they represent different clinical glioblastoma subtypes. Moreover, small molecule activators of protein phosphatase 2A were found to be superior to a range of kinase inhibitors in their capacity to kill patient-derived primary glioma cells. Oral dosing of either of the small molecule activators of protein phosphatase 2A significantly reduced growth of infiltrative intracranial glioblastoma tumours. DBK-1154, with both higher degree of brain/blood distribution, and more potent in vitro activity against all tested glioblastoma cell lines, also significantly increased survival of mice bearing orthotopic glioblastoma xenografts. In summary, this report presents a proof-of-principle data for blood-brain barrier-permeable tumour suppressor reactivation therapy for glioblastoma cells of heterogenous molecular background. These results also provide the first indications that protein phosphatase 2A reactivation might be able to challenge the current paradigm in glioblastoma therapies which has been strongly focused on targeting specific genetically altered cancer drivers with highly specific inhibitors. Based on demonstrated role for protein phosphatase 2A inhibition in glioblastoma cell drug resistance, small molecule activators of protein phosphatase 2A may prove to be beneficial in future glioblastoma combination therapies.}, }
@article {pmid32881095, year = {2020}, author = {Eremin, DB and Boiko, DA and Kostyukovich, AY and Burykina, JV and Denisova, EA and Anania, M and Martens, J and Berden, G and Oomens, J and Roithová, J and Ananikov, VP}, title = {Mechanistic Study of Pd/NHC-Catalyzed Sonogashira Reaction: Discovery of NHC-Ethynyl Coupling Process.}, journal = {Chemistry (Weinheim an der Bergstrasse, Germany)}, volume = {26}, number = {67}, pages = {15672-15681}, doi = {10.1002/chem.202003533}, pmid = {32881095}, issn = {1521-3765}, support = {17-13-01526//Russian Science Foundation/ ; }, abstract = {The product of a revealed transformation-NHC-ethynyl coupling-was observed as a catalyst transformation pathway in the Sonogashira cross-coupling, catalyzed by Pd/NHC complexes. The 2-ethynylated azolium salt was isolated in individual form and fully characterized, including X-ray analysis. A number of possible intermediates of this transformation with common formulae (NHC)n Pd(C2 Ph) (n=1,2) were observed and subjected to collision-induced dissociation (CID) and infrared multiphoton dissociation (IRMPD) experiments to elucidate their structure. Measured bond dissociation energies (BDEs) and IRMPD spectra were in an excellent agreement with quantum calculations for coupling product π-complexes with Pd0 . Molecular dynamics simulations confirmed the observed multiple CID fragmentation pathways. An unconventional methodology to study catalyst evolution suggests the reported transformation to be considered in the development of new catalytic systems for alkyne functionalization reactions.}, }
@article {pmid32839541, year = {2020}, author = {Telis, N and Aguilar, R and Harris, K}, title = {Selection against archaic hominin genetic variation in regulatory regions.}, journal = {Nature ecology &amp; evolution}, volume = {4}, number = {11}, pages = {1558-1566}, pmid = {32839541}, issn = {2397-334X}, support = {R35 GM133428/GM/NIGMS NIH HHS/United States ; }, mesh = {Animals ; Biological Evolution ; *Hominidae/genetics ; Humans ; *Neanderthals/genetics ; Regulatory Sequences, Nucleic Acid ; Selection, Genetic ; }, abstract = {Traces of Neandertal and Denisovan DNA persist in the modern human gene pool, but have been systematically purged by natural selection from genes and other functionally important regions. This implies that many archaic alleles harmed the fitness of hybrid individuals, but the nature of this harm is poorly understood. Here, we show that enhancers contain less Neandertal and Denisovan variation than expected given the background selection they experience, suggesting that selection acted to purge these regions of archaic alleles that disrupted their gene regulatory functions. We infer that selection acted mainly on young archaic variation that arose in Neandertals or Denisovans shortly before their contact with humans; enhancers are not depleted of older variants found in both archaic species. Some types of enhancer appear to have tolerated introgression better than others; compared with tissue-specific enhancers, pleiotropic enhancers show stronger depletion of archaic single-nucleotide polymorphisms. To some extent, evolutionary constraint is predictive of introgression depletion, but certain tissues' enhancers are more depleted of Neandertal and Denisovan alleles than expected given their comparative tolerance to new mutations. Foetal brain and muscle are the tissues whose enhancers show the strongest depletion of archaic alleles, but only brain enhancers show evidence of unusually stringent purifying selection. We conclude that epistatic incompatibilities between human and archaic alleles are needed to explain the degree of archaic variant depletion from foetal muscle enhancers, perhaps due to divergent selection for higher muscle mass in archaic hominins compared with humans.}, }
@article {pmid32783351, year = {2021}, author = {Quartier, P and Alexeeva, E and Constantin, T and Chasnyk, V and Wulffraat, N and Palmblad, K and Wouters, C and I Brunner, H and Marzan, K and Schneider, R and Horneff, G and Martini, A and Anton, J and Wei, X and Slade, A and Ruperto, N and Abrams, K and , }, title = {Tapering Canakinumab Monotherapy in Patients With Systemic Juvenile Idiopathic Arthritis in Clinical Remission: Results From a Phase IIIb/IV Open-Label, Randomized Study.}, journal = {Arthritis &amp; rheumatology (Hoboken, N.J.)}, volume = {73}, number = {2}, pages = {336-346}, pmid = {32783351}, issn = {2326-5205}, mesh = {Adolescent ; Antibodies, Monoclonal, Humanized/*administration &amp; dosage ; Antirheumatic Agents/*administration &amp; dosage/therapeutic use ; Arthritis, Juvenile/*drug therapy/physiopathology ; Child ; Child, Preschool ; Deprescriptions ; Drug Tapering/*methods ; Female ; Glucocorticoids/therapeutic use ; Humans ; Male ; Methotrexate/therapeutic use ; Remission Induction ; }, abstract = {OBJECTIVE: To evaluate the efficacy and safety of 2 canakinumab monotherapy tapering regimens in order to maintain complete clinical remission in children with systemic juvenile idiopathic arthritis (JIA).<br><br>METHODS: The study was designed as a 2-part phase IIIb/IV open-label, randomized trial. In the first part, patients received 4 mg/kg of canakinumab subcutaneously every 4 weeks and discontinued glucocorticoids and/or methotrexate as appropriate. Patients in whom clinical remission was achieved (inactive disease for at least 24 weeks) with canakinumab monotherapy were entered into the second part of the trial, in which they were randomized 1:1 into 1 of 2 treatment arms. In arm 1, the dose of canakinumab was reduced from 4 mg/kg to 2 mg/kg and then to 1 mg/kg, followed by discontinuation. In arm 2, the 4 mg/kg dose interval was prolonged from every 4 weeks, to every 8 weeks, and then to every 12 weeks, followed by discontinuation. In both arms, canakinumab exposure could be reduced provided systemic JIA remained in clinical remission for 24 weeks with each step. The primary objective was to assess whether >40% of randomized patients in either arm maintained clinical remission of systemic JIA for 24 weeks in the first part of the study.<br><br>RESULTS: In part 1 of the study, 182 patients were enrolled, with 75 of those patients randomized before entering part 2 of the trial. Among the 75 randomized patients, clinical remission was maintained for 24 weeks in 27 (71%) of 38 patients in arm 1 (2 mg/kg every 4 weeks) and 31 (84%) of 37 patients in arm 2 (4 mg/kg every 8 weeks) (P ≤ 0.0001 for arm 1 versus arm 2 among those meeting the 40% threshold). Overall, 25 (33%) of 75 patients discontinued canakinumab, and clinical remission was maintained for at least 24 weeks in all 25 of these patients. No new safety signals were identified.<br><br>CONCLUSION: Reduction of canakinumab exposure may be feasible in patients who have achieved clinical remission of systemic JIA, but consistent interleukin-1 inhibition appears necessary to maintain this response.}, }
@article {pmid32760067, year = {2020}, author = {Hubisz, MJ and Williams, AL and Siepel, A}, title = {Mapping gene flow between ancient hominins through demography-aware inference of the ancestral recombination graph.}, journal = {PLoS genetics}, volume = {16}, number = {8}, pages = {e1008895}, pmid = {32760067}, issn = {1553-7404}, support = {R35 GM127070/GM/NIGMS NIH HHS/United States ; }, mesh = {Animals ; Evolution, Molecular ; *Gene Flow ; Human Migration ; Humans ; *Models, Genetic ; Neanderthals/*genetics ; Population/*genetics ; *Recombination, Genetic ; }, abstract = {The sequencing of Neanderthal and Denisovan genomes has yielded many new insights about interbreeding events between extinct hominins and the ancestors of modern humans. While much attention has been paid to the relatively recent gene flow from Neanderthals and Denisovans into modern humans, other instances of introgression leave more subtle genomic evidence and have received less attention. Here, we present a major extension of the ARGweaver algorithm, called ARGweaver-D, which can infer local genetic relationships under a user-defined demographic model that includes population splits and migration events. This Bayesian algorithm probabilistically samples ancestral recombination graphs (ARGs) that specify not only tree topologies and branch lengths along the genome, but also indicate migrant lineages. The sampled ARGs can therefore be parsed to produce probabilities of introgression along the genome. We show that this method is well powered to detect the archaic migration into modern humans, even with only a few samples. We then show that the method can also detect introgressed regions stemming from older migration events, or from unsampled populations. We apply it to human, Neanderthal, and Denisovan genomes, looking for signatures of older proposed migration events, including ancient humans into Neanderthal, and unknown archaic hominins into Denisovans. We identify 3% of the Neanderthal genome that is putatively introgressed from ancient humans, and estimate that the gene flow occurred between 200-300kya. We find no convincing evidence that negative selection acted against these regions. Finally, we predict that 1% of the Denisovan genome was introgressed from an unsequenced, but highly diverged, archaic hominin ancestor. About 15% of these "super-archaic" regions-comprising at least about 4Mb-were, in turn, introgressed into modern humans and continue to exist in the genomes of people alive today.}, }
@article {pmid32750315, year = {2020}, author = {Course, MM and Gudsnuk, K and Smukowski, SN and Winston, K and Desai, N and Ross, JP and Sulovari, A and Bourassa, CV and Spiegelman, D and Couthouis, J and Yu, CE and Tsuang, DW and Jayadev, S and Kay, MA and Gitler, AD and Dupre, N and Eichler, EE and Dion, PA and Rouleau, GA and Valdmanis, PN}, title = {Evolution of a Human-Specific Tandem Repeat Associated with ALS.}, journal = {American journal of human genetics}, volume = {107}, number = {3}, pages = {445-460}, pmid = {32750315}, issn = {1537-6605}, support = {T32 GM007454/GM/NIGMS NIH HHS/United States ; U24 AG041689/AG/NIA NIH HHS/United States ; R01 DK078424/DK/NIDDK NIH HHS/United States ; P30 AG066509/AG/NIA NIH HHS/United States ; P50 AG005136/AG/NIA NIH HHS/United States ; R01 HG010169/HG/NHGRI NIH HHS/United States ; }, mesh = {Adaptor Proteins, Signal Transducing/*genetics ; Aged ; Alzheimer Disease/genetics/pathology ; Amyotrophic Lateral Sclerosis/*genetics/pathology ; DNA Repeat Expansion/genetics ; *Evolution, Molecular ; Female ; Gene Expression Regulation/genetics ; Humans ; Male ; Minisatellite Repeats/genetics ; Phenotype ; Species Specificity ; Tandem Repeat Sequences/*genetics ; }, abstract = {Tandem repeats are proposed to contribute to human-specific traits, and more than 40 tandem repeat expansions are known to cause neurological disease. Here, we characterize a human-specific 69 bp variable number tandem repeat (VNTR) in the last intron of WDR7, which exhibits striking variability in both copy number and nucleotide composition, as revealed by long-read sequencing. In addition, greater repeat copy number is significantly enriched in three independent cohorts of individuals with sporadic amyotrophic lateral sclerosis (ALS). Each unit of the repeat forms a stem-loop structure with the potential to produce microRNAs, and the repeat RNA can aggregate when expressed in cells. We leveraged its remarkable sequence variability to align the repeat in 288 samples and uncover its mechanism of expansion. We found that the repeat expands in the 3'-5' direction, in groups of repeat units divisible by two. The expansion patterns we observed were consistent with duplication events, and a replication error called template switching. We also observed that the VNTR is expanded in both Denisovan and Neanderthal genomes but is fixed at one copy or fewer in non-human primates. Evaluating the repeat in 1000 Genomes Project samples reveals that some repeat segments are solely present or absent in certain geographic populations. The large size of the repeat unit in this VNTR, along with our multiplexed sequencing strategy, provides an unprecedented opportunity to study mechanisms of repeat expansion, and a framework for evaluating the roles of VNTRs in human evolution and disease.}, }
@article {pmid32745133, year = {2020}, author = {Örd, T and Puurand, T and Örd, D and Annilo, T and Möls, M and Remm, M and Örd, T}, title = {A human-specific VNTR in the TRIB3 promoter causes gene expression variation between individuals.}, journal = {PLoS genetics}, volume = {16}, number = {8}, pages = {e1008981}, pmid = {32745133}, issn = {1553-7404}, support = {HHSN268201000029C/HL/NHLBI NIH HHS/United States ; HHSN261200800001E/CA/NCI NIH HHS/United States ; }, mesh = {Cell Cycle Proteins/*genetics ; Estonia/epidemiology ; Female ; Gene Expression Regulation/genetics ; *Genetic Heterogeneity ; *Genetics, Population ; Genotype ; Humans ; Male ; Minisatellite Repeats/*genetics ; Promoter Regions, Genetic ; Protein-Serine-Threonine Kinases/*antagonists &amp; inhibitors/genetics ; RNA-Seq ; Repressor Proteins/*genetics ; Whole Genome Sequencing ; }, abstract = {Tribbles homolog 3 (TRIB3) is pseudokinase involved in intracellular regulatory processes and has been implicated in several diseases. In this article, we report that human TRIB3 promoter contains a 33-bp variable number tandem repeat (VNTR) and characterize the heterogeneity and function of this genetic element. Analysis of human populations around the world uncovered the existence of alleles ranging from 1 to 5 copies of the repeat, with 2-, 3- and 5-copy alleles being the most common but displaying considerable geographical differences in frequency. The repeated sequence overlaps a C/EBP-ATF transcriptional regulatory element and is highly conserved, but not repeated, in various mammalian species, including great apes. The repeat is however evident in Neanderthal and Denisovan genomes. Reporter plasmid experiments in human cell culture reveal that an increased copy number of the TRIB3 promoter 33-bp repeat results in increased transcriptional activity. In line with this, analysis of whole genome sequencing and RNA-Seq data from human cohorts demonstrates that the copy number of TRIB3 promoter 33-bp repeats is positively correlated with TRIB3 mRNA expression level in many tissues throughout the body. Moreover, the copy number of the TRIB3 33-bp repeat appears to be linked to known TRIB3 eQTL SNPs as well as TRIB3 SNPs reported in genetic association studies. Taken together, the results indicate that the promoter 33-bp VNTR constitutes a causal variant for TRIB3 expression variation between individuals and could underlie the results of SNP-based genetic studies.}, }
@article {pmid32603726, year = {2021}, author = {Agrawal, R and Testi, I and Bodaghi, B and Barisani-Asenbauer, T and McCluskey, P and Agarwal, A and Kempen, JH and Gupta, A and Smith, JR and de Smet, MD and Yuen, YS and Mahajan, S and Kon, OM and Nguyen, QD and Pavesio, C and Gupta, V and , }, title = {Collaborative Ocular Tuberculosis Study Consensus Guidelines on the Management of Tubercular Uveitis-Report 2: Guidelines for Initiating Antitubercular Therapy in Anterior Uveitis, Intermediate Uveitis, Panuveitis, and Retinal Vasculitis.}, journal = {Ophthalmology}, volume = {128}, number = {2}, pages = {277-287}, doi = {10.1016/j.ophtha.2020.06.052}, pmid = {32603726}, issn = {1549-4713}, abstract = {TOPIC: The Collaborative Ocular Tuberculosis Study (COTS), supported by the International Ocular Inflammation Society, International Uveitis Study Group, and Foster Ocular Immunological Society, set up an international, expert-led consensus project to develop evidence- and experience-based guidelines for the management of tubercular uveitis (TBU).<br><br>CLINICAL RELEVANCE: The absence of international agreement on the use of antitubercular therapy (ATT) in patients with TBU contributes to a significant heterogeneity in the approach to the management of this condition.<br><br>METHODS: Consensus statements for the initiation of ATT in TBU were generated using a 2-step modified Delphi technique. In Delphi step 1, a smart web-based survey based on background evidence from published literature was prepared to collect the opinion of 81 international experts on the use of ATT in different clinical scenarios. The survey included 324 questions related to tubercular anterior uveitis (TAU), tubercular intermediate uveitis (TIU), tubercular panuveitis (TPU), and tubercular retinal vasculitis (TRV) administered by the experts, after which the COTS group met in November 2019 for a systematic and critical discussion of the statements in accordance with the second round of the modified Delphi process.<br><br>RESULTS: Forty-four consensus statements on the initiation of ATT in TAU, TIU, TPU, and TRV were obtained, based on ocular phenotypes suggestive of TBU and corroborative evidence of tuberculosis, provided by several combinations of immunologic and radiologic test results. Experts agreed on initiating ATT in recurrent TAU, TIU, TPU, and active TRV depending on the TB endemicity. In the presence of positive results for any 1 of the immunologic tests along with radiologic features suggestive of past evidence of tuberculosis infection. In patients with a first episode of TAU, consensus to initiate ATT was reached only if both immunologic and radiologic test results were positive.<br><br>DISCUSSION: The COTS consensus guidelines were generated based on the evidence from published literature, specialists' opinions, and logic construction to address the initiation of ATT in TBU. The guidelines also should inform public policy by adding specific types of TBU to the list of conditions that should be treated as tuberculosis.}, }
@article {pmid32580520, year = {2020}, author = {Filippenkov, IB and Stavchansky, VV and Denisova, AE and Yuzhakov, VV and Sevan'kaeva, LE and Sudarkina, OY and Dmitrieva, VG and Gubsky, LV and Myasoedov, NF and Limborska, SA and Dergunova, LV}, title = {Novel Insights into the Protective Properties of ACTH(4-7)PGP (Semax) Peptide at the Transcriptome Level Following Cerebral Ischaemia-Reperfusion in Rats.}, journal = {Genes}, volume = {11}, number = {6}, pages = {}, pmid = {32580520}, issn = {2073-4425}, mesh = {Adrenocorticotropic Hormone/*analogs &amp; derivatives/pharmacology ; Animals ; Brain/drug effects/pathology ; Brain Ischemia/*drug therapy/genetics/pathology ; Disease Models, Animal ; Humans ; Infarction, Middle Cerebral Artery/*drug therapy/genetics/pathology ; Peptide Fragments/*pharmacology ; RNA-Seq ; Rats ; Reperfusion Injury/*drug therapy/genetics/pathology ; Transcriptome/drug effects/genetics ; }, abstract = {Cerebral ischaemia is the most common cause of impaired brain function. Biologically active peptides represent potential drugs for reducing the damage that occurs after ischaemia. The synthetic melanocortin derivative, ACTH(4-7)PGP (Semax), has been used successfully in the treatment of patients with severe impairment of cerebral blood circulation. However, its molecular mechanisms of action within the brain are not yet fully understood. Previously, we used the transient middle cerebral artery occlusion (tMCAO) model to study the damaging effects of ischaemia-reperfusion on the brain transcriptome in rats. Here, using RNA-Seq analysis, we investigated the protective properties of the Semax peptide at the transcriptome level under tMCAO conditions. We have identified 394 differentially expressed genes (DEGs) (>1.5-fold change) in the brains of rats at 24 h after tMCAO treated with Semax relative to saline. Following tMCAO, we found that Semax suppressed the expression of genes related to inflammatory processes and activated the expression of genes related to neurotransmission. In contrast, ischaemia-reperfusion alone activated the expression of inflammation-related genes and suppressed the expression of neurotransmission-related genes. Therefore, the neuroprotective action of Semax may be associated with a compensation of mRNA expression patterns that are disrupted during ischaemia-reperfusion conditions.}, }
@article {pmid32574964, year = {2020}, author = {Mathov, Y and Batyrev, D and Meshorer, E and Carmel, L}, title = {Harnessing epigenetics to study human evolution.}, journal = {Current opinion in genetics &amp; development}, volume = {62}, number = {}, pages = {23-29}, doi = {10.1016/j.gde.2020.05.023}, pmid = {32574964}, issn = {1879-0380}, mesh = {*DNA Methylation ; *Epigenesis, Genetic ; *Evolution, Molecular ; *Genetic Variation ; *Genome, Human ; Humans ; }, abstract = {Recent advances in ancient DNA extraction and high-throughput sequencing technologies enabled the high-quality sequencing of archaic genomes, including the Neanderthal and the Denisovan. While comparisons with modern humans revealed both archaic-specific and human-specific sequence changes, in the absence of gene expression information, understanding the functional implications of such genetic variations remains a major challenge. To study gene regulation in archaic humans, epigenetic research comes to our aid. DNA methylation, which is highly correlated with transcription, can be directly measured in modern samples, as well as reconstructed in ancient samples. This puts DNA methylation as a natural basis for comparative epigenetics between modern humans, archaic humans and nonhuman primates.}, }
@article {pmid32571487, year = {2020}, author = {Kim, J and Perkins, GB and Coates, PT}, title = {Evolutionary immunology: how your ancestry can affect your kidney transplant.}, journal = {Kidney international}, volume = {98}, number = {1}, pages = {45-47}, doi = {10.1016/j.kint.2020.01.020}, pmid = {32571487}, issn = {1523-1755}, mesh = {Alleles ; Animals ; Biological Evolution ; Humans ; *Kidney Transplantation ; Mice ; Phosphorylation ; Tumor Necrosis Factor alpha-Induced Protein 3 ; }, }
@article {pmid32556248, year = {2020}, author = {Khan, N and de Manuel, M and Peyregne, S and Do, R and Prufer, K and Marques-Bonet, T and Varki, N and Gagneux, P and Varki, A}, title = {Multiple Genomic Events Altering Hominin SIGLEC Biology and Innate Immunity Predated the Common Ancestor of Humans and Archaic Hominins.}, journal = {Genome biology and evolution}, volume = {12}, number = {7}, pages = {1040-1050}, pmid = {32556248}, issn = {1759-6653}, support = {R01 GM032373/GM/NIGMS NIH HHS/United States ; U01 MH106874/MH/NIMH NIH HHS/United States ; /HHMI/Howard Hughes Medical Institute/United States ; }, abstract = {Human-specific pseudogenization of the CMAH gene eliminated the mammalian sialic acid (Sia) Neu5Gc (generating an excess of its precursor Neu5Ac), thus changing ubiquitous cell surface "self-associated molecular patterns" that modulate innate immunity via engagement of CD33-related-Siglec receptors. The Alu-fusion-mediated loss-of-function of CMAH fixed ∼2-3 Ma, possibly contributing to the origins of the genus Homo. The mutation likely altered human self-associated molecular patterns, triggering multiple events, including emergence of human-adapted pathogens with strong preference for Neu5Ac recognition and/or presenting Neu5Ac-containing molecular mimics of human glycans, which can suppress immune responses via CD33-related-Siglec engagement. Human-specific alterations reported in some gene-encoding Sia-sensing proteins suggested a "hotspot" in hominin evolution. The availability of more hominid genomes including those of two extinct hominins now allows full reanalysis and evolutionary timing. Functional changes occur in 8/13 members of the human genomic cluster encoding CD33-related Siglecs, all predating the human common ancestor. Comparisons with great ape genomes indicate that these changes are unique to hominins. We found no evidence for strong selection after the Human-Neanderthal/Denisovan common ancestor, and these extinct hominin genomes include almost all major changes found in humans, indicating that these changes in hominin sialobiology predate the Neanderthal-human divergence ∼0.6 Ma. Multiple changes in this genomic cluster may also explain human-specific expression of CD33rSiglecs in unexpected locations such as amnion, placental trophoblast, pancreatic islets, ovarian fibroblasts, microglia, Natural Killer(NK) cells, and epithelia. Taken together, our data suggest that innate immune interactions with pathogens markedly altered hominin Siglec biology between 0.6 and 2 Ma, potentially affecting human evolution.}, }
@article {pmid32546518, year = {2020}, author = {Mafessoni, F and Grote, S and de Filippo, C and Slon, V and Kolobova, KA and Viola, B and Markin, SV and Chintalapati, M and Peyrégne, S and Skov, L and Skoglund, P and Krivoshapkin, AI and Derevianko, AP and Meyer, M and Kelso, J and Peter, B and Prüfer, K and Pääbo, S}, title = {A high-coverage Neandertal genome from Chagyrskaya Cave.}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {117}, number = {26}, pages = {15132-15136}, pmid = {32546518}, issn = {1091-6490}, mesh = {Animals ; Biological Evolution ; Female ; Fossils ; Gene Expression Regulation ; Genetic Variation ; *Genome ; Humans ; Inbreeding ; Neanderthals/*genetics ; Population Density ; Russia ; }, abstract = {We sequenced the genome of a Neandertal from Chagyrskaya Cave in the Altai Mountains, Russia, to 27-fold genomic coverage. We show that this Neandertal was a female and that she was more related to Neandertals in western Eurasia [Prüfer et al., Science 358, 655-658 (2017); Hajdinjak et al., Nature 555, 652-656 (2018)] than to Neandertals who lived earlier in Denisova Cave [Prüfer et al., Nature 505, 43-49 (2014)], which is located about 100 km away. About 12.9% of the Chagyrskaya genome is spanned by homozygous regions that are between 2.5 and 10 centiMorgans (cM) long. This is consistent with the fact that Siberian Neandertals lived in relatively isolated populations of less than 60 individuals. In contrast, a Neandertal from Europe, a Denisovan from the Altai Mountains, and ancient modern humans seem to have lived in populations of larger sizes. The availability of three Neandertal genomes of high quality allows a view of genetic features that were unique to Neandertals and that are likely to have been at high frequency among them. We find that genes highly expressed in the striatum in the basal ganglia of the brain carry more amino-acid-changing substitutions than genes expressed elsewhere in the brain, suggesting that the striatum may have evolved unique functions in Neandertals.}, }
@article {pmid32531199, year = {2020}, author = {Almarri, MA and Bergström, A and Prado-Martinez, J and Yang, F and Fu, B and Dunham, AS and Chen, Y and Hurles, ME and Tyler-Smith, C and Xue, Y}, title = {Population Structure, Stratification, and Introgression of Human Structural Variation.}, journal = {Cell}, volume = {182}, number = {1}, pages = {189-199.e15}, doi = {10.1016/j.cell.2020.05.024}, pmid = {32531199}, issn = {1097-4172}, support = {/WT_/Wellcome Trust/United Kingdom ; FC001595/CRUK_/Cancer Research UK/United Kingdom ; FC001595/MRC_/Medical Research Council/United Kingdom ; FC001595/WT_/Wellcome Trust/United Kingdom ; }, mesh = {Alleles ; Databases, Genetic ; Gene Dosage ; Gene Duplication ; Gene Frequency/genetics ; Genetic Variation ; *Genetics, Population ; Genome, Human ; *Genomic Structural Variation ; Humans ; }, abstract = {Structural variants contribute substantially to genetic diversity and are important evolutionarily and medically, but they are still understudied. Here we present a comprehensive analysis of structural variation in the Human Genome Diversity panel, a high-coverage dataset of 911 samples from 54 diverse worldwide populations. We identify, in total, 126,018 variants, 78% of which were not identified in previous global sequencing projects. Some reach high frequency and are private to continental groups or even individual populations, including regionally restricted runaway duplications and putatively introgressed variants from archaic hominins. By de novo assembly of 25 genomes using linked-read sequencing, we discover 1,643 breakpoint-resolved unique insertions, in aggregate accounting for 1.9 Mb of sequence absent from the GRCh38 reference. Our results illustrate the limitation of a single human reference and the need for high-quality genomes from diverse populations to fully discover and understand human genetic variation.}, }
@article {pmid32494067, year = {2020}, author = {Skov, L and Coll Macià, M and Sveinbjörnsson, G and Mafessoni, F and Lucotte, EA and Einarsdóttir, MS and Jonsson, H and Halldorsson, B and Gudbjartsson, DF and Helgason, A and Schierup, MH and Stefansson, K}, title = {The nature of Neanderthal introgression revealed by 27,566 Icelandic genomes.}, journal = {Nature}, volume = {582}, number = {7810}, pages = {78-83}, pmid = {32494067}, issn = {1476-4687}, mesh = {Animals ; Female ; Genetic Association Studies ; Genetic Introgression/*genetics ; Genome, Human/*genetics ; *Genomics ; Haploidy ; Humans ; Iceland ; Male ; *Mutation ; Neanderthals/*genetics ; Phenotype ; Phylogeny ; }, abstract = {Human evolutionary history is rich with the interbreeding of divergent populations. Most humans outside of Africa trace about 2% of their genomes to admixture from Neanderthals, which occurred 50-60 thousand years ago1. Here we examine the effect of this event using 14.4 million putative archaic chromosome fragments that were detected in fully phased whole-genome sequences from 27,566 Icelanders, corresponding to a range of 56,388-112,709 unique archaic fragments that cover 38.0-48.2% of the callable genome. On the basis of the similarity with known archaic genomes, we assign 84.5% of fragments to an Altai or Vindija Neanderthal origin and 3.3% to Denisovan origin; 12.2% of fragments are of unknown origin. We find that Icelanders have more Denisovan-like fragments than expected through incomplete lineage sorting. This is best explained by Denisovan gene flow, either into ancestors of the introgressing Neanderthals or directly into humans. A within-individual, paired comparison of archaic fragments with syntenic non-archaic fragments revealed that, although the overall rate of mutation was similar in humans and Neanderthals during the 500 thousand years that their lineages were separate, there were differences in the relative frequencies of mutation types-perhaps due to different generation intervals for males and females. Finally, we assessed 271 phenotypes, report 5 associations driven by variants in archaic fragments and show that the majority of previously reported associations are better explained by non-archaic variants.}, }
@article {pmid32487809, year = {2020}, author = {Denisova, K and Barmettler, A}, title = {Oculoplastic considerations for refractive procedures.}, journal = {Current opinion in ophthalmology}, volume = {31}, number = {4}, pages = {241-246}, doi = {10.1097/ICU.0000000000000667}, pmid = {32487809}, issn = {1531-7021}, mesh = {Astigmatism/surgery ; Blepharoptosis/etiology/*physiopathology ; Dry Eye Syndromes/etiology/*physiopathology ; Humans ; Keratomileusis, Laser In Situ ; Keratoplasty, Penetrating ; Orbital Diseases/etiology/*physiopathology ; Postoperative Period ; Refraction, Ocular/physiology ; Refractive Errors/*physiopathology ; *Refractive Surgical Procedures ; }, abstract = {PURPOSE OF REVIEW: Refractive surgery is one of the most popular elective procedures performed in the world. Given that dry eye is a common complaint following keratorefractive surgery, evaluation, and treatment of periocular conditions that further predispose the patient to dry eye symptoms is an important part of the presurgical assessment. Periocular conditions and surgeries can also affect the ocular surface and keratometry, and should be addressed. For example, ptosis, orbital fat herniation, ectropion, and eyelid masses have been shown to induce corneal topography changes and astigmatism. The oculoplastic considerations for refractive surgery include both the contribution of eyelid position on dry eye, ocular surface damage, refractive error, and outcomes, as well as the timing of oculoplastic surgery in relation to the refractive surgery. In this review, the recently published literature on eyelid and orbital surgery in relation to keratorefractive surgery is reviewed to elucidate the relationship of periocular factors with refractive surgery outcomes and complications. To improve keratorefractive surgery outcomes, a literature review is presented, discussing evaluation, management, and timing of management of oculoplastics conditions.<br><br>RECENT FINDINGS: Dry eye syndrome is a well known complication of keratorefractive procedures. This is exacerbated with concurrent eyelid or orbital disorders, such as ectropion, lagophthalmos, and thyroid eye disease. In addition to impacting dry eye and ocular surface damage, eyelid surgeries can also affect corneal topography and refraction. Studies have found that patients with ptosis have topographic corneal aberrations from the eyelid exerting pressure on the cornea, while ptosis repair and blepharoplasty patients may undergo an astigmatic change postoperatively. Finally, the corneal flap created in laser-assisted in situ keratomileusis may be at risk for displacement or damage postoperatively with this risk changing, depending on method of flap creation, and time elapsed since keratorefractive surgery.<br><br>SUMMARY: Eyelid and orbital conditions that predispose to dry eye syndrome and refractive changes should be evaluated and optimized prior to keratorefractive surgery. Patients electing to have oculoplastic surgery, like ptosis repair, should be fully healed prior to any refractive surgery to allow both refractive changes and eyelid positions to stabilize prior to the refractive surgery.}, }
@article {pmid32375623, year = {2020}, author = {Larina, VN and Akhmatova, FD and Arakelov, SE and Mokhov, AE and Doronina, IM and Denisova, NN}, title = {[Modern strategies for cardiac rehabilitation after myocardial infarction and percutaneous coronary intervention].}, journal = {Kardiologiia}, volume = {60}, number = {3}, pages = {111-118}, doi = {10.18087/cardio.2020.3.n546}, pmid = {32375623}, issn = {0022-9040}, mesh = {*Cardiac Rehabilitation ; Exercise ; Exercise Therapy ; Humans ; *Myocardial Infarction ; *Percutaneous Coronary Intervention ; }, abstract = {Modern cardiac rehabilitation represents a structured, multicomponent program, which includes physical activity, education of the patient, modification of the health behavior, and psychological and social support. In EU countries, only 44.8% of patients with ischemic heart disease receive a recommendation to participate in any form of rehabilitation, and only 36.5% of all patients presently have an access to any rehabilitation program. Systematic analysis of programs for prevention of cardiovascular diseases and for rehabilitation in patients with myocardial infarction (MI) and percutaneous coronary intervention showed that complex programs can still reduce all-cause and cardiovascular mortality and frequency of recurrent MI and stroke. These programs include key components of cardiac rehabilitation, reduction of six or more risk factors, and effective control by drug therapy.}, }
@article {pmid32369667, year = {2020}, author = {Denisova, SA and Shchenkov, SV}, title = {Fine structure of the nervous system of Cercaria parvicaudata Stunkard &amp; Shaw, 1931 (Digenea, Renicolidae).}, journal = {Journal of morphology}, volume = {281}, number = {7}, pages = {765-777}, doi = {10.1002/jmor.21137}, pmid = {32369667}, issn = {1097-4687}, mesh = {Animals ; Cercaria/*anatomy &amp; histology/ultrastructure ; Ganglia, Invertebrate/ultrastructure ; Nervous System/*anatomy &amp; histology/ultrastructure ; Neurons/ultrastructure ; Neuropil Threads/ultrastructure ; Synapses/ultrastructure ; }, abstract = {The biology of free-living and parasitic Platyhelminthes is diverse. Taking into account the widespread prevalence of parasitic flatworms, Digenea is the least studied group regarding the fine structure of nervous system especially of the cercarial life stage. Here, we present a description of the fine structure of central nervous system (CNS) and two types of uniciliate sensory papillae of xiphidiocercaria Cercaria parvicaudata (Microphalloidea, Renicolidae). The present study documents that C. parvicaudata has a complex nervous system that includes a well-developed ganglion with a cortex of perikarya and glia-like sheaths, myelin-like structures within one of the dorsal nerve cords and four types of polarized synapses between neurites. Different types of neurons in the CNS could not be distinguished on ultrastructural level due to high similarity in their fine structure. Shared polarized synapses with high electron density of presynaptic components are numerous in the neuropile and nerve cords of this larva. Within the larval body, we detected specialized "support" processes that relate to different tissues. Some "support" processes are also closely related to the nervous system of C. parvicaudata, where they are considered as glia-like structures. In this case, the fine structure of glia-like "support" cells of C. parvicaudata differs from those described as glia-like cells in adult flatworms. We suggest a wide prevalence of glia-like cells among cercariae, as well as the fact that glia-like structures in digenean nervous systems can develop from various nonneuronal tissues.}, }
@article {pmid32368616, year = {2020}, author = {Hammill, JA and Kwiecien, JM and Dvorkin-Gheva, A and Lau, VWC and Baker, C and Wu, Y and Bezverbnaya, K and Aarts, C and Heslen, CW and Denisova, GF and Derocher, H and Milne, K and Nelson, BH and Bramson, JL}, title = {A Cross-Reactive Small Protein Binding Domain Provides a Model to Study Off-Tumor CAR-T Cell Toxicity.}, journal = {Molecular therapy oncolytics}, volume = {17}, number = {}, pages = {278-292}, pmid = {32368616}, issn = {2372-7705}, abstract = {Tumor-targeted chimeric antigen receptor (CAR)-engineered T lymphocytes (CAR-T cells) have demonstrated striking clinical success, but their use has been associated with a constellation of toxicities. A better understanding of the pathogenesis of these toxicities is required to improve the safety profile of CAR-T cells. Herein, we describe a xenograft model of off-tumor CAR-T cell-associated toxicity. Human CAR-T cells targeted against HER2 using a small-protein binding domain induced acute, dose-dependent toxicities in mice. The inclusion of a CD28 or 4-1BB co-stimulatory domain in the CAR was required to produce toxicity; however, co-stimulation through CD28 was most toxic on a per-cell basis. CAR-T cell activation in the lungs and heart was associated with a systemic cytokine storm. The severity of observed toxicities was dependent upon the peripheral blood mononuclear cell (PBMC) donor used as a T cell source and paralleled the CD4+-to-CD8+ T cell ratio in the adoptive transfer product. CD4+ CAR-T cells were determined to be the primary contributors to CAR-T cell-associated toxicity. However, donor-specific differences persisted after infusion of a purified CD4+ CAR-T cell product, indicating a role for additional variables. This work highlights the contributions of CAR-T cell-intrinsic variables to the pathogenesis of off-tumor toxicity.}, }
@article {pmid32330268, year = {2020}, author = {Lodewijk, GA and Fernandes, DP and Vretzakis, I and Savage, JE and Jacobs, FMJ}, title = {Evolution of Human Brain Size-Associated NOTCH2NL Genes Proceeds toward Reduced Protein Levels.}, journal = {Molecular biology and evolution}, volume = {37}, number = {9}, pages = {2531-2548}, pmid = {32330268}, issn = {1537-1719}, mesh = {Animals ; *Biological Evolution ; Genome, Human ; Genomic Structural Variation ; Humans ; Multigene Family ; Neanderthals/*genetics ; Receptor, Notch2/*genetics/metabolism ; }, abstract = {Ever since the availability of genomes from Neanderthals, Denisovans, and ancient humans, the field of evolutionary genomics has been searching for protein-coding variants that may hold clues to how our species evolved over the last ∼600,000 years. In this study, we identify such variants in the human-specific NOTCH2NL gene family, which were recently identified as possible contributors to the evolutionary expansion of the human brain. We find evidence for the existence of unique protein-coding NOTCH2NL variants in Neanderthals and Denisovans which could affect their ability to activate Notch signaling. Furthermore, in the Neanderthal and Denisovan genomes, we find unusual NOTCH2NL configurations, not found in any of the modern human genomes analyzed. Finally, genetic analysis of archaic and modern humans reveals ongoing adaptive evolution of modern human NOTCH2NL genes, identifying three structural variants acting complementary to drive our genome to produce a lower dosage of NOTCH2NL protein. Because copy-number variations of the 1q21.1 locus, encompassing NOTCH2NL genes, are associated with severe neurological disorders, this seemingly contradicting drive toward low levels of NOTCH2NL protein indicates that the optimal dosage of NOTCH2NL may have not yet been settled in the human population.}, }
@article {pmid32264696, year = {2020}, author = {Kurmukov, A and Mussabaeva, A and Denisova, Y and Moyer, D and Jahanshad, N and Thompson, PM and Gutman, BA}, title = {Optimizing Connectivity-Driven Brain Parcellation Using Ensemble Clustering.}, journal = {Brain connectivity}, volume = {10}, number = {4}, pages = {183-194}, pmid = {32264696}, issn = {2158-0022}, support = {P41 EB015922/EB/NIBIB NIH HHS/United States ; R01 MH116147/MH/NIMH NIH HHS/United States ; U01 AG024904/AG/NIA NIH HHS/United States ; U54 EB020403/EB/NIBIB NIH HHS/United States ; }, mesh = {Adult ; Atlases as Topic ; Brain/*anatomy &amp; histology/diagnostic imaging ; Connectome ; Diffusion Magnetic Resonance Imaging/*methods/standards ; Female ; Humans ; Image Interpretation, Computer-Assisted/methods ; Image Processing, Computer-Assisted/methods ; Male ; Nerve Net/*anatomy &amp; histology/diagnostic imaging ; Neuroimaging/*methods/standards ; Young Adult ; }, abstract = {This work addresses the problem of constructing a unified, topologically optimal connectivity-based brain atlas. The proposed approach aggregates an ensemble partition from individual parcellations without label agreement, providing a balance between sufficiently flexible individual parcellations and intuitive representation of the average topological structure of the connectome. The methods exploit a previously proposed dense connectivity representation, first performing graph-based hierarchical parcellation of individual brains, and subsequently aggregating the individual parcellations into a consensus parcellation. The search for consensus-based on the hard ensemble (HE) algorithm-approximately minimizes the sum of cluster membership distances, effectively estimating a pseudo-Karcher mean of individual parcellations. Computational stability, graph structure preservation, and biological relevance of the simplified representation resulting from the proposed parcellation are assessed on the Human Connectome Project data set. These aspects are assessed using (1) edge weight distribution divergence with respect to the dense connectome representation, (2) interhemispheric symmetry, (3) network characteristics' stability and agreement with respect to individually and anatomically parcellated networks, and (4) performance of the simplified connectome in a biological sex classification task. Ensemble parcellation was found to be highly stable with respect to subject sampling, outperforming anatomical atlases and other connectome-based parcellations in classification as well as preserving global connectome properties. The HE-based parcellation also showed a degree of symmetry comparable with anatomical atlases and a high degree of spatial contiguity without using explicit priors.}, }
@article {pmid32250731, year = {2020}, author = {Agrawal, R and Testi, I and Mahajan, S and Yuen, YS and Agarwal, A and Rousselot, A and Raje, D and Gunasekeran, DV and Kon, OM and Barisani-Asenbauer, T and Kempen, JH and Gupta, A and Jabs, DA and Smith, JR and Bodaghi, B and Zierhut, M and DeSmet, M and Cluskey, PM and Agarwal, M and Agarwal, M and Aggarwal, K and Agrawal, M and Al-Dhibi, H and Androudi, S and Asyari, F and Balasundaram, MB and Murthy, KB and Baglivo, E and Banker, A and Bansal, R and Basu, S and Behera, D and Biswas, J and Carreño, E and Caspers, L and Chee, SP and Chhabra, R and Cimino, L and Del Rio, LEC and Cunningham, ET and Curi, ALL and Das, D and Denisova, E and Denniston, AK and Errera, MH and Fonollosa, A and George, A and Goldstein, DA and Crosier, YG and Gurbaxani, A and Invernizzi, A and Isa, HM and Md Islam, S and Jones, N and Katoch, D and Khairallah, M and Khosla, A and Kramer, M and Kumar, A and Kumar, A and Nora, RD and Lee, R and Lowder, C and Luthra, S and Mahendradas, P and Makhoul, D and Mazumdar, S and Mehta, S and Miserocchi, E and Mochizuki, M and Mohamed, OS and Muccioli, C and Munk, MR and Murthy, S and Narain, S and Nascimento, H and Neri, P and Nguyen, M and Okada, AA and Ozdal, P and Palestine, A and Pichi, F and Rathinam, SR and Schlaen, A and Sehgal, S and Sen, HN and Sharma, A and Sharma, K and Shoughy, SS and Singh, N and Singh, R and Soheilian, M and Sridharan, S and Thorne, JE and Tappeiner, C and Teoh, S and Tognon, MS and Tugal-Tutkun, I and Tyagi, M and Uy, H and Santos, DVV and Valentincic, NV and Westcott, M and Yanai, R and Alvarez, BY and Zahedur, R and Nguyen, QD and Pavesio, C and Gupta, V}, title = {The Collaborative Ocular Tuberculosis Study (COTS) Consensus (CON) Group Meeting Proceedings.}, journal = {Ocular immunology and inflammation}, volume = {}, number = {}, pages = {1-11}, doi = {10.1080/09273948.2020.1716025}, pmid = {32250731}, issn = {1744-5078}, abstract = {An international, expert led consensus initiative was set up by the Collaborative Ocular Tuberculosis Study (COTS) group to develop systematic, evidence, and experience-based recommendations for the treatment of ocular TB using a modified Delphi technique process. In the first round of Delphi, the group identified clinical scenarios pertinent to ocular TB based on five clinical phenotypes (anterior uveitis, intermediate uveitis, choroiditis, retinal vasculitis, and panuveitis). Using an interactive online questionnaires, guided by background knowledge from published literature, 486 consensus statements for initiating ATT were generated and deliberated amongst 81 global uveitis experts. The median score of five was considered reaching consensus for initiating ATT. The median score of four was tabled for deliberation through Delphi round 2 in a face-to-face meeting. This report describes the methodology adopted and followed through the consensus process, which help elucidate the guidelines for initiating ATT in patients with choroidal TB.}, }
@article {pmid32201812, year = {2020}, author = {Mitoudi Vagourdi, E and Zhang, W and Denisova, K and Lemmens, P and Halasyamani, PS and Johnsson, M}, title = {Synthesis and Characterization of Two New Second Harmonic Generation Active Iodates: K3Sc(IO3)6 and KSc(IO3)3Cl.}, journal = {ACS omega}, volume = {5}, number = {10}, pages = {5235-5240}, pmid = {32201812}, issn = {2470-1343}, abstract = {Transparent single crystals of two new iodates K3Sc(IO3)6 and KSc(IO3)3Cl have been synthesized hydrothermally. Single-crystal X-ray diffraction was used to determine their crystal structures. Both compounds crystallize in non-centrosymmetric space groups. The compound K3Sc(IO3)6 crystallizes in the orthorhombic space group Fdd2. The crystal structure is made up of [ScO6] octahedra, [IO3] trigonal pyramids, and [KO8] distorted cubes. The compound KSc(IO3)3Cl crystallizes in the trigonal space group R3. The building blocks are [ScO6] octahedra, [KO12] polyhedra, and [IO3] trigonal pyramids. The Cl- ions act as counter ions and reside in tunnels in the crystal structure. The second harmonic generation (SHG) measurements at room temperature, using 1064 nm radiation, on polycrystalline samples show that the SHG intensities of K3Sc(IO3)6 and KSc(IO3)3Cl are around 2.8 and 2.5 times that of KH2PO4 (KDP), respectively. In addition, K3Sc(IO3)6 and KSc(IO3)3Cl are phase-matchable at the fundamental wavelength of 1064 nm. The large anharmonicity in the optical response of both compounds is further supported by an anomalous temperature dependence of optical phonon frequencies as well as their enlarged intensities in Raman scattering. The latter corresponds to a very large electronic polarizability.}, }
@article {pmid32193295, year = {2020}, author = {Bergström, A and McCarthy, SA and Hui, R and Almarri, MA and Ayub, Q and Danecek, P and Chen, Y and Felkel, S and Hallast, P and Kamm, J and Blanché, H and Deleuze, JF and Cann, H and Mallick, S and Reich, D and Sandhu, MS and Skoglund, P and Scally, A and Xue, Y and Durbin, R and Tyler-Smith, C}, title = {Insights into human genetic variation and population history from 929 diverse genomes.}, journal = {Science (New York, N.Y.)}, volume = {367}, number = {6484}, pages = {}, pmid = {32193295}, issn = {1095-9203}, support = {FC001595/MRC_/Medical Research Council/United Kingdom ; /ERC_/European Research Council/International ; /HHMI/Howard Hughes Medical Institute/United States ; 206194/WT_/Wellcome Trust/United Kingdom ; 098051/WT_/Wellcome Trust/United Kingdom ; //Medical Research Council,/International ; 207492/WT_/Wellcome Trust/United Kingdom ; FC001595/WT_/Wellcome Trust/United Kingdom ; FC001595/CRUK_/Cancer Research UK/United Kingdom ; /WT_/Wellcome Trust/United Kingdom ; }, mesh = {Africa ; Americas ; Animals ; Asia ; Continental Population Groups/genetics ; DNA Copy Number Variations ; *Genetic Variation ; *Genetics, Population ; *Genome, Human ; Haplotypes ; Hominidae/genetics ; Humans ; INDEL Mutation ; Neanderthals/genetics ; Oceania ; Phylogeny ; Polymorphism, Single Nucleotide ; Population Density ; *Whole Genome Sequencing ; }, abstract = {Genome sequences from diverse human groups are needed to understand the structure of genetic variation in our species and the history of, and relationships between, different populations. We present 929 high-coverage genome sequences from 54 diverse human populations, 26 of which are physically phased using linked-read sequencing. Analyses of these genomes reveal an excess of previously undocumented common genetic variation private to southern Africa, central Africa, Oceania, and the Americas, but an absence of such variants fixed between major geographical regions. We also find deep and gradual population separations within Africa, contrasting population size histories between hunter-gatherer and agriculturalist groups in the past 10,000 years, and a contrast between single Neanderthal but multiple Denisovan source populations contributing to present-day human populations.}, }
@article {pmid32151667, year = {2020}, author = {Stark, MS and Denisova, E and Kays, TA and Heidenreich, B and Rachakonda, S and Requena, C and Sturm, RA and Soyer, HP and Nagore, E and Kumar, R}, title = {Mutation Signatures in Melanocytic Nevi Reveal Characteristics of Defective DNA Repair.}, journal = {The Journal of investigative dermatology}, volume = {140}, number = {10}, pages = {2093-2096.e2}, doi = {10.1016/j.jid.2020.02.021}, pmid = {32151667}, issn = {1523-1747}, mesh = {DNA Copy Number Variations ; *DNA Repair ; Humans ; *Mutation ; Nevus, Pigmented/*genetics ; Polymorphism, Single Nucleotide ; Whole Exome Sequencing ; }, }
@article {pmid32128408, year = {2020}, author = {Rogers, AR and Harris, NS and Achenbach, AA}, title = {Neanderthal-Denisovan ancestors interbred with a distantly related hominin.}, journal = {Science advances}, volume = {6}, number = {8}, pages = {eaay5483}, pmid = {32128408}, issn = {2375-2548}, mesh = {Animals ; Confidence Intervals ; *Consanguinity ; Gene Flow ; Genetics, Population ; Hominidae/*genetics ; Models, Genetic ; Neanderthals/*genetics ; Phylogeny ; }, abstract = {Previous research has shown that modern Eurasians interbred with their Neanderthal and Denisovan predecessors. We show here that hundreds of thousands of years earlier, the ancestors of Neanderthals and Denisovans interbred with their own Eurasian predecessors-members of a "superarchaic" population that separated from other humans about 2 million years ago. The superarchaic population was large, with an effective size between 20 and 50 thousand individuals. We confirm previous findings that (i) Denisovans also interbred with superarchaics, (ii) Neanderthals and Denisovans separated early in the middle Pleistocene, (iii) their ancestors endured a bottleneck of population size, and (iv) the Neanderthal population was large at first but then declined in size. We provide qualified support for the view that (v) Neanderthals interbred with the ancestors of modern humans.}, }
@article {pmid32119213, year = {2020}, author = {Denisova, OA and Gudina, TV and Bukina, IA and Lekhanova, OL and Bukin, DA}, title = {[The medical pedagogical prevention of deviant behavior in adolescents from disadvantaged family in conditions of rural school].}, journal = {Problemy sotsial'noi gigieny, zdravookhraneniia i istorii meditsiny}, volume = {28}, number = {1}, pages = {74-82}, doi = {10.32687/0869-866X-2020-28-1-74-82}, pmid = {32119213}, issn = {0869-866X}, mesh = {Adolescent ; Child ; Humans ; Prevalence ; *Rural Population ; Schools ; *Substance-Related Disorders ; *Vulnerable Populations ; }, abstract = {The article considers medical and pedagogical prevention of deviant behavior of adolescents from dysfunctional families in conditions of rural school, related to the need of strengthening targeted joint interaction of medical and pedagogical institutions. The description and results of testing of diagnostic tools used to establish severity of deviant behavior of children in a selected group is discussed. The interaction of medical and educational institutions in preventing deviant behavior of adolescents from dysfunctional families in rural school is of key importance for combating prevalence of drug addiction, tobacco smoking, alcohol abuse and psychoactive substances consumption, and contributing to physical and mental health promotion of younger generation, development of healthy lifestyle. The study was organized to cover cognitive, behavioral, affective and value-motivational components. The qualitative characteristics of levels of severity of deviant behavior of adolescents from dysfunctional families were developed and proposed for practice application.}, }
@article {pmid32115264, year = {2021}, author = {Agrawal, R and Testi, I and Mahajan, S and Yuen, YS and Agarwal, A and Kon, OM and Barisani-Asenbauer, T and Kempen, JH and Gupta, A and Jabs, DA and Smith, JR and Nguyen, QD and Pavesio, C and Gupta, V and , }, title = {Collaborative Ocular Tuberculosis Study Consensus Guidelines on the Management of Tubercular Uveitis-Report 1: Guidelines for Initiating Antitubercular Therapy in Tubercular Choroiditis.}, journal = {Ophthalmology}, volume = {128}, number = {2}, pages = {266-276}, doi = {10.1016/j.ophtha.2020.01.008}, pmid = {32115264}, issn = {1549-4713}, abstract = {TOPIC: An international, expert-led consensus initiative organized by the Collaborative Ocular Tuberculosis Study (COTS), along with the International Ocular Inflammation Society and the International Uveitis Study Group, systematically developed evidence- and experience-based recommendations for the treatment of tubercular choroiditis.<br><br>CLINICAL RELEVANCE: The diagnosis and management of tubercular uveitis (TBU) pose a significant challenge. Current guidelines and literature are insufficient to guide physicians regarding the initiation of antitubercular therapy (ATT) in patients with TBU.<br><br>METHODS: An international expert steering subcommittee of the COTS group identified clinical questions and conducted a systematic review of the published literature on the use of ATT for tubercular choroiditis. Using an interactive online questionnaire, guided by background knowledge from published literature, 81 global experts (including ophthalmologists, pulmonologists, and infectious disease physicians) generated preliminary consensus statements for initiating ATT in tubercular choroiditis, using Oxford levels of medical evidence. In total, 162 statements were identified regarding when to initiate ATT in patients with tubercular serpiginous-like choroiditis, tuberculoma, and tubercular focal or multifocal choroiditis. The COTS group members met in November 2018 to refine these statements by a 2-step modified Delphi process.<br><br>RESULTS: Seventy consensus statements addressed the initiation of ATT in the 3 subtypes of tubercular choroiditis, and in addition, 10 consensus statements were developed regarding the use of adjunctive therapy in tubercular choroiditis. Experts agreed on initiating ATT in tubercular choroiditis in the presence of positive results for any 1 of the positive immunologic tests along with radiologic features suggestive of tuberculosis. For tubercular serpiginous-like choroiditis and tuberculoma, positive results from even 1 positive immunologic test were considered sufficient to recommend ATT, even if there were no radiologic features suggestive of tuberculosis.<br><br>DISCUSSION: Consensus guidelines were developed to guide the initiation of ATT in patients with tubercular choroiditis, based on the published literature, expert opinion, and practical experience, to bridge the gap between clinical need and available medical evidence.}, }
@article {pmid32067025, year = {2020}, author = {Shlyakhtina, AV and Avdeev, M and Lyskov, NV and Abrantes, JCC and Gomes, E and Denisova, KN and Kolbanev, IV and Chernyak, SA and Volkova, OS and Vasiliev, AN}, title = {Structure, conductivity and magnetism of orthorhombic and fluorite polymorphs in MoO3-Ln2O3 (Ln = Gd, Dy, Ho) systems.}, journal = {Dalton transactions (Cambridge, England : 2003)}, volume = {49}, number = {9}, pages = {2833-2842}, doi = {10.1039/c9dt04724g}, pmid = {32067025}, issn = {1477-9234}, abstract = {Phase-pure orthorhombic compositions at a Ln/Mo ratio ∼ 5.2-5.7 (Ln = Gd, Dy, Ho) have been obtained for the first time by prolonged (40-160 h) heat treatment of mechanically activated 5Ln2O3 + 2MoO3 (Ln = Gd, Dy, Ho) oxide mixtures at 1200 °C. Although the starting Ln : Mo ratio was 5 : 1 (Ln10Mo2O21 (Ln = Dy, Ho)), it changed slightly in the final product due to the volatility of molybdenum oxide at 1200 °C (40-160 h) (ICP-MS analysis). Brief high-temperature firing (1600 °C, 3 h) of 5Ln2O3 + 2MoO3 (Ln = Gd, Dy, Ho) oxide mixtures leads to the formation of phase-pure fluorites with compositions close to Ln10Mo2O21 (Ln = Gd, Dy, Ho). Gd10Mo2O21 molybdate seems to undergo an order-disorder (orthorhombic-fluorite) phase transition in the range of 1200-1600 °C. For the first time, using the neutron diffraction method, it was shown that low-temperature phases with a Ln/Mo ratio ∼ 5.2-5.7 (Ln = Gd, Dy, Ho) have an orthorhombic structure rather than a tetragonal structure. Proton contribution to the total conductivity of Ln10Mo2O21 (Ln = Gd, Dy, Ho) fluorites and gadolinium and dysprosium orthorhombic phases in a wet atmosphere was observed for the first time. In both orthorhombic and fluorite phases, the total conductivity in wet air decreases with decreasing lanthanide ionic radii. In a wide temperature range, the compounds under study exhibit paramagnetic behaviour. However, the orthorhombic phases of Dy and Ho compounds reach the antiferromagnetic state at 2.4 K and 2.6 K, respectively.}, }
@article {pmid32032517, year = {2020}, author = {Gokhman, D and Mishol, N and de Manuel, M and de Juan, D and Shuqrun, J and Meshorer, E and Marques-Bonet, T and Rak, Y and Carmel, L}, title = {Reconstructing Denisovan Anatomy Using DNA Methylation Maps.}, journal = {Cell}, volume = {180}, number = {3}, pages = {601}, doi = {10.1016/j.cell.2020.01.020}, pmid = {32032517}, issn = {1097-4172}, }
@article {pmid31987110, year = {2020}, author = {Rudnev, S and Burns, JS and Williams, PL and Lee, MM and Korrick, SA and Denisova, T and Dikov, Y and Kozupitsa, G and Hauser, R and Sergeyev, O}, title = {Comparison of bioimpedance body composition in young adults in the Russian Children's Study.}, journal = {Clinical nutrition ESPEN}, volume = {35}, number = {}, pages = {153-161}, pmid = {31987110}, issn = {2405-4577}, support = {P30 ES000002/ES/NIEHS NIH HHS/United States ; R01 ES014370/ES/NIEHS NIH HHS/United States ; }, abstract = {BACKGROUND &amp; AIMS: Body mass index is a simple anthropometric measure (kg/m2) used as an indirect estimate of body fat in individuals, and in assessments of population health and comparisons between populations. Bioelectrical impedance analysis (BIA) is often used to provide additional information on body fat and fat-free mass, and has been used to generate body composition reference data in national health surveys. However, BIA measurements are known to be device-specific and there are few published studies comparing results from different BIA instruments. Therefore, we compared the performance of two BIA instruments in the Russian Children's Study (RCS) of male growth, pubertal development and maturation.<br><br>METHODS: Paired BIA measurements were obtained using the Tanita BC-418MA (Tanita Corp., Tokyo, Japan) and ABC-01 'Medas' (Medas Ltd, Moscow, Russia) BIA instruments. Cross-sectional data on 236 RCS subjects aged 18-22 years were used for the BIA comparison and the development of a conversion formula between measured resistances; follow-up data (n = 96) were used for validation of the conversion formula.<br><br>RESULTS: Whole-body resistances were highly correlated (Spearman rho = 0.95), but fat mass (FM) estimates were significantly higher with the Medas than the Tanita device (median difference 3.3 kg, 95% CI: 2.9, 3.6 kg) with large limits of agreement (LoA) for the FM difference (-2.0, 8.6 kg). A conversion formula between the resistances (Res) was obtained: Medas Res = 0.882 × Tanita Res+26.2 (r2 = 0.91, SEE = 17.6 Ohm). After applying the conversion formula to Tanita data and application of the Medas assessment algorithm, the 'converted' Tanita FM estimates closely matched the Medas original estimates (median difference -0.1 kg, 95% CI: -0.3, 0.2 kg), with relatively small LoA for the FM difference (-2.3 to 2.1 kg), suggesting potential interchangeability of the ABC-01 'Medas' and Tanita BC-418MA data at the group level.<br><br>CONCLUSIONS: Our results support the importance of cross-calibration of BIA instruments for population comparisons and proper data interpretation in clinical and epidemiological studies.}, }
@article {pmid31935281, year = {2020}, author = {Gouy, A and Excoffier, L}, title = {Polygenic Patterns of Adaptive Introgression in Modern Humans Are Mainly Shaped by Response to Pathogens.}, journal = {Molecular biology and evolution}, volume = {37}, number = {5}, pages = {1420-1433}, doi = {10.1093/molbev/msz306}, pmid = {31935281}, issn = {1537-1719}, mesh = {Adaptation, Biological/*genetics ; *Genetic Introgression ; Host-Pathogen Interactions/*genetics ; Humans ; Melanesia ; *Multifactorial Inheritance ; *Selection, Genetic ; }, abstract = {Anatomically modern humans carry many introgressed variants from other hominins in their genomes. Some of them affect their phenotype and can thus be negatively or positively selected. Several individual genes have been proposed to be the subject of adaptive introgression, but the possibility of polygenic adaptive introgression has not been extensively investigated yet. In this study, we analyze archaic introgression maps with refined functional enrichment methods to find signals of polygenic adaptation of introgressed variants. We first apply a method to detect sets of connected genes (subnetworks) within biological pathways that present higher-than-expected levels of archaic introgression. We then introduce and apply a new statistical test to distinguish between epistatic and independent selection in gene sets of present-day humans. We identify several known targets of adaptive introgression, and we show that they belong to larger networks of introgressed genes. After correction for genetic linkage, we find that signals of polygenic adaptation are mostly explained by independent and potentially sequential selection episodes. However, we also find some gene sets where introgressed variants present significant signals of epistatic selection. Our results confirm that archaic introgression has facilitated local adaptation, especially in immunity related and metabolic functions and highlight its involvement in a coordinated response to pathogens out of Africa.}, }
@article {pmid31878873, year = {2019}, author = {Bücking, R and Cox, MP and Hudjashov, G and Saag, L and Sudoyo, H and Stoneking, M}, title = {Archaic mitochondrial DNA inserts in modern day nuclear genomes.}, journal = {BMC genomics}, volume = {20}, number = {1}, pages = {1017}, pmid = {31878873}, issn = {1471-2164}, mesh = {Animals ; Cell Nucleus/*genetics ; DNA, Mitochondrial/*genetics ; Evolution, Molecular ; Genomics/*methods ; Hominidae/genetics ; Neanderthals/genetics ; Phylogeny ; }, abstract = {BACKGROUND: Traces of interbreeding of Neanderthals and Denisovans with modern humans in the form of archaic DNA have been detected in the genomes of present-day human populations outside sub-Saharan Africa. Up to now, only nuclear archaic DNA has been detected in modern humans; we therefore attempted to identify archaic mitochondrial DNA (mtDNA) residing in modern human nuclear genomes as nuclear inserts of mitochondrial DNA (NUMTs).<br><br>RESULTS: We analysed 221 high-coverage genomes from Oceania and Indonesia using an approach which identifies reads that map both to the nuclear and mitochondrial DNA. We then classified reads according to the source of the mtDNA, and found one NUMT of Denisovan mtDNA origin, present in 15 analysed genomes; analysis of the flanking region suggests that this insertion is more likely to have happened in a Denisovan individual and introgressed into modern humans with the Denisovan nuclear DNA, rather than in a descendant of a Denisovan female and a modern human male.<br><br>CONCLUSIONS: Here we present our pipeline for detecting introgressed NUMTs in next generation sequencing data that can be used on genomes sequenced in the future. Further discovery of such archaic NUMTs in modern humans can be used to detect interbreeding between archaic and modern humans and can reveal new insights into the nature of such interbreeding events.}, }
@article {pmid31848254, year = {2020}, author = {Scott, GR and Irish, JD and Martinón-Torres, M}, title = {A more comprehensive view of the Denisovan 3-rooted lower second molar from Xiahe.}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {117}, number = {1}, pages = {37-38}, pmid = {31848254}, issn = {1091-6490}, mesh = {Asian Continental Ancestry Group ; *Fossils ; Humans ; Molar/anatomy &amp; histology ; *Tooth ; }, }
@article {pmid31821096, year = {2019}, author = {Agrawal, R and Agarwal, A and Jabs, DA and Kee, A and Testi, I and Mahajan, S and McCluskey, PJ and Gupta, A and Palestine, A and Denniston, A and Banker, A and Invernizzi, A and Fonollosa, A and Sharma, A and Kumar, A and Curi, A and Okada, A and Schlaen, A and Heiligenhaus, A and Kumar, A and Gurbaxani, A and Bodaghi, B and Islam Shah, B and Lowder, C and Tappeiner, C and Muccioli, C and Vasconcelos-Santos, DV and Goldstein, D and Behra, D and Das, D and Makhoul, D and Baglivo, E and Denisova, E and Miserocchi, E and Carreno, E and Asyari, F and Pichi, F and Sen, HN and Uy, H and Nascimento, H and Tugal-Tutkun, I and Arevalo, JF and Davis, J and Thorne, J and Hisae Yamamoto, J and Smith, J and Garweg, JG and Biswas, J and Babu, K and Aggarwal, K and Cimino, L and Kuffova, L and Agarwal, M and Zierhut, M and Agarwal, M and De Smet, M and Tognon, MS and Errera, MH and Munk, M and Westcott, M and Soheilian, M and Accorinti, M and Khairallah, M and Nguyen, M and Kon, OM and Mahendradas, P and Yang, P and Neri, P and Ozdal, P and Amer, R and Lee, R and Distia Nora, R and Chhabra, R and Belfort, R and Mehta, S and Shoughy, S and Luthra, S and Mohamed, SO and Chee, SP and Basu, S and Teoh, S and Ganesh, S and Barisani-Asenbauer, T and Guex-Crosier, Y and Ozyazgan, Y and Akova, Y and Habot-Wilner, Z and Kempen, J and Nguyen, QD and Pavesio, C and Gupta, V and , }, title = {Standardization of Nomenclature for Ocular Tuberculosis - Results of Collaborative Ocular Tuberculosis Study (COTS) Workshop.}, journal = {Ocular immunology and inflammation}, volume = {}, number = {}, pages = {1-11}, doi = {10.1080/09273948.2019.1653933}, pmid = {31821096}, issn = {1744-5078}, support = {ETM/351/CSO_/Chief Scientist Office/United Kingdom ; }, abstract = {Purpose: To standardize a nomenclature system for defining clinical phenotypes, and outcome measures for reporting clinical and research data in patients with ocular tuberculosis (OTB).Methods: Uveitis experts initially administered and further deliberated the survey in an open meeting to determine and propose the preferred nomenclature for terms related to the OTB, terms describing the clinical phenotypes and treatment and reporting outcomes.Results: The group of experts reached a consensus on terming uveitis attributable to tuberculosis (TB) as tubercular uveitis. The working group introduced a SUN-compatible nomenclature that also defines disease "remission" and "cure", both of which are relevant for reporting treatment outcomes.Conclusion: A consensus nomenclature system has been adopted by a large group of international uveitis experts for OTB. The working group recommends the use of standardized nomenclature to prevent ambiguity in communication and to achieve the goal of spreading awareness of this blinding uveitis entity.}, }
@article {pmid31809748, year = {2019}, author = {Wall, JD and Ratan, A and Stawiski, E and , }, title = {Identification of African-Specific Admixture between Modern and Archaic Humans.}, journal = {American journal of human genetics}, volume = {105}, number = {6}, pages = {1254-1261}, pmid = {31809748}, issn = {1537-6605}, support = {P30 CA044579/CA/NCI NIH HHS/United States ; R01 GM115433/GM/NIGMS NIH HHS/United States ; }, mesh = {African Continental Ancestry Group/*genetics ; Animals ; *Fossils ; Gene Pool ; *Genetics, Population ; *Genome, Human ; Hominidae/*genetics ; Humans ; Neanderthals/*genetics ; }, abstract = {Recent work has demonstrated that two archaic human groups (Neanderthals and Denisovans) interbred with modern humans and contributed to the contemporary human gene pool. These findings relied on the availability of high-coverage genomes from both Neanderthals and Denisovans. Here we search for evidence of archaic admixture from a worldwide panel of 1,667 individuals using an approach that does not require the presence of an archaic human reference genome. We find no evidence for archaic admixture in the Andaman Islands, as previously claimed, or on the island of Flores, where Homo floresiensis fossils have been found. However, we do find evidence for at least one archaic admixture event in sub-Saharan Africa, with the strongest signal in Khoesan and Pygmy individuals from Southern and Central Africa. The locations of these putative archaic admixture tracts are weighted against functional regions of the genome, consistent with the long-term effects of purifying selection against introgressed genetic material.}, }
@article {pmid31793960, year = {2019}, author = {Buzlukov, AL and Arapova, IY and Baklanova, YV and Medvedeva, NI and Denisova, TA and Savina, AA and Lazoryak, BI and Khaikina, EG and Bardet, M}, title = {Coexistence of three types of sodium motion in double molybdate Na9Sc(MoO4)6: 23Na and 45Sc NMR data and ab initio calculations.}, journal = {Physical chemistry chemical physics : PCCP}, volume = {22}, number = {1}, pages = {144-154}, doi = {10.1039/c9cp05249f}, pmid = {31793960}, issn = {1463-9084}, abstract = {The rechargeable Na-ion batteries attract much attention as an alternative to the widely used but expensive Li-ion batteries. The search for materials with high sodium diffusion is important for the development of solid state electrolytes. We present the results of experimental and ab initio studies of the Na-ion diffusion mechanism in Na9Sc(MoO4)6. The ion conductivity reaches the value of 3.6 × 10-2 S cm-1 at T ∼ 850 K. The 23Na and 45Sc NMR data reveal the coexistence of three different types of Na-ion motion in the temperature range from 300 to 750 K. They are activated at different temperatures and are characterized by substantially different dynamics parameters. These features are confirmed by ab initio calculations of activation barriers for sodium diffusion along various paths.}, }
@article {pmid31793531, year = {2019}, author = {Bulynko, SA and Denisova, OA and Kovalets, ES and Kislyakov, AN and Polunin, MM and Soldatsky, YL and Bogomilsky, MR}, title = {[Two cases of bilateral lymphangiomatous palatine tonsil polyps in children].}, journal = {Vestnik otorinolaringologii}, volume = {84}, number = {5}, pages = {73-75}, doi = {10.17116/otorino20198405173}, pmid = {31793531}, issn = {0042-4668}, mesh = {Child ; Humans ; Palatine Tonsil ; *Polyps ; Tonsillar Neoplasms ; }, abstract = {Lymphangiomatous polyps of palatine tonsils are a rare condition, which is diagnosed with patomorphological study. About cases in total are reported in the literature, mostly with one-sided lesions. We managed to find only two reports of lymphangiomatous polyps of palatine tonsils in the available literature. The patients were children in both cases. We report our two cases of children with lymphangiomatous polyps of palatine tonsils.}, }
@article {pmid31784916, year = {2019}, author = {Evseeva, NV and Tkachenko, OV and Denisova, AY and Burygin, GL and Veselov, DS and Matora, LY and Shchyogolev, SY}, title = {Functioning of plant-bacterial associations under osmotic stress in vitro.}, journal = {World journal of microbiology &amp; biotechnology}, volume = {35}, number = {12}, pages = {195}, pmid = {31784916}, issn = {1573-0972}, support = {19-016-00116//Russian Foundation for basic research/ ; }, mesh = {Azospirillum brasilense/physiology ; Chlorophyll A ; Colony Count, Microbial ; Droughts ; Host Microbial Interactions/*physiology ; Malonates ; Ochrobactrum/physiology ; *Osmotic Pressure ; *Plant Development ; Plant Leaves ; Plant Roots/microbiology ; Plant Shoots ; Rhizosphere ; Solanum tuberosum/*microbiology ; Stress, Physiological/*physiology ; }, abstract = {The search for effective plant-growth-promoting strains of rhizospheric bacteria that would ensure the resistance of plant-microbial associations to environmental stressors is essential for the design of environmentally friendly agrobiotechnologies. We investigated the interaction of potato (cv. Nevsky) microplants with the plant-growth-promoting bacteria Azospirillum brasilense Sp245 and Ochrobactrum cytisi IPA7.2 under osmotic stress in vitro. The bacteria improved the physiological and biochemical variables of the microplants, significantly increasing shoot length and root number (1.3-fold, on average). Inoculation also led a more effective recovery of the plants after stress. During repair, inoculation contributed to a decreased leaf content of malonic dialdehyde. With A. brasilense Sp245, the decrease was 1.75-fold; with O. cytisi IPA7.2, it was 1.4-fold. During repair, the shoot length, node number, and root number of the inoculated plants were greater than the control values by an average of 1.3-fold with A. brasilense Sp245 and by an average of 1.6-fold with O. cytisi IPA7.2. O. cytisi IPA7.2, previously isolated from the potato rhizosphere, protected the physiological and biochemical processes in the plants under stress and repair better than did A. brasilense Sp245. Specifically, root weight increased fivefold during repair, as compared to the noninoculated plants, while chlorophyll a content remained at the level found in the nonstressed controls. The results indicate that these bacteria can be used as components of biofertilizers. A. brasilense Sp245 has favorable prospects for use in temperate latitudes, whereas O. cytisi IPA7.2 can be successfully used in saline and drought-stressed environments.}, }
@article {pmid31783664, year = {2019}, author = {Bazhan, N and Jakovleva, T and Feofanova, N and Denisova, E and Dubinina, A and Sitnikova, N and Makarova, E}, title = {Sex Differences in Liver, Adipose Tissue, and Muscle Transcriptional Response to Fasting and Refeeding in Mice.}, journal = {Cells}, volume = {8}, number = {12}, pages = {}, pmid = {31783664}, issn = {2073-4409}, mesh = {Adipose Tissue/*metabolism ; Animals ; Carnitine O-Palmitoyltransferase/metabolism ; Fasting/*metabolism ; Fatty Acid Synthase, Type I/metabolism ; Fatty Acids/metabolism ; Female ; Fibroblast Growth Factors/metabolism ; Liver/*metabolism ; Male ; Mice ; Mice, Inbred C57BL ; PPAR alpha/metabolism ; Quadriceps Muscle/*metabolism ; Refeeding Syndrome/*metabolism ; *Sex Characteristics ; Uncoupling Protein 3/metabolism ; }, abstract = {Fasting is often used for obesity correction but the "refeeding syndrome" limits its efficiency, and molecular mechanisms underlying metabolic response to different food availability are under investigation. Sex was shown to affect hormonal and metabolic reactions to fasting/refeeding. The aim of this study was to evaluate hormonal and transcriptional responses to fasting and refeeding in male and female C57Bl/6J mice. Sex asymmetry was observed both at the hormonal and transcriptional levels. Fasting (24 h) induced increase in hepatic Fgf21 gene expression, which was associated with elevation of plasma FGF21 and adiponectin levels, and the upregulation of expression of hepatic (Pparα, Cpt1α) and muscle (Cpt1β, Ucp3) genes involved in fatty acid oxidation. These changes were more pronounced in females. Refeeding (6 h) evoked hyperinsulinemia and increased hepatic expression of gene related to lipogenesis (Fasn) only in males and hyperleptinemia and increase in Fgf21 gene expression in muscles and adipose tissues only in females. The results suggest that in mice, one of the molecular mechanisms underlying sex asymmetry in hepatic Pparα, Cpt1α, muscle Cpt1β, and Ucp3 expression during fasting is hepatic Fgf21 expression, and the reason for sex asymmetry in hepatic Fasn expression during refeeding is male-specific hyperinsulinemia.}, }
@article {pmid31724235, year = {2019}, author = {Eremin, DB and Denisova, EA and Yu Kostyukovich, A and Martens, J and Berden, G and Oomens, J and Khrustalev, VN and Chernyshev, VM and Ananikov, VP}, title = {Ionic Pd/NHC Catalytic System Enables Recoverable Homogeneous Catalysis: Mechanistic Study and Application in the Mizoroki-Heck Reaction.}, journal = {Chemistry (Weinheim an der Bergstrasse, Germany)}, volume = {}, number = {}, pages = {}, doi = {10.1002/chem.201904825}, pmid = {31724235}, issn = {1521-3765}, abstract = {Invited for the cover of this issue is Valentine P. Ananikkov and co-workers. The image depicts the dynamic behaviour of a Pd/NHC catalytic system with easy transition from molecular to ionic complex. Read the full text of the article at 10.1002/chem.201903221.}, }
@article {pmid31703240, year = {2020}, author = {Denisova, EI and Kozhevnikova, VV and Bazhan, NM and Makarova, EN}, title = {Sex-specific effects of leptin administration to pregnant mice on the placentae and the metabolic phenotypes of offspring.}, journal = {FEBS open bio}, volume = {10}, number = {1}, pages = {96-106}, pmid = {31703240}, issn = {2211-5463}, mesh = {Animals ; Female ; Fetus/*drug effects/*metabolism ; Hyperglycemia/prevention &amp; control ; Leptin/administration &amp; dosage/*pharmacology ; Male ; Mice ; Mice, Inbred C57BL ; Obesity/drug therapy ; Phenotype ; Placenta/*drug effects/*metabolism ; Pregnancy ; Recombinant Proteins/administration &amp; dosage/metabolism ; *Sex Characteristics ; }, abstract = {Obesity during pregnancy has been shown to increase the risk of metabolic diseases in the offspring. However, the factors within the maternal milieu which affect offspring phenotypes and the underlying mechanisms remain unknown. The adipocyte hormone leptin plays a key role in regulating energy homeostasis and is known to participate in sex-specific developmental programming. To examine the action of leptin on fetal growth, placental gene expression and postnatal offspring metabolism, we injected C57BL mice with leptin or saline on gestational day 12 and then measured body weights (BWs) of offspring fed on a standard or obesogenic diet, as well as mRNA expression levels of insulin-like growth factors and glucose and amino acid transporters. Male and female offspring born to leptin-treated mothers exhibited growth retardation before and a growth surge after weaning. Mature male offspring, but not female offspring, exhibited increased BWs on a standard diet. Leptin administration prevented the development of hyperglycaemia in the obese offspring of both sexes. The placentas of the male and female foetuses differed in size and gene expression, and leptin injection decreased the fetal weights of both sexes, the placental weights of the male foetuses and placental gene expression of the GLUT1 glucose transporter in female foetuses. The data suggest that mid-pregnancy is an ontogenetic window for the sex-specific programming effects of leptin, and these effects may be exerted via fetal sex-specific placental responses to leptin administration.}, }
@article {pmid31702050, year = {2020}, author = {Gokcumen, O}, title = {Archaic hominin introgression into modern human genomes.}, journal = {American journal of physical anthropology}, volume = {171 Suppl 70}, number = {}, pages = {60-73}, doi = {10.1002/ajpa.23951}, pmid = {31702050}, issn = {1096-8644}, support = {1714867//United States National Science Foundation/International ; }, mesh = {Animals ; DNA/genetics ; *Gene Flow ; *Genetic Introgression ; *Genome, Human ; Hominidae/*genetics ; Humans ; Neanderthals/genetics ; }, abstract = {Ancient genomes from multiple Neanderthal and the Denisovan individuals, along with DNA sequence data from diverse contemporary human populations strongly support the prevalence of gene flow among different hominins. Recent studies now provide evidence for multiple gene flow events that leave genetic signatures in extant and ancient human populations. These events include older gene flow from an unknown hominin in Africa predating out-of-Africa migrations, and in the last 50,000-100,000 years, multiple gene flow events from Neanderthals into ancestral Eurasian human populations, and at least three distinct introgression events from a lineage close to Denisovans into ancestors of extant Southeast Asian and Oceanic populations. Some of these introgression events may have happened as late as 20,000 years before present and reshaped the way in which we think about human evolution. In this review, I aim to answer anthropologically relevant questions with regard to recent research on ancient hominin introgression in the human lineage. How have genomic data from archaic hominins changed our view of human evolution? Is there any doubt about whether introgression from ancient hominins to the ancestors of present-day humans occurred? What is the current view of human evolutionary history from the genomics perspective? What is the impact of introgression on human phenotypes?}, }
@article {pmid31686394, year = {2020}, author = {Denisova, O and Chernogoryuk, G and Baranovskaya, N and Rikhvanov, L and Shefer, N and Chernjavskaya, G and Palchikova, I and Kalacheva, T}, title = {Trace Elements in the Lung Tissue Affected by Sarcoidosis.}, journal = {Biological trace element research}, volume = {196}, number = {1}, pages = {66-73}, doi = {10.1007/s12011-019-01915-z}, pmid = {31686394}, issn = {1559-0720}, mesh = {Adult ; Female ; Humans ; Inflammation/*chemically induced/pathology ; Lung/*chemistry/pathology ; Male ; Middle Aged ; Neutron Activation Analysis ; Sarcoidosis/*chemically induced/pathology ; Trace Elements/*analysis ; }, abstract = {In the lungs of 76 patients with verified sarcoidosis, 28 chemical elements were identified with neutron activation analysis. High levels of Ca, Fe, Cr, Co, Cs, Eu, Lu, Th, Hf, Au, and U and low level of Na compared to the control samples were determined in sarcoidosis. There were no significant differences in the content of Zn, Rb, La, Sm, Sr, Nd, As, Br, Ag, Tb, Sc, Ta, Sb, Ba, and Yb. Spearman correlation analysis shows multiple positive associations, with the maximum being in pairs as follows: Fe-Cr, Eu-La, Ce-Lu, Hf-Cr, Sc-Zn, Fe-Hf, Ce-Co, and Sb-Cr. These studies support the hypothesis that sarcoidosis is a response of the organism in the form of granulomatous inflammation when exposed to heavy metals and rare earth elements in the environment. We assume that the role of calcium and iron is to separate granulomas from the tissues of the body.}, }
@article {pmid31591491, year = {2019}, author = {Colbran, LL and Gamazon, ER and Zhou, D and Evans, P and Cox, NJ and Capra, JA}, title = {Inferred divergent gene regulation in archaic hominins reveals potential phenotypic differences.}, journal = {Nature ecology &amp; evolution}, volume = {3}, number = {11}, pages = {1598-1606}, pmid = {31591491}, issn = {2397-334X}, support = {R01 MH113362/MH/NIMH NIH HHS/United States ; R35 GM127087/GM/NIGMS NIH HHS/United States ; R35 HG010718/HG/NHGRI NIH HHS/United States ; }, mesh = {Animals ; Female ; Genome, Human ; Haplotypes ; *Hominidae ; Humans ; *Neanderthals ; Phenotype ; }, abstract = {Sequencing DNA derived from archaic bones has enabled genetic comparison of Neanderthals and anatomically modern humans (AMHs), and revealed that they interbred. However, interpreting what genetic differences imply about their phenotypic differences remains challenging. Here, we introduce an approach for identifying divergent gene regulation between archaic hominins, such as Neanderthals, and AMH sequences, and find 766 genes that are likely to have been divergently regulated (DR) by Neanderthal haplotypes that do not remain in AMHs. DR genes include many involved in phenotypes known to differ between Neanderthals and AMHs, such as the structure of the rib cage and supraorbital ridge development. They are also enriched for genes associated with spontaneous abortion, polycystic ovary syndrome, myocardial infarction and melanoma. Phenotypes associated with modern human variation in these genes' regulation in ~23,000 biobank patients further support their involvement in immune and cardiovascular phenotypes. Comparing DR genes between two Neanderthals and a Denisovan revealed divergence in the immune system and in genes associated with skeletal and dental morphology that are consistent with the archaeological record. These results establish differences in gene regulatory architecture between AMHs and archaic hominins, and provide an avenue for exploring phenotypic differences between archaic groups from genomic information alone.}, }
@article {pmid31564255, year = {2019}, author = {Shchenkov, SV and Denisova, SA and Kremnev, GA and Dobrovolskij, AA}, title = {Five new morphological types of virgulate and microcotylous xiphidiocercariae based on morphological and molecular phylogenetic analyses.}, journal = {Journal of helminthology}, volume = {94}, number = {}, pages = {e94}, doi = {10.1017/S0022149X19000853}, pmid = {31564255}, issn = {1475-2697}, mesh = {Animals ; Biological Evolution ; Cercaria/classification/genetics/*growth &amp; development/*isolation &amp; purification ; Female ; Male ; *Phylogeny ; }, abstract = {The phylogenetic position of most xiphidiocercariae from subgroups Cercariae virgulae and Cercariae microcotylae remains unknown or unclear, even at the family level. In this paper, we studied the morphology and molecular phylogeny of 15 microcotylous and virgulate cercariae (11 new and four previously described ones). Based on morphological and molecular data, we suggested five distinct morphological types of xiphidiocercariae, which are a practical alternative to Cercariae virgulae and Cercariae microcotylae subgroups. Four of these types correspond to actual digenean taxa (Microphallidae, Lecithodendriidae, Pleurogenidae and Prosthogonimidae), while the fifth is represented by Cercaria nigrospora Wergun, 1957, which we classified on the basis of molecular data for the first time. We reassessed the relative importance of morphological characters used for the classification of virgulate and microcotylous cercariae, and discussed the main evolutionary trends within xiphidiocercariae. Now stylet cercariae can be reliably placed into several sub-taxa of Microphalloidea on the basis of their morphological features.}, }
@article {pmid31560950, year = {2019}, author = {Mata, X and Renaud, G and Mollereau, C}, title = {The repertoire of family A-peptide GPCRs in archaic hominins.}, journal = {Peptides}, volume = {122}, number = {}, pages = {170154}, doi = {10.1016/j.peptides.2019.170154}, pmid = {31560950}, issn = {1873-5169}, mesh = {Animals ; Diabetic Nephropathies/*genetics/pathology ; *Evolution, Molecular ; Genome, Human/genetics ; Haplotypes/genetics ; Hominidae/genetics ; Humans ; Neanderthals/genetics ; Obesity/*genetics/pathology ; Peptides/genetics ; Platelet Aggregation/genetics ; Receptors, G-Protein-Coupled/*genetics ; Risk Factors ; }, abstract = {Given the importance of G-protein coupled receptors in the regulation of many physiological functions, deciphering the relationships between genotype and phenotype in past and present hominin GPCRs is of main interest to understand the evolutionary process that contributed to the present-day variability in human traits and health. Here, we carefully examined the publicly available genomic and protein sequence databases of the archaic hominins (Neanderthal and Denisova) to draw up the catalog of coding variations in GPCRs for peptide ligands, in comparison with living humans. We then searched in the literature the functional changes, phenotypes and risk of disease possibly associated with the detected variants. Our survey suggests that Neanderthal and Denisovan hominins were likely prone to lower risk of obesity, to enhanced platelet aggregation in response to thrombin, to better response to infection, to less anxiety and aggressiveness and to favorable sociability. While some archaic variants were likely advantageous in the past, they might be responsible for maladaptive disorders today in the context of modern life and/or specific regional distribution. For example, an archaic haplotype in the neuromedin receptor 2 is susceptible to confer risk of diabetic nephropathy in type 1 diabetes in present-day Europeans. Paying attention to the pharmacological properties of some of the archaic variants described in this study may be helpful to understand the variability of therapeutic efficacy between individuals or ethnic groups.}, }
@article {pmid31558742, year = {2019}, author = {Morley, MW and Goldberg, P and Uliyanov, VA and Kozlikin, MB and Shunkov, MV and Derevianko, AP and Jacobs, Z and Roberts, RG}, title = {Hominin and animal activities in the microstratigraphic record from Denisova Cave (Altai Mountains, Russia).}, journal = {Scientific reports}, volume = {9}, number = {1}, pages = {13785}, pmid = {31558742}, issn = {2045-2322}, mesh = {Animals ; Archaeology ; *Caves ; Cold Climate ; DNA, Ancient/isolation &amp; purification ; *Fossils ; History, Ancient ; *Hominidae/genetics ; Humans ; Neanderthals/genetics ; Paleontology ; Siberia ; }, abstract = {Denisova Cave in southern Siberia uniquely contains evidence of occupation by a recently discovered group of archaic hominins, the Denisovans, starting from the middle of the Middle Pleistocene. Artefacts, ancient DNA and a range of animal and plant remains have been recovered from the sedimentary deposits, along with a few fragmentary fossils of Denisovans, Neanderthals and a first-generation Neanderthal-Denisovan offspring. The deposits also contain microscopic traces of hominin and animal activities that can provide insights into the use of the cave over the last 300,000 years. Here we report the results of a micromorphological study of intact sediment blocks collected from the Pleistocene deposits in the Main and East Chambers of Denisova Cave. The presence of charcoal attests to the use of fire by hominins, but other evidence of their activities preserved in the microstratigraphic record are few. The ubiquitous occurrence of coprolites, which we attribute primarily to hyenas, indicates that the site was visited for much of its depositional history by cave-dwelling carnivores. Microscopic traces of post-depositional diagenesis, bioturbation and incipient cryoturbation are observed in only a few regions of the deposit examined here. Micromorphology can help identify areas of sedimentary deposit that are most conducive to ancient DNA preservation and could be usefully integrated with DNA analyses of sediments at archaeological sites to illuminate features of their human and environmental history that are invisible to the naked eye.}, }
@article {pmid31539495, year = {2019}, author = {Gokhman, D and Mishol, N and de Manuel, M and de Juan, D and Shuqrun, J and Meshorer, E and Marques-Bonet, T and Rak, Y and Carmel, L}, title = {Reconstructing Denisovan Anatomy Using DNA Methylation Maps.}, journal = {Cell}, volume = {179}, number = {1}, pages = {180-192.e10}, doi = {10.1016/j.cell.2019.08.035}, pmid = {31539495}, issn = {1097-4172}, mesh = {Animals ; Base Sequence ; DNA Methylation/*genetics ; Databases, Genetic ; Extinction, Biological ; Fossils ; Genome, Human/genetics ; Humans ; Neanderthals/*anatomy &amp; histology/*genetics ; Pan troglodytes/*anatomy &amp; histology/*genetics ; *Phenotype ; Polymorphism, Single Nucleotide/genetics ; Skeleton ; Skull ; }, abstract = {Denisovans are an extinct group of humans whose morphology remains unknown. Here, we present a method for reconstructing skeletal morphology using DNA methylation patterns. Our method is based on linking unidirectional methylation changes to loss-of-function phenotypes. We tested performance by reconstructing Neanderthal and chimpanzee skeletal morphologies and obtained >85% precision in identifying divergent traits. We then applied this method to the Denisovan and offer a putative morphological profile. We suggest that Denisovans likely shared with Neanderthals traits such as an elongated face and a wide pelvis. We also identify Denisovan-derived changes, such as an increased dental arch and lateral cranial expansion. Our predictions match the only morphologically informative Denisovan bone to date, as well as the Xuchang skull, which was suggested by some to be a Denisovan. We conclude that DNA methylation can be used to reconstruct anatomical features, including some that do not survive in the fossil record.}, }
@article {pmid31534238, year = {2019}, author = {Zammit, NW and Siggs, OM and Gray, PE and Horikawa, K and Langley, DB and Walters, SN and Daley, SR and Loetsch, C and Warren, J and Yap, JY and Cultrone, D and Russell, A and Malle, EK and Villanueva, JE and Cowley, MJ and Gayevskiy, V and Dinger, ME and Brink, R and Zahra, D and Chaudhri, G and Karupiah, G and Whittle, B and Roots, C and Bertram, E and Yamada, M and Jeelall, Y and Enders, A and Clifton, BE and Mabbitt, PD and Jackson, CJ and Watson, SR and Jenne, CN and Lanier, LL and Wiltshire, T and Spitzer, MH and Nolan, GP and Schmitz, F and Aderem, A and Porebski, BT and Buckle, AM and Abbott, DW and Ziegler, JB and Craig, ME and Benitez-Aguirre, P and Teo, J and Tangye, SG and King, C and Wong, M and Cox, MP and Phung, W and Tang, J and Sandoval, W and Wertz, IE and Christ, D and Goodnow, CC and Grey, ST}, title = {Denisovan, modern human and mouse TNFAIP3 alleles tune A20 phosphorylation and immunity.}, journal = {Nature immunology}, volume = {20}, number = {10}, pages = {1299-1310}, doi = {10.1038/s41590-019-0492-0}, pmid = {31534238}, issn = {1529-2916}, support = {R01 AI052127/AI/NIAID NIH HHS/United States ; U19 AI100627/AI/NIAID NIH HHS/United States ; U54 AI054523/AI/NIAID NIH HHS/United States ; }, mesh = {Alleles ; Animals ; Extinction, Biological ; Humans ; Immunity ; Inflammation ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Mutation, Missense/genetics ; Phosphorylation ; Poxviridae/*physiology ; Poxviridae Infections/*immunology ; Protein Domains/*genetics ; Tumor Necrosis Factor alpha-Induced Protein 3/*genetics ; }, abstract = {Resisting and tolerating microbes are alternative strategies to survive infection, but little is known about the evolutionary mechanisms controlling this balance. Here genomic analyses of anatomically modern humans, extinct Denisovan hominins and mice revealed a TNFAIP3 allelic series with alterations in the encoded immune response inhibitor A20. Each TNFAIP3 allele encoded substitutions at non-catalytic residues of the ubiquitin protease OTU domain that diminished IκB kinase-dependent phosphorylation and activation of A20. Two TNFAIP3 alleles encoding A20 proteins with partial phosphorylation deficits seemed to be beneficial by increasing immunity without causing spontaneous inflammatory disease: A20 T108A;I207L, originating in Denisovans and introgressed in modern humans throughout Oceania, and A20 I325N, from an N-ethyl-N-nitrosourea (ENU)-mutagenized mouse strain. By contrast, a rare human TNFAIP3 allele encoding an A20 protein with 95% loss of phosphorylation, C243Y, caused spontaneous inflammatory disease in humans and mice. Analysis of the partial-phosphorylation A20 I325N allele in mice revealed diminished tolerance of bacterial lipopolysaccharide and poxvirus inoculation as tradeoffs for enhanced immunity.}, }
@article {pmid31517046, year = {2019}, author = {Bennett, EA and Crevecoeur, I and Viola, B and Derevianko, AP and Shunkov, MV and Grange, T and Maureille, B and Geigl, EM}, title = {Morphology of the Denisovan phalanx closer to modern humans than to Neanderthals.}, journal = {Science advances}, volume = {5}, number = {9}, pages = {eaaw3950}, pmid = {31517046}, issn = {2375-2548}, mesh = {Animals ; Finger Phalanges/*anatomy &amp; histology ; *Genome, Human ; Humans ; Molar/*anatomy &amp; histology ; *Neanderthals/anatomy &amp; histology/genetics ; Species Specificity ; }, abstract = {A fully sequenced high-quality genome has revealed in 2010 the existence of a human population in Asia, the Denisovans, related to and contemporaneous with Neanderthals. Only five skeletal remains are known from Denisovans, mostly molars; the proximal fragment of a fifth finger phalanx used to generate the genome, however, was too incomplete to yield useful morphological information. Here, we demonstrate through ancient DNA analysis that a distal fragment of a fifth finger phalanx from the Denisova Cave is the larger, missing part of this phalanx. Our morphometric analysis shows that its dimensions and shape are within the variability of Homo sapiens and distinct from the Neanderthal fifth finger phalanges. Thus, unlike Denisovan molars, which display archaic characteristics not found in modern humans, the only morphologically informative Denisovan postcranial bone identified to date is suggested here to be plesiomorphic and shared between Denisovans and modern humans.}, }
@article {pmid31506618, year = {2019}, author = {Callaway, E}, title = {Lost Denisovan bone reveals surprisingly human-like finger.}, journal = {Nature}, volume = {573}, number = {7773}, pages = {175-176}, doi = {10.1038/d41586-019-02647-9}, pmid = {31506618}, issn = {1476-4687}, mesh = {Animals ; Archaeology ; Fingers ; *Hominidae ; Humans ; *Neanderthals ; }, }
@article {pmid31475413, year = {2020}, author = {Garduño-Alanís, A and Malyutina, S and Pajak, A and Stepaniak, U and Kubinova, R and Denisova, D and Pikhart, H and Peasey, A and Bobak, M and Stefler, D}, title = {Association between soft drink, fruit juice consumption and obesity in Eastern Europe: cross-sectional and longitudinal analysis of the HAPIEE study.}, journal = {Journal of human nutrition and dietetics : the official journal of the British Dietetic Association}, volume = {33}, number = {1}, pages = {66-77}, doi = {10.1111/jhn.12696}, pmid = {31475413}, issn = {1365-277X}, support = {R01 AG023522/AG/NIA NIH HHS/United States ; WT064947/WT_/Wellcome Trust/United Kingdom ; WT081081/WT_/Wellcome Trust/United Kingdom ; }, mesh = {Aged ; Body Mass Index ; Carbonated Beverages/adverse effects/*statistics &amp; numerical data ; Cross-Sectional Studies ; Czech Republic/epidemiology ; Diet Surveys ; Drinking Behavior ; Female ; Follow-Up Studies ; Fruit and Vegetable Juices/adverse effects/*statistics &amp; numerical data ; Humans ; Longitudinal Studies ; Male ; Middle Aged ; Obesity/*epidemiology/etiology ; Poland/epidemiology ; Prospective Studies ; Russia/epidemiology ; }, abstract = {BACKGROUND: Fruit juice and soft drink consumption have been shown to be related to obesity. However, this relationship has not been explored in Eastern Europe. The present study aimed to assess the cross-sectional and longitudinal relationships between fruit juice, soft drink consumption and body mass index (BMI) in Eastern European cohorts.<br><br>METHODS: Data from the Health, Alcohol and Psychosocial factors in Eastern Europe population-based prospective cohort study, based in Russia, Poland and the Czech Republic, were used. Intakes of sugar-sweetened beverage (SSB), artificially-sweetened beverage (ASB) and fruit juice were estimated from a food frequency questionnaire. Participant BMI values were assessed at baseline (n = 26 634) and after a 3-year follow-up (data available only for Russia, n = 5205).<br><br>RESULTS: Soft drink consumption was generally low, particularly in Russia. Compared to never drinkers of SSB, participants who drank SSB every day had a significantly higher BMI in the Czech [β-coefficient = 0.28; 95% confidence interval (CI) = 0.02-0.54], Russian (β-coefficient = 1.38; 95% CI = 0.62-2.15) and Polish (β-coefficient = 0.83; 95% CI = 0.29-1.37) cohorts. Occasional or daily ASB consumption was also positively associated with BMI in all three cohorts. Results for daily fruit juice intake were inconsistent, with a positive association amongst Russians (β-coefficient = 0.75; 95% CI = 0.28-1.21) but a negative trend in the Czech Republic (β-coefficient = -0.42; 95% CI = -0.86 to 0.02). Russians participants who drank SSB or ASB had an increased BMI after follow-up.<br><br>CONCLUSIONS: Our findings support previous studies suggesting that soft drink consumption (including SSBs and ASBs) is positively related to BMI, whereas our results for fruit juice were less consistent. Policies regarding these beverages should be considered in Eastern Europe to lower the risk of obesity.}, }
@article {pmid33951887, year = {2019}, author = {Caldararo, N}, title = {Probability, Populations, Phylogenetics, and Hominin Speciation.}, journal = {Human biology}, volume = {90}, number = {2}, pages = {129-155}, doi = {10.13110/humanbiology.90.2.04}, pmid = {33951887}, issn = {1534-6617}, abstract = {A number of recent articles have appeared on the hominin Denisova fossil remains. Many of them focus on attempts to produce DNA sequences from the extracted samples. Often these project mitochondrial DNA (mtDNA) sequences from the fossils of a number of Neandertals and the Denisovans in an attempt to understand the evolution of Middle Pleistocene human ancestors. These articles introduce a number of problems in the interpretation of speciation in hominins. One concerns the degradation of the ancient DNA and its interpretation as authentic genetic information. Another problem concerns the ideas of "species" versus "population" and the use of these ideas in building evolutionary diagrams to indicate ancestry and extinction. A third issue concerns the theory of haplotypes in the mtDNA. Given the severe constraints on mutations in the mtDNA genome to maintain functionality and the purifying processes to reduce such mutations in the ovaries, putative geographic and historical variations seem contradictory. Local diversity and variations in supposed "macrohaplotypes" are explained as back migrations or back mutations, which dilutes the robust nature of the theory. A central issue involves what human variation means, how much population variation there has been in the past, and whether this variation distinguishes hominid speciation or is simply a process of anagenesis. This brings up the question of how much can be interpreted from the analysis of DNA. Some businesses today claim to be able to use DNA analysis to discover past ethnic identities, and a new niche in restaurants is producing "DNA" menus. Perhaps some caution is in order.}, }
@article {pmid31461192, year = {2019}, author = {Eremin, DB and Denisova, EA and Yu Kostyukovich, A and Martens, J and Berden, G and Oomens, J and Khrustalev, VN and Chernyshev, VM and Ananikov, VP}, title = {Ionic Pd/NHC Catalytic System Enables Recoverable Homogeneous Catalysis: Mechanistic Study and Application in the Mizoroki-Heck Reaction.}, journal = {Chemistry (Weinheim an der Bergstrasse, Germany)}, volume = {}, number = {}, pages = {}, doi = {10.1002/chem.201903221}, pmid = {31461192}, issn = {1521-3765}, support = {19-13-00460//Russian Science Foundation/ ; }, abstract = {N-Heterocyclic carbene (NHC) ligands are ubiquitously utilized in catalysis. A common catalyst design model assumes strong M-NHC binding in this metal-ligand framework. In contrast to this common assumption, we demonstrate here that lability and controlled cleavage of the M-NHC bond (rather than its stabilization) could be more important for high-performance catalysis at low catalyst concentrations. The present study reveals a dynamic stabilization mechanism with labile metal-NHC binding and [PdX3 ]- [NHC-R]+ ion pair formation. Access to reactive anionic palladium intermediates formed by dissociation of the NHC ligands and plausible stabilization of the molecular catalyst in solution by interaction with the [NHC-R]+ azolium ion is of particular importance for an efficient and recyclable catalyst. These ionic Pd/NHC complexes allowed for the first time the recycling of the complex in a well-defined form with isolation at each cycle. Computational investigation of the reaction mechanism confirms a facile formation of NHC-free anionic Pd in polar media through either Ph-NHC coupling or reversible H-NHC coupling. The present study formulates novel ideas for M/NHC catalyst design.}, }
@article {pmid31448903, year = {2019}, author = {Denisova, EA and Eremin, DB and Gordeev, EG and Tsedilin, AM and Ananikov, VP}, title = {Addressing Reversibility of R-NHC Coupling on Palladium: Is Nano-to-Molecular Transition Possible for the Pd/NHC System?.}, journal = {Inorganic chemistry}, volume = {58}, number = {18}, pages = {12218-12227}, doi = {10.1021/acs.inorgchem.9b01630}, pmid = {31448903}, issn = {1520-510X}, abstract = {It has recently been shown that palladium-catalyzed reactions with N-heterocyclic carbene (NHC) ligands involve R-NHC coupling accompanied by transformation of the molecular catalytic system into the nanoscale catalytic system. An important question appeared in this regard is whether such a change in the catalytic system is irreversible. More specifically, is the reverse nano-to-molecular transformation possible? In view of the paramount significance of this question to the area of catalyst design, we studied the capability of 2-substituted azolium salts to undergo the breakage of C-C bond and exchange substituents on the carbene carbon with corresponding aryl halides in the presence of Pd nanoparticles. The study provides important experimental evidence of possibility of the reversible R-NHC coupling. The observed behavior indicates that the nanosized metal species are capable of reverse transition to molecular species. Such an option, known for phosphine ligands, was previously unexplored for NHC ligands. The present study for the first time demonstrates bidirectional dynamic transitions between the molecular and nanostructured states in Pd/NHC systems. As a unique feature, surprisingly small activation barriers (<18 kcal/mol) and noticeable thermodynamic driving force (-5 to -7 kcal/mol) were calculated for C-C bond oxidative addition to Pd(0) centers in the studied system. The first example of NHC-mediated Pd leaching from metal nanoparticles to solution was observed and formation of Pd/NHC complex in solution was detected by ESI-MS.}, }
@article {pmid31386798, year = {2020}, author = {Mikaeeli, S and Susan-Resiga, D and Girard, E and Ben Djoudi Ouadda, A and Day, R and Prost, S and Seidah, NG}, title = {Functional analysis of natural PCSK9 mutants in modern and archaic humans.}, journal = {The FEBS journal}, volume = {287}, number = {3}, pages = {515-528}, doi = {10.1111/febs.15036}, pmid = {31386798}, issn = {1742-4658}, support = {148363//CIHR/Canada ; }, mesh = {Animals ; Binding Sites ; DNA Methylation ; Humans ; *Loss of Function Mutation ; Neanderthals/*genetics ; Proprotein Convertase 9/chemistry/*genetics/metabolism ; Protein Binding ; Receptors, LDL/metabolism ; }, abstract = {PCSK9 is the last member of the proprotein convertases (PCs) family and its gene is mutated in ~ 2% to 3% of individuals with familial hypercholesterolemia (FH). This protein enhances the degradation of the low-density lipoprotein receptor (LDLR) and hence increases the levels of circulating LDL-cholesterol (LDLc). Studies of the underlying mechanism(s) regulating the activity of different mutations in the PCSK9 gene are ongoing as they enhance our understanding of the biology and clinical relevance of PCSK9 and its partners. In an attempt to unravel the regulation of PCSK9 transcription and possibly identify mutation 'hot spot' regions with alterations in CpG methylation, we present for the first time the complete methylome profile of the PCSK9 gene in modern and archaic humanoids. Our data showed that the genomes of modern humans and archaic PCSK9 exhibit a similar methylation pattern. Next, we defined the mechanistic consequences of three PCSK9 natural mutations (PCSK9-R96L, -R105W, and -P174S) and one archaic Denisovan mutation (PCSK9-H449L) using various complementary cellular and in vitro binding assays. Our results showed that the PCSK9-H449L is a loss-of-function (LOF) mutation, likely due to its lower binding affinity to the LDLR. Similarly, PCSK9-R96L and -R105W are LOF mutations, even though they have been identified in FH patients. The PCSK9-R105W mutation leads to a significantly lower autocatalytic processing of proPCSK9. PCSK9-P174S resulted in a LOF in both extracellular and intracellular pathways. In conclusion, our extensive analyses revealed that all studied mutations result in PCSK9 LOF, via various mechanisms, leading to lower levels of LDLc.}, }
@article {pmid31372716, year = {2019}, author = {Derenko, M and Denisova, G and Malyarchuk, B and Hovhannisyan, A and Khachatryan, Z and Hrechdakian, P and Litvinov, A and Yepiskoposyan, L}, title = {Insights into matrilineal genetic structure, differentiation and ancestry of Armenians based on complete mitogenome data.}, journal = {Molecular genetics and genomics : MGG}, volume = {294}, number = {6}, pages = {1547-1559}, pmid = {31372716}, issn = {1617-4623}, support = {The state assignment theme for the IBPN FEB RAS (0290-2018-0024)//the Ministry of Science and Higher Education of Russia/ ; 18T-1F186//State Committee of Science/ ; NT-18/TB//State Committee of Science/ ; hubio-4562//Armenian National Science and Education Fund/ ; }, mesh = {Armenia ; Asia, Central ; Asia, Western ; DNA, Mitochondrial/chemistry ; Europe ; Genetic Variation ; *Genome, Mitochondrial ; Haplotypes ; Humans ; Phylogeny ; Phylogeography ; }, abstract = {Distinctive peculiarities of Armenians such as their millennia-long genetic isolation and strong national identity attract a keen interest while studying the demographic history of the West Asia. Here, to examine their fine-scale matrilineal genetic structure, ancestry and relationships with neighboring populations, we analyzed 536 complete mitogenomes (141 of which are novel) from 8 geographically different Armenian populations, covering the whole stretch of historical Armenia. The observed patterns highlight a remarkable degree of matrilineal genetic heterogeneity and weak population structuring of Armenians. Moreover, our phylogeographic analysis reveals common ancestries for some mtDNA lineages shared by West Asians, Transcaucasians, Europeans, Central Asians and Armenians. About third of the mtDNA subhaplogroups found in Armenian gene pool might be considered as Armenian-specific, as these are virtually absent elsewhere in Europe, West Asia and Transcaucasia. Coalescence ages of most of these lineages do not exceed 3.1 kya and coincide well with the population size growth started around 1.8-2.8 kya detectable only in the Bayesian Skyline Plots based on the Armenian-specific mtDNA haplotypes.}, }
@article {pmid31285349, year = {2019}, author = {Bailey, SE and Hublin, JJ and Antón, SC}, title = {Rare dental trait provides morphological evidence of archaic introgression in Asian fossil record.}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {116}, number = {30}, pages = {14806-14807}, pmid = {31285349}, issn = {1091-6490}, mesh = {Animals ; Asian Continental Ancestry Group/genetics ; *Evolution, Molecular ; Fossils/*anatomy &amp; histology ; Hominidae/*anatomy &amp; histology/genetics ; Humans ; Mandible/anatomy &amp; histology ; Molar/*anatomy &amp; histology ; }, abstract = {The recently described Denisovan hemimandible from Xiahe, China [F. Chen et al., (2019) Nature 569, 409-412], possesses an unusual dental feature: a 3-rooted lower second molar. A survey of the clinical and bioarchaeological literature demonstrates that the 3-rooted lower molar is rare (less than 3.5% occurrence) in non-Asian Homo sapiens In contrast, its presence in Asian-derived populations can exceed 40% in China and the New World. It has long been thought that the prevalence of 3-rooted lower molars in Asia is a relatively late acquisition occurring well after the origin and dispersal of H. sapiens However, the presence of a 3-rooted lower second molar in this 160,000-y-old fossil hominin suggests greater antiquity for the trait. Importantly, it also provides morphological evidence of a strong link between archaic and recent Asian H. sapiens populations. This link provides compelling evidence that modern Asian lineages acquired the 3-rooted lower molar via introgression from Denisovans.}, }
@article {pmid31278514, year = {2019}, author = {Brzozowska, MM and Havula, E and Allen, RB and Cox, MP}, title = {Genetics, adaptation to environmental changes and archaic admixture in the pathogenesis of diabetes mellitus in Indigenous Australians.}, journal = {Reviews in endocrine &amp; metabolic disorders}, volume = {20}, number = {3}, pages = {321-332}, pmid = {31278514}, issn = {1573-2606}, mesh = {Australia ; Diabetes Mellitus, Type 2/*genetics/*pathology ; Genome-Wide Association Study ; Humans ; Indigenous Peoples ; Obesity/genetics/pathology ; }, abstract = {Indigenous Australians are particularly affected by type 2 diabetes mellitus (T2D) due to both their genetic susceptibility and a range of environmental and lifestyle risk factors. Recent genetic studies link predisposition to some diseases, including T2D, to alleles acquired from archaic hominins, such as Neanderthals and Denisovans, which persist in the genomes of modern humans today. Indo-Pacific human populations, including Indigenous Australians, remain extremely underrepresented in genomic research with a paucity of data examining the impact of Denisovan or Neanderthal lineages on human phenotypes in Oceania. The few genetic studies undertaken emphasize the uniqueness and antiquity of Indigenous Australian genomes, with possibly the largest proportion of Denisovan ancestry of any population in the world. In this review, we focus on the potential contributions of ancient genes/pathways to modern human phenotypes, while also highlighting the evolutionary roles of genetic adaptation to dietary and environmental changes associated with an adopted Western lifestyle. We discuss the role of genetic and epigenetic factors in the pathogenesis of T2D in understudied Indigenous Australians, including the potential impact of archaic gene lineages on this disease. Finally, we propose that greater understanding of the underlying genetic predisposition may contribute to the clinical efficacy of diabetes management in Indigenous Australians. We suggest that improved identification of T2D risk variants in Oceania is needed. Such studies promise to clarify how genetic and phenotypic differences vary between populations and, crucially, provide novel targets for personalised medical therapies in currently marginalized groups.}, }
@article {pmid31233688, year = {2019}, author = {Kobelkova, IV and Martinchik, AN and Keshabyants, EE and Denisova, NN and Peskova, EV and Vybornaya, KV and Sokolov, AI and Lavrinenko, SV and Nikityuk, DB}, title = {[An analysis of the diet of members of the Russian national men's water polo team during the sports training camps].}, journal = {Voprosy pitaniia}, volume = {88}, number = {2}, pages = {50-57}, doi = {10.24411/0042-8833-2019-10017}, pmid = {31233688}, issn = {0042-8833}, support = {theme 0529-2017-0053//Russian Academy of Science/ ; }, mesh = {Adult ; *Athletes ; Dietary Carbohydrates/*administration &amp; dosage ; Dietary Fats/*administration &amp; dosage ; *Energy Intake ; *Energy Metabolism ; Humans ; Male ; *Nutritional Status ; *Water Sports ; }, abstract = {Increasing the adaptive capacity of professional athletes depends on proper nutrition, especially in training and competitions' period. In this regard, it is relevant to study the actual nutrition and assess its compliance with the energy expenditure of athletes. The aim - to study the actual nutrition and energy expenditure of athletes from male water polo national team of the Russian Federation in the competitive period. Material and methods. In March 2018, 15 highly skilled sportsmen-men engaged in water polo were examined; qualification - 11 masters of sports, 4 candidates for the master of sports; Slavic ethnos. The average age was 23.1±0.6 years. The actual nutrition was studied by a 24-hour food record method and by the frequency method. The anthropometric examination was carried out according to a unified method using standard medical scales, a medical height meter and a rubberized measuring tape. Measurement of energy expenditure and heart rate at rest and under load was performed on a bicycle ergometer using an wireless ergospirometer and a chest pulse meter. Results and discussion. The determination of daily energy expenditure in athletes of the men's Russian national water polo team showed that the average value was 4350±129 kcal. А peculiar feature of the diet of water polo players is its high caloric value (5165±539 kcal/day), caused by energy expenditure during physical exertion and additional thermogenesis in conditions of long training in water. Excessive (1.5 times in comparison with the recommended values) consumption of fats, including saturated fatty acids by 1.3 times, added sugar and added salt is a risk factor of cardiovascular diseases, diseases of digestive organs, endocrine system, including type 2 diabetes. Low values of consumption of vegetables and fruits, dairy products, fish products and high levels of sugar and confectionery have been established. Conclusion. The imbalance of diets on two basic nutrients (fats, carbohydrates) has been revealed. The data obtained were the basis for the formation of individual recommendations on nutrition for each athlete, taking into account athletes' metabolic parameters and the level of physical activity. It is necessary to continue studies of anthropometric indices in dynamics for the most adequate assessment of the compliance of actual nutrition with energy consumption, and further correction of the diet in order to improve athletes' performance.}, }
@article {pmid31228375, year = {2019}, author = {Denisova, OA and Livzan, MA and Denisov, AP and Kun, OA}, title = {[Clinical aspect of diagnostics of gastroesophageal reflux disease in elderly patients.].}, journal = {Advances in gerontology = Uspekhi gerontologii}, volume = {32}, number = {1-2}, pages = {108-111}, pmid = {31228375}, issn = {1561-9125}, mesh = {Aged ; Cross-Sectional Studies ; *Gastroesophageal Reflux/complications/diagnosis ; Heartburn ; Humans ; *Quality of Life ; Surveys and Questionnaires ; }, abstract = {Insufficient knowledge age peculiarities of gastroesophageal reflux disease (GERD) in the elderly against the background of its prevalence determine the high demand for studies on the topic. In an open study cohort by cross-sectional analysis was conducted clinical features GERD patients older age groups. By continuous sampling of 90 patients taken away: the main group - 45 persons 60-86, comparison group - 45 persons 25-59 years. Found that for GERD in patients older than 60 years has its own characteristics. Maximum observed incidence of GERD in the range of 60-69 years (57%) with a further reduction. More typical is a decrease in the frequency of heartburn (p<0,05) with increasing retrosternal pain (p<0,001) and dysphagia (p<0,05) and coughing (p<0,001) with simultaneous increase in the number of complaints from various organs and systems. In this case, deterioration of health associated with a statistically significant reduction in quality of life parameters when compared with the young. When survey by questionnaire SF-36 in elderly patients reported a more marked reduction of scales that characterize the physical and psychological health of the component against high polymorbidity. Identified features of the flow of GERD in the elderly, may be useful for streamlining diagnosis and therapy.}, }
@article {pmid31177447, year = {2019}, author = {Zotova, TY and Lapaev, NN and Azova, MM and Blagonravov, ML and Gigani, OO and Ait Aissa, A and Denisova, AP}, title = {Distribution of Polymorphisms of the Renin-Angiotensin System Genes (ACE, AGT, and AGTR1), ITGB3, and FTO in Pregnant Patients with Hypertensive Disorders.}, journal = {Bulletin of experimental biology and medicine}, volume = {167}, number = {1}, pages = {74-78}, doi = {10.1007/s10517-019-04464-6}, pmid = {31177447}, issn = {1573-8221}, mesh = {Adult ; Alpha-Ketoglutarate-Dependent Dioxygenase FTO/*genetics ; Angiotensinogen/*genetics ; Female ; Humans ; Hypertension, Pregnancy-Induced/*genetics ; Integrin beta3/*genetics ; Peptidyl-Dipeptidase A/*genetics ; Polymorphism, Genetic/genetics ; Pregnancy ; Prospective Studies ; Receptor, Angiotensin, Type 1/*genetics ; Renin-Angiotensin System/*genetics/*physiology ; }, abstract = {The study included pregnant women aged 23-41 years with preeclampsia and gestation-associated arterial hypertension at weeks 27-40 and patients with essential arterial hypertension developing under conditions of the metabolic syndrome and without it. Frequency analysis of polymorphisms of the renin-angiotensin system genes (ACE, AGT, and AGTR1), ITGB3, FTO and their associations confirmed the syndrome nature of hypertensive disorders in pregnancy. The presence allele T of AGT gene and/or allele C of AGTR1 gene in the genotype of patients with preeclampsia was associated with higher BP and pressure load over 24 h. Allele D of ACE gene was also essential for BP parameters (pressure load) in patients with preeclampsia and gestation-associated arterial hypertension. Due to high genetic heterogeneity of the preeclampsia syndrome and genetic differences in the incidence of the studied gene polymorphisms in preeclampsia and gestation-associated arterial hypertension, no direct associations between these gestation disorders and polymorphic markers of the renin-angiotensin system genes can be established. However, polymorphisms of the renin-angiotensin system genes are essential for the 24-h dynamics of BP and pressure load under conditions of hypertensive disorders in pregnancy.}, }
@article {pmid31164119, year = {2019}, author = {Shebanits, K and Günther, T and Johansson, ACV and Maqbool, K and Feuk, L and Jakobsson, M and Larhammar, D}, title = {Copy number determination of the gene for the human pancreatic polypeptide receptor NPY4R using read depth analysis and droplet digital PCR.}, journal = {BMC biotechnology}, volume = {19}, number = {1}, pages = {31}, pmid = {31164119}, issn = {1472-6750}, mesh = {DNA Copy Number Variations/*genetics ; *Gene Dosage ; Genome, Human/genetics ; Genomics/methods ; Humans ; Polymerase Chain Reaction/*methods ; Receptors, Neuropeptide Y/*genetics ; Reproducibility of Results ; Sequence Analysis, DNA/*methods ; }, abstract = {BACKGROUND: Copy number variation (CNV) plays an important role in human genetic diversity and has been associated with multiple complex disorders. Here we investigate a CNV on chromosome 10q11.22 that spans NPY4R, the gene for the appetite-regulating pancreatic polypeptide receptor Y4. This genomic region has been challenging to map due to multiple repeated elements and its precise organization has not yet been resolved. Previous studies using microarrays were interpreted to show that the most common copy number was 2 per genome.<br><br>RESULTS: We have investigated 18 individuals from the 1000 Genomes project using the well-established method of read depth analysis and the new droplet digital PCR (ddPCR) method. We find that the most common copy number for NPY4R is 4. The estimated number of copies ranged from three to seven based on read depth analyses with Control-FREEC and CNVnator, and from four to seven based on ddPCR. We suggest that the difference between our results and those published previously can be explained by methodological differences such as reference gene choice, data normalization and method reliability. Three high-quality archaic human genomes (two Neanderthal and one Denisova) display four copies of the NPY4R gene indicating that a duplication occurred prior to the human-Neanderthal/Denisova split.<br><br>CONCLUSIONS: We conclude that ddPCR is a sensitive and reliable method for CNV determination, that it can be used for read depth calibration in CNV studies based on already available whole-genome sequencing data, and that further investigation of NPY4R copy number variation and its consequences are necessary due to the role of Y4 receptor in food intake regulation.}, }
@article {pmid31163991, year = {2019}, author = {Santander, C and Montinaro, F and Capelli, C}, title = {Searching for archaic contribution in Africa.}, journal = {Annals of human biology}, volume = {46}, number = {2}, pages = {129-139}, doi = {10.1080/03014460.2019.1624823}, pmid = {31163991}, issn = {1464-5033}, mesh = {Africa ; African Continental Ancestry Group/*genetics ; Animals ; DNA, Ancient/*analysis ; Genome, Human ; Hominidae/*genetics ; Humans ; *Hybridization, Genetic ; }, abstract = {Context: Africa's role in the narrative of human evolution is indisputably emphasised in the emergence of Homo sapiens. However, once humans dispersed beyond Africa, the history of those who stayed remains vastly under-studied, lacking the proper attention the birthplace of both modern and archaic humans deserves. The sequencing of Neanderthal and Denisovan genomes has elucidated evidence of admixture between archaic and modern humans outside of Africa, but has not aided efforts in answering whether archaic admixture happened within Africa. Objectives: This article reviews the state of research for archaic introgression in African populations and discusses recent insights into this topic. Methods: Gathering published sources and recently released preprints, this review reports on the different methods developed for detecting archaic introgression. Particularly it discusses how relevant these are when implemented on African populations and what findings these studies have shown so far. Results: Methods for detecting archaic introgression have been predominantly developed and implemented on non-African populations. Recent preprints present new methods considering African populations. While a number of studies using these methods suggest archaic introgression in Africa, without an African archaic genome to validate these results, such findings remain as putative archaic introgression. Conclusion: In light of the caveats with implementing current archaic introgression detection methods in Africa, we recommend future studies to concentrate on unravelling the complicated demographic history of Africa through means of ancient DNA where possible and through more focused efforts to sequence modern DNA from more representative populations across the African continent.}, }
@article {pmid31133435, year = {2019}, author = {Denisova, K}, title = {Failure to attune to language predicts autism in high risk infants.}, journal = {Brain and language}, volume = {194}, number = {}, pages = {109-120}, doi = {10.1016/j.bandl.2019.04.002}, pmid = {31133435}, issn = {1090-2155}, mesh = {Autism Spectrum Disorder/diagnostic imaging/*physiopathology ; Head Movements ; Humans ; Infant ; *Language Development ; Magnetic Resonance Imaging ; Sleep ; Speech Perception ; }, abstract = {Young humans are typically sensitive to evolutionarily important aspects of information in the surrounding environment in a way that makes us thrive. Seeking to probe the putative disruptions of this process in infancy, I examined the statistical character of head movements in 52 9-10 mo-old infants, half at high familial risk (HR) for Autism Spectrum Disorders (ASD), who underwent an fMRI scan while listening to words spoken with alternating stress patterns on syllables. Relative to low risk (LR) infants, HR infants, in particular those showing the least rapid receptive language progress, had significantly lower noise-to-signal levels and increased symmetry. A comparison of patterns during a native language and a sleep scan revealed the most atypical ordering of signatures on the 3 tasks in a subset of HR infants, suggesting that the biological mechanism of language development is least acquisitive in those HR infants who go on to develop ASD in toddlerhood.}, }
@article {pmid31102945, year = {2019}, author = {Denisova, K}, title = {Age attenuates noise and increases symmetry of head movements during sleep resting-state fMRI in healthy neonates, infants, and toddlers.}, journal = {Infant behavior &amp; development}, volume = {57}, number = {}, pages = {101317}, doi = {10.1016/j.infbeh.2019.03.008}, pmid = {31102945}, issn = {1934-8800}, mesh = {Age Factors ; Brain/*diagnostic imaging/*physiology ; Child, Preschool ; Cross-Sectional Studies ; Female ; Head Movements/*physiology ; Humans ; Infant ; Infant, Newborn ; Magnetic Resonance Imaging/*methods ; Male ; Noise ; Rest/*physiology ; Sleep/*physiology ; }, abstract = {Newborns produce spontaneous movements during sleep that are functionally important for their future development. This nuance has been previously studied using animal models and more recently using movement data from sleep resting-state fMRI (rs-fMRI) scans. Age-related trajectory of statistical features of spontaneous movements of the head is under-examined. This study quantitatively mapped a developmental trajectory of spontaneous head movements during an rs-fMRI scan acquired during natural sleep in 91 datasets from healthy children from ∼birth to 3 years old, using the Open Science Infancy Research upcycling protocol. The youngest participants studied, 2-3 week-old neonates, showed increased noise-to-signal levels as well as lower symmetry features of their movements; noise-to-signal levels were attenuated and symmetry was increased in the older infants and toddlers (all Spearman's rank-order correlations, P < 0.05). Thus, statistical features of spontaneous head movements become more symmetrical and less noisy from birth to ∼3 years in children. Because spontaneous movements during sleep in early life may trigger new neuronal activity in the cortex, the key outstanding question for in vivo, non-invasive neuroimaging studies in young children is not "How can we correct head movement better?" but rather: How can we represent all important sources of neuronal activity that shape functional connections in the still-developing human central nervous system?}, }
@article {pmid31090374, year = {2019}, author = {Kovalkova, NA and Ragino, YI and Scherbakova, LV and Hudyakovа, AD and Denisova, DV and Voevoda, MI}, title = {Relationships of arterial hypertension and reduced renal function in a population 25-45 years.}, journal = {Terapevticheskii arkhiv}, volume = {91}, number = {1}, pages = {64-70}, doi = {10.26442/00403660.2019.01.000032}, pmid = {31090374}, issn = {0040-3660}, mesh = {Adult ; Blood Pressure/physiology ; Blood Pressure Determination ; Creatinine/*blood ; Cross-Sectional Studies ; Female ; Glomerular Filtration Rate/*physiology ; Humans ; Hypertension/epidemiology/*physiopathology ; Kidney/*physiopathology ; Male ; Middle Aged ; Risk Factors ; Russia/epidemiology ; }, abstract = {AIM: To study relationships of reduced renal function with hypertension and other cardiometabolic risk factors in persons aged 25-45 years.<br><br>MATERIALS AND METHODS: A cross-sectional population study of one of the typical district of Novosibirsk (Russia) was performed during 2013-2016 years. The study included 468 men and 606 women aged 25-45 years. Blood pressure (BP), waist circumference (WC), blood lipids, glucose, creatinine were measured. Glomerular filtration rate (GFR) was calculated with the formula CKD-EPI. Hypertension was registered if blood pressure (BP) was ≥140/90 mm Hg, reduced kidney function - at GFR<90 ml/min/1.73 cm2.<br><br>RESULTS: Prevalence of hypertension among men was 28%, among women - 9%. The proportion of people with GFR<90 ml/min/1.73 cm2 among men was 9.8%, among women - 34%. Among all examined people GFRs <60 ml/min/1.73 cm2 was revealed in 0.3% only. The association of hypertension with reduced renal function was determined only in men. Based on results of multivariate linear regression analysis, a significant negative association of GFR with age was determined, there was no association of GFR with systolic BP (SBP) in either men or women. In men, inverse relationships of GFR with low-density lipoprotein cholesterol (LDL-С), triglycerides (TG), direct - with WC were determined. Significant inverse association of GFR with diastolic BP (DBP) was revealed only after exception of TG from the regression model. In women, GFR's inverse relationship with LDL-С and DBP was observed, and the direct - with WC. In stepwise analysis the validity of all associations was confirmed after exception of the association of GFR with WC in men.<br><br>CONCLUSION: In a population of 25-45 years a reduced GFR was associated with increased DBP; levels of LDL-С, TG showed negative association with GFR; in men increased TG levels were more important in reducing GFR than elevated DBP.}, }
@article {pmid31084661, year = {2019}, author = {Denisova, SA and Shchenkov, SV}, title = {New data on the nervous system of Cercaria parvicaudata Stunkard &amp; Shaw, 1931 (Trematoda: Renicolidae): revisiting old hypotheses.}, journal = {Journal of helminthology}, volume = {94}, number = {}, pages = {e52}, doi = {10.1017/S0022149X1900035X}, pmid = {31084661}, issn = {1475-2697}, mesh = {Animals ; Cercaria/*anatomy &amp; histology ; Microscopy, Electron, Scanning ; Nervous System/*anatomy &amp; histology/ultrastructure ; Sensory Receptor Cells/*ultrastructure ; Silver Nitrate ; Specimen Handling ; Staining and Labeling ; }, abstract = {Data on the interposition of the immunoreactive nerve cords in Cercaria parvicaudata Stunkard &amp; Shaw, 1931 (Trematoda: Renicolidae) and its chaetotaxy were obtained. The nervous system of C. parvicaudata was described using immunostaining of 5-hydroxytryptamine and FMRFamide immunoreactive nerve elements. The morphology and distribution of sensory receptors were analysed using scanning electron microscopy and the silver nitrate impregnation technique. Our integrated approach to the study of the nervous system revealed a clear colocalization of surface papillae with nerve cords and commissures in C. parvicaudata. The structure of the nervous system in C. parvicaudata differs partly from the classical model that defines the entire nomenclature of chaetotaxy.}, }
@article {pmid31043746, year = {2019}, author = {Chen, F and Welker, F and Shen, CC and Bailey, SE and Bergmann, I and Davis, S and Xia, H and Wang, H and Fischer, R and Freidline, SE and Yu, TL and Skinner, MM and Stelzer, S and Dong, G and Fu, Q and Dong, G and Wang, J and Zhang, D and Hublin, JJ}, title = {A late Middle Pleistocene Denisovan mandible from the Tibetan Plateau.}, journal = {Nature}, volume = {569}, number = {7756}, pages = {409-412}, doi = {10.1038/s41586-019-1139-x}, pmid = {31043746}, issn = {1476-4687}, mesh = {Altitude ; Animals ; Caves ; *Fossils ; Hominidae/*anatomy &amp; histology/classification ; Human Migration ; Humans ; Mandible/*anatomy &amp; histology ; Phylogeny ; Tibet ; Tooth/anatomy &amp; histology ; }, abstract = {Denisovans are members of a hominin group who are currently only known directly from fragmentary fossils, the genomes of which have been studied from a single site, Denisova Cave1-3 in Siberia. They are also known indirectly from their genetic legacy through gene flow into several low-altitude East Asian populations4,5 and high-altitude modern Tibetans6. The lack of morphologically informative Denisovan fossils hinders our ability to connect geographically and temporally dispersed fossil hominins from Asia and to understand in a coherent manner their relation to recent Asian populations. This includes understanding the genetic adaptation of humans to the high-altitude Tibetan Plateau7,8, which was inherited from the Denisovans. Here we report a Denisovan mandible, identified by ancient protein analysis9,10, found on the Tibetan Plateau in Baishiya Karst Cave, Xiahe, Gansu, China. We determine the mandible to be at least 160 thousand years old through U-series dating of an adhering carbonate matrix. The Xiahe specimen provides direct evidence of the Denisovans outside the Altai Mountains and its analysis unique insights into Denisovan mandibular and dental morphology. Our results indicate that archaic hominins occupied the Tibetan Plateau in the Middle Pleistocene epoch and successfully adapted to high-altitude hypoxic environments long before the regional arrival of modern Homo sapiens.}, }
@article {pmid31043736, year = {2019}, author = {Warren, M}, title = {Biggest Denisovan fossil yet spills ancient human's secrets.}, journal = {Nature}, volume = {569}, number = {7754}, pages = {16-17}, pmid = {31043736}, issn = {1476-4687}, }
@article {pmid30990461, year = {2019}, author = {Jones, M and Denisova, A and Mitchell, S and Owen, T}, title = {Acceptability of a Plasticity-Focused Serious Game Intervention for Posttraumatic Stress Disorder: User Requirements Analysis.}, journal = {JMIR serious games}, volume = {7}, number = {2}, pages = {e11909}, pmid = {30990461}, issn = {2291-9279}, abstract = {BACKGROUND: Trauma-focused cognitive behavioral therapy (TF-CBT) is a first-line treatment for posttraumatic stress disorder (PTSD). Despite a solid evidence base, TF-CBT response and attrition rates vary considerably. Plasticity-focused interventions, including the use of serious games, have the potential to improve TF-CBT response and treatment retention.<br><br>OBJECTIVE: The aim of this study was to assess the acceptability of a mobile phone-delivered plasticity-focused serious game to improve response to TF-CBT for PTSD, and carry out a user requirements analysis should the development of a prototype be warranted.<br><br>METHODS: We conducted 2 one-to-one interviews (n=2), one focus group involving service users who had received a diagnosis of PTSD (n=3) and one focus group involving psychological trauma service clinicians (n=4).<br><br>RESULTS: We found that the concept of a plasticity-focused mobile phone intervention for PTSD is acceptable to patients and clinicians. Service users and clinicians both believed that the usage should be guided by a therapist, and both contributed useful inputs regarding the audiovisual aspects of the proposed serious game. It was accepted that the game would not be suitable for all patients and that clinicians would need to appropriately prescribe the usage of the game.<br><br>CONCLUSIONS: The findings highlight the acceptability of the proposed serious game and clarify the user requirements for such an intervention. It is the intention of the authors to carry out a user experience evaluation using a prototype serious game in a clinical population.}, }
@article {pmid30990150, year = {2019}, author = {Ragino, YI and Shcherbakova, LV and Denisova, DV and Kuzminykh, NA and Yachmeneva, MP and Voevoda, MI}, title = {[Blood lipids and angina pectoris (by epidemiological cardiological Rose questionnaire) in the population of 25-45 years of Novosibirsk].}, journal = {Kardiologiia}, volume = {59}, number = {3S}, pages = {30-35}, doi = {10.18087/cardio.2600}, pmid = {30990150}, issn = {0022-9040}, mesh = {Adult ; *Angina Pectoris ; Cholesterol, HDL ; Cholesterol, LDL ; Cross-Sectional Studies ; Female ; Humans ; Lipids ; Male ; Middle Aged ; Risk Factors ; Surveys and Questionnaires ; Triglycerides ; }, abstract = {The aim of the study was to investigate the prevalence of angina pectoris (AP) according to the standardized epidemiological questionnaire of Rose in the population of 25-45 years of Novosibirsk and to identify its association with some lipid and non-lipid risk factors for coronary heart disease (CHD).<br><br>MATERIAL AND METHODS: Cross-sectional survey of the population aged 25-45 in Novosibirsk was carried out. The study included 1439 people (656 men and 783 women). Within the framework of the complex survey program, the standardized epidemiological questionnaire of Rose (WHO, 1984) was used. Blood levels of total cholesterol (total C), triglycerides (TG), low and high density lipoprotein cholesterol (LDL-C, HDL-C) were determinate by biochemical methods.<br><br>RESULTS: For all lipid indicators, significant differences were found between men and women. The levels of total C, TG and LDL-C were significantly higher, and the level of HDL-C was lower in men, than in women. According to the Rose questionnaire, out of 1439 people included in the study, 12 patients (0.8%) had AP (75% women). In persons with AP, blood levels of TG were 1.8 times higher, and the levels of HDL-C in blood was 1.2 times lower compared to persons without AP. Univariate analysis of associations of AP with CHD risk factors showed that the chance of developing angina pectoris in the population of 25-45 years was significantly increased in individuals with high blood TG levels (OR 3,515, DI 1,106-11,168, p = 0.039) and low HDL-C (OR 1,203, DI 1,054-1,372, p = 0.006). A natural, although statistically not significant (OR 3,165, p=0,055, due to the small number of groups with AP) increasing in the chance of developing AP in hypertension was detected.<br><br>CONCLUSION: In the young population of 25-45 years in Novosibirsk, elevated blood levels of TG, reduced levels of HDL-C, and hypertension were associated with AP, according to Rosecardiological questionnaire, which underlines the importance of conducting screening surveys of the young population to improve effective prevention and treatment of diseases.}, }
@article {pmid30990136, year = {2019}, author = {Kuznetsov, AA and Tsvetkova, EE and Denisova, DV and Ragino, YI and Voevoda, MI}, title = {[Central Aortic Pressure: Reference and Diagnostic Values].}, journal = {Kardiologiia}, volume = {59}, number = {3}, pages = {11-17}, doi = {10.18087/cardio.2019.3.10235}, pmid = {30990136}, issn = {0022-9040}, mesh = {Adult ; *Arterial Pressure ; Blood Pressure ; Blood Pressure Determination ; Female ; Humans ; *Hypertension ; Male ; Radial Artery ; Reference Values ; }, abstract = {OBJECTIVE: Practical application of central aortic pressure (CAP) parameters is limited by the absence of generally recognized reference and threshold diagnostic indices. The purpose of this work is to establish their values in the general population of Novosibirsk. Materials and Methods. A total of 327 people were examined: 155 men and 172 women aged 25-44 years from a representative sample from the general population of Novosibirsk. Applanation tonometry of the radial artery was performed by the SphygmoCor system. The reference values of CAP parameters were obtained by a nonparametric method according to the Clinical and Laboratory Standards Institute (CLSI) recommendations (95 % percentile interval with 2.5 % and 97.5 % cut-off points and their 90 % confidence intervals). Diagnostic thresholds and categories of CAP were determined as mean values depending on the categories of brachial arterial pressure (BP) and on the basis of risk estimates, as well as sensitivity and specificity values for left ventricular hypertrophy similar to risk and sensitivity and specificity values of threshold levels (categories) of brachial BP.<br><br>RESULTS: The reference values of the parameters of the CAP were: 18-43 mm Hg for pulse pressure; 5-24 mm Hg for the amplification of pulse pressure; - 8.8-40 % for the augmentation index. Diagnostic categories of CAP were determined to be: optimal - less than 110 / 80, normal - 110 / 80-114 / 84, high normal - 115 / 85-124 / 89, hypertension - more than 125 / 90 mm Hg.<br><br>CONCLUSION: The reference values, diagnostic thresholds and categories of parameters of CAP in the general population of Novosibirsk aged 25-44 years have been determined. It is expedient to further study them.}, }
@article {pmid30981557, year = {2019}, author = {Jacobs, GS and Hudjashov, G and Saag, L and Kusuma, P and Darusallam, CC and Lawson, DJ and Mondal, M and Pagani, L and Ricaut, FX and Stoneking, M and Metspalu, M and Sudoyo, H and Lansing, JS and Cox, MP}, title = {Multiple Deeply Divergent Denisovan Ancestries in Papuans.}, journal = {Cell}, volume = {177}, number = {4}, pages = {1010-1021.e32}, doi = {10.1016/j.cell.2019.02.035}, pmid = {30981557}, issn = {1097-4172}, mesh = {Animals ; Asian Continental Ancestry Group/genetics ; Biological Evolution ; Gene Flow ; Genetic Introgression/*genetics ; Genetic Variation/genetics ; Genome, Human/genetics ; Haplotypes/*genetics ; Hominidae/*genetics ; Humans ; Indonesia ; Neanderthals/genetics ; Oceania ; }, abstract = {Genome sequences are known for two archaic hominins-Neanderthals and Denisovans-which interbred with anatomically modern humans as they dispersed out of Africa. We identified high-confidence archaic haplotypes in 161 new genomes spanning 14 island groups in Island Southeast Asia and New Guinea and found large stretches of DNA that are inconsistent with a single introgressing Denisovan origin. Instead, modern Papuans carry hundreds of gene variants from two deeply divergent Denisovan lineages that separated over 350 thousand years ago. Spatial and temporal structure among these lineages suggest that introgression from one of these Denisovan groups predominantly took place east of the Wallace line and continued until near the end of the Pleistocene. A third Denisovan lineage occurs in modern East Asians. This regional mosaic suggests considerable complexity in archaic contact, with modern humans interbreeding with multiple Denisovan groups that were geographically isolated from each other over deep evolutionary time.}, }
@article {pmid30942856, year = {2019}, author = {Harris, DN and Ruczinski, I and Yanek, LR and Becker, LC and Becker, DM and Guio, H and Cui, T and Chilton, FH and Mathias, RA and O'Connor, TD}, title = {Evolution of Hominin Polyunsaturated Fatty Acid Metabolism: From Africa to the New World.}, journal = {Genome biology and evolution}, volume = {11}, number = {5}, pages = {1417-1430}, pmid = {30942856}, issn = {1759-6653}, support = {R01 AT008621/AT/NCCIH NIH HHS/United States ; R01 HL087698/HL/NHLBI NIH HHS/United States ; R01 HL112064/HL/NHLBI NIH HHS/United States ; U01 HL072518/HL/NHLBI NIH HHS/United States ; }, mesh = {Animals ; *Evolution, Molecular ; Fatty Acid Desaturases/*genetics/metabolism ; Fatty Acids, Unsaturated/*metabolism ; Hominidae/*genetics/metabolism ; Humans ; Indians, North American/genetics ; Selection, Genetic ; Siberia ; }, abstract = {The metabolic conversion of dietary omega-3 and omega-6 18 carbon (18C) to long chain (>20 carbon) polyunsaturated fatty acids (LC-PUFAs) is vital for human life. The rate-limiting steps of this process are catalyzed by fatty acid desaturase (FADS) 1 and 2. Therefore, understanding the evolutionary history of the FADS genes is essential to our understanding of hominin evolution. The FADS genes have two haplogroups, ancestral and derived, with the derived haplogroup being associated with more efficient LC-PUFA biosynthesis than the ancestral haplogroup. In addition, there is a complex global distribution of these haplogroups that is suggestive of Neanderthal introgression. We confirm that Native American ancestry is nearly fixed for the ancestral haplogroup, and replicate a positive selection signal in Native Americans. This positive selection potentially continued after the founding of the Americas, although simulations suggest that the timing is dependent on the allele frequency of the ancestral Beringian population. We also find that the Neanderthal FADS haplotype is more closely related to the derived haplogroup and the Denisovan clusters closer to the ancestral haplogroup. Furthermore, the derived haplogroup has a time to the most recent common ancestor of 688,474 years before present. These results support an ancient polymorphism, as opposed to Neanderthal introgression, forming in the FADS region during the Pleistocene with possibly differential selection pressures on both haplogroups. The near fixation of the ancestral haplogroup in Native American ancestry calls for future studies to explore the potential health risk of associated low LC-PUFA levels in these populations.}, }
@article {pmid30889271, year = {2019}, author = {Vyas, DN and Mulligan, CJ}, title = {Analyses of Neanderthal introgression suggest that Levantine and southern Arabian populations have a shared population history.}, journal = {American journal of physical anthropology}, volume = {169}, number = {2}, pages = {227-239}, doi = {10.1002/ajpa.23818}, pmid = {30889271}, issn = {1096-8644}, support = {BCS-1258965//National Science Foundation/International ; }, mesh = {Animals ; Arabia ; Gene Flow/genetics ; Gene Frequency/genetics ; *Genetics, Population ; History, Ancient ; Human Migration/*history ; Humans ; Middle East ; Neanderthals/*genetics ; Polymorphism, Single Nucleotide/genetics ; }, abstract = {OBJECTIVES: Modern humans are thought to have interbred with Neanderthals in the Near East soon after modern humans dispersed out of Africa. This introgression event likely took place in either the Levant or southern Arabia depending on the dispersal route out of Africa that was followed. In this study, we compare Neanderthal introgression in contemporary Levantine and southern Arabian populations to investigate Neanderthal introgression and to study Near Eastern population history.<br><br>MATERIALS AND METHODS: We analyzed genotyping data on >400,000 autosomal SNPs from seven Levantine and five southern Arabian populations and compared these data to those from populations from around the world including Neanderthal and Denisovan genomes. We used f4 and D statistics to estimate and compare levels of Neanderthal introgression between Levantine, southern Arabian, and comparative global populations. We also identified 1,581 putative Neanderthal-introgressed SNPs within our dataset and analyzed their allele frequencies as a means to compare introgression patterns in Levantine and southern Arabian genomes.<br><br>RESULTS: We find that Levantine and southern Arabian populations have similar levels of Neanderthal introgression to each other but lower levels than other non-Africans. Furthermore, we find that introgressed SNPs have very similar allele frequencies in the Levant and southern Arabia, which indicates that Neanderthal introgression is similarly distributed in Levantine and southern Arabian genomes.<br><br>DISCUSSION: We infer that the ancestors of contemporary Levantine and southern Arabian populations received Neanderthal introgression prior to separating from each other and that there has been extensive gene flow between these populations.}, }
@article {pmid30885783, year = {2019}, author = {Alexeeva, E and Dvoryakovskaya, T and Denisova, R and Sleptsova, T and Isaeva, K and Chomahidze, A and Fetisova, A and Mamutova, A and Alshevskaya, A and Gladkikh, V and Moskalev, A}, title = {Dynamics of concomitant therapy in children with juvenile idiopathic arthritis treated with etanercept and methotrexate.}, journal = {Pediatrics and neonatology}, volume = {60}, number = {5}, pages = {549-555}, doi = {10.1016/j.pedneo.2019.02.003}, pmid = {30885783}, issn = {2212-1692}, mesh = {Adolescent ; Anti-Inflammatory Agents, Non-Steroidal/therapeutic use ; Antirheumatic Agents/*therapeutic use ; Arthritis, Juvenile/*drug therapy ; Child ; Child, Preschool ; Drug Therapy, Combination ; Etanercept/administration &amp; dosage/*therapeutic use ; Female ; Humans ; Infant ; Male ; Methotrexate/administration &amp; dosage/*therapeutic use ; }, abstract = {BACKGROUND: Both the steroid- and NSAID-sparing effects of biologics in juvenile idiopathic arthritis (JIA) treatment are key aspects of the dynamics of patient's condition. The proper selection of biologics enables maximum treatment effectiveness and reduction of the dosage of concomitant therapy. Our aim was to study the dynamics of concomitant therapy during etanercept (ETA) and methotrexate (MTX) treatment in patients with JIA.<br><br>METHODS: This analysis included 215 JIA patients (63.3% females) showing sufficient response to main therapy. One hundred patients received MTX as main therapy, 24 received ETA monotherapy, and 91 received ETA þ MTX combination therapy. The dynamics of concomitant therapy were analyzed after 1 month, every 3 months during the first year, and every 6 months during the long-term follow-up (up to 5 years).<br><br>RESULTS: At the baseline, 24 (11.2%) patients received concomitant oral glucocorticoids (orGCs) and NSAIDs; the remaining 191 (88.8%) patients were treated with concomitant NSAIDs only. Within 1-year treatment, NSAIDs were discontinued in 162 (75.3%) patients. There were no significant differences in the dynamics of withdrawal of NSAIDs in patients who received and did not receive concomitant MTX. However, the percentage of treatment discontinuation in the MTX group was significantly lower compared to the other two groups (p < 0.001). Oral GCs were discontinued completely in 4 children (16.7%), and the dose of oral GCs was reduced in another 4 patients (16.7%). By the end of the follow-up period, 44 of 115 patients (38.3%) treated with ETA in combination with any concomitant therapy could switch to ETA monotherapy.<br><br>CONCLUSION: Therapy with ETA makes it possible to reduce the dosage or completely discontinue most concomitant medications (orGCs, NSAIDs, MTX) in a significant percentage of patients. This reduces the risk of development of NSAID- and GC-induced pathological conditions, while the effectiveness of therapy of the underlying condition remains high.}, }
@article {pmid30853019, year = {2019}, author = {Kovalkova, NA and Ragino, YI and Hudyakovа, AD and Denisova, DV and Voevoda, MI}, title = {.}, journal = {Kardiologiia}, volume = {59}, number = {2}, pages = {32-37}, doi = {10.18087/cardio.2019.2.10228}, pmid = {30853019}, issn = {0022-9040}, mesh = {Adult ; Blood Pressure ; Cross-Sectional Studies ; Female ; Humans ; *Hypertension ; Male ; Middle Aged ; Prevalence ; Russia ; Siberia ; }, abstract = {PURPOSE: to study blood pressure levels and the prevalence of hypertension in persons aged 25-45 years in Novosibirsk.<br><br>MATERIALS AND METHODS: A cross-sectional population study in one of typical areas of Novosibirsk was performed in 2013-2016. The study included 479 men and 612 women aged 25-45 years. Arterial hypertension was defined as blood pressure (BP) ≥140 / 90 mmHg according to Russian recommendations (2004). For analysis two age groups were distinguished: 25-34 years and 35-45 years.<br><br>RESULTS: Mean values of systolic and diastolic BP were significantly lower in women than in men in age groups. In men and women, the analyzed indicators were significantly higher in the older than in the younger age group. Mean values of pulse pressure in men were significantly higher than in women in both age groups, there were no differences in the analyzed index between age groups in either men or women. Optimal BP was more often recorded among women than among men in both age groups. The proportion of persons with normal BP among men was grate than among women in both age groups. In the age group 35-45 years compared with the younger group, in men there was a decrease in incidence of category with BP<140 / 90 mmHg, an increase of the proportion of persons with hypertension grades 1 and 2, the appearance of persons with grade 3 hypertension; in women - an increase of the proportion of individuals with normal, high-normal BP, and with grade 1 hypertension, appearance of individuals with grades 2 and 3 hypertension. Frequency of BP categories ≥140 / 90 mmHg in age group 25-34 years among men was 17.6 %, among women 3.1 % (p<0.0001); in age group 35-45 years among men - 34.7 %, among women - 12.5 % (p<0.0001).<br><br>CONCLUSIONS: Prevalence of hypertension in men was 28 %, in women - 9 %. Favorable tendencies of BP indicators in both sexes were revealed over a 30-year period, while gender differences did not change.}, }
@article {pmid30773169, year = {2019}, author = {Buttura, RV and Ramalho, J and Lima, THA and Donadi, EA and Veiga-Castelli, LC and Mendes-Junior, CT and Castelli, EC}, title = {HLA-F displays highly divergent and frequent haplotype lineages associated with different mRNA expression levels.}, journal = {Human immunology}, volume = {80}, number = {2}, pages = {112-119}, doi = {10.1016/j.humimm.2018.10.016}, pmid = {30773169}, issn = {1879-1166}, mesh = {Animals ; Brazil ; Gene Expression Regulation ; Gene Frequency ; Genetics, Population ; Haplotypes ; High-Throughput Nucleotide Sequencing ; Histocompatibility Antigens Class I/*genetics ; Humans ; Linkage Disequilibrium ; Neanderthals/genetics ; Polymorphism, Genetic ; Promoter Regions, Genetic/*genetics ; RNA, Messenger/*genetics ; }, abstract = {HLA-F is one of the most conserved loci among the HLA gene family. The exact function of HLA-F is still under investigation. HLA-F might present tolerogenic features, participate in the stabilization of HLA molecules in open conformation, and also participate in the recycling of HLA molecules. Here we evaluate the variability and haplotype structure of the HLA-F distal promoter segment (from -1893 to -943) and how this segment is correlated with the coding region. Variability at the promoter segment was surveyed in 196 Brazilian samples using second-generation sequencing. The HLA-F promoter region presents two major haplotype lineages. Most of the variable sites are in perfect linkage and associated with a single promoter haplotype, here named F∗distal-C. This haplotype is associated with F∗01:01:02 alleles, while alleles from the F∗01:01:01 or F∗01:03 groups present closely related promoter sequences. F∗distal-C is quite frequent in Brazil and in worldwide populations, with frequencies ranging from 8.41% at the Iberian Population in Spain to 34.34% in Vietnam. F∗distal-C is also present in Neanderthal and Denisovan samples. In silico analyses demonstrated that F∗distal-C presents a different transcription factor binding profile compared with other HLA-F promoters. Moreover, individuals carrying this haplotype present higher HLA-F mRNA expression levels. Functional studies are required to define the exact mechanism underlying this higher HLA-F mRNA expression level associated with F∗distal-C and F∗01:01:02 alleles.}, }
@article {pmid30772945, year = {2019}, author = {James, WPT and Johnson, RJ and Speakman, JR and Wallace, DC and Frühbeck, G and Iversen, PO and Stover, PJ}, title = {Nutrition and its role in human evolution.}, journal = {Journal of internal medicine}, volume = {285}, number = {5}, pages = {533-549}, doi = {10.1111/joim.12878}, pmid = {30772945}, issn = {1365-2796}, mesh = {Animals ; *Biological Evolution ; DNA, Mitochondrial/genetics ; Emigration and Immigration ; Hominidae/genetics/*physiology ; Humans ; Mutation ; *Nutritional Physiological Phenomena ; }, abstract = {Our understanding of human evolution has improved rapidly over recent decades, facilitated by large-scale cataloguing of genomic variability amongst both modern and archaic humans. It seems clear that the evolution of the ancestors of chimpanzees and hominins separated 7-9 million years ago with some migration out of Africa by the earlier hominins; Homo sapiens slowly emerged as climate change resulted in drier, less forested African conditions. The African populations expanded and evolved in many different conditions with slow mutation and selection rates in the human genome, but with much more rapid mutation occurring in mitochondrial DNA. We now have evidence stretching back 300 000 years of humans in their current form, but there are clearly four very different large African language groups that correlate with population DNA differences. Then, about 50 000-100 000 years ago a small subset of modern humans also migrated out of Africa resulting in a persistent signature of more limited genetic diversity amongst non-African populations. Hybridization with archaic hominins occurred around this time such that all non-African modern humans possess some Neanderthal ancestry and Melanesian populations additionally possess some Denisovan ancestry. Human populations both within and outside Africa also adapted to diverse aspects of their local environment including altitude, climate, UV exposure, diet and pathogens, in some cases leaving clear signatures of patterns of genetic variation. Notable examples include haemoglobin changes conferring resistance to malaria, other immune changes and the skin adaptations favouring the synthesis of vitamin D. As humans migrated across Eurasia, further major mitochondrial changes occurred with some interbreeding with ancient hominins and the development of alcohol intolerance. More recently, an ability to retain lactase persistence into adulthood has evolved rapidly under the environmental stimulus of pastoralism with the ability to husband lactating ruminants. Increased amylase copy numbers seem to relate to the availability of starchy foods, whereas the capacity to desaturase and elongate monounsaturated fatty acids in different societies seems to be influenced by whether there is a lack of supply of readily available dietary sources of long-chain polyunsaturated fatty acids. The process of human evolution includes genetic drift and adaptation to local environments, in part through changes in mitochondrial and nuclear DNA. These genetic changes may underlie susceptibilities to some modern human pathologies including folate-responsive neural tube defects, diabetes, other age-related pathologies and mental health disorders.}, }
@article {pmid30763487, year = {2018}, author = {Denisova, EI and Korolev, AA and Nikitenko, EI and Kirpichenkova, EV and Fetisov, RN and Kozlov, VV and Onishchenko, GG}, title = {[Hygienic assessment of indoles in the diet of medical students].}, journal = {Voprosy pitaniia}, volume = {87}, number = {6}, pages = {22-27}, doi = {10.24411/0042-8833-2018-10063}, pmid = {30763487}, issn = {0042-8833}, mesh = {Adult ; *Brassicaceae ; *Diet ; Female ; Humans ; Indoles/*administration &amp; dosage ; Male ; Moscow ; Neoplasms/ethnology/prevention &amp; control ; Retrospective Studies ; *Students, Medical ; *Surveys and Questionnaires ; *Vegetables ; }, abstract = {A number of studies have shown the relationship between the regular consumption of cruciferous vegetables and the risk of malignant tumors in certain localizations, the activation of mechanisms of alimentary adaptation of the organism under conditions of alien loads, by inducing enzymes of the biotransformation system of xenobiotics. The cruciferous vegetables are distinguished by the presence of minor components, such as indole-3-carbinol, formed during the hydrolysis of glucosinolates. The aim of the investigation was a retrospective study of the content of indoles in students' diet with subsequent quantitative analysis in different comparison groups. The study involved 250 students from a medical university aged 21 to 27 years. To assess the actual nutrition, the developed questionnaires were used, which included the most common products in the Moscow region, sources of indole glucosinolates. It was found that 44% of the respondents didn't include cruciferous vegetables in the diet, and of those who consumed sources of indoles (56% of respondents), only about half received them in the recommended amount. It should also be noted that as in men, in women the most commonly used in the diet product as a source of indoles was cabbage, it was included in the diet of 68% of the respondents who used cruciferous vegetables, rarely pekin cabbage was used (16.3%) and broccoli (16.3%). Cauliflower, radishes, Kale and horseradish was included in the diet of 7.8-14.9% of the students. Less often turnip was consumed - only by 2.1% of the students. No significant differences in the consumption of indoles in the student with deficient, normal or overweight was revealed. Also, there was no correlation between excess weight and the consumption of various indoles sources. The obtained results testify to the extremely low level of alimentary intake of indole-3-carbinol.}, }
@article {pmid30745996, year = {2019}, author = {Gringolts, ML and Denisova, YI and Finkelshtein, ES and Kudryavtsev, YV}, title = {Olefin metathesis in multiblock copolymer synthesis.}, journal = {Beilstein journal of organic chemistry}, volume = {15}, number = {}, pages = {218-235}, pmid = {30745996}, issn = {1860-5397}, abstract = {Multiblock copolymers constitute a basis for an emerging class of nanomaterials that combine various functional properties with durability and enhanced mechanical characteristics. Our mini-review addresses synthetic approaches to the design of multiblock copolymers from unsaturated monomers and polymers using olefin metathesis reactions and other ways of chemical modification across double C=C bonds. The main techniques, actively developed during the last decade and discussed here, are the coupling of end-functionalized blocks, sequential ring-opening metathesis polymerization, and cross metathesis between unsaturated polymers, or macromolecular cross metathesis. The last topic attracts special interest due to its relative simplicity and broad opportunities to tailor the structure and hence the properties of the copolymer products. Whenever possible, we analyze the structure-property relations for multiblock copolymers and point to their possible practical applications.}, }
@article {pmid30721182, year = {2018}, author = {Bulynko, SA and Denisova, OA and Soldatsky, YL and Severin, TV and Cherednichenko, AS}, title = {[The observation of two cases of isolated parapharyngeal abscess in a single child].}, journal = {Vestnik otorinolaringologii}, volume = {83}, number = {6}, pages = {44-45}, doi = {10.17116/otorino20188306144}, pmid = {30721182}, issn = {0042-4668}, mesh = {Child ; Child, Preschool ; Humans ; *Pharyngeal Diseases/diagnosis ; *Retropharyngeal Abscess/diagnosis ; Retrospective Studies ; }, abstract = {This article reports a rare observation of the simultaneous appearance of two isolated parapharyngeal abscesses in a 3 year-old child.}, }
@article {pmid30700881, year = {2019}, author = {Dennell, R}, title = {Dating of hominin discoveries at Denisova.}, journal = {Nature}, volume = {565}, number = {7741}, pages = {571-572}, doi = {10.1038/d41586-019-00264-0}, pmid = {30700881}, issn = {1476-4687}, mesh = {Animals ; Archaeology ; Caves ; *Fossils ; *Hominidae ; }, }
@article {pmid30700871, year = {2019}, author = {Douka, K and Slon, V and Jacobs, Z and Ramsey, CB and Shunkov, MV and Derevianko, AP and Mafessoni, F and Kozlikin, MB and Li, B and Grün, R and Comeskey, D and Devièse, T and Brown, S and Viola, B and Kinsley, L and Buckley, M and Meyer, M and Roberts, RG and Pääbo, S and Kelso, J and Higham, T}, title = {Age estimates for hominin fossils and the onset of the Upper Palaeolithic at Denisova Cave.}, journal = {Nature}, volume = {565}, number = {7741}, pages = {640-644}, doi = {10.1038/s41586-018-0870-z}, pmid = {30700871}, issn = {1476-4687}, mesh = {Animals ; Bayes Theorem ; *Caves ; DNA, Mitochondrial/genetics ; Deer ; Femur/chemistry ; *Fossils ; Geologic Sediments/chemistry ; History, Ancient ; *Hominidae/genetics ; Humans ; Neanderthals/genetics ; Oxygen Isotopes ; *Radiometric Dating ; Siberia ; Time Factors ; Tooth/chemistry ; }, abstract = {Denisova Cave in the Siberian Altai (Russia) is a key site for understanding the complex relationships between hominin groups that inhabited Eurasia in the Middle and Late Pleistocene epoch. DNA sequenced from human remains found at this site has revealed the presence of a hitherto unknown hominin group, the Denisovans1,2, and high-coverage genomes from both Neanderthal and Denisovan fossils provide evidence for admixture between these two populations3. Determining the age of these fossils is important if we are to understand the nature of hominin interaction, and aspects of their cultural and subsistence adaptations. Here we present 50 radiocarbon determinations from the late Middle and Upper Palaeolithic layers of the site. We also report three direct dates for hominin fragments and obtain a mitochondrial DNA sequence for one of them. We apply a Bayesian age modelling approach that combines chronometric (radiocarbon, uranium series and optical ages), stratigraphic and genetic data to calculate probabilistically the age of the human fossils at the site. Our modelled estimate for the age of the oldest Denisovan fossil suggests that this group was present at the site as early as 195,000 years ago (at 95.4% probability). All Neanderthal fossils-as well as Denisova 11, the daughter of a Neanderthal and a Denisovan4-date to between 80,000 and 140,000 years ago. The youngest Denisovan dates to 52,000-76,000 years ago. Direct radiocarbon dating of Upper Palaeolithic tooth pendants and bone points yielded the earliest evidence for the production of these artefacts in northern Eurasia, between 43,000 and 49,000 calibrated years before present (taken as AD 1950). On the basis of current archaeological evidence, it may be assumed that these artefacts are associated with the Denisovan population. It is not currently possible to determine whether anatomically modern humans were involved in their production, as modern-human fossil and genetic evidence of such antiquity has not yet been identified in the Altai region.}, }
@article {pmid30700870, year = {2019}, author = {Jacobs, Z and Li, B and Shunkov, MV and Kozlikin, MB and Bolikhovskaya, NS and Agadjanian, AK and Uliyanov, VA and Vasiliev, SK and O'Gorman, K and Derevianko, AP and Roberts, RG}, title = {Timing of archaic hominin occupation of Denisova Cave in southern Siberia.}, journal = {Nature}, volume = {565}, number = {7741}, pages = {594-599}, doi = {10.1038/s41586-018-0843-2}, pmid = {30700870}, issn = {1476-4687}, mesh = {Animals ; *Caves ; Geologic Sediments/chemistry ; History, Ancient ; *Hominidae ; Siberia ; Time Factors ; }, abstract = {The Altai region of Siberia was inhabited for parts of the Pleistocene by at least two groups of archaic hominins-Denisovans and Neanderthals. Denisova Cave, uniquely, contains stratified deposits that preserve skeletal and genetic evidence of both hominins, artefacts made from stone and other materials, and a range of animal and plant remains. The previous site chronology is based largely on radiocarbon ages for fragments of bone and charcoal that are up to 50,000 years old; older ages of equivocal reliability have been estimated from thermoluminescence and palaeomagnetic analyses of sediments, and genetic analyses of hominin DNA. Here we describe the stratigraphic sequences in Denisova Cave, establish a chronology for the Pleistocene deposits and associated remains from optical dating of the cave sediments, and reconstruct the environmental context of hominin occupation of the site from around 300,000 to 20,000 years ago.}, }
@article {pmid30672457, year = {2019}, author = {Henry, JP}, title = {[Genetics and origin of Homo sapiens].}, journal = {Medecine sciences : M/S}, volume = {35}, number = {1}, pages = {39-45}, doi = {10.1051/medsci/2018311}, pmid = {30672457}, issn = {1958-5381}, mesh = {Africa ; Animals ; Asia ; Biological Evolution ; DNA/analysis/chemistry ; Europe ; *Evolution, Molecular ; *Genome, Human ; Hominidae/*genetics ; Human Genome Project ; Humans ; Neanderthals/genetics ; Phylogeny ; Polymorphism, Single Nucleotide ; Selection, Genetic ; Sequence Homology ; }, abstract = {Usually, paleoanthropology studies remains and artefacts. However, more recently, genetics offer new avenues. Information on humanisation mechanisms has been obtained from comparison with primate or archaic Homo DNA sequences. Likewise, the 1 000 Genomes Project has characterized the geographic spectrum of human genetic variation offering a basis for a genomic study of Homo sapiens phylogeny. From these studies, a model, Out of Africa, was derived. His origin is Africa, where he lived 200 000 years ago. A small fraction of the population left Africa between 50 and 100 000 years ago that have populated the rest of the world, to Europe, coastal Asia to Australia and mainland Asia to Behring Land Bridge and America. The model is supported by the decrease of genetic diversity with the distance to Eastern Africa (serial founder effect). In Europe and Asia, Homo sapiens met archaic Homo neanderthalis and H denisova. The presence of 1-3% neanderthalis sequences in modern Homo ADN indicates admixtures between these groups. Some archaic sequences are on positive selection pressure, thus suggesting that the extinct hominins might have facilitated the adaptation of H sapiens to new environments.}, }
@article {pmid30651539, year = {2019}, author = {Mondal, M and Bertranpetit, J and Lao, O}, title = {Approximate Bayesian computation with deep learning supports a third archaic introgression in Asia and Oceania.}, journal = {Nature communications}, volume = {10}, number = {1}, pages = {246}, pmid = {30651539}, issn = {2041-1723}, abstract = {Since anatomically modern humans dispersed Out of Africa, the evolutionary history of Eurasian populations has been marked by introgressions from presently extinct hominins. Some of these introgressions have been identified using sequenced ancient genomes (Neanderthal and Denisova). Other introgressions have been proposed for still unidentified groups using the genetic diversity present in current human populations. We built a demographic model based on deep learning in an Approximate Bayesian Computation framework to infer the evolutionary history of Eurasian populations including past introgression events in Out of Africa populations fitting the current genetic evidence. In addition to the reported Neanderthal and Denisovan introgressions, our results support a third introgression in all Asian and Oceanian populations from an archaic population. This population is either related to the Neanderthal-Denisova clade or diverged early from the Denisova lineage. We propose the use of deep learning methods for clarifying situations with high complexity in evolutionary genomics.}, }
@article {pmid30592887, year = {2018}, author = {Kirpichenkova, EV and Korolev, AA and Onishchenko, GG and Nikitenko, EI and Lipatov, DV and Kuz'min, AG and Dyskin, YA and Denisova, EL and Fetisov, RN}, title = {[Study of lutein and zeaxanthin content in the diet with the assessment of the relationship between the level of alimentary intake of non-vitamin carotenoids and the density of the macular region of the retina at a young age].}, journal = {Voprosy pitaniia}, volume = {87}, number = {5}, pages = {20-26}, doi = {10.24411/0042-8833-2018-10049}, pmid = {30592887}, issn = {0042-8833}, mesh = {Adult ; *Carotenoids/administration &amp; dosage/analysis ; *Diet ; *Eating ; Female ; Food Analysis ; Humans ; *Lutein/administration &amp; dosage/analysis ; *Macula Lutea/diagnostic imaging/metabolism ; Male ; Retrospective Studies ; *Tomography, Optical Coherence ; *Zeaxanthins/administration &amp; dosage/analysis ; }, abstract = {Lutein and zeaxanthin are carotenoid pigments that affect the function of the visual analyzer. They selectively accumulate in the yellow spot of the retina, form macular pigment and determine the density of the retina macula. Lutein and zeaxanthin slow down the progression of age-related macular degeneration, a leading cause of senior-age blindness. The main food sources of non-vitamin carotenoids are green leafy vegetables, zucchini, pumpkin, green peas, broccoli. The aim of the study is a retrospective assessment of the levels and sources of alimentary intake of lutein and zeaxanthin in young people and research of the effect of lutein and zeaxanthin in the diet on macula density. A specially designed questionnaire was used to quantify the content of lutein and zeaxanthin in the diet, reflecting the amount of consumption of the main sources of these carotenoids on the day preceding the survey. A non-invasive non-contact method of optical coherence tomography of the retina was used to determine the density of the macula. The study involved 96 students of Sechenov University at the age of 21-27 years. The study found that only 6.25% of the respondents had daily intake of lutein and zeaxanthin of 6 mg or more, 8.33% had 4.6-5.9 mg, 8.33% had 3.0-4.5 mg, in 18.75% - 1.5-2.9 mg, in 45.83% <1.4 mg. 12.5% of respondents didn't include sources of lutein and zeaxanthin in the diet. The more common sources of lutein and zeaxanthin in the diet were eggs and fresh tomatoes. Retinal density indices corresponded to the age standards in the majority of the examined. In 8.3% surveyed the thickness of the retina was decreased, and 4.2% had higher thickness of the retina in comparison with the standards. Significant differences in the Central subfield thickness in men and women were revealed. There was no dependence of the levels of lutein and zeaxanthin coming from food sources on the retina thickness indicators.}, }
@article {pmid30592878, year = {2018}, author = {Martinchik, AN and Baeva, VS and Peskova, EV and Kudryavtseva, KV and Denisova, NN and Lavrinenko, SV and Kambarov, AO and Badtieva, VA and Nikityuk, DB}, title = {[Actual liquid consumption by highly qualified athletes in the mode of the training process].}, journal = {Voprosy pitaniia}, volume = {87}, number = {3}, pages = {36-44}, doi = {10.24411/0042-8833-2018-10029}, pmid = {30592878}, issn = {0042-8833}, mesh = {Adult ; *Athletes ; *Body Weight ; *Drinking Water ; *Energy Drinks ; *Energy Intake ; Humans ; Male ; *Nutrition Surveys ; }, abstract = {The purpose of the study was to evaluate the actual intake of fluids by athletes of various sports during the day with one and two training sessions before, during and after workout. The dietary intake, including consumption of various types of liquid foods and beverages, was evaluated by the method of 24-hour recall in 280 athletes of high qualification (candidates for masters and masters of sports) of both gender of various sports during the training period. It has been established that the main drink of rehydration was drinking bottled water. Bottled water was consumed on average by 86% of athletes. It was consumed by 95-96% of sportsmen from the group of single combats and power kinds, whereas in other groups the share of water consumers was less - 67-79%. In second place in terms of percentage of consuming was tea. Consumption of sports drinks was observed only during training by athletes from the group of cyclic sports (31%) and single combat (11%). Calculating the per capita fluid intake of athletes who had 2 workouts a day showed that athletes from the martial arts group consumed the largest volumes of fluid in the mode of both training sessions as compared to representatives of other sports. Athletes of other sports consumed on average less liquid in the 2nd training mode compared to the 1st one. The total fluid intake during two training sessions was maximum in the group of martial arts and was minimum in the group of complex coordination sports. It should be specially noted a small proportion of athletes who consumed specialized sports drinks - only 17% of athletes and more than half of them - cyclical sportsmen. Consumption of liquid food outside training has been observed in 76% of athletes. The mean volume of consumed liquid products varied by the user from 382 and 437 ml in complex coordination and game sports up to 504-553 ml in other sports. The daily fluid intake was maximum (2326 ml) in athletes engaged in martial arts, minimum (1009 ml) - in athletes of complex coordination sports.}, }
@article {pmid30566634, year = {2019}, author = {Reher, D and Key, FM and Andrés, AM and Kelso, J}, title = {Immune Gene Diversity in Archaic and Present-day Humans.}, journal = {Genome biology and evolution}, volume = {11}, number = {1}, pages = {232-241}, pmid = {30566634}, issn = {1759-6653}, mesh = {Animals ; Genetic Variation ; Humans ; Immunity, Innate/*genetics ; *Major Histocompatibility Complex ; Neanderthals/*genetics/immunology ; }, abstract = {Genome-wide analyses of two Neandertals and a Denisovan have shown that these archaic humans had lower genetic heterozygosity than present-day people. A similar reduction in genetic diversity of protein-coding genes (gene diversity) was found in exome sequences of three Neandertals. Reduced gene diversity, particularly in genes involved in immunity, may have important functional consequences. In fact, it has been suggested that reduced diversity in immune genes may have contributed to Neandertal extinction. We therefore explored gene diversity in different human groups, and at different time points on the Neandertal lineage, with a particular focus on the diversity of genes involved in innate immunity and genes of the Major Histocompatibility Complex (MHC).We find that the two Neandertals and a Denisovan have similar gene diversity, all significantly lower than any present-day human. This is true across gene categories, with no gene set showing an excess decrease in diversity compared with the genome-wide average. Innate immune-related genes show a similar reduction in diversity to other genes, both in present-day and archaic humans. There is also no observable decrease in gene diversity over time in Neandertals, suggesting that there may have been no ongoing reduction in gene diversity in later Neandertals, although this needs confirmation with a larger sample size. In both archaic and present-day humans, genes with the highest levels of diversity are enriched for MHC-related functions. In fact, in archaic humans the MHC genes show evidence of having retained more diversity than genes involved only in the innate immune system.}, }
@article {pmid30552618, year = {2018}, author = {Starshinova, A and Zinchenko, Y and Filatov, M and Denisova, N and Istomina, E and Landa, S and Burdakov, V and Churilov, L and Sapozhnikova, N and Pavlova, M and Stepanenko, T and Mayevskaya, V and Yablonskiy, P}, title = {Specific features of immune complexes in patients with sarcoidosis and pulmonary tuberculosis.}, journal = {Immunologic research}, volume = {66}, number = {6}, pages = {737-743}, pmid = {30552618}, issn = {1559-0755}, mesh = {Antigen-Antibody Complex/*immunology ; Diagnosis, Differential ; Humans ; Immunologic Tests/methods ; Lung/immunology ; Prospective Studies ; Sarcoidosis, Pulmonary/*diagnosis/*immunology ; Tuberculosis, Pulmonary/*diagnosis/*immunology ; }, abstract = {Clinical and radiological features of tuberculosis and sarcoidosis are quite overlapping, and therefore, a diagnostic dilemma often persists. There are no commonly accepted criteria for the diagnosis of sarcoidosis due to the lack of data on the etiology of the disease. The exclusion of tuberculosis in every patient with suspected sarcoidosis is a mandatory stage of diagnosis, especially in countries with a high burden of tuberculosis. A prospective study was conducted with two groups of patients: group I (n = 50)-patients with pulmonary sarcoidosis established according to standard criteria; group II (n = 28)-patients with pulmonary tuberculosis with bacterial excretion. The control group (n = 24) was presented by healthy subjects. The examination complex included x-ray, bacteriological, immunological (Mantoux test with 2 TE, TB.SPOT test), and histological methods. All patients and healthy subjects were assessed for immune complexes with the use of the dynamic light scattering (DLS) method and adding of "healthy lung tissue extract" antigens and specific tuberculosis antigens ESAT-6 and SFP-10 in vitro. Significant differences were found in determining specific immune complexes in patients with pulmonary sarcoidosis and pulmonary tuberculosis. Registration of specific immune complex formation with "healthy lung tissue extract" in 100% cases may indicate the autoimmune nature of sarcoidosis. The absence of the immune complex formation in response to ESAT-6/SFP-10 antigens can be used for the differential diagnosis of two diseases. The diagnostic significance of the DLS method was 100% for sarcoidosis and 92.2% for tuberculosis. The data obtained in the study allows not only understanding the etiology of sarcoidosis, but also obtaining new criteria for the differential diagnosis of tuberculosis and pulmonary sarcoidosis.}, }
@article {pmid30546947, year = {2019}, author = {Pol, JG and Acuna, SA and Yadollahi, B and Tang, N and Stephenson, KB and Atherton, MJ and Hanwell, D and El-Warrak, A and Goldstein, A and Moloo, B and Turner, PV and Lopez, R and LaFrance, S and Evelegh, C and Denisova, G and Parsons, R and Millar, J and Stoll, G and Martin, CG and Pomoransky, J and Breitbach, CJ and Bramson, JL and Bell, JC and Wan, Y and Stojdl, DF and Lichty, BD and McCart, JA}, title = {Preclinical evaluation of a MAGE-A3 vaccination utilizing the oncolytic Maraba virus currently in first-in-human trials.}, journal = {Oncoimmunology}, volume = {8}, number = {1}, pages = {e1512329}, pmid = {30546947}, issn = {2162-4011}, abstract = {Multiple immunotherapeutics have been approved for cancer patients, however advanced solid tumors are frequently refractory to treatment. We evaluated the safety and immunogenicity of a vaccination approach with multimodal oncolytic potential in non-human primates (NHP) (Macaca fascicularis). Primates received a replication-deficient adenoviral prime, boosted by the oncolytic Maraba MG1 rhabdovirus. Both vectors expressed the human MAGE-A3. No severe adverse events were observed. Boosting with MG1-MAGEA3 induced an expansion of hMAGE-A3-specific CD4+ and CD8+ T-cells with the latter peaking at remarkable levels and persisting for several months. T-cells reacting against epitopes fully conserved between simian and human MAGE-A3 were identified. Humoral immunity was demonstrated by the detection of circulating MAGE-A3 antibodies. These preclinical data establish the capacity for the Ad:MG1 vaccination to engage multiple effector immune cell populations without causing significant toxicity in outbred NHPs. Clinical investigations utilizing this program for the treatment of MAGE-A3-positive solid malignancies are underway (NCT02285816, NCT02879760).}, }
@article {pmid30538215, year = {2018}, author = {Denisova, TG and Gerasimova, LI and Pakhmutova, NL and Mahesh, S and Vithoulkas, G}, title = {Individualized Homeopathic Therapy in a Case of Obesity, Dysfunctional Uterine Bleeding, and Autonomic Dystonia.}, journal = {The American journal of case reports}, volume = {19}, number = {}, pages = {1474-1479}, pmid = {30538215}, issn = {1941-5923}, mesh = {Adult ; Dystonia/complications/*therapy ; Female ; *Homeopathy ; Humans ; Metrorrhagia/complications/*therapy ; Obesity/complications/*therapy ; }, abstract = {BACKGROUND Obesity is one of the leading causes of morbidity and mortality globally and challenging to treat because of the multifactorial etiology and presentation. Individualized homeopathy takes into account factors that led to a patient's health condition and hence may have a role in the treatment of obesity and related co-morbidities; co-morbidities that may arising from the same etiology may respond as a whole to homeopathy treatment. CASE REPORT A 39-year-old Russian female who developed multiple problems after severe emotional stress was treated with individualized classical homeopathic therapy. Obesity, dysfunctional uterine bleeding, and dysautonomia were pathologies that showed improvement. CONCLUSIONS The response in this patient's case, supports the need for further investigation on the relevance of individualized homeopathy in these related conditions.}, }
@article {pmid30450552, year = {2019}, author = {Safronova, EI and Dydykin, SS and Grigorevskiy, ED and Tverye, EA and Kolchenko, SI and Piskunova, NN and Denisova, AV and Titova, GP and Parshin, VD and Romanova, OA and Panteleyev, AA}, title = {Experimental animal model for assessment of tracheal epithelium regeneration.}, journal = {The Laryngoscope}, volume = {129}, number = {6}, pages = {E213-E219}, doi = {10.1002/lary.27480}, pmid = {30450552}, issn = {1531-4995}, mesh = {Animals ; Disease Models, Animal ; Epithelium/*pathology ; Rabbits ; Regeneration/*physiology ; Respiratory Mucosa/*pathology ; Tissue Engineering/*methods ; *Tissue Scaffolds ; Trachea/injuries/pathology/*surgery ; Tracheal Diseases/pathology/*surgery ; }, abstract = {OBJECTIVES/HYPOTHESIS: To develop an experimental model in rabbits for assessment of tracheal epithelium regeneration through application of either natural or artificial polymer scaffolds.<br><br>STUDY DESIGN: First, we identified the size of full-thickness mucosal defect, which does not allow self-healing (a "critical defect"), thus representing an adequate experimental model for regenerative therapy of tracheal epithelium damage. Then, two methods of polymer scaffold fixation at the site of the epithelium defect were compared: suturing and fixation with a stent. This was done through: 1) formation of a full-thickness anterolateral mucosal defect by tracheal mucosa excision; and 2) fixation of the scaffold at the site of the tracheal epithelium defect using sutures (through a tracheal wall "window") or a vascular stent (through a small tracheal incision).<br><br>RESULTS: The dimension of a critical anterolateral mucosal defect of the trachea for rabbits was found to be 1.5 cm in length and more than 50% of the tracheal circumference. Fixation of the scaffold with a stent proved to be more efficient due to a uniform distribution of the pressure over the entire surface of the scaffold, whereas the suturing of the scaffold provided unsatisfactory results. In addition, fixation of the scaffold by suturing required formation of a large "window" in the tracheal wall. Thus, using the stent appeared to be technically less complicated and much less traumatic as compared to suturing.<br><br>CONCLUSION: We present an experimental in vivo animal model of tracheal epithelium injury and recovery. It can be effectively used with certain further modifications as a basis for routine testing of bioengineered constructs.<br><br>LEVEL OF EVIDENCE: NA Laryngoscope, 129:E213-E219, 2019.}, }
@article {pmid30362431, year = {2018}, author = {Malinova, LI and Furman, NV and Dolotovskaya, PV and Chernousova, LA and Denisova, TP}, title = {[ADP-Induced Recalcified Blood Clotting Time as a Marker of Rethrombosis Risk and Effectiveness of Antiplatelet Therapy in Acute Coronary Syndrome].}, journal = {Kardiologiia}, volume = {58}, number = {6}, pages = {5-12}, pmid = {30362431}, issn = {0022-9040}, mesh = {Acute Coronary Syndrome/*blood/complications/*drug therapy ; Adenosine Diphosphate/blood ; Adult ; Aspirin/therapeutic use ; Biomarkers ; Humans ; Male ; Platelet Aggregation Inhibitors/adverse effects/*therapeutic use ; Recurrence ; Risk Factors ; Thrombosis/etiology/*prevention &amp; control ; *Whole Blood Coagulation Time ; }, abstract = {PURPOSE: to assess the possibility of the use of ADP induced blood-clotting time measurement in clinical practice prognostication of the course of acute coronary syndrome (ACS) and assessment of effectiveness of antiplatelet therapy (APT).<br><br>MATERIALS AND METHODS: We enrolled in the study 163 male patients admitted to the coronary unit for acute coronary syndrome (ACS) and 38 male practically healthy volunteers (PHV). ADP induced blood-clotting time (ADP BCT) was measured as time (sec) between addition of ADP (10 μcmol) to recalcificated sample of citrate blood and clot formation. In healthy volunteers ADP BCT was determined before and 45 minutes after oral administration of acetylsalicylic acid (ASA, 250 mg). Risk of cardiovascular death was calculated using the GRACE score. Platelet function tests were performed by optical aggregometry. Follow-up period for patients with ACS was 24 months. The primary end point (PEP) was the composite of cardiovascular death and rehospitalization.<br><br>RESULTS: In ACS patients ADP BCT was significantly lower than in PHV: 134.8 (109.9; 161.3) vs 85.7 (60.5; 108.7) sec, p=0.015. In PHV ASA increased ADP BCT - 103.2 (95.1; 130.7) vs 133.1 (102.8; 154.3) sec, p=0.041. ADP BCT correlated with age in both PHV and patients (R= -0.431, p.}, }
@article {pmid32598648, year = {2018}, author = {Mustafina, SV and Ovsyannikova, AK and Voevoda, MI and Denisova, DV and Sherbakova, LV and Rymar, OD}, title = {[The prevalence of components of metabolic syndrome in the patients with diabetes melitus type 2 and mody diabetes in young people of Novosibirsk].}, journal = {Terapevticheskii arkhiv}, volume = {90}, number = {10}, pages = {55-59}, doi = {10.26442/terarkh201890104-59}, pmid = {32598648}, issn = {0040-3660}, abstract = {AIM: To estimate the prevalence of type 2 diabetes mellitus (DM2) and MODY diabetes as well as the prevalence of metabolic syndrome (MS) components for these types of diabetes in the young population of the city of Novosibirsk.<br><br>MATERIALS AND METHODS: In 2013-2017 years a population survey was conducted of a random representative sample of the population of 25-45 years of both sexes, residents of one of the typical districts of Novosibirsk. WHO criteria (1999-2013) were used for the diagnosis of diabetes: fasting blood glucose ≥7.0 mmol / l after an 8-hour fasting. Also group with DM2 included persons with a fasting blood glucose level.}, }
@article {pmid30289977, year = {2019}, author = {Johannessen, TC and Hasan-Olive, MM and Zhu, H and Denisova, O and Grudic, A and Latif, MA and Saed, H and Varughese, JK and Røsland, GV and Yang, N and Sundstrøm, T and Nordal, A and Tronstad, KJ and Wang, J and Lund-Johansen, M and Simonsen, A and Janji, B and Westermarck, J and Bjerkvig, R and Prestegarden, L}, title = {Thioridazine inhibits autophagy and sensitizes glioblastoma cells to temozolomide.}, journal = {International journal of cancer}, volume = {144}, number = {7}, pages = {1735-1745}, doi = {10.1002/ijc.31912}, pmid = {30289977}, issn = {1097-0215}, mesh = {Animals ; Antineoplastic Combined Chemotherapy Protocols/*administration &amp; dosage/pharmacology ; Autophagosomes/drug effects ; Autophagy/*drug effects ; Brain Neoplasms/*drug therapy/genetics ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Drug Resistance, Neoplasm/drug effects ; Drug Synergism ; Glioblastoma/*drug therapy/genetics ; Humans ; Lysosomes/drug effects ; Mice ; Synthetic Lethal Mutations ; Temozolomide/*administration &amp; dosage/therapeutic use ; Thioridazine/*administration &amp; dosage/pharmacology ; Xenograft Model Antitumor Assays ; }, abstract = {Glioblastoma multiforme (GBM) has a poor prognosis with an overall survival of 14-15 months after surgery, radiation and chemotherapy using temozolomide (TMZ). A major problem is that the tumors acquire resistance to therapy. In an effort to improve the therapeutic efficacy of TMZ, we performed a genome-wide RNA interference (RNAi) synthetic lethality screen to establish a functional gene signature for TMZ sensitivity in human GBM cells. We then queried the Connectivity Map database to search for drugs that would induce corresponding changes in gene expression. By this approach we identified several potential pharmacological sensitizers to TMZ, where the most potent drug was the established antipsychotic agent Thioridazine, which significantly improved TMZ sensitivity while not demonstrating any significant toxicity alone. Mechanistically, we show that the specific chemosensitizing effect of Thioridazine is mediated by impairing autophagy, thereby preventing adaptive metabolic alterations associated with TMZ resistance. Moreover, we demonstrate that Thioridazine inhibits late-stage autophagy by impairing fusion between autophagosomes and lysosomes. Finally, Thioridazine in combination with TMZ significantly inhibits brain tumor growth in vivo, demonstrating the potential clinical benefits of compounds targeting the autophagy-lysosome pathway. Our study emphasizes the feasibility of exploiting drug repurposing for the design of novel therapeutic strategies for GBM.}, }
@article {pmid30278065, year = {2018}, author = {Hoover, KC}, title = {Intragenus (Homo) variation in a chemokine receptor gene (CCR5).}, journal = {PloS one}, volume = {13}, number = {10}, pages = {e0204989}, pmid = {30278065}, issn = {1932-6203}, mesh = {Animals ; Gene Expression Regulation ; *Genetic Variation ; Hominidae/*genetics ; Humans ; Receptors, CCR5/*genetics ; Species Specificity ; }, abstract = {Humans have a comparatively higher rate of more polymorphisms in regulatory regions of the primate CCR5 gene, an immune system gene with both general and specific functions. This has been interpreted as allowing flexibility and diversity of gene expression in response to varying disease loads. A broad expression repertoire is useful to humans-the only globally distributed primate-due to our unique adaptive pattern that increased pathogen exposure and disease loads (e.g., sedentism, subsistence practices). The main objective of the study was to determine if the previously observed human pattern of increased variation extended to other members of our genus, Homo. The data for this study are mined from the published genomes of extinct hominins (four Neandertals and two Denisovans), an ancient human (Ust'-Ishim), and modern humans (1000 Genomes). An average of 15 polymorphisms per individual were found in human populations (with a total of 262 polymorphisms). There were 94 polymorphisms identified across extinct Homo (an average of 13 per individual) with 41 previously observed in modern humans and 53 novel polymorphisms (32 in Denisova and 21 in Neandertal). Neither the frequency nor distribution of polymorphisms across gene regions exhibit significant differences within the genus Homo. Thus, humans are not unique with regards to the increased frequency of regulatory polymorphisms and the evolution of variation patterns across CCR5 gene appears to have originated within the genus. A broader evolutionary perspective on regulatory flexibility may be that it provided an advantage during the transition to confrontational foraging (and later hunting) that altered human-environment interaction as well as during migration to Eurasia and encounters with novel pathogens.}, }
@article {pmid30226838, year = {2018}, author = {Skov, L and Hui, R and Shchur, V and Hobolth, A and Scally, A and Schierup, MH and Durbin, R}, title = {Detecting archaic introgression using an unadmixed outgroup.}, journal = {PLoS genetics}, volume = {14}, number = {9}, pages = {e1007641}, pmid = {30226838}, issn = {1553-7404}, support = {207492/Z/17/Z/WT_/Wellcome Trust/United Kingdom ; WT207492/WT_/Wellcome Trust/United Kingdom ; WT206194/WT_/Wellcome Trust/United Kingdom ; }, mesh = {African Continental Ancestry Group/genetics ; Animals ; Asian Continental Ancestry Group/genetics ; European Continental Ancestry Group/genetics ; Fossils ; Genome, Human/*genetics ; Hominidae/*genetics ; Humans ; Hybridization, Genetic/*genetics ; Neanderthals/*genetics ; Oceanic Ancestry Group/genetics ; Phylogeny ; }, abstract = {Human populations outside of Africa have experienced at least two bouts of introgression from archaic humans, from Neanderthals and Denisovans. In Papuans there is prior evidence of both these introgressions. Here we present a new approach to detect segments of individual genomes of archaic origin without using an archaic reference genome. The approach is based on a hidden Markov model that identifies genomic regions with a high density of single nucleotide variants (SNVs) not seen in unadmixed populations. We show using simulations that this provides a powerful approach to identifying segments of archaic introgression with a low rate of false detection, given data from a suitable outgroup population is available, without the archaic introgression but containing a majority of the variation that arose since initial separation from the archaic lineage. Furthermore our approach is able to infer admixture proportions and the times both of admixture and of initial divergence between the human and archaic populations. We apply the model to detect archaic introgression in 89 Papuans and show how the identified segments can be assigned to likely Neanderthal or Denisovan origin. We report more Denisovan admixture than previous studies and find a shift in size distribution of fragments of Neanderthal and Denisovan origin that is compatible with a difference in admixture time. Furthermore, we identify small amounts of Denisova ancestry in South East Asians and South Asians.}, }
@article {pmid30209350, year = {2018}, author = {Clyde, D}, title = {The girl with Neanderthal and Denisovan parents.}, journal = {Nature reviews. Genetics}, volume = {19}, number = {11}, pages = {668-669}, doi = {10.1038/s41576-018-0054-6}, pmid = {30209350}, issn = {1471-0064}, mesh = {Fathers ; Female ; Genome ; Humans ; Male ; Mothers ; Neanderthals/*genetics ; Parents ; }, }
@article {pmid30185153, year = {2018}, author = {Dergunova, LV and Filippenkov, IB and Stavchansky, VV and Denisova, AE and Yuzhakov, VV and Mozerov, SA and Gubsky, LV and Limborska, SA}, title = {Genome-wide transcriptome analysis using RNA-Seq reveals a large number of differentially expressed genes in a transient MCAO rat model.}, journal = {BMC genomics}, volume = {19}, number = {1}, pages = {655}, pmid = {30185153}, issn = {1471-2164}, support = {16-14-00077//Russian Science Foundation/ ; }, mesh = {Animals ; Brain/diagnostic imaging/metabolism ; Disease Models, Animal ; *Gene Expression Profiling ; Infarction, Middle Cerebral Artery/complications/diagnostic imaging/*genetics/pathology ; Magnetic Resonance Imaging ; Rats ; Reperfusion Injury/complications ; *Sequence Analysis, RNA ; Signal Transduction/genetics ; }, abstract = {BACKGROUND: The transient middle cerebral artery occlusion (tMCAO) model is used for studying the molecular mechanisms of ischemic damage and neuroprotection. Numerous studies have demonstrated the role of individual genes and associated signaling pathways in the pathogenesis of ischemic stroke. Here, the tMCAO model was used to investigate the genome-wide response of the transcriptome of rat brain tissues to the damaging effect of ischemia and subsequent reperfusion.<br><br>RESULTS: Magnetic resonance imaging and histological examination showed that the model of focal ischemia based on endovascular occlusion of the right middle cerebral artery for 90 min using a monofilament, followed by restoration of the blood flow, led to reproducible localization of ischemic damage in the subcortical structures of the brain. High-throughput RNA sequencing (RNA-Seq) revealed the presence of differentially expressed genes (DEGs) in subcortical structures of rat brains resulting from hemisphere damage by ischemia after tMCAO, as well as in the corresponding parts of the brains of sham-operated animals. Real-time reverse transcription polymerase chain reaction expression analysis of 20 genes confirmed the RNA-Seq results. We identified 469 and 1939 genes that exhibited changes in expression of > 1.5-fold at 4.5 and 24 h after tMCAO, respectively. Interestingly, we found 2741 and 752 DEGs under ischemia-reperfusion and sham-operation conditions at 24 h vs. 4.5 h after tMCAO, respectively. The activation of a large number of genes involved in inflammatory, immune and stress responses, apoptosis, ribosome function, DNA replication and other processes was observed in ischemia-reperfusion conditions. Simultaneously, massive down-regulation of the mRNA levels of genes involved in the functioning of neurotransmitter systems was recorded. A Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that dozens of signaling pathways were associated with DEGs in ischemia-reperfusion conditions.<br><br>CONCLUSIONS: The data obtained revealed a global profile of gene expression in the rat brain sub-cortex under tMCAO conditions that can be used to identify potential therapeutic targets in the development of new strategies for the prevention and treatment of ischemic stroke.}, }
@article {pmid30149747, year = {2019}, author = {Alexeeva, E and Dvoryakovskaya, T and Denisova, R and Sleptsova, T and Isaeva, K and Chomahidze, A and Fetisova, A and Mamutova, A and Alshevskaya, A and Gladkikh, V and Moskalev, A}, title = {Comparative analysis of the etanercept efficacy in children with juvenile idiopathic arthritis under the age of 4 years and children of older age groups using the propensity score matching method.}, journal = {Modern rheumatology}, volume = {29}, number = {5}, pages = {848-855}, doi = {10.1080/14397595.2018.1516329}, pmid = {30149747}, issn = {1439-7609}, mesh = {Adolescent ; Age Factors ; Aged ; Antirheumatic Agents/*therapeutic use ; Arthritis, Juvenile/*drug therapy ; Child ; Child, Preschool ; Etanercept/*therapeutic use ; Female ; Humans ; Male ; Propensity Score ; }, abstract = {Objective: The aim of this study was to analyze the efficacy and safety of etanercept (ETA) in children with juvenile idiopathic arthritis (JIA) under the age of 4 years and to compare the data with those for older age groups. Methods: Three groups comprising 34 patients each (total of 102 patients) were selected using the propensity score matching (PSM) method. The study group (patients under the age of 4 years; the Junior group (JNR)) was compared with patients of the older age groups, adjusted for criteria such as gender, JIA category, JIA severity, and either age at disease onset (the Reference by Age of disease Onset (RAO) group) or disease duration (the Reference by Disease Duration (RDD) group). Results: All three groups showed a good response to ETA therapy. During the follow-up period, only 4 (3.9%) patients failed to reach American College of Rheumatology (ACR) Pediatric criteria improvement at ACR50 level. In the JNR group, 82.4% of patients achieved ACR90 within a median time of 3 months (IQR, 3-6 months), which was a better result compared to the other two groups: 61.8% (RAO group) and 58.8% (RDD group) of patients achieved ACR90 within 6 (Interquartile Range (IQR), 3-9) months (p = .028). Three (9%) patients in the JNR group and none of the RDD and RAO groups discontinued treatment because of clinical remission (p = .045). Conclusion: An analysis of the ETA efficacy in different age groups comparable in terms of the diagnosis and disease severity demonstrated a higher efficacy of earlier ETA therapy in children of the same age at disease onset. In children at the early stage of arthritis (≤ 2.5 years long), ETA was more efficient in those with an earlier disease onset.}, }
@article {pmid30135579, year = {2018}, author = {Slon, V and Mafessoni, F and Vernot, B and de Filippo, C and Grote, S and Viola, B and Hajdinjak, M and Peyrégne, S and Nagel, S and Brown, S and Douka, K and Higham, T and Kozlikin, MB and Shunkov, MV and Derevianko, AP and Kelso, J and Meyer, M and Prüfer, K and Pääbo, S}, title = {The genome of the offspring of a Neanderthal mother and a Denisovan father.}, journal = {Nature}, volume = {561}, number = {7721}, pages = {113-116}, pmid = {30135579}, issn = {1476-4687}, support = {324139//European Research Council/International ; 694707//European Research Council/International ; 715069//European Research Council/International ; }, mesh = {Alleles ; Animals ; Fathers ; Female ; Gene Flow/genetics ; Genome ; Genomics ; History, Ancient ; Hominidae/*genetics ; Humans ; Hybridization, Genetic/*genetics ; Male ; Mothers ; Neanderthals/*genetics ; Time Factors ; }, abstract = {Neanderthals and Denisovans are extinct groups of hominins that separated from each other more than 390,000 years ago1,2. Here we present the genome of 'Denisova 11', a bone fragment from Denisova Cave (Russia)3 and show that it comes from an individual who had a Neanderthal mother and a Denisovan father. The father, whose genome bears traces of Neanderthal ancestry, came from a population related to a later Denisovan found in the cave4-6. The mother came from a population more closely related to Neanderthals who lived later in Europe2,7 than to an earlier Neanderthal found in Denisova Cave8, suggesting that migrations of Neanderthals between eastern and western Eurasia occurred sometime after 120,000 years ago. The finding of a first-generation Neanderthal-Denisovan offspring among the small number of archaic specimens sequenced to date suggests that mixing between Late Pleistocene hominin groups was common when they met.}, }
@article {pmid30135540, year = {2018}, author = {Warren, M}, title = {Mum's a Neanderthal, Dad's a Denisovan: First discovery of an ancient-human hybrid.}, journal = {Nature}, volume = {560}, number = {7719}, pages = {417-418}, doi = {10.1038/d41586-018-06004-0}, pmid = {30135540}, issn = {1476-4687}, }
@article {pmid30121933, year = {2018}, author = {Zotova, TY and Blagonravov, ML and Lapaev, NN and Denisova, AP}, title = {Hemodynamic Allostasis of Pregnant Women against the Background of Preeclampsia.}, journal = {Bulletin of experimental biology and medicine}, volume = {165}, number = {4}, pages = {440-444}, doi = {10.1007/s10517-018-4189-4}, pmid = {30121933}, issn = {1573-8221}, mesh = {Adult ; Allostasis ; Blood Pressure/physiology ; Circadian Rhythm ; Female ; Heart Rate/physiology ; Hemodynamics/*physiology ; Humans ; Hypertension/physiopathology ; Pre-Eclampsia/*physiopathology ; Pregnancy ; Young Adult ; }, abstract = {We analyzed diurnal hemodynamic parameters (HR, systolic BP, and diastolic BP) recorded from two groups of edematous and preeclamptic pregnant women. The unidirectional character of changes in the control over the functional state of cardiovascular system was revealed except for the indices, which mark a pathological process: elevated diurnal BP in preeclampsia and diminished percentage of oscillation power in edematous patients. Uniformity of the regulatory changes in patients with and without arterial hypertension can be viewed as manifestation of allostasis developed by the cardiovascular system during pregnancy. In preeclampsia, the greater allostatic load was reflected by the changes in diurnal, daytime, and nighttime BP and in the circadian index calculated for HR, systolic BP, and diastolic BP. In edematous patients, elevation of allostatic load was indicated by the percentage of ultradian rhythms.}, }
@article {pmid30076299, year = {2018}, author = {Helsen, CW and Hammill, JA and Lau, VWC and Mwawasi, KA and Afsahi, A and Bezverbnaya, K and Newhook, L and Hayes, DL and Aarts, C and Bojovic, B and Denisova, GF and Kwiecien, JM and Brain, I and Derocher, H and Milne, K and Nelson, BH and Bramson, JL}, title = {The chimeric TAC receptor co-opts the T cell receptor yielding robust anti-tumor activity without toxicity.}, journal = {Nature communications}, volume = {9}, number = {1}, pages = {3049}, pmid = {30076299}, issn = {2041-1723}, mesh = {Adoptive Transfer ; Animals ; CD28 Antigens/immunology ; Cell Line, Tumor ; Cytokines/blood/metabolism ; Cytotoxicity, Immunologic ; Female ; Genetic Engineering ; HEK293 Cells ; Humans ; Immunotherapy, Adoptive/methods ; Lentivirus/genetics ; Lymphocyte Activation ; Male ; Mice ; Mice, Inbred NOD ; Protein Engineering ; Receptor, ErbB-2/immunology ; Receptors, Antigen/genetics/*immunology ; Receptors, Chimeric Antigen/genetics/*immunology ; Recombinant Proteins/*immunology ; Single-Domain Antibodies ; T-Cell Antigen Receptor Specificity/genetics/*immunology ; T-Lymphocytes/*immunology ; T-Lymphocytes, Cytotoxic/immunology ; Vision, Ocular ; Xenograft Model Antitumor Assays ; }, abstract = {Engineering T cells with chimeric antigen receptors (CARs) is an effective method for directing T cells to attack tumors, but may cause adverse side effects such as the potentially lethal cytokine release syndrome. Here the authors show that the T cell antigen coupler (TAC), a chimeric receptor that co-opts the endogenous TCR, induces more efficient anti-tumor responses and reduced toxicity when compared with past-generation CARs. TAC-engineered T cells induce robust and antigen-specific cytokine production and cytotoxicity in vitro, and strong anti-tumor activity in a variety of xenograft models including solid and liquid tumors. In a solid tumor model, TAC-T cells outperform CD28-based CAR-T cells with increased anti-tumor efficacy, reduced toxicity, and faster tumor infiltration. Intratumoral TAC-T cells are enriched for Ki-67+ CD8+ T cells, demonstrating local expansion. These results indicate that TAC-T cells may have a superior therapeutic index relative to CAR-T cells.}, }
@article {pmid30064273, year = {2020}, author = {Morgunov, LY and Denisova, IA and Rozhkova, TI and Stakhovskaya, LV and Skvortsova, VI}, title = {Hypogonadism and its treatment following ischaemic stroke in men with type 2 diabetes mellitus.}, journal = {The aging male : the official journal of the International Society for the Study of the Aging Male}, volume = {23}, number = {1}, pages = {71-80}, doi = {10.1080/13685538.2018.1487932}, pmid = {30064273}, issn = {1473-0790}, mesh = {Aged ; Biomarkers/blood ; Diabetes Mellitus, Type 2/*complications ; Gonadal Steroid Hormones/blood ; Humans ; Hypogonadism/*drug therapy/epidemiology/*etiology ; Male ; Middle Aged ; Russia/epidemiology ; Stroke/*complications ; Testosterone/*analogs &amp; derivatives/therapeutic use ; }, abstract = {Premature mortality in Russia is a major socio-economic problem, especially from acute cerebrovascular diseases which constitute 21.4% of the total mortality and is a considerable contributor to chronic disability. Risk of vascular catastrophe is higher in males than females, thought, in part, due to anti-atherosclerotic effects of oestrogens in females whilst an associated age-related deficiency of testosterone is observed in men. Clinical symptoms such as high blood pressure, changes in lipid profile, insulin resistance, obesity, and blood coagulation factors often accompany declining testosterone in males and reduced total testosterone is considered a cardiovascular risk factor. In the present study, the prevalence of hypogonadism in men who had suffered ischaemic stroke was evaluated along with the efficacy of testosterone undecanoate injections (TU) in patients with testosterone deficiency and type-2 diabetes (T2DM) in the acute phase of hemispheric ischaemic stroke. Hypogonadism was present in 66.3% of patients with ischaemic stroke, 50% with T2DM, and 26.3% without T2DM, respectively. TU treatment, at both the 2 and 5-year observation points, demonstrated significant improvements in biochemical, physical, and mental parameters. This supports that testosterone deficiency is a contributing factor in ischaemic events and that long-term testosterone therapy could play an important role in patient recovery.}, }
@article {pmid30022013, year = {2018}, author = {Dolgova, O and Lao, O}, title = {Evolutionary and Medical Consequences of Archaic Introgression into Modern Human Genomes.}, journal = {Genes}, volume = {9}, number = {7}, pages = {}, pmid = {30022013}, issn = {2073-4425}, abstract = {The demographic history of anatomically modern humans (AMH) involves multiple migration events, population extinctions and genetic adaptations. As genome-wide data from complete genome sequencing becomes increasingly abundant and available even from extinct hominins, new insights of the evolutionary history of our species are discovered. It is currently known that AMH interbred with archaic hominins once they left the African continent. Current non-African human genomes carry fragments of archaic origin. This review focuses on the fitness consequences of archaic interbreeding in current human populations. We discuss new insights and challenges that researchers face when interpreting the potential impact of introgression on fitness and testing hypotheses about the role of selection within the context of health and disease.}, }
@article {pmid30010009, year = {2019}, author = {Denisova, K}, title = {Neurobiology, not artifacts: Challenges and guidelines for imaging the high risk infant.}, journal = {NeuroImage}, volume = {185}, number = {}, pages = {624-640}, doi = {10.1016/j.neuroimage.2018.07.023}, pmid = {30010009}, issn = {1095-9572}, mesh = {*Artifacts ; Autism Spectrum Disorder/*diagnostic imaging ; Female ; *Head Movements ; Humans ; Infant ; Magnetic Resonance Imaging/methods ; Male ; Neurobiology ; Neuroimaging/methods ; }, abstract = {The search for the brain-basis of atypical development in human infants is challenging because the process of imaging and the generation of the MR signal itself relies on assumptions that reflect biophysical properties of the brain tissue. These assumptions are not inviolate, have been questioned by recent empirical evidence from high risk infant-sibling studies, and to date remain largely underexamined at the between-group level. In particular, I consider recent work showing that infants at High vs. Low familial risk (HR vs. LR, respectively) for developing Autism Spectrum Disorders (ASD) have atypical patterns of head movements during an MR scan that are functionally important-they are linked to future learning trajectories in toddlerhood. Addressing head movement issues in neuroimaging analyses in infant research as well as understanding the causes of these movements from a developmental perspective requires acknowledging the complexity of this endeavor. For example, head movement signatures in infants can interact with experimental task conditions (such as listening to language compared to sleeping), autism risk, and age. How can new knowledge about newborns' individual, subject-specific behavioral differences which may impact MR signal acquisition and statistical inference ignite critical thinking for the field of infant brain imaging across the spectrum of typical and atypical development? Early behavioral differences between HR and LR infant cohorts that are often examples of "artifactual" confounds in MR work provide insight into nascent neurobiological differences, including biophysical tissue properties and hemodynamic response variability, in these and related populations at risk for atypical development. Are these neurobiological drivers of atypical development? This work identifies important knowledge gaps and suggests guidelines at the leading edge of baby imaging science to transform our understanding of atypical brain development in humans. The precise study of the neurobiological underpinnings of atypical development in humans calls for approaches including quantitative MRI (qMRI) pulse sequences, multi-modal imaging (including DTI, MRS, as well as MEG), and infant-specific HRF shapes when modeling BOLD signal.}, }
@article {pmid30004998, year = {2021}, author = {Raevis, JJ and Denisova, K and Mechel, E and Shrier, EM}, title = {MOSAICISM AS A PROPOSED MECHANISM FOR ASYMMETRIC RETINAL TESSELLATIONS.}, journal = {Retinal cases &amp; brief reports}, volume = {15}, number = {3}, pages = {214-217}, doi = {10.1097/ICB.0000000000000770}, pmid = {30004998}, issn = {1937-1578}, abstract = {BACKGROUND/PURPOSE: Report a case of markedly asymmetric retinal tessellations and propose mosaicism as a mechanism.<br><br>METHODS AND RESULTS: A 59-year-old pseudophakic woman presented with uncorrected 20/20 vision and was found to have markedly different retinal tessellation appearances in both eyes. The axial lengths were 25.66 mm and 25.88 mm in the right and left eyes, respectively, and no significant asymmetrical choroidal thinning was seen on optical coherence tomography or optical coherence tomography angiography. Fluorescein angiogram showed significant hyperfluorescence, representing the underlying choroid, which correlated with the tessellation patterns in the left eye. She had no other ocular or systemic findings such as stripes or whorled skin.<br><br>CONCLUSION: This is the first reported case of markedly asymmetric retinal tessellation patterns that are not due to asymmetric axial myopia or choroidal thinning. We propose that mosaicism is a possible mechanism causing this finding.}, }
@article {pmid29995885, year = {2018}, author = {Zhao, G and Walsh, K and Long, J and Gui, W and Denisova, K}, title = {Reduced structural complexity of the right cerebellar cortex in male children with autism spectrum disorder.}, journal = {PloS one}, volume = {13}, number = {7}, pages = {e0196964}, pmid = {29995885}, issn = {1932-6203}, mesh = {Autism Spectrum Disorder/*diagnostic imaging/pathology/physiopathology ; Case-Control Studies ; Cerebellar Cortex/*diagnostic imaging/pathology/physiopathology ; Child ; Child Development/physiology ; Cognition/*physiology ; Fractals ; Gray Matter/*diagnostic imaging/pathology/physiopathology ; Humans ; Magnetic Resonance Imaging ; Male ; Sex Factors ; }, abstract = {The cerebellum contains 80% of all neurons in the human brain and contributes prominently to implicit learning and predictive processing across motor, sensory, and cognitive domains. As morphological features of the cerebellum in atypically developing individuals remain unexplored in-vivo, this is the first study to use high-resolution 3D fractal analysis to estimate fractal dimension (FD), a measure of structural complexity of an object, of the left and right cerebellar cortex (automatically segmented from Magnetic Resonance Images using FreeSurfer), in male children with Autism Spectrum Disorders (ASD) (N = 20; mean age: 8.8 years old, range: 7.13-10.27) and sex, age, verbal-IQ, and cerebellar volume-matched typically developing (TD) boys (N = 18; mean age: 8.9 years old, range: 6.47-10.52). We focus on an age range within the 'middle and late childhood' period of brain development, between 6 and 12 years. A Mann-Whitney U test revealed a significant reduction in the FD of the right cerebellar cortex in ASD relative to TD boys (P = 0.0063, Bonferroni-corrected), indicating flatter and less regular surface protrusions in ASD relative to TD males. Consistent with the prediction that the cerebellum participates in implicit learning, those ASD boys with a higher (vs. lower) PIQ>VIQ difference showed higher, more normative complexity values, closer to TD children, providing new insight on our understanding of the neurological basis of differences in verbal and performance cognitive abilities that often characterize individuals with ASD.}, }
@article {pmid29948329, year = {2018}, author = {Malyarchuk, B and Derenko, M and Denisova, G and Litvinov, A and Rogalla, U and Skonieczna, K and Grzybowski, T and Pentelényi, K and Guba, Z and Zeke, T and Molnár, MJ}, title = {Whole mitochondrial genome diversity in two Hungarian populations.}, journal = {Molecular genetics and genomics : MGG}, volume = {293}, number = {5}, pages = {1255-1263}, pmid = {29948329}, issn = {1617-4623}, support = {16-34-00014//Russian Foundation for Basic Research/ ; ICRG-117//the Wenner-Gren Foundation/ ; }, mesh = {DNA, Mitochondrial/*genetics ; Ethnic Groups/*genetics ; *Genetic Variation ; *Genetics, Population ; *Genome, Mitochondrial ; *Haplotypes ; Humans ; Hungary ; Phylogeography ; }, abstract = {Complete mitochondrial genomics is an effective tool for studying the demographic history of human populations, but there is still a deficit of mitogenomic data in European populations. In this paper, we present results of study of variability of 80 complete mitochondrial genomes in two Hungarian populations from eastern part of Hungary (Szeged and Debrecen areas). The genetic diversity of Hungarian mitogenomes is remarkably high, reaching 99.9% in a combined sample. According to the analysis of molecular variance (AMOVA), European populations showed a low, but statistically significant level of between-population differentiation (Fst = 0.61%, p = 0), and two Hungarian populations demonstrate lack of between-population differences. Phylogeographic analysis allowed us to identify 71 different mtDNA sub-clades in Hungarians, sixteen of which are novel. Analysis of ancestry-informative mtDNA sub-clades revealed a complex genetic structure associated with the genetic impact of populations from different parts of Eurasia, though the contribution from European populations is the most pronounced. At least 8% of ancestry-informative haplotypes found in Hungarians demonstrate similarity with East and West Slavic populations (sub-clades H1c23a, H2a1c1, J2b1a6, T2b25a1, U4a2e, K1c1j, and I1a1c), while the influence of Siberian populations is not so noticeable (sub-clades A12a, C4a1a, and probably U4b1a4).}, }
@article {pmid29927400, year = {2018}, author = {Rzaev, DA and Denisova, NP and Moisak, GI and Rogov, DY and Kulikova, EV}, title = {[Experience of the use of gasserian ganglion balloon compression in patients with trigeminal neuralgia associated with multiple sclerosis].}, journal = {Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova}, volume = {118}, number = {5}, pages = {30-35}, doi = {10.17116/jnevro20181185130}, pmid = {29927400}, issn = {1997-7298}, mesh = {Aged ; Catheterization ; Female ; Humans ; Male ; Middle Aged ; *Multiple Sclerosis ; Recurrence ; Treatment Outcome ; Trigeminal Ganglion ; *Trigeminal Neuralgia ; }, abstract = {AIM: To evaluate the efficacy of gasserian ganglion balloon compression in patients with trigeminal neuralgia associated with multiple sclerosis (MS).<br><br>MATERIAL AND METHODS: Eight patients (3 men, 5 women), aged from 46 to 66 years (mean age 55 years), with trigeminal neuralgia associated with MS underwent surgery. An average duration of the pain syndrome was 8,4 years. Six patients had previous surgeries due to facial pain. Percutaneous balloon compression of gasserian ganglion was performed to all patients. Follow up period was from 2 to 24 months.<br><br>RESULTS: Six patients (75%) reported 100% of pain relief right after the surgery, 2 patients (25%) reported a significant decrease of pain (2-3 points on VAS). Pain recurrence occurred in 3 patients: in 4 months, in 12 months and in 6 months. All of them were operated repeatedly. After the surgery, hypoesthesia on the side of surgery was observed in all patients with a trend towards regression. There was no keratopathy or any complications.<br><br>CONCLUSION: Percutaneous balloon compression of gasserian ganglion is an effective and minimally invasive method which can be performed repeatedly in patients with trigeminal neuralgia associated with MS.}, }
@article {pmid29914355, year = {2018}, author = {Cserhati, MF and Mooter, ME and Peterson, L and Wicks, B and Xiao, P and Pauley, M and Guda, C}, title = {Motifome comparison between modern human, Neanderthal and Denisovan.}, journal = {BMC genomics}, volume = {19}, number = {1}, pages = {472}, pmid = {29914355}, issn = {1471-2164}, support = {P20 GM103427/GM/NIGMS NIH HHS/United States ; P30 CA036727/CA/NCI NIH HHS/United States ; 2P20GM103427//Nebraska INBRE/ ; 5P30CA036727//CCSG award/ ; }, mesh = {Animals ; Endogenous Retroviruses/genetics ; *Fossils ; *Genome ; Humans ; Neanderthals/*genetics ; Nucleotide Motifs/*genetics ; Phenotype ; Promoter Regions, Genetic ; Trans-Activators ; }, abstract = {BACKGROUND: The availability of the genomes of two archaic humans, Neanderthal and Denisovan, and that of modern humans provides researchers an opportunity to investigate genetic differences between these three subspecies on a genome-wide scale. Here we describe an algorithm that predicts statistically significant motifs based on the difference between a given motif's actual and expected distributions. The algorithm was previously applied to plants but was modified for this work.<br><br>RESULTS: The result of applying the algorithm to the human, Neanderthal, and Denisovan genomes is a catalog of potential regulatory motifs in these three human subspecies. We examined the distributions of these motifs in genetic elements including human retroviruses, human accelerated regions, and human accelerated conserved noncoding sequences regions. Differences in these distributions could be the origin of differences in phenotype between the three subspecies. Twenty significant motifs common to all three genomes were found; thirty-three were found in endogenous retroviruses in Neanderthal and Denisovan. Ten of these motifs mapped to the 22 bp core of MiR-1304. The core of this genetic element regulates the ENAM and AMTN genes, which take part in odontogenesis and whose 3' UTRs contained significant motifs. The introns of 20 genes were found to contain a large number of significant motifs, which were also overrepresented in 49 human accelerated regions. These genes include NAV2, SorCS2, TRAPPC9, GRID1, PRDM16, CAMTA1, and ASIC which are all involved in neuroregulation. Further analysis of these genes using the GO database indicates that many are associated with neurodevelopment. Also, varying numbers of significant motifs were found to occur in regions of the Neanderthal and Denisovan genomes that are missing from the human genome, suggesting further functional differences between modern and archaic humans.<br><br>CONCLUSION: Although Neanderthal and Denisovan are now extinct, detailed examination of elements from their genomes can shed light on possible phenotypic and cognitive differences between these two archaic human subspecies and modern humans. Genetic similarities and differences between these three subspecies and other fossil hominids would also be of interest.}, }
@article {pmid29747567, year = {2018}, author = {Banerjee, N and Polushina, T and Bettella, F and Giddaluru, S and Steen, VM and Andreassen, OA and Le Hellard, S}, title = {Recently evolved human-specific methylated regions are enriched in schizophrenia signals.}, journal = {BMC evolutionary biology}, volume = {18}, number = {1}, pages = {63}, pmid = {29747567}, issn = {1471-2148}, support = {#2 T23273//Norges Forskningsråd/International ; SKGJ-MED-008//KG Jebsen Foundation/International ; }, mesh = {Adult ; Bipolar Disorder/genetics ; Body Height/genetics ; Body Mass Index ; DNA Methylation/*genetics ; *Evolution, Molecular ; Female ; Genetic Markers ; Genome-Wide Association Study ; Genotype ; Humans ; Major Histocompatibility Complex/genetics ; Male ; Molecular Sequence Annotation ; Multifactorial Inheritance ; Phenotype ; Polymorphism, Single Nucleotide/genetics ; Schizophrenia/*genetics ; }, abstract = {BACKGROUND: One explanation for the persistence of schizophrenia despite the reduced fertility of patients is that it is a by-product of recent human evolution. This hypothesis is supported by evidence suggesting that recently-evolved genomic regions in humans are involved in the genetic risk for schizophrenia. Using summary statistics from genome-wide association studies (GWAS) of schizophrenia and 11 other phenotypes, we tested for enrichment of association with GWAS traits in regions that have undergone methylation changes in the human lineage compared to Neanderthals and Denisovans, i.e. human-specific differentially methylated regions (DMRs). We used analytical tools that evaluate polygenic enrichment of a subset of genomic variants against all variants.<br><br>RESULTS: Schizophrenia was the only trait in which DMR SNPs showed clear enrichment of association that passed the genome-wide significance threshold. The enrichment was not observed for Neanderthal or Denisovan DMRs. The enrichment seen in human DMRs is comparable to that for genomic regions tagged by Neanderthal Selective Sweep markers, and stronger than that for Human Accelerated Regions. The enrichment survives multiple testing performed through permutation (n = 10,000) and bootstrapping (n = 5000) in INRICH (p < 0.01). Some enrichment of association with height was observed at the gene level.<br><br>CONCLUSIONS: Regions where DNA methylation modifications have changed during recent human evolution show enrichment of association with schizophrenia and possibly with height. Our study further supports the hypothesis that genetic variants conferring risk of schizophrenia co-occur in genomic regions that have changed as the human species evolved. Since methylation is an epigenetic mark, potentially mediated by environmental changes, our results also suggest that interaction with the environment might have contributed to that association.}, }
@article {pmid29739306, year = {2018}, author = {Akkuratov, EE and Gelfand, MS and Khrameeva, EE}, title = {Neanderthal and Denisovan ancestry in Papuans: A functional study.}, journal = {Journal of bioinformatics and computational biology}, volume = {16}, number = {2}, pages = {1840011}, doi = {10.1142/S0219720018400115}, pmid = {29739306}, issn = {1757-6334}, mesh = {African Continental Ancestry Group/genetics ; Alleles ; Animals ; Bone Remodeling/genetics ; Cluster Analysis ; Genetics, Population ; *Genome, Human ; Hominidae/*genetics ; Humans ; Multigene Family ; Neanderthals/genetics ; Papua New Guinea ; Polymorphism, Single Nucleotide ; Transcription Factors/genetics ; }, abstract = {Sequencing of complete nuclear genomes of Neanderthal and Denisovan stimulated studies about their relationship with modern humans demonstrating, in particular, that DNA alleles from both Neanderthal and Denisovan genomes are present in genomes of modern humans. The Papuan genome is a unique object because it contains both Neanderthal and Denisovan alleles. Here, we have shown that the Papuan genomes contain different gene functional groups inherited from each of the ancient people. The Papuan genomes demonstrate a relative prevalence of Neanderthal alleles in genes responsible for the regulation of transcription and neurogenesis. The enrichment of specific functional groups with Denisovan alleles is less pronounced; these groups are responsible for bone and tissue remodeling. This analysis shows that introgression of alleles from Neanderthals and Denisovans to Papuans occurred independently and retention of these alleles may carry specific adaptive advantages.}, }
@article {pmid29688213, year = {2018}, author = {Shkliaev, AE and Denisova, NI and Kulikov, IV}, title = {[Splenic artery aneurysm masked by postcholecystectomy syndrome].}, journal = {Angiologiia i sosudistaia khirurgiia = Angiology and vascular surgery}, volume = {24}, number = {1}, pages = {175-178}, pmid = {29688213}, issn = {1027-6661}, mesh = {*Aneurysm/diagnosis/physiopathology/surgery ; Angiography/methods ; Blood Vessel Prosthesis Implantation/*methods ; Diagnosis, Differential ; Endovascular Procedures/*methods ; Female ; Humans ; Middle Aged ; Postcholecystectomy Syndrome/*diagnosis ; *Splenic Artery/diagnostic imaging/pathology ; Tomography, Spiral Computed/methods ; Treatment Outcome ; }, abstract = {Visceral artery aneurysms appear to belong to uncommon and potentially lethal vascular diseases. They are usually revealed accidentally during an ultrasonographic examination, magnetic resonance imaging, or computed tomography. Described in the article is a clinical case report concerning a sacciform aneurysm of the splenic artery, detected in a 53-year-old woman presenting with postcholecystectomy syndrome and followed up for abdominalgia by therapeutists and gastroenterologists. Timely performed radiodiagnosis (including multispiral computed tomography and angiography of the abdominal vessels) made it possible not only to detect the aneurysm, having thus verified the volumetric formation previously found on ultrasonographic examination, but to take adequate measures aimed at preventing rupture of the aneurysm and consisting in endovascular occlusion of the aneurysmatic cavity with metal spirals. Lack of complete clarity in the understanding of the mechanisms of the origin of and no distinctly defined therapeutic-and-diagnostic algorithm for visceral artery aneurysms dictate the necessity to continue collecting and generalizing clinical case reports regarding this rarely encountered vascular pathology.}, }
@article {pmid29667630, year = {2018}, author = {Bogomolov, DV and Fetisov, VA and Denisova, OP and Zbrueva, YV and Semenov, GG}, title = {[The principal and auxiliary immunohistochemical markers of intravital mechanical strangulation asphyxia].}, journal = {Sudebno-meditsinskaia ekspertiza}, volume = {61}, number = {2}, pages = {11-13}, doi = {10.17116/sudmed201861211-13}, pmid = {29667630}, issn = {0039-4521}, mesh = {Adolescent ; Adult ; *Asphyxia/etiology/pathology ; Biomarkers/analysis ; *Cause of Death ; Child ; Diagnosis, Differential ; Fibrinogen/immunology ; Forensic Pathology/methods ; Humans ; Immunohistochemistry/*methods ; *Lung/immunology/pathology ; *Neck Injuries/immunology/pathology ; Proto-Oncogene Proteins c-kit/*analysis ; }, abstract = {The objective of the present study was the evaluation of the auxiliary methods for the diagnostics of the intravital formation of the constriction marks; the secondary objective was to determine the pace at which the death and asphyxia occur.<br><br>MATERIAL AND METHODS: The materials on which the study was based included 17 cases of mechanical strangulation asphyxia involving 13 men and 4 women at the age from 8 to 28 years. All cases of hanging were associated with different blood alcohol levels. Their characteristic feature was the formation of the obliquely ascending constriction marks. The group of comparison was comprised of three cases of death by drowning and one case of manual strangulation. The control group consisted of 10 patients who died from the acute form of coronary heart disease and 5 cases of death from traumatic shock. All the corpses were examined with the use of the traditional methods within the first 24 hours after death. The special laboratory studies were performed by means of the standard histological and immunohistochemical methods with the use of the polyclonal antibodies against total cytokeratin, fibrinogen, immunoglobulin-lambda, fibronectin, and CD-117 antigen. The histological preparations were stained by the method of Spielmeyer and with toluidine blue.<br><br>RESULTS: The results of the study give evidence of the possibility of diagnostics of mechanical strangulation asphyxia making use of the reaction with anti-fibrinogen antibodies in the stromal tissue of the constriction mark even in the absence of other intravital signs of death. Such diagnostics is also possible with the use of the CD-117 antigen in the pulmonary tissue. The expression of this antigen is characteristic of the cases of alveolar hypoxia. The possibility of application of other markers for the differential diagnostics of mechanical strangulation asphyxia from other causes of death is discussed.}, }
@article {pmid29659494, year = {2018}, author = {Khabarova, EA and Denisova, NP and Dmitriev, AB and Slavin, KV and Verhagen Metman, L}, title = {Deep Brain Stimulation of the Subthalamic Nucleus in Patients with Parkinson Disease with Prior Pallidotomy or Thalamotomy.}, journal = {Brain sciences}, volume = {8}, number = {4}, pages = {}, pmid = {29659494}, issn = {2076-3425}, abstract = {Objective. To evaluate the efficacy of deep brain stimulation of the subthalamic nucleus (STN DBS) in patients with Parkinson disease (PD) who previously underwent lesioning of the basal ganglia. Material and methods. The study included 22 patients who underwent STN DBS. Eleven patients had undergone prior unilateral pallidotomy (n = 6) or VL/VIM thalamotomy (n = 5) while the other 11 patients had not. The primary outcome was the change from baseline in the motor subscore of the Unified Parkinson Disease Rating Scale (UPDRS-III) 12 months after STN DBS. Secondary outcomes included change in motor response complications (UPDRS-IV) and change in levodopa equivalent daily dose (LEDD). Results. In the group with prior lesioning UPDRS-III improved by 45%, from 51.5 &amp;plusmn; 9.0% (range, 35&amp;ndash;65) to 26.5 &amp;plusmn; 8.4 (range, 21&amp;ndash;50) (p < 0.01) and UPDRS-IV by 75%, from 8.0 &amp;plusmn; 2.01 (range, 5&amp;ndash;11) to 2.1 &amp;plusmn; 0.74 (range, 1&amp;ndash;3) (p < 0.01). In the group without prior lesioning UPDRS-III improved by 61%, from 74.2% &amp;plusmn; 7.32 (range, 63&amp;ndash;82) to 29.3 &amp;plusmn; 5.99 (range, 20&amp;ndash;42) (p < 0.01) and UPDRS-IV by 77%, from 9.1 &amp;plusmn; 2.46 (range, 5&amp;ndash;12) to 2.0 &amp;plusmn; 1.1 (range, 1&amp;ndash;4) (p < 0.01). Comparing the two groups (with and without lesioning) no significant differences were found either in UPDRS-III (p > 0.05) or UPDRS-IV scores (p > 0.05) at 12 months post-DBS. The LEDD was reduced by 51.4%, from 1008.2 &amp;plusmn; 346.4 to 490.0 &amp;plusmn; 194.3 in those with prior surgery (p < 0.01) and by 55.0%, from 963.4 &amp;plusmn; 96.2 to 433.3 &amp;plusmn; 160.2 in those without (p < 0.01).UPDRS-III improved by 51.8%, from 53.7 &amp;plusmn; 4.6 (range, 50&amp;ndash;62) to 25.0 &amp;plusmn; 3.8 (range, 21&amp;ndash;31) in those with prior pallidotomy (p < 0.01), and by 37.5%, from 48.8 &amp;plusmn; 12.6 (range, 35&amp;ndash;65) to 29.8 &amp;plusmn; 13.6 (range, 22&amp;ndash;50) in those with prior thalamotomy (p < 0.01). This numerical difference in improvement was not statistically significant (p > 0.05). Conclusion. Our comparative study indicates that bilateral STN DBS is effective and can be used in patients with Parkinson disease with prior unilateral stereotactic destructive operations on subcortical structures. The results in our patient cohort are generally consistent with previously published reports of smaller series from multiple centers worldwide.}, }
@article {pmid29658973, year = {2018}, author = {Viscardi, LH and Paixão-Côrtes, VR and Comas, D and Salzano, FM and Rovaris, D and Bau, CD and Amorim, CEG and Bortolini, MC}, title = {Searching for ancient balanced polymorphisms shared between Neanderthals and Modern Humans.}, journal = {Genetics and molecular biology}, volume = {41}, number = {1}, pages = {67-81}, pmid = {29658973}, issn = {1415-4757}, abstract = {Hominin evolution is characterized by adaptive solutions often rooted in behavioral and cognitive changes. If balancing selection had an important and long-lasting impact on the evolution of these traits, it can be hypothesized that genes associated with them should carry an excess of shared polymorphisms (trans- SNPs) across recent Homo species. In this study, we investigate the role of balancing selection in human evolution using available exomes from modern (Homo sapiens) and archaic humans (H. neanderthalensis and Denisovan) for an excess of trans-SNP in two gene sets: one associated with the immune system (IMMS) and another one with behavioral system (BEHS). We identified a significant excess of trans-SNPs in IMMS (N=547), of which six of these located within genes previously associated with schizophrenia. No excess of trans-SNPs was found in BEHS, but five genes in this system harbor potential signals for balancing selection and are associated with psychiatric or neurodevelopmental disorders. Our approach evidenced recent Homo trans-SNPs that have been previously implicated in psychiatric diseases such as schizophrenia, suggesting that a genetic repertoire common to the immune and behavioral systems could have been maintained by balancing selection starting before the split between archaic and modern humans.}, }
@article {pmid29608725, year = {2018}, author = {Zehra, R and Abbasi, AA}, title = {Homo sapiens-Specific Binding Site Variants within Brain Exclusive Enhancers Are Subject to Accelerated Divergence across Human Population.}, journal = {Genome biology and evolution}, volume = {10}, number = {3}, pages = {956-966}, pmid = {29608725}, issn = {1759-6653}, mesh = {Animals ; Binding Sites/genetics ; Brain/metabolism ; Enhancer Elements, Genetic/*genetics ; *Evolution, Molecular ; Humans ; Primates/*genetics ; Species Specificity ; Transcription Factors/genetics ; }, abstract = {Empirical assessments of human accelerated noncoding DNA frgaments have delineated presence of many cis-regulatory elements. Enhancers make up an important category of such accelerated cis-regulatory elements that efficiently control the spatiotemporal expression of many developmental genes. Establishing plausible reasons for accelerated enhancer sequence divergence in Homo sapiens has been termed significant in various previously published studies. This acceleration by including closely related primates and archaic human data has the potential to open up evolutionary avenues for deducing present-day brain structure. This study relied on empirically confirmed brain exclusive enhancers to avoid any misjudgments about their regulatory status and categorized among them a subset of enhancers with an exceptionally accelerated rate of lineage specific divergence in humans. In this assorted set, 13 distinct transcription factor binding sites were located that possessed unique existence in humans. Three of 13 such sites belonging to transcription factors SOX2, RUNX1/3, and FOS/JUND possessed single nucleotide variants that made them unique to H. sapiens upon comparisons with Neandertal and Denisovan orthologous sequences. These variants modifying the binding sites in modern human lineage were further substantiated as single nucleotide polymorphisms via exploiting 1000 Genomes Project Phase3 data. Long range haplotype based tests laid out evidence of positive selection to be governing in African population on two of the modern human motif modifying alleles with strongest results for SOX2 binding site. In sum, our study acknowledges acceleration in noncoding regulatory landscape of the genome and highlights functional parts within it to have undergone accelerated divergence in present-day human population.}, }
@article {pmid29570998, year = {2018}, author = {Vernot, B and Pääbo, S}, title = {The Predecessors Within . . .}, journal = {Cell}, volume = {173}, number = {1}, pages = {6-7}, doi = {10.1016/j.cell.2018.03.023}, pmid = {29570998}, issn = {1097-4172}, mesh = {Asia ; *Family ; Humans ; }, abstract = {By examining the genomes of present-day people from Asia, researchers show that modern humans met and interbred with Denisovans, distant relatives to Neanderthals, on at least two occasions. As a result, people today carry DNA from two different Denisovan populations.}, }
@article {pmid29551270, year = {2018}, author = {Browning, SR and Browning, BL and Zhou, Y and Tucci, S and Akey, JM}, title = {Analysis of Human Sequence Data Reveals Two Pulses of Archaic Denisovan Admixture.}, journal = {Cell}, volume = {173}, number = {1}, pages = {53-61.e9}, pmid = {29551270}, issn = {1097-4172}, support = {R01 GM110068/GM/NIGMS NIH HHS/United States ; }, mesh = {Animals ; Asian Continental Ancestry Group/genetics ; *Genome, Human ; Humans ; Neanderthals/genetics ; Selection, Genetic ; Whole Exome Sequencing ; }, abstract = {Anatomically modern humans interbred with Neanderthals and with a related archaic population known as Denisovans. Genomes of several Neanderthals and one Denisovan have been sequenced, and these reference genomes have been used to detect introgressed genetic material in present-day human genomes. Segments of introgression also can be detected without use of reference genomes, and doing so can be advantageous for finding introgressed segments that are less closely related to the sequenced archaic genomes. We apply a new reference-free method for detecting archaic introgression to 5,639 whole-genome sequences from Eurasia and Oceania. We find Denisovan ancestry in populations from East and South Asia and Papuans. Denisovan ancestry comprises two components with differing similarity to the sequenced Altai Denisovan individual. This indicates that at least two distinct instances of Denisovan admixture into modern humans occurred, involving Denisovan populations that had different levels of relatedness to the sequenced Altai Denisovan. VIDEO ABSTRACT.}, }
@article {pmid29477182, year = {2018}, author = {Warren, KA and Ritzman, TB and Humphreys, RA and Percival, CJ and Hallgrímsson, B and Ackermann, RR}, title = {Craniomandibular form and body size variation of first generation mouse hybrids: A model for hominin hybridization.}, journal = {Journal of human evolution}, volume = {116}, number = {}, pages = {57-74}, pmid = {29477182}, issn = {1095-8606}, support = {R01 DE019638/DE/NIDCR NIH HHS/United States ; }, mesh = {Animals ; Biological Evolution ; Body Size/genetics ; Fossils/anatomy &amp; histology ; Hominidae/*anatomy &amp; histology/genetics/*physiology ; *Hybridization, Genetic ; Mandible/anatomy &amp; histology ; Mice/*anatomy &amp; histology/genetics/*physiology ; *Models, Animal ; Phenotype ; Skull/anatomy &amp; histology ; }, abstract = {Hybridization occurs in a number of mammalian lineages, including among primate taxa. Analyses of ancient genomes have shown that hybridization between our lineage and other archaic hominins in Eurasia occurred numerous times in the past. However, we still have limited empirical data on what a hybrid skeleton looks like, or how to spot patterns of hybridization among fossils for which there are no genetic data. Here we use experimental mouse models to supplement previous studies of primates. We characterize size and shape variation in the cranium and mandible of three wild-derived inbred mouse strains and their first generation (F1) hybrids. The three parent taxa in our analysis represent lineages that diverged over approximately the same period as the human/Neanderthal/Denisovan lineages and their hybrids are variably successful in the wild. Comparisons of body size, as quantified by long bone measurements, are also presented to determine whether the identified phenotypic effects of hybridization are localized to the cranium or represent overall body size changes. The results indicate that hybrid cranial and mandibular sizes, as well as limb length, exceed that of the parent taxa in all cases. All three F1 hybrid crosses display similar patterns of size and form variation. These results are generally consistent with earlier studies on primates and other mammals, suggesting that the effects of hybridization may be similar across very different scenarios of hybridization, including different levels of hybrid fitness. This paper serves to supplement previous studies aimed at identifying F1 hybrids in the fossil record and to introduce further research that will explore hybrid morphologies using mice as a proxy for better understanding hybridization in the hominin fossil record.}, }
@article {pmid29445326, year = {2018}, author = {Leacock, S and Syed, P and James, VM and Bode, A and Kawakami, K and Keramidas, A and Suster, M and Lynch, JW and Harvey, RJ}, title = {Structure/Function Studies of the α4 Subunit Reveal Evolutionary Loss of a GlyR Subtype Involved in Startle and Escape Responses.}, journal = {Frontiers in molecular neuroscience}, volume = {11}, number = {}, pages = {23}, pmid = {29445326}, issn = {1662-5099}, support = {MR/J004049/1//Medical Research Council/United Kingdom ; }, abstract = {Inhibitory glycine receptors (GlyRs) are pentameric ligand-gated anion channels with major roles in startle disease/hyperekplexia (GlyR α1), cortical neuronal migration/autism spectrum disorder (GlyR α2), and inflammatory pain sensitization/rhythmic breathing (GlyR α3). However, the role of the GlyR α4 subunit has remained enigmatic, because the corresponding human gene (GLRA4) is thought to be a pseudogene due to an in-frame stop codon at position 390 within the fourth membrane-spanning domain (M4). Despite this, a recent genetic study has implicated GLRA4 in intellectual disability, behavioral problems and craniofacial anomalies. Analyzing data from sequenced genomes, we found that GlyR α4 subunit genes are predicted to be intact and functional in the majority of vertebrate species-with the exception of humans. Cloning of human GlyR α4 cDNAs excluded alternative splicing and RNA editing as mechanisms for restoring a full-length GlyR α4 subunit. Moreover, artificial restoration of the missing conserved arginine (R390) in the human cDNA was not sufficient to restore GlyR α4 function. Further bioinformatic and mutagenesis analysis revealed an additional damaging substitution at K59 that ablates human GlyR α4 function, which is not present in other vertebrate GlyR α4 sequences. The substitutions K59 and X390 were also present in the genome of an ancient Denisovan individual, indicating that GLRA4 has been a pseudogene for at least 30,000-50,000 years. In artificial synapses, we found that both mouse and gorilla α4β GlyRs mediate synaptic currents with unusually slow decay kinetics. Lastly, to gain insights into the biological role of GlyR α4 function, we studied the duplicated genes glra4a and glra4b in zebrafish. While glra4b expression is restricted to the retina, using a novel tol2-GAL4FF gene trap line (SAIGFF16B), we found that the zebrafish GlyR α4a subunit gene (glra4a) is strongly expressed in spinal cord and hindbrain commissural neurones. Using gene knockdown and a dominant-negative GlyR α4aR278Q mutant, we found that GlyR α4a contributes to touch-evoked escape behaviors in zebrafish. Thus, although GlyR α4 is unlikely to be involved in human startle responses or disease states, this subtype may contribute to escape behaviors in other organisms.}, }
@article {pmid29428968, year = {2018}, author = {Jiang, L and Peng, J and Huang, M and Liu, J and Wang, L and Ma, Q and Zhao, H and Yang, X and Ji, A and Li, C}, title = {Differentiation analysis for estimating individual ancestry from the Tibetan Plateau by an archaic altitude adaptation EPAS1 haplotype among East Asian populations.}, journal = {International journal of legal medicine}, volume = {132}, number = {6}, pages = {1527-1535}, pmid = {29428968}, issn = {1437-1596}, support = {2017YFC0803501//National Key Research and Development Program of China/ ; 2016JB039//basic research project/ ; 2017JB027//basic research project/ ; }, mesh = {Adaptation, Physiological ; Altitude ; Asian Continental Ancestry Group/*genetics ; Basic Helix-Loop-Helix Transcription Factors/*genetics ; China ; Genotype ; *Haplotypes ; Humans ; *Polymorphism, Single Nucleotide ; Tibet/ethnology ; }, abstract = {Tibetans have adapted to the extreme environment of high altitude for hundreds of generations. A highly differentiated 5-SNP (Single Nucleotide Polymorphism) haplotype motif (AGGAA) on a hypoxic pathway gene, EPAS1, is observed in Tibetans and lowlanders. To evaluate the potential usage of the 5-SNP haplotype in ancestry inference for Tibetan or Tibetan-related populations, we analyzed this haplotype in 1053 individuals of 12 Chinese populations residing on the Tibetan Plateau, peripheral regions of Tibet, and plain regions. These data were integrated with the genotypes from the 1000 Genome populations and populations in a previously reported paper for population structure analyses. We found that populations representing highland and lowland groups have different dominant ancestry components. The core Denisovan haplotype (AGGAA) was observed at a frequency of 72.32% in the Tibetan Plateau, with a frequency range from 9.48 to 21.05% in the peripheral regions and < 2.5% in the plains area. From the individual perspective, 87.57% of the individuals from the Tibetan Plateau carried the archaic haplotype, while < 5% of the Chinese Han people carried the haplotype. Our findings indicate that the 5-SNP haplotype has a special distribution pattern in populations of Tibet and peripheral regions and could be integrated into AISNP (Ancestry Informative Single Nucleotide Polymorphism) panels to enhance ancestry resolution.}, }
@article {pmid29422656, year = {2018}, author = {Rodríguez-Paredes, M and Bormann, F and Raddatz, G and Gutekunst, J and Lucena-Porcel, C and Köhler, F and Wurzer, E and Schmidt, K and Gallinat, S and Wenck, H and Röwert-Huber, J and Denisova, E and Feuerbach, L and Park, J and Brors, B and Herpel, E and Nindl, I and Hofmann, TG and Winnefeld, M and Lyko, F}, title = {Methylation profiling identifies two subclasses of squamous cell carcinoma related to distinct cells of origin.}, journal = {Nature communications}, volume = {9}, number = {1}, pages = {577}, pmid = {29422656}, issn = {2041-1723}, mesh = {Adult ; Aged ; Aged, 80 and over ; Carcinoma, Squamous Cell/*genetics ; Case-Control Studies ; Cell Differentiation ; DNA Methylation/*genetics ; Female ; *Gene Expression Regulation, Neoplastic ; Humans ; Keratinocytes ; Keratosis, Actinic/*genetics ; Male ; Middle Aged ; Skin Neoplasms/*genetics ; Young Adult ; }, abstract = {Cutaneous squamous cell carcinoma (cSCC) is the second most common skin cancer and usually progresses from a UV-induced precancerous lesion termed actinic keratosis (AK). Despite various efforts to characterize these lesions molecularly, the etiology of AK and its progression to cSCC remain partially understood. Here, we use Infinium MethylationEPIC BeadChips to interrogate the DNA methylation status in healthy, AK and cSCC epidermis samples. Importantly, we show that AK methylation patterns already display classical features of cancer methylomes and are highly similar to cSCC profiles. Further analysis identifies typical features of stem cell methylomes, such as reduced DNA methylation age, non-CpG methylation, and stem cell-related keratin and enhancer methylation patterns. Interestingly, this signature is detected only in half of the samples, while the other half shows patterns more closely related to healthy epidermis. These findings suggest the existence of two subclasses of AK and cSCC emerging from distinct keratinocyte differentiation stages.}, }
@article {pmid29383489, year = {2018}, author = {Yew, CW and Lu, D and Deng, L and Wong, LP and Ong, RT and Lu, Y and Wang, X and Yunus, Y and Aghakhanian, F and Mokhtar, SS and Hoque, MZ and Voo, CL and Abdul Rahman, T and Bhak, J and Phipps, ME and Xu, S and Teo, YY and Kumar, SV and Hoh, BP}, title = {Genomic structure of the native inhabitants of Peninsular Malaysia and North Borneo suggests complex human population history in Southeast Asia.}, journal = {Human genetics}, volume = {137}, number = {2}, pages = {161-173}, pmid = {29383489}, issn = {1432-1203}, support = {100-RM/BIOTEK 16/6/2 B (1/2011)//Kementerian Sains, Teknologi dan Inovasi (MY)/ ; [100-RMI/GOV 16/6/2 (19/2011]//Kementerian Sains, Teknologi dan Inovasi (MY)/ ; 100-RM/BIOTEK 16/6/2 B (1/2011)//Kementerian Sains, Teknologi dan Inovasi (MY)/ ; 100-RM/BIOTEK 16/6/2 B (1/2011)//Kementerian Sains, Teknologi dan Inovasi (MY)/ ; FRGS/1/2015/ST03/UCSI/01/1//Ministry of Higher Education, Malaysia (MY)/ ; NRF-RF-2010-05//National Foundation for Cancer Research (US)/ ; NRF-RF-2010-05//National Foundation for Cancer Research (US)/ ; NRF-RF-2010-05//National Research Foundation Singapore (SG)/ ; 91331204//National Natural Science Foundation of China (CN)/ ; 31525014//National Natural Science Foundation of China (CN)/ ; 91731303//National Natural Science Foundation of China (CN)/ ; 31771388//National Natural Science Foundation of China (CN)/ ; 31501011//National Natural Science Foundation of China (CN)/ ; 31711530221//National Institutes of Natural Sciences (JP)/ ; 10040231//Industry Strategic Development Program/ ; (1.150014.01)//Ulsan National Institute of Science and Technology (KR)/ ; }, mesh = {Asia, Southeastern ; Borneo ; DNA, Mitochondrial/*genetics ; Gene Flow/genetics ; Genetic Variation/*genetics ; *Genetics, Population ; Genome, Human/*genetics ; Genomics ; Human Migration ; Humans ; Malaysia ; Polymorphism, Single Nucleotide/genetics ; }, abstract = {Southeast Asia (SEA) is enriched with a complex history of peopling. Malaysia, which is located at the crossroads of SEA, has been recognized as one of the hubs for early human migration. To unravel the genomic complexity of the native inhabitants of Malaysia, we sequenced 12 samples from 3 indigenous populations from Peninsular Malaysia and 4 native populations from North Borneo to a high coverage of 28-37×. We showed that the Negritos from Peninsular Malaysia shared a common ancestor with the East Asians, but exhibited some level of gene flow from South Asia, while the North Borneo populations exhibited closer genetic affinity towards East Asians than the Malays. The analysis of time of divergence suggested that ancestors of Negrito were the earliest settlers in the Malay Peninsula, whom first separated from the Papuans ~ 50-33 thousand years ago (kya), followed by East Asian (~ 40-15 kya), while the divergence time frame between North Borneo and East Asia populations predates the Austronesian expansion period implies a possible pre-Neolithic colonization. Substantial Neanderthal ancestry was confirmed in our genomes, as was observed in other East Asians. However, no significant difference was observed, in terms of the proportion of Denisovan gene flow into these native inhabitants from Malaysia. Judging from the similar amount of introgression in the Southeast Asians and East Asians, our findings suggest that the Denisovan gene flow may have occurred before the divergence of these populations and that the shared similarities are likely an ancestral component.}, }
@article {pmid29320204, year = {2018}, author = {Shipilovskikh, SA and Vaganov, VY and Denisova, EI and Rubtsov, AE and Malkov, AV}, title = {Dehydration of Amides to Nitriles under Conditions of a Catalytic Appel Reaction.}, journal = {Organic letters}, volume = {20}, number = {3}, pages = {728-731}, doi = {10.1021/acs.orglett.7b03862}, pmid = {29320204}, issn = {1523-7052}, abstract = {A highly expedient protocol for a catalytic Appel-type dehydration of amides to nitriles has been developed that employs oxalyl chloride and triethylamine along with triphenylphosphine oxide as a catalyst. The reactions are usually complete in less than 10 min with only a 1 mol % catalyst loading. The reaction scope includes aromatic, heteroaromatic, and aliphatic amides, including derivatives of α-hydroxy and α-amino acids.}, }
@article {pmid29246791, year = {2018}, author = {Belousov, AB and Nishimune, H and Denisova, JV and Fontes, JD}, title = {A potential role for neuronal connexin 36 in the pathogenesis of amyotrophic lateral sclerosis.}, journal = {Neuroscience letters}, volume = {666}, number = {}, pages = {1-4}, pmid = {29246791}, issn = {1872-7972}, support = {P30 AG035982/AG/NIA NIH HHS/United States ; R01 NS078214/NS/NINDS NIH HHS/United States ; R01 AG051470/AG/NIA NIH HHS/United States ; UL1 TR000001/TR/NCATS NIH HHS/United States ; P30 HD002528/HD/NICHD NIH HHS/United States ; }, mesh = {Amyotrophic Lateral Sclerosis/*metabolism/*pathology ; Animals ; Connexins/*metabolism ; Disease Models, Animal ; Gap Junctions/metabolism ; Humans ; Mice ; Motor Neurons/metabolism ; Spinal Cord/metabolism ; Superoxide Dismutase-1/metabolism ; }, abstract = {Neuronal gap junctional protein connexin 36 (Cx36) contributes to neuronal death following a range of acute brain insults such as ischemia, traumatic brain injury and epilepsy. Whether Cx36 contributes to neuronal death and pathological outcomes in chronic neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS), is not known. We show here that the expression of Cx36 is significantly decreased in lumbar segments of the spinal cord of both human ALS subjects and SOD1G93A mice as compared to healthy human and wild-type mouse controls, respectively. In purified neuronal cultures prepared from the spinal cord of wild-type mice, knockdown of Cx36 reduces neuronal death caused by overexpression of the mutant human SOD1-G93A protein. Taken together, these data suggest a possible contribution of Cx36 to ALS pathogenesis. A perspective for the use of blockers of Cx36 gap junction channels for ALS therapy is discussed.}, }
@article {pmid29215085, year = {2018}, author = {Derenko, M and Denisova, G and Malyarchuk, B and Dambueva, I and Bazarov, B}, title = {Mitogenomic diversity and differentiation of the Buryats.}, journal = {Journal of human genetics}, volume = {63}, number = {1}, pages = {71-81}, pmid = {29215085}, issn = {1435-232X}, mesh = {Asian Continental Ancestry Group/*ethnology/*genetics ; DNA, Mitochondrial/*genetics ; Female ; *Genome, Mitochondrial ; Humans ; Male ; *Phylogeny ; Siberia/ethnology ; }, abstract = {In this paper we present a results of first comprehensive study of the complete mitogenomes in the Buryats with regard to their belonging to the main regional (eastern and western Buryats); tribal (Khori, Ekhirid, Bulagad, and Khongodor), and ethno-territorial (Aginsk, Alar, Balagansk, Barguzin, Ida, Khorinsk, Kuda, Selenga, Verkholensk, Olkhon, Tunka, and Shenehen Buryats) groups. The analysis of molecular variation performed using regional, tribal, and ethno-territorial divisions of the Buryats showed lack of genetic differentiation at all levels. Nonetheless, the complete mitogenome analysis revealed a very high level of genetic diversity in the Buryats which is the highest among Siberian populations and comparable to that in populations of eastern and western Asia. The AMOVA and MDS analyses results imply to a strong genetic similarity between the Buryats and eastern Asian populations of Chinese and Japanese, suggesting their origin on the basis of common maternal ancestry components. Several new Buryat-specific branches of haplogroup G (G2a2a, G2a1i, G2a5a) display signals of dispersals dating to 2.6-6.6 kya with a possible origin in eastern Asia, thus testifying Bronze Age and Neolithic arrival of ancestral eastern Asian component to the South Siberia region.}, }
@article {pmid29175608, year = {2018}, author = {Walter Costa, MB and Höner Zu Siederdissen, C and Tulpan, D and Stadler, PF and Nowick, K}, title = {Temporal ordering of substitutions in RNA evolution: Uncovering the structural evolution of the Human Accelerated Region 1.}, journal = {Journal of theoretical biology}, volume = {438}, number = {}, pages = {143-150}, doi = {10.1016/j.jtbi.2017.11.015}, pmid = {29175608}, issn = {1095-8541}, mesh = {*Evolution, Molecular ; Humans ; Models, Biological ; Mutation/genetics ; Nucleic Acid Conformation ; Probability ; RNA, Untranslated/*chemistry/*genetics ; Time Factors ; }, abstract = {The Human Accelerated Region 1 (HAR1) is the most rapidly evolving region in the human genome. It is part of two overlapping long non-coding RNAs, has a length of only 118 nucleotides and features 18 human specific changes compared to an ancestral sequence that is extremely well conserved across non-human primates. The human HAR1 forms a stable secondary structure that is strikingly different from the one in chimpanzee as well as other closely related species, again emphasizing its human-specific evolutionary history. This suggests that positive selection has acted to stabilize human-specific features in the ensemble of HAR1 secondary structures. To investigate the evolutionary history of the human HAR1 structure, we developed a computational model that evaluates the relative likelihood of evolutionary trajectories as a probabilistic version of a Hamiltonian path problem. The model predicts that the most likely last step in turning the ancestral primate HAR1 into the human HAR1 was exactly the substitution that distinguishes the modern human HAR1 sequence from that of Denisovan, an archaic human, providing independent support for our model. The MutationOrder software is available for download and can be applied to other instances of RNA structure evolution.}, }
@article {pmid29171471, year = {2017}, author = {Kovalkova, NA and Ragino, YI and Travnikova, NY and Denisova, DV and Shcherbakova, LV and Voevoda, MI}, title = {[Associations between metabolic syndrome and reduced lung function in young people].}, journal = {Terapevticheskii arkhiv}, volume = {89}, number = {10}, pages = {54-61}, doi = {10.17116/terarkh2017891054-61}, pmid = {29171471}, issn = {0040-3660}, mesh = {Adult ; Blood Glucose/analysis ; *Blood Pressure Determination/methods/statistics &amp; numerical data ; Body Mass Index ; Cardiovascular Diseases/*epidemiology ; Cholesterol/blood ; Cholesterol, HDL/blood ; Cholesterol, LDL/blood ; Female ; Humans ; Male ; *Metabolic Syndrome/blood/diagnosis/epidemiology/physiopathology ; Prevalence ; Respiratory Function Tests/methods/statistics &amp; numerical data ; *Respiratory Insufficiency/diagnosis/epidemiology/physiopathology ; Risk Factors ; Siberia/epidemiology ; Statistics as Topic ; Triglycerides/blood ; *Waist Circumference ; }, abstract = {AIM: To reveal possible associations between metabolic syndrome (MS) and reduced lung function.<br><br>SUBJECTS AND METHODS: In 2013-016, a cross-sectional survey was conducted in 908 Novosibirsk dwellers, which included spirometry to evaluate external respiratory function (ERF). For the detection of MS, the investigators used the 2009 All-Russian Research Society of Cardiologists criteria: waist circumference (WC) > 80 cm for women and >94 cm for men in combination with two of the following criteria: blood pressure (BP) ≥130/85 mm Hg, triglycerides (TG) ≥1.7 mmol/l, high-density lipoproteins (HDL) cholesterol <1.0 mmol/l for men and <1.2 mmol/l for women, low-density lipoprotein (LDL) cholesterol >3.0 mmol/l, and glucose ≥6.1 mmol/l.<br><br>RESULTS: The mean values of WC were significantly greater with a forced expiratory volume in one second (FEV1) <80% than those with a FEV1 of ≥80% in both men (p=0.002) and women (p=0.050); in women, the mean values of WS were higher than those with a FEV1/forced vital capacity (FVC) <70% than those with a FEV1/FVC of ≥70% (p=0.047); the mean systolic and diastolic BP levels were significantly more with reductions in FEV1 and FVC, and the level of HDL cholesterol was significantly lower than that with a FEV1 of < 80% in men only. Significant correlations were found between FEV1 and all components of MS in men, between the majority of components of MS and FVC in men, between WC, BP, and FEV1/FVC in men and women, between plasma glucose levels and FEV1/FVC in women. Linear regression analysis revealed significant inverse correlations of FEV1 with TG, glucose, BP; those of FVC with TG, glucose; at the same time a positive association with HDL cholesterol in men, and only a negative correlation of FEV1/FVC with WC.<br><br>CONCLUSION: The revealed associations between MS and reduced lung function can most likely be explained by the greater prevalence of both MS and its components (hypertension, hypertriglyceridemia, hyperglycemia, LDL hypercholesterolemia) among Novosibirsk men. This is consistent with the assertion that the decline in ERF, particularly FEV1, may be a marker of future cardiovascular disease morbidity and mortality.}, }
@article {pmid29072654, year = {2017}, author = {Soldatsky, YL and Denisova, OA and Bulynko, SA}, title = {[The specific features of the past medical history and etiology of pharyngeal abscess in the children].}, journal = {Vestnik otorinolaringologii}, volume = {82}, number = {5}, pages = {12-14}, doi = {10.17116/otorino201782512-14}, pmid = {29072654}, issn = {0042-4668}, mesh = {Adolescent ; Anti-Bacterial Agents/*therapeutic use ; Child ; Female ; Humans ; Male ; Medical History Taking ; Needs Assessment ; *Patient Care Management/methods/organization &amp; administration/standards ; *Peritonsillar Abscess/diagnosis/etiology/prevention &amp; control ; *Retropharyngeal Abscess/diagnosis/etiology/prevention &amp; control ; Risk Factors ; Russia/epidemiology ; *Streptococcal Infections/complications/diagnosis/drug therapy/epidemiology ; *Streptococcus pyogenes/isolation &amp; purification/pathogenicity ; Tonsillectomy/*methods ; *Tonsillitis/complications/diagnosis/epidemiology/therapy ; }, abstract = {The present study was undertaken for the purpose of elucidating the specific features of the past medical history and the etiological factors responsible for the development of tonsillogenic pharyngeal abscesses in the children. We performed the retrospective analysis of the medical histories of 291 children presenting with this condition who had been admitted for the treatment to the ENT Department of the Morozovskzya City Children's Clinical Hospital during the period from January till December 2015. The study has demonstrated the following most common shortcomings of the outpatient treatment of the patients suffering from chronic tonsillitis at the stage preceding formation of paratonsillar abscess: inadequate antibacterial therapy of acute chronic tonsillitis or its exacerbation and limited indications for tonsillectomy at the level of the outpatient treatment. The leading role in the etiology of tonsillogenic pharyngeal abscesses in the children is played by beta-hemolytic Streptococcus of group A. It is concluded that the medical history suggesting past paratonsillar abscess is the absolute indication for the subsequent tonsillectomy in the children of any age.}, }
@article {pmid29053564, year = {2018}, author = {Yazdanyar, A and Rizzuti, AE and Mechel, E and Denisova, K and Lazzaro, DR}, title = {Gout Keratitis: A Case of Peripheral Ulcerative Keratitis Secondary to Gout With a Review of the Literature.}, journal = {Cornea}, volume = {37}, number = {3}, pages = {379-381}, doi = {10.1097/ICO.0000000000001415}, pmid = {29053564}, issn = {1536-4798}, mesh = {Adult ; Corneal Stroma/pathology ; Corneal Ulcer/*etiology/metabolism/pathology ; Gout/*complications ; Humans ; Male ; Uric Acid/metabolism ; }, abstract = {PURPOSE: To report a case of peripheral ulcerative keratitis secondary to gout.<br><br>METHODS: A 41-year-old man with a history of severe gout disease presented with pain and redness of the right eye. Physical examination revealed 2 areas of peripheral corneal thinning with overlying epithelial defects. Adjacent to these areas, reflective crystals were identified in the corneal stroma. Anterior segment optical coherence tomography demonstrated stromal corneal deposits.<br><br>RESULTS: Systemic workup was negative aside from an elevated serum uric acid level. The patient was administered oral prednisone, allopurinol, and colchicine. At his 2-month follow-up visit, the patient was asymptomatic and his corneal thinning had significantly improved.<br><br>CONCLUSIONS: Gout is the most common type of inflammatory arthritis in adults with rising incidence and prevalence. Ocular findings in gout are common, but patients are usually asymptomatic. Monosodium urate crystal deposition has been reported to occur in various parts of the eye, with and without ocular inflammation. Crystal deposition in the cornea is extremely rare and may be a cause of peripheral ulcerative keratitis.}, }
@article {pmid28989973, year = {2017}, author = {Vashee, S and Stockwell, TB and Alperovich, N and Denisova, EA and Gibson, DG and Cady, KC and Miller, K and Kannan, K and Malouli, D and Crawford, LB and Voorhies, AA and Bruening, E and Caposio, P and Früh, K}, title = {Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination.}, journal = {mSphere}, volume = {2}, number = {5}, pages = {}, pmid = {28989973}, issn = {2379-5042}, support = {R41 AI106090/AI/NIAID NIH HHS/United States ; }, abstract = {Genetic engineering of cytomegalovirus (CMV) currently relies on generating a bacterial artificial chromosome (BAC) by introducing a bacterial origin of replication into the viral genome using in vivo recombination in virally infected tissue culture cells. However, this process is inefficient, results in adaptive mutations, and involves deletion of viral genes to avoid oversized genomes when inserting the BAC cassette. Moreover, BAC technology does not permit the simultaneous manipulation of multiple genome loci and cannot be used to construct synthetic genomes. To overcome these limitations, we adapted synthetic biology tools to clone CMV genomes in Saccharomyces cerevisiae. Using an early passage of the human CMV isolate Toledo, we first applied transformation-associated recombination (TAR) to clone 16 overlapping fragments covering the entire Toledo genome in Saccharomyces cerevisiae. Then, we assembled these fragments by TAR in a stepwise process until the entire genome was reconstituted in yeast. Since next-generation sequence analysis revealed that the low-passage-number isolate represented a mixture of parental and fibroblast-adapted genomes, we selectively modified individual DNA fragments of fibroblast-adapted Toledo (Toledo-F) and again used TAR assembly to recreate parental Toledo (Toledo-P). Linear, full-length HCMV genomes were transfected into human fibroblasts to recover virus. Unlike Toledo-F, Toledo-P displayed characteristics of primary isolates, including broad cellular tropism in vitro and the ability to establish latency and reactivation in humanized mice. Our novel strategy thus enables de novo cloning of CMV genomes, more-efficient genome-wide engineering, and the generation of viral genomes that are partially or completely derived from synthetic DNA. IMPORTANCE The genomes of large DNA viruses, such as human cytomegalovirus (HCMV), are difficult to manipulate using current genetic tools, and at this time, it is not possible to obtain, molecular clones of CMV without extensive tissue culture. To overcome these limitations, we used synthetic biology tools to capture genomic fragments from viral DNA and assemble full-length genomes in yeast. Using an early passage of the HCMV isolate Toledo containing a mixture of wild-type and tissue culture-adapted virus. we directly cloned the majority sequence and recreated the minority sequence by simultaneous modification of multiple genomic regions. Thus, our novel approach provides a paradigm to not only efficiently engineer HCMV and other large DNA viruses on a genome-wide scale but also facilitates the cloning and genetic manipulation of primary isolates and provides a pathway to generating entirely synthetic genomes.}, }
@article {pmid28980560, year = {2017}, author = {Saakyan, SV and Katargina, LA and Krichevskaya, GI and Myakoshina, EB and Denisova, EV}, title = {[Specific immunoglobulins G and M in blood serum in retinoblastoma and 'pseudoretinoblastoma'].}, journal = {Vestnik oftalmologii}, volume = {133}, number = {4}, pages = {12-16}, doi = {10.17116/oftalma2017133412-16}, pmid = {28980560}, issn = {0042-465X}, mesh = {Antibodies, Bacterial/*blood ; Antibodies, Viral/*blood ; Child, Preschool ; *Cytomegalovirus Retinitis/diagnosis/etiology ; Diagnosis, Differential ; Eye Abnormalities/*diagnosis ; Female ; Humans ; Immunoglobulin G/*blood ; Immunoglobulin M/*blood ; Infant ; Infant, Newborn ; Male ; Neonatal Screening ; Pregnancy ; Prenatal Exposure Delayed Effects/*diagnosis/microbiology ; Retina/abnormalities/microbiology ; *Retinal Neoplasms/diagnosis/immunology/microbiology/pathology ; *Retinoblastoma/diagnosis/immunology/microbiology/pathology ; }, abstract = {Perinatal inflammatory retinal diseases and intrauterine retinal maldevelopments are mistaken for retinoblastoma as often as in 8-16% of cases.<br><br>AIM: To analyze the infectious status in children with retinoblastoma and pseudoretinoblastoma at different ages.<br><br>MATERIAL AND METHODS: A total of 47 retinoblastoma suspects aged 4-69 months were enrolled. Pseudoretinoblastoma (inflammatory retinal diseases and intrauterine maldevelopments of the retina) was detected in 14 children (group 1), retinoblastoma - in 33 children (group 2). In each group, two subgroups were identified: 'a' - children under 12 months of age (1a - 5 patients, 2a - 10 patients) and 'b'- children over 12 months of age (1b - 9 patients, 2b - 23 patients). Their blood sera were examined for antibodies to herpes simplex virus types 1 and 2, cytomegalovirus, Epstein-Barr virus, toxoplasma, toxocara, chlamydia, and mycoplasma (enzyme-linked immunosorbent assay).<br><br>RESULTS: According to serological screening, all patients from group 1a (children under 12 months of age with pseudoretinoblastoma), in contrast to other groups, were infected perinatally with cytomegalovirus infection. All 47 patients were seronegative to toxoplasma. Toxocara infection was identified in children over 12 months of age: in 3 out of 9 patients with pseudoretinoblastoma and in 2 out of 23 patients with retinoblastoma (p>0.05). Markers of Epstein-Barr viral activity were detected only in 3 retinoblastoma children over 12 months of age.<br><br>CONCLUSION: The results suggest that cytomegalovirus infection plays the leading role in the development of perinatal eye pathology, which, in infants, is clinically similar to retinoblastoma. In children over 12 months of age we found no serological signs that could be regarded as specific of either retinoblastoma, or pseudoretinoblastoma. The only thing worth paying attention to is the activation of Epstein-Barr virus infection in children over 12 months of age with retinoblastoma.}, }
@article {pmid28950836, year = {2017}, author = {Xu, D and Jaber, Y and Pavlidis, P and Gokcumen, O}, title = {VCFtoTree: a user-friendly tool to construct locus-specific alignments and phylogenies from thousands of anthropologically relevant genome sequences.}, journal = {BMC bioinformatics}, volume = {18}, number = {1}, pages = {426}, pmid = {28950836}, issn = {1471-2105}, mesh = {Algorithms ; Animals ; Base Sequence ; *Genetic Loci ; *Genome, Human ; Humans ; INDEL Mutation/genetics ; *Phylogeny ; Primates ; Sequence Alignment/*methods ; Sequence Analysis, DNA ; *Software ; User-Computer Interface ; }, abstract = {BACKGROUND: Constructing alignments and phylogenies for a given locus from large genome sequencing studies with relevant outgroups allow novel evolutionary and anthropological insights. However, no user-friendly tool has been developed to integrate thousands of recently available and anthropologically relevant genome sequences to construct complete sequence alignments and phylogenies.<br><br>RESULTS: Here, we provide VCFtoTree, a user friendly tool with a graphical user interface that directly accesses online databases to download, parse and analyze genome variation data for regions of interest. Our pipeline combines popular sequence datasets and tree building algorithms with custom data parsing to generate accurate alignments and phylogenies using all the individuals from the 1000 Genomes Project, Neanderthal and Denisovan genomes, as well as reference genomes of Chimpanzee and Rhesus Macaque. It can also be applied to other phased human genomes, as well as genomes from other species. The output of our pipeline includes an alignment in FASTA format and a tree file in newick format.<br><br>CONCLUSION: VCFtoTree fulfills the increasing demand for constructing alignments and phylogenies for a given loci from thousands of available genomes. Our software provides a user friendly interface for a wider audience without prerequisite knowledge in programming. VCFtoTree can be accessed from https://github.com/duoduoo/VCFtoTree_3.0.0 .}, }
@article {pmid28902892, year = {2017}, author = {Zanolli, C and Hourset, M and Esclassan, R and Mollereau, C}, title = {Neanderthal and Denisova tooth protein variants in present-day humans.}, journal = {PloS one}, volume = {12}, number = {9}, pages = {e0183802}, pmid = {28902892}, issn = {1932-6203}, mesh = {Animals ; Dental Enamel/anatomy &amp; histology/metabolism ; Dental Enamel Proteins/*genetics/metabolism ; Fossils ; Gene Frequency ; Genome, Human ; Geography ; *Hominidae/genetics/metabolism ; Humans ; *Neanderthals/genetics/metabolism ; Organ Size ; Phylogeny ; *Polymorphism, Genetic ; Selection, Genetic ; Sequence Homology, Amino Acid ; Tooth/anatomy &amp; histology/chemistry/*metabolism ; }, abstract = {Environment parameters, diet and genetic factors interact to shape tooth morphostructure. In the human lineage, archaic and modern hominins show differences in dental traits, including enamel thickness, but variability also exists among living populations. Several polymorphisms, in particular in the non-collagenous extracellular matrix proteins of the tooth hard tissues, like enamelin, are involved in dental structure variation and defects and may be associated with dental disorders or susceptibility to caries. To gain insights into the relationships between tooth protein polymorphisms and dental structural morphology and defects, we searched for non-synonymous polymorphisms in tooth proteins from Neanderthal and Denisova hominins. The objective was to identify archaic-specific missense variants that may explain the dental morphostructural variability between extinct and modern humans, and to explore their putative impact on present-day dental phenotypes. Thirteen non-collagenous extracellular matrix proteins specific to hard dental tissues have been selected, searched in the publicly available sequence databases of Neanderthal and Denisova individuals and compared with modern human genome data. A total of 16 non-synonymous polymorphisms were identified in 6 proteins (ameloblastin, amelotin, cementum protein 1, dentin matrix acidic phosphoprotein 1, enamelin and matrix Gla protein). Most of them are encoded by dentin and enamel genes located on chromosome 4, previously reported to show signs of archaic introgression within Africa. Among the variants shared with modern humans, two are ancestral (common with apes) and one is the derived enamelin major variant, T648I (rs7671281), associated with a thinner enamel and specific to the Homo lineage. All the others are specific to Neanderthals and Denisova, and are found at a very low frequency in modern Africans or East and South Asians, suggesting that they may be related to particular dental traits or disease susceptibility in these populations. This modern regional distribution of archaic dental polymorphisms may reflect persistence of archaic variants in some populations and may contribute in part to the geographic dental variations described in modern humans.}, }
@article {pmid28900155, year = {2017}, author = {Denisova, K and Zhao, G}, title = {Inflexible neurobiological signatures precede atypical development in infants at high risk for autism.}, journal = {Scientific reports}, volume = {7}, number = {1}, pages = {11285}, pmid = {28900155}, issn = {2045-2322}, mesh = {Age Factors ; Autism Spectrum Disorder/diagnosis/*etiology/*psychology ; Brain Mapping ; Child ; Child, Preschool ; Disease Susceptibility ; Female ; Humans ; Infant ; Learning ; Magnetic Resonance Imaging ; Male ; Neuroimaging ; Neuropsychological Tests ; Phenotype ; Risk Assessment ; Risk Factors ; }, abstract = {Variability in neurobiological signatures is ubiquitous in early life but the link to adverse developmental milestones in humans is unknown. We examined how levels of signal and noise in movement signatures during the 1st year of life constrain early development in 71 healthy typically developing infants, either at High or Low familial Risk (HR or LR, respectively) for developing Autism Spectrum Disorders (ASD). Delays in early learning developmental trajectories in HR infants (validated in an analysis of 1,445 infants from representative infant-sibling studies) were predicted by worse stochastic patterns in their spontaneous head movements as early as 1-2 months after birth, relative to HR infants who showed more rapid developmental progress, as well as relative to all LR infants. While LR 1-2 mo-old infants' movements were significantly different during a language listening task compared to during sleep, HR infants' movements were more similar during both conditions, a striking lack of diversity that reveals context-inflexible experience of ambient information. Contrary to expectation, it is not the level of variability per se that is particularly detrimental in early life. Rather, inflexible sensorimotor systems and/or atypical transition between behavioral states may interfere with the establishment of capacity to extract structure and important cues from sensory input at birth, preceding and contributing to an atypical brain developmental trajectory in toddlerhood.}, }
@article {pmid28855259, year = {2017}, author = {Gardner, EJ and Lam, VK and Harris, DN and Chuang, NT and Scott, EC and Pittard, WS and Mills, RE and ,  and Devine, SE}, title = {The Mobile Element Locator Tool (MELT): population-scale mobile element discovery and biology.}, journal = {Genome research}, volume = {27}, number = {11}, pages = {1916-1929}, pmid = {28855259}, issn = {1549-5469}, support = {R01 HG002898/HG/NHGRI NIH HHS/United States ; R01 HG006849/HG/NHGRI NIH HHS/United States ; R01 GM059290/GM/NIGMS NIH HHS/United States ; R01 CA166661/CA/NCI NIH HHS/United States ; F31 HG009223/HG/NHGRI NIH HHS/United States ; T32 DK067872/DK/NIDDK NIH HHS/United States ; }, mesh = {Animals ; Computational Biology/*methods ; *DNA Transposable Elements ; Databases, Genetic ; Evolution, Molecular ; High-Throughput Nucleotide Sequencing/methods ; Humans ; Neanderthals/*genetics ; Pan troglodytes/*genetics ; Polymorphism, Single Nucleotide ; Software ; Whole Genome Sequencing/methods ; }, abstract = {Mobile element insertions (MEIs) represent ∼25% of all structural variants in human genomes. Moreover, when they disrupt genes, MEIs can influence human traits and diseases. Therefore, MEIs should be fully discovered along with other forms of genetic variation in whole genome sequencing (WGS) projects involving population genetics, human diseases, and clinical genomics. Here, we describe the Mobile Element Locator Tool (MELT), which was developed as part of the 1000 Genomes Project to perform MEI discovery on a population scale. Using both Illumina WGS data and simulations, we demonstrate that MELT outperforms existing MEI discovery tools in terms of speed, scalability, specificity, and sensitivity, while also detecting a broader spectrum of MEI-associated features. Several run modes were developed to perform MEI discovery on local and cloud systems. In addition to using MELT to discover MEIs in modern humans as part of the 1000 Genomes Project, we also used it to discover MEIs in chimpanzees and ancient (Neanderthal and Denisovan) hominids. We detected diverse patterns of MEI stratification across these populations that likely were caused by (1) diverse rates of MEI production from source elements, (2) diverse patterns of MEI inheritance, and (3) the introgression of ancient MEIs into modern human genomes. Overall, our study provides the most comprehensive map of MEIs to date spanning chimpanzees, ancient hominids, and modern humans and reveals new aspects of MEI biology in these lineages. We also demonstrate that MELT is a robust platform for MEI discovery and analysis in a variety of experimental settings.}, }
@article {pmid28854687, year = {2017}, author = {Jinam, TA and Phipps, ME and Aghakhanian, F and Majumder, PP and Datar, F and Stoneking, M and Sawai, H and Nishida, N and Tokunaga, K and Kawamura, S and Omoto, K and Saitou, N}, title = {Discerning the Origins of the Negritos, First Sundaland People: Deep Divergence and Archaic Admixture.}, journal = {Genome biology and evolution}, volume = {9}, number = {8}, pages = {2013-2022}, pmid = {28854687}, issn = {1759-6653}, mesh = {Asian Continental Ancestry Group/genetics ; *Genetics, Population ; *Genome, Human ; Genome-Wide Association Study ; Humans ; Malaysia ; Philippines ; *Phylogeny ; *Polymorphism, Single Nucleotide ; }, abstract = {Human presence in Southeast Asia dates back to at least 40,000 years ago, when the current islands formed a continental shelf called Sundaland. In the Philippine Islands, Peninsular Malaysia, and Andaman Islands, there exist indigenous groups collectively called Negritos whose ancestry can be traced to the "First Sundaland People." To understand the relationship between these Negrito groups and their demographic histories, we generated genome-wide single nucleotide polymorphism data in the Philippine Negritos and compared them with existing data from other populations. Phylogenetic tree analyses show that Negritos are basal to other East and Southeast Asians, and that they diverged from West Eurasians at least 38,000 years ago. We also found relatively high traces of Denisovan admixture in the Philippine Negritos, but not in the Malaysian and Andamanese groups, suggesting independent introgression and/or parallel losses involving Denisovan introgressed regions. Shared genetic loci between all three Negrito groups could be related to skin pigmentation, height, facial morphology and malarial resistance. These results show the unique status of Negrito groups as descended from the First Sundaland People.}, }
@article {pmid28854627, year = {2017}, author = {Sharbrough, J and Havird, JC and Noe, GR and Warren, JM and Sloan, DB}, title = {The Mitonuclear Dimension of Neanderthal and Denisovan Ancestry in Modern Human Genomes.}, journal = {Genome biology and evolution}, volume = {9}, number = {6}, pages = {1567-1581}, pmid = {28854627}, issn = {1759-6653}, mesh = {Alleles ; Animals ; Cell Nucleus/chemistry/*genetics ; DNA, Mitochondrial/chemistry/*genetics ; *Evolution, Molecular ; Gene Flow ; *Genome, Human ; Hominidae/classification/*genetics ; Humans ; Neanderthals/classification/*genetics ; Nucleic Acid Conformation ; Polymorphism, Genetic ; Reproductive Isolation ; }, abstract = {Some human populations interbred with Neanderthals and Denisovans, resulting in substantial contributions to modern-human genomes. Therefore, it is now possible to use genomic data to investigate mechanisms that shaped historical gene flow between humans and our closest hominin relatives. More generally, in eukaryotes, mitonuclear interactions have been argued to play a disproportionate role in generating reproductive isolation. There is no evidence of mtDNA introgression into modern human populations, which means that all introgressed nuclear alleles from archaic hominins must function on a modern-human mitochondrial background. Therefore, mitonuclear interactions are also potentially relevant to hominin evolution. We performed a detailed accounting of mtDNA divergence among hominin lineages and used population-genomic data to test the hypothesis that mitonuclear incompatibilities have preferentially restricted the introgression of nuclear genes with mitochondrial functions. We found a small but significant underrepresentation of introgressed Neanderthal alleles at such nuclear loci. Structural analyses of mitochondrial enzyme complexes revealed that these effects are unlikely to be mediated by physically interacting sites in mitochondrial and nuclear gene products. We did not detect any underrepresentation of introgressed Denisovan alleles at mitochondrial-targeted loci, but this may reflect reduced power because locus-specific estimates of Denisovan introgression are more conservative. Overall, we conclude that genes involved in mitochondrial function may have been subject to distinct selection pressures during the history of introgression from archaic hominins but that mitonuclear incompatibilities have had, at most, a small role in shaping genome-wide introgression patterns, perhaps because of limited functional divergence in mtDNA and interacting nuclear genes.}, }
@article {pmid28784789, year = {2017}, author = {Rogers, AR and Bohlender, RJ and Huff, CD}, title = {Early history of Neanderthals and Denisovans.}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {114}, number = {37}, pages = {9859-9863}, pmid = {28784789}, issn = {1091-6490}, support = {P30 CA016672/CA/NCI NIH HHS/United States ; R25 CA057730/CA/NCI NIH HHS/United States ; }, mesh = {Animals ; *Biological Evolution ; *Evolution, Molecular ; Fossils ; Gene Flow/*genetics ; Genome, Human/genetics ; Hominidae/*classification/*genetics ; Humans ; Neanderthals/classification/*genetics ; *Pedigree ; Phylogeny ; }, abstract = {Extensive DNA sequence data have made it possible to reconstruct human evolutionary history in unprecedented detail. We introduce a method to study the past several hundred thousand years. Our results show that (i) the Neanderthal-Denisovan lineage declined to a small size just after separating from the modern lineage, (ii) Neanderthals and Denisovans separated soon thereafter, and (iii) the subsequent Neanderthal population was large and deeply subdivided. They also (iv) support previous estimates of gene flow from Neanderthals into modern Eurasians. These results suggest an archaic human diaspora early in the Middle Pleistocene.}, }
@article {pmid28695206, year = {2017}, author = {Slon, V and Viola, B and Renaud, G and Gansauge, MT and Benazzi, S and Sawyer, S and Hublin, JJ and Shunkov, MV and Derevianko, AP and Kelso, J and Prüfer, K and Meyer, M and Pääbo, S}, title = {A fourth Denisovan individual.}, journal = {Science advances}, volume = {3}, number = {7}, pages = {e1700186}, pmid = {28695206}, issn = {2375-2548}, support = {//European Research Council/International ; }, abstract = {The presence of Neandertals in Europe and Western Eurasia before the arrival of anatomically modern humans is well supported by archaeological and paleontological data. In contrast, fossil evidence for Denisovans, a sister group of Neandertals recently identified on the basis of DNA sequences, is limited to three specimens, all of which originate from Denisova Cave in the Altai Mountains (Siberia, Russia). We report the retrieval of DNA from a deciduous lower second molar (Denisova 2), discovered in a deep stratigraphic layer in Denisova Cave, and show that this tooth comes from a female Denisovan individual. On the basis of the number of "missing substitutions" in the mitochondrial DNA determined from the specimen, we find that Denisova 2 is substantially older than two of the other Denisovans, reinforcing the view that Denisovans were likely to have been present in the vicinity of Denisova Cave over an extended time period. We show that the level of nuclear DNA sequence diversity found among Denisovans is within the lower range of that of present-day human populations.}, }
@article {pmid28669760, year = {2017}, author = {Margaryan, A and Derenko, M and Hovhannisyan, H and Malyarchuk, B and Heller, R and Khachatryan, Z and Avetisyan, P and Badalyan, R and Bobokhyan, A and Melikyan, V and Sargsyan, G and Piliposyan, A and Simonyan, H and Mkrtchyan, R and Denisova, G and Yepiskoposyan, L and Willerslev, E and Allentoft, ME}, title = {Eight Millennia of Matrilineal Genetic Continuity in the South Caucasus.}, journal = {Current biology : CB}, volume = {27}, number = {13}, pages = {2023-2028.e7}, doi = {10.1016/j.cub.2017.05.087}, pmid = {28669760}, issn = {1879-0445}, mesh = {Archaeology ; Armenia ; Azerbaijan ; Bayes Theorem ; DNA, Ancient/analysis ; *Gene Pool ; *Genetic Variation ; *Genome, Human ; *Genome, Mitochondrial ; Human Migration ; Humans ; }, abstract = {The South Caucasus, situated between the Black and Caspian Seas, geographically links Europe with the Near East and has served as a crossroad for human migrations for many millennia [1-7]. Despite a vast archaeological record showing distinct cultural turnovers, the demographic events that shaped the human populations of this region is not known [8, 9]. To shed light on the maternal genetic history of the region, we analyzed the complete mitochondrial genomes of 52 ancient skeletons from present-day Armenia and Artsakh spanning 7,800 years and combined this dataset with 206 mitochondrial genomes of modern Armenians. We also included previously published data of seven neighboring populations (n = 482). Coalescence-based analyses suggest that the population size in this region rapidly increased after the Last Glacial Maximum ca. 18 kya. We find that the lowest genetic distance in this dataset is between modern Armenians and the ancient individuals, as also reflected in both network analyses and discriminant analysis of principal components. We used approximate Bayesian computation to test five different demographic scenarios explaining the formation of the modern Armenian gene pool. Despite well documented cultural shifts in the South Caucasus across this time period, our results strongly favor a genetic continuity model in the maternal gene pool. This has implications for interpreting prehistoric migration dynamics and cultural shifts in this part of the world.}, }
@article {pmid28655834, year = {2017}, author = {Easton, JD and Aunes, M and Albers, GW and Amarenco, P and Bokelund-Singh, S and Denison, H and Evans, SR and Held, P and Jahreskog, M and Jonasson, J and Minematsu, K and Molina, CA and Wang, Y and Wong, KSL and Johnston, SC and , }, title = {Risk for Major Bleeding in Patients Receiving Ticagrelor Compared With Aspirin After Transient Ischemic Attack or Acute Ischemic Stroke in the SOCRATES Study (Acute Stroke or Transient Ischemic Attack Treated With Aspirin or Ticagrelor and Patient Outcomes).}, journal = {Circulation}, volume = {136}, number = {10}, pages = {907-916}, doi = {10.1161/CIRCULATIONAHA.117.028566}, pmid = {28655834}, issn = {1524-4539}, mesh = {Adenosine/administration &amp; dosage/adverse effects/*analogs &amp; derivatives ; Aged ; Aspirin/administration &amp; dosage/*adverse effects ; Female ; Hemorrhage/*chemically induced ; Humans ; Ischemic Attack, Transient/*complications/drug therapy ; Male ; Purinergic P2Y Receptor Antagonists/administration &amp; dosage/*adverse effects ; Risk ; Stroke/*complications/drug therapy ; Ticagrelor ; Treatment Outcome ; }, abstract = {BACKGROUND: Patients with minor acute ischemic stroke or transient ischemic attack are at high risk for subsequent stroke, and more potent antiplatelet therapy in the acute setting is needed. However, the potential benefit of more intense antiplatelet therapy must be assessed in relation to the risk for major bleeding. The SOCRATES trial (Acute Stroke or Transient Ischemic Attack Treated With Aspirin or Ticagrelor and Patient Outcomes) was the first trial with ticagrelor in patients with acute ischemic stroke or transient ischemic attack in which the efficacy and safety of ticagrelor were compared with those of aspirin. The main safety objective was assessment of PLATO (Platelet Inhibition and Patient Outcomes)-defined major bleeds on treatment, with special focus on intracranial hemorrhage (ICrH).<br><br>METHODS: An independent adjudication committee blinded to study treatment classified bleeds according to the PLATO, TIMI (Thrombolysis in Myocardial Infarction), and GUSTO (Global Use of Strategies to Open Occluded Coronary Arteries) definitions. The definitions of ICrH and major bleeding excluded cerebral microbleeds and asymptomatic hemorrhagic transformations of cerebral infarctions so that the definitions better discriminated important events in the acute stroke population.<br><br>RESULTS: A total of 13 130 of 13 199 randomized patients received at least 1 dose of study drug and were included in the safety analysis set. PLATO major bleeds occurred in 31 patients (0.5%) on ticagrelor and 38 patients (0.6%) on aspirin (hazard ratio, 0.83; 95% confidence interval, 0.52-1.34). The most common locations of major bleeds were intracranial and gastrointestinal. ICrH was reported in 12 patients (0.2%) on ticagrelor and 18 patients (0.3%) on aspirin. Thirteen of all 30 ICrHs (4 on ticagrelor and 9 on aspirin) were hemorrhagic strokes, and 4 (2 in each group) were symptomatic hemorrhagic transformations of brain infarctions. The ICrHs were spontaneous in 6 and 13, traumatic in 3 and 3, and procedural in 3 and 2 patients on ticagrelor and aspirin, respectively. In total, 9 fatal bleeds occurred on ticagrelor and 4 on aspirin. The composite of ICrH or fatal bleeding included 15 patients on ticagrelor and 18 on aspirin. Independently of bleeding classification, PLATO, TIMI, or GUSTO, the relative difference between treatments for major/severe bleeds was similar. Nonmajor bleeds were more common on ticagrelor.<br><br>CONCLUSIONS: Antiplatelet therapy with ticagrelor in patients with acute ischemic stroke or transient ischemic attack showed a bleeding profile similar to that of aspirin for major bleeds. There were few ICrHs.<br><br>CLINICAL TRIAL REGISTRATION: URL: http://www.clinicaltrials.gov. Unique identifier: NCT01994720.}, }
@article {pmid28615036, year = {2017}, author = {Alexeeva, EI and Namazova-Baranova, LS and Bzarova, TM and Valieva, SI and Denisova, RV and Sleptsova, TV and Isaeva, KB and Chomahidze, AM and Taibulatov, NI and Fetisova, AN and Karaseva, AV and Baranov, AA}, title = {Predictors of the response to etanercept in patients with juvenile idiopathic arthritis without systemic manifestations within 12 months: results of an open-label, prospective study conducted at the National Scientific and Practical Center of Children's Health, Russia.}, journal = {Pediatric rheumatology online journal}, volume = {15}, number = {1}, pages = {51}, pmid = {28615036}, issn = {1546-0096}, mesh = {Age of Onset ; Antirheumatic Agents/administration &amp; dosage/adverse effects ; *Arthritis, Juvenile/diagnosis/drug therapy/epidemiology ; C-Reactive Protein/analysis ; Child ; Child, Preschool ; Drug Monitoring/methods ; *Etanercept/administration &amp; dosage/adverse effects ; Female ; Humans ; Male ; Outcome and Process Assessment, Health Care ; Patient Acuity ; Range of Motion, Articular/drug effects ; Russia/epidemiology ; Severity of Illness Index ; }, abstract = {BACKGROUND: The aim of this study was to investigate the efficacy of etanercept treatment and to identify predictors of response to therapy within 12 months in patients with juvenile idiopathic arthritis (JIA) without systemic manifestations.<br><br>METHODS: A total of 197 juvenile patients were enrolled in this study. Response to therapy was assessed using the ACRPedi 30/50/70/90 criteria, the Wallace criteria, and the Juvenile Arthritis Disease Activity Score 71 (JADAS-71). Univariate and multivariate logistic regression analyses were performed to identify potential baseline factors associated with treatment response in different JIA categories.<br><br>RESULTS: One year after treatment initiation, 179 (90.9%) patients achieved ACRPedi30; 177 (89.8%) patients achieved ACRPedi50; 168 (85.3%) patients achieved ACRPedi70; and 135 (68.5%) patients achieved ACRPedi90 response. A total of 132 (67.0%) and 92 (46.7%) patients achieved inactive disease according to the Wallace criteria and the JADAS-71 cut-off point, respectively. Excellent response (achieving ACRPedi90 and clinically inactive disease according both to the Wallace criteria and the JADAS71 cut-off point) was associated with persistent oligoarticular JIA category, shorter disease duration before the start of etanercept, a lower number of DMARDs used before the introduction of etanercept, a lower number of joints with limited motion, and lower C-reactive protein at baseline. Poor response (failure to achieve ACR 70 or active disease according to both the Wallace criteria and JADAS71 even when ACR 70 was achieved) was associated with the polyarticular or enthesitis-related JIA categories, higher disease duration before the start of etanercept, and older age at disease onset.<br><br>CONCLUSION: Almost half (45.7%) of the patients who initiated etanercept treatment achieved an excellent response (inactive disease and ACRPedi90) after 1 year. What may be novel is our finding that the response to etanercept therapy was strongly associated with the JIA category. The response to etanercept therapy was also associated with the disease duration before the start of etanercept treatment.}, }
@article {pmid28572766, year = {2017}, author = {Guo, S and Lai, C and Wu, C and Cen, G and , }, title = {Conversion Discriminative Analysis on Mild Cognitive Impairment Using Multiple Cortical Features from MR Images.}, journal = {Frontiers in aging neuroscience}, volume = {9}, number = {}, pages = {146}, pmid = {28572766}, issn = {1663-4365}, support = {K23 NS083711/NS/NINDS NIH HHS/United States ; P30 AG010129/AG/NIA NIH HHS/United States ; P50 AG005142/AG/NIA NIH HHS/United States ; }, abstract = {Neuroimaging measurements derived from magnetic resonance imaging provide important information required for detecting changes related to the progression of mild cognitive impairment (MCI). Cortical features and changes play a crucial role in revealing unique anatomical patterns of brain regions, and further differentiate MCI patients from normal states. Four cortical features, namely, gray matter volume, cortical thickness, surface area, and mean curvature, were explored for discriminative analysis among three groups including the stable MCI (sMCI), the converted MCI (cMCI), and the normal control (NC) groups. In this study, 158 subjects (72 NC, 46 sMCI, and 40 cMCI) were selected from the Alzheimer's Disease Neuroimaging Initiative. A sparse-constrained regression model based on the l2-1-norm was introduced to reduce the feature dimensionality and retrieve essential features for the discrimination of the three groups by using a support vector machine (SVM). An optimized strategy of feature addition based on the weight of each feature was adopted for the SVM classifier in order to achieve the best classification performance. The baseline cortical features combined with the longitudinal measurements for 2 years of follow-up data yielded prominent classification results. In particular, the cortical thickness produced a classification with 98.84% accuracy, 97.5% sensitivity, and 100% specificity for the sMCI-cMCI comparison; 92.37% accuracy, 84.78% sensitivity, and 97.22% specificity for the cMCI-NC comparison; and 93.75% accuracy, 92.5% sensitivity, and 94.44% specificity for the sMCI-NC comparison. The best performances obtained by the SVM classifier using the essential features were 5-40% more than those using all of the retained features. The feasibility of the cortical features for the recognition of anatomical patterns was certified; thus, the proposed method has the potential to improve the clinical diagnosis of sub-types of MCI and predict the risk of its conversion to Alzheimer's disease.}, }
@article {pmid28514400, year = {2017}, author = {Denisova, OA and Livzan, MA and Denisov, AP}, title = {[Comparative characteristics of patients with gastroesophageal reflux disease in the age aspect].}, journal = {Terapevticheskii arkhiv}, volume = {89}, number = {4}, pages = {53-56}, doi = {10.17116/terarkh201789453-56}, pmid = {28514400}, issn = {0040-3660}, mesh = {Adolescent ; Adult ; Aged ; Aged, 80 and over ; Esophageal pH Monitoring ; *Esophagitis, Peptic/etiology ; *Gastroesophageal Reflux/complications/diagnosis ; Humans ; Middle Aged ; Young Adult ; }, abstract = {AIM: To compare the characteristics of patients with gastroesophageal reflux disease (GERD) by age groups, a wide range of clinical signs, including life-of-quality (QOL) indicators, and instrumental findings.<br><br>SUBJECTS AND METHODS: A total of 110 patients aged 18 to 86 years with GERD were examined in accordance with the standard protocol. Two groups with equal numbers of patents were formed. A study group included elderly and senile patients and a control group consisted of young and adult ones.<br><br>RESULTS: The elderly patients with GERD were observed to have a number of age-related clinical features and age-unrelated symptoms. The scores in the scales characterizing the physical health component and those in the general health and vital activity scales were markedly decreased in patients older than 60 years of age. No age-related statistically significant differences were found in the esophageal, gastric, and duodenal mucosae. Daily pH-metry in the elderly showed indirect evidence for esophageal hypomotor dyskinesia in the predominance of alkaline refluxes.<br><br>CONCLUSION: The cohort of elderly patients with GERD was ascertained to have statistically significant clinical characteristics, and QOL and pH-metry indicators, which will be able to improve methods for diagnosis and early prevention in this age group.}, }
@article {pmid28464262, year = {2017}, author = {Reales, G and Rovaris, DL and Jacovas, VC and Hünemeier, T and Sandoval, JR and Salazar-Granara, A and Demarchi, DA and Tarazona-Santos, E and Felkl, AB and Serafini, MA and Salzano, FM and Bisso-Machado, R and Comas, D and Paixão-Côrtes, VR and Bortolini, MC}, title = {A tale of agriculturalists and hunter-gatherers: Exploring the thrifty genotype hypothesis in native South Americans.}, journal = {American journal of physical anthropology}, volume = {163}, number = {3}, pages = {591-601}, doi = {10.1002/ajpa.23233}, pmid = {28464262}, issn = {1096-8644}, mesh = {Agriculture/*history ; Anthropology, Physical ; Apolipoproteins E/genetics ; CD36 Antigens/genetics ; Genotype ; History, Ancient ; Humans ; Indians, South American/*genetics/*history ; Polymorphism, Single Nucleotide/*genetics ; RNA-Binding Proteins/genetics ; }, abstract = {OBJECTIVES: To determine genetic differences between agriculturalist and hunter-gatherer southern Native American populations for selected metabolism-related markers and to test whether Neel's thrifty genotype hypothesis (TGH) could explain the genetic patterns observed in these populations.<br><br>MATERIALS AND METHODS: 375 Native South American individuals from 17 populations were genotyped using six markers (APOE rs429358 and rs7412; APOA2 rs5082; CD36 rs3211883; TCF7L2 rs11196205; and IGF2BP2 rs11705701). Additionally, APOE genotypes from 39 individuals were obtained from the literature. AMOVA, main effects, and gene-gene interaction tests were performed.<br><br>RESULTS: We observed differences in allele distribution patterns between agriculturalists and hunter-gatherers for some markers. For instance, between-groups component of genetic variance (FCT) for APOE rs429358 showed strong differences in allelic distributions between hunter-gatherers and agriculturalists (p = 0.00196). Gene-gene interaction analysis indicated that the APOE E4/CD36 TT and APOE E4/IGF2BP2 A carrier combinations occur at a higher frequency in hunter-gatherers, but this combination is not replicated in archaic (Neanderthal and Denisovan) and ancient (Anzick, Saqqaq, Ust-Ishim, Mal'ta) hunter-gatherer individuals.<br><br>DISCUSSION: A complex scenario explains the observed frequencies of the tested markers in hunter-gatherers. Different factors, such as pleotropic alleles, rainforest selective pressures, and population dynamics, may be collectively shaping the observed genetic patterns. We conclude that although TGH seems a plausible hypothesis to explain part of the data, other factors may be important in our tested populations.}, }
@article {pmid28450384, year = {2017}, author = {Slon, V and Hopfe, C and Weiß, CL and Mafessoni, F and de la Rasilla, M and Lalueza-Fox, C and Rosas, A and Soressi, M and Knul, MV and Miller, R and Stewart, JR and Derevianko, AP and Jacobs, Z and Li, B and Roberts, RG and Shunkov, MV and de Lumley, H and Perrenoud, C and Gušić, I and Kućan, Ž and Rudan, P and Aximu-Petri, A and Essel, E and Nagel, S and Nickel, B and Schmidt, A and Prüfer, K and Kelso, J and Burbano, HA and Pääbo, S and Meyer, M}, title = {Neandertal and Denisovan DNA from Pleistocene sediments.}, journal = {Science (New York, N.Y.)}, volume = {356}, number = {6338}, pages = {605-608}, doi = {10.1126/science.aam9695}, pmid = {28450384}, issn = {1095-9203}, mesh = {Animals ; Caves ; DNA, Ancient/analysis/*isolation &amp; purification ; DNA, Mitochondrial/analysis/*isolation &amp; purification ; Europe ; Fossils ; Geologic Sediments/chemistry ; Hominidae/*classification/*genetics ; Sequence Analysis, DNA ; }, abstract = {Although a rich record of Pleistocene human-associated archaeological assemblages exists, the scarcity of hominin fossils often impedes the understanding of which hominins occupied a site. Using targeted enrichment of mitochondrial DNA, we show that cave sediments represent a rich source of ancient mammalian DNA that often includes traces of hominin DNA, even at sites and in layers where no hominin remains have been discovered. By automation-assisted screening of numerous sediment samples, we detected Neandertal DNA in eight archaeological layers from four caves in Eurasia. In Denisova Cave, we retrieved Denisovan DNA in a Middle Pleistocene layer near the bottom of the stratigraphy. Our work opens the possibility of detecting the presence of hominin groups at sites and in areas where no skeletal remains are found.}, }
@article {pmid28448578, year = {2017}, author = {Hu, H and Petousi, N and Glusman, G and Yu, Y and Bohlender, R and Tashi, T and Downie, JM and Roach, JC and Cole, AM and Lorenzo, FR and Rogers, AR and Brunkow, ME and Cavalleri, G and Hood, L and Alpatty, SM and Prchal, JT and Jorde, LB and Robbins, PA and Simonson, TS and Huff, CD}, title = {Evolutionary history of Tibetans inferred from whole-genome sequencing.}, journal = {PLoS genetics}, volume = {13}, number = {4}, pages = {e1006675}, pmid = {28448578}, issn = {1553-7404}, support = {R00 HL118215/HL/NHLBI NIH HHS/United States ; R35 GM118335/GM/NIGMS NIH HHS/United States ; 089457/Z/09/Z//Wellcome Trust/United Kingdom ; P50 GM076547/GM/NIGMS NIH HHS/United States ; }, mesh = {Adaptation, Physiological/*genetics ; Altitude ; Basic Helix-Loop-Helix Transcription Factors/*genetics ; Cytochrome P-450 Enzyme System/genetics ; Female ; *Genome, Human ; Haplotypes ; High-Throughput Nucleotide Sequencing ; Humans ; Hypoxia-Inducible Factor-Proline Dioxygenases/genetics ; Male ; Molecular Sequence Annotation ; Proteins/genetics ; Receptors, Calcitriol/genetics ; Selection, Genetic/*genetics ; Tibet ; }, abstract = {The indigenous people of the Tibetan Plateau have been the subject of much recent interest because of their unique genetic adaptations to high altitude. Recent studies have demonstrated that the Tibetan EPAS1 haplotype is involved in high altitude-adaptation and originated in an archaic Denisovan-related population. We sequenced the whole-genomes of 27 Tibetans and conducted analyses to infer a detailed history of demography and natural selection of this population. We detected evidence of population structure between the ancestral Han and Tibetan subpopulations as early as 44 to 58 thousand years ago, but with high rates of gene flow until approximately 9 thousand years ago. The CMS test ranked EPAS1 and EGLN1 as the top two positive selection candidates, and in addition identified PTGIS, VDR, and KCTD12 as new candidate genes. The advantageous Tibetan EPAS1 haplotype shared many variants with the Denisovan genome, with an ancient gene tree divergence between the Tibetan and Denisovan haplotypes of about 1 million years ago. With the exception of EPAS1, we observed no evidence of positive selection on Denisovan-like haplotypes.}, }
@article {pmid28444387, year = {2017}, author = {Jégou, B and Sankararaman, S and Rolland, AD and Reich, D and Chalmel, F}, title = {Meiotic Genes Are Enriched in Regions of Reduced Archaic Ancestry.}, journal = {Molecular biology and evolution}, volume = {34}, number = {8}, pages = {1974-1980}, pmid = {28444387}, issn = {1537-1719}, support = {R00 GM111744/GM/NIGMS NIH HHS/United States ; R01 GM100233/GM/NIGMS NIH HHS/United States ; /HHMI/Howard Hughes Medical Institute/United States ; }, mesh = {Alleles ; Animals ; Databases, Genetic ; Evolution, Molecular ; Gene Expression Regulation, Developmental/genetics ; Genome, Human/genetics ; Genomics ; Hominidae/*genetics ; Humans ; Male ; Meiosis/*genetics ; Neanderthals/*genetics ; Selection, Genetic ; Testis ; }, abstract = {About 1-6% of the genetic ancestry of modern humans today originates from admixture with archaic humans. It has recently been shown that autosomal genomic regions with a reduced proportion of Neanderthal and Denisovan ancestries (NA and DA) are significantly enriched in genes that are more expressed in testis than in other tissues. To determine whether a cellular segregation pattern would exist, we combined maps of archaic introgression with a cross-analysis of three transcriptomic datasets deciphering the transcriptional landscape of human gonadal cell types. We reveal that the regions deficient in both NA and DA contain a significant enrichment of genes transcribed in meiotic germ cells. The interbreeding of anatomically modern humans with archaic humans may have introduced archaic-derived alleles that contributed to genetic incompatibilities affecting meiosis that were subsequently purged by natural selection.}, }
@article {pmid28434540, year = {2017}, author = {Ao, H and Liu, CR and Roberts, AP and Zhang, P and Xu, X}, title = {An updated age for the Xujiayao hominin from the Nihewan Basin, North China: Implications for Middle Pleistocene human evolution in East Asia.}, journal = {Journal of human evolution}, volume = {106}, number = {}, pages = {54-65}, doi = {10.1016/j.jhevol.2017.01.014}, pmid = {28434540}, issn = {1095-8606}, mesh = {Animals ; China ; *Electron Spin Resonance Spectroscopy ; *Fossils ; *Hominidae ; Humans ; Molar ; Neanderthals ; Time Factors ; }, abstract = {The Xujiayao site in the Nihewan Basin (North China) is one of the most important Paleolithic sites in East Asia. Twenty Homo fossils, which were previously assigned to an archaic Homo sapiens group, have been excavated along with more than 30,000 lithic artifacts and ∼5000 mammalian fossil specimens. Dating of the Xujiayao hominin has been pursued since its excavation in the 1970s, but its age has remained controversial because of limitations of the dating techniques that have been applied to available materials. Here, we report new ages for the Xujiayao hominin based on combined electron spin resonance (ESR) dating of quartz in the sediments and high-resolution magnetostratigraphy of the fluvio-lacustrine sequence. The magnetostratigraphy suggests that the upper Matuyama and Brunhes polarity chrons are recorded at Xujiayao. The ESR dating results indicate a pooled average age of 260-370 ka for the Homo-bearing layer, which is consistent with its position within the middle Brunhes normal polarity chron indicated by magnetostratigraphy. This age estimate makes the Xujiayao hominin among the oldest mid-Pleistocene hominins with derived Neanderthal traits in East Asia. This age is consistent with the time when early Denisovans, a sister group of Neanderthals, appeared and colonized eastern Eurasia. Our updated age and the Neanderthal-like traits of the Xujiayao Homo fossils, particularly the Denisovan-like molar teeth, make it possible that the Xujiayao hominin could represent an early Denisovan.}, }
@article {pmid28410231, year = {2017}, author = {Heidenreich, B and Denisova, E and Rachakonda, S and Sanmartin, O and Dereani, T and Hosen, I and Nagore, E and Kumar, R}, title = {Genetic alterations in seborrheic keratoses.}, journal = {Oncotarget}, volume = {8}, number = {22}, pages = {36639-36649}, pmid = {28410231}, issn = {1949-2553}, mesh = {Adult ; Aged ; Aged, 80 and over ; Alleles ; Biomarkers ; DNA Mutational Analysis ; Female ; Gene Expression ; *Genetic Association Studies ; *Genetic Predisposition to Disease ; *Genetic Variation ; Genotype ; Humans ; Keratosis, Seborrheic/*genetics ; Male ; Middle Aged ; Mutation ; Polymorphism, Single Nucleotide ; Whole Exome Sequencing ; }, abstract = {Seborrheic keratoses are common benign epidermal lesions that are associated with increased age and sun-exposure. Those lesions despite harboring multiple somatic alterations in contrast to malignant tumors appear to be genetically stable. In order to investigate and characterize the presence of recurrent mutations, we performed exome sequencing on DNA from one seborrheic keratosis lesion and corresponding blood cells from the same patients with follow up investigation of alterations identified by exome sequencing in 24 additional lesions from as many patients. In addition we investigated alterations in all lesions at specific genes loci that included FGFR3, PIK3CA, HRAS, BRAF, CDKN2A and TERT and DHPH3 promoters. The exome sequencing data indicated three mutations per Mb of the targeted sequence. The mutational pattern depicted typical UV signature with majority of alterations being C>T and CC>TT base changes at dipyrimidinic sites. The FGFR3 mutations were the most frequent, detected in 12 of 25 (48%) lesions, followed by the PIK3CA (32%), TERT promoter (24%) and DPH3 promoter mutations (24%). TERT promoter mutations associated with increased age and were present mainly in the lesions excised from head and neck. Three lesions also carried alterations in CDKN2A. FGFR3, TERT and DPH3 expression did not correlate with mutations in the respective genes and promoters; however, increased FGFR3 transcript levels were associated with increased FOXN1 levels, a suggested positive feedback loop that stalls malignant progression. Thus, in this study we report overall mutation rate through exome sequencing and show the most frequent mutations seborrheic keratosis.}, }
@article {pmid28408228, year = {2017}, author = {Kharitonova, MI and Denisova, AO and Andronova, VL and Kayushin, AL and Konstantinova, ID and Kotovskaya, SK and Galegov, GA and Charushin, VN and Miroshnikov, AI}, title = {New modified 2-aminobenzimidazole nucleosides: Synthesis and evaluation of their activity against herpes simplex virus type 1.}, journal = {Bioorganic &amp; medicinal chemistry letters}, volume = {27}, number = {11}, pages = {2484-2487}, doi = {10.1016/j.bmcl.2017.03.100}, pmid = {28408228}, issn = {1464-3405}, mesh = {Acyclovir/pharmacology ; Antiviral Agents/*chemical synthesis/pharmacology ; Benzimidazoles/*chemistry ; Cidofovir ; Cytosine/analogs &amp; derivatives/pharmacology ; Drug Resistance, Viral/drug effects ; Foscarnet/pharmacology ; Herpesvirus 1, Human/drug effects ; Humans ; Nucleosides/*chemistry/pharmacology ; Organophosphonates/pharmacology ; }, abstract = {Using the enzymatic transglycosylation reaction β-d-ribo- and 2'-deoxyribofuranosides of 2-amino-5,6-difluorobenzimidazole nucleosides have been synthesized. 2-Amino-5,6-difluoro-benzimidazole riboside proved to exhibit a selective antiviral activity (selectivity index >32) against a wild strain of the herpes simplex virus type 1, as well as towards virus strains that are resistant to acyclovir, cidofovir, and foscarnet. We believe that this compound might be used for treatment of herpes infections in those cases, when acyclovir is not efficient.}, }
@article {pmid28346564, year = {2017}, author = {Hu, Y and Malyutina, S and Pikhart, H and Peasey, A and Holmes, MV and Hubacek, J and Denisova, D and Nikitin, Y and Bobak, M}, title = {The Relationship between Body Mass Index and 10-Year Trajectories of Physical Functioning in Middle-Aged and Older Russians: Prospective Results of the Russian HAPIEE Study.}, journal = {The journal of nutrition, health &amp; aging}, volume = {21}, number = {4}, pages = {381-388}, pmid = {28346564}, issn = {1760-4788}, support = {081081/Z/06/Z//Wellcome Trust/United Kingdom ; }, mesh = {Aged ; Aging/*physiology ; Body Height ; Body Mass Index ; Body Weight ; Cohort Studies ; Data Collection ; Female ; *Health Status ; Humans ; Male ; Middle Aged ; Obesity/epidemiology/*pathology ; Physical Fitness/*physiology ; Prospective Studies ; Risk Factors ; Russia/epidemiology ; }, abstract = {OBJECTIVE: To investigate the associations of overweight and obesity with longitudinal decline in physical functioning (PF) among middle-aged and older Russians.<br><br>DESIGN: Prospective cohort study.<br><br>SETTING: Four rounds of data collection in the Russian Health, Alcohol and Psychosocial factors In Eastern Europe study with up to 10 years of follow-up.<br><br>PARTICIPANTS: 9,222 men and women aged 45-69 years randomly selected from the population of two districts of Novosibirsk, Russia.<br><br>MEASUREMENTS: PF score (range 0-100) was measured by the Physical Functioning Subscale (PF-10) of the 36-item Short Form Health Survey (SF-36) at baseline and three subsequent occasions. Body mass index (BMI), derived from objectively measured body height and weight at baseline, was classified into normal weight (BMI 18.5-24.9), overweight (BMI 25.0-29.9), obesity class I (BMI 30.0-34.9), and obesity class II+ (BMI≥35.0).<br><br>RESULTS: The mean annual decline in the PF score during the follow-up was -1.92 (95% confidence interval -2.17; -1.68) in men and -1.91 (-2.13; -1.68) in women. At baseline, compared with normal weight, obesity classes I and II+ (but not overweight) were associated with significantly lower PF in both sexes. In prospective analyses, the decline in PF was faster in overweight men (difference from normal weight subjects -0.38 [-0.63; -0.14]), class I obese men and women (-0.49 [-0.82; -0.17] and -0.44 [-0.73; -0.15] respectively) and class II+ obese men and women (-1.13 [-1.73; -0.53] and -0.43 [-0.77; -0.09] respectively). Adjustment for physical activity and other covariates did not materially change the results.<br><br>CONCLUSIONS: PF decreased more rapidly in obese men and women than among those with normal weight. The adverse effect of high BMI on PF trajectories appeared to be more pronounced in men than in women, making more extremely obese Russian men an important target population to prevent/slow down the process of decline in PF.}, }
@article {pmid28282561, year = {2017}, author = {Baklanova, YV and Lipina, OA and Maksimova, LG and Tyutyunnik, AP and Leonidov, II and Denisova, TA and Zubkov, VG}, title = {Nd3+, Ho3+-codoped garnet-related Li7La3Hf2O12 phosphor with NIR luminescence.}, journal = {Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy}, volume = {180}, number = {}, pages = {105-109}, doi = {10.1016/j.saa.2017.03.007}, pmid = {28282561}, issn = {1873-3557}, abstract = {Simultaneous emission lines around 1.05μm, 1.3μm, 1.8μm, 2.1μm and 2.7μm have been observed in Li7La3-xNdxHf2O12:Ho3+ (x=0.00-0.15) under 808nm laser diode excitation. Near-infrared luminescence due to holmium ions with residual concentration in the Li7La3Hf2O12 host has been studied. The intensity of 2.1 and 2.7μm lines associated with 5I7→5I8 and 5I6→5I7 transitions in Ho3+ depends on the neodymium codopant concentration. This result indicates that Nd3+ ions can be potentially used as sensitizers for Ho3+ ions to stimulate the intense near-infrared emission in this system. Possible energy transfer mechanisms between lanthanide ions have been briefly discussed.}, }
@article {pmid28264524, year = {2017}, author = {Denisova, AV and Tibbelin, J and Emanuelsson, R and Ottosson, H}, title = {A Computational Investigation of the Substituent Effects on Geometric, Electronic, and Optical Properties of Siloles and 1,4-Disilacyclohexa-2,5-dienes.}, journal = {Molecules (Basel, Switzerland)}, volume = {22}, number = {3}, pages = {}, pmid = {28264524}, issn = {1420-3049}, mesh = {Electrons ; Models, Molecular ; Organosilicon Compounds/*chemistry ; Quantum Theory ; Silanes/*chemistry ; }, abstract = {Thirty two differently substituted siloles 1a-1p and 1,4-disilacyclohexa-2,5-dienes 2a-2p were investigated by quantum chemical calculations using the PBE0 hybrid density functional theory (DFT) method. The substituents included σ-electron donating and withdrawing, as well as π-electron donating and withdrawing groups, and their effects when placed at the Si atom(s) or at the C atoms were examined. Focus was placed on geometries, frontier orbital energies and the energies of the first allowed electronic excitations. We analyzed the variation in energies between the orbitals which correspond to HOMO and LUMO for the two parent species, here represented as ΔεHL, motivated by the fact that the first allowed transitions involve excitation between these orbitals. Even though ΔεHL and the excitation energies are lower for siloles than for 1,4-disilacyclohexa-2,5-dienes the latter display significantly larger variations with substitution. The ΔεHL of the siloles vary within 4.57-5.35 eV (ΔΔεHL = 0.78 eV) while for the 1,4-disilacyclohexa-2,5-dienes the range is 5.49-7.15 eV (ΔΔεHL = 1.66 eV). The excitation energy of the first allowed transitions display a moderate variation for siloles (3.60-4.41 eV) whereas the variation for 1,4-disilacyclohexa-2,5-dienes is nearly doubled (4.69-6.21 eV). Cyclobutadisiloles combine the characteristics of siloles and 1,4-disilacyclohexa-2,5-diene by having even lower excitation energies than siloles yet also extensive variation in excitation energies to substitution of 1,4-disilacyclohexa-2,5-dienes (3.47-4.77 eV, variation of 1.30 eV).}, }
@article {pmid28252610, year = {2017}, author = {Bogomolov, DV and Putintsev, VA and Zbrueva, YV and Denisova, OP}, title = {[Certain immunohistochemical markers of the intravitality of strangulation mechanical asphyxia].}, journal = {Sudebno-meditsinskaia ekspertiza}, volume = {60}, number = {1}, pages = {8-10}, doi = {10.17116/sudmed20176018-10}, pmid = {28252610}, issn = {0039-4521}, mesh = {*Asphyxia/diagnosis/etiology/pathology ; Biomarkers/analysis ; Fibrinogen/*analysis ; Forensic Pathology/methods ; Humans ; Immunohistochemistry ; *Lung/metabolism/pathology ; Neck/pathology ; *Neck Injuries/etiology/pathology ; *Skin/metabolism/pathology ; *Suicide ; }, abstract = {Thus article was designed to report the results of the investigations into specific histological features of the skin and soft tissue samples taken from the strangulation areas on the neck and from the lungs of the persons who had committed suicide by hanging. The studies revealed the well apparent expression of fibrinogen in the derma and the subdermal cellolocutaneous layer of the skin subjected to intravital strangulation. Similar changes were absent in lung alveoli.}, }
@article {pmid28239784, year = {2017}, author = {Bugaeva, LI and Denisova, TD and Sergeeva, SA and Morozova, YA and Kharlamov, IV}, title = {Effects of Afobazole on Postnatal Development of Rat Offspring.}, journal = {Bulletin of experimental biology and medicine}, volume = {162}, number = {4}, pages = {441-444}, doi = {10.1007/s10517-017-3635-z}, pmid = {28239784}, issn = {1573-8221}, mesh = {Animals ; Animals, Newborn ; Anti-Anxiety Agents/*adverse effects ; Avoidance Learning/drug effects ; Behavior, Animal/drug effects ; Benzimidazoles/*adverse effects ; Female ; Maternal Exposure/*adverse effects ; Maze Learning/drug effects ; Memory/drug effects ; Morpholines/*adverse effects ; Motor Activity/drug effects ; Muscle Strength/drug effects ; Muscle, Skeletal/drug effects/growth &amp; development ; Pregnancy ; Prenatal Exposure Delayed Effects/*etiology/physiopathology ; Rats ; Reflex, Pupillary/drug effects ; Sexual Maturation/drug effects ; Weight Gain/drug effects ; }, abstract = {Physical development, development of sensory and motor reflexes, behavioral and mnestic patterns were studied infantile and juvenile rat pups born by female rats receiving Afobazole during pregnancy. Physical development and development of sensory and motor reflexes in rats were completed without pathologies by the age of 2 months. During the infantile period, the rat pups demonstrated reduced body weight gain, delayed eye opening and pupillary response formation, decreased muscle force, and suppressed motor behavior. During the juvenile period, body weight gain and development of motor behavior were intensified. Females demonstrated later vagina opening and poorer mnestic responses. In males, the terms of sexual maturation were unchanged and processes of learning and memory retrieval were not impaired.}, }
@article {pmid28239517, year = {2017}, author = {Denisova, K and Zhao, G and Wang, Z and Goh, S and Huo, Y and Peterson, BS}, title = {Cortical interactions during the resolution of information processing demands in autism spectrum disorders.}, journal = {Brain and behavior}, volume = {7}, number = {2}, pages = {e00596}, pmid = {28239517}, issn = {2162-3279}, support = {R01 MH089582/MH/NIMH NIH HHS/United States ; T32 MH016434/MH/NIMH NIH HHS/United States ; }, mesh = {Adolescent ; Adult ; Attention/*physiology ; Autism Spectrum Disorder/*physiopathology ; Brain/*physiopathology ; Child ; Female ; Functional Neuroimaging/*methods ; Humans ; Magnetic Resonance Imaging ; Male ; Middle Aged ; Psychomotor Performance/*physiology ; Space Perception/*physiology ; Visual Perception/*physiology ; Young Adult ; }, abstract = {INTRODUCTION: Our flexible and adaptive interactions with the environment are guided by our individual representation of the physical world, estimated through sensation and evaluation of available information against prior knowledge. When linking sensory evidence with higher-level expectations for action, the central nervous system (CNS) in typically developing (TD) individuals relies in part on distributed and interacting cortical regions to communicate neuronal signals flexibly across the brain. Increasing evidence suggests that the balance between levels of signal and noise during information processing may be disrupted in individuals with Autism Spectrum Disorders (ASD).<br><br>METHODS: Participants with and without ASD performed a visuospatial interference task while undergoing functional Magnetic Resonance Imaging (fMRI). We empirically estimated parameters characterizing participants' latencies and their subtle fluctuations (noise accumulation) over the 16-min scan. We modeled hemodynamic activation and used seed-based analyses of neural coupling to study dysfunction in interference-specific connectivity in a subset of ASD participants who were nonparametrically matched to TD participants on age, male-to-female ratio, and magnitude of movement during the scan.<br><br>RESULTS: Stochastic patterns of response fluctuations reveal significantly higher noise-to-signal levels and a more random and noisy structure in ASD versus TD participants, and in particular ASD adults who have the greatest clinical autistic deficits. While individuals with ASD show an overall weaker modulation of interference-specific functional connectivity relative to TD individuals, in particular between the seeds of Anterior Cingulate Cortex (ACC) and Inferior Parietal Sulcus (IPS) and the rest of the brain, we found that in ASD, higher uncertainty during the task is linked to increased interference-specific coupling between bilateral anterior insula and prefrontal cortex.<br><br>CONCLUSIONS: Subtle and informative differences in the structure of experiencing information exist between ASD and TD individuals. Our findings reveal in ASD an atypical capacity to apply previously perceived information in a manner optimal for adaptive functioning, plausibly revealing suboptimal message-passing across the CNS.}, }
@article {pmid28233034, year = {2017}, author = {Tashi, T and Scott Reading, N and Wuren, T and Zhang, X and Moore, LG and Hu, H and Tang, F and Shestakova, A and Lorenzo, F and Burjanivova, T and Koul, P and Guchhait, P and Wittwer, CT and Julian, CG and Shah, B and Huff, CD and Gordeuk, VR and Prchal, JT and Ge, R}, title = {Gain-of-function EGLN1 prolyl hydroxylase (PHD2 D4E:C127S) in combination with EPAS1 (HIF-2α) polymorphism lowers hemoglobin concentration in Tibetan highlanders.}, journal = {Journal of molecular medicine (Berlin, Germany)}, volume = {95}, number = {6}, pages = {665-670}, pmid = {28233034}, issn = {1432-1440}, support = {P01 CA108671/CA/NCI NIH HHS/United States ; }, mesh = {Acclimatization/*genetics ; Adult ; Altitude ; Asian Continental Ancestry Group/*genetics ; Basic Helix-Loop-Helix Transcription Factors/*genetics ; Erythropoietin/blood ; Female ; Ferritins/blood ; Gene-Environment Interaction ; Haplotypes ; Hemoglobins/*analysis ; Humans ; Hypoxia-Inducible Factor-Proline Dioxygenases/*genetics ; Male ; Polymorphism, Single Nucleotide ; Tibet ; }, abstract = {Tibetans have lived at high altitude for generations and are thought to be genetically adapted to hypoxic environments. Most are protected from hypoxia-induced polycythemia, and a haplotype of EPAS1, encoding hypoxia-inducible factor (HIF-2α), has been associated with lower hemoglobin levels. We earlier reported a Tibetan-specific EGLN1 haplotype encoding PHD2 which abrogates HIF augmentation in hypoxia. We genotyped 347 Tibetan individuals from varying altitudes for both the Tibetan-specific EGLN1 haplotype and 10 candidate SNPs in the EPAS1 haplotype and correlated their association with hemoglobin levels. The effect of the EGLN1 haplotype on hemoglobin exhibited age dependency at low altitude, while at higher altitudes, it showed a trend to lower hemoglobin levels in the presence of the Tibetan-selected EPAS1 rs142764723 C/C allele. The observed gene-environment and gene-gene interactions and the moderate effect of the EGLN1 and EPAS1 haplotypes on hemoglobin indicate that other modifiers exist. It remains to be determined whether a blunting of erythropoiesis or other physiological consequences of HIF downregulation are the primary drivers of these genetic adaptations among Tibetans.<br><br>KEY MESSAGE: Most Tibetans are protected from polycythemia while living in high altitude. An EGLN1 co-adapted haplotype, EGLN1 c.12C>G, c.380G>C is uniquely Tibetan. The Tibetan EPAS1 haplotype has introgressed from the Denisovan genome. While EGLN1 and EPAS1 genotypes lower Hb, this study indicates additional Hb modifiers.}, }
@article {pmid28158547, year = {2016}, author = {Povysil, G and Hochreiter, S}, title = {IBD Sharing between Africans, Neandertals, and Denisovans.}, journal = {Genome biology and evolution}, volume = {8}, number = {12}, pages = {3406-3416}, pmid = {28158547}, issn = {1759-6653}, mesh = {Africa ; African Continental Ancestry Group/genetics ; Animals ; Asian Continental Ancestry Group/genetics ; Breeding ; DNA/genetics ; European Continental Ancestry Group/genetics ; *Evolution, Molecular ; *Gene Flow ; Genome ; Hominidae/*genetics ; Humans ; Neanderthals/genetics ; }, abstract = {Interbreeding between ancestors of humans and other hominins outside of Africa has been studied intensively, while their common history within Africa still lacks proper attention. However, shedding light on human evolution in this time period about which little is known, is essential for understanding subsequent events outside of Africa. We investigate the genetic relationships of humans, Neandertals, and Denisovans by identifying very short DNA segments in the 1000 Genomes Phase 3 data that these hominins share identical by descent (IBD). By focusing on low frequency and rare variants, we identify very short IBD segments with high confidence. These segments reveal events from a very distant past because shorter IBD segments are presumably older than longer ones. We extracted two types of very old IBD segments that are not only shared among humans, but also with Neandertals and/or Denisovans. The first type contains longer segments that are found primarily in Asians and Europeans where more segments are found in South Asians than in East Asians for both Neandertal and Denisovan. These longer segments indicate complex admixture events outside of Africa. The second type consists of shorter segments that are shared mainly by Africans and therefore may indicate events involving ancestors of humans and other ancient hominins within Africa. Our results from the autosomes are further supported by an analysis of chromosome X, on which segments that are shared by Africans and match the Neandertal and/or Denisovan genome were even more prominent. Our results indicate that interbreeding with other hominins was a common feature of human evolution starting already long before ancestors of modern humans left Africa.}, }
@article {pmid33473722, year = {2017}, author = {Druzhkova, AS and Makunin, AI and Vorobieva, NV and Vasiliev, SK and Ovodov, ND and Shunkov, MV and Trifonov, VA and Graphodatsky, AS}, title = {Complete mitochondrial genome of an extinct Equus (Sussemionus) ovodovi specimen from Denisova cave (Altai, Russia).}, journal = {Mitochondrial DNA. Part B, Resources}, volume = {2}, number = {1}, pages = {79-81}, pmid = {33473722}, issn = {2380-2359}, abstract = {Sussemionus is an extinct subgenus of Equus first characterized and delineated in 2010. The almost complete mitochondrial genome is available only for a single specimen of Sussemionus - a 40,000 years old E. ovodovi from Proskuryakova cave (Khakassia, Russia). Our studies of ancient horses from Denisova cave (Altai, Russia) revealed mitochondrial DNA of this species in a 32,000 years old sample. Using alignments to multiple mitochondrial genomes of non-caballine equids, we recovered 100% complete mitochondrial genome of E. ovodovi for the first time. Phylogenetic analysis demonstrates close relationship between this individual and the one previously described in Khakassia.}, }
@article {pmid28146472, year = {2017}, author = {Carus-Cadavieco, M and Gorbati, M and Ye, L and Bender, F and van der Veldt, S and Kosse, C and Börgers, C and Lee, SY and Ramakrishnan, C and Hu, Y and Denisova, N and Ramm, F and Volitaki, E and Burdakov, D and Deisseroth, K and Ponomarenko, A and Korotkova, T}, title = {Gamma oscillations organize top-down signalling to hypothalamus and enable food seeking.}, journal = {Nature}, volume = {542}, number = {7640}, pages = {232-236}, pmid = {28146472}, issn = {1476-4687}, support = {R01 NS067199/NS/NINDS NIH HHS/United States ; }, mesh = {Animals ; Eating/physiology/psychology ; Energy Metabolism/physiology ; Feeding Behavior/*physiology/psychology ; Gamma Rhythm/*physiology ; Hypothalamus/cytology/*physiology ; Learning ; Male ; Mice ; Mice, Inbred C57BL ; Neurons/physiology ; Reward ; Somatostatin/metabolism ; }, abstract = {Both humans and animals seek primary rewards in the environment, even when such rewards do not correspond to current physiological needs. An example of this is a dissociation between food-seeking behaviour and metabolic needs, a notoriously difficult-to-treat symptom of eating disorders. Feeding relies on distinct cell groups in the hypothalamus, the activity of which also changes in anticipation of feeding onset. The hypothalamus receives strong descending inputs from the lateral septum, which is connected, in turn, with cortical networks, but cognitive regulation of feeding-related behaviours is not yet understood. Cortical cognitive processing involves gamma oscillations, which support memory, attention, cognitive flexibility and sensory responses. These functions contribute crucially to feeding behaviour by unknown neural mechanisms. Here we show that coordinated gamma (30-90 Hz) oscillations in the lateral hypothalamus and upstream brain regions organize food-seeking behaviour in mice. Gamma-rhythmic input to the lateral hypothalamus from somatostatin-positive lateral septum cells evokes food approach without affecting food intake. Inhibitory inputs from the lateral septum enable separate signalling by lateral hypothalamus neurons according to their feeding-related activity, making them fire at distinct phases of the gamma oscillation. Upstream, medial prefrontal cortical projections provide gamma-rhythmic inputs to the lateral septum; these inputs are causally associated with improved performance in a food-rewarded learning task. Overall, our work identifies a top-down pathway that uses gamma synchronization to guide the activity of subcortical networks and to regulate feeding behaviour by dynamic reorganization of functional cell groups in the hypothalamus.}, }
@article {pmid28139571, year = {2016}, author = {Khabarova, EA and Denisova, NP and Rogov, DY and Dmitriev, AB}, title = {[The preliminary results of subthalamic nucleus stimulation after destructive surgery in Parkinson's disease].}, journal = {Zhurnal voprosy neirokhirurgii imeni N. N. Burdenko}, volume = {80}, number = {6}, pages = {36-41}, doi = {10.17116/neiro201680636-41}, pmid = {28139571}, issn = {0042-8817}, mesh = {Case-Control Studies ; Deep Brain Stimulation/*adverse effects ; Female ; Globus Pallidus/surgery ; Humans ; Male ; Middle Aged ; Motor Activity ; Neurosurgical Procedures/*adverse effects ; Parkinson Disease/surgery/*therapy ; Subthalamic Nucleus/*physiopathology ; Treatment Outcome ; }, abstract = {OBJECTIVE: To evaluate the efficacy of bilateral electrical stimulation (ES) of the subthalamic nucleus (STN) in patients with Parkinson's disease (PD) after preceding pallidotomy or ventrolateral (VL) thalamotomy.<br><br>MATERIAL AND METHODS: The study included 9 patients with bilateral STN ES who had undergone previous unilateral destructive surgery on the subcortical structures: pallidotomy (5 patients) and VL thalamotomy (4). A control group consisted of 9 patients with STN ES, without prior destructive surgery. A clinical and neurological examination included quantitative assessment of motor disturbances using the Hoehn-Yahr scale and Unified Parkinson's disease rating scale (UPDRS). UPDRS was used to evaluate the motor activity (IIIrd part of the scale) and severity of drug-induced dyskinesia and motor fluctuations (IVth part of the scale).<br><br>RESULTS: In the group of STN ES with preceding destruction of the subcortical structures, an improvement in motor functions in the early period (6 months) was 45%, and severity of drug-induced complications was decreased by 75%. In a group of STN DBS without destruction, motor disturbances were improved by 61%, and drug-induced complications were decreased by 77%. Improvement in motor functions amounted to 51.9% in patients with preceding pallidotomy (GPi destruction) and 37.5% in a group with preceding VL thalamotomy. The equivalent dose of levodopa was reduced by 51.39%, from 1,008±346 to 490±194, in the study group and by 55.04%, from 963±96 to 433±160, in the control group.<br><br>CONCLUSION: Bilateral STN neurostimulation is effective after unilateral stereotaxic destruction of the subcortical structures in PD patients.}, }
@article {pmid30615384, year = {2017}, author = {Kulyutsina, ER and Tatarchenko, IP and Levashova, OA and Denisova, AG and Drujinina, TA}, title = {[The interrelationship of indices of hemocysteine and genetic polymorphisms conditioning disorders of folates metabolism in healthy population].}, journal = {Klinicheskaia laboratornaia diagnostika}, volume = {62}, number = {2}, pages = {82-87}, pmid = {30615384}, issn = {0869-2084}, mesh = {5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase/*genetics ; Adult ; Alleles ; Female ; Ferredoxin-NADP Reductase/*genetics ; Folic Acid/genetics/metabolism ; Gene Frequency ; *Genetic Association Studies ; Genetic Predisposition to Disease ; Genotype ; Homocysteine/blood/genetics ; Humans ; Male ; Methylenetetrahydrofolate Reductase (NADPH2)/*genetics ; Middle Aged ; Risk Factors ; }, abstract = {The study was carried out to investigate relationship of the level of homocysteine and genetic polymorphisms conditioning disorders of metabolism of folates in healthy population and of various age and gender groups. The study covered 168 donors: 98 males and 70 females. Two gender groups were singled out and in each of them age groups: 18-31, 32-45 and 46-60 years old. The analysis of concentration of homocysteine was implemented using immune chemiluminescence analysis. The polymerase chain reaction was applied for analyzing genetic polymorphisms associated with disorders of folate cycle by genes MTHFR (met hylentetrahydrofolatereductase) (polymorphisms MTHFR: 677 C>T and MTHFR: 1298 A>C), MTR (B12-dependent methionine-synthetase) (polymorphism MTR: 2756 A>G) and MTRR (methionine-synthetase-reductase) (polymorphism MTRR: 66 A>G). The level of homocysteine in blood of donors was reliably higher in male groups of 18-31 and 32-45 years old as against female groups comparable by age. The study established higher rate of occurrence of heterozygous genotypes bringing unfavorable types of polymorphisms of MTHFR and MTRR genes both in male and female groups. The study established higher rate of occurrence of unfavorable genotypes in examined patients in comparison with publications' data. The established statistically significant inverse correlation of concentration of homocysteine and genetic polymorphisms associated with disorders of folate cycle in individuals of young and middle age mainly males, permits to draw a conclusion about absence of their direct relationship. Therefore, implementation of genetic liability to increasing of homocysteine can occur under effect of external unfavorable factors.}, }
@article {pmid29874000, year = {2017}, author = {Bugaeva, LI and Denisova, TD and Mazanova, YA and Kharlamov, IV and Sergeeva, SA}, title = {[PHYSICAL AND BEHAVIORAL DEVELOPMENT OF THE OFFSPRING OF FEMALE RATS TREATED WITH AFOBAZOLE DURING PREGNANCY.].}, journal = {Eksperimental'naia i klinicheskaia farmakologiia}, volume = {80}, number = {1}, pages = {28-31}, pmid = {29874000}, issn = {0869-2092}, mesh = {Animals ; Animals, Newborn ; Anti-Anxiety Agents/*pharmacology ; Behavior, Animal/*drug effects ; Benzimidazoles/*pharmacology ; Female ; Lactation/*drug effects/physiology ; Male ; Morpholines/*pharmacology ; Motor Activity/*drug effects ; Pregnancy ; Prenatal Exposure Delayed Effects/diagnosis/pathology/physiopathology ; Sex Factors ; }, abstract = {It is experimentally established that afobazole produces no damaging action on the organogenesis and fetogenic processes registered in the postnatal period of rat offspring development. It was noted that, in rat babies in lactation age, the dynamics of body weight gain was lower on average by 7.4% (p < 0.05) in males and 17.0% (p < 0.001) in females; the rate of muscular force maturing was lower by 2.7% (p < 0.05); and the locomotive activity was lower (by 19.4% for ver- tical standings and by 50% for looking into floor holes, p < 0.05) compared to control values. For the same offspring passed to definitive food, the body weight gain and behavioral activity did not differ from control indicators, while the terms of sexual development were delayed in females and did not change in males. By two-month age, the physical development of rat offspring was completely created and met physiological standards.}, }
@article {pmid28007980, year = {2017}, author = {Racimo, F and Gokhman, D and Fumagalli, M and Ko, A and Hansen, T and Moltke, I and Albrechtsen, A and Carmel, L and Huerta-Sánchez, E and Nielsen, R}, title = {Archaic Adaptive Introgression in TBX15/WARS2.}, journal = {Molecular biology and evolution}, volume = {34}, number = {3}, pages = {509-524}, pmid = {28007980}, issn = {1537-1719}, support = {R01 HG003229/HG/NHGRI NIH HHS/United States ; }, mesh = {Adaptation, Biological/*genetics ; Adipose Tissue/physiology ; Alleles ; Animals ; DNA Methylation ; DNA, Ancient ; Greenland ; Haplotypes ; Humans ; Inuits/*genetics ; Models, Genetic ; Neanderthals ; Polymorphism, Single Nucleotide ; Selection, Genetic ; Sequence Analysis, DNA/methods ; T-Box Domain Proteins/*genetics ; }, abstract = {A recent study conducted the first genome-wide scan for selection in Inuit from Greenland using single nucleotide polymorphism chip data. Here, we report that selection in the region with the second most extreme signal of positive selection in Greenlandic Inuit favored a deeply divergent haplotype that is closely related to the sequence in the Denisovan genome, and was likely introgressed from an archaic population. The region contains two genes, WARS2 and TBX15, and has previously been associated with adipose tissue differentiation and body-fat distribution in humans. We show that the adaptively introgressed allele has been under selection in a much larger geographic region than just Greenland. Furthermore, it is associated with changes in expression of WARS2 and TBX15 in multiple tissues including the adrenal gland and subcutaneous adipose tissue, and with regional DNA methylation changes in TBX15.}, }
@article {pmid27919236, year = {2016}, author = {Pajic, P and Lin, YL and Xu, D and Gokcumen, O}, title = {The psoriasis-associated deletion of late cornified envelope genes LCE3B and LCE3C has been maintained under balancing selection since Human Denisovan divergence.}, journal = {BMC evolutionary biology}, volume = {16}, number = {1}, pages = {265}, pmid = {27919236}, issn = {1471-2148}, mesh = {Alleles ; Cornified Envelope Proline-Rich Proteins/*genetics ; *Evolution, Molecular ; *Gene Deletion ; Gene Frequency ; Genetic Predisposition to Disease ; Humans ; Polymorphism, Single Nucleotide ; Psoriasis/*genetics ; Sequence Deletion ; }, abstract = {BACKGROUND: A common, 32kb deletion of LCE3B and LCE3C genes is strongly associated with psoriasis. We recently found that this deletion is ancient, predating Human-Denisovan divergence. However, it was not clear why negative selection has not removed this deletion from the population.<br><br>RESULTS: Here, we show that the haplotype block that harbors the deletion (i) retains high allele frequency among extant and ancient human populations; (ii) harbors unusually high nucleotide variation (π, P < 4.1 × 10-3); (iii) contains an excess of intermediate frequency variants (Tajima's D, P < 3.9 × 10-3); and (iv) has an unusually long time to coalescence to the most recent common ancestor (TSel, 0.1 quantile).<br><br>CONCLUSIONS: Our results are most parsimonious with the scenario where the LCE3BC deletion has evolved under balancing selection in humans. More broadly, this is consistent with the hypothesis that a balance between autoimmunity and natural vaccination through increased exposure to pathogens maintains this deletion in humans.}, }
@article {pmid27916748, year = {2017}, author = {Noe, F and Polster, J and Geithe, C and Kotthoff, M and Schieberle, P and Krautwurst, D}, title = {OR2M3: A Highly Specific and Narrowly Tuned Human Odorant Receptor for the Sensitive Detection of Onion Key Food Odorant 3-Mercapto-2-methylpentan-1-ol.}, journal = {Chemical senses}, volume = {42}, number = {3}, pages = {195-210}, doi = {10.1093/chemse/bjw118}, pmid = {27916748}, issn = {1464-3553}, mesh = {Cells, Cultured ; Humans ; Molecular Structure ; Odorants/*analysis ; Onions/*chemistry ; Pentanols/*analysis/chemistry ; Receptors, Odorant/*metabolism ; *Smell ; Sulfhydryl Compounds/*analysis/chemistry ; }, abstract = {The detection of key food odorants appears to be an important capability of odorant receptors. Here, thiols occupy an outstanding position among the 230 known key food odorants because of their very low odor thresholds. Members of the homologous series of 3-mercapto-2-methylalkan-1-ols have been described as onion key food odorants or food constituents and are detected at logarithmically different thresholds. 3-Mercapto-2-methylpentan-1-ol being the only key food odorant within this series also has the lowest odor threshold. Most odorants typically activate combinations of odorant receptors, which may be narrowly or broadly tuned. Consequently, a specific receptor activation pattern will define an odor quality. In contrast, here we show that just 1 of the 391 human odorant receptors, OR2M3, responded exclusively to 3-mercapto-2-methylpentan-1-ol of the 190 key food odorants tested, with a half maximal effective concentration at submicromolar concentration. Moreover, neither the Denisovan OR2M3 nor the closest OR2M3 homologs from five species did respond to this compound. This outstanding specificity of extremely narrowly tuned human OR2M3 can explain both odor qualities and odor threshold trend within a homologous series of 3-mercapto-2-methylalkan-1-ols and suggests a modern human-specific, food-related function of OR2M3 in detecting a single onion key food odorant.}, }
@article {pmid27869148, year = {2016}, author = {Torres, EB and Denisova, K}, title = {Motor noise is rich signal in autism research and pharmacological treatments.}, journal = {Scientific reports}, volume = {6}, number = {}, pages = {37422}, pmid = {27869148}, issn = {2045-2322}, mesh = {Adolescent ; Autistic Disorder/diagnosis/*drug therapy/*physiopathology ; Comorbidity ; Female ; Head Movements/drug effects ; Humans ; Intelligence ; Male ; Mental Health ; *Motor Activity/drug effects ; Noise ; Precision Medicine ; Psychotropic Drugs/pharmacology/therapeutic use ; Stochastic Processes ; }, abstract = {The human body is in constant motion, from every breath that we take, to every visibly purposeful action that we perform. Remaining completely still on command is a major achievement as involuntary fluctuations in our motions are difficult to keep under control. Here we examine the noise-to-signal ratio of micro-movements present in time-series of head motions extracted from resting-state functional magnetic resonance imaging scans in 1048 participants. These included individuals with autism spectrum disorders (ASD) and healthy-controls in shared data from the Autism Brain Imaging Data Exchange (ABIDE) and the Attention-Deficit Hyperactivity Disorder (ADHD-200) databases. We find excess noise and randomness in the ASD cases, suggesting an uncertain motor-feedback signal. A power-law emerged describing an orderly relation between the dispersion and shape of the probability distribution functions best describing the stochastic properties under consideration with respect to intelligence quotient (IQ-scores). In ASD, deleterious patterns of noise are consistently exacerbated with the presence of secondary (comorbid) neuropsychiatric diagnoses, lower verbal and performance intelligence, and autism severity. Importantly, such patterns in ASD are present whether or not the participant takes psychotropic medication. These data unambiguously establish specific noise-to-signal levels of head micro-movements as a biologically informed core feature of ASD.}, }
@article {pmid27839976, year = {2016}, author = {Gittelman, RM and Schraiber, JG and Vernot, B and Mikacenic, C and Wurfel, MM and Akey, JM}, title = {Archaic Hominin Admixture Facilitated Adaptation to Out-of-Africa Environments.}, journal = {Current biology : CB}, volume = {26}, number = {24}, pages = {3375-3382}, pmid = {27839976}, issn = {1879-0445}, support = {R01 GM110068/GM/NIGMS NIH HHS/United States ; }, mesh = {Adaptation, Physiological/*genetics ; Animals ; *Biological Evolution ; DNA/*genetics ; Demography ; Gene Expression Regulation ; Genetic Variation ; *Genome, Human/genetics ; Haplotypes ; Humans ; Neanderthals ; Pigmentation ; }, abstract = {As modern humans dispersed from Africa throughout the world, they encountered and interbred with archaic hominins, including Neanderthals and Denisovans [1, 2]. Although genome-scale maps of introgressed sequences have been constructed [3-6], considerable gaps in knowledge remain about the functional, phenotypic, and evolutionary significance of archaic hominin DNA that persists in present-day individuals. Here, we describe a comprehensive set of analyses that identified 126 high-frequency archaic haplotypes as putative targets of adaptive introgression in geographically diverse populations. These loci are enriched for immune-related genes (such as OAS1/2/3, TLR1/6/10, and TNFAIP3) and also encompass genes (including OCA2 and BNC2) that influence skin pigmentation phenotypes. Furthermore, we leveraged existing and novel large-scale gene expression datasets to show many positively selected archaic haplotypes act as expression quantitative trait loci (eQTLs), suggesting that modulation of transcript abundance was a common mechanism facilitating adaptive introgression. Our results demonstrate that hybridization between modern and archaic hominins provided an important reservoir of advantageous alleles that enabled adaptation to out-of-Africa environments.}, }
@article {pmid27633826, year = {2016}, author = {Birolo, C and Zannin, ME and Arsenyeva, S and Cimaz, R and Miserocchi, E and Dubko, M and Deslandre, CJ and Falcini, F and Alessio, M and La Torre, F and Denisova, E and Martini, G and Nikishina, I and Zulian, F}, title = {Comparable Efficacy of Abatacept Used as First-line or Second-line Biological Agent for Severe Juvenile Idiopathic Arthritis-related Uveitis.}, journal = {The Journal of rheumatology}, volume = {43}, number = {11}, pages = {2068-2073}, doi = {10.3899/jrheum.151389}, pmid = {27633826}, issn = {0315-162X}, mesh = {Abatacept/*therapeutic use ; Adolescent ; Antirheumatic Agents/*therapeutic use ; Arthritis, Juvenile/*drug therapy ; Child ; Child, Preschool ; Female ; Humans ; Immunosuppressive Agents/*therapeutic use ; Male ; Retreatment ; Treatment Outcome ; Uveitis/*drug therapy ; Young Adult ; }, abstract = {OBJECTIVE: Abatacept (ABA) has recently been proposed as second-line treatment in patients with juvenile idiopathic arthritis (JIA)-associated uveitis refractory to anti-tumor necrosis factor-α (anti-TNF) agents, but little is known about its efficacy as a first-line approach. The aim of the present study was to compare the safety and efficacy of ABA as a first-line biological agent (ABA-1) with that of ABA as a second-line treatment after 1 or more anti-TNF agents (ABA-2), in patients with severe JIA-related uveitis.<br><br>METHODS: In this multicenter study, we collected data on patients with severe JIA-related uveitis treated with ABA as a first-line or second-line biological agent. Changes in frequency of uveitis flares/year and ocular complications before and after ABA treatment, clinical remission, and side effects were recorded.<br><br>RESULTS: Thirty-five patients with a mean age of 10.8 years were treated with ABA for a mean period of 19.6 months. In 4 patients, ABA administration was discontinued, owing to inefficacy on arthritis in 3 cases and allergic reaction in 1. Thirty-one patients, 14 in the ABA-1 group and 17 in the ABA-2 group, completed the 12-month followup period; of these, 17 (54.8%) had clinical remission. The mean frequency of uveitis flares decreased from 4.1 to 1.2 in the ABA-1 group (p = 0.002) and from 3.7 to 1.2 in the ABA-2 group (p = 0.004). Preexisting ocular complications improved or remained stable in all but 5 patients, all in the ABA-2 group. No significant difference was found between the efficacy of the 2 treatment modalities. ABA confirmed its good safety profile.<br><br>CONCLUSION: ABA, used as first-line biological treatment or after 1 or more anti-TNF agents, induces a comparable improvement in severe refractory JIA-related uveitis.}, }
@article {pmid27801420, year = {2016}, author = {Voevoda, MI and Kovalkova, NA and Ragino, YI and Travnikova, NY and Denisova, DV}, title = {[Prevalence of metabolic syndrome in 25-45-year-old Novosibirsk dwellers].}, journal = {Terapevticheskii arkhiv}, volume = {88}, number = {10}, pages = {51-56}, doi = {10.17116/terarkh2016881051-56}, pmid = {27801420}, issn = {0040-3660}, mesh = {Adult ; Age Factors ; Anthropometry/methods ; Blood Glucose/*analysis ; *Blood Pressure Determination/methods/statistics &amp; numerical data ; Cross-Sectional Studies ; Female ; Humans ; Lipoproteins, HDL/blood ; Lipoproteins, LDL/blood ; Male ; *Metabolic Syndrome/blood/diagnosis/epidemiology ; Prevalence ; Risk Factors ; Sex Factors ; Siberia/epidemiology ; Triglycerides/blood ; }, abstract = {AIM: To study the prevalence of metabolic syndrome (MS) and its components in a 25-45-year-old Novosibirsk population.<br><br>SUBJECTS AND METHODS: The Novosibirsk Research Institute of Internal and Preventive Medicine conducted a cross-sectional population-based survey in one of the typical districts of Novosibirsk in 2013-2015. The survey covered 346 men and 408 women. The criteria of the National Cholesterol Education Program Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (NCEP-ATP III, 2001), the International Diabetes Federation (IDF, 2005), the Joint Interim Statement (JIS, 2009), and the All-Russian Research Society of Cardiologists (ARRSC, 2009) were used to detect MS. According to the ARRSC criteria, MS was recorded if a waist circumference (WC) was >80 cm for women and >94 cm for men in conjunction with 2 of the following criteria: a blood pressure ≥130/85 mm Hg, triglycerides (TG) ≥1.7 mmol/l, high-density lipoprotein (HDL) cholesterol <1.0 mmol/l for men and <1.2 mmol/l for women, low-density lipoprotein (LDL) cholesterol >3.0 mmol/l, and plasma glucose level ≥6.1 mmol/l.<br><br>RESULTS: According to the 2009 ARRSC criteria, the prevalence of abdominal obesity, hypertension, hypertriglyceridemia, HDL hypocholestrolemia, LDL hypercholesterolemia, and high plasma glucose level was 42.6, 33.5, 17.5, 24.3, 64.8, and 29%, respectively. The prevalence of MS in 25-45-year-old Novosibirsk people was 17% (19.9% in men and 14.5% in women) according to the 2001 NCEP-ATP III, 27% (29.5% in men and 24.5% in women) according to the 2005 IDF criteria, 30% (35.8% in men and 25% in women) according to the 2009 JIS criteria, and 29.3% (33.2% in men and 26% in women) according to the 2009 ARRSC criteria, this was higher among men than women. There was an increase in the prevalence of MS with age.<br><br>CONCLUSION: The highest prevalence of MS was 30 and 29.3% when using the 2009 JIS and the 2009 ARRSC criteria, respectively. Whatever the criterion was used, MS was more frequently recorded in men than women.}, }
@article {pmid27154591, year = {2016}, author = {Kozela, M and Bobak, M and Besala, A and Micek, A and Kubinova, R and Malyutina, S and Denisova, D and Richards, M and Pikhart, H and Peasey, A and Marmot, M and Pająk, A}, title = {The association of depressive symptoms with cardiovascular and all-cause mortality in Central and Eastern Europe: Prospective results of the HAPIEE study.}, journal = {European journal of preventive cardiology}, volume = {23}, number = {17}, pages = {1839-1847}, pmid = {27154591}, issn = {2047-4881}, support = {G19/35//Medical Research Council/United Kingdom ; G0100222//Medical Research Council/United Kingdom ; G8802774//Medical Research Council/United Kingdom ; MC_UU_12019/3//Medical Research Council/United Kingdom ; G0902037//Medical Research Council/United Kingdom ; G1000616//Medical Research Council/United Kingdom ; G0701830//Medical Research Council/United Kingdom ; G0601647//Medical Research Council/United Kingdom ; RG/07/008/23674//British Heart Foundation/United Kingdom ; R01 AG023522/AG/NIA NIH HHS/United States ; WT081081//Wellcome Trust/United Kingdom ; }, mesh = {Aged ; Cardiovascular Diseases/etiology/*mortality ; Cause of Death/trends ; Depressive Disorder/*complications/epidemiology ; Europe/epidemiology ; Female ; Follow-Up Studies ; Humans ; Incidence ; Male ; Middle Aged ; Prospective Studies ; *Registries ; *Risk Assessment ; Risk Factors ; Socioeconomic Factors ; Survival Rate/trends ; Time Factors ; }, abstract = {BACKGROUND: Studies in western populations have shown a positive association between depression and cardiovascular disease (CVD) and all-cause mortality. The association with depressive symptoms seems to be graded, rather than limited to the presence versus the absence of depression. Evidence from populations with potentially different patterns of confounders helps to address the consistency of these findings. The objective of the study was to investigate the association between depressive symptoms and all-cause and CVD mortality in populations of Central and Eastern Europe.<br><br>STUDY DESIGN: This was a prospective cohort study.<br><br>METHODS: A total of 24,542 participants aged 45-69 years, randomly selected from populations of Novosibirsk (Russia), Krakow (Poland) and six Czech towns, were included. Depressive symptoms, assessed by the 20-item Center for Epidemiologic Studies Depression (CES-D) scale, were used as both continuous and categorical variables. Data on deaths were obtained from local or national death registers. Associations between depression and mortality were assessed using Cox proportional hazards models.<br><br>RESULTS: Over a median of 7 years, 2091 deaths from all causes and 850 CVD deaths occurred in the cohorts. There was a graded association between CES-D score and mortality; the hazard ratio (HR) of CVD mortality for a 1 SD increase in CES-D was 1.20 (95% confidence interval (CI): 1.16-1.24) in men and 1.23 (95% CI: 1.12-1.35) in women; for all-cause mortality, the HRs were 1.13 (95% CI: 1.09-1.18) and 1.17 (95% CI: 1.10-1.25), respectively. The results were similar across countries.<br><br>CONCLUSIONS: Depressive symptoms predicted CVD and all-cause mortality independently of a wide range of potential confounders. The association followed a gradient and increased mortality risks were associated with scores below the cut-offs that are commonly used to define 'depression'.}, }
@article {pmid27756828, year = {2017}, author = {Racimo, F and Marnetto, D and Huerta-Sánchez, E}, title = {Signatures of Archaic Adaptive Introgression in Present-Day Human Populations.}, journal = {Molecular biology and evolution}, volume = {34}, number = {2}, pages = {296-317}, pmid = {27756828}, issn = {1537-1719}, support = {R01 HG003229/HG/NHGRI NIH HHS/United States ; }, mesh = {Adaptation, Biological/*genetics ; Alleles ; Animals ; Biological Evolution ; Computer Simulation ; DNA, Ancient/*analysis ; Databases, Nucleic Acid ; Evolution, Molecular ; Gene Frequency ; Genetics, Population ; Haplotypes ; Humans ; Neanderthals ; Phylogeny ; Selection, Genetic ; Sequence Analysis, DNA/*methods ; }, abstract = {Comparisons of DNA from archaic and modern humans show that these groups interbred, and in some cases received an evolutionary advantage from doing so. This process-adaptive introgression-may lead to a faster rate of adaptation than is predicted from models with mutation and selection alone. Within the last couple of years, a series of studies have identified regions of the genome that are likely examples of adaptive introgression. In many cases, once a region was ascertained as being introgressed, commonly used statistics based on both haplotype as well as allele frequency information were employed to test for positive selection. Introgression by itself, however, changes both the haplotype structure and the distribution of allele frequencies, thus confounding traditional tests for detecting positive selection. Therefore, patterns generated by introgression alone may lead to false inferences of positive selection. Here we explore models involving both introgression and positive selection to investigate the behavior of various statistics under adaptive introgression. In particular, we find that the number and allelic frequencies of sites that are uniquely shared between archaic humans and specific present-day populations are particularly useful for detecting adaptive introgression. We then examine the 1000 Genomes dataset to characterize the landscape of uniquely shared archaic alleles in human populations. Finally, we identify regions that were likely subject to adaptive introgression and discuss some of the most promising candidate genes located in these regions.}, }
@article {pmid27708712, year = {2016}, author = {Stankiewicz, P}, title = {One pedigree we all may have come from - did Adam and Eve have the chromosome 2 fusion?.}, journal = {Molecular cytogenetics}, volume = {9}, number = {}, pages = {72}, pmid = {27708712}, issn = {1755-8166}, abstract = {BACKGROUND: In contrast to Great Apes, who have 48 chromosomes, modern humans and likely Neandertals and Denisovans have and had, respectively, 46 chromosomes. The reduction in chromosome number was caused by the head-to-head fusion of two ancestral chromosomes to form human chromosome 2 (HSA2) and may have contributed to the reproductive barrier with Great Apes.<br><br>RESULTS: Next generation sequencing and molecular clock analyses estimated that this fusion arose prior to our last common ancestor with Neandertal and Denisovan hominins ~ 0.74 - 4.5 million years ago.<br><br>HYPOTHESES: I propose that, unlike recurrent Robertsonian translocations in humans, the HSA2 fusion was a single nonrecurrent event that spread through a small polygamous clan population bottleneck. Its heterozygous to homozygous conversion, fixation, and accumulation in the succeeding populations was likely facilitated by an evolutionary advantage through the genomic loss rather than deregulation of expression of the gene(s) flanking the HSA2 fusion site at 2q13.<br><br>CONCLUSIONS: The origin of HSA2 might have been a critical evolutionary event influencing higher cognitive functions in various early subspecies of hominins. Next generation sequencing of Homo heidelbergensis and Homo erectus genomes and complete reconstruction of DNA sequence of the orthologous subtelomeric chromosomes in Great Apes should enable more precise timing of HSA2 formation and better understanding of its evolutionary consequences.}, }
@article {pmid27708336, year = {2016}, author = {Papadakis, R and Li, H and Bergman, J and Lundstedt, A and Jorner, K and Ayub, R and Haldar, S and Jahn, BO and Denisova, A and Zietz, B and Lindh, R and Sanyal, B and Grennberg, H and Leifer, K and Ottosson, H}, title = {Metal-free photochemical silylations and transfer hydrogenations of benzenoid hydrocarbons and graphene.}, journal = {Nature communications}, volume = {7}, number = {}, pages = {12962}, pmid = {27708336}, issn = {2041-1723}, abstract = {The first hydrogenation step of benzene, which is endergonic in the electronic ground state (S0), becomes exergonic in the first triplet state (T1). This is in line with Baird's rule, which tells that benzene is antiaromatic and destabilized in its T1 state and also in its first singlet excited state (S1), opposite to S0, where it is aromatic and remarkably unreactive. Here we utilized this feature to show that benzene and several polycyclic aromatic hydrocarbons (PAHs) to various extents undergo metal-free photochemical (hydro)silylations and transfer-hydrogenations at mild conditions, with the highest yield for naphthalene (photosilylation: 21%). Quantum chemical computations reveal that T1-state benzene is excellent at H-atom abstraction, while cyclooctatetraene, aromatic in the T1 and S1 states according to Baird's rule, is unreactive. Remarkably, also CVD-graphene on SiO2 is efficiently transfer-photohydrogenated using formic acid/water mixtures together with white light or solar irradiation under metal-free conditions.}, }
@article {pmid27695917, year = {2017}, author = {Yasukochi, Y and Ohashi, J}, title = {Elucidating the origin of HLA-B*73 allelic lineage: Did modern humans benefit by archaic introgression?.}, journal = {Immunogenetics}, volume = {69}, number = {1}, pages = {63-67}, pmid = {27695917}, issn = {1432-1211}, mesh = {African Continental Ancestry Group/genetics ; Alleles ; Animals ; Asian Continental Ancestry Group/genetics ; Europe ; *Evolution, Molecular ; *Genome, Human ; HLA-B Antigens/*genetics ; Haplotypes/*genetics ; Hominidae/classification/*genetics ; Humans ; Neanderthals/genetics ; Pan troglodytes/genetics ; Phylogeny ; }, abstract = {A previous study reported that some of the human leukocyte antigen (HLA) alleles and haplotypes in present-day humans were acquired by admixture with archaic humans; specifically, an exceptionally diverged HLA-B*73 allele was proposed to be transmitted from Denisovans, although the DNA sequence of HLA-B*73 has not been detected in the Denisovan genome. Here, we argue against the hypothesis that HLA-B*73 introgressed from Denisovans into early modern humans. A phylogenetic analysis revealed that HLA-B*73:01 formed a monophyletic group with a chimpanzee MHC-B allele, strongly suggesting that the HLA-B*73 allelic lineage has been maintained in humans as well as in chimpanzees since the divergence of humans and chimpanzees. The global distribution of HLA-B*73 allele showed that the population frequency of HLA-B*73 in west Asia (0.24 %)-a possible site of admixture with Denisovans-is lower than that in Europe (0.72 %) and in south Asia (0.69 %). Furthermore, HLA-B*73 is not observed in Melanesia even though the Melanesian genome contains the highest proportion of Denisovan ancestry in present-day human populations. Single nucleotide polymorphisms in HLA-A*11-HLA-C*12:02 or HLA-A*11-C*15 haplotypes, one of which was assumed to be transmitted together with HLA-B*73 from Denisovans by the study of Abi-Rached and colleagues, were not differentiated from those in other HLA-A-C haplotypes in modern humans. These results do not support the introgression hypothesis. Thus, we conclude that it is highly likely that HLA-B*73 allelic lineage has been maintained in the direct ancestors of modern humans.}, }
@article {pmid27671680, year = {2016}, author = {Kaur, A and Denisova, OV and Qiao, X and Jumppanen, M and Peuhu, E and Ahmed, SU and Raheem, O and Haapasalo, H and Eriksson, J and Chalmers, AJ and Laakkonen, P and Westermarck, J}, title = {PP2A Inhibitor PME-1 Drives Kinase Inhibitor Resistance in Glioma Cells.}, journal = {Cancer research}, volume = {76}, number = {23}, pages = {7001-7011}, doi = {10.1158/0008-5472.CAN-16-1134}, pmid = {27671680}, issn = {1538-7445}, support = {G0802755//Medical Research Council/United Kingdom ; }, mesh = {Animals ; Glioma/*drug therapy/pathology ; Humans ; Mice ; Protein Kinase Inhibitors/*therapeutic use ; Protein Phosphatase 2/*metabolism ; Transfection ; }, abstract = {Glioblastoma multiforme lacks effective therapy options. Although deregulated kinase pathways are drivers of malignant progression in glioblastoma multiforme, glioma cells exhibit intrinsic resistance toward many kinase inhibitors, and the molecular basis of this resistance remains poorly understood. Here, we show that overexpression of the protein phosphatase 2A (PP2A) inhibitor protein PME-1 drives resistance of glioma cells to various multikinase inhibitors. The PME-1-elicited resistance was dependent on specific PP2A complexes and was mediated by a decrease in cytoplasmic HDAC4 activity. Importantly, both PME-1 and HDAC4 associated with human glioma progression, supporting clinical relevance of the identified mechanism. Synthetic lethality induced by both PME-1 and HDAC4 inhibition was dependent on the coexpression of proapoptotic protein BAD. Thus, PME-1-mediated PP2A inhibition is a novel mechanistic explanation for multikinase inhibitor resistance in glioma cells. Clinically, these results may inform patient stratification strategies for future clinical trials with selected kinase inhibitors in glioblastoma multiforme. Cancer Res; 76(23); 7001-11. ©2016 AACR.}, }
@article {pmid27662059, year = {2016}, author = {Wall, JD and Yoshihara Caldeira Brandt, D}, title = {Archaic admixture in human history.}, journal = {Current opinion in genetics &amp; development}, volume = {41}, number = {}, pages = {93-97}, doi = {10.1016/j.gde.2016.07.002}, pmid = {27662059}, issn = {1879-0380}, mesh = {Africa ; Animals ; DNA, Ancient/*analysis ; Genetics, Population/*history ; History, Ancient ; Hominidae/*genetics ; Humans ; Neanderthals/*genetics ; }, abstract = {Modern humans evolved in Southern or Eastern Africa, and spread from there across the rest of the world. As they expanded across Africa and Eurasia, they encountered other hominin groups. The extent to which modern and 'archaic' human groups interbred is an area of active research, and while we know that modern humans interbred with Neanderthals and Denisovans, there is not yet agreement on how many admixture events there were or on how much Neanderthal or Denisovan DNA can be found in contemporary genomes. Here we review what is known about archaic admixture in human history, with a focus on what has been discovered in the past 2 years.}, }
@article {pmid27655273, year = {2016}, author = {Aarts, JM and Alink, GM and Scherjon, F and MacDonald, K and Smith, AC and Nijveen, H and Roebroeks, W}, title = {Fire Usage and Ancient Hominin Detoxification Genes: Protective Ancestral Variants Dominate While Additional Derived Risk Variants Appear in Modern Humans.}, journal = {PloS one}, volume = {11}, number = {9}, pages = {e0161102}, pmid = {27655273}, issn = {1932-6203}, abstract = {Studies of the defence capacity of ancient hominins against toxic substances may contribute importantly to the reconstruction of their niche, including their diets and use of fire. Fire usage implies frequent exposure to hazardous compounds from smoke and heated food, known to affect general health and fertility, probably resulting in genetic selection for improved detoxification. To investigate whether such genetic selection occurred, we investigated the alleles in Neanderthals, Denisovans and modern humans at gene polymorphisms well-known to be relevant from modern human epidemiological studies of habitual tobacco smoke exposure and mechanistic evidence. We compared these with the alleles in chimpanzees and gorillas. Neanderthal and Denisovan hominins predominantly possess gene variants conferring increased resistance to these toxic compounds. Surprisingly, we observed the same in chimpanzees and gorillas, implying that less efficient variants are derived and mainly evolved in modern humans. Less efficient variants are observable from the first early Upper Palaeolithic hunter-gatherers onwards. While not clarifying the deep history of fire use, our results highlight the long-term stability of the genes under consideration despite major changes in the hominin dietary niche. Specifically for detoxification gene variants characterised as deleterious by epidemiological studies, our results confirm the predominantly recent appearance reported for deleterious human gene variants, suggesting substantial impact of recent human population history, including pre-Holocene expansions.}, }
@article {pmid30592826, year = {2016}, author = {Bulavin, VV and Chaplyuk, AL and Kal'manov, AS and Solomka, AV and Denisova, MP and Blinov, VV}, title = {[Modern methods of diagnosis of chronic viral hepatitis used in the practice of the military-medical examination].}, journal = {Voenno-meditsinskii zhurnal}, volume = {337}, number = {9}, pages = {10-17}, pmid = {30592826}, issn = {0026-9050}, mesh = {Chronic Disease ; Female ; Hepatitis, Viral, Human/*classification/*diagnosis ; Humans ; Male ; Military Medicine/*methods ; }, abstract = {Modern methods of diagnosis of chronic viral hepatitis used in the practice of the military-medical examination. The article presents current views on the classification of chronic viral hepatitis. The characteristic of methodical diagnostic approaches is presented: The attention to the etiological pathology verification is paid. The results of study on clinical characteristics of patients with chronic viral hepatitis are used as the basis for the developed criteria for evaluating fitness for military service.}, }
@article {pmid27569548, year = {2016}, author = {Lu, D and Lou, H and Yuan, K and Wang, X and Wang, Y and Zhang, C and Lu, Y and Yang, X and Deng, L and Zhou, Y and Feng, Q and Hu, Y and Ding, Q and Yang, Y and Li, S and Jin, L and Guan, Y and Su, B and Kang, L and Xu, S}, title = {Ancestral Origins and Genetic History of Tibetan Highlanders.}, journal = {American journal of human genetics}, volume = {99}, number = {3}, pages = {580-594}, pmid = {27569548}, issn = {1537-6605}, support = {K99 AA021802/AA/NIAAA NIH HHS/United States ; R00 AA021802/AA/NIAAA NIH HHS/United States ; }, mesh = {Altitude ; Animals ; Asian Continental Ancestry Group/*genetics ; China/ethnology ; Ethnic Groups/genetics ; Gene Flow/*genetics ; Gene Pool ; Genetics, Population ; Genome, Human/*genetics ; Haplotypes/genetics ; High-Throughput Nucleotide Sequencing ; Humans ; Male ; Models, Genetic ; Neanderthals/genetics ; Oceania/ethnology ; *Phylogeny ; Selection, Genetic ; Tibet ; }, abstract = {The origin of Tibetans remains one of the most contentious puzzles in history, anthropology, and genetics. Analyses of deeply sequenced (30×-60×) genomes of 38 Tibetan highlanders and 39 Han Chinese lowlanders, together with available data on archaic and modern humans, allow us to comprehensively characterize the ancestral makeup of Tibetans and uncover their origins. Non-modern human sequences compose ∼6% of the Tibetan gene pool and form unique haplotypes in some genomic regions, where Denisovan-like, Neanderthal-like, ancient-Siberian-like, and unknown ancestries are entangled and elevated. The shared ancestry of Tibetan-enriched sequences dates back to ∼62,000-38,000 years ago, predating the Last Glacial Maximum (LGM) and representing early colonization of the plateau. Nonetheless, most of the Tibetan gene pool is of modern human origin and diverged from that of Han Chinese ∼15,000 to ∼9,000 years ago, which can be largely attributed to post-LGM arrivals. Analysis of ∼200 contemporary populations showed that Tibetans share ancestry with populations from East Asia (∼82%), Central Asia and Siberia (∼11%), South Asia (∼6%), and western Eurasia and Oceania (∼1%). Our results support that Tibetans arose from a mixture of multiple ancestral gene pools but that their origins are much more complicated and ancient than previously suspected. We provide compelling evidence of the co-existence of Paleolithic and Neolithic ancestries in the Tibetan gene pool, indicating a genetic continuity between pre-historical highland-foragers and present-day Tibetans. In particular, highly differentiated sequences harbored in highlanders' genomes were most likely inherited from pre-LGM settlers of multiple ancestral origins (SUNDer) and maintained in high frequency by natural selection.}, }
@article {pmid27517578, year = {2016}, author = {Caldararo, N}, title = {Denisovans, Melanesians, Europeans, and Neandertals: The Confusion of DNA Assumptions and the Biological Species Concept.}, journal = {Journal of molecular evolution}, volume = {83}, number = {1-2}, pages = {78-87}, pmid = {27517578}, issn = {1432-1432}, mesh = {Animals ; Biological Evolution ; DNA/genetics ; DNA, Ancient/*analysis ; Fossils ; Hominidae/genetics ; Humans ; Neanderthals/*genetics ; Sequence Alignment/methods ; Sequence Analysis, DNA/methods ; }, abstract = {A number of recent articles have appeared on the Denisova fossil remains and attempts to produce DNA sequences from them. One of these recently appeared in Science by Vernot et al. (Science 352:235-239, 2016). We would like to advance an alternative interpretation of the data presented. One concerns the problem of contamination/degradation of the determined DNA sequenced. Just as the publication of the first Neandertal sequence included an interpretation that argued that Neandertals had not contributed any genes to modern humans, the Denisovan interpretation has considerable influence on ideas regarding human evolution. The new papers, however, confuse established ideas concerning the nature of species, as well as the use of terms like premodern, Archaic Homo, and Homo heidelbergensis. Examination of these problems presents a solution by means of reinterpreting the results. Given the claims for gene transfer among a number of Mid Pleistocene hominids, it may be time to reexamine the idea of anagenesis in hominid evolution.}, }
@article {pmid27486223, year = {2016}, author = {Hubbard, TD and Murray, IA and Bisson, WH and Sullivan, AP and Sebastian, A and Perry, GH and Jablonski, NG and Perdew, GH}, title = {Divergent Ah Receptor Ligand Selectivity during Hominin Evolution.}, journal = {Molecular biology and evolution}, volume = {33}, number = {10}, pages = {2648-2658}, pmid = {27486223}, issn = {1537-1719}, support = {R01 ES004869/ES/NIEHS NIH HHS/United States ; R01 ES019964/ES/NIEHS NIH HHS/United States ; }, mesh = {Animals ; Basic Helix-Loop-Helix Transcription Factors/genetics ; Benzo(a)pyrene ; Biological Evolution ; Cytochrome P-450 CYP1A1/genetics ; Cytochrome P-450 CYP1B1/genetics ; DNA/metabolism ; Evolution, Molecular ; Hominidae/*genetics ; Humans ; Ligands ; Neanderthals/*genetics ; Polycyclic Aromatic Hydrocarbons/metabolism ; Receptors, Aryl Hydrocarbon/*genetics ; }, abstract = {We have identified a fixed nonsynonymous sequence difference between humans (Val381; derived variant) and Neandertals (Ala381; ancestral variant) in the ligand-binding domain of the aryl hydrocarbon receptor (AHR) gene. In an exome sequence analysis of four Neandertal and Denisovan individuals compared with nine modern humans, there are only 90 total nucleotide sites genome-wide for which archaic hominins are fixed for the ancestral nonsynonymous variant and the modern humans are fixed for the derived variant. Of those sites, only 27, including Val381 in the AHR, also have no reported variability in the human dbSNP database, further suggesting that this highly conserved functional variant is a rare event. Functional analysis of the amino acid variant Ala381 within the AHR carried by Neandertals and nonhuman primates indicate enhanced polycyclic aromatic hydrocarbon (PAH) binding, DNA binding capacity, and AHR mediated transcriptional activity compared with the human AHR. Also relative to human AHR, the Neandertal AHR exhibited 150-1000 times greater sensitivity to induction of Cyp1a1 and Cyp1b1 expression by PAHs (e.g., benzo(a)pyrene). The resulting CYP1A1/CYP1B1 enzymes are responsible for PAH first pass metabolism, which can result in the generation of toxic intermediates and perhaps AHR-associated toxicities. In contrast, the human AHR retains the ancestral sensitivity observed in primates to nontoxic endogenous AHR ligands (e.g., indole, indoxyl sulfate). Our findings reveal that a functionally significant change in the AHR occurred uniquely in humans, relative to other primates, that would attenuate the response to many environmental pollutants, including chemicals present in smoke from fire use during cooking.}, }
@article {pmid27486199, year = {2016}, author = {Söderholm, S and Kainov, DE and Öhman, T and Denisova, OV and Schepens, B and Kulesskiy, E and Imanishi, SY and Corthals, G and Hintsanen, P and Aittokallio, T and Saelens, X and Matikainen, S and Nyman, TA}, title = {Phosphoproteomics to Characterize Host Response During Influenza A Virus Infection of Human Macrophages.}, journal = {Molecular &amp; cellular proteomics : MCP}, volume = {15}, number = {10}, pages = {3203-3219}, pmid = {27486199}, issn = {1535-9484}, mesh = {Animals ; Computational Biology/methods ; Cyclin-Dependent Kinases/metabolism ; Gene Expression Regulation ; Host-Pathogen Interactions ; Humans ; Influenza A virus/*pathogenicity ; Influenza, Human/*metabolism ; Macrophages/metabolism/*virology ; Mice ; Phosphoproteins/*analysis ; Proteomics/*methods ; Signal Transduction ; }, abstract = {Influenza A viruses cause infections in the human respiratory tract and give rise to annual seasonal outbreaks, as well as more rarely dreaded pandemics. Influenza A viruses become quickly resistant to the virus-directed antiviral treatments, which are the current main treatment options. A promising alternative approach is to target host cell factors that are exploited by influenza viruses. To this end, we characterized the phosphoproteome of influenza A virus infected primary human macrophages to elucidate the intracellular signaling pathways and critical host factors activated upon influenza infection. We identified 1675 phosphoproteins, 4004 phosphopeptides and 4146 nonredundant phosphosites. The phosphorylation of 1113 proteins (66%) was regulated upon infection, highlighting the importance of such global phosphoproteomic profiling in primary cells. Notably, 285 of the identified phosphorylation sites have not been previously described in publicly available phosphorylation databases, despite many published large-scale phosphoproteome studies using human and mouse cell lines. Systematic bioinformatics analysis of the phosphoproteome data indicated that the phosphorylation of proteins involved in the ubiquitin/proteasome pathway (such as TRIM22 and TRIM25) and antiviral responses (such as MAVS) changed in infected macrophages. Proteins known to play roles in small GTPase-, mitogen-activated protein kinase-, and cyclin-dependent kinase- signaling were also regulated by phosphorylation upon infection. In particular, the influenza infection had a major influence on the phosphorylation profiles of a large number of cyclin-dependent kinase substrates. Functional studies using cyclin-dependent kinase inhibitors showed that the cyclin-dependent kinase activity is required for efficient viral replication and for activation of the host antiviral responses. In addition, we show that cyclin-dependent kinase inhibitors protect IAV-infected mice from death. In conclusion, we provide the first comprehensive phosphoproteome characterization of influenza A virus infection in primary human macrophages, and provide evidence that cyclin-dependent kinases represent potential therapeutic targets for more effective treatment of influenza infections.}, }
@article {pmid27463216, year = {2016}, author = {Taylor, JS and Reimchen, TE}, title = {Opsin gene repertoires in northern archaic hominids.}, journal = {Genome}, volume = {59}, number = {8}, pages = {541-549}, doi = {10.1139/gen-2015-0164}, pmid = {27463216}, issn = {1480-3321}, mesh = {Animals ; Anthropology, Physical ; Base Sequence ; Evolution, Molecular ; Genome, Human ; Hominidae/*genetics ; Humans ; Methyl-CpG-Binding Protein 2/genetics ; Neanderthals/genetics ; Opsins/*genetics ; Phylogeny ; Vision, Ocular/genetics ; }, abstract = {The Neanderthals' northern distribution, hunting techniques, and orbit breadths suggest that they were more active in dim light than modern humans. We surveyed visual opsin genes from four Neanderthals and two other archaic hominids to see if they provided additional support for this hypothesis. This analysis was motivated by the observation that alleles responsible for anomalous trichromacy in humans are more common in northern latitudes, by data suggesting that these variants might enhance vision in mesopic conditions, and by the observation that dim light active species often have fewer opsin genes than diurnal relatives. We also looked for evidence of convergent amino acid substitutions in Neanderthal opsins and orthologs from crepuscular or nocturnal species. The Altai Neanderthal, the Denisovan, and the Ust'-Ishim early modern human had opsin genes that encoded proteins identical to orthologs in the human reference genome. Opsins from the Vindija Cave Neanderthals (three females) had many nonsynonymous substitutions, including several predicted to influence colour vision (e.g., stop codons). However, the functional implications of these observations were difficult to assess, given that "control" loci, where no substitutions were expected, differed from humans to the same extent. This left unresolved the test for colour vision deficiencies in Vindija Cave Neanderthals.}, }
@article {pmid27429943, year = {2016}, author = {Ko, KH}, title = {Hominin interbreeding and the evolution of human variation.}, journal = {Journal of biological research (Thessalonike, Greece)}, volume = {23}, number = {}, pages = {17}, pmid = {27429943}, issn = {1790-045X}, abstract = {Mitochondrial Eve confirms the "out of Africa" theory, but the evidence also supports interbreeding between Homo sapiens and other hominins: Neanderthals, Denisovans, and Homo heidelbergensis. This article explains how interbreeding between early H. sapiens and archaic hominins occurred. The availability of edible insects in East Asia aided the spread of the unaggressive, highly cooperative Neanderthals, who interbred with H. sapiens in Asia, resulting in a higher admixture of Neanderthal DNA in East Asian populations. Geographical variation in degree of interbreeding between H. sapiens and Neanderthals likely contributed to neurological and behavioral differences in modern humans. Similarly, people with Denisovan genetic admixture were better able to dwell in mountainous regions, allowing their genetic legacy to cross the Himalayas and persist in Southeast Asian and Oceanian H. sapiens. In the Sub-Saharan region, unaffected by Denisovan or Neanderthal interbreeding, H. sapiens interbred with H. heidelbergensis, because high humidity militated against fire-making and allowed the survival of these non-fire-making hominins.}, }
@article {pmid27423248, year = {2017}, author = {Miga, KH}, title = {Chromosome-Specific Centromere Sequences Provide an Estimate of the Ancestral Chromosome 2 Fusion Event in Hominin Genomes.}, journal = {The Journal of heredity}, volume = {108}, number = {1}, pages = {45-52}, doi = {10.1093/jhered/esw039}, pmid = {27423248}, issn = {1465-7333}, mesh = {Animals ; *Centromere ; Chromosomes, Human, Pair 2 ; *Chromosomes, Mammalian ; DNA, Satellite ; *Evolution, Molecular ; *Genome ; Genomics/methods ; Hominidae/*genetics ; Humans ; Pan troglodytes/genetics ; }, abstract = {Human chromosome 2 is a product of a telomere fusion of two ancestral chromosomes and loss/degeneration of one of the two original centromeres. Genomic signatures of this event are limited to inverted telomeric repeats at the precise site of chromosomal fusion and to the small amount of relic centromeric sequences that remain on 2q21.2. Unlike the site of fusion, which is enriched for sequences that are shared elsewhere in the human genome, the region of the nonfunctioning and degenerate ancestral centromere appears to share limited similarity with other sites in the human genome, thereby providing an opportunity to study this genomic arrangement in short, fragmented ancient DNA genomic datasets. Here, chromosome-assigned satellite DNAs are used to study shared centromere sequence organization in Denisovan and Neandertal genomes. By doing so, one is able to provide evidence for the presence of both active and degenerate centromeric satellite profiles on chromosome 2 in these archaic genomes, supporting the hypothesis that the chromosomal fusion event took place prior to our last common ancestor with Denisovan and Neandertal hominins and presenting a genomic reference for predicting karyotype in ancient genomic datasets.}, }
@article {pmid27358932, year = {2016}, author = {Bogomolov, DV and Zbrueva, YV and Putintsev, VA and Denisova, OP}, title = {[Forensic medical diagnostics of intra-vitality of the strangulation mark by morphological methods].}, journal = {Sudebno-meditsinskaia ekspertiza}, volume = {59}, number = {2}, pages = {40-43}, doi = {10.17116/sudmed201659240-43}, pmid = {27358932}, issn = {0039-4521}, mesh = {*Asphyxia/metabolism/pathology ; Forensic Pathology/methods ; Humans ; Immunohistochemistry ; }, abstract = {The objective of the present study WaS to overview the current domestic and foreign literature concerning the up-to-date methods employed for the expert evaluation of intra-vitality of the strangulation mark. The secondary objective was to propose the new approaches for addressing this problem. The methods of expert diagnostics with a view to determining the time of infliction of injuries as exemplified by mechanical asphyxia are discussed. It is concluded that immunohistochemical and morphometric studies provide the most promising tools for the evaluation of intra-vitality of the strangulation mark for the purpose of forensic medical expertise.}, }
@article {pmid27274045, year = {2016}, author = {Slatkin, M and Racimo, F}, title = {Ancient DNA and human history.}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {113}, number = {23}, pages = {6380-6387}, pmid = {27274045}, issn = {1091-6490}, support = {R01 GM040282/GM/NIGMS NIH HHS/United States ; }, mesh = {Animals ; DNA Contamination ; *DNA, Ancient ; Fossils ; Genome ; Hominidae/*genetics ; Humans ; }, abstract = {We review studies of genomic data obtained by sequencing hominin fossils with particular emphasis on the unique information that ancient DNA (aDNA) can provide about the demographic history of humans and our closest relatives. We concentrate on nuclear genomic sequences that have been published in the past few years. In many cases, particularly in the Arctic, the Americas, and Europe, aDNA has revealed historical demographic patterns in a way that could not be resolved by analyzing present-day genomes alone. Ancient DNA from archaic hominins has revealed a rich history of admixture between early modern humans, Neanderthals, and Denisovans, and has allowed us to disentangle complex selective processes. Information from aDNA studies is nowhere near saturation, and we believe that future aDNA sequences will continue to change our understanding of hominin history.}, }
@article {pmid27182564, year = {2016}, author = {Rabinovich, IM and Rabinovich, OF and Abramova, ES and Denisova, MA}, title = {[Clinical and pathogenetic aspects of various forms of cheilitis].}, journal = {Stomatologiia}, volume = {95}, number = {1}, pages = {67-72}, doi = {10.17116/stomat201695167-72}, pmid = {27182564}, issn = {0039-1735}, mesh = {Cheilitis/drug therapy/*etiology/*pathology ; Humans ; Microscopic Angioscopy/methods ; }, }
@article {pmid27176232, year = {2016}, author = {Zhao, G and Denisova, K and Sehatpour, P and Long, J and Gui, W and Qiao, J and Javitt, DC and Wang, Z}, title = {Fractal Dimension Analysis of Subcortical Gray Matter Structures in Schizophrenia.}, journal = {PloS one}, volume = {11}, number = {5}, pages = {e0155415}, pmid = {27176232}, issn = {1932-6203}, support = {T32 MH016434/MH/NIMH NIH HHS/United States ; }, abstract = {A failure of adaptive inference-misinterpreting available sensory information for appropriate perception and action-is at the heart of clinical manifestations of schizophrenia, implicating key subcortical structures in the brain including the hippocampus. We used high-resolution, three-dimensional (3D) fractal geometry analysis to study subtle and potentially biologically relevant structural alterations (in the geometry of protrusions, gyri and indentations, sulci) in subcortical gray matter (GM) in patients with schizophrenia relative to healthy individuals. In particular, we focus on utilizing Fractal Dimension (FD), a compact shape descriptor that can be computed using inputs with irregular (i.e., not necessarily smooth) surfaces in order to quantify complexity (of geometrical properties and configurations of structures across spatial scales) of subcortical GM in this disorder. Probabilistic (entropy-based) information FD was computed based on the box-counting approach for each of the seven subcortical structures, bilaterally, as well as the brainstem from high-resolution magnetic resonance (MR) images in chronic patients with schizophrenia (n = 19) and age-matched healthy controls (n = 19) (age ranges: patients, 22.7-54.3 and healthy controls, 24.9-51.6 years old). We found a significant reduction of FD in the left hippocampus (median: 2.1460, range: 2.07-2.18 vs. median: 2.1730, range: 2.15-2.23, p<0.001; Cohen's effect size, U3 = 0.8158 (95% Confidence Intervals, CIs: 0.6316, 1.0)), the right hippocampus (median: 2.1430, range: 2.05-2.19 vs. median: 2.1760, range: 2.12-2.21, p = 0.004; U3 = 0.8421 (CIs: 0.5263, 1)), as well as left thalamus (median: 2.4230, range: 2.40-2.44, p = 0.005; U3 = 0.7895 (CIs: 0.5789, 0.9473)) in schizophrenia patients, relative to healthy individuals. Our findings provide in-vivo quantitative evidence for reduced surface complexity of hippocampus, with reduced FD indicating a less complex, less regular GM surface detected in schizophrenia.}, }
@article {pmid27160892, year = {2016}, author = {Johnston, SC and Amarenco, P and Albers, GW and Denison, H and Easton, JD and Evans, SR and Held, P and Jonasson, J and Minematsu, K and Molina, CA and Wang, Y and Wong, KS and , }, title = {Ticagrelor versus Aspirin in Acute Stroke or Transient Ischemic Attack.}, journal = {The New England journal of medicine}, volume = {375}, number = {1}, pages = {35-43}, doi = {10.1056/NEJMoa1603060}, pmid = {27160892}, issn = {1533-4406}, mesh = {Adenosine/adverse effects/*analogs &amp; derivatives/therapeutic use ; Aged ; Aspirin/adverse effects/*therapeutic use ; Double-Blind Method ; Female ; Hemorrhage/chemically induced ; Humans ; Ischemic Attack, Transient/*drug therapy ; Kaplan-Meier Estimate ; Male ; Middle Aged ; Platelet Aggregation Inhibitors/adverse effects/*therapeutic use ; Purinergic P2Y Receptor Antagonists/adverse effects/therapeutic use ; Stroke/*drug therapy ; Ticagrelor ; }, abstract = {BACKGROUND: Ticagrelor may be a more effective antiplatelet therapy than aspirin for the prevention of recurrent stroke and cardiovascular events in patients with acute cerebral ischemia.<br><br>METHODS: We conducted an international double-blind, controlled trial in 674 centers in 33 countries, in which 13,199 patients with a nonsevere ischemic stroke or high-risk transient ischemic attack who had not received intravenous or intraarterial thrombolysis and were not considered to have had a cardioembolic stroke were randomly assigned within 24 hours after symptom onset, in a 1:1 ratio, to receive either ticagrelor (180 mg loading dose on day 1 followed by 90 mg twice daily for days 2 through 90) or aspirin (300 mg on day 1 followed by 100 mg daily for days 2 through 90). The primary end point was the time to the occurrence of stroke, myocardial infarction, or death within 90 days.<br><br>RESULTS: During the 90 days of treatment, a primary end-point event occurred in 442 of the 6589 patients (6.7%) treated with ticagrelor, versus 497 of the 6610 patients (7.5%) treated with aspirin (hazard ratio, 0.89; 95% confidence interval [CI], 0.78 to 1.01; P=0.07). Ischemic stroke occurred in 385 patients (5.8%) treated with ticagrelor and in 441 patients (6.7%) treated with aspirin (hazard ratio, 0.87; 95% CI, 0.76 to 1.00). Major bleeding occurred in 0.5% of patients treated with ticagrelor and in 0.6% of patients treated with aspirin, intracranial hemorrhage in 0.2% and 0.3%, respectively, and fatal bleeding in 0.1% and 0.1%.<br><br>CONCLUSIONS: In our trial involving patients with acute ischemic stroke or transient ischemic attack, ticagrelor was not found to be superior to aspirin in reducing the rate of stroke, myocardial infarction, or death at 90 days. (Funded by AstraZeneca; ClinicalTrials.gov number, NCT01994720.).}, }
@article {pmid27070257, year = {2016}, author = {Denisova, NP and Rogov, DY and Rzaev, DA and Khabarova, EA and Dmitriev, AB}, title = {[Spinal cord stimulation in the treatment of chronic pain syndromes].}, journal = {Zhurnal voprosy neirokhirurgii imeni N. N. Burdenko}, volume = {80}, number = {2}, pages = {47-52}, doi = {10.17116/neiro201680247-52}, pmid = {27070257}, issn = {0042-8817}, mesh = {Adult ; Aged ; Aged, 80 and over ; Chronic Pain/*therapy ; Electric Stimulation Therapy/*methods ; Female ; Humans ; Male ; Middle Aged ; Neuralgia/*therapy ; *Spinal Cord ; Syndrome ; }, abstract = {AIM: The study objective was to estimate the efficacy of chronic epidural spinal cord stimulation in the treatment of patients with neuropathic pain syndrome.<br><br>MATERIAL AND METHODS: A system for chronic spinal cord stimulation (St. Jude) was implanted to 75 patients with neuropathic pain syndrome. Fifty three (70.7%) patients were diagnosed with failed back surgery syndrome (FBSS); 9 (12.0%) patients had complex regional pain syndrome type II; 4 (5.3%) patients had diabetic polyneuropathy of the lower limbs; 3 (4.0%) patients had idiopathic pelvic-perineal pain; 2 (2.7%) patients had spinal stroke pain; 2 (2.7%) patients had postherpetic intercostal neuralgia; 1 (1.3%) patient had stump pain; 1 (1.3%) patient had spinal cord injury pain. The treatment efficacy was evaluated using the visual analog scale (VAS) and DN4 questionnaire. The follow-up period ranged from 6 to 18 months.<br><br>RESULTS: 136 patients underwent test stimulation at the Center in 2014. A significant reduction in pain was observed in 75 (55.1%) patients. These patients underwent the second stage of surgery that included implantation of permanent electrodes and a generator. The mean VAS score was 6.5 (maximum: 10; minimum: 5) before surgery, 3.2 at discharge, and 3.1 after 3 and 6 months. The VAS score amounted to 3.6 after 12 months. Complications in the form of pain at the generator implantation site and the need for removal of the system occurred in 2 patients (2.6%), electrode migration was observed in 4 (5.3%) cases.<br><br>CONCLUSION: Chronic epidural spinal cord stimulation is an effective and safe technique for the treatment of drug-resistant chronic neurogenic pain syndromes.}, }
@article {pmid27063929, year = {2016}, author = {Houldcroft, CJ and Underdown, SJ}, title = {Neanderthal genomics suggests a pleistocene time frame for the first epidemiologic transition.}, journal = {American journal of physical anthropology}, volume = {160}, number = {3}, pages = {379-388}, doi = {10.1002/ajpa.22985}, pmid = {27063929}, issn = {1096-8644}, mesh = {Animals ; Anthropology, Physical ; Communicable Diseases/*genetics/history/*immunology/microbiology ; Evolution, Molecular ; Fossils ; Genome/*genetics/*immunology ; Genomics ; History, Ancient ; Humans ; Neanderthals/*genetics/*immunology ; }, abstract = {High quality Altai Neanderthal and Denisovan genomes are revealing which regions of archaic hominin DNA have persisted in the modern human genome. A number of these regions are associated with response to infection and immunity, with a suggestion that derived Neanderthal alleles found in modern Europeans and East Asians may be associated with autoimmunity. As such Neanderthal genomes are an independent line of evidence of which infectious diseases Neanderthals were genetically adapted to. Sympathetically, human genome adaptive introgression is an independent line of evidence of which infectious diseases were important for AMH coming in to Eurasia and interacting with Neanderthals. The Neanderthals and Denisovans present interesting cases of hominin hunter-gatherers adapted to a Eurasian rather than African infectious disease package. Independent sources of DNA-based evidence allow a re-evaluation of the first epidemiologic transition and how infectious disease affected Pleistocene hominins. By combining skeletal, archaeological and genetic evidence from modern humans and extinct Eurasian hominins, we question whether the first epidemiologic transition in Eurasia featured a new package of infectious diseases or a change in the impact of existing pathogens. Coupled with pathogen genomics, this approach supports the view that many infectious diseases are pre-Neolithic, and the list continues to expand. The transfer of pathogens between hominin populations, including the expansion of pathogens from Africa, may also have played a role in the extinction of the Neanderthals and offers an important mechanism to understand hominin-hominin interactions well back beyond the current limits for aDNA extraction from fossils alone. Am J Phys Anthropol 160:379-388, 2016. © 2016 Wiley Periodicals, Inc.}, }
@article {pmid27032491, year = {2016}, author = {Sankararaman, S and Mallick, S and Patterson, N and Reich, D}, title = {The Combined Landscape of Denisovan and Neanderthal Ancestry in Present-Day Humans.}, journal = {Current biology : CB}, volume = {26}, number = {9}, pages = {1241-1247}, pmid = {27032491}, issn = {1879-0445}, support = {//Howard Hughes Medical Institute/United States ; K99 GM111744/GM/NIGMS NIH HHS/United States ; R00 GM111744/GM/NIGMS NIH HHS/United States ; R01 GM100233/GM/NIGMS NIH HHS/United States ; }, mesh = {Animals ; Continental Population Groups/*genetics ; Demography ; Genome, Human ; Humans ; Models, Genetic ; Neanderthals/*genetics ; Time Factors ; }, abstract = {Some present-day humans derive up to ∼5% [1] of their ancestry from archaic Denisovans, an even larger proportion than the ∼2% from Neanderthals [2]. We developed methods that can disambiguate the locations of segments of Denisovan and Neanderthal ancestry in present-day humans and applied them to 257 high-coverage genomes from 120 diverse populations, among which were 20 individual Oceanians with high Denisovan ancestry [3]. In Oceanians, the average size of Denisovan fragments is larger than Neanderthal fragments, implying a more recent average date of Denisovan admixture in the history of these populations (p = 0.00004). We document more Denisovan ancestry in South Asia than is expected based on existing models of history, reflecting a previously undocumented mixture related to archaic humans (p = 0.0013). Denisovan ancestry, just like Neanderthal ancestry, has been deleterious on a modern human genetic background, as reflected by its depletion near genes. Finally, the reduction of both archaic ancestries is especially pronounced on chromosome X and near genes more highly expressed in testes than other tissues (p = 1.2 × 10(-7) to 3.2 × 10(-7) for Denisovan and 2.2 × 10(-3) to 2.9 × 10(-3) for Neanderthal ancestry even after controlling for differences in level of selective constraint across gene classes). This suggests that reduced male fertility may be a general feature of mixtures of human populations diverged by >500,000 years.}, }
@article {pmid27020421, year = {2016}, author = {Brown, S and Higham, T and Slon, V and Pääbo, S and Meyer, M and Douka, K and Brock, F and Comeskey, D and Procopio, N and Shunkov, M and Derevianko, A and Buckley, M}, title = {Identification of a new hominin bone from Denisova Cave, Siberia using collagen fingerprinting and mitochondrial DNA analysis.}, journal = {Scientific reports}, volume = {6}, number = {}, pages = {23559}, pmid = {27020421}, issn = {2045-2322}, mesh = {Animals ; Antineoplastic Combined Chemotherapy Protocols ; Bone and Bones/*metabolism ; Collagen/analysis/*metabolism ; Cyclophosphamide ; DNA, Mitochondrial/chemistry/classification/*genetics ; Doxorubicin ; Evolution, Molecular ; *Fossils ; Hominidae ; Humans ; Phylogeny ; Podophyllotoxin ; Radiometric Dating ; Sequence Analysis, DNA ; Siberia ; Tandem Mass Spectrometry ; Time Factors ; Vincristine ; }, abstract = {DNA sequencing has revolutionised our understanding of archaic humans during the Middle and Upper Palaeolithic. Unfortunately, while many Palaeolithic sites contain large numbers of bones, the majority of these lack the diagnostic features necessary for traditional morphological identification. As a result the recovery of Pleistocene-age human remains is extremely rare. To circumvent this problem we have applied a method of collagen fingerprinting to more than 2000 fragmented bones from the site of Denisova Cave, Russia, in order to facilitate the discovery of human remains. As a result of our analysis a single hominin bone (Denisova 11) was identified, supported through in-depth peptide sequencing analysis, and found to carry mitochondrial DNA of the Neandertal type. Subsequent radiocarbon dating revealed the bone to be >50,000 years old. Here we demonstrate the huge potential collagen fingerprinting has for identifying hominin remains in highly fragmentary archaeological assemblages, improving the resources available for wider studies into human evolution.}, }
@article {pmid26995655, year = {2016}, author = {Árnason, Ú}, title = {The Out of Africa hypothesis and the ancestry of recent humans: Cherchez la femme (et l'homme).}, journal = {Gene}, volume = {585}, number = {1}, pages = {9-12}, doi = {10.1016/j.gene.2016.03.018}, pmid = {26995655}, issn = {1879-0038}, mesh = {Africa ; Animals ; Asia ; *Biological Evolution ; China ; DNA, Mitochondrial/genetics ; Europe ; *Evolution, Molecular ; Female ; *Fossils ; Gene Flow ; Humans ; Male ; Neanderthals/*genetics ; Phylogeny ; }, abstract = {The Out of Africa hypothesis (OOAH) has been a mainstay in the discussion of human evolution since its presentation in the 1980's. However, recent advances in palaeontology and molecular genetics have made it possible to examine the hypothesis in a manner that was inconceivable at the time of its proposal. The palaeontological progress relates to early Homo finds in the Caucasus, Denisova finds in the Altai Mountains and Neanderthal finds in a wide range of localities from the Altai Mountains, the Caucasus, the Levant, Asia Minor, southern and Central Europe and the Iberian Peninsula. The Eurasian location of these finds and recognition of the principle of Last common ancestor (LCA) lend no support to OOAH. The same conclusion is drawn from genomic findings, which (a) have revealed the presence of Denisovan and Neanderthal nuclear DNA, primarily in the genomes of recent Eurasians and (b) have shown genomic introgression from early modern humans into Neanderthals in the Altai Mountains. Similarly, archaeological finds in Sulawesi and the discovery of ≈100,000years old human teeth in southern China constitute strong independent challenges to OOAH. The genomic and palaeogenomic results and the new palaeontological and archaeological discoveries suggest (a) that the ancestors of modern humans had their origin in a Eurasian (largely Asian) biogeographic region which may also have extended into NE Africa, and (b) that the founders of basal African lineages became separated, geographically and genetically, in the westernmost part of this region and spread from there to different parts of the African continent.}, }
@article {pmid26989198, year = {2016}, author = {Vernot, B and Tucci, S and Kelso, J and Schraiber, JG and Wolf, AB and Gittelman, RM and Dannemann, M and Grote, S and McCoy, RC and Norton, H and Scheinfeldt, LB and Merriwether, DA and Koki, G and Friedlaender, JS and Wakefield, J and Pääbo, S and Akey, JM}, title = {Excavating Neandertal and Denisovan DNA from the genomes of Melanesian individuals.}, journal = {Science (New York, N.Y.)}, volume = {352}, number = {6282}, pages = {235-239}, pmid = {26989198}, issn = {1095-9203}, support = {R01 GM110068/GM/NIGMS NIH HHS/United States ; 5R01GM110068/GM/NIGMS NIH HHS/United States ; }, mesh = {Animals ; DNA/*genetics ; Genetic Variation ; Genome, Human/*genetics ; Humans ; Melanesia ; Neanderthals/*genetics ; Oceanic Ancestry Group/*genetics ; Sequence Analysis, DNA ; }, abstract = {Although Neandertal sequences that persist in the genomes of modern humans have been identified in Eurasians, comparable studies in people whose ancestors hybridized with both Neandertals and Denisovans are lacking. We developed an approach to identify DNA inherited from multiple archaic hominin ancestors and applied it to whole-genome sequences from 1523 geographically diverse individuals, including 35 previously unknown Island Melanesian genomes. In aggregate, we recovered 1.34 gigabases and 303 megabases of the Neandertal and Denisovan genome, respectively. We use these maps of archaic sequences to show that Neandertal admixture occurred multiple times in different non-African populations, characterize genomic regions that are significantly depleted of archaic sequences, and identify signatures of adaptive introgression.}, }
@article {pmid26978613, year = {2016}, author = {Kovalkova, NA and Denisova, DV and Polonskaya, YV and Kashtanova, EV}, title = {[The incidence of respiratory symptoms and their associations with serum cotinine levels as a marker of tobacco smoking in 25-45-year-old Novosibirsk dwellers].}, journal = {Terapevticheskii arkhiv}, volume = {88}, number = {1}, pages = {70-74}, doi = {10.17116/terarkh201688170-74}, pmid = {26978613}, issn = {0040-3660}, mesh = {Adult ; Cotinine/*blood ; *Cough/epidemiology/etiology ; Female ; Humans ; Incidence ; Male ; *Respiratory Sounds/etiology/physiopathology ; Respiratory System/drug effects/physiopathology ; Siberia/epidemiology ; *Smoking/adverse effects/blood/epidemiology/physiopathology ; Time Factors ; }, abstract = {AIM: To study the incidence of respiratory symptoms and to reveal their associations with serum cotinine levels (SCL) in 25-45-year-old Novosibirsk dwellers.<br><br>SUBJECTS AND METHODS: The WHO respiratory symptom questionnaire and the ECRHS screening questionnaire were used for a population-based survey conducted in Novosibirsk to identify respiratory symptoms; 545 people replied to the questions available in the questionnaires. SCL was determined by enzyme immunoassay on a random subsample of 182 examinees.<br><br>RESULTS: The incidence of respiratory symptoms was determined among the 25-45-year-old Novosibirsk dwellers: cough (27.7%), more than 3-month cough per year (22%), sputum discharge (25%), forced respiration/wheezing in the past year (22.6%), suffocation fits in the past year (5.3%), and cough/forced respiration/stertor bouts by breathing cold air (14.9%) or contacting animals, plants, or chemical agents (16.5%). There was a significant positive correlation between SCL and the presence of cough, more than 3-month cough per year, sputum discharge, forced respiration/wheezing in the past year (compared to the examinees who did not report these symptoms). The median SCL proved to be significantly higher in the people who complained of cough, more than 3-month cough per year, sputum discharge, and forced respiration/wheezing in the past year (compared to the examinees who did not report these symptoms). The people who had a SCL of more than 3 ng/ml were ascertained to be at higher risk of cough, more than 3-month cough per year, sputum discharge, and forced respiration/wheezing in the past year than those who had a SCL of less than 3 ng/ml.<br><br>CONCLUSION: The incidence of respiratory symptoms was determined among the 25-45-year-old Novosibirsk dwellers; SCL was found to be associated with the symptoms characteristic of bronchial obstructive diseases; the expediency of using the SCL threshold of 3 ng/ml as a marker of tobacco smoking was confirmed.}, }
@article {pmid26976447, year = {2016}, author = {Meyer, M and Arsuaga, JL and de Filippo, C and Nagel, S and Aximu-Petri, A and Nickel, B and Martínez, I and Gracia, A and Bermúdez de Castro, JM and Carbonell, E and Viola, B and Kelso, J and Prüfer, K and Pääbo, S}, title = {Nuclear DNA sequences from the Middle Pleistocene Sima de los Huesos hominins.}, journal = {Nature}, volume = {531}, number = {7595}, pages = {504-507}, pmid = {26976447}, issn = {1476-4687}, mesh = {Alleles ; Animals ; DNA, Mitochondrial/genetics ; Fossils ; Genome, Mitochondrial/genetics ; Hominidae/classification/*genetics ; Male ; Neanderthals/classification/genetics ; *Phylogeny ; Sequence Alignment ; Spain ; }, abstract = {A unique assemblage of 28 hominin individuals, found in Sima de los Huesos in the Sierra de Atapuerca in Spain, has recently been dated to approximately 430,000 years ago. An interesting question is how these Middle Pleistocene hominins were related to those who lived in the Late Pleistocene epoch, in particular to Neanderthals in western Eurasia and to Denisovans, a sister group of Neanderthals so far known only from southern Siberia. While the Sima de los Huesos hominins share some derived morphological features with Neanderthals, the mitochondrial genome retrieved from one individual from Sima de los Huesos is more closely related to the mitochondrial DNA of Denisovans than to that of Neanderthals. However, since the mitochondrial DNA does not reveal the full picture of relationships among populations, we have investigated DNA preservation in several individuals found at Sima de los Huesos. Here we recover nuclear DNA sequences from two specimens, which show that the Sima de los Huesos hominins were related to Neanderthals rather than to Denisovans, indicating that the population divergence between Neanderthals and Denisovans predates 430,000 years ago. A mitochondrial DNA recovered from one of the specimens shares the previously described relationship to Denisovan mitochondrial DNAs, suggesting, among other possibilities, that the mitochondrial DNA gene pool of Neanderthals turned over later in their history.}, }
@article {pmid26966016, year = {2016}, author = {Ghirotto, S and Tassi, F and Barbujani, G and Pattini, L and Hayward, C and Vollenweider, P and Bochud, M and Rampoldi, L and Devuyst, O}, title = {The Uromodulin Gene Locus Shows Evidence of Pathogen Adaptation through Human Evolution.}, journal = {Journal of the American Society of Nephrology : JASN}, volume = {27}, number = {10}, pages = {2983-2996}, pmid = {26966016}, issn = {1533-3450}, support = {295733//European Research Council/International ; GGP14263//Telethon/Italy ; MC_PC_U127561128//Medical Research Council/United Kingdom ; }, mesh = {Animals ; *Evolution, Molecular ; Genetic Loci ; Genetic Markers ; Genetic Variation ; Humans ; Urinary Tract Infections/genetics ; Uromodulin/*genetics ; }, abstract = {Common variants in the UMOD gene encoding uromodulin, associated with risk of hypertension and CKD in the general population, increase UMOD expression and urinary excretion of uromodulin, causing salt-sensitive hypertension and renal lesions. To determine the effect of selective pressure on variant frequency, we investigated the allelic frequency of the lead UMOD variant rs4293393 in 156 human populations, in eight ancient human genomes, and in primate genomes. The T allele of rs4293393, associated with CKD risk, has high frequency in most modern populations and was the one detected in primate genomes. In contrast, we identified only the derived, C allele in Denisovan and Neanderthal genomes. The distribution of the UMOD ancestral allele did not follow the ancestral susceptibility model observed for variants associated with salt-sensitive hypertension. Instead, the global frequencies of the UMOD alleles significantly correlated with pathogen diversity (bacteria, helminths) and prevalence of antibiotic-resistant urinary tract infections (UTIs). The inverse correlation found between urinary levels of uromodulin and markers of UTIs in the general population substantiates the link between UMOD variants and protection against UTIs. These data strongly suggest that the UMOD ancestral allele, driving higher urinary excretion of uromodulin, has been kept at a high frequency because of its protective effect against UTIs.}, }
@article {pmid26911356, year = {2016}, author = {Malyarchuk, BA and Derenko, M and Denisova, G and Woźniak, M and Rogalla, U and Dambueva, I and Grzybowski, T}, title = {Y chromosome haplotype diversity in Mongolic-speaking populations and gene conversion at the duplicated STR DYS385a,b in haplogroup C3-M407.}, journal = {Journal of human genetics}, volume = {61}, number = {6}, pages = {491-496}, pmid = {26911356}, issn = {1435-232X}, mesh = {Alleles ; Asian Continental Ancestry Group/*genetics ; China ; *Chromosomes, Human, Y ; Cluster Analysis ; Ethnic Groups/genetics ; Evolution, Molecular ; *Gene Conversion ; Gene Frequency ; Genetic Loci ; *Genetic Variation ; *Genetics, Population ; *Haplotypes ; Humans ; Male ; *Microsatellite Repeats ; Mutation ; Russia ; }, abstract = {Y chromosome microsatellite (Y-STR) diversity has been studied in different Mongolic-speaking populations from South Siberia, Mongolia, North-East China and East Europe. The results obtained indicate that the Mongolic-speaking populations clustered into two groups, with one group including populations from eastern part of South Siberia and Central Asia (the Buryats, Barghuts and Khamnigans) and the other group including populations from western part of Central Asia and East Europe (the Mongols and Kalmyks). High frequency of haplogroup C3-M407 (>50%) is present in the Buryats, Barghuts and Khamnigans, whereas in the Mongols and Kalmyks its frequency is much lower. In addition, two allelic combinations in DYS385a,b loci of C3-M407 haplotypes have been observed: the combination 11,18 (as well as 11,17 and 11,19) is frequent in different Mongolic-speaking populations, but the 11,11 branch is present mainly in the Kalmyks and Mongols. Results of locus-specific sequencing suggest that the action of gene conversion is a more likely explanation for origin of homoallelic 11,11 combination. Moreover, analysis of median networks of Y-STR haplotypes demonstrates that at least two gene conversion events can be revealed-one of them has probably occurred among the Mongols, and the other event occurred in the Barghuts. These two events give an average gene conversion rate range of 0.24-7.1 × 10(-3) per generation.}, }
@article {pmid26898827, year = {2016}, author = {Gokhman, D and Meshorer, E and Carmel, L}, title = {Epigenetics: It's Getting Old. Past Meets Future in Paleoepigenetics.}, journal = {Trends in ecology &amp; evolution}, volume = {31}, number = {4}, pages = {290-300}, doi = {10.1016/j.tree.2016.01.010}, pmid = {26898827}, issn = {1872-8383}, mesh = {Animals ; DNA/genetics ; DNA Methylation ; *Epigenesis, Genetic ; Hominidae/*genetics ; }, abstract = {Recent years have witnessed the rise of ancient DNA (aDNA) technology, allowing comparative genomics to be carried out at unprecedented time resolution. While it is relatively straightforward to use aDNA to identify recent genomic changes, it is much less clear how to utilize it to study changes in epigenetic regulation. Here we review recent works demonstrating that highly degraded aDNA still contains sufficient information to allow reconstruction of epigenetic signals, including DNA methylation and nucleosome positioning maps. We discuss challenges arising from the tissue specificity of epigenetics, and show how some of them might in fact turn into advantages. Finally, we introduce a method to infer methylation states in tissues that do not tend to be preserved over time.}, }
@article {pmid26891221, year = {2016}, author = {Balistreri, G and Viiliäinen, J and Turunen, M and Diaz, R and Lyly, L and Pekkonen, P and Rantala, J and Ojala, K and Sarek, G and Teesalu, M and Denisova, O and Peltonen, K and Julkunen, I and Varjosalo, M and Kainov, D and Kallioniemi, O and Laiho, M and Taipale, J and Hautaniemi, S and Ojala, PM}, title = {Oncogenic Herpesvirus Utilizes Stress-Induced Cell Cycle Checkpoints for Efficient Lytic Replication.}, journal = {PLoS pathogens}, volume = {12}, number = {2}, pages = {e1005424}, pmid = {26891221}, issn = {1553-7374}, mesh = {Cell Cycle Checkpoints/*genetics ; Cell Line, Tumor ; DNA Replication ; Gene Expression Regulation, Viral/*genetics ; Herpesvirus 8, Human/*genetics ; Humans ; RNA, Small Interfering/genetics ; Sarcoma, Kaposi/metabolism/virology ; Stress, Physiological/*genetics ; Virus Activation/physiology ; Virus Latency/genetics ; *Virus Replication/genetics ; }, abstract = {Kaposi's sarcoma herpesvirus (KSHV) causes Kaposi's sarcoma and certain lymphoproliferative malignancies. Latent infection is established in the majority of tumor cells, whereas lytic replication is reactivated in a small fraction of cells, which is important for both virus spread and disease progression. A siRNA screen for novel regulators of KSHV reactivation identified the E3 ubiquitin ligase MDM2 as a negative regulator of viral reactivation. Depletion of MDM2, a repressor of p53, favored efficient activation of the viral lytic transcription program and viral reactivation. During lytic replication cells activated a p53 response, accumulated DNA damage and arrested at G2-phase. Depletion of p21, a p53 target gene, restored cell cycle progression and thereby impaired the virus reactivation cascade delaying the onset of virus replication induced cytopathic effect. Herpesviruses are known to reactivate in response to different kinds of stress, and our study now highlights the molecular events in the stressed host cell that KSHV has evolved to utilize to ensure efficient viral lytic replication.}, }
@article {pmid26886800, year = {2016}, author = {Kuhlwilm, M and Gronau, I and Hubisz, MJ and de Filippo, C and Prado-Martinez, J and Kircher, M and Fu, Q and Burbano, HA and Lalueza-Fox, C and de la Rasilla, M and Rosas, A and Rudan, P and Brajkovic, D and Kucan, Ž and Gušic, I and Marques-Bonet, T and Andrés, AM and Viola, B and Pääbo, S and Meyer, M and Siepel, A and Castellano, S}, title = {Ancient gene flow from early modern humans into Eastern Neanderthals.}, journal = {Nature}, volume = {530}, number = {7591}, pages = {429-433}, pmid = {26886800}, issn = {1476-4687}, support = {R01 GM102192/GM/NIGMS NIH HHS/United States ; GM102192/GM/NIGMS NIH HHS/United States ; U01 MH106874/MH/NIMH NIH HHS/United States ; }, mesh = {Altitude ; Animals ; Bayes Theorem ; Chromosomes, Human, Pair 21/genetics ; Croatia/ethnology ; Gene Flow/*genetics ; Genome, Human/genetics ; Genomics ; Haplotypes/genetics ; Heterozygote ; Humans ; Hybridization, Genetic/genetics ; Neanderthals/*genetics ; Phylogeny ; Population Density ; Siberia ; Spain/ethnology ; Time Factors ; }, abstract = {It has been shown that Neanderthals contributed genetically to modern humans outside Africa 47,000-65,000 years ago. Here we analyse the genomes of a Neanderthal and a Denisovan from the Altai Mountains in Siberia together with the sequences of chromosome 21 of two Neanderthals from Spain and Croatia. We find that a population that diverged early from other modern humans in Africa contributed genetically to the ancestors of Neanderthals from the Altai Mountains roughly 100,000 years ago. By contrast, we do not detect such a genetic contribution in the Denisovan or the two European Neanderthals. We conclude that in addition to later interbreeding events, the ancestors of Neanderthals from the Altai Mountains and early modern humans met and interbred, possibly in the Near East, many thousands of years earlier than previously thought.}, }
@article {pmid26883865, year = {2016}, author = {Hackinger, S and Kraaijenbrink, T and Xue, Y and Mezzavilla, M and Asan,  and van Driem, G and Jobling, MA and de Knijff, P and Tyler-Smith, C and Ayub, Q}, title = {Wide distribution and altitude correlation of an archaic high-altitude-adaptive EPAS1 haplotype in the Himalayas.}, journal = {Human genetics}, volume = {135}, number = {4}, pages = {393-402}, pmid = {26883865}, issn = {1432-1203}, support = {087576//Wellcome Trust/United Kingdom ; 098051//Wellcome Trust/United Kingdom ; }, mesh = {*Altitude ; Basic Helix-Loop-Helix Transcription Factors/*genetics ; *Haplotypes ; Humans ; Polymorphism, Single Nucleotide ; }, abstract = {High-altitude adaptation in Tibetans is influenced by introgression of a 32.7-kb haplotype from the Denisovans, an extinct branch of archaic humans, lying within the endothelial PAS domain protein 1 (EPAS1), and has also been reported in Sherpa. We genotyped 19 variants in this genomic region in 1507 Eurasian individuals, including 1188 from Bhutan and Nepal residing at altitudes between 86 and 4550 m above sea level. Derived alleles for five SNPs characterizing the core Denisovan haplotype (AGGAA) were present at high frequency not only in Tibetans and Sherpa, but also among many populations from the Himalayas, showing a significant correlation with altitude (Spearman's correlation coefficient = 0.75, p value 3.9 × 10(-11)). Seven East- and South-Asian 1000 Genomes Project individuals shared the Denisovan haplotype extending beyond the 32-kb region, enabling us to refine the haplotype structure and identify a candidate regulatory variant (rs370299814) that might be interacting in an additive manner with the derived G allele of rs150877473, the variant previously associated with high-altitude adaptation in Tibetans. Denisovan-derived alleles were also observed at frequencies of 3-14% in the 1000 Genomes Project African samples. The closest African haplotype is, however, separated from the Asian high-altitude haplotype by 22 mutations whereas only three mutations, including rs150877473, separate the Asians from the Denisovan, consistent with distant shared ancestry for African and Asian haplotypes and Denisovan adaptive introgression.}, }
@article {pmid26856178, year = {2015}, author = {Denisov, AP and Semenova, NV and Kun, OA and Denisova, OA}, title = {[COMPREHENSIVE ASSESSMENT OF HEALTH IN BABIES OF EARLY PRESCHOOL AGE].}, journal = {Gigiena i sanitariia}, volume = {94}, number = {8}, pages = {69-72}, pmid = {26856178}, issn = {0016-9900}, mesh = {Adolescent ; Adult ; Child ; *Child Health ; Child, Preschool ; Developmental Disabilities/*epidemiology/etiology ; Female ; *Health Status ; Humans ; Male ; Morbidity/trends ; Siberia/epidemiology ; Socioeconomic Factors ; Young Adult ; }, abstract = {Health of the children's population is one of the most important components of safety of the country. The incidence level in children of early age reflects an interaction of economic, ecological, social and hygienic and medico-organizational factors in society. The issue of the paper is the comprehensive assessment of health of children of the first three years of life upon indices of the morbidity rate, physical development, interrelation of given indices with the structure of the family and their social status. Indices of the physical development of boys in the all age groups exceeded the corresponding indices in girls (p < 0.05). There was also statistically significant and augmentation of indices of body weights of children along with the age (p < 0.05). The highest morbidity rate in children was established in the first year of life, the minimal one--in the third year. In the all age groups diseases of respiratory organs prevailed, at this their proportion in the total amount of diseases in the third year of life considerably exceeded the same in first and second years of life. The highest incidences of children took place in the families formed by juvenile and lonely women. Diseases of digestive organs in the second and third years of life in children from juvenile and lonely mothers were considerably enlarged on frequency (by 1,4-1,7 times), infectious and parasitic diseases (by 1,1-1,7 times) in comparison with children from full families. In the all studied types offamilies and age groups the state of health of children was worse, than in full families. There was substantiated the development of the multilevel system for the prophylaxis of losses of health in children at early preschool age.}, }
@article {pmid26845858, year = {2015}, author = {Malyarchuk, BA and Derenko, MV and Denisova, GA}, title = {[Mitochondrial Genome Variability in the Wolverine (Gulo gulo)].}, journal = {Genetika}, volume = {51}, number = {11}, pages = {1291-1296}, pmid = {26845858}, issn = {0016-6758}, mesh = {Animals ; *Evolution, Molecular ; *Genome, Mitochondrial ; Mustelidae/*genetics ; *Phylogeny ; *Polymorphism, Genetic ; }, abstract = {The nucleotide sequence of an extended mitochondrial genome segment (11473 base pairs in size) was determined in the wolverine (Gulo gulo) from Magadan oblast. Phylogenetic and statistical analyses of mitochondrial DNA (mtDNA) sequences of mustelids showed that the separation of the Gulo phylogenetic branch occurred at the Miocene--early Pliocene (about 5.6 million years ago (MYA)), while the formation of the species G. gulo took place in the Middle Pleistocene (181 and 234 thousand years ago (KYA), according to the results of molecular dating based on the variability of the extended mtDNA segment and the mitochondrial cytochrome b gene, respectively). The molecular data were in agreement with the fossil records for wolverines.}, }
@article {pmid31265485, year = {2016}, author = {Dannemann, M and Andrés, AM and Kelso, J}, title = {Erratum: Introgression of Neandertal- and Denisovan-like Haplotypes Contributes to Adaptive Variation in Human Toll-like Receptors.}, journal = {American journal of human genetics}, volume = {98}, number = {2}, pages = {399}, doi = {10.1016/j.ajhg.2016.01.012}, pmid = {31265485}, issn = {1537-6605}, abstract = {[This corrects the article DOI: 10.1016/j.ajhg.2015.11.015.].}, }
@article {pmid26748514, year = {2016}, author = {Dannemann, M and Andrés, AM and Kelso, J}, title = {Introgression of Neandertal- and Denisovan-like Haplotypes Contributes to Adaptive Variation in Human Toll-like Receptors.}, journal = {American journal of human genetics}, volume = {98}, number = {1}, pages = {22-33}, pmid = {26748514}, issn = {1537-6605}, mesh = {Adaptation, Physiological/*genetics ; Animals ; Cell Line ; *Haplotypes ; Humans ; Neanderthals/*genetics ; Polymorphism, Single Nucleotide ; Toll-Like Receptors/*genetics ; }, abstract = {Pathogens and the diseases they cause have been among the most important selective forces experienced by humans during their evolutionary history. Although adaptive alleles generally arise by mutation, introgression can also be a valuable source of beneficial alleles. Archaic humans, who lived in Europe and Western Asia for more than 200,000 years, were probably well adapted to this environment and its local pathogens. It is therefore conceivable that modern humans entering Europe and Western Asia who admixed with them obtained a substantial immune advantage from the introgression of archaic alleles. Here we document a cluster of three Toll-like receptors (TLR6-TLR1-TLR10) in modern humans that carries three distinct archaic haplotypes, indicating repeated introgression from archaic humans. Two of these haplotypes are most similar to the Neandertal genome, and the third haplotype is most similar to the Denisovan genome. The Toll-like receptors are key components of innate immunity and provide an important first line of immune defense against bacteria, fungi, and parasites. The unusually high allele frequencies and unexpected levels of population differentiation indicate that there has been local positive selection on multiple haplotypes at this locus. We show that the introgressed alleles have clear functional effects in modern humans; archaic-like alleles underlie differences in the expression of the TLR genes and are associated with increased [corrected] microbial resistance and increased allergic disease in large cohorts. This provides strong evidence for recurrent adaptive introgression at the TLR6-TLR1-TLR10 locus, resulting in differences in disease phenotypes in modern humans.}, }
@article {pmid26719974, year = {2015}, author = {Arciero, E and Biagini, SA and Chen, Y and Xue, Y and Luiselli, D and Tyler-Smith, C and Pagani, L and Ayub, Q}, title = {Genes Regulated by Vitamin D in Bone Cells Are Positively Selected in East Asians.}, journal = {PloS one}, volume = {10}, number = {12}, pages = {e0146072}, pmid = {26719974}, issn = {1932-6203}, support = {/WT_/Wellcome Trust/United Kingdom ; 098051/WT_/Wellcome Trust/United Kingdom ; }, mesh = {Asian Continental Ancestry Group/*genetics ; Bone and Bones/*metabolism ; Core Binding Factor Alpha 1 Subunit/genetics ; DNA-Binding Proteins/genetics ; Gene Frequency/genetics ; Haplotypes/genetics ; Humans ; Low Density Lipoprotein Receptor-Related Protein-5/genetics ; Osteoporosis/genetics ; Polymorphism, Single Nucleotide/genetics ; Selection, Genetic/genetics ; Trans-Activators ; Vitamin D/*genetics ; }, abstract = {Vitamin D and folate are activated and degraded by sunlight, respectively, and the physiological processes they control are likely to have been targets of selection as humans expanded from Africa into Eurasia. We investigated signals of positive selection in gene sets involved in the metabolism, regulation and action of these two vitamins in worldwide populations sequenced by Phase I of the 1000 Genomes Project. Comparing allele frequency-spectrum-based summary statistics between these gene sets and matched control genes, we observed a selection signal specific to East Asians for a gene set associated with vitamin D action in bones. The selection signal was mainly driven by three genes CXXC finger protein 1 (CXXC1), low density lipoprotein receptor-related protein 5 (LRP5) and runt-related transcription factor 2 (RUNX2). Examination of population differentiation and haplotypes allowed us to identify several candidate causal regulatory variants in each gene. Four of these candidate variants (one each in CXXC1 and RUNX2 and two in LRP5) had a >70% derived allele frequency in East Asians, but were present at lower (20-60%) frequency in Europeans as well, suggesting that the adaptation might have been part of a common response to climatic and dietary changes as humans expanded out of Africa, with implications for their role in vitamin D-dependent bone mineralization and osteoporosis insurgence. We also observed haplotype sharing between East Asians, Finns and an extinct archaic human (Denisovan) sample at the CXXC1 locus, which is best explained by incomplete lineage sorting.}, }
@article {pmid29889396, year = {2016}, author = {Reshetnikov, OV and Kurilovich, SA and Denisova, DV and Krotov, SA}, title = {PREVALENCE AND RISK FACTORS OF GERD IN ADOLESCENTS IN NOVOSIBIRSK: TEN-YEAR TRENDS.}, journal = {Eksperimental'naia i klinicheskaia gastroenterologiia = Experimental &amp; clinical gastroenterology}, volume = {}, number = {9}, pages = {54-57}, pmid = {29889396}, issn = {1682-8658}, mesh = {Adolescent ; Antibodies, Bacterial/blood ; Female ; Gastroesophageal Reflux/blood/*epidemiology ; Helicobacter Infections/blood/*epidemiology ; *Helicobacter pylori ; Humans ; Immunoglobulin G/blood ; Male ; Prevalence ; Risk Factors ; Siberia/epidemiology ; }, abstract = {The aim of the study was the study of ten-year trends (1999-2009) of GERD symptoms and risk factors in schoolchildren in Novosibirsk from 14 to 17 years.<br><br>MATERIALS AND METHODS: The comparison of the results of the screenings performed at 1999 and 2009 was analyzed. Anthropometry, the survey to identify the symptoms of GERD and eating habits, smoking and alcohol consumption, determination of lgG and CagA antibodies to Helicobacterpylori were included at the program of screenings.<br><br>RESULTS: No significant dynamics of GER symptoms in adolescents, except weekly heartburn among boys. Among the factors associated with dyspepsia and GER, there have been some mixed changes: the frequency of smoking significantly reduced, but the proportion adolescents with excess body weight have increased. Violations of the regime and diet, and Helicobocrerpylori-infection has not changed significantly.<br><br>CONCLUSION: The frequency of GERD in adolescents remains high but does not reach the prevalence of GERD in adults.}, }
@article {pmid29787668, year = {2016}, author = {Bugaeva, LI and Tyurenkov, IN and Denisova, TD and Morozova, YA}, title = {[INFLUENCE OF A NEW SUBSTANCE WITH ANTIDEPRESSANT ACTIVITY ON ORGANO- AND FETOGENESIS PROCESSES REGISTERED DURING ANTHENATAL PERIOD.].}, journal = {Eksperimental'naia i klinicheskaia farmakologiia}, volume = {79}, number = {9}, pages = {25-28}, pmid = {29787668}, issn = {0869-2092}, mesh = {Animals ; Antidepressive Agents/*pharmacology ; Female ; Fetal Development/*drug effects ; Organogenesis/*drug effects ; Pregnancy ; Rats ; }, abstract = {Experiments on pregnant female rats showed that the beta-phenylglutaminic acid hydrochloride derivative neuroglutam (glutaron), exhibiting antidepressant and anxiolytic activity upon intragastric administration in doses of 26, 130 and 650 mg/kg to female rats from 6 to 16 days of pregnancy, does not impair organo- and fetogenesis processes (developments of fetus) registered during the anthenatal period, decreases fetal death, and activates the processes of prenatal development of the fetus bv 11.1% (p < 0.001), 8.3% (p < 0.001), and 2.8% (p < 0.05), respectively.}, }
@article {pmid26693966, year = {2015}, author = {Gunbin, KV and Afonnikov, DA and Kolchanov, NA and Derevianko, AP and Rogaev, EI}, title = {The evolution of Homo sapiens denisova and Homo sapiens neanderthalensis miRNA targeting genes in the prenatal and postnatal brain.}, journal = {BMC genomics}, volume = {16 Suppl 13}, number = {}, pages = {S4}, pmid = {26693966}, issn = {1471-2164}, mesh = {Animals ; Brain/*embryology/growth &amp; development/metabolism ; *Evolution, Molecular ; *Gene Expression Regulation ; Hominidae/*genetics ; Humans ; Infant, Newborn ; MicroRNAs/*genetics ; Pan troglodytes ; RNA, Messenger/*genetics ; }, abstract = {BACKGROUND: As the evolution of miRNA genes has been found to be one of the important factors in formation of the modern type of man, we performed a comparative analysis of the evolution of miRNA genes in two archaic hominines, Homo sapiens neanderthalensis and Homo sapiens denisova, and elucidated the expression of their target mRNAs in bain.<br><br>RESULTS: A comparative analysis of the genomes of primates, including species in the genus Homo, identified a group of miRNA genes having fixed substitutions with important implications for the evolution of Homo sapiens neanderthalensis and Homo sapiens denisova. The mRNAs targeted by miRNAs with mutations specific for Homo sapiens denisova exhibited enhanced expression during postnatal brain development in modern humans. By contrast, the expression of mRNAs targeted by miRNAs bearing variations specific for Homo sapiens neanderthalensis was shown to be enhanced in prenatal brain development.<br><br>CONCLUSIONS: Our results highlight the importance of changes in miRNA gene sequences in the course of Homo sapiens denisova and Homo sapiens neanderthalensis evolution. The genetic alterations of miRNAs regulating the spatiotemporal expression of multiple genes in the prenatal and postnatal brain may contribute to the progressive evolution of brain function, which is consistent with the observations of fine technical and typological properties of tools and decorative items reported from archaeological Denisovan sites. The data also suggest that differential spatial-temporal regulation of gene products promoted by the subspecies-specific mutations in the miRNA genes might have occurred in the brains of Homo sapiens denisova and Homo sapiens neanderthalensis, potentially contributing to the cultural differences between these two archaic hominines.}, }
@article {pmid26673402, year = {2015}, author = {Hammill, JA and VanSeggelen, H and Helsen, CW and Denisova, GF and Evelegh, C and Tantalo, DG and Bassett, JD and Bramson, JL}, title = {Designed ankyrin repeat proteins are effective targeting elements for chimeric antigen receptors.}, journal = {Journal for immunotherapy of cancer}, volume = {3}, number = {}, pages = {55}, pmid = {26673402}, issn = {2051-1426}, abstract = {BACKGROUND: Adoptive cell transfer of tumor-specific T lymphocytes (T cells) is proving to be an effective strategy for treating established tumors in cancer patients. One method of generating these cells is accomplished through engineering bulk T cell populations to express chimeric antigen receptors (CARs), which are specific for tumor antigens. Traditionally, these CARs are targeted against tumor antigens using single-chain antibodies (scFv). Here we describe the use of a designed ankyrin repeat protein (DARPin) as the tumor-antigen targeting domain.<br><br>METHODS: We prepared second generation anti-HER2 CARs that were targeted to the tumor antigen by either a DARPin or scFv. The CARs were engineered into human and murine T cells. We then compared the ability of CARs to trigger cytokine production, degranulation and cytotoxicity.<br><br>RESULTS: The DARPin CARs displayed reduced surface expression relative to scFv CARs in murine cells but both CARs were expressed equally well on human T cells, suggesting that there may be a processing issue with the murine variants. In both the murine and human systems, the DARPin CARs were found to be highly functional, triggering cytokine and cytotoxic responses that were similar to those triggered by the scFv CARs.<br><br>CONCLUSIONS: These findings demonstrate the utility of DARPins as CAR-targeting agents and open up an avenue for the generation of CARs with novel antigen binding attributes.}, }
@article {pmid26664599, year = {2015}, author = {Denisova, YI and Gringolts, ML and Peregudov, AS and Krentsel, LB and Litmanovich, EA and Litmanovich, AD and Finkelshtein, ESh and Kudryavtsev, YV}, title = {Cross-metathesis of polynorbornene with polyoctenamer: a kinetic study.}, journal = {Beilstein journal of organic chemistry}, volume = {11}, number = {}, pages = {1796-1808}, pmid = {26664599}, issn = {1860-5397}, abstract = {The cross-metathesis of polynorbornene and polyoctenamer in d-chloroform mediated by the 1(st) generation Grubbs' catalyst Cl2(PCy3)2Ru=CHPh is studied by monitoring the kinetics of carbene transformation and evolution of the dyad composition of polymer chains with in situ (1)H and ex situ (13)C NMR spectroscopy. The results are interpreted in terms of a simple kinetic two-stage model. At the first stage of the reaction all Ru-benzylidene carbenes are transformed into Ru-polyoctenamers within an hour, while the polymer molar mass is considerably decreased. The second stage actually including interpolymeric reactions proceeds much slower and takes one day or more to achieve a random copolymer of norbornene and cyclooctene. Its rate is limited by the interaction of polyoctenamer-bound carbenes with polynorbornene units, which is hampered, presumably due to steric reasons. Polynorbornene-bound carbenes are detected in very low concentrations throughout the whole process thus indicating their higher reactivity, as compared with the polyoctenamer-bound ones. Macroscopic homogeneity of the reacting media is proved by dynamic light scattering from solutions containing the polymer mixture and its components. In general, the studied process can be considered as a new way to unsaturated multiblock statistical copolymers. Their structure can be controlled by the amount of catalyst, mixture composition, and reaction time. It is remarkable that this goal can be achieved with a catalyst that is not suitable for ring-opening metathesis copolymerization of norbornene and cis-cyclooctene because of their substantially different monomer reactivities.}, }
@article {pmid26634840, year = {2016}, author = {Denisova, MN and Makarova, EI and Pavlov, IN and Budaeva, VV and Sakovich, GV}, title = {Enzymatic Hydrolysis of Hydrotropic Pulps at Different Substrate Loadings.}, journal = {Applied biochemistry and biotechnology}, volume = {178}, number = {6}, pages = {1196-1206}, doi = {10.1007/s12010-015-1938-y}, pmid = {26634840}, issn = {1559-0291}, mesh = {Cellulase/*chemistry ; Cellulose/chemistry ; Hydrolysis ; Microscopy, Electron, Scanning ; Substrate Specificity ; }, abstract = {Enzymatic hydrolysis of cellulosic raw materials to produce nutrient broths for microbiological synthesis of ethanol and other valuable products is an important field of modern biotechnology. Biotechnological processing implies the selection of an effective pretreatment technique for raw materials. In this study, the hydrotropic treatment increased the reactivity of the obtained substrates toward enzymatic hydrolysis by 7.1 times for Miscanthus and by 7.3 times for oat hulls. The hydrotropic pulp from oat hulls was more reactive toward enzymatic hydrolysis compared to that from Miscanthus, despite that the substrates had similar compositions. As the initial substrate loadings were raised during enzymatic hydrolysis of the hydrotropic Miscanthus and oat hull pulps, the concentration of reducing sugars increased by 34 g/dm(3) and the yield of reducing sugars decreased by 31 %. The findings allow us to predict the efficiency of enzymatic hydrolysis of hydrotropic pulps from Miscanthus and oat hulls when scaling up the process by volume.}, }
@article {pmid26630009, year = {2015}, author = {Sawyer, S and Renaud, G and Viola, B and Hublin, JJ and Gansauge, MT and Shunkov, MV and Derevianko, AP and Prüfer, K and Kelso, J and Pääbo, S}, title = {Nuclear and mitochondrial DNA sequences from two Denisovan individuals.}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {112}, number = {51}, pages = {15696-15700}, pmid = {26630009}, issn = {1091-6490}, mesh = {Animals ; Base Sequence ; Cell Nucleus/*genetics ; DNA, Mitochondrial/*chemistry ; Evolution, Molecular ; Molecular Sequence Data ; Neanderthals/*genetics ; Phylogeny ; Sequence Analysis, DNA ; }, abstract = {Denisovans, a sister group of Neandertals, have been described on the basis of a nuclear genome sequence from a finger phalanx (Denisova 3) found in Denisova Cave in the Altai Mountains. The only other Denisovan specimen described to date is a molar (Denisova 4) found at the same site. This tooth carries a mtDNA sequence similar to that of Denisova 3. Here we present nuclear DNA sequences from Denisova 4 and a morphological description, as well as mitochondrial and nuclear DNA sequence data, from another molar (Denisova 8) found in Denisova Cave in 2010. This new molar is similar to Denisova 4 in being very large and lacking traits typical of Neandertals and modern humans. Nuclear DNA sequences from the two molars form a clade with Denisova 3. The mtDNA of Denisova 8 is more diverged and has accumulated fewer substitutions than the mtDNAs of the other two specimens, suggesting Denisovans were present in the region over an extended period. The nuclear DNA sequence diversity among the three Denisovans is comparable to that among six Neandertals, but lower than that among present-day humans.}, }
@article {pmid26601491, year = {2015}, author = {Malyarchuk, BA and Derenko, MV and Denisova, GA and Litvinov, AN}, title = {[Topological Conflicts in Phylogenetic Analysis of Different Regions of the Sable (Martes zibellina L.) Mitochondrial Genome].}, journal = {Genetika}, volume = {51}, number = {8}, pages = {915-923}, pmid = {26601491}, issn = {0016-6758}, mesh = {Animals ; DNA, Mitochondrial/*genetics ; Genome, Mitochondrial/*genetics ; Haplotypes/genetics ; Mustelidae/*genetics ; Mutation ; *Phylogeny ; RNA, Ribosomal/genetics ; }, abstract = {Phylogenetic analysis of different regions of the mitochondrial genome of the sable showed the presence of several topologies of phylogenetic trees, but the most statistically significant topology is A-BC, which was obtained as a result of the analysis of the mitochondrial genome as a whole, as well as of the individual CO1, ND4, and ND5 genes. Analysis of the intergroup divergence of the mtDNA haplotypes (Dxy) indicated that the maximum Dxy values between A and BC groups were accompanied by minimum differences between B and C groups only for six genes showing the A-BC topology (12S rRNA; CO1, CO2, ND4, ND5, and CYTB). It is assumed that the topological conflicts observed in the analysis of individual sable mtDNA genes are associated with the uneven distribution of mutations along the mitochondrial genome and the mitochondrial tree. This may be due to random causes, as well as the nonuniform effect of selection.}, }
@article {pmid26596347, year = {2016}, author = {Racimo, F}, title = {Testing for Ancient Selection Using Cross-population Allele Frequency Differentiation.}, journal = {Genetics}, volume = {202}, number = {2}, pages = {733-750}, pmid = {26596347}, issn = {1943-2631}, support = {R01 GM040282/GM/NIGMS NIH HHS/United States ; R01-GM40282/GM/NIGMS NIH HHS/United States ; }, mesh = {Algorithms ; *Alleles ; Animals ; Asian Continental Ancestry Group/genetics ; Computer Simulation ; European Continental Ancestry Group/genetics ; Evolution, Molecular ; *Gene Frequency ; *Genetics, Population ; Genome-Wide Association Study ; Genomics/methods ; Humans ; *Models, Genetic ; Neanderthals/genetics ; ROC Curve ; *Selection, Genetic ; }, abstract = {A powerful way to detect selection in a population is by modeling local allele frequency changes in a particular region of the genome under scenarios of selection and neutrality and finding which model is most compatible with the data. A previous method based on a cross-population composite likelihood ratio (XP-CLR) uses an outgroup population to detect departures from neutrality that could be compatible with hard or soft sweeps, at linked sites near a beneficial allele. However, this method is most sensitive to recent selection and may miss selective events that happened a long time ago. To overcome this, we developed an extension of XP-CLR that jointly models the behavior of a selected allele in a three-population tree. Our method - called "3-population composite likelihood ratio" (3P-CLR) - outperforms XP-CLR when testing for selection that occurred before two populations split from each other and can distinguish between those events and events that occurred specifically in each of the populations after the split. We applied our new test to population genomic data from the 1000 Genomes Project, to search for selective sweeps that occurred before the split of Yoruba and Eurasians, but after their split from Neanderthals, and that could have led to the spread of modern-human-specific phenotypes. We also searched for sweep events that occurred in East Asians, Europeans, and the ancestors of both populations, after their split from Yoruba. In both cases, we are able to confirm a number of regions identified by previous methods and find several new candidates for selection in recent and ancient times. For some of these, we also find suggestive functional mutations that may have driven the selective events.}, }
@article {pmid26567083, year = {2016}, author = {Vyas, DN and Kitchen, A and Miró-Herrans, AT and Pearson, LN and Al-Meeri, A and Mulligan, CJ}, title = {Bayesian analyses of Yemeni mitochondrial genomes suggest multiple migration events with Africa and Western Eurasia.}, journal = {American journal of physical anthropology}, volume = {159}, number = {3}, pages = {382-393}, doi = {10.1002/ajpa.22890}, pmid = {26567083}, issn = {1096-8644}, support = {//Howard Hughes Medical Institute/United States ; }, mesh = {Africa ; Anthropology, Physical ; Asia, Western ; Bayes Theorem ; Europe ; Genome, Mitochondrial/*genetics ; Haplotypes ; History, Ancient ; *Human Migration ; Humans ; Phylogeny ; Yemen ; }, abstract = {OBJECTIVES: Anatomically, modern humans are thought to have migrated out of Africa ∼60,000 years ago in the first successful global dispersal. This initial migration may have passed through Yemen, a region that has experienced multiple migrations events with Africa and Eurasia throughout human history. We use Bayesian phylogenetics to determine how ancient and recent migrations have shaped Yemeni mitogenomic variation.<br><br>MATERIALS AND METHODS: We sequenced 113 mitogenomes from multiple Yemeni regions with a focus on haplogroups M, N, and L3(xM,N) as these groups have the oldest evolutionary history outside of Africa. We performed Bayesian evolutionary analyses to generate time-measured phylogenies calibrated by Neanderthal and Denisovan mitogenomes in order to determine the age of Yemeni-specific clades.<br><br>RESULTS: As defined by Yemeni monophyly, Yemeni in situ evolution is limited to the Holocene or latest Pleistocene (ages of clades in subhaplogroups L3b1a1a, L3h2, L3x1, M1a1f, M1a5, N1a1a3, and N1a3 range from 2 to 14 kya) and is often situated within broader Horn of Africa/southern Arabia in situ evolution (L3h2, L3x1, M1a1f, M1a5, and N1a1a3 ages range from 7 to 29 kya). Five subhaplogroups show no monophyly and are candidates for Holocene migration into Yemen (L0a2a2a, L3d1a1a, L3i2, M1a1b, and N1b1a).<br><br>DISCUSSION: Yemeni mitogenomes are largely the product of Holocene migration, and subsequent in situ evolution, from Africa and western Eurasia. However, we hypothesize that recent population movements may obscure the genetic signature of more ancient migrations. Additional research, e.g., analyses of Yemeni nuclear genetic data, is needed to better reconstruct the complex population and migration histories associated with Out of Africa.}, }
@article {pmid26525473, year = {2015}, author = {Soldatsky, YL and Denisova, OA and Mazur, EM}, title = {[Local anesthesia in the children undergoing the fibroendoscopic study of the nasal cavity, nasopharynx, and larynx: are topical anesthetics needed?].}, journal = {Vestnik otorinolaringologii}, volume = {80}, number = {5}, pages = {51-55}, doi = {10.17116/otorino201580551-55}, pmid = {26525473}, issn = {0042-4668}, mesh = {Adolescent ; Anesthesia, Local/methods/*standards ; *Anesthetics, Local/administration &amp; dosage/adverse effects/pharmacology ; Child ; Child, Preschool ; Double-Blind Method ; Endoscopy/methods/*standards ; Female ; Humans ; Larynx/drug effects ; Male ; *Nasal Cavity/drug effects ; *Nasopharynx/drug effects ; }, abstract = {This prospective randomized study with double blind control was designed to evaluate the effectiveness of various anesthetic techniques employed prior to fibroendoscopy of the nose, nasopharynx, and larynx of the children. The study included 160 children at the age varying from 3 to 14 (mean 7.4±2.96) years randomly allocated to four statistically comparable groups matched for age and sex. The following preparations were used to treat the children prior to fibroendoscopy: physiological solution (group 1), a 0.05% xylometazoline solution (group 2), a 10% lidocaine solution (group 3), and a mixture of 0.05% xylometazoline and 10% lidocaine solutions (group 4). The evaluation of the tolerance to the pretreatment of the nasal cavity with lidocaine and lidocaine plus xylometazoline (groups 3 and 4) showed that it was significantly (p<0.05) worse than in groups 1 and 2. The subjective tolerance to fibroendoscopy as reported by the patients was on the average similar in the children of all four groups (p>0.05). The doctors found the tolerance of fibroendoscopy to be the worst following pretreatment with the physiological solution (group 1) and the best after pretreatment with a mixture of lidocaine and xylometazoline (group 4) (p=0.03). The children comprising groups 2 and 3 were not significantly different in terms of the tolerance to fibroendoscopy (p>0.05). It is concluded that the pretreatment of the nasal cavity of the children with a 10% lidocaine solution before fibroendoscopy has no advantage over the pretreatment with a 0.05% xylometazoline solution; at the same time, insuflation of lidocaine as an anesthetic induces more pronounced negative emotions compared with the application of 0.05% xylometazoline.}, }
@article {pmid26455912, year = {2015}, author = {Bender, F and Gorbati, M and Cadavieco, MC and Denisova, N and Gao, X and Holman, C and Korotkova, T and Ponomarenko, A}, title = {Theta oscillations regulate the speed of locomotion via a hippocampus to lateral septum pathway.}, journal = {Nature communications}, volume = {6}, number = {}, pages = {8521}, pmid = {26455912}, issn = {2041-1723}, mesh = {Animals ; GABAergic Neurons/physiology ; Hippocampus/*physiology ; *Locomotion ; Male ; Mice, Inbred C57BL ; Mice, Transgenic ; *Optogenetics ; Random Allocation ; Septum of Brain/*physiology ; Spatial Navigation ; *Theta Rhythm ; }, abstract = {Hippocampal theta oscillations support encoding of an animal's position during spatial navigation, yet longstanding questions about their impact on locomotion remain unanswered. Combining optogenetic control of hippocampal theta oscillations with electrophysiological recordings in mice, we show that hippocampal theta oscillations regulate locomotion. In particular, we demonstrate that their regularity underlies more stable and slower running speeds during exploration. More regular theta oscillations are accompanied by more regular theta-rhythmic spiking output of pyramidal cells. Theta oscillations are coordinated between the hippocampus and its main subcortical output, the lateral septum (LS). Chemo- or optogenetic inhibition of this pathway reveals its necessity for the hippocampal regulation of running speed. Moreover, theta-rhythmic stimulation of LS projections to the lateral hypothalamus replicates the reduction of running speed induced by more regular hippocampal theta oscillations. These results suggest that changes in hippocampal theta synchronization are translated into rapid adjustment of running speed via the LS.}, }
@article {pmid26416425, year = {2015}, author = {Denisova, E and Heidenreich, B and Nagore, E and Rachakonda, PS and Hosen, I and Akrap, I and Traves, V and García-Casado, Z and López-Guerrero, JA and Requena, C and Sanmartin, O and Serra-Guillén, C and Llombart, B and Guillén, C and Ferrando, J and Gimeno, E and Nordheim, A and Hemminki, K and Kumar, R}, title = {Frequent DPH3 promoter mutations in skin cancers.}, journal = {Oncotarget}, volume = {6}, number = {34}, pages = {35922-35930}, pmid = {26416425}, issn = {1949-2553}, mesh = {Amino Acid Sequence ; Base Sequence ; Carcinoma, Squamous Cell/*genetics/pathology ; Carrier Proteins/*genetics ; Humans ; Intracellular Signaling Peptides and Proteins ; Melanoma/genetics/pathology ; Molecular Sequence Data ; Mutation ; Promoter Regions, Genetic ; Skin Neoplasms/*genetics/pathology ; }, abstract = {Recent reports suggested frequent occurrence of cancer associated somatic mutations within regulatory elements of the genome. Based on initial exome sequencing of 21 melanomas, we report frequent somatic mutations in skin cancers in a bidirectional promoter of diphthamide biosynthesis 3 (DPH3) and oxidoreductase NAD-binding domain containing 1 (OXNAD1) genes. The UV-signature mutations occurred at sites adjacent and within a binding motif for E-twenty six/ternary complex factors (Ets/TCF), at -8 and -9 bp from DPH3 transcription start site. Follow up screening of 586 different skin lesions showed that the DPH3 promoter mutations were present in melanocytic nevi (2/114; 2%), melanoma (30/304; 10%), basal cell carcinoma of skin (BCC; 57/137; 42%) and squamous cell carcinoma of skin (SCC; 12/31; 39%). Reporter assays carried out in one melanoma cell line for DPH3 and OXNAD1 orientations showed statistically significant increased promoter activity due to -8/-9CC > TT tandem mutations; although, no effect of the mutations on DPH3 and OXNAD1 transcription in tumors was observed. The results from this study show occurrence of frequent somatic non-coding mutations adjacent to a pre-existing binding site for Ets transcription factors within the directional promoter of DPH3 and OXNAD1 genes in three major skin cancers. The detected mutations displayed typical UV signature; however, the functionality of the mutations remains to be determined.}, }
@article {pmid26415272, year = {2015}, author = {Denisova, EV and Nazarov, VE}, title = {[PRINCIPLES OF POSTOPERATIVE DRUG THERAPY OF COMPLICATED DUODENAL ULCERS].}, journal = {Eksperimental'naia i klinicheskaia gastroenterologiia = Experimental &amp; clinical gastroenterology}, volume = {}, number = {4}, pages = {89-93}, pmid = {26415272}, issn = {1682-8658}, mesh = {Adult ; Anti-Bacterial Agents/administration &amp; dosage/therapeutic use ; Duodenal Ulcer/*complications/microbiology/*surgery ; Female ; Gastrointestinal Agents/administration &amp; dosage/therapeutic use ; Gastrointestinal Motility/drug effects ; Helicobacter Infections/drug therapy/microbiology ; Humans ; Male ; Middle Aged ; Postoperative Complications/*drug therapy/etiology/microbiology ; *Precision Medicine ; Treatment Outcome ; Young Adult ; }, abstract = {The article highlights the principles of individualized drug therapy of complicated duodenal ulcers in the postoperative period, based on the removal of the pathophysiological changes that occurred after different types of medical or surgical benefits.}, }
@article {pmid26399483, year = {2015}, author = {Rogers, RL}, title = {Chromosomal Rearrangements as Barriers to Genetic Homogenization between Archaic and Modern Humans.}, journal = {Molecular biology and evolution}, volume = {32}, number = {12}, pages = {3064-3078}, pmid = {26399483}, issn = {1537-1719}, support = {R01 GM040282/GM/NIGMS NIH HHS/United States ; R01-GM40282/GM/NIGMS NIH HHS/United States ; }, mesh = {Animals ; Biological Evolution ; DNA Transposable Elements ; Evolution, Molecular ; Female ; Fossils ; Gene Flow/genetics ; *Gene Rearrangement ; Genetic Variation ; *Genome, Human ; Hominidae/*genetics ; Humans ; Male ; Neanderthals/*genetics ; Phylogeny ; Polymorphism, Genetic ; Recombination, Genetic/genetics ; Sequence Analysis, DNA ; Y Chromosome ; }, abstract = {Chromosomal rearrangements, which shuffle DNA throughout the genome, are an important source of divergence across taxa. Using a paired-end read approach with Illumina sequence data for archaic humans, I identify changes in genome structure that occurred recently in human evolution. Hundreds of rearrangements indicate genomic trafficking between the sex chromosomes and autosomes, raising the possibility of sex-specific changes. Additionally, genes adjacent to genome structure changes in Neanderthals are associated with testis-specific expression, consistent with evolutionary theory that new genes commonly form with expression in the testes. I identify one case of new-gene creation through transposition from the Y chromosome to chromosome 10 that combines the 5'-end of the testis-specific gene Fank1 with previously untranscribed sequence. This new transcript experienced copy number expansion in archaic genomes, indicating rapid genomic change. Among rearrangements identified in Neanderthals, 13% are transposition of selfish genetic elements, whereas 32% appear to be ectopic exchange between repeats. In Denisovan, the pattern is similar but numbers are significantly higher with 18% of rearrangements reflecting transposition and 40% ectopic exchange between distantly related repeats. There is an excess of divergent rearrangements relative to polymorphism in Denisovan, which might result from nonuniform rates of mutation, possibly reflecting a burst of transposable element activity in the lineage that led to Denisovan. Finally, loci containing genome structure changes show diminished rates of introgression from Neanderthals into modern humans, consistent with the hypothesis that rearrangements serve as barriers to gene flow during hybridization. Together, these results suggest that this previously unidentified source of genomic variation has important biological consequences in human evolution.}, }
@article {pmid26387173, year = {2015}, author = {Denisova, OA and Livzan, MA}, title = {[THE POSSIBILITY OF USING ALGINATE TEST IN THE DIAGNOSIS GASTROESOPHAGEAL REFLUX DISEASE].}, journal = {Eksperimental'naia i klinicheskaia gastroenterologiia = Experimental &amp; clinical gastroenterology}, volume = {}, number = {5}, pages = {67-70}, pmid = {26387173}, issn = {1682-8658}, mesh = {Adult ; Age Factors ; Aged ; Aged, 80 and over ; *Alginates ; Endoscopy, Digestive System/*methods ; Female ; Gastroesophageal Reflux/*diagnosis/metabolism ; Glucuronic Acid ; Hexuronic Acids ; Humans ; Hydrogen-Ion Concentration ; Male ; Middle Aged ; }, abstract = {UNLABELLED: Currently, gastroesophageal reflux disease (GERD) in the elderly has become an issue of gastroenterology. To date, however, no reference criteria endoscopically negative forms of GERD, therefore, the most significant factor in diagnosis is the detection of gastroesophageal reflux.<br><br>PURPOSE: To estimate the information content of alginate test in the diagnosis of GERD in the age aspect.<br><br>MATERIALS AND METHODS: Core group--those aged 60-86 years, the comparative group 25-59 years. Two subgroups: the results of alginate test with a positive result and a negative result. Calculated the body mass index, endoscopic examination was performed daily pH--metry.<br><br>RESULTS: There is direct correlation between the test result and clinical and instrumental signs of GERD. Alginate test can be used as a screening method for the early diagnosis in patients with GERD different age groups.}, }
@article {pmid26325393, year = {2016}, author = {Denisova, K and Feldman, J and Su, X and Singh, M}, title = {Investigating shape representation using sensitivity to part- and axis-based transformations.}, journal = {Vision research}, volume = {126}, number = {}, pages = {347-361}, pmid = {26325393}, issn = {1878-5646}, support = {R01 EY021494/EY/NEI NIH HHS/United States ; }, mesh = {*Discrimination, Psychological ; *Form Perception ; Humans ; Judgment ; Photic Stimulation/methods ; Psychometrics ; Rotation ; Sensory Thresholds ; *Visual Perception ; }, abstract = {Part- and axis-based approaches organize shape representations in terms of simple parts and their spatial relationships. Shape transformations that alter qualitative part structure have been shown to be more detectable than those that preserve it. We compared sensitivity to various transformations that change quantitative properties of parts and their spatial relationships, while preserving qualitative part structure. Shape transformations involving changes in length, width, curvature, orientation and location were applied to a small part attached to a larger base of a two-part shape. Increment thresholds were estimated for each transformation using a 2IFC procedure. Thresholds were converted into common units of shape difference to enable comparisons across transformations. Higher sensitivity was consistently found for transformations involving a parameter of a single part (length, width, curvature) than those involving spatial relations between two parts (relative orientation and location), suggesting a single-part superiority effect. Moreover, sensitivity to shifts in part location - a biomechanically implausible shape transformation - was consistently poorest. The influence of region-based geometry was investigated via stereoscopic manipulation of figure and ground. Sensitivity was compared across positive parts (protrusions) and negative parts (indentations) for transformations involving a change in orientation or location. For changes in part orientation (biomechanically plausible), sensitivity was better for positive than negative parts; whereas for changes in part location (biomechanically implausible), no systematic difference was observed.}, }
@article {pmid26281203, year = {2015}, author = {Rekhtina, IG and Zakharova, EV and Stolyarevich, ES and Sinitsina, MN and Denisova, EN}, title = {[The concurrence of light-chain deposition disease, AL-amyloidosis, and cast nephropathy in a patient with multiple myeloma].}, journal = {Terapevticheskii arkhiv}, volume = {87}, number = {6}, pages = {98-101}, doi = {10.17116/terarkh201587698-101}, pmid = {26281203}, issn = {0040-3660}, mesh = {Amyloidosis/*complications/diagnosis ; Fatal Outcome ; Humans ; Immunoglobulin Light Chains/*metabolism ; Immunoglobulin Light-chain Amyloidosis ; Kidney Diseases/*complications/diagnosis ; Male ; Middle Aged ; Multiple Myeloma/*complications/diagnosis ; Paraproteinemias/*complications/diagnosis/metabolism ; }, abstract = {Despite of the fact that their clinical manifestations are similar, AL-amyloidosis (AL-A) and light chain deposition disease (LCDD) are individual nosological entities in view of considerable differences in their pathogenesis and pathomorphology. The paper describes a rare case of the concurrence of LCDD and AL-A in a patient with multiple myeloma. Clinically, there was dialysis-dependent renal failure, flail leg syndrome, myocardiopathy, and rhabdomyolysis. At the disease onset, his nephrobiopsy specimen could diagnose LCDD and myeloma or cast nephropathy. The disease was characterized by an aggressive course. Despite the administration of innovative agents, the patient had a short-term remission and died from disease progression. Autopsy additionally revealed amyloid deposition in the heart and kidney. The development of AL-A in the presence of prior LCDD may reflect the progression of the tumor and the appearance of an additional subclone of plasma cells that produce amyloidogenic light chains. The uncommonness of this case is that renal amyloid was found in the tubular casts and absent in the glomeruli, which may be considered as a special form--tubular AL-amyloidosis.}, }
@article {pmid26249230, year = {2015}, author = {Sudmant, PH and Mallick, S and Nelson, BJ and Hormozdiari, F and Krumm, N and Huddleston, J and Coe, BP and Baker, C and Nordenfelt, S and Bamshad, M and Jorde, LB and Posukh, OL and Sahakyan, H and Watkins, WS and Yepiskoposyan, L and Abdullah, MS and Bravi, CM and Capelli, C and Hervig, T and Wee, JT and Tyler-Smith, C and van Driem, G and Romero, IG and Jha, AR and Karachanak-Yankova, S and Toncheva, D and Comas, D and Henn, B and Kivisild, T and Ruiz-Linares, A and Sajantila, A and Metspalu, E and Parik, J and Villems, R and Starikovskaya, EB and Ayodo, G and Beall, CM and Di Rienzo, A and Hammer, MF and Khusainova, R and Khusnutdinova, E and Klitz, W and Winkler, C and Labuda, D and Metspalu, M and Tishkoff, SA and Dryomov, S and Sukernik, R and Patterson, N and Reich, D and Eichler, EE}, title = {Global diversity, population stratification, and selection of human copy-number variation.}, journal = {Science (New York, N.Y.)}, volume = {349}, number = {6253}, pages = {aab3761}, pmid = {26249230}, issn = {1095-9203}, support = {5DP1ES022577 05/DP/NCCDPHP CDC HHS/United States ; HHSN26120080001E//PHS HHS/International ; 1R01DK104339-01/DK/NIDDK NIH HHS/United States ; 261213//European Research Council/International ; R01 DK104339/DK/NIDDK NIH HHS/United States ; R01 HL119577/HL/NHLBI NIH HHS/United States ; /ImNIH/Intramural NIH HHS/United States ; 1R01GM113657-01/GM/NIGMS NIH HHS/United States ; P30 ES013508/ES/NIEHS NIH HHS/United States ; 098051//Wellcome Trust/United Kingdom ; /HHMI/Howard Hughes Medical Institute/United States ; R01 GM113657/GM/NIGMS NIH HHS/United States ; T32 GM007266/GM/NIGMS NIH HHS/United States ; 2R01HG002385/HG/NHGRI NIH HHS/United States ; R01 HG002385/HG/NHGRI NIH HHS/United States ; DP1 ES022577/ES/NIEHS NIH HHS/United States ; }, mesh = {African Continental Ancestry Group/classification/genetics ; Animals ; *DNA Copy Number Variations ; *Evolution, Molecular ; *Gene Duplication ; Genome, Human/*genetics ; Hominidae/genetics ; Humans ; Oceanic Ancestry Group/classification/genetics ; Phylogeny ; Polymorphism, Single Nucleotide ; Population/*genetics ; Selection, Genetic ; *Sequence Deletion ; }, abstract = {In order to explore the diversity and selective signatures of duplication and deletion human copy-number variants (CNVs), we sequenced 236 individuals from 125 distinct human populations. We observed that duplications exhibit fundamentally different population genetic and selective signatures than deletions and are more likely to be stratified between human populations. Through reconstruction of the ancestral human genome, we identify megabases of DNA lost in different human lineages and pinpoint large duplications that introgressed from the extinct Denisova lineage now found at high frequency exclusively in Oceanic populations. We find that the proportion of CNV base pairs to single-nucleotide-variant base pairs is greater among non-Africans than it is among African populations, but we conclude that this difference is likely due to unique aspects of non-African population history as opposed to differences in CNV load.}, }
@article {pmid26206078, year = {2015}, author = {Kutzner, A and Pramanik, S and Kim, PS and Heese, K}, title = {All-or-(N)One - an epistemological characterization of the human tumorigenic neuronal paralogous FAM72 gene loci.}, journal = {Genomics}, volume = {106}, number = {5}, pages = {278-285}, doi = {10.1016/j.ygeno.2015.07.003}, pmid = {26206078}, issn = {1089-8646}, mesh = {Animals ; Carrier Proteins/*genetics ; Chromosomes, Human, Pair 1 ; Computer Simulation ; Genes ; Genetic Loci ; Genomics ; Hominidae/genetics ; Humans ; Membrane Proteins ; Neanderthals/genetics ; Neoplasm Proteins/*genetics ; Phylogeny ; Proteins ; Segmental Duplications, Genomic ; }, abstract = {FAM72 is a novel neuronal progenitor cell (NPC) self-renewal supporting protein expressed under physiological conditions at low levels in other tissues. Accumulating data indicate the potential pivotal tumourigenic effects of FAM72. Our in silico human genome-wide analysis (GWA) revealed that the FAM72 gene family consists of four human-specific paralogous members, all of which are located on chromosome (chr) 1. Unique asymmetric FAM72 segmental gene duplications are most likely to have occurred in conjunction with the paired genomic neighbour SRGAP2 (SLIT-ROBO Rho GTPase activating protein), as both genes have four paralogues in humans but only one vertebra-emerging orthologue in all other species. No species with two or three FAM72/SRGAP2 gene pairs could be identified, and the four exclusively human-defining ohnologues, with different mutation patterns in Homo neanderthalensis and Denisova hominin, may remain under epigenetic control through long non-coding (lnc) RNAs.}, }
@article {pmid26176239, year = {2015}, author = {Blank, M and Blank, O and Myasnikova, E and Denisova, D}, title = {Peculiarities of Anxiety Score Distribution in Adult Cancer Patients.}, journal = {Journal of psychosocial oncology}, volume = {33}, number = {5}, pages = {551-560}, doi = {10.1080/07347332.2015.1067280}, pmid = {26176239}, issn = {1540-7586}, mesh = {Adult ; Antineoplastic Agents/therapeutic use ; Anxiety/*psychology ; Case-Control Studies ; Female ; Humans ; Male ; Neoplasms/drug therapy/*psychology ; Pregnancy ; Psychiatric Status Rating Scales ; Remission Induction ; }, abstract = {The goal of the present research is to investigate and analyze possible peculiarities of the psychological state of cancer patients undergoing treatment. Scores characterizing the trait and state anxiety were acquired using the Integrative Anxiety Test from four groups: adults with no appreciable disease, pregnant women, cancer patients examined during the specific antitumor treatment, and cancer patients brought into lasting clinical remission. Statistical analysis of the testing results revealed the bimodal type of the distribution of scores. The only statistically significant exception was the distribution of the state anxiety scores in cancer patients undergoing treatment that was clearly unimodal.}, }
@article {pmid26156123, year = {2015}, author = {Fish, I and Boissinot, S}, title = {Contrasted patterns of variation and evolutionary convergence at the antiviral OAS1 gene in old world primates.}, journal = {Immunogenetics}, volume = {67}, number = {9}, pages = {487-499}, pmid = {26156123}, issn = {1432-1211}, support = {P51 OD011133/OD/NIH HHS/United States ; P51 RR013986/RR/NCRR NIH HHS/United States ; }, mesh = {2',5'-Oligoadenylate Synthetase/*genetics ; Amino Acid Sequence ; Animals ; Base Sequence ; Disease Resistance/*genetics ; Evolution, Molecular ; Haplotypes/*genetics ; Macaca fascicularis/genetics/immunology ; Macaca mulatta/genetics/immunology ; Papio anubis/genetics/immunology ; Papio papio/genetics/immunology ; Polymorphism, Genetic/*genetics ; Protein Structure, Tertiary ; Sequence Alignment ; Sequence Analysis, DNA ; }, abstract = {The oligoadenylate synthetase 1 (OAS1) enzyme acts as an innate sensor of viral infection and plays a major role in the defense against a wide diversity of viruses. Polymorphisms at OAS1 have been shown to correlate with differential susceptibility to several infections of great public health significance, including hepatitis C virus, SARS coronavirus, and West Nile virus. Population genetics analyses in hominoids have revealed interesting evolutionary patterns. In Central African chimpanzee, OAS1 has evolved under long-term balancing selection, resulting in the persistence of polymorphisms since the origin of hominoids, whereas human populations have acquired and retained OAS1 alleles from Neanderthal and Denisovan origin. We decided to further investigate the evolution of OAS1 in primates by characterizing intra-specific variation in four species commonly used as models in infectious disease research: the rhesus macaque, the cynomolgus macaque, the olive baboon, and the Guinea baboon. In baboons, OAS1 harbors a very low level of variation. In contrast, OAS1 in macaques exhibits a level of polymorphism far greater than the genomic average, which is consistent with the action of balancing selection. The region of the enzyme that directly interacts with viral RNA, the RNA-binding domain, contains a number of polymorphisms likely to affect the RNA-binding affinity of OAS1. This strongly suggests that pathogen-driven balancing selection acting on the RNA-binding domain of OAS1 is maintaining variation at this locus. Interestingly, we found that a number of polymorphisms involved in RNA-binding were shared between macaques and chimpanzees. This represents an unusual case of convergent polymorphism.}, }
@article {pmid26122933, year = {2015}, author = {VanSeggelen, H and Hammill, JA and Dvorkin-Gheva, A and Tantalo, DG and Kwiecien, JM and Denisova, GF and Rabinovich, B and Wan, Y and Bramson, JL}, title = {T Cells Engineered With Chimeric Antigen Receptors Targeting NKG2D Ligands Display Lethal Toxicity in Mice.}, journal = {Molecular therapy : the journal of the American Society of Gene Therapy}, volume = {23}, number = {10}, pages = {1600-1610}, pmid = {26122933}, issn = {1525-0024}, support = {//Canadian Institutes of Health Research/Canada ; }, mesh = {Adoptive Transfer ; Animals ; Cell Line, Tumor ; Cell Membrane/metabolism ; Cyclophosphamide/pharmacology ; Cytokines/metabolism ; *Cytotoxicity, Immunologic ; Disease Models, Animal ; Female ; Gene Expression ; Genetic Vectors/genetics ; Immunotherapy, Adoptive ; Ligands ; Mice ; NK Cell Lectin-Like Receptor Subfamily K/metabolism ; Neoplasms/genetics/immunology/pathology/therapy ; Pneumonia/immunology/metabolism/pathology ; Receptors, Antigen, T-Cell/genetics/metabolism ; Receptors, Immunologic/genetics/metabolism ; *Recombinant Fusion Proteins ; Retroviridae/genetics ; T-Lymphocytes/immunology/metabolism ; Transplantation Conditioning ; }, abstract = {Ligands for the NKG2D receptor are overexpressed on tumors, making them interesting immunotherapy targets. To assess the tumoricidal properties of T cells directed to attack NKG2D ligands, we engineered murine T cells with two distinct NKG2D-based chimeric antigen receptors (CARs): (i) a fusion between the NKG2D receptor and the CD3ζ chain and (ii) a conventional second-generation CAR, where the extracellular domain of NKG2D was fused to CD28 and CD3ζ. To enhance the CAR surface expression, we also engineered T cells to coexpress DAP10. In vitro functionality and surface expression levels of all three CARs was greater in BALB/c T cells than C57BL/6 T cells, indicating strain-specific differences. Upon adoptive transfer of NKG2D-CAR-T cells into syngeneic animals, we observed significant clinical toxicity resulting in morbidity and mortality. The severity of these toxicities varied between the CAR configurations and paralleled their in vitro NKG2D surface expression. BALB/c mice were more sensitive to these toxicities than C57BL/6 mice, consistent with the higher in vitro functionality of BALB/c T cells. Treatment with cyclophosphamide prior to adoptive transfer exacerbated the toxicity. We conclude that while NKG2D ligands may be useful targets for immunotherapy, the pursuit of NKG2D-based CAR-T cell therapies should be undertaken with caution.}, }
@article {pmid26104010, year = {2015}, author = {Qin, P and Stoneking, M}, title = {Denisovan Ancestry in East Eurasian and Native American Populations.}, journal = {Molecular biology and evolution}, volume = {32}, number = {10}, pages = {2665-2674}, doi = {10.1093/molbev/msv141}, pmid = {26104010}, issn = {1537-1719}, mesh = {Animals ; Consanguinity ; Gene Flow ; *Genealogy and Heraldry ; Geography ; Hominidae/genetics ; Humans ; Indians, North American/*genetics ; Models, Genetic ; Oceanic Ancestry Group/genetics ; Siberia ; }, abstract = {Although initial studies suggested that Denisovan ancestry was found only in modern human populations from island Southeast Asia and Oceania, more recent studies have suggested that Denisovan ancestry may be more widespread. However, the geographic extent of Denisovan ancestry has not been determined, and moreover the relationship between the Denisovan ancestry in Oceania and that elsewhere has not been studied. Here we analyze genome-wide single nucleotide polymorphism data from 2,493 individuals from 221 worldwide populations, and show that there is a widespread signal of a very low level of Denisovan ancestry across Eastern Eurasian and Native American (EE/NA) populations. We also verify a higher level of Denisovan ancestry in Oceania than that in EE/NA; the Denisovan ancestry in Oceania is correlated with the amount of New Guinea ancestry, but not the amount of Australian ancestry, indicating that recent gene flow from New Guinea likely accounts for signals of Denisovan ancestry across Oceania. However, Denisovan ancestry in EE/NA populations is equally correlated with their New Guinea or their Australian ancestry, suggesting a common source for the Denisovan ancestry in EE/NA and Oceanian populations. Our results suggest that Denisovan ancestry in EE/NA is derived either from common ancestry with, or gene flow from, the common ancestor of New Guineans and Australians, indicating a more complex history involving East Eurasians and Oceanians than previously suspected.}, }
@article {pmid26081318, year = {2015}, author = {Katamanova, EV and Rukavishnikov, VS and Lakhman, OL and Shevchenko, OI and Denisova, IA}, title = {[Cognitive impairment in a toxic lesion of the brain].}, journal = {Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova}, volume = {115}, number = {2}, pages = {11-15}, doi = {10.17116/jnevro20151152111-15}, pmid = {26081318}, issn = {1997-7298}, mesh = {Alcoholism/*complications/physiopathology ; Brain/*pathology ; Brain Diseases/*complications/diagnosis/physiopathology ; Cognition/*physiology ; Cognition Disorders/diagnosis/*etiology/physiopathology ; Electroencephalography ; Female ; Follow-Up Studies ; Humans ; Magnetic Resonance Imaging ; Male ; Memory/*physiology ; Mercury Poisoning, Nervous System/*complications/physiopathology ; Middle Aged ; Neuropsychological Tests ; Severity of Illness Index ; Time Factors ; }, abstract = {OBJECTIVE: To identify features of cognitive impairment in patients with toxic (mercury or alcohol) encephalopathy.<br><br>MATERIAL AND METHODS: The study involved 36 patients with chronic mercury intoxication and 30 people with chronic alcoholism. A control group included 30 age-matched healthy men who were not exposed to toxic substances and alcohol abuse. All patients underwent neuropsychological examination, which involved a set of neuropsychological Luria rated memory status, praxis, gnosis and speeches. MMSE and FAB were used for the diagnosis of moderate cognitive impairment. Computer electroencephalography and cognitive evoked potentials method were used as well.<br><br>RESULTS AND CONCLUSION: The diffuse brain injury in toxic encephalopathy (alcohol and mercury) on EEG, and according to the results of neuropsychological testing was identified. Changes in analytical and synthetic thinking, audio-verbal, long-term, visual memory, reciprocal coordination, finger gnosis, impressive speech were observed in mercury encephalopathy. Functional failure of the frontal lobe and the premotor area of the left hemisphere were revealed in alcoholic encephalopathy.}, }
@article {pmid26080582, year = {2015}, author = {Zavgorodnyaya, NG and Denisova, OO}, title = {[Surgical treatment for optic neuropathy in high myopia].}, journal = {Vestnik oftalmologii}, volume = {131}, number = {2}, pages = {45-49}, doi = {10.17116/oftalma2015131245-49}, pmid = {26080582}, issn = {0042-465X}, mesh = {Adult ; Collagen/therapeutic use ; Diagnostic Techniques, Ophthalmological ; Female ; Follow-Up Studies ; Humans ; Male ; *Myopia/complications/diagnosis ; *Optic Nerve Diseases/diagnosis/etiology/surgery ; Prostheses and Implants ; *Scleroplasty/instrumentation/methods ; Treatment Outcome ; }, abstract = {AIM: To improve the effectiveness of surgical treatment for optic neuropathy in high-degree myopia.<br><br>MATERIAL AND METHODS: A total of 54 patients (96 eyes) aged 18-30 with high myopia and signs of optic neuropathy were observed. The main group consisted of 20 patients (28 eyes) who underwent meridional scleral reinforcement with type I collagen-based implant placed over the posterior segment. The control group included 34 patients (68 eyes) who were given a 10-day vitamin and vascular therapy course. In most of the controls (21 patient, 21 eyes) indirect revascularization with ligation of the superficial temporal artery was performed. Ophthalmological examination was done before the beginning of the treatment and then at 1, 6, and 12 months. Results. At the end of the follow-up period best corrected visual acuity in the main group was 19.2% higher, on average, than baseline values, but remained unchanged in the controls. Light sensitivity of the retina in both group increased by 7% and 2% respectively. Electrically evoked phosphene thresholds decreased by 22.5% and 10.4% respectively. The main group also demonstrated a near-constant mean peripapillary nerve fiber layer (RNFL) thickness and a 16.4% higher than baseline blood flow velocity in the ophthalmic artery. In the control group the latter parameter showed no change.<br><br>CONCLUSIONS: Scleral reinforcement surgery in patients with high myopia complicated by optic neuropathy enables improvement of visual functions and regional blood flow as well as stabilization of RNFL thickness and volume, thus, preventing subsequent development of optic nerve atrophy.}, }
@article {pmid26073780, year = {2015}, author = {Lou, H and Lu, Y and Lu, D and Fu, R and Wang, X and Feng, Q and Wu, S and Yang, Y and Li, S and Kang, L and Guan, Y and Hoh, BP and Chung, YJ and Jin, L and Su, B and Xu, S}, title = {A 3.4-kb Copy-Number Deletion near EPAS1 Is Significantly Enriched in High-Altitude Tibetans but Absent from the Denisovan Sequence.}, journal = {American journal of human genetics}, volume = {97}, number = {1}, pages = {54-66}, pmid = {26073780}, issn = {1537-6605}, mesh = {Adaptation, Biological/*genetics ; Algorithms ; *Altitude ; Animals ; Base Sequence ; Basic Helix-Loop-Helix Transcription Factors/*genetics ; DNA Copy Number Variations/*genetics ; Ethnic Groups/*genetics ; *Evolution, Molecular ; Genetics, Population ; Hemoglobins/genetics/metabolism ; Hominidae/*genetics ; Humans ; Linkage Disequilibrium ; Microarray Analysis/methods ; Molecular Sequence Data ; Polymerase Chain Reaction/methods ; Sequence Analysis, DNA ; Tibet ; }, abstract = {Tibetan high-altitude adaptation (HAA) has been studied extensively, and many candidate genes have been reported. Subsequent efforts targeting HAA functional variants, however, have not been that successful (e.g., no functional variant has been suggested for the top candidate HAA gene, EPAS1). With WinXPCNVer, a method developed in this study, we detected in microarray data a Tibetan-enriched deletion (TED) carried by 90% of Tibetans; 50% were homozygous for the deletion, whereas only 3% carried the TED and 0% carried the homozygous deletion in 2,792 worldwide samples (p < 10(-15)). We employed long PCR and Sanger sequencing technologies to determine the exact copy number and breakpoints of the TED in 70 additional Tibetan and 182 diverse samples. The TED had identical boundaries (chr2: 46,694,276-46,697,683; hg19) and was 80 kb downstream of EPAS1. Notably, the TED was in strong linkage disequilibrium (LD; r(2) = 0.8) with EPAS1 variants associated with reduced blood concentrations of hemoglobin. It was also in complete LD with the 5-SNP motif, which was suspected to be introgressed from Denisovans, but the deletion itself was absent from the Denisovan sequence. Correspondingly, we detected that footprints of positive selection for the TED occurred 12,803 (95% confidence interval = 12,075-14,725) years ago. We further whole-genome deep sequenced (>60×) seven Tibetans and verified the TED but failed to identify any other copy-number variations with comparable patterns, giving this TED top priority for further study. We speculate that the specific patterns of the TED resulted from its own functionality in HAA of Tibetans or LD with a functional variant of EPAS1.}, }
@article {pmid26072518, year = {2015}, author = {Hoover, KC and Gokcumen, O and Qureshy, Z and Bruguera, E and Savangsuksa, A and Cobb, M and Matsunami, H}, title = {Global Survey of Variation in a Human Olfactory Receptor Gene Reveals Signatures of Non-Neutral Evolution.}, journal = {Chemical senses}, volume = {40}, number = {7}, pages = {481-488}, pmid = {26072518}, issn = {1464-3553}, support = {DC005782/DC/NIDCD NIH HHS/United States ; }, mesh = {Alleles ; Base Sequence ; DNA/genetics ; *Evolution, Molecular ; Genetic Variation/*genetics ; Humans ; Polymorphism, Single Nucleotide/genetics ; Receptors, Odorant/*genetics ; }, abstract = {Allelic variation at 4 loci in the human olfactory receptor gene OR7D4 is associated with perceptual variation in the sex steroid-derived odorants, androstenone, and androstadienone. Androstadienone has been linked with chemosensory identification whereas androstenone makes pork from uncastrated pigs distasteful ("boar taint"). In a sample of 2224 individuals from 43 populations, we identified 45 OR7D4 single nucleotide polymorphisms. Coalescent modeling of frequency-site-spectrum-based statistics identified significant deviation from neutrality in human OR7D4; individual populations with statistically significant deviations from neutrality include Gujarati, Beijing Han, Great Britain, Iberia, and Puerto Rico. Analysis of molecular variation values indicated statistically significant population differentiation driven mainly by the 4 alleles associated with androstenone perception variation; however, fixation values were low suggesting that genetic structure may not have played a strong role in creating these group divisions. We also studied OR7D4 in the genomes of extinct members of the human lineage: Altai Neandertal and Denisovan. No variants were identified in Altai but 2 were in Denisova, one of which is shared by modern humans and one of which is novel. A functional test of modern human and a synthesized mutant Denisova OR7D4 indicated no statistically significant difference in responses to androstenone between the 2 species. Our results suggest non-neutral evolution for an olfactory receptor gene.}, }
@article {pmid26017008, year = {2015}, author = {Fontes, JD and Ramsey, J and Polk, JM and Koop, A and Denisova, JV and Belousov, AB}, title = {Death of Neurons following Injury Requires Conductive Neuronal Gap Junction Channels but Not a Specific Connexin.}, journal = {PloS one}, volume = {10}, number = {5}, pages = {e0125395}, pmid = {26017008}, issn = {1932-6203}, support = {P20 GM104936/GM/NIGMS NIH HHS/United States ; R21 NS076925/NS/NINDS NIH HHS/United States ; P30 AG035982/AG/NIA NIH HHS/United States ; HD002528/HD/NICHD NIH HHS/United States ; UL1 TR000001/TR/NCATS NIH HHS/United States ; R21NS076925/NS/NINDS NIH HHS/United States ; P30 HD002528/HD/NICHD NIH HHS/United States ; }, mesh = {Animals ; Blotting, Western ; Cell Death/genetics/physiology ; Cell Survival/genetics/physiology ; Cells, Cultured ; Connexins/genetics/metabolism ; Gap Junctions/*metabolism ; Genetic Vectors/genetics ; HeLa Cells ; Humans ; Lentivirus/genetics ; Mice ; Mice, Knockout ; Receptors, N-Methyl-D-Aspartate/genetics/metabolism ; }, abstract = {Pharmacological blockade or genetic knockout of neuronal connexin 36 (Cx36)-containing gap junctions reduces neuronal death caused by ischemia, traumatic brain injury and NMDA receptor (NMDAR)-mediated excitotoxicity. However, whether Cx36 gap junctions contribute to neuronal death via channel-dependent or channel-independent mechanism remains an open question. To address this, we manipulated connexin protein expression via lentiviral transduction of mouse neuronal cortical cultures and analyzed neuronal death twenty-four hours following administration of NMDA (a model of NMDAR excitotoxicity) or oxygen-glucose deprivation (a model of ischemic injury). In cultures prepared from wild-type mice, over-expression and knockdown of Cx36-containing gap junctions augmented and prevented, respectively, neuronal death from NMDAR-mediated excitotoxicity and ischemia. In cultures obtained form from Cx36 knockout mice, re-expression of functional gap junction channels, containing either neuronal Cx36 or non-neuronal Cx43 or Cx31, resulted in increased neuronal death following insult. In contrast, the expression of communication-deficient gap junctions (containing mutated connexins) did not have this effect. Finally, the absence of ethidium bromide uptake in non-transduced wild-type neurons two hours following NMDAR excitotoxicity or ischemia suggested the absence of active endogenous hemichannels in those neurons. Taken together, these results suggest a role for neuronal gap junctions in cell death via a connexin type-independent mechanism that likely relies on channel activities of gap junctional complexes among neurons. A possible contribution of gap junction channel-permeable death signals in neuronal death is discussed.}, }
@article {pmid26000734, year = {2015}, author = {Kari, L and Hill, KA and Sayem, AS and Karamichalis, R and Bryans, N and Davis, K and Dattani, NS}, title = {Mapping the space of genomic signatures.}, journal = {PloS one}, volume = {10}, number = {5}, pages = {e0119815}, pmid = {26000734}, issn = {1932-6203}, mesh = {Animals ; DNA, Mitochondrial/*genetics ; *Models, Theoretical ; }, abstract = {We propose a computational method to measure and visualize interrelationships among any number of DNA sequences allowing, for example, the examination of hundreds or thousands of complete mitochondrial genomes. An "image distance" is computed for each pair of graphical representations of DNA sequences, and the distances are visualized as a Molecular Distance Map: Each point on the map represents a DNA sequence, and the spatial proximity between any two points reflects the degree of structural similarity between the corresponding sequences. The graphical representation of DNA sequences utilized, Chaos Game Representation (CGR), is genome- and species-specific and can thus act as a genomic signature. Consequently, Molecular Distance Maps could inform species identification, taxonomic classifications and, to a certain extent, evolutionary history. The image distance employed, Structural Dissimilarity Index (DSSIM), implicitly compares the occurrences of oligomers of length up to k (herein k = 9) in DNA sequences. We computed DSSIM distances for more than 5 million pairs of complete mitochondrial genomes, and used Multi-Dimensional Scaling (MDS) to obtain Molecular Distance Maps that visually display the sequence relatedness in various subsets, at different taxonomic levels. This general-purpose method does not require DNA sequence alignment and can thus be used to compare similar or vastly different DNA sequences, genomic or computer-generated, of the same or different lengths. We illustrate potential uses of this approach by applying it to several taxonomic subsets: phylum Vertebrata, (super)kingdom Protista, classes Amphibia-Insecta-Mammalia, class Amphibia, and order Primates. This analysis of an extensive dataset confirms that the oligomer composition of full mtDNA sequences can be a source of taxonomic information. This method also correctly finds the mtDNA sequences most closely related to that of the anatomically modern human (the Neanderthal, the Denisovan, and the chimp), and that the sequence most different from it in this dataset belongs to a cucumber.}, }
@article {pmid25956332, year = {2015}, author = {Solovyeva, EV and Myund, LA and Denisova, AS}, title = {Surface enhanced Raman scattering of new acridine based fluorophore adsorbed on silver electrode.}, journal = {Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy}, volume = {149}, number = {}, pages = {196-200}, doi = {10.1016/j.saa.2015.04.092}, pmid = {25956332}, issn = {1873-3557}, abstract = {4,5-Bis(N,N-di(2-hydroxyethyl)iminomethyl)acridine (BHIA) is a new acridine based fluoroionophore and a highly-selective sensor for cadmium ion. The direct interaction of the aromatic nitrogen atom with a surface is impossible since there are bulky substituents in the 4,5-positions of the acridine fragment. Nevertheless BHIA molecule shows a reliable SERS spectrum while adsorbed on a silver electrode. The analysis of SERS spectra pH dependence reveals that BHIA species adsorbed on a surface can exist in both non-protonated and protonated forms. The adsorption of BHIA from alkaline solution is accompanied by carbonaceous species formation at the surface. The intensity of such "carbon bands" turned out to be related with the supporting electrolyte (KCl) concentration. Upon lowering the electrode potential the SERS spectra of BHIA do not undergo changes but the intensity of bands decreases. This indicates that the adsorption mechanism on the silver surface is realized via aromatic system of acridine fragment. In case of such an adsorption mechanism the chelate fragment of the BHIA molecule is capable of interaction with the solution components. Addition of Cd(2+) ions to a system containing BHIA adsorbed on a silver electrode in equilibrium with the solution leads to the formation of BHIA/Cd(2+) complex which desorption causes the loss of SERS signal.}, }
@article {pmid25857197, year = {2015}, author = {Maliarchuk, BA and Derenko, MV and Denisova, GA}, title = {[Phylogenetic relationships among Asiatic salamanders of the genus Salamandrella based on variability of nuclear genes].}, journal = {Genetika}, volume = {51}, number = {1}, pages = {101-108}, pmid = {25857197}, issn = {0016-6758}, mesh = {Amphibian Proteins/*genetics ; Animals ; Cell Nucleus/genetics ; *Phylogeny ; Salamandridae/*genetics ; Siberia ; Species Specificity ; }, abstract = {Based on sequence variation of three nuclear genome genes (BDNF, POMC, and RAG1), the phylogenetic relationships among Asiatic salamanders of the genus Salamandrella, Siberian salamander (S. keyserlingii) and Schrenk salamander (S. schrenkii), were examined. Both species demonstrated high levels of heterozygosity determined by intraspecific polymorphism. Fixed interspecific differences were revealed at one nucleotide position of the RAG1 gene, and thus the level of interspecific divergence over the three genes constituted only 0.04%. Analysis of the RAG1 polymorphism across the whole range of S. keyserlingii showed that only one gene variant, encoding for modified RAG1 recombinase, had the highest distribution to the north of the Amur region (west and northeast of Siberia). It is possible that the changes in the RAG1 gene in Siberian salamander are of an adaptive nature. However, cases of interspecific hybridization were identified in Jewish autonomous oblast (JAO), which contains one of the range borders between the two Salamandrella species.}, }
@article {pmid25831703, year = {2014}, author = {Musaeva, TS and Berdnikov, AP and Goncharenko, SI and Denisova, EA}, title = {[Efficiency of high-volume hemofiltration in patients with severe sepsis and intracranial hypertension].}, journal = {Anesteziologiia i reanimatologiia}, volume = {59}, number = {6}, pages = {48-51}, pmid = {25831703}, issn = {0201-7563}, mesh = {APACHE ; Carbon Dioxide/blood ; Hemodynamics/physiology ; Hemofiltration/adverse effects/*methods ; Humans ; Intracranial Hypertension/blood/etiology/physiopathology/*therapy ; Intracranial Pressure/physiology ; Oxygen/blood ; Retrospective Studies ; Sepsis/blood/complications/physiopathology/*therapy ; Severity of Illness Index ; Treatment Outcome ; }, abstract = {We performed a retrospective study in 68 patients (144 procedures) with severe sepsis and intracranial hypertension measured by the pressure in the central retinal vein. The patients underwent high-volume hemofiltration (HV-HF) for extrarenal indications. Increased pressure in the central retinal vein was accompanied by critical points of cerebral perfusion pressure and the growth of neurological deficit with inhibition level of consciousness to coma 1. In this case, IHV-HF may be associated with the formation of the critical points of cerebral perfihsion and severe disorders of microcirculation and the lack of resolution of tissue hypoperfusion. In case of intracranial hypertension IHV-HF is not effective in the category of patients where there is a combination ΔpCO2 > 5.9 mm Hg level and GCS <10 points.}, }
@article {pmid25816525, year = {2014}, author = {Karal'nik, BV and Ponomareva, TS and Deriabin, PN and Denisova, TG and Mel'nikova, NN and Tugambaev, TI and Atshabar, BB and Zakarian, SB}, title = {[Effect of immune modulation on immunogenic and protective activity of a live plague vaccine].}, journal = {Zhurnal mikrobiologii, epidemiologii i immunobiologii}, volume = {}, number = {6}, pages = {108-112}, pmid = {25816525}, issn = {0372-9311}, mesh = {Animals ; Antibodies, Bacterial/*biosynthesis ; Antigens, Bacterial/immunology ; Gene Expression ; Guinea Pigs ; Immunity, Cellular/drug effects ; Immunization ; Immunoglobulin G/*biosynthesis ; Immunologic Factors/*administration &amp; dosage/immunology ; Injections, Intravenous ; Interleukin-1beta/*administration &amp; dosage/immunology ; Piperazines/*administration &amp; dosage/immunology ; Plague/blood/immunology/microbiology/*prevention &amp; control ; Plague Vaccine/*administration &amp; dosage/immunology ; Polymers/*administration &amp; dosage ; Rabbits ; Receptors, Antigen, T-Cell/genetics/immunology ; Vaccines, Attenuated ; Yersinia pestis/immunology ; }, abstract = {AIM: Comparative evaluation of the effect of polyoxidonium and betaleukin on immunogenic and protective activity of a live plague vaccine in model animal experiments.<br><br>MATERIALS AND METHODS: Plague vaccine EV, polyoxidonium, betaleukin, erythrocytic antigenic diagnosticum for determination of F1 antibodies and immune reagents for detection of lymphocytes with F1 receptors (LFR) in adhesive test developed by the authors were used. The experiments were carried out in 12 rabbits and 169 guinea pigs.<br><br>RESULTS: Immune modulation accelerated the appearance and disappearance of LFR (early phase) and ensured a more rapid and intensive antibody formation (effector phase). Activation by betaleukin is more pronounced than by polyoxidonium. The more rapid and intensive was the development of early phase, the more effective was antibody response to the vaccine. Immune modulation in the experiment with guinea pigs significantly increased protective activity of the vaccine.<br><br>CONCLUSION: The use of immune modulators increased immunogenic (in both early and effector phases of antigen-specific response) and protective activity of the EV vaccine. A connection between the acceleration of the first phase of antigen-specific response and general intensity of effector phase of immune response to the EV vaccine was detected. ,}, }
@article {pmid25711027, year = {2014}, author = {Maliarchuk, BA and Derenko, MV and Denisova, GA}, title = {[Episodes of adaptive evolution of mitochondrial genome in Asiatic salamanders (Amphibia, Caudata, Hynobiidae)].}, journal = {Genetika}, volume = {50}, number = {2}, pages = {189-196}, pmid = {25711027}, issn = {0016-6758}, mesh = {Adaptation, Biological/genetics ; Amino Acid Sequence ; Animals ; Base Sequence ; *Evolution, Molecular ; *Genetic Speciation ; Genome, Mitochondrial/*genetics ; Phylogeny ; Selection, Genetic/*genetics ; Urodela/genetics ; }, abstract = {To elucidate the effect of natural selection on the evolution of mitochondrial DNA (mtDNA) in Asiatic salamanders of the family Hynobiidae, nucleotide sequences of 12 protein-coding genes were analyzed. Using a mixed effects model of evolution, it was found that, in spite of the pronounced effect of negative selection on the mtDNA evolution in Hynobiidae (which is typical for the animals in general), two phylogenetic clusters, the West Asian one, represented by the genera Ranodon and Paradactylodon, and North Eurasian one, represented by the genus Salamandrella, were formed under the influence of episodic positive selection. Analysis of protein sequences encoded by the mitochondrial genome also supported the influence of positive selection on the evolution of Hynobiidae at some stages of their cladogenesis. It is suggested that the signatures of adaptive evolution detected in the mtDNA of Hynobiidae were determined by the complex and long-lasting history of their formation, accompanied by adaptation to the changing environment.}, }
@article {pmid25588492, year = {2014}, author = {Soldatskiĭ, IuL and Denisova, OA and Ivanenko, AM}, title = {[The comparative effectiveness of framycetin included in combined therapy of adenoiditis in the children].}, journal = {Vestnik otorinolaringologii}, volume = {}, number = {5}, pages = {69-71}, pmid = {25588492}, issn = {0042-4668}, mesh = {Adolescent ; Anti-Infective Agents, Local/administration &amp; dosage/*pharmacology ; Child ; Child, Preschool ; Combined Modality Therapy ; Female ; Framycetin/administration &amp; dosage/*pharmacology ; Humans ; Male ; Nasal Lavage ; Silver Proteins/administration &amp; dosage/*pharmacology ; Tonsillitis/*drug therapy ; Treatment Outcome ; }, abstract = {The objective of the present study was to evaluate the effectiveness of framycetin included in combined therapy of adenoiditis in the children. The study involved 67 children at the mean age of 6.9±2.7 years. Group 1 was comprised of 35 children given framycetin as topical therapy, the patients of group 2 were treated by the endonasal administration of a 2% silver proteinate solution. It was shown that the use of framycetin as a component of combined therapy of adenoiditis enhances the effectiveness of the treatment and compliance to therapy in comparison with the same parameters in the case of the application of traditional topical antibacterial preparations.}, }
@article {pmid25575941, year = {2015}, author = {Rogers, AR and Bohlender, RJ}, title = {Bias in estimators of archaic admixture.}, journal = {Theoretical population biology}, volume = {100C}, number = {}, pages = {63-78}, doi = {10.1016/j.tpb.2014.12.006}, pmid = {25575941}, issn = {1096-0325}, abstract = {This article evaluates bias in one class of methods used to estimate archaic admixture in modern humans. These methods study the pattern of allele sharing among modern and archaic genomes. They are sensitive to "ghost" admixture, which occurs when a population receives archaic DNA from sources not acknowledged by the statistical model. The effect of ghost admixture depends on two factors: branch-length bias and population-size bias. Branch-length bias occurs because a given amount of admixture has a larger effect if the two populations have been separated for a long time. Population-size bias occurs because differences in population size distort branch lengths in the gene genealogy. In the absence of ghost admixture, these effects are small. They become important, however, in the presence of ghost admixture. Estimators differ in the pattern of response. Increasing a given parameter may inflate one estimator but deflate another. For this reason, comparisons among estimators are informative. Using such comparisons, this article supports previous findings that the archaic population was small and that Europeans received little gene flow from archaic populations other than Neanderthals. It also identifies an inconsistency in estimates of archaic admixture into Melanesia.}, }
@article {pmid25563409, year = {2015}, author = {Perry, GH and Kistler, L and Kelaita, MA and Sams, AJ}, title = {Insights into hominin phenotypic and dietary evolution from ancient DNA sequence data.}, journal = {Journal of human evolution}, volume = {79}, number = {}, pages = {55-63}, doi = {10.1016/j.jhevol.2014.10.018}, pmid = {25563409}, issn = {1095-8606}, mesh = {Animals ; Base Sequence ; *Biological Evolution ; DNA/analysis/genetics ; DNA Copy Number Variations ; Feeding Behavior/*physiology ; Fossils ; Genetic Variation ; Genomics ; Hominidae/*genetics/*physiology ; Humans ; Molecular Sequence Data ; Myosin Heavy Chains/genetics ; Neanderthals ; Paleontology ; Phenotype ; Receptors, G-Protein-Coupled/genetics ; Sequence Alignment ; Sequence Analysis, DNA ; }, abstract = {Nuclear genome sequence data from Neandertals, Denisovans, and archaic anatomically modern humans can be used to complement our understanding of hominin evolutionary biology and ecology through i) direct inference of archaic hominin phenotypes, ii) indirect inference of those phenotypes by identifying the effects of previously-introgressed alleles still present among modern humans, or iii) determining the evolutionary timing of relevant hominin-specific genetic changes. Here we review and reanalyze published Neandertal and Denisovan genome sequence data to illustrate an example of the third approach. Specifically, we infer the timing of five human gene presence/absence changes that may be related to particular hominin-specific dietary changes and discuss these results in the context of our broader reconstructions of hominin evolutionary ecology. We show that pseudogenizing (gene loss) mutations in the TAS2R62 and TAS2R64 bitter taste receptor genes and the MYH16 masticatory myosin gene occurred after the hominin-chimpanzee divergence but before the divergence of the human and Neandertal/Denisovan lineages. The absence of a functional MYH16 protein may explain our relatively reduced jaw muscles; this gene loss may have followed the adoption of cooking behavior. In contrast, salivary amylase gene (AMY1) duplications were not observed in the Neandertal and Denisovan genomes, suggesting a relatively recent origin for the AMY1 copy number gains that are observed in modern humans. Thus, if earlier hominins were consuming large quantities of starch-rich underground storage organs, as previously hypothesized, then they were likely doing so without the digestive benefits of increased salivary amylase production. Our most surprising result was the observation of a heterozygous mutation in the first codon of the TAS2R38 bitter taste receptor gene in the Neandertal individual, which likely would have resulted in a non-functional protein and inter-individual PTC (phenylthiocarbamide) taste sensitivity variation, as also observed in both humans and chimpanzees.}, }
@article {pmid25556237, year = {2015}, author = {Lin, YL and Pavlidis, P and Karakoc, E and Ajay, J and Gokcumen, O}, title = {The evolution and functional impact of human deletion variants shared with archaic hominin genomes.}, journal = {Molecular biology and evolution}, volume = {32}, number = {4}, pages = {1008-1019}, pmid = {25556237}, issn = {1537-1719}, mesh = {Alleles ; Animals ; *Evolution, Molecular ; Genetic Variation ; *Genome ; Hominidae/*genetics ; Humans ; *Sequence Deletion ; }, abstract = {Allele sharing between modern and archaic hominin genomes has been variously interpreted to have originated from ancestral genetic structure or through non-African introgression from archaic hominins. However, evolution of polymorphic human deletions that are shared with archaic hominin genomes has yet to be studied. We identified 427 polymorphic human deletions that are shared with archaic hominin genomes, approximately 87% of which originated before the Human-Neandertal divergence (ancient) and only approximately 9% of which have been introgressed from Neandertals (introgressed). Recurrence, incomplete lineage sorting between human and chimp lineages, and hominid-specific insertions constitute the remaining approximately 4% of allele sharing between humans and archaic hominins. We observed that ancient deletions correspond to more than 13% of all common (>5% allele frequency) deletion variation among modern humans. Our analyses indicate that the genomic landscapes of both ancient and introgressed deletion variants were primarily shaped by purifying selection, eliminating large and exonic variants. We found 17 exonic deletions that are shared with archaic hominin genomes, including those leading to three fusion transcripts. The affected genes are involved in metabolism of external and internal compounds, growth and sperm formation, as well as susceptibility to psoriasis and Crohn's disease. Our analyses suggest that these "exonic" deletion variants have evolved through different adaptive forces, including balancing and population-specific positive selection. Our findings reveal that genomic structural variants that are shared between humans and archaic hominin genomes are common among modern humans and can influence biomedically and evolutionarily important phenotypes.}, }
@article {pmid27141645, year = {2015}, author = {Verikovsky, VA and Minakov, OE and Denisova, OI and Bondarenko, EV}, title = {[Results of Investigation of MRSA Susceptibility to Vancomycin in Clinical Units of Large Multifunctional Hospital and Recommendations on Optimization of Antibacterial Therapy of Staphylococcal Infection].}, journal = {Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic]}, volume = {60}, number = {11-12}, pages = {35-38}, pmid = {27141645}, issn = {0235-2990}, mesh = {Anti-Bacterial Agents/administration &amp; dosage/*therapeutic use ; Cities ; Drug Resistance, Bacterial ; Hospital Bed Capacity, 500 and over/standards ; Hospitals, District/standards ; Humans ; Methicillin-Resistant Staphylococcus aureus/*drug effects/isolation &amp; purification ; Microbial Sensitivity Tests/methods ; *Practice Guidelines as Topic ; Russia ; Staphylococcal Infections/*drug therapy/epidemiology/microbiology ; Vancomycin/administration &amp; dosage/*therapeutic use ; }, abstract = {The frequency of MRSA and MRSE isolates in various units of the Voronezh Regional Hospital was investigated by the results of the local microbiological monitoring for 2014. The maximum position of MRSA was recorded in the ICU (38 to 75% of the strains). The MRSA strains were characterized by higher methicillin resistance, mainly from the cardiosurgical units (60 to 91% of the isolates). The use of the E-test for MRSA susceptibility to vancomycin allowed to estimate the validity of the use of various antibiotics active against MRSA in the treatment of inpatients and to reduce the risk of ineffective therapy.}, }
@article {pmid27024918, year = {2015}, author = {Slepowa, OS and Svetlova, EV and Kovaleva, LA and Makarov, PV and Kugusheva, AE and Denisova, EV and Vahova, ES and Zaharova, GY and Kondrat'eva, YA and Andryushin, AE and Demkin, VV}, title = {[PCR study of the human herpes virus type 6 and other viruses of the herpes group in eye diseases].}, journal = {Voprosy virusologii}, volume = {60}, number = {6}, pages = {45-48}, pmid = {27024918}, issn = {0507-4088}, mesh = {Adolescent ; Adult ; Aged ; Aged, 80 and over ; Anterior Chamber/pathology/virology ; Aqueous Humor/virology ; Child ; Child, Preschool ; Cornea/pathology/virology ; Cytomegalovirus/genetics/isolation &amp; purification ; DNA, Viral/*genetics ; Eye Diseases/*diagnosis/pathology/virology ; Female ; Herpesviridae Infections/*diagnosis/pathology/virology ; Herpesvirus 1, Human/genetics/isolation &amp; purification ; Herpesvirus 2, Human/genetics/isolation &amp; purification ; Herpesvirus 4, Human/genetics/isolation &amp; purification ; Herpesvirus 6, Human/genetics/isolation &amp; purification ; Humans ; Male ; Middle Aged ; Polymerase Chain Reaction ; }, abstract = {To study the role of the HHV-6 type in the development of eye diseases PCR tests of blood (152), cornea biopsies (61), and intraocular fluids (11) for HHV-6 and other viruses of the herpes group (HSV type 1 and 2, CMV, EBV) were conducted. It was found that the HHV-6, along with other representatives of the Herpesviridae, can be detected in patients with different clinical forms of ophthalmopathology (174 patients were surveyed). Viral DNA was detected in blood, cornea, and in the anterior chamber fluid. The obtained data allow that the HHV-6 to be suggested as a possible cause of the ophthalmic herpes along with the other viruses of this group. It makes finding the virus DNA an essential step towards setting the etiologic diagnosis of the ophthalmological patients.}, }
@article {pmid25536760, year = {2014}, author = {Denisova, OI and Davydkin, NF and Kulikov, AG}, title = {[The pathogenetic prerequisites for the application of the general magnetic therapy in the children presenting with cerebral ischemia].}, journal = {Voprosy kurortologii, fizioterapii, i lechebnoi fizicheskoi kultury}, volume = {}, number = {5}, pages = {56-60}, pmid = {25536760}, issn = {0042-8787}, mesh = {Brain Ischemia/*etiology/physiopathology/*rehabilitation ; Cerebrovascular Circulation/physiology ; Child ; Humans ; Hypoxia, Brain/prevention &amp; control ; *Magnetic Field Therapy ; Microcirculation/physiology ; }, abstract = {This article presents the analysis of the current literature and the original data of the authors providing the rationale for the use of magnetic therapy for the treatment of the children presenting with cerebral ischemia taking into consideration pathogenesis of this disease. It is demonstrated that the application of the general magnetic field decreases the tone of the cerebral vessels and improves blood flow to the brain which increases resistance to cerebral hypoxia. The results of investigations into the microcirculatory changes and liquor dynamics in conjunction with the ventriculometric measurements give evidence of the effectiveness of the combined treatment of cerebral ischemia making use of general magnetic therapy.}, }
@article {pmid25509854, year = {2013}, author = {Maliarchuk, BA}, title = {[Mutation process in the protein-coding genes of human mitochondrial genome in context of evolution of the genus].}, journal = {Molekuliarnaia biologiia}, volume = {47}, number = {6}, pages = {927-933}, pmid = {25509854}, issn = {0026-8984}, mesh = {Animals ; DNA, Mitochondrial/genetics ; *Evolution, Molecular ; Genome, Human/*genetics ; *Genome, Mitochondrial ; Humans ; Mutation/*genetics ; Neanderthals ; Polymorphism, Genetic ; }, abstract = {The human mitochondrial genome, although it has a small size, is characterized by high level of variation, non-uniformly distributed in groups of nucleotide positions that differ in the degree of variability. Considering the mutation process in human mtDNA relative to the mitochondrial genomes of the genus Homo-neandertals, denisova hominin and other primate species, it appears that more than half (56.5%) variable positions in the human mtDNA protein-coding genes are characterized by back (reverse) mutations to the pre-H. sapiens state of mitochondrial genome. It has been found that hypervariable nucleotide positions show a minimal proportion of specific to H. sapiens mutations, and, conversely, a high proportion of mutations (both nucleotide and amino acid substitutions), leading to the loss of Homo-specific variants of polymorphisms. Most often, polymorphisms specific to H. sapiens arise in result of single forward mutations and disappear mainly due to multiple back mutations, including those in the mutational "hotspots".}, }
@article {pmid25487326, year = {2015}, author = {Sánchez-Quinto, F and Lalueza-Fox, C}, title = {Almost 20 years of Neanderthal palaeogenetics: adaptation, admixture, diversity, demography and extinction.}, journal = {Philosophical transactions of the Royal Society of London. Series B, Biological sciences}, volume = {370}, number = {1660}, pages = {20130374}, pmid = {25487326}, issn = {1471-2970}, mesh = {Adaptation, Biological/*genetics ; Animals ; *Biological Evolution ; DNA, Mitochondrial/genetics ; *Extinction, Biological ; Fossils ; *Genetic Variation ; Genetics, Population ; Genomics/*methods/trends ; *Hybridization, Genetic ; Neanderthals/*genetics ; Population Dynamics ; }, abstract = {Nearly two decades since the first retrieval of Neanderthal DNA, recent advances in next-generation sequencing technologies have allowed the generation of high-coverage genomes from two archaic hominins, a Neanderthal and a Denisovan, as well as a complete mitochondrial genome from remains which probably represent early members of the Neanderthal lineage. This genomic information, coupled with diversity exome data from several Neanderthal specimens is shedding new light on evolutionary processes such as the genetic basis of Neanderthal and modern human-specific adaptations-including morphological and behavioural traits-as well as the extent and nature of the admixture events between them. An emerging picture is that Neanderthals had a long-term small population size, lived in small and isolated groups and probably practised inbreeding at times. Deleterious genetic effects associated with these demographic factors could have played a role in their extinction. The analysis of DNA from further remains making use of new large-scale hybridization-capture-based methods as well as of new approaches to discriminate contaminant DNA sequences will provide genetic information in spatial and temporal scales that could help clarify the Neanderthal's-and our very own-evolutionary history.}, }
@article {pmid25447821, year = {2015}, author = {Adel, S and Kakularam, KR and Horn, T and Reddanna, P and Kuhn, H and Heydeck, D}, title = {Leukotriene signaling in the extinct human subspecies Homo denisovan and Homo neanderthalensis. Structural and functional comparison with Homo sapiens.}, journal = {Archives of biochemistry and biophysics}, volume = {565}, number = {}, pages = {17-24}, doi = {10.1016/j.abb.2014.10.012}, pmid = {25447821}, issn = {1096-0384}, mesh = {Animals ; Databases, Genetic ; Genome, Human/*physiology ; Humans ; Leukotrienes/*genetics/metabolism ; Lipoxygenases/*genetics/metabolism ; Neanderthals/*genetics/metabolism ; Signal Transduction/*genetics ; Species Specificity ; }, abstract = {Mammalian lipoxygenases (LOXs) have been implicated in cell differentiation and in the biosynthesis of pro- and anti-inflammatory lipid mediators. The initial draft sequence of the Homo neanderthalensis genome (coverage of 1.3-fold) suggested defective leukotriene signaling in this archaic human subspecies since expression of essential proteins appeared to be corrupted. Meanwhile high quality genomic sequence data became available for two extinct human subspecies (H. neanderthalensis, Homo denisovan) and completion of the human 1000 genome project provided a comprehensive database characterizing the genetic variability of the human genome. For this study we extracted the nucleotide sequences of selected eicosanoid relevant genes (ALOX5, ALOX15, ALOX12, ALOX15B, ALOX12B, ALOXE3, COX1, COX2, LTA4H, LTC4S, ALOX5AP, CYSLTR1, CYSLTR2, BLTR1, BLTR2) from the corresponding databases. Comparison of the deduced amino acid sequences in connection with site-directed mutagenesis studies and structural modeling suggested that the major enzymes and receptors of leukotriene signaling as well as the two cyclooxygenase isoforms were fully functional in these two extinct human subspecies.}, }
@article {pmid25403398, year = {2014}, author = {Bugayova, LI and Denisova, TD and Morozova, YA and Sergeeva, SA and Kharlamov, IV}, title = {Effect of afobazole administered to pregnant rats during organogenesis on prenatal development of fetuses.}, journal = {Bulletin of experimental biology and medicine}, volume = {158}, number = {1}, pages = {57-60}, doi = {10.1007/s10517-014-2691-x}, pmid = {25403398}, issn = {1573-8221}, mesh = {Animals ; Anti-Anxiety Agents/therapeutic use/*toxicity ; Benzimidazoles/therapeutic use/*toxicity ; Drug Evaluation, Preclinical ; Female ; Fetal Development/*drug effects ; Male ; Maternal-Fetal Exchange ; Morpholines/therapeutic use/*toxicity ; Organogenesis/*drug effects ; Pregnancy ; Pregnancy Complications/*drug therapy ; Rats ; }, abstract = {Experiments on pregnant rats have demonstrated the absence of damaging effect of Afobazole administered during the antenatal period on organogenesis in fetuses. Afobazole in a dose of 5 mg/kg given to pregnant rats on gestation days 6-16 reduced pre- and post-implantation fetal mortality and improved fertility; 20-day-old embryos had no developmental abnormalities and did not differ from controls by craniocaudal size, body weight, and skeleton ossification. Afobazole in a dose of 100 mg/kg reduced pre- and post-implantation fetal mortality, but had no effect on fertility. No congenital malformations were found in the fetuses, but they were characterized by lower craniocaudal size, body weight, and number of ossification foci in the sternum and spine.}, }
@article {pmid25393762, year = {2014}, author = {Lesecque, Y and Glémin, S and Lartillot, N and Mouchiroud, D and Duret, L}, title = {The red queen model of recombination hotspots evolution in the light of archaic and modern human genomes.}, journal = {PLoS genetics}, volume = {10}, number = {11}, pages = {e1004790}, pmid = {25393762}, issn = {1553-7404}, mesh = {Animals ; Chromosomes/genetics ; *Crossing Over, Genetic ; DNA-Binding Proteins ; *Evolution, Molecular ; Gene Conversion ; Genome, Human ; Histone-Lysine N-Methyltransferase/*genetics ; Humans ; Meiosis/genetics ; Pan troglodytes ; *Recombination, Genetic ; }, abstract = {Recombination is an essential process in eukaryotes, which increases diversity by disrupting genetic linkage between loci and ensures the proper segregation of chromosomes during meiosis. In the human genome, recombination events are clustered in hotspots, whose location is determined by the PRDM9 protein. There is evidence that the location of hotspots evolves rapidly, as a consequence of changes in PRDM9 DNA-binding domain. However, the reasons for these changes and the rate at which they occur are not known. In this study, we investigated the evolution of human hotspot loci and of PRDM9 target motifs, both in modern and archaic human lineages (Denisovan) to quantify the dynamic of hotspot turnover during the recent period of human evolution. We show that present-day human hotspots are young: they have been active only during the last 10% of the time since the divergence from chimpanzee, starting to be operating shortly before the split between Denisovans and modern humans. Surprisingly, however, our analyses indicate that Denisovan recombination hotspots did not overlap with modern human ones, despite sharing similar PRDM9 target motifs. We further show that high-affinity PRDM9 target motifs are subject to a strong self-destructive drive, known as biased gene conversion (BGC), which should lead to the loss of the majority of them in the next 3 MYR. This depletion of PRDM9 genomic targets is expected to decrease fitness, and thereby to favor new PRDM9 alleles binding different motifs. Our refined estimates of the age and life expectancy of human hotspots provide empirical evidence in support of the Red Queen hypothesis of recombination hotspots evolution.}, }
@article {pmid25301575, year = {2014}, author = {Derenko, M and Malyarchuk, B and Denisova, G and Perkova, M and Litvinov, A and Grzybowski, T and Dambueva, I and Skonieczna, K and Rogalla, U and Tsybovsky, I and Zakharov, I}, title = {Western Eurasian ancestry in modern Siberians based on mitogenomic data.}, journal = {BMC evolutionary biology}, volume = {14}, number = {}, pages = {217}, pmid = {25301575}, issn = {1471-2148}, mesh = {Asian Continental Ancestry Group/genetics ; DNA, Mitochondrial/*genetics ; European Continental Ancestry Group/genetics ; Female ; *Gene Pool ; Genetics, Medical ; *Genetics, Population ; Humans ; Molecular Sequence Data ; Phylogeny ; Phylogeography ; Siberia ; }, abstract = {BACKGROUND: Although the genetic heritage of aboriginal Siberians is mostly of eastern Asian ancestry, a substantial western Eurasian component is observed in the majority of northern Asian populations. Traces of at least two migrations into southern Siberia, one from eastern Europe and the other from western Asia/the Caucasus have been detected previously in mitochondrial gene pools of modern Siberians.<br><br>RESULTS: We report here 166 new complete mitochondrial DNA (mtDNA) sequences that allow us to expand and re-analyze the available data sets of western Eurasian lineages found in northern Asian populations, define the phylogenetic status of Siberian-specific subclades and search for links between mtDNA haplotypes/subclades and events of human migrations. From a survey of 158 western Eurasian mtDNA genomes found in Siberia we estimate that nearly 40% of them most likely have western Asian and another 29% European ancestry. It is striking that 65 of northern Asian mitogenomes, i.e. ~41%, fall into 19 branches and subclades which can be considered as Siberian-specific being found so far only in Siberian populations. From the coalescence analysis it is evident that the sequence divergence of Siberian-specific subclades was relatively small, corresponding to only 0.6-9.5 kya (using the complete mtDNA rate) and 1-6 kya (coding region rate).<br><br>CONCLUSIONS: The phylogeographic analysis implies that the western Eurasian founders, giving rise to Siberian specific subclades, may trace their ancestry only to the early and mid-Holocene, though some of genetic lineages may trace their ancestry back to the end of Last Glacial Maximum (LGM). We have not found the modern northern Asians to have western Eurasian genetic components of sufficient antiquity to indicate traces of pre-LGM expansions.}, }
@article {pmid25277105, year = {2014}, author = {Schneider, E and El Hajj, N and Haaf, T}, title = {Epigenetic information from ancient DNA provides new insights into human evolution. Commentary on Gokhman D et al. (2014): Reconstructing the DNA methylation maps of the Neanderthal and the Denisovan. Science 344:523-527.}, journal = {Brain, behavior and evolution}, volume = {84}, number = {3}, pages = {169-171}, doi = {10.1159/000365650}, pmid = {25277105}, issn = {1421-9743}, mesh = {Animals ; *DNA Methylation ; *Epigenesis, Genetic ; *Evolution, Molecular ; *Genome, Human ; Humans ; Neanderthals/*genetics ; }, }
@article {pmid25269182, year = {2014}, author = {Cherniaevskaia, GM and Maksimenko, GV and Beloborodova, ÉI and Ustiuzhanina, EA and Denisova, OA}, title = {[Clinical and morphological peculiarities of the clinical course of duodenal ulcer in patients with bronchial asthma].}, journal = {Klinicheskaia meditsina}, volume = {92}, number = {2}, pages = {47-52}, pmid = {25269182}, issn = {0023-2149}, mesh = {Adolescent ; Adult ; Asthma/epidemiology/*pathology ; Comorbidity ; Duodenal Ulcer/epidemiology/etiology/*pathology ; Gastric Mucosa/microbiology/*pathology/physiopathology ; Helicobacter Infections/*complications/epidemiology/pathology ; Helicobacter pylori/*pathogenicity ; Humans ; Inflammation/epidemiology/microbiology/pathology ; Middle Aged ; Prospective Studies ; Young Adult ; }, abstract = {This work was aimed to study clinical features of H. pylori-associated duodenal ulcer (DU) and elucidate morphological features of gastric mucosa (GM) in patients with bronchial asthma (BA). Simultaneous prospective examination of 118 patients aged 18-64 yr included clinical and endoscopic study of the gastroduodenal region with the assessment of gastroenterological symptoms and morphological analysis of GM and duodenal biopsies by histological, histochemical, and morphometric methods. It was shown that GM inflammation in patients with DU and BA is associated not only with H. pylori infection but also with the phase of BA. Structural changes of GM in the patients with DU and BA, unlike those with DU without BA, were apparent not only in the antrum but also in the fundus. Growing density of eosinophil, neutrophil and mast cell infiltration of fundal and antral GM as well as increased total number of cells in the antrum reflects active immune-mediated inflammation in GM lamina propria. It is concluded that negative effect of uncontrolled BA on the clinical course of DU is not restricted to the association of DU exacerbation with the absence of BA control; it is also responsible for enhanced activity of gastritis. One of the possible factors determining combination of H. pylori-associated duodenal ulcer with BA is chronic hyperergic inflammation and marked structural changes in GM.}, }
@article {pmid25265662, year = {2014}, author = {Denisova, OA and Cherniavskaia, GM and Beloborodova, ÉI and Topol'nitskiĭ, EB and Iakimenko, IuV and Chernogoriuk, GÉ and Beloborodova, EV and Strezh, IuA and Vil'danova, LR}, title = {[A case of thoracic actinomycosis].}, journal = {Klinicheskaia meditsina}, volume = {92}, number = {1}, pages = {59-61}, pmid = {25265662}, issn = {0023-2149}, mesh = {Actinomyces/*pathogenicity ; Actinomycosis/*diagnosis/drug therapy/pathology ; Amoxicillin-Potassium Clavulanate Combination/administration &amp; dosage/pharmacology ; Anti-Bacterial Agents/administration &amp; dosage/*pharmacology ; Humans ; Male ; Middle Aged ; Pneumonia, Bacterial/*diagnosis/drug therapy/pathology ; Respiratory Tract Infections/diagnosis/drug therapy/pathology ; Sulbactam/administration &amp; dosage/pharmacology ; Treatment Outcome ; }, abstract = {A case of thoracic actinomycosis manifest as round shadow in the lung is described. Diagnosis was based on the presence of actinomycetes in a transthoracic lung biopsy sample. Treatment for 3 months resulted in recovery. No relapse was documented during 1 year follow-up period.}, }
@article {pmid25257410, year = {2014}, author = {Pinchuk, TV and Fedulaev, YN and Khairetdinova, GA and Denisova, NN and Chura, OV and Logunova, IY}, title = {Anti-inflammatory effects of simvastatin in patients with chronic heart failure.}, journal = {Bulletin of experimental biology and medicine}, volume = {157}, number = {5}, pages = {552-554}, doi = {10.1007/s10517-014-2612-z}, pmid = {25257410}, issn = {1573-8221}, mesh = {Aged ; Anti-Inflammatory Agents/*therapeutic use ; Chronic Disease ; Female ; Heart Failure/*drug therapy ; Humans ; Inflammation Mediators/blood ; Male ; Middle Aged ; Simvastatin/*therapeutic use ; }, abstract = {Proinflammatory markers were evaluated in patients with chronic heart failure of ischemic origin and essential hypertension with preserved left-ventricular ejection fraction before and after a 6-month course of simvastatin therapy (20 mg/day). The study was carried out in 125 patients with diastolic dysfunction manifested as impaired relaxation and pseudonormalization. The main group received standard therapy for chronic heart failure and simvastatin, controls received only standard therapy. In addition, the results in the main group were compared in patients with different types of left-ventricular diastolic dysfunction. Simvastatin therapy significantly reduced the levels of C-reactive protein and IL-6.}, }
@article {pmid25249249, year = {2015}, author = {Kang, L and Michalak, P}, title = {The evolution of cancer-related genes in hominoids.}, journal = {Journal of molecular evolution}, volume = {80}, number = {1}, pages = {37-41}, pmid = {25249249}, issn = {1432-1432}, mesh = {Animals ; Base Sequence ; *Evolution, Molecular ; *Genes, Tumor Suppressor ; Hominidae/*genetics ; Humans ; *Oncogenes ; Sequence Homology, Nucleic Acid ; }, abstract = {The evolution of cancer suppression is essential for the maintenance of multicellularity. The lack of correlation between body size and cancer risk across species, known as Peto's paradox, suggests that genetic variation in cancer resistance is sufficient to compensate for increases of cell numbers in bigger animals. To assess evolutionary dynamics of cancer-related genes, we analyzed Ka, Ks,and Ka/Ks values in 120 oncogenes and tumor suppressor genes (TSG) among seven hominoid species, including two extinct species, Neanderthal and Denisovan. Ka/Ks of tumor suppressor genes tended to be higher relative to that of oncogenes, consistent with relaxed purifying selection acting on the former. Ka/Ks values were positively correlated with TSG scores, but negatively correlated with oncogene scores, suggesting opposing selection pressures operating on the two groups of cancer-related genes. Additionally, we found 108 species-divergent substitutions that were prevalent germline genotypes in some species but in humans appeared only as somatic cancerous mutations. Better understanding the resistance to cancer may lead to new methods of cancer prevention in humans.}, }
@article {pmid25172957, year = {2014}, author = {Racimo, F and Kuhlwilm, M and Slatkin, M}, title = {A test for ancient selective sweeps and an application to candidate sites in modern humans.}, journal = {Molecular biology and evolution}, volume = {31}, number = {12}, pages = {3344-3358}, pmid = {25172957}, issn = {1537-1719}, support = {R01 GM040282/GM/NIGMS NIH HHS/United States ; R01-GM40282/GM/NIGMS NIH HHS/United States ; }, mesh = {Animals ; Bayes Theorem ; *Evolution, Molecular ; Genome, Human ; Humans ; *Models, Genetic ; Neanderthals/genetics ; Polymorphism, Single Nucleotide ; Selection, Genetic ; }, abstract = {We introduce a new method to detect ancient selective sweeps centered on a candidate site. We explored different patterns produced by sweeps around a fixed beneficial mutation, and found that a particularly informative statistic measures the consistency between majority haplotypes near the mutation and genotypic data from a closely related population. We incorporated this statistic into an approximate Bayesian computation (ABC) method that tests for sweeps at a candidate site. We applied this method to simulated data and show that it has some power to detect sweeps that occurred more than 10,000 generations in the past. We also applied it to 1,000 Genomes and Complete Genomics data combined with high-coverage Denisovan and Neanderthal genomes to test for sweeps in modern humans since the separation from the Neanderthal-Denisovan ancestor. We tested sites at which humans are fixed for the derived (i.e., nonchimpanzee allele) whereas the Neanderthal and Denisovan genomes are homozygous for the ancestral allele. We observe only weak differences in statistics indicative of selection between functional categories. When we compare patterns of scaled diversity or use our ABC approach, we fail to find a significant difference in signals of classic selective sweeps between regions surrounding nonsynonymous and synonymous changes, but we detect a slight enrichment for reduced scaled diversity around splice site changes. We also present a list of candidate sites that show high probability of having undergone a classic sweep in the modern human lineage since the split from Neanderthals and Denisovans.}, }
@article {pmid25142605, year = {2014}, author = {Lee, A and Huntley, D and Aiewsakun, P and Kanda, RK and Lynn, C and Tristem, M}, title = {Novel Denisovan and Neanderthal retroviruses.}, journal = {Journal of virology}, volume = {88}, number = {21}, pages = {12907-12909}, pmid = {25142605}, issn = {1098-5514}, support = {//Wellcome Trust/United Kingdom ; }, mesh = {Animals ; Endogenous Retroviruses/classification/*genetics/*isolation &amp; purification ; Female ; Fossils/*virology ; Genome ; Hominidae/*virology ; }, abstract = {Following the recent availability of high-coverage genomes for Denisovan and Neanderthal hominids, we conducted a screen for endogenized retroviruses, identifying six novel, previously unreported HERV-K(HML2) elements (HERV-K is human endogenous retrovirus K). These elements are absent from the human genome (hg38) and appear to be unique to archaic hominids. These findings provide further evidence supporting the recent activity of the HERV-K(HML2) group, which has been implicated in human disease. They will also provide insights into the evolution of archaic hominids.}, }
@article {pmid25110108, year = {2014}, author = {Malyarchuk, B and Derenko, M and Denisova, G}, title = {A mitogenomic phylogeny and genetic history of sable (Martes zibellina).}, journal = {Gene}, volume = {550}, number = {1}, pages = {56-67}, doi = {10.1016/j.gene.2014.08.015}, pmid = {25110108}, issn = {1879-0038}, mesh = {Animals ; Asia ; Bayes Theorem ; Cytochromes b/genetics ; DNA, Mitochondrial/chemistry/classification/*genetics ; Europe ; Evolution, Molecular ; Genetic Variation ; Genetics, Population ; Genome, Mitochondrial/*genetics ; Geography ; Molecular Sequence Data ; Mustelidae/*genetics ; NADH Dehydrogenase/genetics ; *Phylogeny ; Selection, Genetic ; Sequence Analysis, DNA ; Time Factors ; }, abstract = {We assessed phylogeny of sable (Martes zibellina, Linnaeus, 1758) by sequence analysis of nearly complete, new mitochondrial genomes in 36 specimens from different localities in northern Eurasia (Primorye, Khabarovsk and Krasnoyarsk regions, the Kamchatka Peninsula, the Kuril Islands and the Urals). Phylogenetic analysis of mtDNA sequences demonstrates that two clades, A and BC, radiated about 200-300 thousandyears ago (kya) according to results of Bayesian molecular clock and RelTime analyses of different mitogenome alignments (nearly complete mtDNA sequences, protein-coding region, and synonymous sites), while the age estimates of clades A, B and C fall within the Late Pleistocene (~50-140 kya). Bayesian skyline plots (BSPs) of sable population size change based on analysis of nearly complete mtDNAs show an expansion around 40 kya in the warm Karganian time, without a decline of population size around the Last Glacial Maximum (21 kya). The BSPs based on synonymous clock rate indicate that M. zibellina experienced demographic expansions later, approximately 22 kya. The A2a clade that colonized Kamchatka ~23-50 kya (depending on the mutation rate used) survived the last glaciation there as demonstrated by the BSP analysis. In addition, we have found evidence of positive selection acting at ND4 and cytochrome b genes, thereby suggesting adaptive evolution of the A2a clade in Kamchatka.}, }
@article {pmid25073341, year = {2014}, author = {Petrov, AG and Denisova, SV and Odintseva, OV and Raudina, SN}, title = {[Methodologic approaches to farmacologic economic analysis of actual inpatient treatment of occupational diseases].}, journal = {Meditsina truda i promyshlennaia ekologiia}, volume = {}, number = {2}, pages = {42-45}, pmid = {25073341}, issn = {1026-9428}, mesh = {Hearing Loss, Sensorineural/drug therapy/economics ; *Hospitalization/economics/statistics &amp; numerical data ; Humans ; Models, Statistical ; *Occupational Diseases/drug therapy/economics ; }, abstract = {To improve miners' health nowadays, effective application of available means and implication of economic management methods are necessary, as quality medical and pharmaceutic care for miners requires significant financial, material and working efforts both from the government and from every institution operating in health care.}, }
@article {pmid25051667, year = {2014}, author = {Katamanova, EV and Shevchenko, OI and Lakhman, OL and Denisova, IA}, title = {[Cognitive disorders in patients with chronic mercury intoxication].}, journal = {Meditsina truda i promyshlennaia ekologiia}, volume = {}, number = {4}, pages = {7-12}, pmid = {25051667}, issn = {1026-9428}, mesh = {Cognition Disorders/classification/etiology/*physiopathology ; Humans ; Male ; Mercury Poisoning, Nervous System/complications/*physiopathology ; Middle Aged ; Occupational Diseases/chemically induced/*physiopathology ; Occupational Exposure/*adverse effects ; Severity of Illness Index ; }, abstract = {To assess severity of cognitive disorders in chronic mercury intoxication, the authors performed claster and discrimination analysis of neuropsychologic and neurophysiologic research data from workers exposed to mercury during long length of service, from patients with early and marked stages of chronic mercurial intoxication. Cognitive disorders in chronic mercurial intoxication have three severity degrees, in the light degree disorders patients demonstrate lower amplitude of cognitive evoked potentials, poor long-term memory and associative thinking. Moderate cognitive disorders are characterized by decreased visual, long-term memory, concentration of attention, poor optic and spatial gnosis. Marked cognitive disorders with chronic mercurial intoxication present with more decreased long-term, short-term, picturesque memory, poor intellect, optic and spatial gnosis and associative thinking.}, }
@article {pmid25043523, year = {2014}, author = {Jorner, K and Emanuelsson, R and Dahlstrand, C and Tong, H and Denisova, AV and Ottosson, H}, title = {Impact of ground- and excited-state aromaticity on cyclopentadiene and silole excitation energies and excited-state polarities.}, journal = {Chemistry (Weinheim an der Bergstrasse, Germany)}, volume = {20}, number = {30}, pages = {9295-9303}, doi = {10.1002/chem.201402577}, pmid = {25043523}, issn = {1521-3765}, abstract = {A new qualitative model for estimating the properties of substituted cyclopentadienes and siloles in their lowest ππ* excited states is introduced and confirmed through quantum chemical calculations, and then applied to explain earlier reported experimental excitation energies. According to our model, which is based on excited-state aromaticity and antiaromaticity, siloles and cyclopentadienes are cross-hyperconjugated "aromatic chameleons" that adapt their electronic structures to conform to the various aromaticity rules in different electronic states (Hückel's rule in the π(2) electronic ground state (S0) and Baird's rule in the lowest ππ* excited singlet and triplet states (S1 and T1)). By using pen-and-paper arguments, one can explain polarity changes upon excitation of substituted cyclopentadienes and siloles, and one can tune their lowest excitation energies by combined considerations of ground- and excited-state aromaticity/antiaromaticity effects. Finally, the "aromatic chameleon" model can be extended to other monocyclic compound classes of potential use in organic electronics, thereby providing a unified view of the S0, T1, and S1 states of a range of different cyclic cross-π-conjugated and cross-hyperconjugated compound classes.}, }
@article {pmid25043035, year = {2014}, author = {Huerta-Sánchez, E and Jin, X and Asan,  and Bianba, Z and Peter, BM and Vinckenbosch, N and Liang, Y and Yi, X and He, M and Somel, M and Ni, P and Wang, B and Ou, X and Huasang,  and Luosang, J and Cuo, ZX and Li, K and Gao, G and Yin, Y and Wang, W and Zhang, X and Xu, X and Yang, H and Li, Y and Wang, J and Wang, J and Nielsen, R}, title = {Altitude adaptation in Tibetans caused by introgression of Denisovan-like DNA.}, journal = {Nature}, volume = {512}, number = {7513}, pages = {194-197}, pmid = {25043035}, issn = {1476-4687}, support = {R01 HG003229/HG/NHGRI NIH HHS/United States ; R01HG003229/HG/NHGRI NIH HHS/United States ; R01HG003229-08S2/HG/NHGRI NIH HHS/United States ; }, mesh = {Adaptation, Physiological/*genetics ; *Altitude ; Animals ; Asian Continental Ancestry Group/genetics ; Basic Helix-Loop-Helix Transcription Factors/genetics ; DNA/*genetics ; Gene Frequency ; *Genetic Variation ; Haplotypes ; Hominidae/*genetics ; Humans ; Polymorphism, Single Nucleotide ; Tibet ; }, abstract = {As modern humans migrated out of Africa, they encountered many new environmental conditions, including greater temperature extremes, different pathogens and higher altitudes. These diverse environments are likely to have acted as agents of natural selection and to have led to local adaptations. One of the most celebrated examples in humans is the adaptation of Tibetans to the hypoxic environment of the high-altitude Tibetan plateau. A hypoxia pathway gene, EPAS1, was previously identified as having the most extreme signature of positive selection in Tibetans, and was shown to be associated with differences in haemoglobin concentration at high altitude. Re-sequencing the region around EPAS1 in 40 Tibetan and 40 Han individuals, we find that this gene has a highly unusual haplotype structure that can only be convincingly explained by introgression of DNA from Denisovan or Denisovan-related individuals into humans. Scanning a larger set of worldwide populations, we find that the selected haplotype is only found in Denisovans and in Tibetans, and at very low frequency among Han Chinese. Furthermore, the length of the haplotype, and the fact that it is not found in any other populations, makes it unlikely that the haplotype sharing between Tibetans and Denisovans was caused by incomplete ancestral lineage sorting rather than introgression. Our findings illustrate that admixture with other hominin species has provided genetic variation that helped humans to adapt to new environments.}, }
@article {pmid25001002, year = {2014}, author = {Schwartz, JJ and Roach, DJ and Thomas, JH and Shendure, J}, title = {Primate evolution of the recombination regulator PRDM9.}, journal = {Nature communications}, volume = {5}, number = {}, pages = {4370}, pmid = {25001002}, issn = {2041-1723}, support = {R01 HG006283/HG/NHGRI NIH HHS/United States ; HG006283/HG/NHGRI NIH HHS/United States ; //Howard Hughes Medical Institute/United States ; }, mesh = {Amino Acid Sequence ; Animals ; Base Sequence ; *Evolution, Molecular ; Genetic Variation ; Histone-Lysine N-Methyltransferase/*genetics ; Molecular Sequence Data ; Primates/*genetics ; Selection, Genetic ; Sequence Analysis, DNA ; Zinc Fingers ; }, abstract = {The PRDM9 gene encodes a protein with a highly variable tandem-repeat zinc finger (ZF) DNA-binding domain that plays a key role in determining sequence-specific hotspots of meiotic recombination genome wide. Here we survey the diversity of the PRDM9 ZF domain by sequencing this region in 64 primates from 18 species, revealing 68 unique alleles across all groups. We report ubiquitous positive selection at nucleotide positions corresponding to DNA contact residues and the expansion of ZFs within clades, which confirms the rapid evolution of the ZF domain throughout the primate lineage. Alignment of Neandertal and Denisovan sequences suggests that PRDM9 in archaic hominins was closely related to present-day human alleles that are rare and specific to African populations. In the context of its role in reproduction, our results are consistent with variation in PRDM9 contributing to speciation events in primates.}, }
@article {pmid24845746, year = {2014}, author = {Denisova, K and Kibbe, MM and Cholewiak, SA and Kim, SH}, title = {Intra- and intermanual curvature aftereffect can be obtained via tool-touch.}, journal = {IEEE transactions on haptics}, volume = {7}, number = {1}, pages = {61-66}, doi = {10.1109/TOH.2013.63}, pmid = {24845746}, issn = {2329-4051}, mesh = {Adult ; Feedback, Sensory/*physiology ; Female ; Humans ; Male ; Space Perception/*physiology ; Touch Perception/*physiology ; }, abstract = {We examined the perception of virtual curved surfaces explored with a tool. We found a reliable curvature aftereffect, suggesting neural representation of the curvature in the absence of direct touch. Intermanual transfer of the aftereffect suggests that this representation is somewhat independent of the hand used to explore the surface.}, }
@article {pmid24832686, year = {2014}, author = {Wong, LP and Lai, JK and Saw, WY and Ong, RT and Cheng, AY and Pillai, NE and Liu, X and Xu, W and Chen, P and Foo, JN and Tan, LW and Koo, SH and Soong, R and Wenk, MR and Lim, WY and Khor, CC and Little, P and Chia, KS and Teo, YY}, title = {Insights into the genetic structure and diversity of 38 South Asian Indians from deep whole-genome sequencing.}, journal = {PLoS genetics}, volume = {10}, number = {5}, pages = {e1004377}, pmid = {24832686}, issn = {1553-7404}, mesh = {*Genetic Variation ; *Genetics, Population ; *Genome, Human ; Haplotypes ; Humans ; India ; Polymorphism, Single Nucleotide ; }, abstract = {South Asia possesses a significant amount of genetic diversity due to considerable intergroup differences in culture and language. There have been numerous reports on the genetic structure of Asian Indians, although these have mostly relied on genotyping microarrays or targeted sequencing of the mitochondria and Y chromosomes. Asian Indians in Singapore are primarily descendants of immigrants from Dravidian-language-speaking states in south India, and 38 individuals from the general population underwent deep whole-genome sequencing with a target coverage of 30X as part of the Singapore Sequencing Indian Project (SSIP). The genetic structure and diversity of these samples were compared against samples from the Singapore Sequencing Malay Project and populations in Phase 1 of the 1,000 Genomes Project (1 KGP). SSIP samples exhibited greater intra-population genetic diversity and possessed higher heterozygous-to-homozygous genotype ratio than other Asian populations. When compared against a panel of well-defined Asian Indians, the genetic makeup of the SSIP samples was closely related to South Indians. However, even though the SSIP samples clustered distinctly from the Europeans in the global population structure analysis with autosomal SNPs, eight samples were assigned to mitochondrial haplogroups that were predominantly present in Europeans and possessed higher European admixture than the remaining samples. An analysis of the relative relatedness between SSIP with two archaic hominins (Denisovan, Neanderthal) identified higher ancient admixture in East Asian populations than in SSIP. The data resource for these samples is publicly available and is expected to serve as a valuable complement to the South Asian samples in Phase 3 of 1 KGP.}, }
@article {pmid24786081, year = {2014}, author = {Gokhman, D and Lavi, E and Prüfer, K and Fraga, MF and Riancho, JA and Kelso, J and Pääbo, S and Meshorer, E and Carmel, L}, title = {Reconstructing the DNA methylation maps of the Neandertal and the Denisovan.}, journal = {Science (New York, N.Y.)}, volume = {344}, number = {6183}, pages = {523-527}, doi = {10.1126/science.1250368}, pmid = {24786081}, issn = {1095-9203}, mesh = {Animals ; *DNA Methylation ; *Epigenesis, Genetic ; *Evolution, Molecular ; *Genome, Human ; Humans ; Neanderthals/*genetics ; }, abstract = {Ancient DNA sequencing has recently provided high-coverage archaic human genomes. However, the evolution of epigenetic regulation along the human lineage remains largely unexplored. We reconstructed the full DNA methylation maps of the Neandertal and the Denisovan by harnessing the natural degradation processes of methylated and unmethylated cytosines. Comparing these ancient methylation maps to those of present-day humans, we identified ~2000 differentially methylated regions (DMRs). Particularly, we found substantial methylation changes in the HOXD cluster that may explain anatomical differences between archaic and present-day humans. Additionally, we found that DMRs are significantly more likely to be associated with diseases. This study provides insight into the epigenetic landscape of our closest evolutionary relatives and opens a window to explore the epigenomes of extinct species.}, }
@article {pmid24752266, year = {2014}, author = {Denisova, OV and Söderholm, S and Virtanen, S and Von Schantz, C and Bychkov, D and Vashchinkina, E and Desloovere, J and Tynell, J and Ikonen, N and Theisen, LL and Nyman, TA and Matikainen, S and Kallioniemi, O and Julkunen, I and Muller, CP and Saelens, X and Verkhusha, VV and Kainov, DE}, title = {Akt inhibitor MK2206 prevents influenza pH1N1 virus infection in vitro.}, journal = {Antimicrobial agents and chemotherapy}, volume = {58}, number = {7}, pages = {3689-3696}, pmid = {24752266}, issn = {1098-6596}, mesh = {Cell Line ; Cytokines/metabolism ; Heterocyclic Compounds, 3-Ring/*pharmacology ; Host-Pathogen Interactions/drug effects ; Humans ; In Vitro Techniques ; *Influenza A Virus, H1N1 Subtype ; Influenza, Human/*prevention &amp; control/virology ; Molecular Sequence Data ; Oncogene Protein v-akt/*antagonists &amp; inhibitors ; Phosphoproteins/metabolism ; Protease Inhibitors/*pharmacology ; RNA, Small Interfering/genetics ; Transfection ; Viral Plaque Assay ; }, abstract = {The influenza pH1N1 virus caused a global flu pandemic in 2009 and continues manifestation as a seasonal virus. Better understanding of the virus-host cell interaction could result in development of better prevention and treatment options. Here we show that the Akt inhibitor MK2206 blocks influenza pH1N1 virus infection in vitro. In particular, at noncytotoxic concentrations, MK2206 alters Akt signaling and inhibits endocytic uptake of the virus. Interestingly, MK2206 is unable to inhibit H3N2, H7N9, and H5N1 viruses, indicating that pH1N1 evolved specific requirements for efficient infection. Thus, Akt signaling could be exploited further for development of better therapeutics against pH1N1 virus.}, }
@article {pmid24667833, year = {2014}, author = {Sazzini, M and Schiavo, G and De Fanti, S and Martelli, PL and Casadio, R and Luiselli, D}, title = {Searching for signatures of cold adaptations in modern and archaic humans: hints from the brown adipose tissue genes.}, journal = {Heredity}, volume = {113}, number = {3}, pages = {259-267}, pmid = {24667833}, issn = {1365-2540}, mesh = {Adaptation, Physiological/*genetics ; Adipose Tissue, Brown/*metabolism ; Alleles ; Biological Evolution ; Climate ; Cold Temperature ; Fossils ; Genome/*genetics ; Humans/*genetics ; Thermogenesis/*genetics ; }, abstract = {Adaptation to low temperatures has been reasonably developed in the human species during the colonization of the Eurasian landmass subsequent to Out of Africa migrations of anatomically modern humans. In addition to morphological and cultural changes, also metabolic ones are supposed to have favored human isolation from cold and body heat production and this can be hypothesized also for most Neandertal and at least for some Denisovan populations, which lived in geographical areas that strongly experienced the last glacial period. Modulation of non-shivering thermogenesis, for which adipocytes belonging to the brown adipose tissue are the most specialized cells, might have driven these metabolic adaptations. To perform an exploratory analysis aimed at looking into this hypothesis, variation at 28 genes involved in such functional pathway was investigated in modern populations from different climate zones, as well as in Neandertal and Denisovan genomes. Patterns of variation at the LEPR gene, strongly related to increased heat dissipation by mitochondria, appeared to have been shaped by positive selection in modern East Asians, but not in Europeans. Moreover, a single potentially cold-adapted LEPR allele, different from the supposed adaptive one identified in Homo sapiens, was found also in Neandertal and Denisovan genomes. These findings suggest that independent mechanisms for cold adaptations might have been developed in different non-African human groups, as well as that the evolution of possible enhanced thermal efficiency in Neandertals and in some Denisovan populations has plausibly entailed significant changes also in other functional pathways than in the examined one.}, }
@article {pmid24599118, year = {2014}, author = {Mariotti, M and Smith, TF and Sudmant, PH and Goldberger, G}, title = {Pseudogenization of testis-specific Lfg5 predates human/Neanderthal divergence.}, journal = {Journal of human genetics}, volume = {59}, number = {5}, pages = {288-291}, pmid = {24599118}, issn = {1435-232X}, mesh = {Animals ; *Evolution, Molecular ; Exons ; Genomics ; Humans ; Introns ; Male ; Multigene Family ; Mutation ; Neanderthals/*genetics ; Organ Specificity/*genetics ; Phylogeny ; Testis/*metabolism ; }, abstract = {Recent reviews discussed the critical roles of apoptosis in human spermatogenesis and infertility. These reviews highlight the FasL-induced caspase cascade in apoptosis lending importance to our discovery of the pseudogene status of the Lfg5 gene in modern humans, Neanderthal and the Denisovan. This gene is a member of the ancient and highly conserved apoptosis Lifeguard family. This pseudogenization is the result of a premature stop codon at the 3'-end of exon 8 not found in any other ortholog. With the current exception of the domesticated bovine and buffalo, Lfg5's expression in mammals is testis-specific. A full analysis of this gene, its phylogenetic context and its recent hominin changes suggest its inactivation was likely under selection in human evolution.}, }
@article {pmid24501955, year = {2013}, author = {Agafonova, EV and Malanicheva, TG and Denisova, SN}, title = {[Subpopulations and phagocytic activity of monocytes in chronic gastroduodenitis in children].}, journal = {Eksperimental'naia i klinicheskaia gastroenterologiia = Experimental &amp; clinical gastroenterology}, volume = {}, number = {5}, pages = {108-111}, pmid = {24501955}, issn = {1682-8658}, mesh = {Adolescent ; Candida/*immunology ; Candidiasis/*immunology ; Child ; Child, Preschool ; Duodenitis/*immunology ; Female ; Gastritis/*immunology ; Helicobacter Infections ; Helicobacter pylori/*immunology ; Humans ; Male ; Monocytes/*immunology ; Phagocytosis/*immunology ; }, abstract = {There was conducted a study of the phagocytic activity, immunophenotype and peripheral blood monocytes by flow cytometry in children with chronic gastroduodenitis associated with Helicobacter pylori, as well as the association of Helicobacter pylori with fungi of the genus Candida and markers of secondary immune deficiency. The differential changes in the structure of circulating profile of monocytes were revealed, that indicate the pathogenetic significance of these disorders in chronic gastroduodenitis with H. pylori etiology, as well as at association of Helicobacter pylori with fungi of the genus Candida. Violations of the phagocytic activity of monocytes in chronic gastroduodenitis in children are associated with depression of different stages of phagocytosis--capture functions, mobilization, killing, intracellular biocidity. A severe depression in phagocytic activity of monocytes occurs in CGD associated with Hp and fungi of the genus Candida.}, }
@article {pmid24450152, year = {2013}, author = {Malyarchuk, BA and Denisova, GA and Derenko, MV}, title = {[Peculiarities of phosphoglycerate kinase-1 pseudogene evolution in Schrenck salamander (Salamandrella schrenckii Strauch, 1870)].}, journal = {Genetika}, volume = {49}, number = {7}, pages = {830-837}, doi = {10.7868/s0016675813070102}, pmid = {24450152}, issn = {0016-6758}, mesh = {Amino Acid Sequence ; Animals ; *Evolution, Molecular ; Molecular Sequence Data ; Phosphoglycerate Kinase/*genetics ; Phylogeny ; Polymorphism, Single Nucleotide ; Pseudogenes/*genetics ; Urodela/*genetics ; }, abstract = {Processed copies of genes generally evolve in neutral mode as pseudogenes, however, some of them might be important sources of new functional genes. The psiPGK1 pseudogene has been discovered in Schrenck salamander (Salamandrella schrenckii, Amphibia, Caudata, Hynobiidae) via polymerase chain reaction used to amplify the phosphoglycerate kinase 1 gene (PGK1). This pseudogene is an intronless copy of PGK1 gene absent of exon 6. Analysis of psiPGK1 pseudogene polymorphism has demonstrated that it lacks mutations, which results in shifts in the stop codons and reading frames, as well as that the interspecies variation of this pseudogene was inconsistent with the neutral model of evolution. In addition, the pattern of phylogeographic differentiation of the psiPGK1 variants mainly coincides with that observed in mitochondrial DNA. These observations allow it to be suggested that the psiPGK1 pseudogene is a new functional gene in the Schrenck salamander.}, }
@article {pmid24392153, year = {2014}, author = {Hughes, GM and Teeling, EC and Higgins, DG}, title = {Loss of olfactory receptor function in hominin evolution.}, journal = {PloS one}, volume = {9}, number = {1}, pages = {e84714}, pmid = {24392153}, issn = {1932-6203}, mesh = {Amino Acid Sequence ; Animals ; *Biological Evolution ; Databases, Nucleic Acid ; Evolution, Molecular ; Genome ; Genomics ; Hominidae/*genetics ; Humans ; Molecular Sequence Data ; Multigene Family ; Olfactory Receptor Neurons/*metabolism ; Phylogeny ; Receptors, Odorant/chemistry/classification/*genetics ; Sequence Alignment ; }, abstract = {The mammalian sense of smell is governed by the largest gene family, which encodes the olfactory receptors (ORs). The gain and loss of OR genes is typically correlated with adaptations to various ecological niches. Modern humans have 853 OR genes but 55% of these have lost their function. Here we show evidence of additional OR loss of function in the Neanderthal and Denisovan hominin genomes using comparative genomic methodologies. Ten Neanderthal and 8 Denisovan ORs show evidence of loss of function that differ from the reference modern human OR genome. Some of these losses are also present in a subset of modern humans, while some are unique to each lineage. Morphological changes in the cranium of Neanderthals suggest different sensory arrangements to that of modern humans. We identify differences in functional olfactory receptor genes among modern humans, Neanderthals and Denisovans, suggesting varied loss of function across all three taxa and we highlight the utility of using genomic information to elucidate the sensory niches of extinct species.}, }
@article {pmid25929022, year = {2014}, author = {Pogozheva, AV and Sorokina, EY and Baturin, AK and Peskova, EV and Makurina, ON and Levin, LG and Soto, SKh and Aristarkhova, TA and Korosteleva, MM and Denisova, NN and Solntseva, TN and Aleshina, IV and Toboleva, MA and Redzyuk, LA and Polyakova, AV}, title = {[The role of the Consultative and Diagnostic Centre "Healthy Nutrition" in the diagnosis and nutritional prevention of non-communicable diseases].}, journal = {Voprosy pitaniia}, volume = {83}, number = {6}, pages = {52-57}, pmid = {25929022}, issn = {0042-8833}, mesh = {Body Mass Index ; Diabetes Mellitus, Type 2/blood/*diagnosis/genetics/*prevention &amp; control ; Energy Intake ; Female ; Humans ; Male ; *Nutritional Status ; Obesity/blood/*diagnosis/genetics/*prevention &amp; control ; Polymorphism, Genetic ; }, abstract = {In a consultative and diagnostic center "Healthy Nutrition" of Institute of Nutrition the nutritional status of 3500 patients (mean age 48.4 ± 0.3 years) liv- ing in the Moscow region, using a system Nutritest IP-3, including genomic analysis has been examined. In the analysis of dietary intake by an average review, increased energy intake due to excess intake of the total (44.2% energy) and saturated fat (13.6%) has been shown. 30.0% of patients were overweight and 34.1% were obese. Osteopenia was detected in 31.0% of men and 25.0% women, osteoporosis--20.9% and 30.3%, respectively. Analysis of the results of biochemical studies revealed increased cholesterol in 68.7% of patients, LDL cholesterol--at 63.9%, triglycerides-- at 22.5%, glucose--at 29.4%. The frequency of the occurrence of risk alleles of genes associated with the development of obesity and type 2 diabetes mellitus was: 47.8%--for the polymorphism rs9939609 (FTO gene), 8.3%--for polymorphism rs4994 (gene ADRB3), 60.2%--for the polymorphism rs659366 (gene UCP2), 36.6%--for the rs5219 polymorphism in the gene of ATP-dependent potassium channel.}, }
@article {pmid25845144, year = {2014}, author = {Isaeva, NA and Torubarov, FS and Denisova, EA and Zvereva, ZF and Koronotova, MA}, title = {[Evaluating psychophysiologic adaptation state in operators of Bilibino nuclear power station].}, journal = {Meditsina truda i promyshlennaia ekologiia}, volume = {}, number = {11}, pages = {16-20}, pmid = {25845144}, issn = {1026-9428}, mesh = {Adaptation, Physiological/*physiology ; Adaptation, Psychological/*physiology ; Adult ; Humans ; Middle Aged ; Neuropsychological Tests ; *Nuclear Power Plants ; Occupational Diseases/epidemiology/*physiopathology/psychology ; Occupational Exposure/analysis ; Psychophysiologic Disorders/epidemiology/*physiopathology/psychology ; Russia ; }, abstract = {The study revealed that 60% operators of Bilibino nuclear power station suffer from psychosomatic diseases, 41.7% of them are assigned to occupational group of workers, and major part of the examinees with psychosomatic diseases (45.82%) are aged 41-50, high integral level ofpsychophysiologic adaptation is revealed in 5 examinees (12.5%), medium integral level--in 12 examinees (30%). Lower integral level of psychophysiologic adaptation manifested in decrease in psychophysiologic and physiologic levels.}, }
@article {pmid25816630, year = {2014}, author = {Shostak, NA and Muradiants, AA and Kondrashov, AA and Denisova, SN}, title = {[Clinical efficacy instant goat milk in the complex therapy and prevention of osteoporosis in patients with rheumatoid arthritis].}, journal = {Voprosy pitaniia}, volume = {83}, number = {5}, pages = {79-85}, pmid = {25816630}, issn = {0042-8833}, mesh = {Animals ; Arthritis, Rheumatoid/*blood/*prevention &amp; control ; Calcifediol/blood ; Calcitriol/blood ; Calcium/blood ; Female ; *Goats ; Humans ; Male ; Middle Aged ; *Milk ; Osteoporosis, Postmenopausal/*blood/*prevention &amp; control ; Phosphorus/blood ; }, abstract = {Osteoporosis (OP) in rheumatoid arthritis (RA) refers to a secondary immune-mediated metabolic osteopathy characterized by periarticular and systemic decreased bone mass, impaired bone strength and increased risk of fractures. According to some studies, adding milk in the diet helps to increase bone mineral density and to reduce the risk of osteoporosis and maintain normal levels of vitamin D. To study the state of mineral and bone metabolism in RA patients zeith osteopenic syndrome and to evaluate the effectiveness of treatment and prevention of OP by adding dry goat milk "Amalteya" in the diet. The study included 42 patients with a documented diagnosis of RA (ACR, 1987) - 23 men (mean age 59 years) and 19 postmenopausal women (mean age 62 years) with the presence of osteoporosis and osteopenia according to the dual-energy X-ray absorptiometry. 21 (50%) RA patients (main group) received standard antiosteoporotichesky (alendronate 70 mg/week + calcium 1000 mg/day + Vitamin D3 800 IU/day) therapy and milk powder Amalteya® (400 ml/day). The control group (21 patients with RA) received only standard antiosteoporotic therapy. Follow-up lasted for 6 months. The concentration of total calcium in the blood of RA patients was on average 2.33 mmol/l, ionized Ca - 1,18 mmol/l and inorganic P - 1,09 mmol/l, which corresponds to normal values. Vitamin D deficiency was found in 17,5% of patients, and failure - in 32,5% of patients with RA. After 6 months of the treatment it was found that b-CrossLaps levels tend to be reducing in both of the groups and with reduction of bone formation marker osteocalcin in the group not receiving goat milk. Also, due to the background of ongoing combinative therapy it was clear that concentrations of 1,25(OH)2D and 25(OH)D in the blood serum are increasing (by 18,5-28,2% at the main group and by 8,0-17,9% at the control group), however, inter-group differences was below the level of the reliable importance. It was strongly marked in the group who received goat's milk "Amalteya®". Reduced levels of vitamin D in the blood is typical for 50% of RA patients with osteopenic syndrome with normal values of calcium-phosphorus metabolism. Combination therapy and prevention of osteoporosis in patients with RA with an additional inclusion in the diet of the daily administration of 400 ml of goat's milk Amalthea® has a positive impact on bone metabolism.}, }
@article {pmid25799827, year = {2014}, author = {Vizel', IIu and Shmelev, EI and Baranova, OP and Barlamov, PN and Borodina, GL and Denisova, OA and Dobin, VL and Kulbaisov, AM and Kupaev, VI and Listopadova, MV and Ovsiannikov, NV and Os'kin, DN and Petrov, DV and Solov'ev, KI and Shul'zhenko, LV and Vizel', AA}, title = {[Multicenter retrospective analysis of the patients with sarcoidosis with a 10 year interval in observations].}, journal = {Klinicheskaia meditsina}, volume = {92}, number = {6}, pages = {28-34}, pmid = {25799827}, issn = {0023-2149}, mesh = {Adult ; Antitubercular Agents/*therapeutic use ; Disease-Free Survival ; Female ; Glucocorticoids/*therapeutic use ; Humans ; Lung/diagnostic imaging ; Male ; Patient Acuity ; Prognosis ; Radiography ; Recurrence ; Respiratory Function Tests/methods ; Risk Factors ; Russia/epidemiology ; *Sarcoidosis/diagnosis/epidemiology/physiopathology/therapy ; }, abstract = {Comparison of the state of 83 patients with histologically confirmed sarcoidosis observed with a 10 year interval revealed remission in 47% of the cases. The main factors having negative effect on prognosis of the disease included extrapulmonary symptoms, the use ofcorticosteroids (at all stages, especially at stage I and in Lofgren syndrome) and antituberculosis drugs, positive TB test. Risk factors of relapses were stage II sarcoidosis, the use of systemic corticosteroids in patients with Lofgren syndrome and antituberculosis drugs, initially low FEV1/FVLC ratio and the number of lymphocytes in peripheral blood.}, }
@article {pmid25734313, year = {2014}, author = {Mazur, EM and Soldatskiĭ, IL and Ivanenko, AM and Denisova, OA and Severin, TV}, title = {[Foreign bodies (disk batteries) in the nose].}, journal = {Vestnik otorinolaringologii}, volume = {}, number = {6}, pages = {64-65}, doi = {10.17116/otorino2014664-65}, pmid = {25734313}, issn = {0042-4668}, mesh = {Child, Preschool ; Electric Power Supplies/*adverse effects ; Female ; *Foreign Bodies ; Humans ; Male ; Nose/*injuries ; }, abstract = {This paper reports the results of analysis of the treatment of 8 children after the removal a disk battery from the nasal cavity. It was shown that the restoration of all the structures of the nasal cavity is possible if the foreign body remains in it during a short (up to 5 hours) time. The longer presence of such a body in the nasal cavity gives rise to post-traumatic defects, in the first place septal perforations and injuries to the inferior turbinated bone. In such cases, the foreign body must be immediately removed from the nasal cavity, and the child should be placed under thorough medical observation taking into consideration the long process of rejection of necrotic tissues and healing of the resulting defects.}, }
@article {pmid24367647, year = {2013}, author = {Reno, PL and McLean, CY and Hines, JE and Capellini, TD and Bejerano, G and Kingsley, DM}, title = {A penile spine/vibrissa enhancer sequence is missing in modern and extinct humans but is retained in multiple primates with penile spines and sensory vibrissae.}, journal = {PloS one}, volume = {8}, number = {12}, pages = {e84258}, pmid = {24367647}, issn = {1932-6203}, support = {RR016483/RR/NCRR NIH HHS/United States ; RR014491/RR/NCRR NIH HHS/United States ; U42 RR015087/RR/NCRR NIH HHS/United States ; C06 RR014491/RR/NCRR NIH HHS/United States ; P50 HG002568/HG/NHGRI NIH HHS/United States ; 5P50HG2568/HG/NHGRI NIH HHS/United States ; RR015087/RR/NCRR NIH HHS/United States ; //Howard Hughes Medical Institute/United States ; C06 RR016483/RR/NCRR NIH HHS/United States ; }, mesh = {Animals ; Enhancer Elements, Genetic/*genetics ; *Extinction, Biological ; Genome, Human/genetics ; Humans ; Male ; Molecular Sequence Data ; Penis/*metabolism ; Primates/*genetics ; Receptors, Androgen/*genetics ; Sequence Analysis ; Somatosensory Cortex/physiology ; Species Specificity ; Vibrissae/*metabolism/physiology ; }, abstract = {Previous studies show that humans have a large genomic deletion downstream of the Androgen Receptor gene that eliminates an ancestral mammalian regulatory enhancer that drives expression in developing penile spines and sensory vibrissae. Here we use a combination of large-scale sequence analysis and PCR amplification to demonstrate that the penile spine/vibrissa enhancer is missing in all humans surveyed and in the Neandertal and Denisovan genomes, but is present in DNA samples of chimpanzees and bonobos, as well as in multiple other great apes and primates that maintain some form of penile integumentary appendage and facial vibrissae. These results further strengthen the association between the presence of the penile spine/vibrissa enhancer and the presence of penile spines and macro- or micro- vibrissae in non-human primates as well as show that loss of the enhancer is both a distinctive and characteristic feature of the human lineage.}, }
@article {pmid24343584, year = {2014}, author = {Baklanova, YV and Denisova, TA and Maksimova, LG and Tyutyunnik, AP and Baklanova, IV and Shein, IR and Neder, RB and Tarakina, NV}, title = {Synthesis and characterisation of new MO(OH)2 (M = Zr, Hf) oxyhydroxides and related Li2MO3 salts.}, journal = {Dalton transactions (Cambridge, England : 2003)}, volume = {43}, number = {7}, pages = {2755-2763}, doi = {10.1039/c3dt52929k}, pmid = {24343584}, issn = {1477-9234}, abstract = {Two new solid MO(OH)2 (M = Zr, Hf) oxyhydroxides have been synthesised by an ion-exchange reaction from Li2MO3 (M = Zr, Hf) precursors obtained by a citrate combustion technique. The crystal structure of the oxyhydroxides has been solved by direct methods and refined using Rietveld full profile fitting based on X-ray powder diffraction data. Both oxyhydroxides crystallize in a P2(1)/c monoclinic unit cell and have a structure resembling that of the related salts. Detailed characterisation of the fine-structure features and chemical bonding in precursors and oxyhydroxide powders has been performed using vibrational spectroscopy, nuclear magnetic resonance spectroscopy, scanning electron microscopy, pair distribution function analysis and quantum-chemical modelling.}, }
@article {pmid24336922, year = {2014}, author = {Ding, Q and Hu, Y and Xu, S and Wang, J and Jin, L}, title = {Neanderthal introgression at chromosome 3p21.31 was under positive natural selection in East Asians.}, journal = {Molecular biology and evolution}, volume = {31}, number = {3}, pages = {683-695}, doi = {10.1093/molbev/mst260}, pmid = {24336922}, issn = {1537-1719}, mesh = {Adaptation, Physiological/genetics ; Alleles ; Animals ; Asian Continental Ancestry Group/*genetics ; Chromosomes, Human, Pair 3/*genetics ; Far East ; Genetic Variation ; Geography ; Haplotypes/genetics ; Humans ; Linkage Disequilibrium/genetics ; Models, Genetic ; Neanderthals/*genetics ; Pan troglodytes/genetics ; Phylogeny ; Polymorphism, Single Nucleotide/genetics ; Recombination, Genetic/genetics ; *Selection, Genetic ; Time Factors ; }, abstract = {Studies of the Neanderthal and Denisovan genomes demonstrate archaic hominin introgression in Eurasians. Here, we present evidence of Neanderthal introgression within the chromosome 3p21.31 region, occurring with a high frequency in East Asians (ranging from 49.4% to 66.5%) and at a low frequency in Europeans. We also detected a signal of strong positive selection in this region only in East Asians. Our data indicate that likely candidate targets of selection include rs12488302-T and its associated alleles--among which four are nonsynonymous, including rs35455589-G in HYAL2, a gene related to the cellular response to ultraviolet-B irradiation. Furthermore, suggestive evidence supports latitude-dependent selection, implicating a role of ultraviolet-B. Interestingly, the distribution of rs35455589-G suggests that this allele was lost during the exodus of ancestors of modern Eurasians from Africa and reintroduced to Eurasians from Neanderthals.}, }
@article {pmid24262833, year = {2013}, author = {Marchi, E and Kanapin, A and Byott, M and Magiorkinis, G and Belshaw, R}, title = {Neanderthal and Denisovan retroviruses in modern humans.}, journal = {Current biology : CB}, volume = {23}, number = {22}, pages = {R994-R995}, pmid = {24262833}, issn = {1879-0445}, support = {MR/K010565/1//Medical Research Council/United Kingdom ; //Wellcome Trust/United Kingdom ; }, mesh = {Animals ; Humans ; Neanderthals/*genetics ; Retroviridae/*genetics ; }, }
@article {pmid24244704, year = {2013}, author = {Derenko, M and Malyarchuk, B and Bahmanimehr, A and Denisova, G and Perkova, M and Farjadian, S and Yepiskoposyan, L}, title = {Complete mitochondrial DNA diversity in Iranians.}, journal = {PloS one}, volume = {8}, number = {11}, pages = {e80673}, pmid = {24244704}, issn = {1932-6203}, mesh = {DNA, Mitochondrial/*genetics ; European Continental Ancestry Group ; Gene Flow/genetics ; Genetics, Population ; Haplotypes ; Humans ; Iran ; Phylogeny ; }, abstract = {Due to its pivotal geographical location and proximity to transcontinental migratory routes, Iran has played a key role in subsequent migrations, both prehistoric and historic, between Africa, Asia and Europe. To shed light on the genetic structure of the Iranian population as well as on the expansion patterns and population movements which affected this region, the complete mitochondrial genomes of 352 Iranians were obtained. All Iranian populations studied here exhibit similarly high diversity values comparable to the other groups from the Caucasus, Anatolia and Europe. The results of AMOVA and MDS analyses did not associate any regional and/or linguistic group of populations in the Anatolia/Caucasus and Iran region pointing to close genetic positions of Persians and Qashqais to each other and to Armenians, and Azeris from Iran to Georgians. By reconstructing the complete mtDNA phylogeny of haplogroups R2, N3, U1, U3, U5a1g, U7, H13, HV2, HV12, M5a and C5c we have found a previously unexplored genetic connection between the studied Iranian populations and the Arabian Peninsula, India, Near East and Europe, likely the result of both ancient and recent gene flow. Our results for Persians and Qashqais point to a continuous increase of the population sizes from ∼24 kya to the present, although the phase between 14-24 kya is thought to be hyperarid according to the Gulf Oasis model. Since this would have affected hunter-gatherer ranges and mobility patterns and forced them to increasingly rely on coastal resources, this transition can explain the human expansion across the Persian Gulf region.}, }
@article {pmid24174545, year = {2013}, author = {Hochreiter, S}, title = {HapFABIA: identification of very short segments of identity by descent characterized by rare variants in large sequencing data.}, journal = {Nucleic acids research}, volume = {41}, number = {22}, pages = {e202}, pmid = {24174545}, issn = {1362-4962}, mesh = {*Genetic Variation ; Genomics ; Genotyping Techniques ; High-Throughput Nucleotide Sequencing/*methods ; Humans ; Inheritance Patterns ; Sequence Analysis, DNA/*methods ; }, abstract = {Identity by descent (IBD) can be reliably detected for long shared DNA segments, which are found in related individuals. However, many studies contain cohorts of unrelated individuals that share only short IBD segments. New sequencing technologies facilitate identification of short IBD segments through rare variants, which convey more information on IBD than common variants. Current IBD detection methods, however, are not designed to use rare variants for the detection of short IBD segments. Short IBD segments reveal genetic structures at high resolution. Therefore, they can help to improve imputation and phasing, to increase genotyping accuracy for low-coverage sequencing and to increase the power of association studies. Since short IBD segments are further assumed to be old, they can shed light on the evolutionary history of humans. We propose HapFABIA, a computational method that applies biclustering to identify very short IBD segments characterized by rare variants. HapFABIA is designed to detect short IBD segments in genotype data that were obtained from next-generation sequencing, but can also be applied to DNA microarray data. Especially in next-generation sequencing data, HapFABIA exploits rare variants for IBD detection. HapFABIA significantly outperformed competing algorithms at detecting short IBD segments on artificial and simulated data with rare variants. HapFABIA identified 160 588 different short IBD segments characterized by rare variants with a median length of 23 kb (mean 24 kb) in data for chromosome 1 of the 1000 Genomes Project. These short IBD segments contain 752 000 single nucleotide variants (SNVs), which account for 39% of the rare variants and 23.5% of all variants. The vast majority-152 000 IBD segments-are shared by Africans, while only 19 000 and 11 000 are shared by Europeans and Asians, respectively. IBD segments that match the Denisova or the Neandertal genome are found significantly more often in Asians and Europeans but also, in some cases exclusively, in Africans. The lengths of IBD segments and their sharing between continental populations indicate that many short IBD segments from chromosome 1 existed before humans migrated out of Africa. Thus, rare variants that tag these short IBD segments predate human migration from Africa. The software package HapFABIA is available from Bioconductor. All data sets, result files and programs for data simulation, preprocessing and evaluation are supplied at http://www.bioinf.jku.at/research/short-IBD.}, }
@article {pmid24132124, year = {2013}, author = {Malyarchuk, B and Derenko, M and Denisova, G and Khoyt, S and Woźniak, M and Grzybowski, T and Zakharov, I}, title = {Y-chromosome diversity in the Kalmyks at the ethnical and tribal levels.}, journal = {Journal of human genetics}, volume = {58}, number = {12}, pages = {804-811}, doi = {10.1038/jhg.2013.108}, pmid = {24132124}, issn = {1435-232X}, mesh = {Asian Continental Ancestry Group/*genetics ; Chromosomes, Human, Y/*genetics ; Ethnic Groups/*genetics ; European Continental Ancestry Group/*genetics ; Gene Flow/genetics ; Gene Frequency/genetics ; Genetics, Population ; Haplotypes ; Humans ; Male ; Microsatellite Repeats/genetics ; Polymorphism, Genetic/*genetics ; }, abstract = {The Mongolic-speaking Kalmyks currently inhabiting the steppes of the Volga region have Central Asian ancestry and are organized into the tribal groups. The genetic relationships among these tribes and their origin have remained obscure. We analyzed 17 short tandem repeat and 44 binary polymorphisms of Y-chromosome in 426 individuals mainly from three major tribes of the Kalmyks (the Torguuds, Dörwöds and Khoshuuds). Among these tribes, the Dörwöds and Torguuds, as well as the Kalmyks collectively as an ethnic group, showed relatively close genetic affinities to each other and to the Mongols and Altaian Kazakhs, whereas the Khoshuuds were clearly separated from all of them, gathering with the Manchu, Tibetans or Evenks (depending on the algorithm used to calculate genetic distances). The genetic results also indicate that paternal gene flow from East Europeans to the Kalmyks is very little, despite their cohabitation in the North Caspian Steppe during the last 380 years. The occurrence of unique cluster of N1c-Tat haplotypes in the Khoshuuds, which dates to about 340 years and is likely to have East European ancestry, is considered as a result of interethnic contacts occurred soon after the appearance of the Kalmyk tribes in the Volga-Ural region.}, }
@article {pmid24064255, year = {2014}, author = {Malyarchuk, B and Derenko, M and Mikhailova, E and Denisova, G}, title = {Phylogenetic relationships among Neoechinorhynchus species (Acanthocephala: Neoechinorhynchidae) from North-East Asia based on molecular data.}, journal = {Parasitology international}, volume = {63}, number = {1}, pages = {100-107}, doi = {10.1016/j.parint.2013.09.012}, pmid = {24064255}, issn = {1873-0329}, mesh = {Acanthocephala/*genetics ; Animals ; Asia/epidemiology ; DNA/genetics ; Demography ; Fish Diseases/epidemiology/parasitology ; Fishes ; *Genetic Variation ; Helminthiasis, Animal/epidemiology/parasitology ; *Phylogeny ; Species Specificity ; }, abstract = {Phylogenetic and statistical analyses of DNA sequences of two genes, cytochrome oxidase subunit 1 (cox 1) of the mitochondrial DNA and 18S subunit of the nuclear ribosomal RNA (18S rRNA), was used to characterize Neoechinorhynchus species from fishes collected in different localities of North-East Asia. It has been found that four species can be clearly recognized using molecular markers-Neoechinorhynchus tumidus, Neoechinorhynchus beringianus, Neoechinorhynchus simansularis and Neoechinorhynchus salmonis. 18S sequences ascribed to Neoechinorhynchus crassus specimens from North-East Asia were identical to those of N. tumidus, but differed substantially from North American N. crassus. We renamed North-East Asian N. crassus specimens to N. sp., although the possibility that they represent a subspecies of N. tumidus cannot be excluded, taking into account a relatively small distance between cox 1 sequences of North-East Asian specimens of N. crassus and N. tumidus. Maximum likelihood, maximum parsimony and Bayesian inference analyses were performed for phylogeny reconstruction. All the phylogenetic trees showed that North-East Asian species of Neoechinorhynchus analyzed in this study represent independent clades, with the only exception of N. tumidus and N. sp. for 18S data. Phylogenetic analysis has shown that the majority of species sampled (N. tumidus+N. sp., N. simansularis and N. beringianus) are probably very closely related, while N. salmonis occupies separate position in the trees, possibly indicating a North American origin of this species.}, }
@article {pmid23980394, year = {2013}, author = {Ovchinnikov, IV}, title = {Hominin evolution and gene flow in the Pleistocene Africa.}, journal = {Anthropologischer Anzeiger; Bericht uber die biologisch-anthropologische Literatur}, volume = {70}, number = {2}, pages = {221-227}, doi = {10.1127/0003-5548/2013/0313}, pmid = {23980394}, issn = {0003-5548}, mesh = {Africa ; Animals ; Anthropology, Physical ; DNA, Mitochondrial/genetics ; *Evolution, Molecular ; Fossils ; *Gene Flow ; Genome, Mitochondrial/genetics ; Hominidae/*genetics ; Humans ; Phylogeny ; }, abstract = {Africa demonstrates a complex process of the hominin evolution with a series of adaptive radiations during several millions of years that led to diverse morphological forms. Recently, Hammer et al. (2011) and Harvati et al. (2011) provided integrated morphological and genetic evidence of interbreeding between modern humans and unknown archaic hominins in Africa as recently as 35,000 years ago. However, a genetic evidence of hybridization between hominin lineages during the Lower and Middle Pleistocene epochs is unknown and the direct retrieval of DNA from extinct lineages of African hominins remains elusive. The availability of both nuclear and mitochondrial genome sequences from modern humans, Neanderthals, and Denisovans allows collecting nuclear DNA sequences of mitochondrial origin (numts) inserted into the nuclear genome of the ancestral hominin lineages and drawing conclusions about the hominin evolution in the remote past. The mtDNA and numt analysis uncovered a deep division of mtDNA lineages that existed in African hominins in the Middle Pleistocene. The first cluster included the human and Neanderthal-like mtDNA sequences while the second consisted of DNA sequences that are known today as mtAncestor-1, a nuclear fossil of the mtDNA, and the Denisova mtDNA isolated from a bone and a tooth found in southern Siberia. The two groups initially diverged 610,000-1,110,000 years ago. Approximately 220,000 years after the primary split, the Denisova - mtAncestor-1 mtDNA lineages mixed with the mtDNA pool of an ancestral population of Neanderthals and modern humans. This admixture after the profound division is demonstrated by the transposition of the Denisova-like mtDNA sequence into the nuclear genome of an ancestor of Neanderthals and modern humans. This finding suggests the matrilineal genetic structure among the Middle Pleistocene hominins as well as the existence of gene flow between African hominin lineages. Through paleogenomic analyses, it is impossible to exclude the theory that population structure and gene flow in African hominins influenced the admixture pattern observed in the nuclear genomes of non-Africans.}, }
@article {pmid23959642, year = {2012}, author = {Setó-Salvia, N and Sánchez-Quinto, F and Carbonell, E and Lorenzo, C and Comas, D and Clarimón, J}, title = {Using the neanderthal and denisova genetic data to understand the common MAPT 17q21 inversion in modern humans.}, journal = {Human biology}, volume = {84}, number = {6}, pages = {633-640}, doi = {10.3378/027.084.0605}, pmid = {23959642}, issn = {1534-6617}, mesh = {Alleles ; Animals ; Biological Evolution ; *Chromosome Inversion ; Genome, Human ; Haplotypes ; Hominidae/*genetics ; Humans ; Linkage Disequilibrium ; Neanderthals/genetics ; Polymorphism, Genetic ; tau Proteins/*genetics ; }, abstract = {The polymorphic inversion on 17q21, that includes the MAPT gene, represents a unique locus in the human genome characterized by a large region with strong linkage disequilibrium. Two distinct haplotypes, H1 and H2, exist in modern humans, and H1 has been unequivocally related to several neurodegenerative disorders. Recent data indicate that recurrent inversions of this genomic region have occurred through primate evolution, with the H2 haplotype being the ancestral state. Neandertals harbored the H1 haplotype; however, until now, no data were available for the Denisova hominin. Neandertals and Denisovans are sister groups that share a common ancestor with modern humans. We analyzed the MAPT sequence and assessed the differences between modern humans, Neandertals, Denisovans, and great apes. Our analysis indicated that the Denisova hominin carried the H1 haplotype, and the Neandertal and Denisova common ancestor probably shared the same subhaplotype (H1j). We also found 68 intronic variants within the MAPT gene, 23 exclusive to Denisova hominin, 6 limited to Neandertals, and 24 exclusive to present-day humans. Our results reinforce previous data; this suggests that the 17q21 inversion arose within the modern human lineage. The data also indicate that archaic hominins that coexisted in Eurasia probably shared the same MAPT subhaplotype, and this can be found in almost 2% of chromosomes from European ancestry.}, }
@article {pmid23951901, year = {2013}, author = {Agafonova, EV and Malanicheva, TG and Denisova, SN}, title = {[Defects of neutrophil function in chronic gastroduodenitis in children].}, journal = {Eksperimental'naia i klinicheskaia gastroenterologiia = Experimental &amp; clinical gastroenterology}, volume = {}, number = {1}, pages = {60-63}, pmid = {23951901}, issn = {1682-8658}, mesh = {Adolescent ; Candida albicans/isolation &amp; purification ; Candidiasis/*blood/complications/microbiology/pathology ; Case-Control Studies ; Child ; Chronic Disease ; Data Interpretation, Statistical ; Duodenitis/*blood/complications/microbiology/pathology ; Gastritis/*blood/complications/microbiology/pathology ; Helicobacter Infections/*blood/complications/microbiology/pathology ; Helicobacter pylori/isolation &amp; purification ; Humans ; Neutrophils/immunology/*physiology ; Phagocytosis/physiology ; }, abstract = {At present, chronic gastroduodenitis (CGD) occupies one ofthe leading places in the structure of diseases of the gastrointestinal tract in children. In the etiology of CGD, along with the leading pathogenic Helicobacterpylori (HP), the role of the fungal flora increased. The aim of the work was to evaluate the functional activity of neutrophils in children with the CGD, associated with HP and Candida albicans. Among 110 children in the age from 7 to 17 years with chronic gastroduodenitis, associated with Helicobacter pylory(HP), as well as the association of HP with Candida albicans and the markers of secondary immune insufficiency, a study of the phagocytic activity and immune phenotype of neutrophils by flow cytofluorimetry was conducted. Differentiated peculiarities of the phagocytic activity of neutrophils in association with bacterial pathogens (HP) and fungal flora were identified. The transformation of the immune phenotype was combined with a significant depression of the phagocytic and microbicidal functions, more pronounced with the association of HP and Candida albicans. Circulating mannano protein antigen of Candida albicans influenced on the surface of phenotype of neutrophils, increasing the expression of protopathic and HLADR-receptors, and decreasing the expression of adhesion receptors and cytolysis. Thus, in case of chronic gastroduodenitis in children, there was a considerable transformation of the phenotype of neutrophil with differentiated characteristics at the association with bacterial (HP) pathogens and fungal flora. The obtained data should be taken into account when carrying out medical activities, and the doctors should include in the composition of complex therapy of CGD, associated with Candida albicans, drugs, aimed at immunocorrection of the identified violations}, }
@article {pmid23906376, year = {2013}, author = {Hedrick, PW}, title = {Adaptive introgression in animals: examples and comparison to new mutation and standing variation as sources of adaptive variation.}, journal = {Molecular ecology}, volume = {22}, number = {18}, pages = {4606-4618}, doi = {10.1111/mec.12415}, pmid = {23906376}, issn = {1365-294X}, mesh = {Adaptation, Biological/*genetics ; Animals ; *Evolution, Molecular ; Genetic Variation ; Humans ; Models, Genetic ; Mutation ; *Selection, Genetic ; }, abstract = {Adaptive genetic variation has been thought to originate primarily from either new mutation or standing variation. Another potential source of adaptive variation is adaptive variants from other (donor) species that are introgressed into the (recipient) species, termed adaptive introgression. Here, the various attributes of these three potential sources of adaptive variation are compared. For example, the rate of adaptive change is generally thought to be faster from standing variation, slower from mutation and potentially intermediate from adaptive introgression. Additionally, the higher initial frequency of adaptive variation from standing variation and lower initial frequency from mutation might result in a higher probability of fixation of the adaptive variants for standing variation. Adaptive variation from introgression might have higher initial frequency than new adaptive mutations but lower than that from standing variation, again making the impact of adaptive introgression variation potentially intermediate. Adaptive introgressive variants might have multiple changes within a gene and affect multiple loci, an advantage also potentially found for adaptive standing variation but not for new adaptive mutants. The processes that might produce a common variant in two taxa, convergence, trans-species polymorphism from incomplete lineage sorting or from balancing selection and adaptive introgression, are also compared. Finally, potential examples of adaptive introgression in animals, including balancing selection for multiple alleles for major histocompatibility complex (MHC), S and csd genes, pesticide resistance in mice, black colour in wolves and white colour in coyotes, Neanderthal or Denisovan ancestry in humans, mimicry genes in Heliconius butterflies, beak traits in Darwin's finches, yellow skin in chickens and non-native ancestry in an endangered native salamander, are examined.}, }
@article {pmid23889734, year = {2013}, author = {Bimbo, LM and Denisova, OV and Mäkilä, E and Kaasalainen, M and De Brabander, JK and Hirvonen, J and Salonen, J and Kakkola, L and Kainov, D and Santos, HA}, title = {Inhibition of influenza A virus infection in vitro by saliphenylhalamide-loaded porous silicon nanoparticles.}, journal = {ACS nano}, volume = {7}, number = {8}, pages = {6884-6893}, doi = {10.1021/nn402062f}, pmid = {23889734}, issn = {1936-086X}, support = {310892//European Research Council/International ; }, mesh = {Amides/*administration &amp; dosage ; Animals ; Dogs ; Drug Carriers ; Drug Delivery Systems ; Humans ; Influenza A virus/*drug effects ; Influenza, Human/*drug therapy ; Madin Darby Canine Kidney Cells ; Microscopy, Fluorescence ; Models, Chemical ; Nanoparticles/*chemistry ; Nanotechnology/*methods ; Particle Size ; Salicylates/*administration &amp; dosage ; Silicon/*chemistry ; Solvents/chemistry ; }, abstract = {Influenza A viruses (IAVs) cause recurrent epidemics in humans, with serious threat of lethal worldwide pandemics. The occurrence of antiviral-resistant virus strains and the emergence of highly pathogenic influenza viruses have triggered an urgent need to develop new anti-IAV treatments. One compound found to inhibit IAV, and other virus infections, is saliphenylhalamide (SaliPhe). SaliPhe targets host vacuolar-ATPase and inhibits acidification of endosomes, a process needed for productive virus infection. The major obstacle for the further development of SaliPhe as antiviral drug has been its poor solubility. Here, we investigated the possibility to increase SaliPhe solubility by loading the compound in thermally hydrocarbonized porous silicon (THCPSi) nanoparticles. SaliPhe-loaded nanoparticles were further investigated for the ability to inhibit influenza A infection in human retinal pigment epithelium and Madin-Darby canine kidney cells, and we show that upon release from THCPSi, SaliPhe inhibited IAV infection in vitro and reduced the amount of progeny virus in IAV-infected cells. Overall, the PSi-based nanosystem exhibited increased dissolution of the investigated anti-IAV drug SaliPhe and displayed excellent in vitro stability, low cytotoxicity, and remarkable reduction of viral load in the absence of organic solvents. This proof-of-principle study indicates that PSi nanoparticles could be used for efficient delivery of antivirals to infected cells.}, }
@article {pmid23887633, year = {2013}, author = {Kakkola, L and Denisova, OV and Tynell, J and Viiliäinen, J and Ysenbaert, T and Matos, RC and Nagaraj, A and Ohman, T and Kuivanen, S and Paavilainen, H and Feng, L and Yadav, B and Julkunen, I and Vapalahti, O and Hukkanen, V and Stenman, J and Aittokallio, T and Verschuren, EW and Ojala, PM and Nyman, T and Saelens, X and Dzeyk, K and Kainov, DE}, title = {Anticancer compound ABT-263 accelerates apoptosis in virus-infected cells and imbalances cytokine production and lowers survival rates of infected mice.}, journal = {Cell death &amp; disease}, volume = {4}, number = {}, pages = {e742}, pmid = {23887633}, issn = {2041-4889}, mesh = {Aniline Compounds/*pharmacology ; Animals ; Antineoplastic Agents/*pharmacology ; Apoptosis/drug effects ; Cell Line, Tumor ; Cytokines/biosynthesis ; Disease Models, Animal ; Influenza A virus/physiology ; Macrophages/metabolism ; Mice ; Neoplasms/*drug therapy/pathology/virology ; Orthomyxoviridae Infections/metabolism/pathology ; Sulfonamides/*pharmacology ; }, abstract = {ABT-263 and its structural analogues ABT-199 and ABT-737 inhibit B-cell lymphoma 2 (Bcl-2), BCL2L1 long isoform (Bcl-xL) and BCL2L2 (Bcl-w) proteins and promote cancer cell death. Here, we show that at non-cytotoxic concentrations, these small molecules accelerate the deaths of non-cancerous cells infected with influenza A virus (IAV) or other viruses. In particular, we demonstrate that ABT-263 altered Bcl-xL interactions with Bcl-2 antagonist of cell death (Bad), Bcl-2-associated X protein (Bax), uveal autoantigen with coiled-coil domains and ankyrin repeats protein (UACA). ABT-263 thereby activated the caspase-9-mediated mitochondria-initiated apoptosis pathway, which, together with the IAV-initiated caspase-8-mediated apoptosis pathway, triggered the deaths of IAV-infected cells. Our results also indicate that Bcl-xL, Bcl-2 and Bcl-w interact with pattern recognition receptors (PRRs) that sense virus constituents to regulate cellular apoptosis. Importantly, premature killing of IAV-infected cells by ABT-263 attenuated the production of key pro-inflammatory and antiviral cytokines. The imbalance in cytokine production was also observed in ABT-263-treated IAV-infected mice, which resulted in an inability of the immune system to clear the virus and eventually lowered the survival rates of infected animals. Thus, the results suggest that the chemical inhibition of Bcl-xL, Bcl-2 and Bcl-w could potentially be hazardous for cancer patients with viral infections.}, }
@article {pmid23872234, year = {2013}, author = {Lowery, RK and Uribe, G and Jimenez, EB and Weiss, MA and Herrera, KJ and Regueiro, M and Herrera, RJ}, title = {Neanderthal and Denisova genetic affinities with contemporary humans: introgression versus common ancestral polymorphisms.}, journal = {Gene}, volume = {530}, number = {1}, pages = {83-94}, doi = {10.1016/j.gene.2013.06.005}, pmid = {23872234}, issn = {1879-0038}, mesh = {Africa South of the Sahara ; Animals ; DNA, Mitochondrial/genetics ; Europe ; Fossils ; Genetic Drift ; Genetics, Population ; Genome, Human ; Humans ; Neanderthals/*genetics ; Pan troglodytes/genetics ; *Phylogeny ; *Polymorphism, Genetic ; }, abstract = {Analyses of the genetic relationships among modern humans, Neanderthals and Denisovans have suggested that 1-4% of the non-Sub-Saharan African gene pool may be Neanderthal derived, while 6-8% of the Melanesian gene pool may be the product of admixture between the Denisovans and the direct ancestors of Melanesians. In the present study, we analyzed single nucleotide polymorphism (SNP) diversity among a worldwide collection of contemporary human populations with respect to the genetic constitution of these two archaic hominins and Pan troglodytes (chimpanzee). We partitioned SNPs into subsets, including those that are derived in both archaic lineages, those that are ancestral in both archaic lineages and those that are only derived in one archaic lineage. By doing this, we have conducted separate examinations of subsets of mutations with higher probabilities of divergent phylogenetic origins. While previous investigations have excluded SNPs from common ancestors in principal component analyses, we included common ancestral SNPs in our analyses to visualize the relative placement of the Neanderthal and Denisova among human populations. To assess the genetic similarities among the various hominin lineages, we performed genetic structure analyses to provide a comparison of genetic patterns found within contemporary human genomes that may have archaic or common ancestral roots. Our results indicate that 3.6% of the Neanderthal genome is shared with roughly 65.4% of the average European gene pool, which clinally diminishes with distance from Europe. Our results suggest that Neanderthal genetic associations with contemporary non-Sub-Saharan African populations, as well as the genetic affinities observed between Denisovans and Melanesians most likely result from the retention of ancient mutations in these populations.}, }
@article {pmid23755774, year = {2013}, author = {Mandras, N and Roana, J and Allizond, V and Pasqualini, D and Crosasso, P and Burlando, M and Banche, G and Denisova, T and Berutti, E and Cuffini, AM}, title = {Antibacterial efficacy and drug-induced tooth discolouration of antibiotic combinations for endodontic regenerative procedures.}, journal = {International journal of immunopathology and pharmacology}, volume = {26}, number = {2}, pages = {557-563}, doi = {10.1177/039463201302600232}, pmid = {23755774}, issn = {0394-6320}, mesh = {Adolescent ; Adult ; Aged ; Anti-Bacterial Agents/*adverse effects ; Ciprofloxacin/adverse effects ; Clarithromycin/adverse effects ; Dental Pulp Cavity/microbiology/*surgery ; Drug Therapy, Combination ; Female ; Fosfomycin/adverse effects ; Humans ; Male ; Metronidazole/adverse effects ; Middle Aged ; Minocycline/adverse effects ; Root Canal Irrigants/*adverse effects ; Root Canal Preparation/*adverse effects ; Therapeutic Irrigation/*adverse effects ; Tooth Discoloration/chemically induced/*prevention &amp; control ; Tooth, Nonvital/microbiology/*surgery ; Young Adult ; }, abstract = {Elimination of microbial contamination from the root canal system is a precondition for successful root canal treatment. Teeth with immature root development, necrotic pulps and apical periodontitis present multiple challenges for successful treatment. Disinfection is achieved by irrigation followed by the placement of an intracanal medicament. A mixture of ciprofloxacin, metronidazole and minocycline (3-MIX S) has been shown to be very effective in eliminating endodontic pathogens in vitro and in vivo. Among the components of the mixture, minocycline can induce tooth discolouration after long-term oral use. Therefore, the elimination of minocycline from the above-mentioned combination has been suggested to prevent the occasion of this undesirable effect. The aim of this study was to investigate the potential antimicrobial efficacy of alternative antibiotic combinations [3-MIX C (clarithromycin); 3-MIX F (fosfomycin)] against bacteria from infected root canals. An additional objective was to evaluate their discolouration potential as possible alternatives to minocycline-based intracanal medicaments. Our in vitro results clearly demonstrated that 3-MIX C and 3-MIX F had a greater antimicrobial activity than 3-MIX S, underlying that clarithromycin still had a higher capacity to kill endodontic pathogens in vitro compared to fosfomycin. Both 3-MIX C and 3-MIX F were able to avoid the permanent staining effect of the crown.}, }
@article {pmid23687020, year = {2013}, author = {Pennisi, E}, title = {Human evolution. More genomes from Denisova Cave show mixing of early human groups.}, journal = {Science (New York, N.Y.)}, volume = {340}, number = {6134}, pages = {799}, doi = {10.1126/science.340.6134.799}, pmid = {23687020}, issn = {1095-9203}, mesh = {Animals ; Anthropology ; *Biological Evolution ; *Caves ; DNA/genetics ; *Genome, Human ; Humans ; Neanderthals/*genetics ; Sequence Analysis, DNA/*methods ; Siberia ; Toe Phalanges ; }, }
@article {pmid23677594, year = {2013}, author = {Dormitzer, PR and Suphaphiphat, P and Gibson, DG and Wentworth, DE and Stockwell, TB and Algire, MA and Alperovich, N and Barro, M and Brown, DM and Craig, S and Dattilo, BM and Denisova, EA and De Souza, I and Eickmann, M and Dugan, VG and Ferrari, A and Gomila, RC and Han, L and Judge, C and Mane, S and Matrosovich, M and Merryman, C and Palladino, G and Palmer, GA and Spencer, T and Strecker, T and Trusheim, H and Uhlendorff, J and Wen, Y and Yee, AC and Zaveri, J and Zhou, B and Becker, S and Donabedian, A and Mason, PW and Glass, JI and Rappuoli, R and Venter, JC}, title = {Synthetic generation of influenza vaccine viruses for rapid response to pandemics.}, journal = {Science translational medicine}, volume = {5}, number = {185}, pages = {185ra68}, doi = {10.1126/scitranslmed.3006368}, pmid = {23677594}, issn = {1946-6242}, support = {HHSN272200900007C//PHS HHS/United States ; HHSO100201000061C//PHS HHS/United States ; }, mesh = {Animals ; Cell Line ; Computer Simulation ; Dogs ; Genes, Synthetic ; Hemagglutinin Glycoproteins, Influenza Virus/genetics ; Humans ; Influenza A Virus, H7N9 Subtype/immunology ; Influenza A virus/*immunology ; Influenza Vaccines/*immunology ; Influenza, Human/*immunology/*prevention &amp; control/virology ; Madin Darby Canine Kidney Cells ; Neuraminidase/genetics ; Pandemics/*prevention &amp; control ; Reassortant Viruses/immunology ; Reproducibility of Results ; Vaccines, Synthetic/*immunology ; Viral Load ; }, abstract = {During the 2009 H1N1 influenza pandemic, vaccines for the virus became available in large quantities only after human infections peaked. To accelerate vaccine availability for future pandemics, we developed a synthetic approach that very rapidly generated vaccine viruses from sequence data. Beginning with hemagglutinin (HA) and neuraminidase (NA) gene sequences, we combined an enzymatic, cell-free gene assembly technique with enzymatic error correction to allow rapid, accurate gene synthesis. We then used these synthetic HA and NA genes to transfect Madin-Darby canine kidney (MDCK) cells that were qualified for vaccine manufacture with viral RNA expression constructs encoding HA and NA and plasmid DNAs encoding viral backbone genes. Viruses for use in vaccines were rescued from these MDCK cells. We performed this rescue with improved vaccine virus backbones, increasing the yield of the essential vaccine antigen, HA. Generation of synthetic vaccine seeds, together with more efficient vaccine release assays, would accelerate responses to influenza pandemics through a system of instantaneous electronic data exchange followed by real-time, geographically dispersed vaccine production.}, }
@article {pmid23666864, year = {2013}, author = {Elhaik, E and Greenspan, E and Staats, S and Krahn, T and Tyler-Smith, C and Xue, Y and Tofanelli, S and Francalacci, P and Cucca, F and Pagani, L and Jin, L and Li, H and Schurr, TG and Greenspan, B and Spencer Wells, R and , }, title = {The GenoChip: a new tool for genetic anthropology.}, journal = {Genome biology and evolution}, volume = {5}, number = {5}, pages = {1021-1031}, pmid = {23666864}, issn = {1759-6653}, support = {T32 MH014592/MH/NIMH NIH HHS/United States ; 098051//Wellcome Trust/United Kingdom ; }, mesh = {*Anthropology, Cultural ; DNA, Mitochondrial/*genetics ; Genes, Y-Linked ; History, Ancient ; Human Genome Project ; Human Migration/history ; Humans ; *Oligonucleotide Array Sequence Analysis ; Polymorphism, Single Nucleotide/*genetics ; }, abstract = {The Genographic Project is an international effort aimed at charting human migratory history. The project is nonprofit and nonmedical, and, through its Legacy Fund, supports locally led efforts to preserve indigenous and traditional cultures. Although the first phase of the project was focused on uniparentally inherited markers on the Y-chromosome and mitochondrial DNA (mtDNA), the current phase focuses on markers from across the entire genome to obtain a more complete understanding of human genetic variation. Although many commercial arrays exist for genome-wide single-nucleotide polymorphism (SNP) genotyping, they were designed for medical genetic studies and contain medically related markers that are inappropriate for global population genetic studies. GenoChip, the Genographic Project's new genotyping array, was designed to resolve these issues and enable higher resolution research into outstanding questions in genetic anthropology. The GenoChip includes ancestry informative markers obtained for over 450 human populations, an ancient human (Saqqaq), and two archaic hominins (Neanderthal and Denisovan) and was designed to identify all known Y-chromosome and mtDNA haplogroups. The chip was carefully vetted to avoid inclusion of medically relevant markers. To demonstrate its capabilities, we compared the FST distributions of GenoChip SNPs to those of two commercial arrays. Although all arrays yielded similarly shaped (inverse J) FST distributions, the GenoChip autosomal and X-chromosomal distributions had the highest mean FST, attesting to its ability to discern subpopulations. The chip performances are illustrated in a principal component analysis for 14 worldwide populations. In summary, the GenoChip is a dedicated genotyping platform for genetic anthropology. With an unprecedented number of approximately 12,000 Y-chromosomal and approximately 3,300 mtDNA SNPs and over 130,000 autosomal and X-chromosomal SNPs without any known health, medical, or phenotypic relevance, the GenoChip is a useful tool for genetic anthropology and population genetics.}, }
@article {pmid23662523, year = {2012}, author = {Zabolotskikh, IB and Musaeva, TS and Denisova, EA}, title = {[Validity of APACHE II, APACHE III, SAPS 2, SAPS 3 and SOFA scales in obstetric patients with sepsis].}, journal = {Anesteziologiia i reanimatologiia}, volume = {}, number = {6}, pages = {55-57}, pmid = {23662523}, issn = {0201-7563}, mesh = {*APACHE ; Adult ; Female ; Hospital Records/statistics &amp; numerical data ; Humans ; *Organ Dysfunction Scores ; Pregnancy ; Pregnancy Complications, Infectious/*diagnosis ; Prognosis ; Reproducibility of Results ; Retrospective Studies ; Sepsis/*diagnosis ; *Severity of Illness Index ; Young Adult ; }, abstract = {RESEARCH OBJECTIVE: to estimate efficiency of APACHE II, APACHE III, SAPS II, SAPS III, SOFA scales for obstetric patients with heavy sepsis.<br><br>MATERIALS AND METHODS: 186 medical cards retrospective analysis of pregnant women with pulmonary sepsis, 40 women with urosepsis and puerperas with abdominal sepsis--66 was performed. Middle age of women was 26.7 (22.4-34.5).<br><br>RESULTS: In population of puerperas with abdominal sepsis APACHE II, APACHE III, SAPS 2, SAPS 3, SOFA scales showed to good calibration, however, high resolution was observed only in APACHE III, SAPS 3 and SOFA (AUROC 0.95; 0.93; 0.92 respectively). APACHE III and SOFA scales provided qualitative prognosis in pregnant women with urosepsis; resolution ratio of these scales considerably exceeds APACHE II, SAPS 2 and SAPS 3 (AUROC 0.73; 0.74; 0.79 respectively). APACHE II scale is inapplicable because of a lack of calibration (X2 = 13.1; p < 0.01), and at other scales (APACHE III, SAPS 2, SAPS 3, SOFA) was observed the insufficient resolution (AUROC < 0.9) in pregnant women with pulmonary sepsis.<br><br>CONCLUSION: Prognostic possibilities assessment of score scales showed that APACHE III, SAPS 3 and SOFA scales can be used for a lethality prognosis for puerperas with abdominal sepsis, in population of pregnant women with urosepsis--only APACHE III and SOFA, and with pulmonary sepsis--SAPS 3 and APACHE III only in case of additional clinical information.}, }
@article {pmid23657884, year = {2013}, author = {Contreras-Galindo, R and Kaplan, MH and He, S and Contreras-Galindo, AC and Gonzalez-Hernandez, MJ and Kappes, F and Dube, D and Chan, SM and Robinson, D and Meng, F and Dai, M and Gitlin, SD and Chinnaiyan, AM and Omenn, GS and Markovitz, DM}, title = {HIV infection reveals widespread expansion of novel centromeric human endogenous retroviruses.}, journal = {Genome research}, volume = {23}, number = {9}, pages = {1505-1513}, pmid = {23657884}, issn = {1549-5469}, support = {R01 AI062248/AI/NIAID NIH HHS/United States ; T32 AI007528/AI/NIAID NIH HHS/United States ; 1F31CA150523-01/CA/NCI NIH HHS/United States ; 5T32AI007528-13/AI/NIAID NIH HHS/United States ; UL1RR24986/RR/NCRR NIH HHS/United States ; U54DA021519/DA/NIDA NIH HHS/United States ; F31 CA150523/CA/NCI NIH HHS/United States ; 3R01CA144043-03S1/CA/NCI NIH HHS/United States ; R01AI062248/AI/NIAID NIH HHS/United States ; P30 ES017885/ES/NIEHS NIH HHS/United States ; U54 DA021519/DA/NIDA NIH HHS/United States ; UL1 RR024986/RR/NCRR NIH HHS/United States ; P30U54ES017885/ES/NIEHS NIH HHS/United States ; R01 CA144043/CA/NCI NIH HHS/United States ; T32 GM007315/GM/NIGMS NIH HHS/United States ; }, mesh = {Animals ; Centromere/genetics/virology ; Chromosomes, Human/genetics/virology ; Endogenous Retroviruses/*genetics ; Evolution, Molecular ; *Genome, Human ; HIV Infections/*genetics ; Hominidae/genetics/virology ; Humans ; Proviruses/*genetics ; *Virus Integration ; tat Gene Products, Human Immunodeficiency Virus/genetics/metabolism ; }, abstract = {Human endogenous retroviruses (HERVs) make up 8% of the human genome. The HERV-K (HML-2) family is the most recent group of these viruses to have inserted into the genome, and we have detected the activation of HERV-K (HML-2) proviruses in the blood of patients with HIV-1 infection. We report that HIV-1 infection activates expression of a novel HERV-K (HML-2) provirus, termed K111, present in multiple copies in the centromeres of chromosomes throughout the human genome yet not annotated in the most recent human genome assembly. Infection with HIV-1 or stimulation with the HIV-1 Tat protein leads to the activation of K111 proviruses. K111 is present as a single copy in the genome of the chimpanzee, yet K111 is not found in the genomes of other primates. Remarkably, K111 proviruses appear in the genomes of the extinct Neanderthal and Denisovan, while modern humans have at least 100 K111 proviruses spread across the centromeres of 15 chromosomes. Our studies suggest that the progenitor K111 integrated before the Homo-Pan divergence and expanded in copy number during the evolution of hominins, perhaps by recombination. The expansion of K111 provides sequence evidence suggesting that recombination between the centromeres of various chromosomes took place during the evolution of humans. K111 proviruses show significant sequence variations in each individual centromere, which may serve as markers in future efforts to annotate human centromere sequences. Further, this work is an example of the potential to discover previously unknown genomic sequences through the analysis of nucleic acids found in the blood of patients.}, }
@article {pmid23553074, year = {2013}, author = {Khairat, R and Ball, M and Chang, CC and Bianucci, R and Nerlich, AG and Trautmann, M and Ismail, S and Shanab, GM and Karim, AM and Gad, YZ and Pusch, CM}, title = {First insights into the metagenome of Egyptian mummies using next-generation sequencing.}, journal = {Journal of applied genetics}, volume = {54}, number = {3}, pages = {309-325}, pmid = {23553074}, issn = {2190-3883}, mesh = {Base Sequence ; Biopsy ; Egypt, Ancient ; Embalming/history/*methods ; Gene Library ; History, Ancient ; Humans ; *Metagenome ; Molecular Sequence Data ; *Mummies ; Phylogeny ; Plasmodium falciparum/genetics ; Polymerase Chain Reaction/methods ; Sequence Analysis, DNA/methods ; Temperature ; Toxoplasma/genetics ; }, abstract = {We applied, for the first time, next-generation sequencing (NGS) technology on Egyptian mummies. Seven NGS datasets obtained from five randomly selected Third Intermediate to Graeco-Roman Egyptian mummies (806 BC-124AD) and two unearthed pre-contact Bolivian lowland skeletons were generated and characterised. The datasets were contrasted to three recently published NGS datasets obtained from cold-climate regions, i.e. the Saqqaq, the Denisova hominid and the Alpine Iceman. Analysis was done using one million reads of each newly generated or published dataset. Blastn and megablast results were analysed using MEGAN software. Distinct NGS results were replicated by specific and sensitive polymerase chain reaction (PCR) protocols in ancient DNA dedicated laboratories. Here, we provide unambiguous identification of authentic DNA in Egyptian mummies. The NGS datasets showed variable contents of endogenous DNA harboured in tissues. Three of five mummies displayed a human DNA proportion comparable to the human read count of the Saqqaq permafrost-preserved specimen. Furthermore, a metagenomic signature unique to mummies was displayed. By applying a "bacterial fingerprint", discrimination among mummies and other remains from warm areas outside Egypt was possible. Due to the absence of an adequate environment monitoring, a bacterial bloom was identified when analysing different biopsies from the same mummies taken after a lapse of time of 1.5 years. Plant kingdom representation in all mummy datasets was unique and could be partially associated with their use in embalming materials. Finally, NGS data showed the presence of Plasmodium falciparum and Toxoplasma gondii DNA sequences, indicating malaria and toxoplasmosis in these mummies. We demonstrate that endogenous ancient DNA can be extracted from mummies and serve as a proper template for the NGS technique, thus, opening new pathways of investigation for future genome sequencing of ancient Egyptian individuals.}, }
@article {pmid23415986, year = {2013}, author = {Malyarchuk, B and Derenko, M and Denisova, G}, title = {Phylogeny and genetic history of the Siberian salamander (Salamandrella keyserlingii, Dybowski, 1870) inferred from complete mitochondrial genomes.}, journal = {Molecular phylogenetics and evolution}, volume = {67}, number = {2}, pages = {348-357}, doi = {10.1016/j.ympev.2013.02.004}, pmid = {23415986}, issn = {1095-9513}, mesh = {Animals ; Genetics, Population ; *Genome, Mitochondrial ; Haplotypes ; *Phylogeny ; Russia ; Sequence Analysis, DNA ; Siberia ; *Urodela/classification/genetics ; }, abstract = {We assessed phylogeny of the Siberian salamander (Salamandrella keyserlingii, Dybowski, 1870), the most northern ectothermic, terrestrial vertebrate in Eurasia, by sequence analysis of complete mitochondrial genomes in 26 specimens from different localities (China, Khabarovsk region, Sakhalin, Yakutia, Magadan region, Chukotka, Kamchatka, Ural, European part of Russia). In addition, a complete mitochondrial genome of the Schrenck salamander, Salamandrella schrenckii, was determined for the first time. Bayesian phylogenetic analysis of the entire mtDNA genomes of S. keyserlingii demonstrates that two haplotype clades, AB and C, radiated about 1.4 million years ago (Mya). Bayesian skyline plots of population size change through time show an expansion around 250 thousand years ago (kya) and then a decline around the Last Glacial Maximum (25 kya) with subsequent restoration of population size. Climatic changes during the Quaternary period have dramatically affected the population genetic structure of the Siberian salamanders. In addition, complete mtDNA sequence analysis allowed us to recognize that the vast area of Northern Eurasia was colonized only by the Siberian salamander clade C1b during the last 150 kya. Meanwhile, we were unable to find evidence of molecular adaptation in this clade by analyzing the whole mitochondrial genomes of the Siberian salamanders.}, }
@article {pmid23413113, year = {2012}, author = {Paixão-Côrtes, VR and Viscardi, LH and Salzano, FM and Hünemeier, T and Bortolini, MC}, title = {Homo sapiens, Homo neanderthalensis and the Denisova specimen: New insights on their evolutionary histories using whole-genome comparisons.}, journal = {Genetics and molecular biology}, volume = {35}, number = {4 (suppl)}, pages = {904-911}, pmid = {23413113}, issn = {1415-4757}, abstract = {After a brief review of the most recent findings in the study of human evolution, an extensive comparison of the complete genomes of our nearest relative, the chimpanzee (Pan troglodytes), of extant Homo sapiens, archaic Homo neanderthalensis and the Denisova specimen were made. The focus was on non-synonymous mutations, which consequently had an impact on protein levels and these changes were classified according to degree of effect. A total of 10,447 non-synonymous substitutions were found in which the derived allele is fixed or nearly fixed in humans as compared to chimpanzee. Their most frequent location was on chromosome 21. Their presence was then searched in the two archaic genomes. Mutations in 381 genes would imply radical amino acid changes, with a fraction of these related to olfaction and other important physiological processes. Eight new alleles were identified in the Neanderthal and/or Denisova genetic pools. Four others, possibly affecting cognition, occured both in the sapiens and two other archaic genomes. The selective sweep that gave rise to Homo sapiens could, therefore, have initiated before the modern/archaic human divergence.}, }
@article {pmid23402178, year = {2012}, author = {Malanichevskaia, TG and Narykov, RKh and Denisova, SN}, title = {[Peculiarities of diet therapy in children with duodenal ulcers associated with the genus Candida].}, journal = {Eksperimental'naia i klinicheskaia gastroenterologiia = Experimental &amp; clinical gastroenterology}, volume = {}, number = {5}, pages = {87-90}, pmid = {23402178}, issn = {1682-8658}, mesh = {Adolescent ; Animals ; Biopsy ; *Candida ; Candidiasis/*diet therapy/microbiology/pathology ; Child ; Duodenal Ulcer/*diet therapy/microbiology/pathology ; Female ; Goats ; Humans ; Intestinal Mucosa/microbiology/pathology ; Male ; Milk ; }, abstract = {In children with relapsing DU in 50.8% cases there is a complicated disease course with Candida infection, as evidenced by the positive results of mycological examination of biopsy specimens of duodenal mucosa and detection of circulating Candida antigen in serum. The use in the complex therapy in children with DU associated with Candida species of instant New Zealand goat milk "Amalteya" leads to an overall therapeutic effect in 81.3% cases, which is manifested in the reduction of the duration of relapse 1.4 times prolongation of remission in 3 times and milder disease. Carried out in the dynamics fibrogastroduodenoscopy after treatment showed that 100% of the observed total endoscopic remission which is manifested in the disappearance of opalescent gray overlay on the bottom of the ulcer and ulcer epithelization.}, }
@article {pmid23315957, year = {2013}, author = {Mendez, FL and Watkins, JC and Hammer, MF}, title = {Neandertal origin of genetic variation at the cluster of OAS immunity genes.}, journal = {Molecular biology and evolution}, volume = {30}, number = {4}, pages = {798-801}, doi = {10.1093/molbev/mst004}, pmid = {23315957}, issn = {1537-1719}, mesh = {2',5'-Oligoadenylate Synthetase/*genetics ; Animals ; Evolution, Molecular ; Genetic Loci/*immunology ; Genetic Speciation ; Gorilla gorilla/genetics ; Haplotypes ; Humans ; Linkage Disequilibrium ; Models, Genetic ; Multigene Family ; Neanderthals/*genetics ; Pan troglodytes/genetics ; Phylogeography ; *Polymorphism, Single Nucleotide ; }, abstract = {Analyses of ancient DNA from extinct humans reveal signals of at least two independent hybridization events in the history of non-African populations. To date, there are very few examples of specific genetic variants that have been rigorously identified as introgressive. Here, we survey DNA sequence variation in the OAS gene cluster on chromosome 12 and provide strong evidence that a haplotype extending for ~185 kb introgressed from Neandertals. This haplotype is nearly restricted to Eurasians and is estimated to have diverged from the Neandertal sequence ~125 kya. Despite the potential for novel functional variation, the observed frequency of this haplotype is consistent with neutral introgression. This is the second locus in the human genome, after STAT2, carrying distinct haplotypes that appear to have introgressed separately from both Neandertals and Denisova.}, }
@article {pmid24933983, year = {2013}, author = {Reshetnikov, OV and Kurilovich, SA and Denisova, DV}, title = {[Symptoms of gastroesophageal reflux disease and associated factors in adolescents: population survey].}, journal = {Eksperimental'naia i klinicheskaia gastroenterologiia = Experimental &amp; clinical gastroenterology}, volume = {}, number = {12}, pages = {8-14}, pmid = {24933983}, issn = {1682-8658}, mesh = {Adolescent ; Antibodies, Bacterial/blood ; Female ; *Gastroesophageal Reflux/diagnosis/epidemiology/etiology ; Helicobacter Infections/complications/epidemiology/microbiology ; Helicobacter pylori/isolation &amp; purification ; Humans ; Male ; *Population Surveillance ; Prevalence ; Risk Factors ; Sex Factors ; Siberia/epidemiology ; Surveys and Questionnaires ; }, abstract = {OBJECTIVES: Gastroesophageal reflux (GER) is frequent among the general population affecting 10-20% of adults. However, there is a notable lack of epidemiological data describing prevalence of GER in children. The aims of the present study were to assess the prevalence of GER symptoms (GERS) in adolescents and to evaluate factors associated with GERS including markers of H. pylori infection.<br><br>METHODS: All school students in grades 9-11 in four randomly selected secondary schools in Novosibirsk participated (449 adolescents, 189 boys, 260 girls aged 14-17). They completed the Bowel Disease Questionnaire, life-style questionnaire, and sera were tested for antibodies against Helicobacter pylori infection.<br><br>RESULTS: Overall, 60% of adolescents experienced GERS (heartburn and/or acid regurgitation) over the previous year. GER symptoms on a monthly basis were reported by 22% of students, weekly GERS were reported by 9% of adolescents with the same frequency in both genders. GERS were related to family history of dyspepsia or GER, mother's lower educational attainment, overweight, unhealthy eating patterns, alcohol consumption, smoking, and H. pylori infection, as well as concomitant dyspepsia and irritable bowel syndrome. The majority of associations were more prominent in girls. Visiting a physician, endoscopic study, and school absenteeism were reported in the last year more frequently by adolescents with GERS vs those without GERS.<br><br>CONCLUSIONS: Gastroesophageal reflux symptoms are frequent among the adolescent population and result in frequent use of health care resources. Some precipitated factors found in this study are modifiable and may be corrected in adolescent population.}, }
@article {pmid24933954, year = {2013}, author = {Malanicheva, TG and Ziatdinova, NV and Denisova, SN}, title = {[Prevalence and risk factors of gastroduodenal and biliary system diseases in infants and preschool children].}, journal = {Eksperimental'naia i klinicheskaia gastroenterologiia = Experimental &amp; clinical gastroenterology}, volume = {}, number = {8}, pages = {77-80}, pmid = {24933954}, issn = {1682-8658}, mesh = {Age Factors ; Bile Duct Diseases/*epidemiology ; Child ; Child, Preschool ; Duodenal Diseases/*epidemiology ; Female ; Humans ; Infant ; Male ; Prevalence ; Risk Factors ; Stomach Diseases/*epidemiology ; }, abstract = {To study the prevalence and risk factors of digestive diseases in children performed continuous screening questionnaire 786 children from 1.5 to 7 years. Were Revealed that 47.1 +/- 3.5% of children have symptoms of diseases of the gastrointestinal tract and biliary system. This symptoms occur in children aged 4 to 7 years in 2.3 times more frequently than in children from 1.5 to 3 years. In-depth study was revealed that the frequency of biliary dyskinesia (BD) was 33 +/- 3.3%, chronic gastritis (CG) and gastroduodenitis (CGD)--6.2 +/- 1.8%, chronic cholecystitis--4.2 +/- 1.4%, functional disorders of the stomach (FSD)--2.8 +/- 1.2%, and peptic duodenal ulcer (DU)--0.3 + 0.4%. In the structure of digestive diseases in children from 1.5 to 7 years on the 1st place are BD--70% on the 2nd--CG and CGD--14.7% on the 3rd chronic cholecystitis--8.9%, on the 4th FSD-5.9% and 5th place DU-0.5%. For dispensary observation in the children's clinic with digestive diseases consist of only 7.9 +/- 1.9% of children, which is 5.9 times lower than according to the active diagnostics data. The leading medical and social risk factors and their complex influence on the formation of digestive disorders in infants and preschool children.}, }
@article {pmid24772875, year = {2013}, author = {Denisova, TL and Tiul'tiaeva, LA and Lipatova, TE and Bakulev, AL and Alipova, LN and Apanasevich, AV and Bezrodnaia, LA and Borisova, EA}, title = {[Tertiary syphilis of the pancreas and liver in 82-year-old patient: case study].}, journal = {Eksperimental'naia i klinicheskaia gastroenterologiia = Experimental &amp; clinical gastroenterology}, volume = {}, number = {7}, pages = {41-43}, pmid = {24772875}, issn = {1682-8658}, mesh = {Aged, 80 and over ; Anti-Bacterial Agents/administration &amp; dosage/therapeutic use ; Ceftriaxone/administration &amp; dosage/therapeutic use ; Diagnosis, Differential ; *Diagnostic Errors ; Humans ; Liver Diseases/*diagnosis/drug therapy/pathology ; Liver Neoplasms/*diagnosis/secondary/surgery ; Male ; Pancreatic Diseases/*diagnosis/drug therapy/pathology ; Pancreatic Neoplasms/*diagnosis/pathology/surgery ; Syphilis/*diagnosis/drug therapy/pathology ; Syphilis Serodiagnosis ; Treatment Outcome ; }, abstract = {It has been described a clinical case of late diagnosis of syphilis of the pancreas and liver of elderly patients. Two years before that it was wrongly diagnosed with cancer of the pancreas with liver metastases, and the patient was operated on with the imposition of cholecystostomy. It was conducted appropriate therapy and reconstructive surgery after verification of the diagnosis of syphilis of the pancreas and liver.}, }
@article {pmid24640735, year = {2013}, author = {Alekseeva, EI and Baranov, AA and Mitenko, EV and Bzarova, TM and Valieva, SI and Denisova, RV and Isaeva, KB and Chistiakova, EG and Sleptsova, TV and Taĭbulatov, NI and Zelikovich, EI and Kurilenkov, GV}, title = {[Efficiency and safety of human monoclonal antibodies to TNF-alpha in children with juvenile idiopathic arthritis in primary and secondary ineffectiveness of other biologicals].}, journal = {Vestnik Rossiiskoi akademii meditsinskikh nauk}, volume = {}, number = {11}, pages = {73-82}, pmid = {24640735}, issn = {0869-6047}, mesh = {Adalimumab ; Adolescent ; Antibodies, Monoclonal, Humanized/*immunology/therapeutic use ; Antirheumatic Agents/therapeutic use ; Arthritis, Juvenile/*drug therapy ; Biological Factors/*therapeutic use ; Child ; Child, Preschool ; Female ; Follow-Up Studies ; Humans ; Male ; Retrospective Studies ; Treatment Outcome ; Tumor Necrosis Factor-alpha/*antagonists &amp; inhibitors/immunology ; }, abstract = {PURPOSE: To evaluate the safety and efficiency of adalimumab in children with severe refractory JIA with primary inefficiency, partial effect or loss of the effectiveness of other biologicals.<br><br>PATIENTS AND METHODS: The article presents the results of the retrospective observational study of the efficacy and safety of adalimumab in 68 patients aged 10 (3, 17) years with various embodiments of JIA, with the primary inefficiency or partial or loss of the effectiveness of other biologicals. JIA diagnosis established on the basis of criteria ILAR (International League of Associations for Rheumatology).<br><br>RESULTS: Efficacy was assessed during 1 year in 68 and 2 years--in 56 patients . At the 24th week we observed the improvement by criteria AKR 30, 50.70 in 100, 91 and 74% of patients, respectively, and at the 52th week--in 100, 96 and 90%, respectively. Inactive disease status was recorded in 55.8, 66.1 and 98.2% of study participants after 6 months, 1 and 2 years, respectively. Remission was achieved in 55.8 and 96.4% of patients after 1 and 2 years of observation, respectively.<br><br>CONCLUSIONS: Adalimumab was effective and well tolerated by patients with primary inefficiency, partial and loss of efficiency of other biologicals. In clinical practice, patients with non-systemic JIA transition to the second TNF-alpha blocker can restore the biological effect of the first drug without increasing the frequency of infectious AEs.}, }
@article {pmid23210182, year = {2012}, author = {Torubarov, FS and Isaeva, NA and Zvereva, ZF and Denisova, EA and Metliaeva, NA}, title = {[Contemporary legislation and importance of psychophysiologic examination in system of medical support for workers engaged into production with radiation and nuclear danger].}, journal = {Meditsina truda i promyshlennaia ekologiia}, volume = {}, number = {10}, pages = {35-39}, pmid = {23210182}, issn = {1026-9428}, mesh = {*General Adaptation Syndrome/diagnosis/etiology/prevention &amp; control ; Humans ; Mental Health/*legislation &amp; jurisprudence ; Nuclear Energy/legislation &amp; jurisprudence ; Nuclear Reactors/legislation &amp; jurisprudence ; *Occupational Diseases/diagnosis/etiology/prevention &amp; control ; *Occupational Exposure/adverse effects/legislation &amp; jurisprudence/prevention &amp; control ; Preventive Health Services/legislation &amp; jurisprudence/methods ; Radiation Protection/legislation &amp; jurisprudence/methods/standards ; *Radioactive Hazard Release/legislation &amp; jurisprudence/prevention &amp; control/psychology ; Russia ; Stress, Physiological/*radiation effects ; Work Capacity Evaluation ; }, abstract = {In accordance with contemporary legislation, the article covers materials on specification and approbation of concept model for psychophysiologic examination in medical establishments during medical examination of workers engaged into production with raidation and nuclear danger. The authors defined methodology, examination methods and designed an order of psychophysiologic examination. The psychophysiologic examination and purpose-oriented rehabilitation appeared efficient.}, }
@article {pmid23112842, year = {2012}, author = {McIntosh, AM and Bennett, C and Dickson, D and Anestis, SF and Watts, DP and Webster, TH and Fontenot, MB and Bradley, BJ}, title = {The apolipoprotein E (APOE) gene appears functionally monomorphic in chimpanzees (Pan troglodytes).}, journal = {PloS one}, volume = {7}, number = {10}, pages = {e47760}, pmid = {23112842}, issn = {1932-6203}, support = {RR016483/RR/NCRR NIH HHS/United States ; RR014491/RR/NCRR NIH HHS/United States ; U42 RR015087/RR/NCRR NIH HHS/United States ; RR015087/RR/NCRR NIH HHS/United States ; C06 RR014491/RR/NCRR NIH HHS/United States ; //Howard Hughes Medical Institute/United States ; C06 RR016483/RR/NCRR NIH HHS/United States ; }, mesh = {Animals ; Apolipoproteins E/*genetics ; Genetic Variation ; Hominidae/genetics ; Humans ; Mutation ; Pan troglodytes/*genetics ; Polymorphism, Single Nucleotide ; Sequence Analysis ; }, abstract = {BACKGROUND: The human apolipoprotein E (APOE) gene is polymorphic, with three primary alleles (E2, E3, E4) that differ at two key non-synonymous sites. These alleles are functionally different in how they bind to lipoproteins, and this genetic variation is associated with phenotypic variation for several medical traits, including cholesterol levels, cardiovascular health, Alzheimer's disease risk, and longevity. The relative frequencies of these alleles vary across human populations, and the evolution and maintenance of this diversity is much debated. Previous studies comparing human and chimpanzee APOE sequences found that the chimpanzee sequence is most similar to the human E4 allele, although the resulting chimpanzee protein might function like the protein coded for by the human E3 allele. However, these studies have used sequence data from a single chimpanzee and do not consider whether chimpanzees, like humans, show intra-specific and subspecific variation at this locus.<br><br>To examine potential intraspecific variation, we sequenced the APOE gene of 32 chimpanzees. This sample included 20 captive individuals representing the western subspecies (P. troglodytes verus) and 12 wild individuals representing the eastern subspecies (P. t. schweinfurthii). Variation in our resulting sequences was limited to one non-coding, intronic SNP, which showed fixed differences between the two subspecies. We also compared APOE sequences for all available ape genera and fossil hominins. The bonobo APOE protein is identical to that of the chimpanzee, and the Denisovan APOE exhibits all four human-specific, non-synonymous changes and appears functionally similar to the human E4 allele.<br><br>CONCLUSIONS: We found no coding variation within and between chimpanzee populations, suggesting that the maintenance of functionally diverse APOE polymorphisms is a unique feature of human evolution.}, }
@article {pmid22946817, year = {2012}, author = {Bokma, F and van den Brink, V and Stadler, T}, title = {Unexpectedly many extinct hominins.}, journal = {Evolution; international journal of organic evolution}, volume = {66}, number = {9}, pages = {2969-2974}, doi = {10.1111/j.1558-5646.2012.01660.x}, pmid = {22946817}, issn = {1558-5646}, mesh = {Animals ; *Extinction, Biological ; *Genetic Speciation ; Hominidae/*genetics ; Humans ; Models, Genetic ; }, abstract = {Recent studies indicate that Neanderthal and Denisova hominins may have been separate species, while debate continues on the status of Homo floresiensis. The decade-long debate between "splitters," who recognize over 20 hominin species, and "lumpers," who maintain that all these fossils belong to just a few lineages, illustrates that we do not know how many extinct hominin species to expect. Here, we present probability distributions for the number of speciation events and the number of contemporary species along a branch of a phylogeny. With estimates of hominin speciation and extincton rates, we then show that the expected total number of extinct hominin species is 8, but may be as high as 27. We also show that it is highly unlikely that three very recent species disappeared due to natural, background extinction. This may indicate that human-like remains are too easily considered distinct species. Otherwise, the evidence suggesting that Neanderthal and the Denisova hominin represent distinct species implies a recent wave of extinctions, ostensibly driven by the only survivor, H. sapiens.}, }
@article {pmid22936568, year = {2012}, author = {Meyer, M and Kircher, M and Gansauge, MT and Li, H and Racimo, F and Mallick, S and Schraiber, JG and Jay, F and Prüfer, K and de Filippo, C and Sudmant, PH and Alkan, C and Fu, Q and Do, R and Rohland, N and Tandon, A and Siebauer, M and Green, RE and Bryc, K and Briggs, AW and Stenzel, U and Dabney, J and Shendure, J and Kitzman, J and Hammer, MF and Shunkov, MV and Derevianko, AP and Patterson, N and Andrés, AM and Eichler, EE and Slatkin, M and Reich, D and Kelso, J and Pääbo, S}, title = {A high-coverage genome sequence from an archaic Denisovan individual.}, journal = {Science (New York, N.Y.)}, volume = {338}, number = {6104}, pages = {222-226}, pmid = {22936568}, issn = {1095-9203}, support = {R01-GM40282/GM/NIGMS NIH HHS/United States ; R01 GM100233/GM/NIGMS NIH HHS/United States ; R01 GM040282/GM/NIGMS NIH HHS/United States ; GM100233/GM/NIGMS NIH HHS/United States ; //Howard Hughes Medical Institute/United States ; }, mesh = {Alleles ; Animals ; Base Sequence ; Fossils ; Gene Flow ; Gene Library ; *Genetic Variation ; Genome, Human/*genetics ; *Heterozygote ; Humans ; Molecular Sequence Data ; Neanderthals/*genetics ; Sequence Analysis, DNA ; }, abstract = {We present a DNA library preparation method that has allowed us to reconstruct a high-coverage (30×) genome sequence of a Denisovan, an extinct relative of Neandertals. The quality of this genome allows a direct estimation of Denisovan heterozygosity indicating that genetic diversity in these archaic hominins was extremely low. It also allows tentative dating of the specimen on the basis of "missing evolution" in its genome, detailed measurements of Denisovan and Neandertal admixture into present-day human populations, and the generation of a near-complete catalog of genetic changes that swept to high frequency in modern humans since their divergence from Denisovans.}, }
@article {pmid22910914, year = {2012}, author = {Denisova, OV and Kakkola, L and Feng, L and Stenman, J and Nagaraj, A and Lampe, J and Yadav, B and Aittokallio, T and Kaukinen, P and Ahola, T and Kuivanen, S and Vapalahti, O and Kantele, A and Tynell, J and Julkunen, I and Kallio-Kokko, H and Paavilainen, H and Hukkanen, V and Elliott, RM and De Brabander, JK and Saelens, X and Kainov, DE}, title = {Obatoclax, saliphenylhalamide, and gemcitabine inhibit influenza a virus infection.}, journal = {The Journal of biological chemistry}, volume = {287}, number = {42}, pages = {35324-35332}, pmid = {22910914}, issn = {1083-351X}, support = {P01 CA095471/CA/NCI NIH HHS/United States ; R01 CA090349/CA/NCI NIH HHS/United States ; CA90349/CA/NCI NIH HHS/United States ; CA95471/CA/NCI NIH HHS/United States ; }, mesh = {Amides/*pharmacology ; Animals ; Antiviral Agents/*pharmacology ; Chlorocebus aethiops ; Deoxycytidine/*analogs &amp; derivatives/pharmacology ; Dogs ; Humans ; Influenza A Virus, H3N2 Subtype/*drug effects/*physiology ; Influenza, Human/*drug therapy/metabolism ; Myeloid Cell Leukemia Sequence 1 Protein ; Proto-Oncogene Proteins c-bcl-2/antagonists &amp; inhibitors/metabolism ; Pyrroles/*pharmacology ; RNA, Viral/biosynthesis ; Salicylates/*pharmacology ; Vero Cells ; Virus Replication ; }, abstract = {Influenza A viruses (IAVs) infect humans and cause significant morbidity and mortality. Different treatment options have been developed; however, these were insufficient during recent IAV outbreaks. Here, we conducted a targeted chemical screen in human nonmalignant cells to validate known and search for novel host-directed antivirals. The screen validated saliphenylhalamide (SaliPhe) and identified two novel anti-IAV agents, obatoclax and gemcitabine. Further experiments demonstrated that Mcl-1 (target of obatoclax) provides a novel host target for IAV treatment. Moreover, we showed that obatoclax and SaliPhe inhibited IAV uptake and gemcitabine suppressed viral RNA transcription and replication. These compounds possess broad spectrum antiviral activity, although their antiviral efficacies were virus-, cell type-, and species-specific. Altogether, our results suggest that phase II obatoclax, investigational SaliPhe, and FDA/EMEA-approved gemcitabine represent potent antiviral agents.}, }
@article {pmid22883142, year = {2012}, author = {Mendez, FL and Watkins, JC and Hammer, MF}, title = {A haplotype at STAT2 Introgressed from neanderthals and serves as a candidate of positive selection in Papua New Guinea.}, journal = {American journal of human genetics}, volume = {91}, number = {2}, pages = {265-274}, pmid = {22883142}, issn = {1537-6605}, mesh = {Adaptation, Biological/genetics ; Animals ; Base Sequence ; Evolution, Molecular ; Gene Components ; *Genetics, Population ; Genotype ; Haplotypes/*genetics ; Humans ; Linkage Disequilibrium ; Molecular Sequence Data ; Mutation/genetics ; Neanderthals/*genetics ; Papua New Guinea ; *Phylogeny ; Receptor, ErbB-3/genetics ; STAT2 Transcription Factor/*genetics ; *Selection, Genetic ; Sequence Analysis, DNA ; }, abstract = {Signals of archaic admixture have been identified through comparisons of the draft Neanderthal and Denisova genomes with those of living humans. Studies of individual loci contributing to these genome-wide average signals are required for characterization of the introgression process and investigation of whether archaic variants conferred an adaptive advantage to the ancestors of contemporary human populations. However, no definitive case of adaptive introgression has yet been described. Here we provide a DNA sequence analysis of the innate immune gene STAT2 and show that a haplotype carried by many Eurasians (but not sub-Saharan Africans) has a sequence that closely matches that of the Neanderthal STAT2. This haplotype, referred to as N, was discovered through a resequencing survey of the entire coding region of STAT2 in a global sample of 90 individuals. Analyses of publicly available complete genome sequence data show that haplotype N shares a recent common ancestor with the Neanderthal sequence (~80 thousand years ago) and is found throughout Eurasia at an average frequency of ~5%. Interestingly, N is found in Melanesian populations at ~10-fold higher frequency (~54%) than in Eurasian populations. A neutrality test that controls for demography rejects the hypothesis that a variant of N rose to high frequency in Melanesia by genetic drift alone. Although we are not able to pinpoint the precise target of positive selection, we identify nonsynonymous mutations in ERBB3, ESYT1, and STAT2-all of which are part of the same 250 kb introgressive haplotype-as good candidates.}, }
@article {pmid22810113, year = {2012}, author = {Malyarchuk, BA and Derenko, M and Denisova, G}, title = {On the Y-chromosome haplogroup C3c classification.}, journal = {Journal of human genetics}, volume = {57}, number = {10}, pages = {685-686}, doi = {10.1038/jhg.2012.93}, pmid = {22810113}, issn = {1435-232X}, mesh = {Chromosomes, Human, Y/*genetics ; Ethnic Groups/*genetics ; Europe, Eastern ; Far East ; Genetic Markers ; Genetics, Population/*methods ; *Haplotypes ; Humans ; Male ; Minisatellite Repeats ; Mutation ; Phylogeny ; Polymorphism, Single Nucleotide ; Siberia ; }, abstract = {As there are ambiguities in classification of the Y-chromosome haplogroup C3c, relatively frequent in populations of Northern Asia, we analyzed all three haplogroup-defining markers M48, M77 and M86 in C3-M217-individuals from Siberia, Eastern Asia and Eastern Europe. We have found that haplogroup C3c is characterized by the derived state at M48, whereas mutations at both M77 and M86 define subhaplogroup C3c1. The branch defined by M48 alone would belong to subhaplogroup C3c*, characteristic for some populations of Central and Eastern Siberia, such as Koryaks, Evens, Evenks and Yukaghirs. Subhaplogroup C3c* individuals could be considered as remnants of the Neolithic population of Siberia, based on the age of C3c*-short tandem repeat variation amounting to 4.5 ± 2.4 thousand years.}, }
@article {pmid22808780, year = {2012}, author = {Malanicheva, TG and Ziatdinova, NV and Denisova, SN}, title = {[Prevalence and risk factors of gastroduodenal and biliary system diseases in infants and preschool children].}, journal = {Eksperimental'naia i klinicheskaia gastroenterologiia = Experimental &amp; clinical gastroenterology}, volume = {}, number = {1}, pages = {55-58}, pmid = {22808780}, issn = {1682-8658}, mesh = {Age Factors ; *Biliary Tract Diseases/diagnosis/epidemiology/etiology ; Child ; Child, Preschool ; *Duodenal Diseases/diagnosis/epidemiology/etiology ; Humans ; Infant ; Mass Screening ; Prevalence ; Risk Factors ; Russia/epidemiology ; *Stomach Diseases/diagnosis/epidemiology/etiology ; }, abstract = {Gastroduodenal pathology, functional disorders and inflammatory diseases of the biliary tract take leading position among the diseases of the digestive system in children. Precisely these clinical forms are more intensive then other nosology. Continuous screening questionnaire of 786 children aged 1.5 to 7 years was carried out to study the prevalence and risk factors for diseases of the digestive system in children. Based on retrieved data was determined that 47,1 +/- 3,5% of children had symptoms of gastro-intestinal tract and biliary system diseases. They met 2.3 times more often in children aged 4 to 7 years, than in children from 1.5 to 3 years old. Immerced examination revealed that the incidence of dyskinesia of the biliary tract was 33 +/- 3,3%, chronic gastritis (CG) and gastroduodenitis (GDD)--6,2 +/- 1,8%, chronic cholecystitis--4,2 +/- 1,4%, functional disorders of the stomach (FGD)--2,8 +/- 1,2% and duodenal ulcer (DU)--0,3 +/- 0,4% of cases. In the structure of digestive diseases in children from 1.5 to 7 years in the first place were DBT--70%, the second--CGD and CG--14.7%--the third chronic cholecystitis--8.9%, the fourth--FGD--5.9% and in fifth place--DU-0.5% of cases. At the care record in the pediatric clinic with diseases of the digestive system were registered only 7,9 +/- 1,9% of children, which is 5.9 times lower accordinig to data of active diagnostic. The leading medical and social risk factors and their complex influence on the formation of digestive diseases in infants and preschool children.}, }
@article {pmid22781494, year = {2012}, author = {Belousov, AB and Wang, Y and Song, JH and Denisova, JV and Berman, NE and Fontes, JD}, title = {Neuronal gap junctions play a role in the secondary neuronal death following controlled cortical impact.}, journal = {Neuroscience letters}, volume = {524}, number = {1}, pages = {16-19}, pmid = {22781494}, issn = {1872-7972}, support = {P30 HD002528/HD/NICHD NIH HHS/United States ; R01 NS064256/NS/NINDS NIH HHS/United States ; R21 NS076925/NS/NINDS NIH HHS/United States ; HD002528/HD/NICHD NIH HHS/United States ; }, mesh = {Animals ; Brain/metabolism/*pathology ; Brain Injuries/metabolism/*pathology ; Cell Death ; Cerebral Cortex/metabolism/pathology ; Connexins/*genetics ; Gap Junctions/drug effects/*physiology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Neurons/pathology/*physiology ; Receptors, Metabotropic Glutamate/antagonists &amp; inhibitors ; }, abstract = {In the mammalian CNS, excessive release of glutamate and overactivation of glutamate receptors are responsible for the secondary (delayed) neuronal death following neuronal injury, including ischemia, traumatic brain injury (TBI) and epilepsy. Recent studies in mice showed a critical role for neuronal gap junctions in NMDA receptor-mediated excitotoxicity and ischemia-mediated neuronal death. Here, using controlled cortical impact (CCI) in adult mice, as a model of TBI, and Fluoro-Jade B staining for analysis of neuronal death, we set to determine whether neuronal gap junctions play a role in the CCI-mediated secondary neuronal death. We report that 24h post-CCI, substantial neuronal death is detected in a number of brain regions outside the injury core, including the striatum. The striatal neuronal death is reduced both in wild-type mice by systemic administration of mefloquine (a relatively selective blocker of neuronal gap junctions) and in knockout mice lacking connexin 36 (neuronal gap junction protein). It is also reduced by inactivation of group II metabotropic glutamate receptors (with LY341495) which, as reported previously, control the rapid increase in neuronal gap junction coupling following different types of neuronal injury. The results suggest that neuronal gap junctions play a critical role in the CCI-induced secondary neuronal death.}, }
@article {pmid22702107, year = {2012}, author = {Bugaeva, LI and Denisova, TD and Spasov, AA}, title = {[Effect of ladasten on antenatal and postnatal development].}, journal = {Eksperimental'naia i klinicheskaia farmakologiia}, volume = {75}, number = {4}, pages = {23-25}, pmid = {22702107}, issn = {0869-2092}, mesh = {Adamantane/administration &amp; dosage/*analogs &amp; derivatives ; Animals ; Female ; Fetal Development/*drug effects ; Pregnancy ; Rats ; Weight Gain/*drug effects ; }, abstract = {Positive effects of ladasten on both antenatal and postnatal development have been established in experiments on pregnant female rats. Under the action of this drug, the number of resorption events decreases and process of antenatal development of fetuses is activated. In the postnatal period, increased weight gain and accelerated physical development has been observed in the progeny of rats treated with ladasten.}, }
@article {pmid22686063, year = {2012}, author = {Kul'bitskiĭ, BN and Larev, ZV and Fedulova, MV and Denisova, OP and Bogomolov, DV}, title = {[Pathology of the heart conducting system in the thanatogenesis of sudden death from alcoholic cardiomyopathy and coronary heart disease].}, journal = {Sudebno-meditsinskaia ekspertiza}, volume = {55}, number = {2}, pages = {62-65}, pmid = {22686063}, issn = {0039-4521}, mesh = {Cardiomyopathy, Alcoholic/complications/*pathology ; Coronary Disease/complications/*pathology ; Death, Sudden/etiology/*pathology ; *Forensic Pathology ; Heart Conduction System/*pathology ; Humans ; Thanatology ; }, abstract = {The present literature review is focused on the contribution of various pathological changes in the heart conducting system to the tanatogenesis of sudden death from alcoholic cardiomyopathy and coronary heart disease viewed from the perspective of a forensic medical expert. The currently available data on the disorders in the heart conducting system in the subjects with these diseases are presented. Various aspects of pathology of the heart conducting system are considered in the modern and historical contexts. The prospects for the further investigations into the tanatogenic mechanisms of sudden death by reason of alcoholic cardiomyopathy and coronary heart disease are outlined.}, }
@article {pmid22686058, year = {2012}, author = {Denisova, OP and Kul'bitskiĭ, BN and Putintsev, VA and Bogomolova, IN and Bogomolov, DV}, title = {[Characteristics of morphological manifestations of anaphylactic shock in response to the administration of pharmaceutical preparations encountered in the practice of forensic medical expert-histologist].}, journal = {Sudebno-meditsinskaia ekspertiza}, volume = {55}, number = {2}, pages = {46-49}, pmid = {22686058}, issn = {0039-4521}, mesh = {Adult ; Anaphylaxis/chemically induced/*pathology ; *Drug-Related Side Effects and Adverse Reactions ; *Expert Testimony ; Fatal Outcome ; Female ; *Forensic Pathology ; *Forensic Toxicology ; Government Agencies ; *Histology ; Humans ; Male ; Pharmaceutical Preparations/administration &amp; dosage/*classification ; Pregnancy ; Young Adult ; }, abstract = {The authors report the results of a forensic medical investigation of 6 cases of death associated with the administration of pharmaceutical products documented by forensic medical experts of the Russian Centre of Forensic Medical Expertise. The results of the study are compared with the clinical data and summarized using the methods of tanatogenetic analysis. The following main clinical variants of iatrogenic anaphylactic shock (IAS) are distinguished: bronchospastic IAS (n = 1), asphyxic IAS (n = 1), hemodynamic IAS (n = 3), and combined (bronchospastic plus hemodynamic) IAS (n = 1). The signs of all these variants are described allowing for their diagnostics and differentiation diagnostics. These data can be used for the purpose of forensic medical diagnostics and elucidation of the mechanisms of tanatogenesis.}, }
@article {pmid22677281, year = {2012}, author = {Agoni, L and Golden, A and Guha, C and Lenz, J}, title = {Neandertal and Denisovan retroviruses.}, journal = {Current biology : CB}, volume = {22}, number = {11}, pages = {R437-8}, doi = {10.1016/j.cub.2012.04.049}, pmid = {22677281}, issn = {1879-0445}, support = {EB009040/EB/NIBIB NIH HHS/United States ; }, mesh = {Animals ; Base Sequence ; Humans ; Molecular Sequence Data ; Neanderthals/*genetics/virology ; Retroviridae/*genetics ; }, }
@article {pmid22665810, year = {2012}, author = {Wang, X and Mitra, N and Secundino, I and Banda, K and Cruz, P and Padler-Karavani, V and Verhagen, A and Reid, C and Lari, M and Rizzi, E and Balsamo, C and Corti, G and De Bellis, G and Longo, L and ,  and Beggs, W and Caramelli, D and Tishkoff, SA and Hayakawa, T and Green, ED and Mullikin, JC and Nizet, V and Bui, J and Varki, A}, title = {Specific inactivation of two immunomodulatory SIGLEC genes during human evolution.}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {109}, number = {25}, pages = {9935-9940}, pmid = {22665810}, issn = {1091-6490}, support = {DP1 ES022577/ES/NIEHS NIH HHS/United States ; P01 HL107150/HL/NHLBI NIH HHS/United States ; R01 HD051796/HD/NICHD NIH HHS/United States ; }, mesh = {Animals ; *Evolution, Molecular ; Gene Deletion ; *Gene Silencing ; Humans ; Immune System ; Lectins/*genetics ; Primates ; Sialic Acid Binding Immunoglobulin-like Lectins ; }, abstract = {Sialic acid-recognizing Ig-like lectins (Siglecs) are signaling receptors that modulate immune responses, and are targeted for interactions by certain pathogens. We describe two primate Siglecs that were rendered nonfunctional by single genetic events during hominin evolution after our common ancestor with the chimpanzee. SIGLEC13 was deleted by an Alu-mediated recombination event, and a single base pair deletion disrupted the ORF of SIGLEC17. Siglec-13 is expressed on chimpanzee monocytes, innate immune cells that react to bacteria. The human SIGLEC17P pseudogene mRNA is still expressed at high levels in human natural killer cells, which bridge innate and adaptive immune responses. As both resulting pseudogenes are homozygous in all human populations, we resurrected the originally encoded proteins and examined their functions. Chimpanzee Siglec-13 and the resurrected human Siglec-17 recruit a signaling adapter and bind sialic acids. Expression of either Siglec in innate immune cells alters inflammatory cytokine secretion in response to Toll-like receptor-4 stimulation. Both Siglecs can also be engaged by two potentially lethal sialylated bacterial pathogens of newborns and infants, agents with a potential impact on reproductive fitness. Neanderthal and Denisovan genomes show human-like sequences at both loci, corroborating estimates that the initial pseudogenization events occurred in the common ancestral population of these hominins. Both loci also show limited polymorphic diversity, suggesting selection forces predating the origin of modern humans. Taken together, these data suggest that genetic elimination of Siglec-13 and/or Siglec-17 represents signatures of infectious and/or other inflammatory selective processes contributing to population restrictions during hominin origins.}, }
@article {pmid22412940, year = {2012}, author = {Burbano, HA and Green, RE and Maricic, T and Lalueza-Fox, C and de la Rasilla, M and Rosas, A and Kelso, J and Pollard, KS and Lachmann, M and Pääbo, S}, title = {Analysis of human accelerated DNA regions using archaic hominin genomes.}, journal = {PloS one}, volume = {7}, number = {3}, pages = {e32877}, pmid = {22412940}, issn = {1932-6203}, support = {R01 GM082901/GM/NIGMS NIH HHS/United States ; GM082901/GM/NIGMS NIH HHS/United States ; }, mesh = {Adaptation, Biological/genetics ; Animals ; Cluster Analysis ; DNA/chemistry ; Evolution, Molecular ; *Genome ; *Genome, Human ; Hominidae/*genetics ; Humans ; Male ; Mutation ; Neanderthals/genetics ; }, abstract = {Several previous comparisons of the human genome with other primate and vertebrate genomes identified genomic regions that are highly conserved in vertebrate evolution but fast-evolving on the human lineage. These human accelerated regions (HARs) may be regions of past adaptive evolution in humans. Alternatively, they may be the result of non-adaptive processes, such as biased gene conversion. We captured and sequenced DNA from a collection of previously published HARs using DNA from an Iberian Neandertal. Combining these new data with shotgun sequence from the Neandertal and Denisova draft genomes, we determine at least one archaic hominin allele for 84% of all positions within HARs. We find that 8% of HAR substitutions are not observed in the archaic hominins and are thus recent in the sense that the derived allele had not come to fixation in the common ancestor of modern humans and archaic hominins. Further, we find that recent substitutions in HARs tend to have come to fixation faster than substitutions elsewhere in the genome and that substitutions in HARs tend to cluster in time, consistent with an episodic rather than a clock-like process underlying HAR evolution. Our catalog of sequence changes in HARs will help prioritize them for functional studies of genomic elements potentially responsible for modern human adaptations.}, }
@article {pmid22393781, year = {2011}, author = {Maliarchuk, BA}, title = {[Adaptive evolution of the Homo mitochondrial genome].}, journal = {Molekuliarnaia biologiia}, volume = {45}, number = {5}, pages = {845-850}, pmid = {22393781}, issn = {0026-8984}, mesh = {Adaptation, Biological/*genetics ; Amino Acid Sequence ; Amino Acid Substitution/*genetics ; Animals ; *Biological Evolution ; DNA, Mitochondrial/genetics ; Electron Transport Complex IV/*genetics/metabolism ; Genes, Mitochondrial ; *Genome, Mitochondrial ; Hominidae/*genetics ; Humans ; Molecular Sequence Data ; Neanderthals/*genetics ; Phylogeny ; Sequence Homology, Amino Acid ; }, abstract = {Adaptive evolution of 12 protein-coding mitochondrial genes in members of genus Homo (Denisova hominin (H. sp. Altai), Neandertals (H. neanderthalensis) and modern humans (H. sapiens)) has been evaluated by assessing the pattern of changes in the physicochemical properties of amino acid replacements during the primate evolution. It has been found that in the Homo molecular adaptation (positive destabilizing selection) become apparent in the form of 12 radical amino acid replacements accompanied by statistically significant (P < 0.001) changes of physicochemical properties that probably had the functional consequences. These replacements have occurred on the stage of a common ancestor of the Homo (in CO2 and CytB genes) as well as with the appearance of the common ancestor of Neandertals and modern humans (in CO1 and ND5 genes). Radical amino acid replacements were mainly revealed in the cytochrome c oxidase complex IV and cytochrome bc1 complex III, thus coinciding with general trend of increasing of non-synonymous changes in mtDNA genes coding subunits of complexes III and IV proteins in anthropoid primates.}, }
@article {pmid22363811, year = {2012}, author = {Derenko, M and Malyarchuk, B and Denisova, G and Perkova, M and Rogalla, U and Grzybowski, T and Khusnutdinova, E and Dambueva, I and Zakharov, I}, title = {Complete mitochondrial DNA analysis of eastern Eurasian haplogroups rarely found in populations of northern Asia and eastern Europe.}, journal = {PloS one}, volume = {7}, number = {2}, pages = {e32179}, pmid = {22363811}, issn = {1932-6203}, mesh = {Asia ; Asian Continental Ancestry Group/*genetics ; DNA, Mitochondrial/*genetics ; Ethnic Groups/*genetics ; Europe ; European Continental Ancestry Group/*genetics ; Genetic Variation ; *Genetics, Population ; Haplotypes/*genetics ; Humans ; Molecular Sequence Data ; Phylogeny ; Phylogeography ; Principal Component Analysis ; }, abstract = {With the aim of uncovering all of the most basal variation in the northern Asian mitochondrial DNA (mtDNA) haplogroups, we have analyzed mtDNA control region and coding region sequence variation in 98 Altaian Kazakhs from southern Siberia and 149 Barghuts from Inner Mongolia, China. Both populations exhibit the prevalence of eastern Eurasian lineages accounting for 91.9% in Barghuts and 60.2% in Altaian Kazakhs. The strong affinity of Altaian Kazakhs and populations of northern and central Asia has been revealed, reflecting both influences of central Asian inhabitants and essential genetic interaction with the Altai region indigenous populations. Statistical analyses data demonstrate a close positioning of all Mongolic-speaking populations (Mongolians, Buryats, Khamnigans, Kalmyks as well as Barghuts studied here) and Turkic-speaking Sojots, thus suggesting their origin from a common maternal ancestral gene pool. In order to achieve a thorough coverage of DNA lineages revealed in the northern Asian matrilineal gene pool, we have completely sequenced the mtDNA of 55 samples representing haplogroups R11b, B4, B5, F2, M9, M10, M11, M13, N9a and R9c1, which were pinpointed from a massive collection (over 5000 individuals) of northern and eastern Asian, as well as European control region mtDNA sequences. Applying the newly updated mtDNA tree to the previously reported northern Asian and eastern Asian mtDNA data sets has resolved the status of the poorly classified mtDNA types and allowed us to obtain the coalescence age estimates of the nodes of interest using different calibrated rates. Our findings confirm our previous conclusion that northern Asian maternal gene pool consists of predominantly post-LGM components of eastern Asian ancestry, though some genetic lineages may have a pre-LGM/LGM origin.}, }
@article {pmid22343905, year = {2012}, author = {Xu, C and Schmidt, WU and Galinovic, I and Villringer, K and Hotter, B and Ostwaldt, AC and Denisova, N and Kellner, E and Kiselev, V and Fiebach, JB}, title = {The potential of microvessel density in prediction of infarct growth: a two-month experimental study in vessel size imaging.}, journal = {Cerebrovascular diseases (Basel, Switzerland)}, volume = {33}, number = {4}, pages = {303-309}, doi = {10.1159/000335302}, pmid = {22343905}, issn = {1421-9786}, mesh = {Aged ; Aged, 80 and over ; Blood Circulation Time ; Cerebral Infarction/*pathology ; Cohort Studies ; Data Interpretation, Statistical ; Diffusion Magnetic Resonance Imaging ; Disease Progression ; Female ; Follow-Up Studies ; Humans ; Image Processing, Computer-Assisted ; Magnetic Resonance Angiography ; Male ; Microvessels/*pathology ; Middle Aged ; Predictive Value of Tests ; ROC Curve ; Reperfusion Injury/pathology ; Tissue Plasminogen Activator/therapeutic use ; }, abstract = {OBJECTIVES: Vessel size imaging is a novel technique to evaluate pathological changes of the microvessel density quantity Q and the mean vessel size index (VSI). As a follow-up study, we assessed these parameters for microscopic description of ischemic penumbra and their potentials in predicting lesion growth.<br><br>METHODS: Seventy-five patients with a perfusion-diffusion mismatch were examined within 24 h from symptom onset. We defined three regions of interest: the initial infarct (INF), the ischemic penumbra (IPE), and the healthy region (HEA) symmetric to the IPE. For 23 patients with a 6th-day follow-up, IPE regions were divided into areas of infarct growth and areas of oligemia.<br><br>RESULT: The median values of Q and VSI were: for INF 0.29 s(-1/3) and 15.8 μm, for IPE 0.33 s(-1/3) and 20.6 μm and for HEA 0.36 s(-1/3) and 17.4 μm. The Q in the IPE was significantly smaller than in HEA, and VSI was significantly larger. The Q with a threshold of 0.32 s(-1/3) predicted the final infarction with a sensitivity of 69% and a specificity of 64%.<br><br>CONCLUSIONS: The reduced Q and increased VSI in the IPE confirmed our previous pilot results. Although Q showed a trend to identify the severity of ischemia in an overall voxel population, its potential in predicting infarct growth needs to be further tested in a larger cohort including a clear status of reperfusion and recanalization.}, }
@article {pmid22334930, year = {2011}, author = {Denisova, OA and Titarova, LS}, title = {[The atypical course of subperiosteal abscess of the orbital wall].}, journal = {Vestnik otorinolaringologii}, volume = {}, number = {5}, pages = {66-67}, pmid = {22334930}, issn = {0042-4668}, mesh = {Abscess/*pathology ; Anti-Bacterial Agents/administration &amp; dosage ; Child ; Endoscopy/*methods ; Female ; Fibrinolytic Agents/administration &amp; dosage ; Humans ; Leeching/methods ; *Maxillary Sinusitis/diagnostic imaging/etiology/physiopathology ; Nasal Bone/abnormalities ; *Nasal Obstruction/complications/congenital/diagnosis/physiopathology ; Orbit/*pathology ; *Orbital Diseases/diagnosis/etiology/physiopathology/therapy ; Punctures ; Radiography ; Rhinoplasty/*methods ; Treatment Outcome ; Turbinates/abnormalities ; }, abstract = {The authors describe a clinical case of malformation of intranasal structures of the lateral wall and the middle turbinated bone of the nose (bullous middle turbinated bone, lateral position of the middle turbinated bone, hypertrophy of ethmoidal bulla and uncinate process) in combination with expressed deformation of the nasal septum. These malformations were responsible for the development of acute hemisinusitis complicated by subperiosteal abscess of the superomedial orbital wall. Conjunctival chemosis was impossible to remove by traditional medicamental therapy and surgical intervention. Hirudotherapy produced the well-apparent anti-odematous, anti-inflammatory, anti-coagulative, and thrombolytic effects that resulted in reduced conjunctival oedema and marked positive dynamics of the state of the eyeball. Subcutaneous administration of anticoagulants was used to prevent thrombosis of orbital veins and cerebral venous sinuses.}, }
@article {pmid22319157, year = {2012}, author = {Mendez, FL and Watkins, JC and Hammer, MF}, title = {Global genetic variation at OAS1 provides evidence of archaic admixture in Melanesian populations.}, journal = {Molecular biology and evolution}, volume = {29}, number = {6}, pages = {1513-1520}, doi = {10.1093/molbev/msr301}, pmid = {22319157}, issn = {1537-1719}, mesh = {2',5'-Oligoadenylate Synthetase/*genetics ; 5' Untranslated Regions/genetics ; African Continental Ancestry Group/*genetics ; Animals ; Asian Continental Ancestry Group/genetics ; Chromosomes, Human, Pair 12/genetics ; Genetic Speciation ; Genotyping Techniques ; Haplotypes ; Hominidae/genetics ; Humans ; *Hybridization, Genetic ; Linkage Disequilibrium ; Melanesia ; Models, Genetic ; Oceanic Ancestry Group/genetics ; *Polymorphism, Genetic ; Sequence Analysis, DNA ; }, abstract = {Recent analysis of DNA extracted from two Eurasian forms of archaic human shows that more genetic variants are shared with humans currently living in Eurasia than with anatomically modern humans in sub-Saharan Africa. Although these genome-wide average measures of genetic similarity are consistent with the hypothesis of archaic admixture in Eurasia, analyses of individual loci exhibiting the signal of archaic introgression are needed to test alternative hypotheses and investigate the admixture process. Here, we provide a detailed sequence analysis of the innate immune gene OAS1, a locus with a divergent Melanesian haplotype that is very similar to the Denisova sequence from the Altai region of Siberia. We resequenced a 7-kb region encompassing the OAS1 gene in 88 individuals from six Old World populations (San, Biaka, Mandenka, French Basque, Han Chinese, and Papua New Guineans) and discovered previously unknown and ancient genetic variation. The 5' region of this gene has unusual patterns of diversity, including 1) higher levels of nucleotide diversity in Papuans than in sub-Saharan Africans, 2) very deep ancestry with an estimated time to the most recent common ancestor of >3 myr, and 3) a basal branching pattern with Papuan individuals on either side of the rooted network. A global geographic survey of >1,500 individuals showed that the divergent Papuan haplotype is nearly restricted to populations from eastern Indonesia and Melanesia. Polymorphic sites within this haplotype are shared with the draft Denisova genome over a span of ∼90 kb and are associated with an extended block of linkage disequilibrium, supporting the hypothesis that this haplotype introgressed from an archaic source that likely lived in Eurasia.}, }
@article {pmid22238107, year = {2012}, author = {Wang, Y and Song, JH and Denisova, JV and Park, WM and Fontes, JD and Belousov, AB}, title = {Neuronal gap junction coupling is regulated by glutamate and plays critical role in cell death during neuronal injury.}, journal = {The Journal of neuroscience : the official journal of the Society for Neuroscience}, volume = {32}, number = {2}, pages = {713-725}, pmid = {22238107}, issn = {1529-2401}, support = {R01 NS064256/NS/NINDS NIH HHS/United States ; R01 NS064256-02/NS/NINDS NIH HHS/United States ; HD002528/HD/NICHD NIH HHS/United States ; R01 NS064256-01A1/NS/NINDS NIH HHS/United States ; P30 HD002528/HD/NICHD NIH HHS/United States ; }, mesh = {Animals ; Brain Ischemia/*pathology/physiopathology ; Cell Death/physiology ; Cells, Cultured ; Cerebral Cortex/cytology ; Connexins/genetics/physiology ; Gap Junctions/*physiology ; Glutamic Acid/*physiology ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Neurons/pathology/*physiology ; }, abstract = {In the mammalian CNS, excessive release of glutamate and overactivation of glutamate receptors are responsible for the secondary (delayed) neuronal death following neuronal injury, including ischemia, traumatic brain injury (TBI), and epilepsy. The coupling of neurons by gap junctions (electrical synapses) increases during neuronal injury. We report here that the ischemic increase in neuronal gap junction coupling is regulated by glutamate via group II metabotropic glutamate receptors (mGluRs). Specifically, using electrotonic coupling, Western blots, and siRNA in the mouse somatosensory cortex in vivo and in vitro, we demonstrate that activation of group II mGluRs increases background levels of neuronal gap junction coupling and expression of connexin 36 (Cx36) (neuronal gap junction protein), and inactivation of group II mGluRs prevents the ischemia-mediated increases in the coupling and Cx36 expression. We also show that the regulation is via cAMP/PKA (cAMP-dependent protein kinase)-dependent signaling and posttranscriptional control of Cx36 expression and that other glutamate receptors are not involved in these regulatory mechanisms. Furthermore, using the analysis of neuronal death, we show that inactivation of group II mGluRs or genetic elimination of Cx36 both dramatically reduce ischemia-mediated neuronal death in vitro and in vivo. Similar results are obtained using in vitro models of TBI and epilepsy. Our results indicate that neuronal gap junction coupling is a critical component of glutamate-dependent neuronal death. They also suggest that causal link among group II mGluR function, neuronal gap junction coupling, and neuronal death has a universal character and operates in different types of neuronal injuries.}, }
@article {pmid22224240, year = {2011}, author = {Morgunov, LIu and Denisova, IA and Rozhkova, TI and Stakhovskaia, LV and Skvortsova, VI}, title = {[Androgenic deficit and its treatment in stroke male patients with diabetes mellitus type II].}, journal = {Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova}, volume = {111}, number = {8 Pt 2}, pages = {21-24}, pmid = {22224240}, issn = {1997-7298}, mesh = {Aged ; Androgens/*deficiency ; Diabetes Mellitus, Type 2/*metabolism ; *Hormone Replacement Therapy ; Humans ; Male ; Middle Aged ; Stroke/*metabolism ; Testosterone/administration &amp; dosage/*analogs &amp; derivatives ; }, abstract = {We have studied 154 men (mean age 61,4+/-4,1 years) with the first hemispheric ischemic stroke. Clinical and laboratory studies have revealed the androgenic deficit in 66,3%, with its frequency higher in patients with diabetes mellitus type II (50 and 26,3%, respectively). Forty-two men with diabetes mellitus type II and acquired androgenic deficit received replacing treatment with testosterone undecanoate in dose 1000 mg intramuscular. The treatment was started one week after the development of stroke, the next injections were given after 6 weeks and then every 12 weeks during 2 years. The control group included 30 males who did not receive androgens. After 2 years from the beginning of treatment, there were the decrease in clinical severity of androgenic deficit, the increase of total and free testosterone levels, and muscle power in the main group compared to the controls. Body mass index, glicated hemoglobin, cholesterol, triglycerides, low density lipoproteins have decreased as well. Secondary stroke has developed in 3 (7,1%) patients of the main group and in 5 (16,6%) controls; 12 (28,6%) patients returned to work in the main group compared to 2 (6,6%) in the control group. The treatment with androgens has a positive effect on risk factors of secondary ischemic stroke. It is an effective method for improvement of social adaptation of men survived after the stroke.}, }
@article {pmid22192823, year = {2011}, author = {Lalueza-Fox, C and Gilbert, MT}, title = {Paleogenomics of archaic hominins.}, journal = {Current biology : CB}, volume = {21}, number = {24}, pages = {R1002-9}, doi = {10.1016/j.cub.2011.11.021}, pmid = {22192823}, issn = {1879-0445}, mesh = {Animals ; *Biological Evolution ; Cell Nucleus/genetics ; DNA, Mitochondrial/genetics ; Demography ; *Evolution, Molecular ; Fossils ; Gene Flow ; Genetic Variation ; Genome, Human ; Hominidae/anatomy &amp; histology/*genetics/physiology ; Humans/anatomy &amp; histology/genetics/physiology ; Neanderthals/anatomy &amp; histology/*genetics/physiology ; Phylogeny ; Species Specificity ; }, abstract = {In order to understand the genetic basis for the evolutionary success of modern humans, it is necessary to compare their genetic makeup to that of closely related species. Unfortunately, our closest living relatives, the chimpanzees, are evolutionarily quite distant. With the advent of ancient DNA study and more recently paleogenomics - the study of the genomes of ancient organisms - it has become possible to compare human genomes to those of much more closely related groups. Our closest known relatives are the Neanderthals, which evolved and lived in Europe and Western Asia, from about 600,000 years ago until their disappearance around 30,000 years ago following the expansion of anatomically modern humans into their range. The closely related Denisovans are only known by virtue of their DNA, which has been extracted from bone fragments dating around 30,000 to 50,000 years ago found in a single Siberian cave. Analyses of Neanderthal and Denisovan nuclear and mitochondrial genomes have revealed surprising insights into these archaic humans as well as our own species. The genomes provide a preliminary catalogue of derived amino acids that are specific to all extant modern humans, thus offering insights into the functional differences between the three lineages. In addition, the genomes provide evidence of gene flow between the three lineages after anatomically modern humans left Africa, drastically changing our view of human evolution.}, }
@article {pmid22042846, year = {2011}, author = {Skoglund, P and Jakobsson, M}, title = {Archaic human ancestry in East Asia.}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {108}, number = {45}, pages = {18301-18306}, pmid = {22042846}, issn = {1091-6490}, mesh = {Asia ; *Fossils ; Genetic Drift ; Genotype ; History, Ancient ; Humans ; Principal Component Analysis ; }, abstract = {Recent studies of ancient genomes have suggested that gene flow from archaic hominin groups to the ancestors of modern humans occurred on two separate occasions during the modern human expansion out of Africa. At the same time, decreasing levels of human genetic diversity have been found at increasing distance from Africa as a consequence of human expansion out of Africa. We analyzed the signal of archaic ancestry in modern human populations, and we investigated how serial founder models of human expansion affect the signal of archaic ancestry using simulations. For descendants of an archaic admixture event, we show that genetic drift coupled with ascertainment bias for common alleles can cause artificial but largely predictable differences in similarity to archaic genomes. In genotype data from non-Africans, this effect results in a biased genetic similarity to Neandertals with increasing distance from Africa. However, in addition to the previously reported gene flow between Neandertals and non-Africans as well as gene flow between an archaic human population from Siberia ("Denisovans") and Oceanians, we found a significant affinity between East Asians, particularly Southeast Asians, and the Denisova genome--a pattern that is not expected under a model of solely Neandertal admixture in the ancestry of East Asians. These results suggest admixture between Denisovans or a Denisova-related population and the ancestors of East Asians, and that the history of anatomically modern and archaic humans might be more complex than previously proposed.}, }
@article {pmid21944045, year = {2011}, author = {Reich, D and Patterson, N and Kircher, M and Delfin, F and Nandineni, MR and Pugach, I and Ko, AM and Ko, YC and Jinam, TA and Phipps, ME and Saitou, N and Wollstein, A and Kayser, M and Pääbo, S and Stoneking, M}, title = {Denisova admixture and the first modern human dispersals into Southeast Asia and Oceania.}, journal = {American journal of human genetics}, volume = {89}, number = {4}, pages = {516-528}, pmid = {21944045}, issn = {1537-6605}, mesh = {Animals ; Asia, Southeastern ; Biological Evolution ; DNA, Mitochondrial/genetics ; *Gene Flow ; Genotype ; Geography ; Hominidae/*genetics ; Humans ; Models, Genetic ; Models, Statistical ; Oceania ; Oceanic Ancestry Group ; Pan troglodytes ; Polymorphism, Single Nucleotide ; }, abstract = {It has recently been shown that ancestors of New Guineans and Bougainville Islanders have inherited a proportion of their ancestry from Denisovans, an archaic hominin group from Siberia. However, only a sparse sampling of populations from Southeast Asia and Oceania were analyzed. Here, we quantify Denisova admixture in 33 additional populations from Asia and Oceania. Aboriginal Australians, Near Oceanians, Polynesians, Fijians, east Indonesians, and Mamanwa (a "Negrito" group from the Philippines) have all inherited genetic material from Denisovans, but mainland East Asians, western Indonesians, Jehai (a Negrito group from Malaysia), and Onge (a Negrito group from the Andaman Islands) have not. These results indicate that Denisova gene flow occurred into the common ancestors of New Guineans, Australians, and Mamanwa but not into the ancestors of the Jehai and Onge and suggest that relatives of present-day East Asians were not in Southeast Asia when the Denisova gene flow occurred. Our finding that descendants of the earliest inhabitants of Southeast Asia do not all harbor Denisova admixture is inconsistent with a history in which the Denisova interbreeding occurred in mainland Asia and then spread over Southeast Asia, leading to all its earliest modern human inhabitants. Instead, the data can be most parsimoniously explained if the Denisova gene flow occurred in Southeast Asia itself. Thus, archaic Denisovans must have lived over an extraordinarily broad geographic and ecological range, from Siberia to tropical Asia.}, }
@article {pmid21897868, year = {2011}, author = {Vorobieva, NV and Sherbakov, DY and Druzhkova, AS and Stanyon, R and Tsybankov, AA and Vasil'ev, SK and Shunkov, MV and Trifonov, VA and Graphodatsky, AS}, title = {Genotyping of Capreolus pygargus fossil DNA from Denisova cave reveals phylogenetic relationships between ancient and modern populations.}, journal = {PloS one}, volume = {6}, number = {8}, pages = {e24045}, pmid = {21897868}, issn = {1932-6203}, mesh = {Animals ; DNA/*genetics/isolation &amp; purification ; Deer/*genetics ; *Evolution, Molecular ; *Fossils ; Genetic Variation ; *Genotyping Techniques ; Geology ; Haplotypes ; *Phylogeny ; Sequence Analysis, DNA ; Siberia ; }, abstract = {BACKGROUND: The extant roe deer (Capreolus Gray, 1821) includes two species: the European roe deer (C. capreolus) and the Siberian roe deer (C. pygargus) that are distinguished by morphological and karyotypical differences. The Siberian roe deer occupies a vast area of Asia and is considerably less studied than the European roe deer. Modern systematics of the Siberian roe deer remain controversial with 4 morphological subspecies. Roe deer fossilized bones are quite abundant in Denisova cave (Altai Mountains, South Siberia), where dozens of both extant and extinct mammalian species from modern Holocene to Middle Pleistocene have been retrieved.<br><br>We analyzed a 629 bp fragment of the mitochondrial control region from ancient bones of 10 Holocene and four Pleistocene Siberian roe deer from Denisova cave as well as 37 modern specimen belonging to populations from Altai, Tian Shan (Kyrgyzstan), Yakutia, Novosibirsk region and the Russian Far East. Genealogical reconstructions indicated that most Holocene haplotypes were probably ancestral for modern roe deer populations of Western Siberia and Tian Shan. One of the Pleistocene haplotypes was possibly ancestral for modern Yakutian populations, and two extinct Pleistocene haplotypes were close to modern roe deer from Tian Shan and Yakutia. Most modern geographical populations (except for West Siberian Plains) are heterogeneous and there is some tentative evidence for structure. However, we did not find any distinct phylogenetic signal characterizing particular subspecies in either modern or ancient samples.<br><br>CONCLUSION/SIGNIFICANCE: Analysis of mitochondrial DNA from both ancient and modern samples of Siberian roe deer shed new light on understanding the evolutionary history of roe deer. Our data indicate that during the last 50,000 years multiple replacements of populations of the Siberian roe deer took place in the Altai Mountains correlating with climatic changes. The Siberian roe deer represent a complex and heterogeneous species with high migration rates and without evident subspecies structure. Low genetic diversity of the West Siberian Plain population indicates a recent bottleneck or founder effect.}, }
@article {pmid21868647, year = {2011}, author = {Gibbons, A}, title = {Paleoanthropology. A Denisovan legacy in the immune system?.}, journal = {Science (New York, N.Y.)}, volume = {333}, number = {6046}, pages = {1086}, doi = {10.1126/science.333.6046.1086}, pmid = {21868647}, issn = {1095-9203}, mesh = {Alleles ; Animals ; Asian Continental Ancestry Group/genetics ; European Continental Ancestry Group/genetics ; Female ; *Fossils ; *Genes, MHC Class I ; HLA-B Antigens/*genetics ; HLA-C Antigens/*genetics ; Hominidae/*genetics/*immunology ; Humans ; }, }
@article {pmid21868630, year = {2011}, author = {Abi-Rached, L and Jobin, MJ and Kulkarni, S and McWhinnie, A and Dalva, K and Gragert, L and Babrzadeh, F and Gharizadeh, B and Luo, M and Plummer, FA and Kimani, J and Carrington, M and Middleton, D and Rajalingam, R and Beksac, M and Marsh, SG and Maiers, M and Guethlein, LA and Tavoularis, S and Little, AM and Green, RE and Norman, PJ and Parham, P}, title = {The shaping of modern human immune systems by multiregional admixture with archaic humans.}, journal = {Science (New York, N.Y.)}, volume = {334}, number = {6052}, pages = {89-94}, pmid = {21868630}, issn = {1095-9203}, support = {R01 AI031168/AI/NIAID NIH HHS/United States ; AI031168/AI/NIAID NIH HHS/United States ; RR000165/RR/NCRR NIH HHS/United States ; P51 RR000165/RR/NCRR NIH HHS/United States ; //Intramural NIH HHS/United States ; HHSN261200800001E//PHS HHS/United States ; HHSN261200800001C/RC/CCR NIH HHS/United States ; HHSN261200800001E/CA/NCI NIH HHS/United States ; }, mesh = {Adaptation, Biological ; African Continental Ancestry Group/genetics ; Alleles ; Animals ; Asian Continental Ancestry Group/genetics ; Continental Population Groups/*genetics ; European Continental Ancestry Group/genetics ; Evolution, Molecular ; *Genes, MHC Class I ; Genetic Variation ; HLA-A Antigens/*genetics/immunology/metabolism ; HLA-B Antigens/*genetics/immunology/metabolism ; HLA-C Antigens/*genetics/immunology ; Haplotypes ; Hominidae/*genetics/*immunology ; Humans ; *Hybridization, Genetic ; Killer Cells, Natural/immunology ; Ligands ; Linkage Disequilibrium ; Molecular Sequence Data ; Oceanic Ancestry Group/genetics ; Receptors, KIR/immunology/metabolism ; Selection, Genetic ; }, abstract = {Whole genome comparisons identified introgression from archaic to modern humans. Our analysis of highly polymorphic human leukocyte antigen (HLA) class I, vital immune system components subject to strong balancing selection, shows how modern humans acquired the HLA-B*73 allele in west Asia through admixture with archaic humans called Denisovans, a likely sister group to the Neandertals. Virtual genotyping of Denisovan and Neandertal genomes identified archaic HLA haplotypes carrying functionally distinctive alleles that have introgressed into modern Eurasian and Oceanian populations. These alleles, of which several encode unique or strong ligands for natural killer cell receptors, now represent more than half the HLA alleles of modern Eurasians and also appear to have been later introduced into Africans. Thus, adaptive introgression of archaic alleles has significantly shaped modern human immune systems.}, }
@article {pmid21807602, year = {2011}, author = {Zhang, G and Pei, Z and Ball, EV and Mort, M and Kehrer-Sawatzki, H and Cooper, DN}, title = {Cross-comparison of the genome sequences from human, chimpanzee, Neanderthal and a Denisovan hominin identifies novel potentially compensated mutations.}, journal = {Human genomics}, volume = {5}, number = {5}, pages = {453-484}, pmid = {21807602}, issn = {1479-7364}, mesh = {Animals ; Databases, Genetic ; *Genome ; Genome, Human ; Hominidae/*genetics ; Humans ; *Mutation ; Neanderthals/genetics ; Pan troglodytes/*genetics ; }, abstract = {The recent publication of the draft genome sequences of the Neanderthal and a ∼50,000-year-old archaic hominin from Denisova Cave in southern Siberia has ushered in a new age in molecular archaeology. We previously cross-compared the human, chimpanzee and Neanderthal genome sequences with respect to a set of disease-causing/disease-associated missense and regulatory mutations (Human Gene Mutation Database) and succeeded in identifying genetic variants which, although apparently pathogenic in humans, may represent a 'compensated' wild-type state in at least one of the other two species. Here, in an attempt to identify further 'potentially compensated mutations' (PCMs) of interest, we have compared our dataset of disease-causing/disease-associated mutations with their corresponding nucleotide positions in the Denisovan hominin, Neanderthal and chimpanzee genomes. Of the 15 human putatively disease-causing mutations that were found to be compensated in chimpanzee, Denisovan or Neanderthal, only a solitary F5 variant (Val1736Met) was specific to the Denisovan. In humans, this missense mutation is associated with activated protein C resistance and an increased risk of thromboembolism and recurrent miscarriage. It is unclear at this juncture whether this variant was indeed a PCM in the Denisovan or whether it could instead have been associated with disease in this ancient hominin.}, }
@article {pmid21803765, year = {2011}, author = {Crisci, JL and Wong, A and Good, JM and Jensen, JD}, title = {On characterizing adaptive events unique to modern humans.}, journal = {Genome biology and evolution}, volume = {3}, number = {}, pages = {791-798}, pmid = {21803765}, issn = {1759-6653}, mesh = {Adaptation, Biological/genetics ; Amino Acid Substitution/*genetics ; Animals ; Biological Evolution ; Genome, Human/*genetics ; Hominidae/*genetics ; Humans ; Neanderthals/*genetics ; Pan troglodytes/genetics ; Selection, Genetic ; }, abstract = {Ever since the first draft of the human genome was completed in 2001, there has been increased interest in identifying genetic changes that are uniquely human, which could account for our distinct morphological and cognitive capabilities with respect to other apes. Recently, draft sequences of two extinct hominin genomes, a Neanderthal and Denisovan, have been released. These two genomes provide a much greater resolution to identify human-specific genetic differences than the chimpanzee, our closest extant relative. The Neanderthal genome paper presented a list of regions putatively targeted by positive selection around the time of the human-Neanderthal split. We here seek to characterize the evolutionary history of these candidate regions-examining evidence for selective sweeps in modern human populations as well as for accelerated adaptive evolution across apes. Results indicate that 3 of the top 20 candidate regions show evidence of selection in at least one modern human population (P < 5 × 10(5)). Additionally, four genes within the top 20 regions show accelerated amino acid substitutions across multiple apes (P < 0.01), suggesting importance across deeper evolutionary time. These results highlight the importance of evaluating evolutionary processes across both recent and ancient evolutionary timescales and intriguingly suggest a list of candidate genes that may have been uniquely important around the time of the human-Neanderthal split.}, }
@article {pmid21796732, year = {2011}, author = {Sauter, D and Vogl, M and Kirchhoff, F}, title = {Ancient origin of a deletion in human BST2/Tetherin that confers protection against viral zoonoses.}, journal = {Human mutation}, volume = {32}, number = {11}, pages = {1243-1245}, doi = {10.1002/humu.21571}, pmid = {21796732}, issn = {1098-1004}, mesh = {Amino Acid Sequence ; Animals ; Antigens, CD/*genetics ; *Evolution, Molecular ; GPI-Linked Proteins/genetics ; Host-Pathogen Interactions ; Humans ; Molecular Sequence Data ; Neanderthals ; Primates ; *Sequence Deletion ; Simian Acquired Immunodeficiency Syndrome/genetics/transmission/virology ; Simian Immunodeficiency Virus/genetics/immunology/pathogenicity ; Zoonoses/*virology ; }, abstract = {Bone marrow stromal antigen 2 (BST2)/Tetherin is an antiviral factor that blocks the release of enveloped virions from infected cells. Recent data suggest that efficient BST2 antagonism was a prerequisite for the global spread of HIV/AIDS. Most simian immunodeficiency viruses (SIVs), including the direct precursors of HIV, use their Nef protein to antagonize BST2 of their respective host species. Human BST2, however, contains a five amino acid deletion in its cytoplasmic domain that confers resistance to Nef. Thus, this antiviral factor constitutes a major barrier to effective zoonotic transmissions of SIVs to humans. Here, we show that this protective deletion has already been present in Neanderthal and Denisovan BST2 and thus evolved at least 800,000 years ago. This ancient origin helps to explain why effectively spreading zoonotic transmissions of SIVs to humans have been rare, although SIVs are widespread in African nonhuman primates and humans must have been exposed to these viruses many times.}, }
@article {pmid21786602, year = {2011}, author = {Baranov, AA and Alekseeva, EI and Valieva, SI and Bzarova, TM and Denisova, RV and Lisitsyn, AO and Chistiakova, EG and Sleptsova, TV and Mitenko, EV}, title = {[Biological therapy in pediatric rheumatology].}, journal = {Vestnik Rossiiskoi akademii meditsinskikh nauk}, volume = {}, number = {6}, pages = {27-36}, pmid = {21786602}, issn = {0869-6047}, mesh = {Adolescent ; Antibodies, Monoclonal/pharmacokinetics/therapeutic use ; *Antirheumatic Agents/pharmacokinetics/therapeutic use ; *Arthritis, Juvenile/metabolism/physiopathology/therapy ; Biological Availability ; Biological Therapy/*methods ; Child ; Child, Preschool ; Etanercept ; Female ; Humans ; Immunoglobulin G/metabolism/therapeutic use ; *Immunosuppressive Agents/pharmacokinetics/therapeutic use ; Interleukin 1 Receptor Antagonist Protein/pharmacokinetics/therapeutic use ; Male ; Quality of Life ; Quality-Adjusted Life Years ; Receptors, Tumor Necrosis Factor/metabolism/therapeutic use ; Remission Induction ; Severity of Illness Index ; Treatment Outcome ; }, abstract = {This article presents the unique experience of Research Centre of Children's Health in the treatment of 438 children with juvenile rheumatoid arthritis using biological preparations, viz. infliximab (270), adalimumab (55), ethanercept (25), rituximab (74), and tocilizumab (34). It is shown that differential biological therapy, unlike treatment with classical immunodepressants (methotrexate, cyclosporine, leflunomide, etc.), permits to achieve clinico-laboratory remission in 70% and markedly decrease activity of the disease in 20% of the patients within 1 year after the onset. It is concluded that treatment with gene-engineered drugs improves the quality of life of the children and their families, normal growth and development of the patients and affects prognosis of this formerly incurable chronic autoimmune disease.}, }
@article {pmid21677663, year = {2011}, author = {Malyarchuk, B and Derenko, M and Denisova, G and Maksimov, A and Wozniak, M and Grzybowski, T and Dambueva, I and Zakharov, I}, title = {Ancient links between Siberians and Native Americans revealed by subtyping the Y chromosome haplogroup Q1a.}, journal = {Journal of human genetics}, volume = {56}, number = {8}, pages = {583-588}, doi = {10.1038/jhg.2011.64}, pmid = {21677663}, issn = {1435-232X}, mesh = {Asia ; Asian Continental Ancestry Group/genetics ; Chromosomes, Human, Y/*genetics ; Gene Frequency ; Genetic Variation ; Genetics, Population/*methods ; Haplotypes/*genetics ; Humans ; Indians, North American/ethnology/genetics ; Male ; Microsatellite Repeats/genetics ; Pedigree ; *Phylogeny ; Polymorphism, Single Nucleotide/genetics ; Siberia/ethnology ; }, abstract = {To investigate the structure of Y chromosome haplogroups R-M207 and Q-M242 in human populations of North Asia, we have performed high-resolution genotyping using both single nucleotide polymorphisms and short tandem repeat (STR)-based approaches of 121 M207- and M242-derived samples from 885 males of 16 ethnic groups of Siberia and East Asia. As a result, the following Y chromosome haplogroups were revealed: R1b1b1-M73 (2.0%), R1b1b2-M269 (0.7%), R2-M124 (1.1%), Q1a*-MEH2 (0.5%), Q1a2-M25 (0.1%), Q1a3*-M346 (9.2%) and Q1a3a-M3 (0.2%). Despite the low coalescence age of haplogroup Q1a3*-M346, which is estimated in South Siberia as about 4.5±1.5 thousand years ago (Ka), divergence time between these Q1a3*-M346 haplotypes and Amerindian-specific haplogroup Q1a3a-M3 is equal to 13.8±3.9 Ka, pointing to a relatively recent entry date to America. In addition, unique cluster of haplotypes belonging to Q1a*-MEH2 was found in Koryaks inhabiting the Sea of Okhotsk coast (at a frequency of 10.3%). Although the level of STR diversity associated with Q1a*-MEH2 is very low, this lineage appears to be closest to the extinct Palaeo-Eskimo individuals belonging to the Saqqaq culture arisen in the New World Arctic about 5.5 Ka. This finding suggests that Q1a*-MEH2 likely traces a population migration originating in Northeast Siberia across the Bering Strait.}, }
@article {pmid21645926, year = {2011}, author = {Denisova, G and Jenkins, K and Evelegh, C and Bramson, J}, title = {Novel method for differentiation between Trastuzumab and host adaptive response.}, journal = {Molecular immunology}, volume = {48}, number = {15-16}, pages = {1882-1885}, doi = {10.1016/j.molimm.2011.05.016}, pmid = {21645926}, issn = {1872-9142}, mesh = {*Adaptive Immunity ; Antibodies, Monoclonal/*blood/therapeutic use ; Antibodies, Monoclonal, Humanized ; Antibody Affinity ; Antineoplastic Agents/blood/therapeutic use ; Autoantibodies/*blood/immunology ; Autoantigens/immunology ; Blotting, Western ; Breast Neoplasms/drug therapy/immunology ; Female ; Hematologic Tests/*methods ; Humans ; Mutation ; Receptor, ErbB-2/genetics/*immunology ; Recombinant Proteins/genetics/immunology ; Trastuzumab ; }, abstract = {Humoral immune response to human epidermal growth factor receptor 2 (HER-2/neu or ErbB-2) has been detected in sera of breast cancer patients and shown to be an appropriate prognostic marker (Taylor et al., 2007). However, since Trastuzumab (Herceptin) is a widely used monoclonal antibody as cancer therapy agent for tumors over-expressing HER-2, there is a need for an efficient way to detect host-generated antibodies against HER-2 without the confounding effect of Herceptin. Here we describe a screening method developed to decipher between host antibodies against HER-2 and that of Herceptin. By producing a series of truncation mutants within the epitope of Herceptin, we were able to inhibit this binding. We demonstrated also that by a three amino acid substitution (PPF→SSS) we were able to abrogate Herceptin binding while generating a highly conserved HER-2 extracellular domain (ECD). By producing a stable cell line that expresses this mutated form of the human HER-2 ECD, we have a source of this protein to probe patient sera. Our method represents a proof of principle that mutated HER-2 which we constructed could be used to distinguish between a host response against HER-2 and the monoclonal antibody Herceptin targeting the same protein.}, }
@article {pmid21542308, year = {2011}, author = {Denisova, GA and Malarchuk, BA and Derenko, MV and Kravtsova, OA}, title = {[Population structure of Volga Tatars inferred from the mitochondrial DNA diversity data].}, journal = {Genetika}, volume = {47}, number = {3}, pages = {387-393}, pmid = {21542308}, issn = {0016-6758}, mesh = {DNA, Mitochondrial/*genetics ; Ethnic Groups/*genetics ; Gene Frequency/genetics ; Genetic Variation ; Haplotypes/genetics ; Humans ; Polymorphism, Restriction Fragment Length/genetics ; Russia/ethnology ; }, abstract = {The data on mitochondrial DNA (mtDNA) variation in two populations of Volga Tatars, representing the population of Buinsk and Aznakaevo districts of the Republic of Tatarstan are presented. Comparative analysis of the data on mtDNA variation in the populations of Eastern Europe showed that Volga Tatars were characterized by low interpopulation differentiation (F(ST) = 0.33%), while the level of interethnic differentiation in Eastern Europe is 1.8%. Genetic similarity of Tatars from the eastern regions of Tatarstan to Bashkirs, as well as of Tatars from western regions to Chuvashes, with whom they share territorial borders, was revealed. Positive correlation between population genetic structure in Eastern Europe and linguistic affiliation of the ethnic groups studied was observed.}, }
@article {pmid21508216, year = {2011}, author = {Park, WM and Wang, Y and Park, S and Denisova, JV and Fontes, JD and Belousov, AB}, title = {Interplay of chemical neurotransmitters regulates developmental increase in electrical synapses.}, journal = {The Journal of neuroscience : the official journal of the Society for Neuroscience}, volume = {31}, number = {16}, pages = {5909-5920}, pmid = {21508216}, issn = {1529-2401}, support = {R01 DA015088/DA/NIDA NIH HHS/United States ; R01 NS064256/NS/NINDS NIH HHS/United States ; P20 RR024214/RR/NCRR NIH HHS/United States ; HD002528/HD/NICHD NIH HHS/United States ; P30 HD002528/HD/NICHD NIH HHS/United States ; }, mesh = {Analysis of Variance ; Animals ; Blotting, Western ; Cell Communication/physiology ; Cells, Cultured ; Cerebral Cortex/embryology/*metabolism ; Connexins/genetics/*metabolism ; Electrical Synapses/*metabolism ; Female ; Hypothalamus/embryology/*metabolism ; Male ; Mice ; Mice, Knockout ; Neurons/*metabolism ; Neurotransmitter Agents/*metabolism ; RNA, Small Interfering ; Rats ; Rats, Sprague-Dawley ; Receptors, Metabotropic Glutamate/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Synaptic Transmission/physiology ; }, abstract = {Coupling of neurons by electrical synapses (gap junctions) transiently increases in the mammalian CNS during development. We report here that the developmental increase in neuronal gap junction coupling and expression of connexin 36 (Cx36; neuronal gap junction protein) are regulated by an interplay between the activity of group II metabotropic glutamate receptors (mGluRs) and GABA(A) receptors. Specifically, using dye coupling, electrotonic coupling, Western blots and small interfering RNA in the rat and mouse hypothalamus and cortex in vivo and in vitro, we demonstrate that activation of group II mGluRs augments, and inactivation prevents, the developmental increase in neuronal gap junction coupling and Cx36 expression. However, changes in GABA(A) receptor activity have the opposite effects. The regulation by group II mGluRs is via cAMP/PKA-dependent signaling, and regulation by GABA(A) receptors is via Ca(2+)/PKC-dependent signaling. Furthermore, the receptor-mediated upregulation of Cx36 requires a neuron-restrictive silencer element in the Cx36 gene promoter, and the downregulation involves the 3'-untranslated region of the Cx36 mRNA, as shown using reverse-transcription quantitative real-time PCR and luciferase reporter activity analysis. In addition, the methyl thiazolyl tetrazolium analysis indicates that mechanisms for the developmental increase in neuronal gap junction coupling directly control the death/survival mechanisms in developing neurons. Together, the results suggest a multitiered strategy for chemical synapses in developmental regulation of electrical synapses.}, }
@article {pmid21384257, year = {2011}, author = {Alexeeva, EI and Valieva, SI and Bzarova, TM and Semikina, EL and Isaeva, KB and Lisitsyn, AO and Denisova, RV and Chistyakova, EG}, title = {Efficacy and safety of repeat courses of rituximab treatment in patients with severe refractory juvenile idiopathic arthritis.}, journal = {Clinical rheumatology}, volume = {30}, number = {9}, pages = {1163-1172}, pmid = {21384257}, issn = {1434-9949}, mesh = {Adolescent ; Antibodies, Monoclonal, Murine-Derived/*therapeutic use ; Antirheumatic Agents/*therapeutic use ; Arthritis, Juvenile/*drug therapy/pathology/physiopathology ; B-Lymphocytes/drug effects ; Child ; Child, Preschool ; Drug Resistance, Multiple/drug effects ; Drug Therapy, Combination ; Female ; Humans ; Immunosuppressive Agents/*therapeutic use ; Joints/pathology/physiopathology ; Lymphocyte Depletion ; Male ; Remission Induction ; Rituximab ; Treatment Outcome ; }, abstract = {Treatment of severe juvenile idiopathic arthritis (JIA) represents a serious challenge. This study investigates the efficacy and safety of repeat courses of rituximab in patients with different forms of JIA refractory to infliximab and standard immunosuppressive therapy. Patients (n = 55; age 2.3-17.0 years) with severe polyarticular and systemic JIA (International League of Association for Rheumatology diagnostic criteria) received rituximab (one intravenous infusion/week for 4 weeks, 375 mg/m(2) per dose). Efficacy was assessed using the American College of Rheumatology Pediatric (ACR Pedi) criteria. The primary endpoint was an ACR Pedi 30 response at week 24. At week 24, ACR Pedi 30, 50, and 70 responses were achieved by 98%, 50%, and 40% of patients, respectively. By week 96, ACR Pedi 30, 50, and 70 responses were achieved by 98%, 93%, and 93% of 25 patients, respectively. Remission was recorded in 25%, 52%, 75%, and 98% of patients following the first (24 weeks), second (48 weeks), third (72 weeks), and fourth (96 weeks) courses of rituximab, respectively. Rituximab treatment significantly reduced the number of systemic manifestations at week 12 and also enabled 52% of patients to achieve remission of arthritis by week 48. This study supports the efficacy of rituximab in patients with severe forms of JIA, refractory to several prior agents.}, }
@article {pmid21332627, year = {2011}, author = {Nowicki, M and Rychlik, I and Haller, H and Warren, ML and Suchower, L and Gause-Nilsson, I and , }, title = {Saxagliptin improves glycaemic control and is well tolerated in patients with type 2 diabetes mellitus and renal impairment.}, journal = {Diabetes, obesity &amp; metabolism}, volume = {13}, number = {6}, pages = {523-532}, doi = {10.1111/j.1463-1326.2011.01382.x}, pmid = {21332627}, issn = {1463-1326}, mesh = {Adamantane/*analogs &amp; derivatives/therapeutic use ; Aged ; Blood Glucose/drug effects ; Diabetes Mellitus, Type 2/complications/*drug therapy ; Diabetic Nephropathies/blood/*drug therapy ; Dipeptides/*therapeutic use ; Dipeptidyl-Peptidase IV Inhibitors/*therapeutic use ; Double-Blind Method ; Female ; Glycated Hemoglobin A/*drug effects ; Humans ; Kidney Failure, Chronic/blood/*drug therapy ; Male ; Middle Aged ; Placebos ; Treatment Outcome ; }, abstract = {AIM: To evaluate the efficacy and safety of saxagliptin vs. placebo in patients with type 2 diabetes mellitus (T2DM) and renal impairment.<br><br>METHODS: In this multicentre, randomized, parallel-group, double-blind, placebo-controlled study, patients with glycated haemoglobin (HbA1c) 7-11% and creatinine clearance <50 ml/min were stratified by baseline renal impairment (moderate, severe or end-stage on haemodialysis), and randomized (1 : 1) to saxagliptin 2.5 mg once daily or placebo for 12 weeks. Oral antihyperglycaemic drugs and insulin therapy present at enrolment were continued throughout the study. The absolute change in HbA1c from baseline to week 12 (primary efficacy end-point) was analysed using an analysis of covariance model with last observation carried forward methodology.<br><br>RESULTS: A total of 170 patients were randomized and treated. The adjusted mean decrease from baseline to week 12 in HbA1c was statistically significantly greater in the saxagliptin group than in the placebo group; the difference between treatments was -0.42% (95% confidence interval: -0.71 to -0.12%, p = 0.007). Adjusted mean HbA1c decreases from baseline to week 12 were numerically greater with saxagliptin than with placebo in the subgroups of patients with moderate (-0.64 vs. -0.05%) and severe (-0.95 vs. -0.50%) renal impairment. HbA1c reductions were similar between saxagliptin and placebo in the subgroup with end-stage renal disease on haemodialysis (-0.84 vs. -0.87%). Saxagliptin was generally well tolerated; incidences of adverse events and hypoglycaemic events were similar to placebo.<br><br>CONCLUSIONS: Saxagliptin 2.5 mg once daily is a well-tolerated treatment option for patients with inadequately controlled T2DM and renal impairment.}, }
@article {pmid21203537, year = {2010}, author = {Derenko, M and Malyarchuk, B and Grzybowski, T and Denisova, G and Rogalla, U and Perkova, M and Dambueva, I and Zakharov, I}, title = {Origin and post-glacial dispersal of mitochondrial DNA haplogroups C and D in northern Asia.}, journal = {PloS one}, volume = {5}, number = {12}, pages = {e15214}, pmid = {21203537}, issn = {1932-6203}, mesh = {Asia ; Asian Continental Ancestry Group/genetics ; DNA, Mitochondrial/*genetics ; Emigration and Immigration ; Female ; Genetic Variation ; Genetics, Population ; Haplotypes ; Humans ; Phylogeny ; *Phylogeography ; Sequence Analysis, DNA ; }, abstract = {More than a half of the northern Asian pool of human mitochondrial DNA (mtDNA) is fragmented into a number of subclades of haplogroups C and D, two of the most frequent haplogroups throughout northern, eastern, central Asia and America. While there has been considerable recent progress in studying mitochondrial variation in eastern Asia and America at the complete genome resolution, little comparable data is available for regions such as southern Siberia--the area where most of northern Asian haplogroups, including C and D, likely diversified. This gap in our knowledge causes a serious barrier for progress in understanding the demographic pre-history of northern Eurasia in general. Here we describe the phylogeography of haplogroups C and D in the populations of northern and eastern Asia. We have analyzed 770 samples from haplogroups C and D (174 and 596, respectively) at high resolution, including 182 novel complete mtDNA sequences representing haplogroups C and D (83 and 99, respectively). The present-day variation of haplogroups C and D suggests that these mtDNA clades expanded before the Last Glacial Maximum (LGM), with their oldest lineages being present in the eastern Asia. Unlike in eastern Asia, most of the northern Asian variants of haplogroups C and D began the expansion after the LGM, thus pointing to post-glacial re-colonization of northern Asia. Our results show that both haplogroups were involved in migrations, from eastern Asia and southern Siberia to eastern and northeastern Europe, likely during the middle Holocene.}, }
@article {pmid21179161, year = {2010}, author = {Reich, D and Green, RE and Kircher, M and Krause, J and Patterson, N and Durand, EY and Viola, B and Briggs, AW and Stenzel, U and Johnson, PL and Maricic, T and Good, JM and Marques-Bonet, T and Alkan, C and Fu, Q and Mallick, S and Li, H and Meyer, M and Eichler, EE and Stoneking, M and Richards, M and Talamo, S and Shunkov, MV and Derevianko, AP and Hublin, JJ and Kelso, J and Slatkin, M and Pääbo, S}, title = {Genetic history of an archaic hominin group from Denisova Cave in Siberia.}, journal = {Nature}, volume = {468}, number = {7327}, pages = {1053-1060}, pmid = {21179161}, issn = {1476-4687}, support = {R01 GM040282/GM/NIGMS NIH HHS/United States ; R01-GM40282/GM/NIGMS NIH HHS/United States ; }, mesh = {Animals ; Asia ; DNA, Mitochondrial/genetics ; Europe ; Finger Phalanges/chemistry ; *Fossils ; *Gene Flow ; Genome/*genetics ; Hominidae/*classification/*genetics ; Humans ; Melanesia ; Molecular Sequence Data ; Phylogeny ; Siberia ; Tooth/anatomy &amp; histology/chemistry ; }, abstract = {Using DNA extracted from a finger bone found in Denisova Cave in southern Siberia, we have sequenced the genome of an archaic hominin to about 1.9-fold coverage. This individual is from a group that shares a common origin with Neanderthals. This population was not involved in the putative gene flow from Neanderthals into Eurasians; however, the data suggest that it contributed 4-6% of its genetic material to the genomes of present-day Melanesians. We designate this hominin population 'Denisovans' and suggest that it may have been widespread in Asia during the Late Pleistocene epoch. A tooth found in Denisova Cave carries a mitochondrial genome highly similar to that of the finger bone. This tooth shares no derived morphological features with Neanderthals or modern humans, further indicating that Denisovans have an evolutionary history distinct from Neanderthals and modern humans.}, }
@article {pmid21129766, year = {2011}, author = {Martinón-Torres, M and Dennell, R and Bermúdez de Castro, JM}, title = {The Denisova hominin need not be an out of Africa story.}, journal = {Journal of human evolution}, volume = {60}, number = {2}, pages = {251-255}, doi = {10.1016/j.jhevol.2010.10.005}, pmid = {21129766}, issn = {1095-8606}, mesh = {Africa ; *Animal Migration ; Animals ; DNA, Mitochondrial/genetics ; Fossils ; Hominidae/*genetics ; Siberia ; }, }
@article {pmid21105351, year = {2010}, author = {Bogomil'skiĭ, MR and Garashchenko, TI and Denisova, OA}, title = {[Assessment of the effectiveness of sinuforte used for the combined treatment of rhinosinusitis and exudative otitis media in children].}, journal = {Vestnik otorinolaringologii}, volume = {}, number = {4}, pages = {74-77}, pmid = {21105351}, issn = {0042-4668}, mesh = {Anti-Bacterial Agents/therapeutic use ; Child ; Child, Preschool ; Combined Modality Therapy ; *Cyclamen ; Female ; Humans ; Male ; Otitis Media with Effusion/drug therapy/surgery/*therapy ; Phytotherapy ; Plant Extracts/*therapeutic use ; Rhinitis/drug therapy/surgery/*therapy ; Sinusitis/drug therapy/surgery/*therapy ; Treatment Outcome ; }, abstract = {The objective of the present study was to evaluate efficiency, safety, and tolerability of two single doses of sinuforte (INVAR, Russia) used in the combined treatment of rhinosinusitis (including that with concurrent exudative otitis media) in children at the age from 5 to 12 years. The data obtained suggest high efficiency of sinuforte in the treatment of mild and moderately severe acute rhinosinusitis and exudative otitis media. Inclusion of sinuforte in combined therapy of these conditions makes it possible to avoid drainage procedures and decreases duration of antibacterial treatment. Simultaneous acceleration of the recovery of functional activity of endonasal mucosa suggests pronounced anti-recurrent action of sinuforte.}, }
@article {pmid21067548, year = {2010}, author = {Denisova, GF and Denisov, DA and Bramson, JL}, title = {Applying bioinformatics for antibody epitope prediction using affinity-selected mimotopes - relevance for vaccine design.}, journal = {Immunome research}, volume = {6 Suppl 2}, number = {}, pages = {S6}, pmid = {21067548}, issn = {1745-7580}, abstract = {To properly characterize protective polyclonal antibody responses, it is necessary to examine epitope specificity. Most antibody epitopes are conformational in nature and, thus, cannot be identified using synthetic linear peptides. Cyclic peptides can function as mimetics of conformational epitopes (termed mimotopes), thereby providing targets, which can be selected by immunoaffinity purification. However, the management of large collections of random cyclic peptides is cumbersome. Filamentous bacteriophage provides a useful scaffold for the expression of random peptides (termed phage display) facilitating both the production and manipulation of complex peptide libraries. Immunoaffinity selection of phage displaying random cyclic peptides is an effective strategy for isolating mimotopes with specificity for a given antiserum. Further epitope prediction based on mimotope sequence is not trivial since mimotopes generally display only small homologies with the target protein. Large numbers of unique mimotopes are required to provide sufficient sequence coverage to elucidate the target epitope. We have developed a method based on pattern recognition theory to deal with the complexity of large collections of conformational mimotopes. The analysis consists of two phases: 1) The learning phase where a large collection of epitope-specific mimotopes is analyzed to identify epitope specific "signs" and 2) The identification phase where immunoaffinity-selected mimotopes are interrogated for the presence of the epitope specific "signs" and assigned to specific epitopes. We are currently using computational methods to define epitope "signs" without the need for prior knowledge of specific mimotopes. This technology provides an important tool for characterizing the breadth of antibody specificities within polyclonal antisera.}, }
@article {pmid20943940, year = {2010}, author = {Wang, Y and Denisova, JV and Kang, KS and Fontes, JD and Zhu, BT and Belousov, AB}, title = {Neuronal gap junctions are required for NMDA receptor-mediated excitotoxicity: implications in ischemic stroke.}, journal = {Journal of neurophysiology}, volume = {104}, number = {6}, pages = {3551-3556}, pmid = {20943940}, issn = {1522-1598}, support = {HD-002528/HD/NICHD NIH HHS/United States ; P20 RR-024214/RR/NCRR NIH HHS/United States ; R01 DA-015088/DA/NIDA NIH HHS/United States ; R01 NS-064256/NS/NINDS NIH HHS/United States ; }, mesh = {Animals ; Apoptosis/drug effects ; Blood-Brain Barrier ; Brain Ischemia/chemically induced/*physiopathology ; Cells, Cultured/drug effects/physiology ; Connexins/deficiency ; Dentate Gyrus/drug effects/pathology ; Dizocilpine Maleate/pharmacology/therapeutic use ; Excitatory Amino Acid Antagonists/pharmacology/therapeutic use ; Gap Junctions/drug effects/*physiology ; Male ; Mefloquine/pharmacology/therapeutic use ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; N-Methylaspartate/pharmacokinetics/*toxicity ; Nerve Degeneration/*chemically induced/prevention &amp; control ; Neurons/*physiology ; Neurotoxins/pharmacokinetics/*toxicity ; Patch-Clamp Techniques ; Photochemistry ; Receptors, N-Methyl-D-Aspartate/drug effects/*physiology ; Rose Bengal/radiation effects/toxicity ; }, abstract = {N-methyl-D-aspartate receptors (NMDARs) play an important role in cell survival versus cell death decisions during neuronal development, ischemia, trauma, and epilepsy. Coupling of neurons by electrical synapses (gap junctions) is high or increases in neuronal networks during all these conditions. In the developing CNS, neuronal gap junctions are critical for two different types of NMDAR-dependent cell death. However, whether neuronal gap junctions play a role in NMDAR-dependent neuronal death in the mature CNS was not known. Using Fluoro-Jade B staining, we show that a single intraperitoneal administration of NMDA (100 mg/kg) to adult wild-type mice induces neurodegeneration in three forebrain regions, including rostral dentate gyrus. However, the NMDAR-mediated neuronal death is prevented by pharmacological blockade of neuronal gap junctions (with mefloquine, 30 mg/kg) and does not occur in mice lacking neuronal gap junction protein, connexin 36. Using Western blots, electrophysiology, calcium imaging, and gas chromatography-mass spectrometry in wild-type and connexin 36 knockout mice, we show that the reduced level of neuronal death in knockout animals is not caused by the reduced expression of NMDARs, activity of NMDARs, or permeability of the blood-brain barrier to NMDA. In wild-type animals, this neuronal death is not caused by upregulation of connexin 36 by NMDA. Finally, pharmacological and genetic inactivation of neuronal gap junctions in mice also dramatically reduces neuronal death caused by photothrombotic focal cerebral ischemia. The results indicate that neuronal gap junctions are required for NMDAR-dependent excitotoxicity and play a critical role in ischemic neuronal death.}, }
@article {pmid20726964, year = {2010}, author = {Malyarchuk, B and Derenko, M and Denisova, G and Wozniak, M and Grzybowski, T and Dambueva, I and Zakharov, I}, title = {Phylogeography of the Y-chromosome haplogroup C in northern Eurasia.}, journal = {Annals of human genetics}, volume = {74}, number = {6}, pages = {539-546}, doi = {10.1111/j.1469-1809.2010.00601.x}, pmid = {20726964}, issn = {1469-1809}, mesh = {Chromosomes, Human, Y/*genetics ; Ethnic Groups/genetics ; Europe, Eastern ; Far East ; Genetic Variation ; *Haplotypes ; Humans ; Male ; Microsatellite Repeats ; *Phylogeography ; Polymorphism, Single Nucleotide ; Siberia ; }, abstract = {To reconstruct the phylogenetic structure of Y-chromosome haplogroup (hg) C in populations of northern Eurasia, we have analyzed the diversity of microsatellite (STR) loci in a total sample of 413 males from 18 ethnic groups of Siberia, Eastern Asia and Eastern Europe. Analysis of SNP markers revealed that all Y-chromosomes studied belong to hg C3 and its subhaplogroups C3c and C3d, although some populations (such as Mongols and Koryaks) demonstrate a relatively high input (more than 30%) of yet unidentified C3* haplotypes. Median joining network analysis of STR haplotypes demonstrates that Y-chromosome gene pools of populations studied are characterized by the presence of DNA clusters originating from a limited number of frequent founder haplotypes. These are subhaplogroup C3d characteristic for Mongolic-speaking populations, "star cluster" in C3* paragroup, and a set of DYS19 duplicated C3c Y-chromosomes. All these DNA clusters show relatively recent coalescent times (less than 3000 years), so it is probable that founder effects, including social selection resulting in high male fertility associated with a limited number of paternal lineages, may explain the observed distribution of hg C3 lineages.}, }
@article {pmid20697660, year = {2010}, author = {Tarakina, NV and Neder, RB and Denisova, TA and Maksimova, LG and Baklanova, YV and Tyutyunnik, AP and Zubkov, VG}, title = {Defect crystal structure of new TiO(OH)2 hydroxide and related lithium salt Li2TiO3.}, journal = {Dalton transactions (Cambridge, England : 2003)}, volume = {39}, number = {35}, pages = {8168-8176}, doi = {10.1039/c0dt00354a}, pmid = {20697660}, issn = {1477-9234}, abstract = {Crystal structures of TiO(OH)(2) and Li(2)TiO(3) have been studied in detail and refined using X-ray powder diffraction data. Both compounds possess a high concentration of defects in the structure. The crystal structure of the Li(2)TiO(3) salt obtained at 700 degrees C reveals stacking faults of LiTi(2) metal layers, which leads to the appearance of short-range order in three possible space groups: C2/c, C2/m, P3(1)12. The possibility to stabilise this imperfect state increases the mobility of the Li(+) ions in the structure and allows the complete exchange of lithium by hydrogen in acid water solutions with formation of TiO(OH)(2). The crystal structure of TiO(OH)(2) belongs to the layered double hydroxide structure type with the 3R(1) sequence of oxygen layers and can be described as a stacking of charge-neutral metal oxyhydroxide slabs [(OH)(2)OTi(2)O(OH)(2)]. TiO(OH)(2) is the first layered double hydroxide structure formed by a cation with oxidation state +4 only.}, }
@article {pmid20488990, year = {2010}, author = {Gibson, DG and Glass, JI and Lartigue, C and Noskov, VN and Chuang, RY and Algire, MA and Benders, GA and Montague, MG and Ma, L and Moodie, MM and Merryman, C and Vashee, S and Krishnakumar, R and Assad-Garcia, N and Andrews-Pfannkoch, C and Denisova, EA and Young, L and Qi, ZQ and Segall-Shapiro, TH and Calvey, CH and Parmar, PP and Hutchison, CA and Smith, HO and Venter, JC}, title = {Creation of a bacterial cell controlled by a chemically synthesized genome.}, journal = {Science (New York, N.Y.)}, volume = {329}, number = {5987}, pages = {52-56}, doi = {10.1126/science.1190719}, pmid = {20488990}, issn = {1095-9203}, mesh = {Bacterial Proteins/analysis ; Base Sequence ; *Bioengineering ; Cloning, Molecular ; DNA, Bacterial/chemical synthesis/genetics ; Escherichia coli/genetics ; Gene Deletion ; Genes, Bacterial ; *Genetic Engineering ; *Genome, Bacterial ; Molecular Sequence Data ; Mycoplasma capricolum/*genetics ; Mycoplasma mycoides/*genetics/growth &amp; development/physiology/ultrastructure ; Phenotype ; Plasmids ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Saccharomyces cerevisiae/genetics ; Transformation, Bacterial ; }, abstract = {We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including "watermark" sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.}, }
@article {pmid20457583, year = {2010}, author = {Malyarchuk, B and Derenko, M and Denisova, G and Kravtsova, O}, title = {Mitogenomic diversity in Tatars from the Volga-Ural region of Russia.}, journal = {Molecular biology and evolution}, volume = {27}, number = {10}, pages = {2220-2226}, doi = {10.1093/molbev/msq065}, pmid = {20457583}, issn = {1537-1719}, mesh = {Base Sequence ; DNA, Mitochondrial/*genetics ; Ethnic Groups/*genetics ; Evolution, Molecular ; *Genetic Variation ; Genetics, Population ; Haplotypes/genetics ; Humans ; Models, Genetic ; Molecular Sequence Data ; *Phylogeny ; Polymorphism, Restriction Fragment Length ; Russia ; Sequence Analysis, DNA ; }, abstract = {To investigate diversity of mitochondrial gene pool of Tatars inhabiting the territory of the middle Volga River basin, 197 individuals from two populations representing Kazan Tatars and Mishars were subjected for analysis of mitochondrial DNA (mtDNA) control region variation. In addition, 73 mitochondrial genomes of individuals from Mishar population were sequenced completely. It was found that mitochondrial gene pool of the Volga Tatars consists of two parts, but western Eurasian component prevails considerably (84% on average) over eastern Asian one (16%). Eastern Asian mtDNAs detected in Tatars belonged to a heterogeneous set of haplogroups (A, C, D, G, M7, M10, N9a, Y, and Z), although only haplogroups A and D were revealed simultaneously in both populations. Complete mtDNA variation study revealed that the age of western Eurasian haplogroups (such as U4, HV0a, and H) is less than 18,000 years, thus suggesting re-expansion of eastern Europeans soon after the Last Glacial Maximum.}, }
@article {pmid20336068, year = {2010}, author = {Krause, J and Fu, Q and Good, JM and Viola, B and Shunkov, MV and Derevianko, AP and Pääbo, S}, title = {The complete mitochondrial DNA genome of an unknown hominin from southern Siberia.}, journal = {Nature}, volume = {464}, number = {7290}, pages = {894-897}, pmid = {20336068}, issn = {1476-4687}, mesh = {Africa/ethnology ; Animals ; DNA, Mitochondrial/*genetics/isolation &amp; purification ; Emigration and Immigration ; Extinction, Biological ; Finger Phalanges ; Genome, Mitochondrial/*genetics ; Hominidae/*classification/*genetics ; Humans ; Molecular Sequence Data ; *Phylogeny ; Sequence Alignment ; Siberia ; Time Factors ; }, abstract = {With the exception of Neanderthals, from which DNA sequences of numerous individuals have now been determined, the number and genetic relationships of other hominin lineages are largely unknown. Here we report a complete mitochondrial (mt) DNA sequence retrieved from a bone excavated in 2008 in Denisova Cave in the Altai Mountains in southern Siberia. It represents a hitherto unknown type of hominin mtDNA that shares a common ancestor with anatomically modern human and Neanderthal mtDNAs about 1.0 million years ago. This indicates that it derives from a hominin migration out of Africa distinct from that of the ancestors of Neanderthals and of modern humans. The stratigraphy of the cave where the bone was found suggests that the Denisova hominin lived close in time and space with Neanderthals as well as with modern humans.}, }
@article {pmid20211840, year = {2010}, author = {Benders, GA and Noskov, VN and Denisova, EA and Lartigue, C and Gibson, DG and Assad-Garcia, N and Chuang, RY and Carrera, W and Moodie, M and Algire, MA and Phan, Q and Alperovich, N and Vashee, S and Merryman, C and Venter, JC and Smith, HO and Glass, JI and Hutchison, CA}, title = {Cloning whole bacterial genomes in yeast.}, journal = {Nucleic acids research}, volume = {38}, number = {8}, pages = {2558-2569}, pmid = {20211840}, issn = {1362-4962}, mesh = {Base Sequence ; Cloning, Molecular/*methods ; Diploidy ; Genetic Vectors/chemistry ; *Genome, Bacterial ; Molecular Sequence Data ; Mycoplasma/*genetics ; Mycoplasma genitalium/genetics ; Mycoplasma mycoides/genetics ; Mycoplasma pneumoniae/genetics ; Recombination, Genetic ; Saccharomyces cerevisiae/*genetics ; }, abstract = {Most microbes have not been cultured, and many of those that are cultivatable are difficult, dangerous or expensive to propagate or are genetically intractable. Routine cloning of large genome fractions or whole genomes from these organisms would significantly enhance their discovery and genetic and functional characterization. Here we report the cloning of whole bacterial genomes in the yeast Saccharomyces cerevisiae as single-DNA molecules. We cloned the genomes of Mycoplasma genitalium (0.6 Mb), M. pneumoniae (0.8 Mb) and M. mycoides subspecies capri (1.1 Mb) as yeast circular centromeric plasmids. These genomes appear to be stably maintained in a host that has efficient, well-established methods for DNA manipulation.}, }
@article {pmid21626818, year = {2010}, author = {Doronin, BM and Denisova, OA}, title = {[Disturbances of lipid metabolism in the period of age androgen deficit in middle-aged and elderly men with ischemic stroke].}, journal = {Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova}, volume = {110}, number = {12 Pt 2}, pages = {52-54}, pmid = {21626818}, issn = {1997-7298}, mesh = {Aged ; Androgens/blood/deficiency ; Cholesterol/blood ; Humans ; Hyperlipidemias/*blood ; Intracranial Arteriosclerosis/*blood/*epidemiology ; Lipoproteins, LDL/blood ; Male ; Middle Aged ; Stroke/*blood ; Testosterone/blood/*deficiency ; Triglycerides/blood ; }, abstract = {Blood concentrations of total cholesterol, beta-lipoproteins, triglycerides and testosterone were studied in 73 men (mean age 65.9 +/- 8.6 years) with acute ischemic stroke. The concentrations of testosterone and triglycerides in men decrease with age and those of cholesterol and beta-lipoproteins do not depend on the age. In middle-aged patients, the negative correlations were found between concentrations of testosterone and beta-lipoproteins (r = -0.404; p < 0.05); testosterone and cholesterol (r = -0.273; p < 0.001). In elderly patients, there were negative correlations between concentrations of testosterone and triglycerides (r = -0.348; p < 0.05). In middle-aged and elderly patients, concentrations of triglycerides and testosterone were negatively correlated (r = -0.478; p < 0.001). The lower blood testosterone level was associated with the higher risk of the development of atherosclerotic lesions of major arteries that allowed to estimate risks of ischemic stroke in middle-aged and elderly men.}, }
@article {pmid19882902, year = {2009}, author = {Baranova, EN and Sharipova, IN and Denisova, NM and Susekina, ME and Puzyrev, VF and Sarkisian, KA and Vorob'eva, MS and Burkov, AN and Ulanova, TI}, title = {[The capacities of current test systems to verify early HIV infection].}, journal = {Voprosy virusologii}, volume = {54}, number = {5}, pages = {37-40}, pmid = {19882902}, issn = {0507-4088}, mesh = {AIDS Serodiagnosis/*methods ; HIV Antibodies/blood ; HIV Core Protein p24/blood ; HIV Infections/blood/*diagnosis ; HIV-1/*immunology ; HIV-2/*immunology ; Humans ; Immunoblotting ; Immunoenzyme Techniques/*methods ; *Reagent Kits, Diagnostic ; Sensitivity and Specificity ; }, abstract = {The purpose of the present investigation was to comparatively evaluate the performance characteristics of the test systems designed to verify the positive results of screening survey for HIV infection, such as the solid-phase immunoassay DS-EIA-HIV-AB/AG-SPECTR (Diagnosticheskiye Sistemy (Diagnostic Systems) Research-and-Production Association, Nizhni Novgorod) and tests based on immune blotting (IB). The investigation examined 15 seroconversion panels produced by ZeptoMetrix (USA) and BBI (USA). The use of the DS-EIA-HIV-AB/AG-SPECTR test system determined 88 of the 167 seroconversion panels as HIV positive. The IB-based tests revealed only 45 of the 167 samples as positive. Consequently, the application of the DS-EIA-HIV-AB/AG-SPECTR test system is more effective than the IB-based tests in early HIV infection.}, }
@article {pmid19795792, year = {2009}, author = {Zemlianskikh, NG and Denisova, ON}, title = {[Changes in erythrocyte membrane-cytoskeleton complex, induced by dimethyl sulfoxide, polyethylene glycol, and low temperature].}, journal = {Biofizika}, volume = {54}, number = {4}, pages = {693-703}, pmid = {19795792}, issn = {0006-3029}, mesh = {Animals ; Cryoprotective Agents/*chemistry/pharmacology ; Cytoskeleton/*chemistry/metabolism ; Dimethyl Sulfoxide/*chemistry/pharmacology ; Dogs ; Erythrocyte Membrane/*chemistry/metabolism ; Freezing ; Oxidation-Reduction/drug effects ; Polyethylene Glycols/*chemistry/pharmacology ; Surface-Active Agents/*chemistry/pharmacology ; }, abstract = {The effect of the cryoprotectants DMSO and PEG-1500 as well as freezing-thawing on the proteins of the canine erythrocyte membrane-cytoskeleton complex has been studied using the cross-linking reagent diamide. It was shown that the intensity of distortions in the protein network structure correlated to an increase in SH-group accessibility for the oxidation by this compound and accordingly, the enhancement of the formation of high-molecular-weight protein aggregates. The maximum level of diamide-induced aggregability was revealed upon freezing of erythrocytes in liquid nitrogen without the cryoprotectant. The electrophoretical analysis of the ghost proteins of erythrocytes incubated with cryoprotectants showed a significant increase in the aggregation level only in cells present in the polymer solution. After the freezing-thawing cycle, the diamide-induced protein aggregability in erythrocytes cryopreserved with PEG-1500 strongly increased, while if DMSO was used for cell protection, the level of aggregates was much less pronounced than in the control. One can suppose that the exocellular cryoprotectant PEG-1500, as distinct from the endocellular cryoprotectant DMSO, is unable to provide the preservation of the structure of the membrane-cytoskeleton protein complex at the acceptable level necessary for the maintenance of cell integrity after the return to the physiological conditions.}, }
@article {pmid19705797, year = {2009}, author = {Stotskaia, TV and Mravian, SR and Vishniakova, MV and Vashchenko, AV and Stepanova, EA and Denisova, LB and Grigor'eva, NM}, title = {[Kawasaki disease in an adult European].}, journal = {Klinicheskaia meditsina}, volume = {87}, number = {7}, pages = {63-66}, pmid = {19705797}, issn = {0023-2149}, mesh = {Adult ; European Continental Ancestry Group ; Humans ; Male ; Mucocutaneous Lymph Node Syndrome/*diagnosis/drug therapy ; }, abstract = {A case of Kawasaki disease is reported with manifestations of autoimmune vasculitis and predominant affection of coronary arteries. The relevant literature review describes major symptoms of this pathology and risk factors of its atypical forms. It is emphasized that rare occurrence of Kawasaki disease among non-mongoloid subjects should not make the general practitioner les suspicious of this condition.}, }
@article {pmid19696314, year = {2009}, author = {Lartigue, C and Vashee, S and Algire, MA and Chuang, RY and Benders, GA and Ma, L and Noskov, VN and Denisova, EA and Gibson, DG and Assad-Garcia, N and Alperovich, N and Thomas, DW and Merryman, C and Hutchison, CA and Smith, HO and Venter, JC and Glass, JI}, title = {Creating bacterial strains from genomes that have been cloned and engineered in yeast.}, journal = {Science (New York, N.Y.)}, volume = {325}, number = {5948}, pages = {1693-1696}, doi = {10.1126/science.1173759}, pmid = {19696314}, issn = {1095-9203}, mesh = {Centromere ; *Cloning, Molecular ; DNA Methylation ; DNA Restriction Enzymes/genetics/metabolism ; Deoxyribonucleases, Type III Site-Specific/genetics ; *Gene Transfer Techniques ; *Genetic Engineering ; *Genome, Bacterial ; Mycoplasma capricolum/*genetics ; Mycoplasma mycoides/*genetics/growth &amp; development/isolation &amp; purification ; Plasmids ; Saccharomyces cerevisiae/*genetics ; Sequence Analysis, DNA ; Sequence Deletion ; Transformation, Bacterial ; }, abstract = {We recently reported the chemical synthesis, assembly, and cloning of a bacterial genome in yeast. To produce a synthetic cell, the genome must be transferred from yeast to a receptive cytoplasm. Here we describe methods to accomplish this. We cloned a Mycoplasma mycoides genome as a yeast centromeric plasmid and then transplanted it into Mycoplasma capricolum to produce a viable M. mycoides cell. While in yeast, the genome was altered by using yeast genetic systems and then transplanted to produce a new strain of M. mycoides. These methods allow the construction of strains that could not be produced with genetic tools available for this bacterium.}, }
@article {pmid19566074, year = {2009}, author = {Denisova, EA and Kirichenko, LL and Rushkevich, OP}, title = {[Structural and functional changes in heart under medical therapy in mental workers having arterial hypertension].}, journal = {Meditsina truda i promyshlennaia ekologiia}, volume = {}, number = {5}, pages = {23-26}, pmid = {19566074}, issn = {1026-9428}, mesh = {Antihypertensive Agents/*therapeutic use ; Disease Progression ; Echocardiography ; Electrocardiography ; Female ; Follow-Up Studies ; Heart Ventricles/*diagnostic imaging/physiopathology ; Humans ; Hypertension/*drug therapy/etiology/physiopathology ; Male ; Middle Aged ; Occupational Diseases/*drug therapy/etiology/physiopathology ; Prognosis ; Stress, Psychological/*complications/physiopathology ; Ventricular Function, Left/*physiology ; }, abstract = {Aim of the study was to study main structural and functional parameters of heart, evaluate efficiency of treatment (Egiloc, Cardilopin, their combination) in arterial hypertension patients. Examinations including ECG, EchoCG were performed initially, in 3 and 6 months after the treatment start. The study covered 126 patients with arterial hypertension II degree, who received the medium-dose therapy, and 34 apparently healthy individuals.}, }
@article {pmid19537091, year = {2009}, author = {Lideman, LF and Denisov, DA and Gibadulin, RA and Denisova, GF}, title = {[Autoimmune response in HIV-infected patients is directed against CD4 domain 4].}, journal = {Voprosy virusologii}, volume = {54}, number = {3}, pages = {12-16}, pmid = {19537091}, issn = {0507-4088}, mesh = {Alanine/genetics ; Algorithms ; Amino Acid Sequence ; Amino Acid Substitution ; *Autoimmunity ; CD4 Antigens/chemistry/genetics/*immunology ; Cell Line ; Epitope Mapping ; Epitopes, T-Lymphocyte/chemistry/genetics/*immunology ; HIV Infections/*immunology ; HIV-1/*immunology ; Humans ; Models, Molecular ; Peptide Library ; Protein Structure, Tertiary/genetics ; }, abstract = {Phage display epitope library technology and a novel computer algorithm have been used for the localization of CD4 epitopes specific for monoclonal antibody (mAb) T6 and autoimmune antibodies found in an HIV infected patient. Both predicted epitope clusters have been shown to overlap and to be localized within the domain 4 of CD4. They included Cys303, Glu304, Glu330, and Lys331 amino acids. Few amino acids predicted by the algorithm as the epitope residues and two residues that did not relate to the epitope were sequentially substituted for Ala. Further analysis of the mutated forms of sCD4 expressed in 293T cells transfected with the corresponding DNAs, supported the predicted localization of the mAb T6 epitope. The results demonstrate that the autoimmune response in HIV-infected patients is directed against domain D4 of sCD4.}, }
@article {pmid19526099, year = {2008}, author = {Cheknyov, SB and Babayants, AA and Denisova, EA}, title = {Induction of interferon production by conformation-modified proteins of plasma gamma-globulin fraction.}, journal = {Bulletin of experimental biology and medicine}, volume = {146}, number = {5}, pages = {591-595}, doi = {10.1007/s10517-009-0349-x}, pmid = {19526099}, issn = {0007-4888}, mesh = {Cells, Cultured ; Copper/metabolism ; Gene Expression Regulation/*drug effects ; Humans ; Interferon-gamma/metabolism ; Interferons/*metabolism ; Leukocytes/*drug effects/*metabolism ; Protein Binding ; Protein Conformation ; Zinc/metabolism ; gamma-Globulins/*chemistry/metabolism/*pharmacology ; }, abstract = {Plasma gamma-globulin fraction proteins, copper and zinc cations, and metal-modified gamma-globulins induce the production of IFN by human leukocytes. Binding of zinc cations attenuates the realization of the IFN-inducing effects of human serum gamma-globulin, while binding of copper cations potentiated this effect. Activity of IFN and the dynamics of its production correspond to those in response to phytohemagglutinin stimulation. The pool of induced IFN contains acid-labile (up to 60%) and acid-stable (up to 40%) constituents. Anti-IFN-alpha antibodies do not modify activity of produced IFN. The results indicate the possibility of gamma-globulin conformation allowing stimulation or attenuation of the protein capacity to induce the production of IFN pool with predominant content of IFN-gamma.}, }
@article {pmid19518411, year = {2009}, author = {Denisov, SI and Lyutyy, TV and Denisova, ES and Hänggi, P and Kantz, H}, title = {Directed transport in periodically rocked random sawtooth potentials.}, journal = {Physical review. E, Statistical, nonlinear, and soft matter physics}, volume = {79}, number = {5 Pt 1}, pages = {051102}, doi = {10.1103/PhysRevE.79.051102}, pmid = {19518411}, issn = {1539-3755}, abstract = {We study directed transport of overdamped particles in a periodically rocked random sawtooth potential. Two transport regimes can be identified which are characterized by a nonzero value of the average velocity of particles and a zero value, respectively. The properties of directed transport in these regimes are investigated both analytically and numerically in terms of a random sawtooth potential and a periodically varying driving force. Precise conditions for the occurrence of transition between these two transport regimes are derived and analyzed in detail.}, }
@article {pmid19514440, year = {2009}, author = {Denisova, EN and Sharipova, VR and Purlo, NV and Sukhanova, GA and Biriukova, LS}, title = {[Use of fractional plasma separation and adsorption (Prometheus technology) in the treatment of acute liver failure].}, journal = {Anesteziologiia i reanimatologiia}, volume = {}, number = {2}, pages = {45-49}, pmid = {19514440}, issn = {0201-7563}, mesh = {Adolescent ; Adsorption ; Adult ; Aged ; Bilirubin/blood ; Extracorporeal Circulation/instrumentation/*methods ; Female ; Humans ; Liver Failure, Acute/blood/etiology/*therapy ; Male ; Middle Aged ; *Plasma ; Sorption Detoxification/instrumentation/*methods ; Treatment Outcome ; Young Adult ; }, abstract = {This paper presents the results of treating 8 patients with acute liver failure, by using the separation and adsorption of fractional plasma (Prometheus technology). Twenty-five procedures lasting 5-6 hours were performed. Anticoagulation with heparin was made under guidance of coagulogram parameters. The results of testing blood parameters before and after a procedure and hemodynamic parameters are given. The investigations have demonstrated the effectiveness and safety of the procedure.}, }
@article {pmid19390580, year = {2009}, author = {Denisova, G and Denisov, D and Evelegh, C and Weissgram, M and Beck, J and Foley, SR and Bramson, JL}, title = {Characterizing complex polysera produced by antigen-specific immunization through the use of affinity-selected mimotopes.}, journal = {PloS one}, volume = {4}, number = {4}, pages = {e5309}, pmid = {19390580}, issn = {1932-6203}, mesh = {Algorithms ; Animals ; Cell Proliferation ; Epitopes/*chemistry/*immunology ; Immune Sera/*immunology ; Immunization ; Mice ; Mice, Inbred BALB C ; Mice, Transgenic ; Peptide Library ; Rats ; Receptor, ErbB-2/metabolism ; }, abstract = {BACKGROUND: Antigen-based (as opposed to whole organism) vaccines are actively being pursued for numerous indications. Even though different formulations may produce similar levels of total antigen-specific antibody, the composition of the antibody response can be quite distinct resulting in different levels of therapeutic activity.<br><br>Using plasmid-based immunization against the proto-oncogene HER-2 as a model, we have demonstrated that affinity-selected epitope mimetics (mimotopes) can provide a defined signature of a polyclonal antibody response. Further, using novel computer algorithms that we have developed, these mimotopes can be used to predict epitope targets.<br><br>CONCLUSIONS/SIGNIFICANCE: By combining our novel strategy with existing methods of epitope prediction based on physical properties of an individual protein, we believe that this method offers a robust method for characterizing the breadth of epitope-specificity within a specific polyserum. This strategy is useful as a tool for monitoring immunity following vaccination and can also be used to define relevant epitopes for the creation of novel vaccines.}, }
@article {pmid19297016, year = {2009}, author = {Denisova, LA and Maev, RG and Leontjev, VK and Denisov, AF and Grayson, GG and Rusanov, FS and Bakulin, EY and Gavrilov, DY and Grineva, TV}, title = {A study of the adhesion between dental cement and dentin using a nondestructive acoustic microscopy approach.}, journal = {Dental materials : official publication of the Academy of Dental Materials}, volume = {25}, number = {5}, pages = {557-565}, doi = {10.1016/j.dental.2008.10.011}, pmid = {19297016}, issn = {1879-0097}, mesh = {Adhesiveness ; *Dental Bonding ; Dental Stress Analysis ; Dentin/*diagnostic imaging ; *Dentin-Bonding Agents ; Glass Ionomer Cements ; Humans ; Materials Testing/methods ; *Microscopy, Acoustic ; Tensile Strength ; }, abstract = {OBJECTIVES: The goal of the present study was to investigate the potential for acoustic microscopy techniques to characterize the cement-dentin interface in restored teeth.<br><br>METHODS: Special flat-parallel specimens and whole extracted teeth with restorations were scanned using a high-frequency (50 MHz) focused ultrasonic transducer. Visual acoustic images (B- and C-scans) of the cement-dentin interface were obtained nondestructively, analyzed and compared with optical images taken after the samples were cut along the scanning axis. The shear bonding strength of a subsection of specimens was tested in a Lloyd material testing machine.<br><br>RESULTS: An essential distinction between the acoustical properties associated with good and failed bonding has been shown. In the case of failed adhesion, the ultrasound signal reflection from the cement-dentin interface is up to four to seven times higher in magnitude than in the case of good bonding. The comparison of the ultrasound imaging data with the data obtained using an optical microscope revealed a strong correspondence with the acoustical and optical results with respect to the presence, position and dimensions of the defects. The specimens showing higher ultrasound reflection from cement/dentin interface have also shown lower shear bonding strength.<br><br>SIGNIFICANCE: The results demonstrate that acoustic scanning with a high-frequency focused ultrasonic probe is a valuable method for nondestructive morpho-mechanical analysis of cement/dentin interface for either experimental models or whole restored teeth. An appropriately expanded approach can be widely used for the pre-clinical evaluation of dental materials. Further, this method may prove beneficial in the design of new diagnostic ultrasound devices and techniques for use within clinical dentistry.}, }
@article {pmid19145899, year = {2008}, author = {Denisova, TP and Tiul'tiaeva, LA}, title = {[Peptic ulcer in industrial region: 25-year survey].}, journal = {Eksperimental'naia i klinicheskaia gastroenterologiia = Experimental &amp; clinical gastroenterology}, volume = {}, number = {4}, pages = {21-24}, pmid = {19145899}, issn = {1682-8658}, mesh = {Data Interpretation, Statistical ; Health Surveys ; Humans ; *Industry ; Kinetics ; Peptic Ulcer/*epidemiology/etiology/mortality ; Russia/epidemiology ; Severity of Illness Index ; Socioeconomic Factors ; }, abstract = {In the article the dynamics of gravity of the course of ulcer among the inhabitants of industrial region during the 25-summer period is analyzed, including the years of social stability, social stress and output from it. The index of the lethality of the patients with ulcer is used as the criterion of gravity. Temporary dependences and speed characteristics of studied statistical data underwent analysis. The interrelation of gravity of the course of ulcer with the social life of society is established.}, }
@article {pmid20201400, year = {2009}, author = {Rachina, SA and Fokin, AA and Mishchenko, VV and Ishmukhametov, AA and Denisova, MN}, title = {[Comparative analysis of the use of antimicrobials with systemic action in outpatients in the Russian Federation, Ukraine and Kazakhstan].}, journal = {Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic]}, volume = {54}, number = {7-8}, pages = {24-29}, pmid = {20201400}, issn = {0235-2990}, mesh = {*Ambulatory Care ; Anti-Bacterial Agents/*administration &amp; dosage ; Drug Utilization/*statistics &amp; numerical data ; Humans ; Kazakhstan ; Russia ; Ukraine ; }, }
@article {pmid20201309, year = {2009}, author = {Baryshnikova, NV and Denisova, EV and Kornienko, EA and Lobach, SM and Loeva, IA and Parolova, NI and Ryzhikh, VA and Solonitsin, EG and Smirnova, AS and Starostin, BD and Suvorov, AN and Suvorova, MA and Tkachenko, EI and Uspenskiĭ, IuP}, title = {[Epidemiological research of Helicobacter pylori resistance to clarithromycin at inhabitants of Sankt of Petersburg with a stomach ulcer].}, journal = {Eksperimental'naia i klinicheskaia gastroenterologiia = Experimental &amp; clinical gastroenterology}, volume = {}, number = {5}, pages = {73-76}, pmid = {20201309}, issn = {1682-8658}, mesh = {Anti-Bacterial Agents/*administration &amp; dosage ; Clarithromycin/*administration &amp; dosage ; *Drug Resistance, Bacterial ; Female ; *Helicobacter Infections/drug therapy/epidemiology ; *Helicobacter pylori ; Humans ; Male ; Russia/epidemiology ; *Stomach Ulcer/drug therapy/epidemiology/microbiology ; Urban Population ; }, }
@article {pmid19110593, year = {2008}, author = {Cheknyov, SB and Denisova, EA and Mongush, EM and Shukhovtseva, YV}, title = {The Results of Mancini's test depend on the presence bound metal cations in the test protein.}, journal = {Bulletin of experimental biology and medicine}, volume = {145}, number = {4}, pages = {457-459}, doi = {10.1007/s10517-008-0117-3}, pmid = {19110593}, issn = {0007-4888}, mesh = {Blood Chemical Analysis/*methods/standards ; Cations/chemistry/*metabolism/pharmacology ; Copper/chemistry/metabolism ; Humans ; Metals/chemistry/*metabolism/pharmacology ; Osmolar Concentration ; Protein Binding ; Serologic Tests/methods/standards ; Zinc/chemistry/metabolism ; gamma-Globulins/*analysis/*metabolism ; }, abstract = {Samples of human serum gamma-globulin with specifically bound copper or zinc cations were studied in Mancini's radial immunodiffusion test with human antibodies to IgG (H+L). The intensity of antibody binding to zinc-modified protein was 10-20% higher in comparison with the reference sample, while detection of gamma-globulin with bound copper by antibodies was 20-30% lower than in the corresponding reference sample. Comparison with the results of native gamma-globulin testing indicates limitations of Mancini's method as the quantitative assay for practical diagnosis, because under certain clinical conditions the traditional method can give over- and underestimated results.}, }
@article {pmid19038455, year = {2009}, author = {Denisov, DA and Denisova, GF and Lelic, A and Loeb, MB and Bramson, JL}, title = {Deciphering epitope specificities within polyserum using affinity selection of random peptides and a novel algorithm based on pattern recognition theory.}, journal = {Molecular immunology}, volume = {46}, number = {3}, pages = {429-436}, doi = {10.1016/j.molimm.2008.10.013}, pmid = {19038455}, issn = {0161-5890}, support = {N01-AI-40066/AI/NIAID NIH HHS/United States ; }, mesh = {*Algorithms ; Amino Acid Sequence ; Chromatography, Affinity ; Computational Biology ; Epitopes/chemistry/*immunology ; Humans ; Immune Sera/*immunology ; Molecular Sequence Data ; *Pattern Recognition, Automated ; Peptides/chemistry/*immunology/*isolation &amp; purification ; West Nile Fever/immunology ; West Nile virus/chemistry/immunology ; }, abstract = {While numerous strategies have been developed to map epitope specificities for monoclonal antibodies, few have been designed for elucidating epitope specificity within complex polysera. We have developed a novel algorithm based on pattern recognition theory that can be used to characterize the breadth of epitope specificities within a polyserum based on affinity selection of random peptides. To attribute these random peptides to a specific epitope, the sequences of the affinity-selected peptides were matched against a database of random peptides selected using well-described monoclonal antibodies. To test this novel algorithm, we employed polyserum from patients infected with West Nile virus and isolated 109 unique sequences which were recognized selectively by serum from West Nile virus-infected patients but not uninfected patients. Through application of our algorithm, it was possible to match 20% of the polyserum-selected peptides to the database of peptides isolated by affinity selection using monoclonal antibodies against the virus envelope protein. Statistical analysis demonstrated that the peptides selected with the polyserum could not be attributed to the peptide database by chance. This novel algorithm provides the basis for further development of methods to characterize the breadth of epitope recognition within a complex pool of antibodies.}, }
@article {pmid18942387, year = {2008}, author = {Sidorenko, SV and Agapova, ED and Aleksandrova, IA and Bichul', OK and Vereshchagina, SA and Galeeva, EV and Denisova, OI and Eremina, LV and Ivanov, DV and Ivanova, TA and Ivanova, SF and Katosova, LK and Kirilin, DN and Kostina, GV and Kozlova, TA and Makeeva, NN and Marusina, NE and Monakhova, SI and Morozova, NA and Moskvitina, EN and Plakhoshchniuk, L and Rozanova, SM and Solov'eva, TD and Sukhoreva, MV and Sychev, IN and Tikhonov, IuG and Shumilova, MO and Furletova, NM and Shustrova, TA}, title = {[Cross and associated antibiotic resistance of gramnegative bacteria of the family Enterobacteriaceae resistant to the 3rd generation cephalosporins].}, journal = {Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic]}, volume = {53}, number = {1-2}, pages = {10-18}, pmid = {18942387}, issn = {0235-2990}, mesh = {Anti-Bacterial Agents/*pharmacology ; Cephalosporins/*pharmacology ; Drug Resistance, Bacterial/*physiology ; Enterobacteriaceae/*enzymology/growth &amp; development/*isolation &amp; purification ; }, }
@article {pmid18825996, year = {2008}, author = {Denisova, TV and Kazeev, KSh and Kolesnikov, SI and Val'kov, VF}, title = {[The stability of enzyme activity and the content of microflora of different soils of the South of Russia to influence of a variable magnetic field of industrial frequency].}, journal = {Radiatsionnaia biologiia, radioecologiia}, volume = {48}, number = {4}, pages = {481-486}, pmid = {18825996}, issn = {0869-8031}, mesh = {Bacteria/isolation &amp; purification/metabolism/*radiation effects ; *Electromagnetic Fields ; Enzyme Stability ; Enzymes/metabolism/*radiation effects ; Fungi/isolation &amp; purification/metabolism/*radiation effects ; Russia ; Soil/analysis ; *Soil Microbiology ; }, abstract = {In modelling experiments of the influence of variable magnetic field of industrial frequency (50 Hz) by induction of 1500 and 6000 mkTl during 5 days on microflora and on enzyme activity of soils the South of Russia of different genesis and properties is investigated.}, }
@article {pmid18825995, year = {2008}, author = {Rogacheva, SM and Denisova, SA and Shul'gin, SV and Somov, AIu and Kuznetsov, PE}, title = {[Biological effects of electromagnetic radiation of extremely high frequencies combined with physiologically active compounds].}, journal = {Radiatsionnaia biologiia, radioecologiia}, volume = {48}, number = {4}, pages = {474-480}, pmid = {18825995}, issn = {0869-8031}, mesh = {Animals ; Antiprotozoal Agents/*pharmacology ; Cell Movement/drug effects/radiation effects ; Dose-Response Relationship, Drug ; Dose-Response Relationship, Radiation ; Hydrogen Bonding/drug effects/radiation effects ; Metronidazole/*pharmacology ; *Microwaves ; Nicotine/*pharmacology ; Paramecium caudatum/*drug effects/*radiation effects ; Time Factors ; }, abstract = {The study of the action of the electromagnetic radiation (EMR) of low intensity (10 microW/cm2) in the range of frequencies 120-170 GHz at the test-reaction of Infusoria Paramecium caudatum was carried out. The resonant character of the effects was established. The EMR action at 156.6 and 161.3 GHz caused the increase of infusorians mobility, the action at frequencies 151.8, 155.7, 167.1 GHz caused the mobility reduction. Isolated and combined with EMR effects of nicotine (10(-4)-10(-15) mol/l) and antimicrobial drug metronidazole (10(-5), 10(-8), 10(-9) mol/l) were investigated. The radiation at the frequency 167.1 GHz was shown to reduce the effect of nicotine (10(-9) mol/l) and to enhance the effect of metronidazole (10(-9) mol/l). This phenomenon may be explained by different effects of the substances in low concentration at the water hydrogen bonds net structure.}, }
@article {pmid18778753, year = {2008}, author = {Arumugam, H and Denisova, JV and Neve, RL and Corriveau, RA and Belousov, AB}, title = {Use of calcium imaging for analysis of neuronal gap junction coupling.}, journal = {Neuroscience letters}, volume = {445}, number = {1}, pages = {26-30}, pmid = {18778753}, issn = {0304-3940}, support = {P20 RR016816/RR/NCRR NIH HHS/United States ; R01 DA015088/DA/NIDA NIH HHS/United States ; R01 DA015088-05/DA/NIDA NIH HHS/United States ; P20RR16816/RR/NCRR NIH HHS/United States ; }, mesh = {6-Cyano-7-nitroquinoxaline-2,3-dione/pharmacology ; Analysis of Variance ; Anesthetics/pharmacology ; Animals ; Bicuculline/pharmacology ; CREB-Binding Protein/metabolism/pharmacology ; Calcium/*metabolism ; Cells, Cultured ; Connexins/deficiency ; Diagnostic Imaging/*methods ; Embryo, Mammalian ; Excitatory Amino Acid Antagonists/pharmacology ; GABA Antagonists/pharmacology ; Gap Junctions/*metabolism ; Glycyrrhetinic Acid/pharmacology ; Halothane/pharmacology ; Hypothalamus, Middle/cytology ; Mice ; Mice, Knockout ; Neurons/drug effects/*metabolism ; Rats ; Rats, Sprague-Dawley ; Receptors, N-Methyl-D-Aspartate/deficiency ; Valine/analogs &amp; derivatives/pharmacology ; }, abstract = {We recently used Western blots for connexin 36 and neuronal dye coupling with neurobiotin to measure developmental decrease in neuronal gap junction coupling in cell cultures. To ask whether Ca2+ imaging also can be used to measure changes in the amount of neuronal gap junction coupling, we defined a Ca2+ coupling coefficient as the percentage of neurons with bicuculline-induced increases in intracellular Ca2+ that are suppressed by blocking gap junctions. We demonstrate in rat and mouse hypothalamic neuronal cultures that the Ca2+ coupling coefficient decreases during culture development, this decrease is prevented by manipulations that also prevent developmental decrease in neuronal gap junction coupling, and the coefficient is low in cultures lacking connexin 36. The results indicate that Ca2+ imaging is a useful tool to quantify the amount of neuronal gap junction coupling in cultures.}, }
@article {pmid18760481, year = {2008}, author = {Denisova, GF and Denisov, DA and Yeung, J and Loeb, MB and Diamond, MS and Bramson, JL}, title = {A novel computer algorithm improves antibody epitope prediction using affinity-selected mimotopes: a case study using monoclonal antibodies against the West Nile virus E protein.}, journal = {Molecular immunology}, volume = {46}, number = {1}, pages = {125-134}, pmid = {18760481}, issn = {0161-5890}, support = {N01AI40066/AI/NIAID NIH HHS/United States ; U01 AI061373/AI/NIAID NIH HHS/United States ; U01 AI061373-04/AI/NIAID NIH HHS/United States ; AI-061373/AI/NIAID NIH HHS/United States ; }, mesh = {*Algorithms ; Amino Acid Sequence ; Antibodies, Monoclonal/*immunology ; Antibody Affinity/*immunology ; *Computers ; Epitopes/chemistry/*immunology ; Models, Molecular ; Molecular Sequence Data ; Structural Homology, Protein ; Viral Proteins/chemistry/*immunology ; West Nile virus/*immunology ; }, abstract = {Understanding antibody function is often enhanced by knowledge of the specific binding epitope. Here, we describe a computer algorithm that permits epitope prediction based on a collection of random peptide epitopes (mimotopes) isolated by antibody affinity purification. We applied this methodology to the prediction of epitopes for five monoclonal antibodies against the West Nile virus (WNV) E protein, two of which exhibit therapeutic activity in vivo. This strategy was validated by comparison of our results with existing F(ab)-E protein crystal structures and mutational analysis by yeast surface display. We demonstrate that by combining the results of the mimotope method with our data from mutational analysis, epitopes could be predicted with greater certainty. The two methods displayed great complementarity as the mutational analysis facilitated epitope prediction when the results with the mimotope method were equivocal and the mimotope method revealed a broader number of residues within the epitope than the mutational analysis. Our results demonstrate that the combination of these two prediction strategies provides a robust platform for epitope characterization.}, }
@article {pmid18686374, year = {2007}, author = {Avakian, GN and Denisova, SV and Oleĭnikova, OM and Kurbanova, SA}, title = {[A role of circadian factors and melatonin in the development of epileptic paroxysms].}, journal = {Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova}, volume = {107}, number = {9}, pages = {85-92}, pmid = {18686374}, issn = {1997-7298}, mesh = {Brain/*metabolism ; Circadian Rhythm/*physiology ; Disease Progression ; Epilepsy/*metabolism/*physiopathology ; Humans ; Melatonin/*metabolism ; Prognosis ; }, }
@article {pmid18494287, year = {2008}, author = {Ivanov, SV and Zhabin, SN and Gorbacheva, OS and Barsukov, VS and Denisova, EIu}, title = {[Multifactor analysis of nodular goiter by the method of artificial neural network].}, journal = {Klinicheskaia meditsina}, volume = {86}, number = {4}, pages = {49-50}, pmid = {18494287}, issn = {0023-2149}, mesh = {Biopsy, Needle ; Diagnosis, Differential ; Follow-Up Studies ; Goiter, Nodular/*diagnosis/surgery ; Humans ; *Neural Networks, Computer ; Predictive Value of Tests ; Prognosis ; Reproducibility of Results ; Retrospective Studies ; }, abstract = {Study of 297 patients with preparative methods of nontoxic nodular goiter and cytological diagnose "adenomatosis" with the use of artificial neural network (ANN) were analyzed. Malignant pathology could be supposed with 95.5% accuracy when we applied experience of trained ANN. Clinical, instrumental and cytologic criteria, which make possible to prognosticate degree of malignant process in thyroid gland parenchyma, were determined. Painless elastic nodules with sonographically iso- o hyperechogenic structure, with clear outline and cytologically determined adenomatosis in combination with colloid can be interpreted as benign lesion of thyroid gland.}, }
@article {pmid18454113, year = {2008}, author = {Denisova, LA and Maev, RG and Leont'ev, VK and Denisov, AF and Greĭson, GG and Rusanov, FS and Bakulin, EIu and Gavrilov, DIu and Grineva, TV}, title = {[Acoustic microscopy application for the evaluation of restorative materials bonding quality. Experimental investigation].}, journal = {Stomatologiia}, volume = {87}, number = {2}, pages = {15-24}, pmid = {18454113}, issn = {0039-1735}, mesh = {Dental Caries/*diagnostic imaging/therapy ; Dental Materials/*analysis ; Humans ; Microscopy, Acoustic/*methods ; *Models, Biological ; }, abstract = {Ultrasound transmission and reflection at the interface between restorative material and tooth tissue have been investigated using experimental flat-parallel models and whole extracted human teeth with restorations. It has been found that in the case of good bonding the ultrasound wave energy practically does not reflect at the cement/dentin interface and propagates into dentin layer. If any microscopic cavity had been formed between cement and dentin, the most part of the acoustic energy would be reflected from the interface between cement and gaseous or liquid contents of that cavity. The results acquired have been used for the interpretation of morphological peculiarities of the adhesive bonding area in visual images, non-destructively obtained in acoustic microscope ultrasound frequency - 50 MHz. The differences discovered could be used as a base for the design and development of new ultrasound methods for the restorative materials bonding evaluation as well as for secondary caries revealing.}, }
@article {pmid18339829, year = {2008}, author = {Webb, E and Kuh, D and Peasey, A and Pajak, A and Malyutina, S and Kubinova, R and Topor-Madry, R and Denisova, D and Capkova, N and Marmot, M and Bobak, M}, title = {Childhood socioeconomic circumstances and adult height and leg length in central and eastern Europe.}, journal = {Journal of epidemiology and community health}, volume = {62}, number = {4}, pages = {351-357}, doi = {10.1136/jech.2006.056457}, pmid = {18339829}, issn = {1470-2738}, support = {R01 AG023522-01/AG/NIA NIH HHS/United States ; G19/35//Medical Research Council/United Kingdom ; G0100222//Medical Research Council/United Kingdom ; G8802774//Medical Research Council/United Kingdom ; R01 AG023522/AG/NIA NIH HHS/United States ; R01 AG013196/AG/NIA NIH HHS/United States ; RG/07/008/23674//British Heart Foundation/United Kingdom ; }, mesh = {Aged ; Anthropometry ; Body Height ; Child ; Educational Status ; Europe, Eastern/epidemiology ; Female ; Household Articles/statistics &amp; numerical data ; Humans ; Leg/*growth &amp; development ; Male ; Middle Aged ; Parents ; *Social Class ; Urban Health ; }, abstract = {BACKGROUND: Adult height and leg length have been shown to be positively associated with childhood socioeconomic circumstances in several studies in western populations. This study will determine whether similar associations are observable in settings with different social histories, and will assess whether adult leg length is more strongly associated than adult height.<br><br>METHODS: Random samples of men and women aged 45-69 years were taken from population registers in Novosibirsk (Russia), Krakow (Poland) and six towns of the Czech Republic, recruiting nearly 29,000 people. Participants completed a questionnaire that included questions regarding their mother's and father's education (not available in the Czech Republic) and ownership of several household items when they were 10 years old. Participants' standing and sitting heights were measured and from these an estimate of leg length was derived. Associations between indicators of childhood socioeconomic circumstances and anthropometric measures were analysed using linear regression.<br><br>RESULTS: Russian individuals were shorter and reported fewer household assets at the age of 10 years than Czech and Polish individuals. Parental education and household assets were strongly associated with each other and both were independently associated with height, leg length and trunk length. Height was associated with childhood circumstances more strongly than leg length. The associations of childhood circumstances with the leg/trunk ratio were weak and inconsistent.<br><br>CONCLUSION: In these urban populations in eastern Europe, adult height is associated with childhood conditions at least as strongly as leg length.}, }
@article {pmid18322006, year = {2008}, author = {Liu, X and Popescu, IR and Denisova, JV and Neve, RL and Corriveau, RA and Belousov, AB}, title = {Regulation of cholinergic phenotype in developing neurons.}, journal = {Journal of neurophysiology}, volume = {99}, number = {5}, pages = {2443-2455}, pmid = {18322006}, issn = {0022-3077}, support = {R01 DA015088/DA/NIDA NIH HHS/United States ; P20 RR 16816/RR/NCRR NIH HHS/United States ; R01 DA 015088/DA/NIDA NIH HHS/United States ; }, mesh = {Acetylcholine/physiology ; Animals ; Calcium/metabolism ; Cells, Cultured ; Cyclic AMP Response Element-Binding Protein/physiology ; Cyclic AMP-Dependent Protein Kinases/physiology ; Dizocilpine Maleate/pharmacology ; Electrophysiology ; Excitatory Amino Acid Antagonists/pharmacology ; Excitatory Postsynaptic Potentials/physiology ; Extracellular Signal-Regulated MAP Kinases/physiology ; Glutamic Acid/physiology ; Immunohistochemistry ; In Vitro Techniques ; Male ; Mice ; Mice, Knockout ; Mitogen-Activated Protein Kinases/physiology ; NF-kappa B/physiology ; Neurons/drug effects/*physiology ; Parasympathetic Nervous System/*cytology/drug effects/*physiology ; Phenotype ; Rats ; Rats, Sprague-Dawley ; Receptors, Cholinergic/physiology ; Receptors, N-Methyl-D-Aspartate/genetics/physiology ; Synaptic Transmission/physiology ; Transfection ; }, abstract = {Specification of neurotransmitter phenotype is critical for neural circuit development and is influenced by intrinsic and extrinsic factors. Recent findings in rat hypothalamus in vitro suggest the role of neurotransmitter glutamate in the regulation of cholinergic phenotype. Here we extended our previous studies on the mechanisms of glutamate-dependent regulation of cholinergic phenotypic properties in hypothalamic neurons. Using immunocytochemistry, electrophysiology, and calcium imaging, we demonstrate that hypothalamic expression of choline acetyltransferase (the cholinergic marker) and responsiveness of neurons to acetylcholine (ACh) receptor agonists increase during chronic administration of an N-methyl-D-aspartate receptor (NMDAR) blocker, MK-801, in developing rats in vivo and genetic and pharmacological inactivation of NMDARs in mouse and rat developing neuronal cultures. In hypothalamic cultures, an inactivation of NMDA receptors also induces ACh-dependent synaptic activity, as do inactivations of PKA, ERK/MAPK, CREB, and NF-kappaB, which are known to be regulated by NMDA receptors. Interestingly, the increase in cholinergic properties in developing neurons that is induced by NMDAR blockade is prevented by the blockade of ACh receptors, suggesting that function of ACh receptor is required for the cholinergic up-regulation. Using dual recording of monosynaptic excitatory postsynaptic currents, we further demonstrate that chronic inactivation of ionotropic glutamate receptors induces the cholinergic phenotype in a subset of glutamatergic neurons. The phenotypic switch is partial as ACh and glutamate are coreleased. The results suggest that developing neurons may not only coexpress multiple transmitter phenotypes, but can also change the phenotypes following changes in signaling in neuronal circuits.}, }
@article {pmid18318223, year = {2008}, author = {Filippov, AV and Ovsiankina, ES and Denisova, LS and Stakheeva, LB}, title = {[Comparative analysis of tuberculosis morbidity in children and adolescents from Moscow and the Penza Region].}, journal = {Problemy tuberkuleza i boleznei legkikh}, volume = {}, number = {1}, pages = {6-10}, pmid = {18318223}, issn = {1728-2993}, mesh = {Adolescent ; Catchment Area, Health ; Child ; Female ; Humans ; Incidence ; Male ; Mass Screening ; Prevalence ; Russia/epidemiology ; Tuberculosis, Pulmonary/*epidemiology ; }, abstract = {Tuberculosis morbidity was comparatively analyzed in the children and adolescents of Moscow and the Penza Region. The epidemiology of tuberculosis in the children and adolescents was established to be universal in the compared regions in the study period. This manifested itself as a stable tendency for higher morbidity rates (MR), increased incidence of destructive tuberculosis, inadequate use of the existing methods for active detection of tuberculosis in these age groups, and, among those who had fallen ill with this disease, an increase in the number of persons from the foci of tuberculous infection and persons with established contact with patients with tuberculosis. Along with a steady MR growth for tuberculosis among the children and adolescents, there has been a quantitative modification to the proportion of all epidemiologically hazardous forms of tuberculosis, such as the bacillary and destructive forms of pulmonary tuberculosis complicated by extrapulmonary tuberculosis. Hypodiagnosis of tuberculous pleurisy and extrapulmonary tuberculosis has been recently noted in the general health care network. The analysis also leads to the conclusion that the quality of mass tuberculin diagnosis remains poor. In both regions, general pediatricians are not adequately alert to tuberculosis, extrapulmonary tuberculosis in particular. With a rise in tuberculosis endemic, the incidence of tuberculosis in 18-21-year-old persons is 2-3 times greater than that in adolescents, which is associated with the greater activity of this population group and with the underdetection of the asymptomatic forms of tuberculosis in adolescents.}, }
@article {pmid18279453, year = {2008}, author = {Kulagin, A and Lisukov, I and Denisova, V and Kruchkova, I and Sizikova, S and Gilevich, A and Pronkina, N and Kozhevnikov, V and Kozlov, V}, title = {Severe pancytopenia associated with a minor paroxysmal nocturnal haemoglobinuria clone and response to ciclosporin A in a heavily pretreated multiple myeloma patient.}, journal = {British journal of haematology}, volume = {141}, number = {1}, pages = {122-123}, doi = {10.1111/j.1365-2141.2008.07003.x}, pmid = {18279453}, issn = {1365-2141}, mesh = {Cyclosporine/*therapeutic use ; Hemoglobinuria, Paroxysmal/*drug therapy ; Humans ; Immunosuppressive Agents/*therapeutic use ; Male ; Middle Aged ; Multiple Myeloma/*drug therapy ; Pancytopenia/*drug therapy ; }, }
@article {pmid18260893, year = {2007}, author = {Karpov, IuA and Deev, AD and , }, title = {[PRIVILEGIYA (Privilege) study - Prestarium in the treatment of arterial hypertension: antihypertensive effectiveness and safety in comparison with enalapril].}, journal = {Kardiologiia}, volume = {47}, number = {7}, pages = {35-40}, pmid = {18260893}, issn = {0022-9040}, mesh = {Adult ; Aged ; Angiotensin-Converting Enzyme Inhibitors/therapeutic use ; Antihypertensive Agents/administration &amp; dosage/adverse effects/*therapeutic use ; Drug Administration Schedule ; Enalapril/administration &amp; dosage/adverse effects/*therapeutic use ; Female ; Humans ; Hypertension/*drug therapy ; Male ; Middle Aged ; Perindopril/administration &amp; dosage/adverse effects/*therapeutic use ; Russia ; Treatment Outcome ; }, }
@article {pmid18260880, year = {2007}, author = {Denisova, DV and Nikitin, IuP and Zav'ialova, LG and Burakova, SV and Ivanova, MV}, title = {[Secular trends in blood lipid disorders among adolescents: population study in Novosibirsk (1989-2003)].}, journal = {Kardiologiia}, volume = {47}, number = {6}, pages = {62-67}, pmid = {18260880}, issn = {0022-9040}, mesh = {Adolescent ; Dyslipidemias/blood/*epidemiology ; Female ; Humans ; Lipids/*blood ; Male ; Morbidity/trends ; Population Surveillance/*methods ; Retrospective Studies ; Siberia/epidemiology ; }, abstract = {UNLABELLED: Hyperlipidemia is a known risk factor of coronary artery disease. The reduction of elevated serum total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) levels in childhood may reduce cardiovascular morbidity and mortality in adulthood. Aim of the study was to assess prevalence and trends in lipid profile and lipid disorders in adolescent population in Novosibirsk (1989-2003).<br><br>METHODS: Four cross-sectional surveys of school children aged 14-17 years in 1989 (n=656), in 1994 (n=620), in 1999 (n=626) and in 2003 (n=667) were carried out. Total sample was 2569 (1214 males and 1355 females). Blood total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), triglycerides (TG) were measured by enzymatic methods. LDL-C was calculated with Friedwald's formula. Associations of blood lipids with age, sex, year of study, body mass index (kg/m2), blood pressure and main nutrients of diet were measured by GLM method. Prevalence of dyslipidemia was evaluated with NCEP-peds criteria. Diet was estimated using 24-hour dietary recall.<br><br>RESULTS: During the 15-year period (1989-2003) mean serum TC decreased from 175 to 162 mg/dl in males and from 191 to 175 mg/dl in females (p < 0.001). Average levels of LDL-C also fell significantly during this period, but no changes in HDL-C levels were found. Females had significantly higher mean TC and LDL-C than did males at all surveys (p < 0.001). According to NCFP-peds criteria prevalence of high TC (200 mg/dl und more) during the period (1989-2003) significantly decreased from 22% to 8% (p < 0.01) in males and from 32% to 17% (p < 0.05) in females. Frequencies of low HDL-C (< 40 mg/dl) also decreased in boys and girls (p < 0.05 for both gender groups). Significant regression coefficients for TC controlled by age, sex and year of study were revealed with body mass index, diastolic blood pressure and triceps skinfolds. Trends in diet during the period showed significant decrease of total energy and basic nutrient intakes (proteins, fats and carbohydrates).<br><br>CONCLUSION: During the period of socioeconomic reforms in Russia (1989-2003) the lipid profile and prevalence of atherogenic dislipidemias in adolescents of Novosibirsk significantly changed following the changes of body mass index and diet.}, }
@article {pmid18218864, year = {2008}, author = {Gibson, DG and Benders, GA and Andrews-Pfannkoch, C and Denisova, EA and Baden-Tillson, H and Zaveri, J and Stockwell, TB and Brownley, A and Thomas, DW and Algire, MA and Merryman, C and Young, L and Noskov, VN and Glass, JI and Venter, JC and Hutchison, CA and Smith, HO}, title = {Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.}, journal = {Science (New York, N.Y.)}, volume = {319}, number = {5867}, pages = {1215-1220}, doi = {10.1126/science.1151721}, pmid = {18218864}, issn = {1095-9203}, mesh = {Base Sequence ; Chromosomes, Artificial, Bacterial ; Chromosomes, Artificial, Yeast ; *Cloning, Molecular ; DNA, Bacterial/*chemical synthesis ; DNA, Recombinant ; Escherichia coli/genetics ; Genetic Vectors ; *Genome, Bacterial ; Genomics/*methods ; Mycoplasma genitalium/*genetics ; Oligodeoxyribonucleotides/chemical synthesis ; Plasmids ; Recombination, Genetic ; Saccharomyces cerevisiae/genetics ; Sequence Analysis, DNA ; Transformation, Genetic ; }, abstract = {We have synthesized a 582,970-base pair Mycoplasma genitalium genome. This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type M. genitalium G37 except MG408, which was disrupted by an antibiotic marker to block pathogenicity and to allow for selection. To identify the genome as synthetic, we inserted "watermarks" at intergenic sites known to tolerate transposon insertions. Overlapping "cassettes" of 5 to 7 kilobases (kb), assembled from chemically synthesized oligonucleotides, were joined by in vitro recombination to produce intermediate assemblies of approximately 24 kb, 72 kb ("1/8 genome"), and 144 kb ("1/4 genome"), which were all cloned as bacterial artificial chromosomes in Escherichia coli. Most of these intermediate clones were sequenced, and clones of all four 1/4 genomes with the correct sequence were identified. The complete synthetic genome was assembled by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae, then isolated and sequenced. A clone with the correct sequence was identified. The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments.}, }
@article {pmid19555046, year = {2008}, author = {Denisova, EA and Kirichenko, LL and Struchkov, PV}, title = {[Structural functional state of cardiovascular system in hypertensive patients].}, journal = {Terapevticheskii arkhiv}, volume = {80}, number = {9}, pages = {84-86}, pmid = {19555046}, issn = {0040-3660}, mesh = {Humans ; *Hypertension/complications/pathology/physiopathology ; *Hypertrophy, Left Ventricular/etiology/pathology/physiopathology ; Myocardium/*pathology ; Prognosis ; *Vascular Diseases/etiology/pathology/physiopathology ; Vasodilation/physiology ; }, }
@article {pmid18154131, year = {2007}, author = {Malinova, LI and Sergeeva, IuV and Simonenko, GV and Denisova, TP and Dovgalevskiĭ, PIa and Tuchin, VV}, title = {[Plasma dehydratation self-organization in patients with coronary heart].}, journal = {Klinicheskaia laboratornaia diagnostika}, volume = {}, number = {10}, pages = {14-16}, pmid = {18154131}, issn = {0869-2084}, mesh = {Adult ; Aged ; Aged, 80 and over ; Coronary Disease/*diagnosis ; Female ; Humans ; Male ; Middle Aged ; *Plasma ; }, abstract = {The functional morphology of biological fluids is a basically new branch of science, which is based on the study of a result of phase change processes in the substrate under study. The paper shows the specific features of plasma spatial organization in patients with chronic and acute coronary heart disease (CHD). The findings may be used as an additional criterion for the differential diagnosis of the above forms of CHD and open up a new line in the diagnosis and risk stratification in CHD patients.}, }
@article {pmid18019017, year = {2007}, author = {Silkov, AN and Gavrilenko, VA and Denisova, VV and Grishina, LV and Kozlov, VA and Sennikov, SV}, title = {Effects of recombinant IL-4delta2 on human peripheral blood mononuclears.}, journal = {Bulletin of experimental biology and medicine}, volume = {143}, number = {1}, pages = {72-74}, doi = {10.1007/s10517-007-0020-3}, pmid = {18019017}, issn = {0007-4888}, mesh = {Alternative Splicing ; Cell Proliferation/drug effects ; Humans ; Immunoglobulin E/biosynthesis/blood ; In Vitro Techniques ; Interleukin-4/genetics/*pharmacology ; Interleukin-6/biosynthesis/blood/genetics ; Leukocytes, Mononuclear/cytology/*drug effects/metabolism ; Recombinant Proteins/pharmacology ; }, abstract = {In human cells, expression of IL-4 gene involves alternative mRNA splicing. IL-4delta2 splice variant is an antagonist of full-length IL-4 protein and blocks its effect on the functional activity of immunocompetent cells. The effect of recombinant IL-4delta2 on cytokine-producing activity of human peripheral blood mononuclear cells is shown for the first time.}, }
@article {pmid17970206, year = {2007}, author = {Denisova, VV and Kulagin, AD and Lisukov, IA and Kryuchkova, IV and Sizikova, SA and Sennikov, SV and Kozlov, VA}, title = {Cytokine-producing activity of bone marrow erythrokaryocytes and its regulation under normal conditions.}, journal = {Bulletin of experimental biology and medicine}, volume = {143}, number = {2}, pages = {218-221}, doi = {10.1007/s10517-007-0055-5}, pmid = {17970206}, issn = {0007-4888}, mesh = {Bone Marrow Cells/*metabolism ; Chemotactic Factors/metabolism ; Cytokines/*metabolism ; Erythrocytes/*metabolism ; Glycophorins/metabolism ; Granulocyte Colony-Stimulating Factor/metabolism ; Granulocyte-Macrophage Colony-Stimulating Factor/metabolism ; Humans ; Interferon-gamma/metabolism ; Interleukin-10/metabolism ; Interleukin-13/metabolism ; Interleukin-17/metabolism ; Interleukin-1beta/metabolism ; Interleukin-4/metabolism ; Interleukin-6/metabolism ; Interleukin-8/metabolism ; Macrophage Inflammatory Proteins/metabolism ; Tumor Necrosis Factor-alpha/metabolism ; }, abstract = {We showed that inhibition of cytokine production by bone marrow adherent cells and their soluble products is a mechanism of regulation of cytokine-producing activity of bone marrow erythrokaryocytes under normal conditions.}, }
@article {pmid17970204, year = {2007}, author = {Cheknyov, SB and Babayeva, EE and Denisova, EA and Vorobyova, UA and Mongush, EM}, title = {Antigenic specificity of human serum gamma-globulin samples obtained under conditions of equimolar binding of copper and zinc cations.}, journal = {Bulletin of experimental biology and medicine}, volume = {143}, number = {2}, pages = {210-213}, doi = {10.1007/s10517-007-0053-7}, pmid = {17970204}, issn = {0007-4888}, mesh = {Copper/*metabolism ; Enzyme-Linked Immunosorbent Assay ; Epitopes/chemistry/immunology/metabolism ; Humans ; Protein Binding ; Protein Conformation ; Spectrophotometry, Ultraviolet ; Zinc/*metabolism ; gamma-Globulins/chemistry/immunology/*metabolism ; }, abstract = {Samples of human serum gamma-globulin modified by equimolar binding of copper and zinc cations were obtained using the method of molecular ultrafiltration. Conformation characteristics of the protein were studied by UV spectrophotometry. Immunochemical study included radial immunodiffusion test, direct and sandwich enzyme immunoassays. Conformation changes in gamma-globulin caused by incorporation of solitary metal cations into the protein molecule modified presentation of antigenic determinants on the globule surface and their availability for recognition by specific antibodies. New antigenic determinants and new antigenic specificity of gamma-globulin are not formed under these conditions.}, }
@article {pmid17930193, year = {2007}, author = {Denisov, SI and Kostur, M and Denisova, ES and Hänggi, P}, title = {Arrival time distribution for a driven system containing quenched dichotomous disorder.}, journal = {Physical review. E, Statistical, nonlinear, and soft matter physics}, volume = {76}, number = {3 Pt 1}, pages = {031101}, doi = {10.1103/PhysRevE.76.031101}, pmid = {17930193}, issn = {1539-3755}, abstract = {We study the arrival time distribution of overdamped particles driven by a constant force in a piecewise linear random potential which generates the dichotomous random force. Our approach is based on the path integral representation of the probability density of the arrival time. We explicitly calculate the path integral for a special case of dichotomous disorder and use the corresponding characteristic function to derive prominent properties of the arrival time probability density. Specifically, we establish the scaling properties of the central moments, analyze the behavior of the probability density for short, long, and intermediate distances. In order to quantify the deviation of the arrival time distribution from a Gaussian shape, we evaluate the skewness and the kurtosis.}, }
@article {pmid17929618, year = {2007}, author = {Denisova, DV and Nikitin, YP and Zavjalova, LG and Burakova, SV}, title = {Trends in cardiovascular risk factors of Siberian adolescents during socioeconomic reforms in Russia (1989-2003).}, journal = {Alaska medicine}, volume = {49}, number = {2 Suppl}, pages = {110-116}, pmid = {17929618}, issn = {0002-4538}, mesh = {Adolescent ; Age Factors ; Body Mass Index ; Cardiovascular Diseases/*epidemiology ; Cross-Sectional Studies ; Female ; Health Surveys ; Humans ; Male ; Motor Activity ; Nutritional Status ; *Politics ; Prevalence ; Risk Factors ; Russia/epidemiology ; Siberia/epidemiology ; Social Class ; }, abstract = {OBJECTIVE: Prevalence and trends of cardiovascular risk factors among adolescent population in Novosibirsk during the reforms in Russia (1989-2003).<br><br>STUDY DESIGN: Four cross-sectional surveys of representative samples of schoolchildren aged 14-17 since 1989 (total--2,569).<br><br>METHODS: Body mass index (BMI) was calculated (kg/m2), blood pressure was double measured with Korotkov's method, and serum total cholesterol (TC) and high-density lipoprotein cholesterol (HDL-C) were estimated with enzymatic methods. Low-density lipoprotein cholesterol (LDL-C) was calculated with Friedwald's formula. Self-report of smoking (1 cig/week and more) and physical inactivity (2 h/week and less) was obtained. Diet was estimated using 24-hour dietary recall.<br><br>RESULTS: Prevalence of high TC, high LDL-C and low HDL-C significantly decreased in both gender groups. Prevalence of arterial hypertension also decreased in boys and girls. Frequency of overweight decreased more in girls. Trends in diet showed significant decreasing of total energy and basic nutrient intakes. Cigarette smoking rate decreased in boys and increased in girls. Physical inactivity rate was high in boys and girls.<br><br>CONCLUSION: Obtained data indicate similar trends to reduction in classical CVD risk factors, energy intake and life style changes among Siberian adolescents during the period of socioeconomic reforms in Russia.}, }
@article {pmid17924343, year = {2007}, author = {Derenko, M and Malyarchuk, B and Grzybowski, T and Denisova, G and Dambueva, I and Perkova, M and Dorzhu, C and Luzina, F and Lee, HK and Vanecek, T and Villems, R and Zakharov, I}, title = {Phylogeographic analysis of mitochondrial DNA in northern Asian populations.}, journal = {American journal of human genetics}, volume = {81}, number = {5}, pages = {1025-1041}, pmid = {17924343}, issn = {0002-9297}, mesh = {Asia ; Asian Continental Ancestry Group/*genetics ; DNA, Mitochondrial/*genetics ; European Continental Ancestry Group/genetics ; Genetic Variation ; *Geography ; Haplotypes ; Humans ; Molecular Sequence Data ; *Phylogeny ; Principal Component Analysis ; Sequence Analysis, DNA ; }, abstract = {To elucidate the human colonization process of northern Asia and human dispersals to the Americas, a diverse subset of 71 mitochondrial DNA (mtDNA) lineages was chosen for complete genome sequencing from the collection of 1,432 control-region sequences sampled from 18 autochthonous populations of northern, central, eastern, and southwestern Asia. On the basis of complete mtDNA sequencing, we have revised the classification of haplogroups A, D2, G1, M7, and I; identified six new subhaplogroups (I4, N1e, G1c, M7d, M7e, and J1b2a); and fully characterized haplogroups N1a and G1b, which were previously described only by the first hypervariable segment (HVS1) sequencing and coding-region restriction-fragment-length polymorphism analysis. Our findings indicate that the southern Siberian mtDNA pool harbors several lineages associated with the Late Upper Paleolithic and/or early Neolithic dispersals from both eastern Asia and southwestern Asia/southern Caucasus. Moreover, the phylogeography of the D2 lineages suggests that southern Siberia is likely to be a geographical source for the last postglacial maximum spread of this subhaplogroup to northern Siberia and that the expansion of the D2b branch occurred in Beringia ~7,000 years ago. In general, a detailed analysis of mtDNA gene pools of northern Asians provides the additional evidence to rule out the existence of a northern Asian route for the initial human colonization of Asia.}, }
@article {pmid17886373, year = {2007}, author = {Mats, AN and Znamenskaia, LF and Fattiakhetdinova, ZA and Iashin, MM and Denisova, EV and Trofimova, IB}, title = {[Affinoleukin in combined treatment of psoriasis].}, journal = {Zhurnal mikrobiologii, epidemiologii i immunobiologii}, volume = {}, number = {4}, pages = {32-38}, pmid = {17886373}, issn = {0372-9311}, mesh = {Adolescent ; Adult ; Aged ; Anti-Inflammatory Agents/therapeutic use ; Biological Products/administration &amp; dosage/chemistry/*therapeutic use ; Cell Count ; Drug Therapy, Combination ; Female ; Humans ; Immunosuppressive Agents/therapeutic use ; Injections, Subcutaneous ; Killer Cells, Natural/immunology ; Leukocytes/chemistry ; Male ; Membrane Proteins/administration &amp; dosage/chemistry/isolation &amp; purification/therapeutic use ; Middle Aged ; Molecular Weight ; Monocytes/cytology ; Psoriasis/blood/*drug therapy ; Receptors, Antigen, T-Cell, gamma-delta ; T-Lymphocyte Subsets/immunology ; Treatment Outcome ; }, abstract = {Open randomized controlled clinical trials in which 161 patients were included showed that Affinoleukin (commercial drug which contains low molecular weight proteins obtained from extract of human leukocytes membranes) was effective in treatment of psoriasis. Affinoleukin, when added to regular treatment, accelerated the establishment of remission and its duration by restoration of impaired regulatory and defensive functions of T-lymphocytes, particularly, gamma deltaT- and NKT-cells as well as monocytes and NK-cells. Regular treatment led to marked positive effect in 45% of patients with severe psoriasis and psoriasis of intermediate severity. Addition of Affinoleukin in combined treatment increased proportion of patients with marked positive effect to 72%. Magnitude of Affinoleukin effect was directly related with its dose and severity of psoriasis. Course of Affinoleukin injections (up to 30 units in adults) is worthwhile to use as immunobiological component of combined treatment of exacerbations of severe psoriasis and psoriasis of intermediate severity.}, }
@article {pmid17867504, year = {2007}, author = {Denisova, TV and Kazeev, KSh}, title = {[The influence of variable and constant magnetic fields on biota and biological activity of ordinary chernozem soils].}, journal = {Radiatsionnaia biologiia, radioecologiia}, volume = {47}, number = {3}, pages = {345-348}, pmid = {17867504}, issn = {0869-8031}, mesh = {Electromagnetic Fields/*adverse effects ; Radiation Monitoring/*methods ; Russia ; *Soil/analysis/standards ; *Soil Microbiology/standards ; }, abstract = {In model experiments on influence variable magnetic fields of industrial frequency (50 Hz) an induction of 1500 and of 6000 mkTl and the constant magnetic field an induction of 6000 mkTl and of 15000 mkTl during 5 days of exposure on biological properties of chernozem ordinary is shown, that the soil microflora is more sensitive to magnetic fields, than enzymes activity. Bacteria are more sensitive, than microscopic mushrooms. Dehydrogenase it is steady against influence of all variants. Constant magnetic field by the induction of 15000 mkTl rendered practically identical authentic overwhelming influence on catalase and saccharase activity - on 51 and 47% accordingly.}, }
@article {pmid17706584, year = {2007}, author = {Denisova, OV and Chernov, AV and Koledachkina, TY and Matvienko, NI}, title = {A tag-based approach for high-throughput analysis of CCWGG methylation.}, journal = {Analytical biochemistry}, volume = {369}, number = {2}, pages = {154-160}, doi = {10.1016/j.ab.2007.06.048}, pmid = {17706584}, issn = {0003-2697}, mesh = {Animals ; Base Sequence ; *DNA Methylation ; DNA Primers/*genetics ; DNA-Cytosine Methylases/*genetics/metabolism ; Deoxyribonucleases, Type II Site-Specific/*genetics/metabolism ; Electrophoresis, Polyacrylamide Gel ; Genomics/*methods ; Humans ; Reproducibility of Results ; }, abstract = {Non-CpG methylation occurring in the context of CNG sequences is found in plants at a large number of genomic loci. However, there is still little information available about non-CpG methylation in mammals. Efficient methods that would allow detection of scarcely localized methylated sites in small quantities of DNA are required to elucidate the biological role of non-CpG methylation in both plants and animals. In this study, we tested a new whole genome approach to identify sites of CCWGG methylation (W is A or T), a particular case of CNG methylation, in genomic DNA. This technique is based on digestion of DNAs with methylation-sensitive restriction endonucleases EcoRII-C and AjnI. Short DNAs flanking methylated CCWGG sites (tags) are selectively purified and assembled in tandem arrays of up to nine tags. This allows high-throughput sequencing of tags, identification of flanking regions, and their exact positions in the genome. In this study, we tested specificity and efficiency of the approach.}, }
@article {pmid17703276, year = {2007}, author = {Derenko, M and Malyarchuk, B and Denisova, G and Wozniak, M and Grzybowski, T and Dambueva, I and Zakharov, I}, title = {Y-chromosome haplogroup N dispersals from south Siberia to Europe.}, journal = {Journal of human genetics}, volume = {52}, number = {9}, pages = {763-770}, pmid = {17703276}, issn = {1434-5161}, mesh = {Chromosomes, Human, Y/*classification/*genetics ; Europe ; Genetic Variation ; *Genetics, Population ; *Haplotypes ; Humans ; Male ; Microsatellite Repeats ; Phylogeny ; Polymorphism, Single Nucleotide ; Siberia ; }, abstract = {In order to reconstruct the history of Y-chromosome haplogroup (hg) N dispersals in north Eurasia, we have analyzed the diversity of microsatellite (STR) loci within two major hg N clades, N2 and N3, in a total sample of 1,438 males from 17 ethnic groups, mainly of Siberian and Eastern European origin. Based on STR variance analysis we observed that hg N3a is more diverse in Eastern Europe than in south Siberia. However, analysis of median networks showed that there are two STR subclusters of hg N3a, N3a1 and N3a2, that are characterized by different genetic histories. Age calculation of STR variation within subcluster N3a1 indicated that its first expansion occurred in south Siberia [approximately 10,000 years (ky)] and then this subcluster spread into Eastern Europe where its age is around 8 ky ago. Meanwhile, younger subcluster N3a2 originated in south Siberia (probably in the Baikal region) approximately 4 ky ago. Median network and variance analyses of STR haplotypes suggest that south Siberian N3a2 haplotypes spread further into Volga-Ural region undergoing serial bottlenecks. In addition, median network analysis of STR data demonstrates that haplogroup N2-A is represented by two subclusters, showing recent expansion times. The data obtained allow us to suggest Siberian origin of haplogroups N3 and N2 that are currently widespread in some populations of Eastern Europe.}, }
@article {pmid17694818, year = {2006}, author = {Vasil'ev, AIu and Tsyganova, LA and Ol'khova, EB and Vykliuk, MV and Serova, NS and Denisova, RB}, title = {[Radiation diagnosis for tuberculous gonitis in children].}, journal = {Vestnik rentgenologii i radiologii}, volume = {}, number = {5}, pages = {37-40}, pmid = {17694818}, issn = {0042-4676}, mesh = {Adolescent ; Arthrography/*methods ; Child ; Child, Preschool ; Diagnosis, Differential ; Humans ; Infant ; *Knee Joint ; Male ; Reproducibility of Results ; Severity of Illness Index ; Tuberculosis, Osteoarticular/*diagnostic imaging ; Ultrasonography ; }, abstract = {Tuberculous knee joint lesion in children is the most common form of articular pathology. The high sociomedical significance of this disease in modern society is determined by not only its prevalence, but also economic losses caused by high disability rates in children. The purpose of the study was to determine the possibilities and place of a comprehensive radiation study in the diagnosis of tuberculous gonitis in children. Sixty patients aged 10 months to 18 years were examined. Clinical studies were comprehensively assessed, by taking into account the collected history data and orhthopedic and laboratory findings. Surgery was made in 26 children. All the patients underwent mutiprojection X-ray study, knee joint ultrasound study being made in 48 patients. Knee joint X-ray study was performed on Siregraph (Siemens) apparatuses. Real-time ultrasonography was conducted on Aloka SSD-1100 and Aloka--3500+ apparatuses, by employing a 7.5-MHz linear transducer. According to P.G. Kornev (1971), three phases were identified in the course of tuberculous gonitis. These included prearthritic (the development of primary ostitis); arthritic (articular cartilage destruction, followed by the development of secondary arthritis; and postarthritic (sequels of prior tuberculous osteomyelitis) phases. Analysis indicated that the use of the routine X-ray study in combination with ultrasound scanning in the diagnosis of tuberculous gonitis in children yielded more adequate information on tissue and knee joint structural changes and made it possible to define further policy treatment and to predict the outcomes of the disease.}, }
@article {pmid17677236, year = {2007}, author = {Denisov, SI and Kostur, M and Denisova, ES and Hänggi, P}, title = {Analytically solvable model of a driven system with quenched dichotomous disorder.}, journal = {Physical review. E, Statistical, nonlinear, and soft matter physics}, volume = {75}, number = {6 Pt 1}, pages = {061123}, doi = {10.1103/PhysRevE.75.061123}, pmid = {17677236}, issn = {1539-3755}, abstract = {We perform a time-dependent study of the driven dynamics of overdamped particles that are placed in a one-dimensional, piecewise linear random potential. This setup of spatially quenched disorder then exerts a dichotomous varying random force on the particles. We derive the path integral representation of the resulting probability density function for the position of the particles and transform this quantity of interest into the form of a Fourier integral. In doing so, the evolution of the probability density can be investigated analytically for finite times. It is demonstrated that the probability density contains both a delta -singular contribution and a regular part. While the former part plays a dominant role at short times, the latter rules the behavior at large evolution times. The slow approach of the probability density to a limiting Gaussian form as time tends to infinity is elucidated in detail.}, }
@article {pmid17603677, year = {2006}, author = {Cheknev, SB and Babaeva, EE and Denisova, EA and Vorob'eva, UA and Mongush, EM}, title = {Preparation of specimens of human serum gamma-globulin modified by copper and zinc and its immunochemical characteristics.}, journal = {Bulletin of experimental biology and medicine}, volume = {142}, number = {6}, pages = {710-713}, doi = {10.1007/s10517-006-0458-8}, pmid = {17603677}, issn = {0007-4888}, mesh = {Cations/chemistry ; Copper/*chemistry ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunohistochemistry ; Protein Binding ; Protein Conformation ; Proteins/chemistry ; Spectrophotometry, Ultraviolet ; Ultrafiltration ; Zinc/*chemistry ; gamma-Globulins/*chemistry/*immunology ; }, abstract = {Specimens of human serum gamma-globulin modified by molar excess of copper and zinc cations were obtained by molecular ultrafiltration. Conformation characteristics of the protein were determined by UV spectrophotometry. Immunochemical study included radial immunodiffusion test and direct and sandwich enzyme-linked immunosorbent assay. After binding of copper and zinc, the gamma-globulin molecule underwent conformation changes modifying presentation of antigenic determinants on the globule surface and their availability for recognition by specific antibodies. The effects of copper were much more pronounced than those of zinc cations.}, }
@article {pmid17486763, year = {2007}, author = {Derenko, MV and Maliarchuk, BA and Wozniak, M and Denisova, GA and Dambueva, IK and Dorzhu, CM and Grzybowski, T and Zakharov, IA}, title = {[Distribution of the male lineages of Genghis Khan's descendants in northern Eurasian populations].}, journal = {Genetika}, volume = {43}, number = {3}, pages = {422-426}, pmid = {17486763}, issn = {0016-6758}, mesh = {Asian Continental Ancestry Group/*genetics ; Chromosomes, Human, Y/*genetics ; Ethnic Groups/*genetics ; Humans ; Male ; Microsatellite Repeats ; Mongolia/ethnology ; Siberia/ethnology ; }, abstract = {Data on the variation of 12 microsatellite loci of Y-chromosome haplogroup C3 were used to screen lineages included in the cluster of Genghis Khan's descendants in 18 northern Eurasian populations (Altaian Kazakhs, Altaians-Kizhi, Teleuts, Khakassians, Shorians, Tyvans, Todjins, Tofalars, Sojots, Buryats, Khamnigans, Evenks, Mongols, Kalmyks, Tajiks, Kurds, Persians, and Russians; the total sample size was 1437 people). The highest frequency of haplotypes from the cluster of the Genghis Khan's descendants was found in Mongols (34.8%). In Russia, this cluster was found in Altaian Kazakhs (8.3%), Altaians (3.4%), Buryats (2.3%), Tyvans (1.9%), and Kalmyks (1.7%).}, }
@article {pmid17405285, year = {2006}, author = {Denisova, TV}, title = {[Egineering psychology and ergonomics in aviation].}, journal = {Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine}, volume = {40}, number = {6}, pages = {59-63}, pmid = {17405285}, issn = {0233-528X}, mesh = {Aerospace Medicine/*trends ; *Aviation ; Ergonomics/*methods ; Humans ; Russia ; }, }
@article {pmid17402591, year = {2007}, author = {Bugaeva, LI and Denisova, TD and Spasov, AA}, title = {[Transplacental effects of bemithyl].}, journal = {Eksperimental'naia i klinicheskaia farmakologiia}, volume = {70}, number = {1}, pages = {40-43}, pmid = {17402591}, issn = {0869-2092}, mesh = {Animals ; Antioxidants/metabolism/*pharmacology ; Behavior, Animal/drug effects ; Benzimidazoles/metabolism/*pharmacology ; Female ; Fetal Development/drug effects ; Fetus/*drug effects ; Placenta/*metabolism ; Pregnancy ; Rats ; Rats, Inbred Strains ; }, abstract = {The daily administration of bemithyl (20 mg/kg) from 6 th to 16 th day of pregnancy in female rats led to the decrease in fetal death after the implantation and increased fetal body weight. The treatment of pregnant rats also led to acceleration of the development of physical condition and sensomotor reflexes of progeny in the postnatal period.}, }
@article {pmid17195631, year = {2006}, author = {Denisova, LB and Vorontsova, SV and Iaurova, NV}, title = {[Potentialities of new radiation technologies (ultrasonography, computed tomography, magnetic resonance imaging) in the diagnosis of endocrine diseases].}, journal = {Vestnik rentgenologii i radiologii}, volume = {}, number = {1}, pages = {29-43}, pmid = {17195631}, issn = {0042-4676}, mesh = {Diagnosis, Differential ; *Endocrine System Diseases/diagnostic imaging/pathology ; Humans ; Magnetic Resonance Imaging/*methods ; Reproducibility of Results ; Tomography, X-Ray/*methods ; Ultrasonography ; }, abstract = {In the paper providing the data available in the literature and many years' experience gained by the Department of the Moscow Regional Clinical Research Institute, the authors describe the potentialities of the currently available radiation techniques in the diagnosis of surgical endocrine diseases. The incidence of various organ dysfunctions is presented. The authors also consider the introscopic symptoms of various endocrinopathies and outline the common specific features of the ultrasound, computed tomographic, and magnetic resonance imaging pattern of individual masses (including adipose tissue cysts and tumors). Emphasis is laid on the leading role of ultrasonography (USG) in the diagnosis of thyroid and parathyroid diseases and on the priorities of computed tomography (CT) and magnetic resonance imaging (MRI) in visualIzing the abnormal thymus, mediastinally ectopic glands, and pituitary tumors. The capacities of each technique in the diagnosis of endocrine space lesions of the pancreas and adrenals are defined. The increase in the frequency of endocrine abnormalities accidentally identified at USG, CT, and MRI, which is noted in the paper, fosters the development of new trends in endocrinology and points to the need for screening surveys (particularly patients who live in poor environmental areas).}, }
@article {pmid17195527, year = {2006}, author = {Kulagin, AD and Lisukov, IA and Kriuchkova, IV and Sizikova, SA and Gilevich, AV and Denisova, VV and Filimonov, PN and Meniaeva, EV and Pronkina, NV and Kozhevnikov, VS and Chernykh, ER and Kozlov, VA}, title = {[Diagnosis and treatment of aquired aplastic anemia].}, journal = {Terapevticheskii arkhiv}, volume = {78}, number = {11}, pages = {48-54}, pmid = {17195527}, issn = {0040-3660}, mesh = {Anemia, Aplastic/*diagnosis/drug therapy/*therapy ; Antilymphocyte Serum/therapeutic use ; *Bone Marrow Transplantation ; Combined Modality Therapy ; Cyclosporine/therapeutic use ; Female ; Humans ; *Immunosuppression ; Immunosuppressive Agents ; Male ; Treatment Outcome ; }, abstract = {AIM: To analyse the results of diagnosis and treatment of patients with aquired aplastic anemia (AA) in one center.<br><br>MATERIAL AND METHODS: All AA patients, diagnosed and treated in one clinic in 1998-2005, were included in the trial. In severe and very severe AA (SAA/VSAA) the patients (n = 19) received combined immunosuppressive therapy (IST) with antithymocytic globulin (ATG) and cyclosporin A (CsA), in non-severe AA (NSAA) the patients (n = 9) were given monotherapy with CsA. Allogenic transplantation of bone marrow (alloTBM) was made in 4 young patients with SAA/VSAA.<br><br>RESULTS: The diagnosis of AA was established in 33 patients (19 males and 14 females): NSAA in 9, SAA in 19, VSAA in 5, idiopathic--in 26, posthepatic--in 5, associated with pregnancy--in 2 patients. Age median was 20 years (13-53). The clone of paroxysmal nocturnal hemoglobinuria (PNH) was identified in 7 of 33 patients (21%), antigen HLA-DRB1 *15 in 6 of 11 patients (55%). In median of 26-month follow-up 31 patients (94%) were alive. In IST, complete or partial remissions were obtained in 88% patients. Median of the interval to achievement of transfusion independence made up 2.5 months. All the patients after alloTBM are in complete remission, chronic extensive transplant against host reaction was observed in one case.<br><br>CONCLUSION: Introduction of updated protocols provides long-term survival of more than 80% AA patients. To optimize treatment outcomes, it is necessary to include newly diagnosed AA patients into ongoing multicenter studies.}, }
@article {pmid17181064, year = {2006}, author = {Babaeva, EE and Vorobyova, UA and Denisova, EA and Medvedeva, DA and Cheknev, SB}, title = {Binding of zinc cations to human serum gamma-globulin.}, journal = {Bulletin of experimental biology and medicine}, volume = {141}, number = {5}, pages = {602-605}, doi = {10.1007/s10517-006-0232-y}, pmid = {17181064}, issn = {0007-4888}, mesh = {Cations/*metabolism ; Globins/chemistry/*metabolism ; Humans ; Phenanthrolines ; Protein Binding ; Protein Conformation ; Spectrophotometry, Ultraviolet ; Ultrafiltration ; Zinc/analysis/*metabolism ; }, abstract = {Binding of zinc cations to human serum gamma-globulin was studied by molecular ultrafiltration. The content of free metal in the filtrate was evaluated by reaction with o-phenanthroline. Conformation characteristics of the protein were determined by UV spectrophotometry. Our findings suggest that gamma-globulin molecule contains several zinc binding sites differing by corresponding constants and successively occupied with increase in the content of bound metal. The parameters of zinc binding correspond to those obtained in experiments with copper. Conformation status of protein with bound zinc differs significantly from that of protein with bound copper cations.}, }
@article {pmid17114825, year = {2007}, author = {Paley, EL and Denisova, G and Sokolova, O and Posternak, N and Wang, X and Brownell, AL}, title = {Tryptamine induces tryptophanyl-tRNA synthetase-mediated neurodegeneration with neurofibrillary tangles in human cell and mouse models.}, journal = {Neuromolecular medicine}, volume = {9}, number = {1}, pages = {55-82}, pmid = {17114825}, issn = {1559-1174}, support = {EB01850/EB/NIBIB NIH HHS/United States ; }, mesh = {Animals ; Behavior, Animal/drug effects ; Brain/drug effects/metabolism/*pathology ; Cell Count ; Cell Line, Tumor ; Glucose/metabolism ; Hippocampus/metabolism/pathology ; Humans ; Male ; Mice ; Mice, Inbred BALB C ; Motor Activity/drug effects ; Neurodegenerative Diseases/metabolism/*pathology/psychology ; Neurofibrillary Tangles/*pathology ; Neuroglia/pathology ; Neurons/pathology ; Tryptamines/pharmacology/*physiology ; Tryptophan-tRNA Ligase/antagonists &amp; inhibitors/*physiology ; }, abstract = {The neuropathological hallmarks of Alzheimer's disease (AD) and other taupathies include neurofibrillary tangles and plaques. Despite the fact that only 2-10% of AD cases are associated with genetic mutations, no nontransgenic or metabolic models have been generated to date. The findings of tryptophanyl-tRNA synthetase (TrpRS) in plaques of the AD brain were reported recently by the authors. Here it is shown that expression of cytoplasmic-TrpRS is inversely correlated with neurofibrillary degeneration, whereas a nonionic detergent-insoluble presumably aggregated TrpRS is simultaneously accumulated in human cells treated by tryptamine, a metabolic tryptophan analog that acts as a competitive inhibitor of TrpRS. TrpRSN- terminal peptide self-assembles in double-helical fibrils in vitro. Herein, tryptamine causes neuropathy characterized by motor and behavioral deficits, hippocampal neuronal loss, neurofibrillary tangles, amyloidosis, and glucose decrease in mice. Tryptamine induced the formation of helical fibrillary tangles in both hippocampal neurons and glia. Taken together with the authors' previous findings of tryptamine-induced nephrotoxicity and filamentous tangle formation in kidney cells, the authors' data indicates a general role of tryptamine in cell degeneration and loss. It is concluded that tryptamine as a component of a normal diet can induce neurodegeneration at the concentrations, which might be consumed along with food. Tryptophan-dependent tRNAtrp aminoacylation catalyzed by TrpRS can be inhibited by its substrate tryptophan at physiological concentrations was demonstrated. These findings indicate that the dietary supplementation with tryptophan as a tryptamine competitor may not counteract the deleterious influence of tryptamine. The pivotal role of TrpRS in protecting against neurodegeneration is suggested, providing an insight into the pathogenesis and a possible treatment of neurodegenerative diseases.}, }
@article {pmid17076067, year = {2006}, author = {Denisova, K and Singh, M and Kowler, E}, title = {The role of part structure in the perceptual localization of a shape.}, journal = {Perception}, volume = {35}, number = {8}, pages = {1073-1087}, doi = {10.1068/p5518}, pmid = {17076067}, issn = {0301-0066}, mesh = {Distance Perception/physiology ; Form Perception/*physiology ; Humans ; *Optical Illusions ; Psychometrics ; Psychophysics ; }, abstract = {The process of object localization may be accomplished with respect to a particular reference location, such as the center of gravity, COG (eg Vishwanath and Kowler, 2003 Vision Research 43 1637-1653). Here, we investigated how part structure affects an object's reference location. The reference location was evaluated with a measure of the illusory displacement of an internal target element embedded within a larger object (Morgan et al, 1990 Vision Research 30 1793-1810). To examine whether the reference location is different for shapes with part structure, two shapes were tested: circle (small and large; no part structure) and bell (shape with two parts, one larger than the other). Results were examined with respect to two predictions: either the location of an object is based on its shape as a whole, disregarding part structure (ie a single, overall COG), or the parts are processed separately (different COGs). With the circles, the results showed a systematic illusory displacement of the internal target toward the COG. With the bell, the illusion was significantly weaker than with both circles--even though the main part of the bell had the same size as the small circle, and its horizontal axis had the same extent as the large circle. Moreover, the distance judgments for the bell were consistent with a (weaker) reference point being located at the COG of the larger part, rather than at the COG of the entire bell. These results show that the part structure of a shape plays a role in the representation of its location, and that for complex shapes the perceived location of an embedded element depends more on the parts within which it is embedded, rather than on the whole shape.}, }
@article {pmid16881369, year = {2006}, author = {Denisova, LN}, title = {[Diet therapy of rheumatoid arthritis].}, journal = {Terapevticheskii arkhiv}, volume = {78}, number = {6}, pages = {84-88}, pmid = {16881369}, issn = {0040-3660}, mesh = {Arthritis, Rheumatoid/*diet therapy ; Humans ; }, }
@article {pmid16850925, year = {2006}, author = {Karal'nik, BV and Denisova, TG and Plazun, AA and Duĭsenova, RB}, title = {[Antigen-binding lymphocytes of tuberculin specificity in M. bovis-infected rabbits in the dynamics of treatment for tuberculosis].}, journal = {Problemy tuberkuleza i boleznei legkikh}, volume = {}, number = {5}, pages = {48-53}, pmid = {16850925}, issn = {1728-2993}, mesh = {Animals ; Antigen Presentation ; Mycobacterium bovis/*immunology ; Rabbits ; T-Lymphocyte Subsets/immunology ; T-Lymphocytes/*immunology ; Tuberculin/*immunology ; Tuberculosis/*immunology/*therapy ; }, abstract = {In M. bovis-infected rabbits, antigen-binding lymphocytes (ABL) of tuberculin specificity were detected within 7-105 days after inoculation. Due to etiotropic chemotherapy, ABL disappeared 31-45 days following inoculation. At the trial's end, the seeding rate and morphological changes in the organs of M. bovis-infected correlated with the content of ABL 105 days after inoculation. The early disappearance of ABL in the rats is regarded as an index of the efficiency of treatment for tuberculosis.}, }
@article {pmid16637277, year = {2006}, author = {Rechkina, EA and Denisova, GF and Masalova, OV and Lideman, LF and Denisov, DA and Lesnova, EI and Ataullakhanov, RI and Gur'ianova, SV and Kushch, AA}, title = {[Epitope mapping of antigenic determinants of hepatitis C virus proteins by phage display].}, journal = {Molekuliarnaia biologiia}, volume = {40}, number = {2}, pages = {357-368}, pmid = {16637277}, issn = {0026-8984}, mesh = {Amino Acid Sequence ; Animals ; Antibodies, Viral/*analysis/immunology ; *Epitope Mapping/methods ; Epitopes/*chemistry/genetics/immunology ; Female ; Hepacivirus/*chemistry/genetics/immunology ; Humans ; Mice ; Molecular Sequence Data ; *Peptide Library ; Protein Structure, Secondary/genetics ; Protein Structure, Tertiary/genetics ; RNA Helicases/chemistry/genetics/immunology ; Serine Endopeptidases/chemistry/genetics/immunology ; Viral Nonstructural Proteins/*chemistry/genetics/immunology ; }, abstract = {Study of individual hepatitis C (HCV) proteins could help to find a molecular structure and conformation, localization of antigenic and immunogenic determinants, to reveal of protective epitopes. It is necessary for practical medicine - development of diagnostic test-systems, vaccines and therapeutics. Linear and conformation dependent epitopes of HCV proteins was localized in this work and immunogenic properties of phage displayed peptides screened on monoclonal antibodies to HCV proteins have been investigated. Eleven epitopes of four HCV proteins have been studied. Three epitopes was found as linear, two epitopes were dependent on secondary structure of proteins and one epitope was dependent on tertiary structure of NS3 protein. Aminoacid sequences of other determinants have been determined and the distinct localization of these determinants will be continued after discovering of tertiary structure of HCV proteins. It was shown, that phage mimotope 3f4 is immunogenic and could induce specific hu- moral immune response to NS5A HCV protein. The data obtained could be useful for improving of HCV diagnostic test-systems, studying of amino acid substitutions and its influence on antigenic properties of the HCV proteins. The results could help to study an immune response in patients infected with different genotypes of HCV. Phage displayed peptides mimicking the antigenic epitopes of HCV proteins could be applied to development of HCV vaccine.}, }
@article {pmid16616781, year = {2007}, author = {Paley, EL and Smelyanski, L and Malinovskii, V and Subbarayan, PR and Berdichevsky, Y and Posternak, N and Gershoni, JM and Sokolova, O and Denisova, G}, title = {Mapping and molecular characterization of novel monoclonal antibodies to conformational epitopes on NH2 and COOH termini of mammalian tryptophanyl-tRNA synthetase reveal link of the epitopes to aggregation and Alzheimer's disease.}, journal = {Molecular immunology}, volume = {44}, number = {4}, pages = {541-557}, doi = {10.1016/j.molimm.2006.02.006}, pmid = {16616781}, issn = {0161-5890}, mesh = {Alzheimer Disease/immunology ; Amino Acid Sequence ; Animals ; Antibodies, Monoclonal/chemistry/*immunology ; Antibody Specificity ; Cattle ; Cell Line, Tumor ; Cross Reactions ; Epitope Mapping ; *Epitopes/chemistry/genetics/immunology ; Humans ; Mice ; Mice, Inbred BALB C ; Models, Molecular ; Molecular Sequence Data ; Organ Specificity ; Protein Conformation ; Rabbits ; Species Specificity ; Tryptophan-tRNA Ligase/chemistry/*immunology ; }, abstract = {Tryptophanyl-tRNA synthetase (TrpRS) is an interferon-induced phosphoprotein with autoantigenic and cytokine activities detected in addition to its canonical function in tRNA aminoacylation. The availability of monoclonal antibodies (mAbs) specific for TrpRS is important for development of tools for TrpRS monitoring. A molecular characterization of two mAbs raised in mice, using purified, enzymatically active bovine TrpRS as the inoculating antigen, is presented in this report. These IgG1 antibodies are specific for bovine, human and rabbit but not E. coli TrpRS. Immunoreactivity and specificity of mAbs were verified with purified recombinant hTrpRS expressed in E. coli and TrpRS-derived synthetic peptides. One of the mAbs, 9D7 is able to disaggregate fibrils formed by Ser32-Tyr50 TrpRS-peptide. Epitope mapping revealed that disaggregation ability correlates with binding of 9D7 to this peptide in ELISA and immunocytochemistry. This epitope covers a significant part of N-terminal extension that suggested to be proteolytically deleted in vivo from the full-length TrpRS whereas remaining COOH-fragment possesses a cytokine activity. For epitope mapping of mAb 6C10, the affinity selected phage-displayed peptides were used as a database for prediction of conformational discontinuous epitopes within hTrpRS crystal structure. Using computer algorithm, this epitope is attributed to COOH-terminal residues Asp409-Met425. In immunoblotting, the 6C10 mAb reacts preferably with (i) oligomer than monomer, and (ii) bound than free TrpRS forms. The hTrpRS expression was shown to correlate with growth rates of neuroblastoma and pancreatic cancer cells. Immunohistochemically both mAbs revealed extracellular plaque-like aggregates in hippocampus of Alzheimer's disease brain.}, }
@article {pmid16579550, year = {2006}, author = {Denisova, TV and Kazeev, KSh}, title = {[The restoration of the enzyme activity of chernozem soil after gamma-irradiation].}, journal = {Radiatsionnaia biologiia, radioecologiia}, volume = {46}, number = {1}, pages = {89-93}, pmid = {16579550}, issn = {0869-8031}, mesh = {*Catalase/analysis/radiation effects ; *Gamma Rays ; Kinetics ; *Oxidoreductases/analysis/radiation effects ; Russia ; Soil/*analysis ; *beta-Fructofuranosidase/analysis/radiation effects ; }, abstract = {The Influence of gamma-radiation by dozes 1, 5, 10 and 20 kGy on enzyme activity of ordinary chemozem were studied. Dynamics of the restoration of the enzyme activity after the influence of gamma-radiation in model experiments in 3, 30, 90 and 180 days was investigated. The doze 1 kGy did no statistically significant influence on the investigated enzymes. Dehydrogenase is more radiosensitive enzyme than catalase. Values of the saccharase activity differed a significant variation so in most cases it has not been registered statistically significant changes. In 90-180 days of the incubation enzymes activity was restored up to control values. Dehydrogenase activity in 180 days in variants with dozes 10 and 20 kGy was restored up to a level of the control, over variants with dozes 1 and 5 kGy--is higher than the control over 78% and 23% accordingly. Saccharase activity in 180 days after the influence of gamma-radiation with a doze 20 kGy was on 61% lower than the control.}, }
@article {pmid16566435, year = {2006}, author = {Lebron, JA and Troilol, PJ and Pacchione, S and Griffiths, TG and Harper, LB and Mixson, LA and Jackson, BE and Michna, L and Barnum, AB and Denisova, L and Johnson, CN and Maurer, KL and Morgan-Hoffman, S and Niu, Z and Roden, DF and Wang, Z and Wolf, JJ and Hamilton, TR and Laux, KM and Soper, KA and Ledwith, BJ}, title = {Adaptation of the WHO guideline for residual DNA in parenteral vaccines produced on continuous cell lines to a limit for oral vaccines.}, journal = {Developments in biologicals}, volume = {123}, number = {}, pages = {35-44; discussion 55-73}, pmid = {16566435}, issn = {1424-6074}, mesh = {Administration, Oral ; Animals ; Cell Line ; Chlorocebus aethiops ; DNA/*administration &amp; dosage/*adverse effects/pharmacokinetics ; Deoxyribonucleases ; Endocytosis ; Endosomes/physiology ; Female ; Humans ; Male ; Practice Guidelines as Topic ; Vaccines/administration &amp; dosage/*standards ; Vero Cells ; World Health Organization ; }, abstract = {Although there is a WHO guidance for a limit on residual DNA for parenterally administered vaccines produced on continuous cell lines, there is no corresponding guidance for oral vaccines. To help determine an oral limit, we performed a study of Vero cell DNA uptake in rats, in which the relative uptake and persistence of Vero cell DNA administered orally was compared to its uptake when delivered intramuscularly (IM). The results of this study allowed the generation of an empirically derived IM versus oral factor (10(6)) representing the relative inefficiency of DNA uptake by oral administration. This factor was then applied to the WHO recommended parenteral limit of 10 ng/dose to determine a corresponding upper limit on the level of residual Vero cell DNA for an oral vaccine of 10 mg. As a conservative approach, this empirically determined limit was reduced 100-fold to 100 microg. Thus, the results of this animal study, together with additional evidence in the literature, support a residual DNA safety limit of 100 microg per dose for an oral vaccine produced on a continuous cell line.}, }
@article {pmid16297666, year = {2006}, author = {Grinshtein, N and Yang, TC and Parsons, R and Millar, J and Denisova, G and Dissanayake, D and Leitch, J and Wan, Y and Bramson, J}, title = {Recombinant adenovirus vaccines can successfully elicit CD8+ T cell immunity under conditions of extreme leukopenia.}, journal = {Molecular therapy : the journal of the American Society of Gene Therapy}, volume = {13}, number = {2}, pages = {270-279}, doi = {10.1016/j.ymthe.2005.09.018}, pmid = {16297666}, issn = {1525-0016}, mesh = {Adenoviridae/*genetics/*immunology ; Animals ; B-Lymphocytes/immunology ; CD4-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/*immunology/*pathology/virology ; Dendritic Cells/immunology ; Female ; Leukopenia/*immunology/pathology/*therapy ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Radiation Chimera ; Vaccines, Synthetic/administration &amp; dosage/immunology ; Viral Vaccines/*administration &amp; dosage/*immunology ; Whole-Body Irradiation ; }, abstract = {We have examined the efficacy of vaccination with recombinant adenovirus under conditions of extreme leukopenia in lethally irradiated mice reconstituted with autologous bone marrow. The expansion of antigen-specific CD8(+) T cells following immunization of lethally irradiated hosts paralleled the recovery of total CD8(+) T cells. Surprisingly, the numbers of antigen-specific CD8(+) T cells in lethally irradiated mice beyond 6 weeks postimmunization were comparable to the numbers found in nonirradiated controls. CD8(+) T cells elicited in the lethally irradiated hosts were functionally indistinguishable from those elicited in normal hosts. Antigen expression and presentation persisted for a longer period of time in the draining lymph nodes of irradiated mice compared to those of nonirradiated animals, suggesting that antigen presentation mechanisms were intact during the reconstitution period. Experiments employing allogeneic bone marrow demonstrated that radioresistant host antigen-presenting cells were responsible for antigen presentation during the process of immune reconstitution. These results demonstrate clear compatibility of adenovirus vaccines and cytotoxic therapy. Furthermore, these observations provide novel insights into the mechanisms of CD8(+) T cell activation following adenovirus immunization.}, }
@article {pmid16283003, year = {2005}, author = {Cheknev, SB and Babaeva, EE and Vorobeva, UA and Denisova, EA}, title = {Reactions between human serum gamma-globulin and zinc cations.}, journal = {Bulletin of experimental biology and medicine}, volume = {140}, number = {2}, pages = {209-212}, doi = {10.1007/s10517-005-0447-3}, pmid = {16283003}, issn = {0007-4888}, mesh = {Cations ; Dose-Response Relationship, Drug ; Humans ; Hydrogen-Ion Concentration ; Protein Binding ; Protein Conformation ; Proteins/chemistry ; Spectrophotometry ; Time Factors ; Ultraviolet Rays ; Zinc/*chemistry ; gamma-Globulins/*chemistry ; }, abstract = {Interactions of human serum gamma-globulin with zinc cations in solution were studied by differential spectrophotometry in UV light. Supraphysiological concentrations of zinc caused an increment in optical density of protein solution reflecting the effect of gamma-globulin saturation with the metal. Zinc concentrations below physiological led to hypochromism in the protein absorption spectrum. Conformation changes in gamma-globulin during interactions with zinc are analyzed for the surface and intramolecular binding sites and are compared with the effects of copper cations.}, }
@article {pmid16273932, year = {2005}, author = {Denisova, OI and Klimenko, GIa}, title = {[Logbook system application in regional multi-field hospital].}, journal = {Problemy sotsial'noi gigieny, zdravookhraneniia i istorii meditsiny}, volume = {}, number = {4}, pages = {38-41}, pmid = {16273932}, issn = {0869-866X}, mesh = {Hospital Information Systems ; Hospitals, General/*organization &amp; administration ; Humans ; *Medical Records Systems, Computerized ; Organizational Innovation ; Russia ; }, }
@article {pmid16261343, year = {2006}, author = {Derenko, M and Malyarchuk, B and Denisova, GA and Wozniak, M and Dambueva, I and Dorzhu, C and Luzina, F and Miścicka-Sliwka, D and Zakharov, I}, title = {Contrasting patterns of Y-chromosome variation in South Siberian populations from Baikal and Altai-Sayan regions.}, journal = {Human genetics}, volume = {118}, number = {5}, pages = {591-604}, pmid = {16261343}, issn = {0340-6717}, mesh = {*Chromosomes, Human, Y ; Ethnic Groups/*genetics ; *Genetics, Population ; Haplotypes ; Humans ; Male ; Siberia ; }, abstract = {In order to investigate the genetic history of autochthonous South Siberian populations and to estimate the contribution of distinct patrilineages to their gene pools, we have analyzed 17 Y-chromosomal binary markers (YAP, RPS4Y(711), SRY-8299, M89, M201, M52, M170, 12f2, M9, M20, 92R7, SRY-1532, DYS199, M173, M17, Tat, and LLY22 g) in a total sample of 1,358 males from 14 ethnic groups of Siberia (Altaians-Kizhi, Teleuts, Shors, Tuvinians, Todjins, Tofalars, Sojots, Khakassians, Buryats, Evenks), Central/Eastern Asia (Mongolians and Koreans) and Eastern Europe (Kalmyks and Russians). Based on both, the distribution pattern of Y-chromosomal haplogroups and results on AMOVA analysis we observed the statistically significant genetic differentiation between the populations of Baikal and Altai-Sayan regions. We suggest that these regional differences can be best explained by different contribution of Central/Eastern Asian and Eastern European paternal lineages into gene pools of modern South Siberians. The population of the Baikal region demonstrates the prevalence of Central/Eastern Asian lineages, whereas in the populations of Altai and Sayan regions the highest paternal contribution resulted from Eastern European descent is revealed. Yet, our data on Y-chromosome STRs variation demonstrate the clear differences between the South Siberian and Eastern European R1a1-lineages with the evolutionary ages compatible with divergence time between these two regional groups.}, }
@article {pmid16250592, year = {2005}, author = {Lideman, LF and Kasennova, EV and Denisova, GF and Stakhanova, VM and Zverev, SIa and Gibadulin, RA and Bobkov, AF}, title = {[Anti-CD4 autoimmunity in HIV-infected persons in Russia].}, journal = {Voprosy virusologii}, volume = {50}, number = {5}, pages = {15-19}, pmid = {16250592}, issn = {0507-4088}, mesh = {Amino Acid Sequence ; Autoantibodies/*blood ; CD4 Antigens/*immunology ; Consensus Sequence ; Epitopes, T-Lymphocyte ; Female ; Gene Products, nef/genetics ; HIV Infections/*blood/*virology ; HIV-1/*genetics ; Human Immunodeficiency Virus Proteins ; Humans ; Male ; Molecular Sequence Data ; Species Specificity ; Substance Abuse, Intravenous/blood ; Viral Regulatory and Accessory Proteins/genetics ; nef Gene Products, Human Immunodeficiency Virus ; }, abstract = {The frequency of anti-CD4 antibodies was determined in the sera or plasma derived from the patients infected with HIV-1 belonging to different genetic subgroups. The anti-CD4-antibodies in a dilution of > or = 1:1000 were found in 14% of the patients infected with the gagA/envA virus characteristic for injectable drug users in East Europe. The frequency of autoimmune antibodies among the HIV-infected patients with envB virus was substantially less (4.4%). Competitive ELISA using monoclonal antibodies to different CD4 domains demonstrated that irrespective of the viral genotype, the autoimmune epitope is located within the D4 or D3/D4 domains of CD4 receptor.}, }
@article {pmid16201340, year = {2005}, author = {Snetkova, EV and Levin, VM and Petroniuk, IuS and Denisov, AF and Bogachenkov, AN and Denisova, LA and Khramtsova, EA}, title = {[Application of the method of acoustic microscopy for the study of eye tissues].}, journal = {Morfologiia (Saint Petersburg, Russia)}, volume = {127}, number = {2}, pages = {72-75}, pmid = {16201340}, issn = {1026-3543}, mesh = {Animals ; Coturnix/anatomy &amp; histology/embryology ; Embryo, Nonmammalian/anatomy &amp; histology ; Eye/*anatomy &amp; histology ; Goats/anatomy &amp; histology ; Larva/anatomy &amp; histology ; Microscopy, Acoustic/*methods ; Ranidae/anatomy &amp; histology ; }, abstract = {The paper describes the results of evaluation of potentialities of the application of scanning acoustic microscopy, which is still relatively new and as yet uncommon method of biological research, for the supravital study of animal eye structure in species with different eyeball size. Using the eyes of a goat, quail embryo (in situ) and frog tadpole (in vivo) as the examples, it was shown that non-destructive nature of the method used allows for the study of peculiarities of major morphologic structures in the state that is close to the natural one. Some acoustic images are presented showing eye structural elements along with the results of quantitative characteristic of the cornea, sclera and iris.}, }
@article {pmid16078530, year = {2005}, author = {Igolkina, SN and Puzyrev, VF and Zinina, LG and Denisova, NM and Burkov, AN and Obriadina, AP and Ulanova, TI}, title = {[The enzyme immunological test systems "DS-EIA-HBeAg" and "DS-EIA-anti-HBe" for the specific diagnosis and prediction of the outcomes of acute and chronic hepatitis B].}, journal = {Klinicheskaia laboratornaia diagnostika}, volume = {}, number = {6}, pages = {34-37}, pmid = {16078530}, issn = {0869-2084}, mesh = {Acute Disease ; Clinical Laboratory Techniques ; Enzyme-Linked Immunosorbent Assay/methods ; Hepatitis B/*diagnosis/physiopathology ; Hepatitis B e Antigens/*blood ; Hepatitis B, Chronic/*diagnosis/physiopathology ; Humans ; Predictive Value of Tests ; Prognosis ; }, }
@article {pmid16078433, year = {2005}, author = {Lashkevich, VA and Koroleva, GA and Lukashev, AN and Denisova, EV and Katargina, LA and Khoroshilova-Maslova, IP}, title = {[Acute enterovirus uveitis in infants].}, journal = {Voprosy virusologii}, volume = {50}, number = {3}, pages = {36-45}, pmid = {16078433}, issn = {0507-4088}, mesh = {Animals ; Antibodies, Viral/blood ; Antigens, Viral/immunology ; Cataract/etiology ; Cross Reactions ; Disease Models, Animal ; *Disease Outbreaks ; *Echovirus Infections/blood/complications/diagnosis/epidemiology ; Enterovirus B, Human/genetics/immunology ; Eye/virology ; Glaucoma/etiology ; Humans ; Infant ; Iris/pathology ; Molecular Epidemiology ; Neutralization Tests ; Phylogeny ; Primates ; Pupil Disorders ; RNA, Viral/genetics ; Russia/epidemiology ; *Uveitis/blood/complications/diagnosis/epidemiology ; Vision Disorders/etiology ; }, abstract = {Enterovirus uveitis (EU) is a new infant eye disease that was first detected and identified in Russia in 1980-1981. Three subtypes of human echoviruses (EV19K, EV11A, and EV11/B) caused 5 nosocomial outbreaks of EU in different Siberian cities and towns in 1980-1989, by affecting more than 750 children mainly below one year of age. Sporadic and focal EU cases (more than 200) were also retrospectively diagnosed in other regions of Russia and in different countries of the former Soviet Union. There were following clinical manifestations: common symptoms of the infection; acute uveitis (rapid focal iridic destruction, pupillary deformities, formation of membranes in the anterior chamber of the eye); and in 15-30% of cases severe complications, cataract, glaucoma, vision impairments. Uveitis strains EV19 and EV11 caused significant uveitis in primates after inoculation into the anterior chamber of the eye, as well as sepsis-like fatal disease with liver necrosis after venous infection. The uveitis strains are phylogenetically and pathogenetically close for primates to strains EV19 and EV11 isolated from young children with sepsis-like disease. The contents of this review have been published in the Reviews in Medical Virology, 2004, vol. 14, p. 241-254.}, }
@article {pmid16044839, year = {2005}, author = {Pogozheva, AV and Denisova, NN and Derbeneva, SA and Anykina, NV and Kondakova, NM}, title = {[Application of nutritional formulare in the dietotherapy in patients with cardiovascular diseases].}, journal = {Voprosy pitaniia}, volume = {74}, number = {3}, pages = {32-38}, pmid = {16044839}, issn = {0042-8833}, mesh = {Adult ; Aged ; Cardiovascular Diseases/*diet therapy ; *Enteral Nutrition ; Female ; *Food, Formulated ; Humans ; Lipid Metabolism ; Male ; Middle Aged ; Obesity/*diet therapy ; }, abstract = {It was found that the including of nutritional formulae in the dietary therapy for patients with cardiovascular diseases and obesity has been evaluated, had a positive effect on the clinical presentation of disease, lipid metabolism and the dynamic of body composition of patients.}, }
@article {pmid16034353, year = {2005}, author = {Garashchenko, TI and Denisova, OA and Kotov, RV}, title = {[Initial antibiotic therapy in acute otitis media and acute sinusitis in children].}, journal = {Vestnik otorinolaringologii}, volume = {}, number = {3}, pages = {62-63}, pmid = {16034353}, issn = {0042-4668}, mesh = {Acute Disease ; Administration, Oral ; Amoxicillin/administration &amp; dosage/*therapeutic use ; Anti-Bacterial Agents/administration &amp; dosage/*therapeutic use ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Otitis Media, Suppurative/*drug therapy/*epidemiology/microbiology ; Sinusitis/*drug therapy/*epidemiology/microbiology ; Suspensions ; Treatment Outcome ; }, }
@article {pmid15974299, year = {2004}, author = {Malyarchuk, B and Derenko, M and Grzybowski, T and Lunkina, A and Czarny, J and Rychkov, S and Morozova, I and Denisova, G and Miścicka-Sliwka, D}, title = {Differentiation of mitochondrial DNA and Y chromosomes in Russian populations.}, journal = {Human biology}, volume = {76}, number = {6}, pages = {877-900}, doi = {10.1353/hub.2005.0021}, pmid = {15974299}, issn = {0018-7143}, mesh = {Chromosomes, Human, Y/*genetics ; DNA, Mitochondrial/*genetics ; European Continental Ancestry Group/genetics ; Female ; Genetic Heterogeneity ; Genetic Variation ; *Genetics, Population ; Haplotypes ; Humans ; Male ; *Polymorphism, Genetic ; Polymorphism, Restriction Fragme